AT3G26750

HECT-like Ubiquitin-conjugating enzyme (E2)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015450
Physical Location & Seq
Reverse (-)
9839925 .. 9842079
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G26750.1

Sequence Viewer

Length: 1581 bp
ATGAAAAATCGAAGCAGAGCAATTTCTAATTCCATGGAGCAAAACTCTAAAACCCAGAGGACATGGAGATACACATGGGAAGCTCAATCTCATTCTCCAAACCTTCGTCTCTTTCTTTTCGATTCCAAAACTAACCCTAAGATCCACTGCAAAAGCCTCAATGTATCAACCATCGTCGGAAAATCTCAACTTCTCGTCACATGGATCAACGAAGAAGATGAAGAAGCTGCAAGCAAAGAAGAGATTGTTTCTCTTCTAGTTCCTATCCCTAGGGTCTTACTAGATACTGAATCTCCTGTAAATTTCAAAGCTTTAGACGATCACATCGAGGTTAGACTTGTTCTGTTACTCCCTGTTGATCATCCTTTAGTCTCAGACTTCAATTTGGTGACTGATTCTAGAGAAAAGTCGGCGCCTTTAGTGATGGGTTATGATCTAAAGACTCTATCTTTAATGGGAGGAGTTCATTTTTACTGTAGAAGTTGTTCAAATAGGTTAACAAAGAAGGAACTTTTAGATTTCTCAGAAATGCCGTCTATTAATTGGAGAGAATCAGCTGATAATTGGTTTGGTACTTGTTGTTGTTCATTTGGTGGAATTAGTGAGAAGATGGTTGTTAAGTACACGAATTCGTATACTTGCTCTAGTGGGTTGTGTTTACTTAGTGCTACTACTGTGTTGCTTAGCAAAGATGATCTTGTAGAGTGTATCTTGTCTGAGAAAGGTGGAACTGAAGTCGAATTTGAGTCGAGTTTAGCTTTGAGTTGTGATGTTGGTGTAGTTGAACCGGGTTCTAGAATTAGTGAAGGAAATGCTGAGAGTCATGAGAGTGGAGGTGAGAATGTTTGTGGTCAAGTGGATGAATCGAAGACGCGTTGTATAGATAAGGCATCACTACCTGGATGTTGTