RLG00000006537

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013999
Physical Location & Seq
Forward (+)
8226934 .. 8227728
795 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006537

Sequence Viewer

Length: 795 bp
ATGGCAGCCAAGACCCCTCATGACTCGTCCTTCTCATTTTCCAGAAGGTACTTCTGGAAAAAGAAGGTCGACGATGATGAAGACGACGACCACGAAGCCCTCAACTTCAGTTCCTACTCACATTTCTCCGAGCAAGAGAAAGAAGAGGAGCTAGTCCGAAACATACATATGTCAGCAACAACCGATCAACAAATTGCATCAGTCCTGCCTGAAGTGCCACGGAAGAAACACTCACTGGTCTCAGTCTCTAAGCTCCGGTCAGCTCTTACGCTATTCGGCAAGAGCCGTTCTGGCTCTCGCTCCGGTTTAATGGGTACTCTTTTCGGGTATCGCCGCGGGCATGTTCACTTGGCATTTCAGGAGGATCCCAAGTTGAACCCTACGTTTCTCATCGAGCTGGCTACGCCAACTAGTGTTCTGGTTCGAGAAATGGCTTCGGGGTTGGTCAGGATTGCGTTGGAGTGTGAGAAGAAGACTGATCATAAGAAGAAGGGGTTGAAGTTGCTGGAGGAGCCGCTGTGGAGGACTTACTGCAATGGCAAGAAGTGCGGTTTCGCTATGAGGCGAGATTGTGGTCCAGAGGAGTGGAAGGTGCTGAAAGCTCTGGAGCCCGTTTCAATGGGGGCTGGGGTTTTACCTGGGAATGAAGATGGAGCTGGATCTGAAGGTGAGCTCATGTATATGAGAGCGAAGTTTGAGAGAGTTGTGGGGTCCAAGGACTCAGAGGCCTTTTACATGATGAACCCTGATGGTTCTGGTGGTCCTGAACTCAGTGTTTACTTGCTTAGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

265

Amino Acids

29.66

Weight (kDa)

7.7

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 104 - 263 2e-73 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25640 AT3G25640
fragaria_vesca FvH4_4g28270
malus_domestica MD13G1048700.v1.1 MD16G1050000.v1.1
prunus_persica Prupe.1G303400_v2.0.a1
pyrus_communis pycom16g04380
rosa_chinensis RchiOBHm_Chr4g0436141
rosa_laevigata RLG00000006537
rosa_multiflora Rmu_sc0022632.1_g000001
rosa_roxburghii Rroxscaffold_5G00377240
rosa_rugosa Rorug04G0292200
rosa_samantha Rh4AG346200 Rh4BG355300 Rh4CG369900 Rh4DG348900
rosa_wichuraiana Rw4G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 69
AccII CGCG 1 cut(s) 336
AciI CCGC 4 cut(s) 334, 336, 515, 549
AclWI GGATC 3 cut(s) 359, 372, 667
AcuI CTGAAG 3 cut(s) 91, 231, 684
AfaI GTAC 2 cut(s) 50, 316
AgsI TTSAA 3 cut(s) 376, 499, 618
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 1 cut(s) 637
AluBI AGCT 7 cut(s) 151, 253, 263, 397, 602, 656, 673
AluI AGCT 7 cut(s) 151, 253, 263, 397, 602, 656, 673
Alw21I GWGCWC 1 cut(s) 675
Alw26I GTCTC 2 cut(s) 244, 250
AlwI GGATC 3 cut(s) 359, 372, 667
AoxI GGCC 1 cut(s) 726
ApeKI GCWGC 1 cut(s) 5
AspS9I GGNCC 3 cut(s) 575, 711, 761
AsuHPI GGTGA 1 cut(s) 680
AvaII GGWCC 3 cut(s) 575, 711, 761
BamHI GGATCC 1 cut(s) 364
BanII GRGCYC 2 cut(s) 612, 675
BbsI GAAGAC 2 cut(s) 87, 479
Bbv12I GWGCWC 1 cut(s) 675
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 644, 743
BceAI ACGGC 1 cut(s) 270
BciT130I CCWGG 1 cut(s) 639
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 244, 250
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 3 cut(s) 152, 411, 793
BglI GCCNNNNNGGC 1 cut(s) 291
BisI GCNGC 3 cut(s) 6, 334, 515
BlsI GCNGC 3 cut(s) 7, 335, 516
Bme1390I CCNGG 1 cut(s) 639
Bme18I GGWCC 3 cut(s) 575, 711, 761
BmgT120I GGNCC 3 cut(s) 575, 711, 761
BmiI GGNNCC 4 cut(s) 366, 513, 609, 712
BmrFI CCNGG 1 cut(s) 639
BmsI GCATC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 87, 479
BpmI CTGGAG 2 cut(s) 527, 626
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 4 cut(s) 218, 334, 638, 714
BsaWI WCCGGW 2 cut(s) 255, 302
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
Bse1I ACTGG 1 cut(s) 240
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 1 cut(s) 639
BseDI CCNNGG 4 cut(s) 218, 334, 638, 714
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 3 cut(s) 255, 735, 784
BseNI ACTGG 1 cut(s) 240
BseRI GAGGAG 3 cut(s) 161, 524, 596
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 626
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 675
BsiSI CCGG 2 cut(s) 256, 303
BsmAI GTCTC 2 cut(s) 244, 250
BsnI GGCC 1 cut(s) 728
Bso31I GGTCTC 1 cut(s) 244
Bsp1286I GDGCHC 2 cut(s) 612, 675
Bsp143I GATC 4 cut(s) 184, 364, 478, 659
BspACI CCGC 4 cut(s) 334, 336, 515, 549
BspANI GGCC 1 cut(s) 728
BspCNI CTCAG 3 cut(s) 254, 734, 783
BspFNI CGCG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 4 cut(s) 366, 513, 609, 712
BspPI GGATC 3 cut(s) 359, 372, 667
BspTNI GGTCTC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 541
BsrI ACTGG 1 cut(s) 240
BssECI CCNNGG 4 cut(s) 218, 334, 638, 714
BssMI GATC 4 cut(s) 184, 364, 478, 659
BssT1I CCWWGG 1 cut(s) 714
Bst2UI CCWGG 1 cut(s) 639
Bst6I CTCTTC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 546
BstC8I GCNNGC 2 cut(s) 338, 399
BstDEI CTNAG 5 cut(s) 241, 249, 721, 770, 785
BstDSI CCRYGG 2 cut(s) 218, 334
BstFNI CGCG 1 cut(s) 336
BstKTI GATC 4 cut(s) 187, 367, 481, 662
BstMAI GTCTC 2 cut(s) 244, 250
BstMBI GATC 4 cut(s) 184, 364, 478, 659
BstMWI GCNNNNNNNGC 5 cut(s) 214, 291, 403, 511, 546
BstNI CCWGG 1 cut(s) 639
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 637
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 2 cut(s) 87, 479
BstX2I RGATCY 2 cut(s) 364, 659
BstXI CCANNNNNNTGG 1 cut(s) 585
BstYI RGATCY 2 cut(s) 364, 659
BsuRI GGCC 1 cut(s) 728
BtgI CCRYGG 2 cut(s) 218, 334
BtsIMutI CAGTG 2 cut(s) 233, 778
Cac8I GCNNGC 2 cut(s) 338, 399
CciI TCATGA 1 cut(s) 19
Cfr13I GGNCC 3 cut(s) 575, 711, 761
Cfr42I CCGCGG 1 cut(s) 337
Csp6I GTAC 2 cut(s) 49, 315
CviAII CATG 4 cut(s) 20, 341, 676, 736
CviQI GTAC 2 cut(s) 49, 315
DdeI CTNAG 5 cut(s) 241, 249, 721, 770, 785
DpnI GATC 4 cut(s) 186, 366, 480, 661
DpnII GATC 4 cut(s) 184, 364, 478, 659
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Ecl136II GAGCTC 1 cut(s) 673
Eco130I CCWWGG 1 cut(s) 714
Eco147I AGGCCT 1 cut(s) 728
Eco24I GRGCYC 2 cut(s) 612, 675
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 3 cut(s) 575, 711, 761
Eco53kI GAGCTC 1 cut(s) 673
Eco57I CTGAAG 3 cut(s) 91, 231, 684
EcoICRI GAGCTC 1 cut(s) 673
EcoRII CCWGG 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 714
EcoT38I GRGCYC 2 cut(s) 612, 675
ErhI CCWWGG 1 cut(s) 714
FaeI CATG 4 cut(s) 23, 344, 679, 739
FatI CATG 4 cut(s) 19, 340, 675, 735
FauI CCCGC 1 cut(s) 329
FauNDI CATATG 1 cut(s) 168
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 6, 334, 515
FriOI GRGCYC 2 cut(s) 612, 675
Fsp4HI GCNGC 3 cut(s) 6, 334, 515
FspBI CTAG 3 cut(s) 152, 411, 793
GluI GCNGC 3 cut(s) 6, 334, 515
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 2 cut(s) 527, 626
HaeIII GGCC 1 cut(s) 728
HapII CCGG 2 cut(s) 256, 303
Hin1II CATG 4 cut(s) 23, 344, 679, 739
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 3 cut(s) 23, 719, 789
HpaII CCGG 2 cut(s) 256, 303
HphI GGTGA 1 cut(s) 680
Hpy166II GTNNAC 3 cut(s) 70, 346, 778
Hpy188I TCNGA 4 cut(s) 130, 158, 664, 724
Hpy188III TCNNGA 9 cut(s) 20, 42, 55, 359, 425, 448, 578, 605, 764
Hpy8I GTNNAC 3 cut(s) 70, 346, 778
Hpy99I CGWCG 2 cut(s) 74, 89
HpyAV CCTTC 6 cut(s) 39, 40, 58, 484, 583, 659
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 2 cut(s) 197, 534
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 291, 403, 511, 546
HpyF3I CTNAG 5 cut(s) 241, 249, 721, 770, 785
HpySE526I ACGT 1 cut(s) 383
Hsp92II CATG 4 cut(s) 23, 344, 679, 739
Ksp22I TGATCA 1 cut(s) 478
KspI CCGCGG 1 cut(s) 337
Kzo9I GATC 4 cut(s) 184, 364, 478, 659
LmnI GCTCC 6 cut(s) 148, 258, 305, 511, 607, 653
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 206
MaeI CTAG 3 cut(s) 152, 411, 793
MaeII ACGT 1 cut(s) 383
MalI GATC 4 cut(s) 186, 366, 480, 661
MboI GATC 4 cut(s) 184, 364, 478, 659
MboII GAAGA 7 cut(s) 92, 155, 235, 481, 484, 499, 659
MflI RGATCY 2 cut(s) 364, 659
MhlI GDGCHC 2 cut(s) 612, 675
MluCI AATT 1 cut(s) 192
MlyI GAGTC 2 cut(s) 17, 713
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 9 cut(s) 27, 110, 139, 355, 502, 516, 555, 574, 718
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 2 cut(s) 167, 680
MspA1I CMGCKG 2 cut(s) 336, 517
MspI CCGG 2 cut(s) 256, 303
MspR9I CCNGG 1 cut(s) 639
MvaI CCWGG 1 cut(s) 639
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 5 cut(s) 214, 291, 403, 511, 546
NdeI CATATG 1 cut(s) 168
NdeII GATC 4 cut(s) 184, 364, 478, 659
NlaIII CATG 4 cut(s) 23, 344, 679, 739
NlaIV GGNNCC 4 cut(s) 366, 513, 609, 712
NspI RCATGY 1 cut(s) 344
PagI TCATGA 1 cut(s) 19
PceI AGGCCT 1 cut(s) 728
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PkrI GCNGC 3 cut(s) 7, 335, 516
PleI GAGTC 2 cut(s) 17, 713
PpsI GAGTC 2 cut(s) 17, 713
Psp124BI GAGCTC 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 637
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 1 cut(s) 637
PspN4I GGNNCC 4 cut(s) 366, 513, 609, 712
PspPI GGNCC 3 cut(s) 575, 711, 761
PsuI RGATCY 2 cut(s) 364, 659
RsaI GTAC 2 cut(s) 50, 316
RsaNI GTAC 2 cut(s) 49, 315
RseI CAYNNNNRTG 2 cut(s) 167, 680
SacI GAGCTC 1 cut(s) 675
SacII CCGCGG 1 cut(s) 337
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 3 cut(s) 6, 334, 515
Sau3AI GATC 4 cut(s) 184, 364, 478, 659
Sau96I GGNCC 3 cut(s) 575, 711, 761
SchI GAGTC 2 cut(s) 17, 713
ScrFI CCNGG 1 cut(s) 639
SduI GDGCHC 2 cut(s) 612, 675
SfaNI GCATC 1 cut(s) 206
Sfr303I CCGCGG 1 cut(s) 337
SgrBI CCGCGG 1 cut(s) 337
SinI GGWCC 3 cut(s) 575, 711, 761
SmiMI CAYNNNNRTG 2 cut(s) 167, 680
SpeI ACTAGT 1 cut(s) 410
Sse9I AATT 1 cut(s) 192
SseBI AGGCCT 1 cut(s) 728
SsiI CCGC 4 cut(s) 334, 336, 515, 549
SspMI CTAG 3 cut(s) 152, 411, 793
SstI GAGCTC 1 cut(s) 675
StuI AGGCCT 1 cut(s) 728
StyD4I CCNGG 1 cut(s) 637
StyI CCWWGG 1 cut(s) 714
TaiI ACGT 1 cut(s) 386
TaqI TCGA 3 cut(s) 69, 393, 424
TasI AATT 1 cut(s) 192
TauI GCSGC 2 cut(s) 336, 517
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 2 cut(s) 240, 778
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 3 cut(s) 93, 660, 755
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 2 cut(s) 240, 778
VpaK11BI GGWCC 3 cut(s) 575, 711, 761
XceI RCATGY 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 3 cut(s) 152, 411, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.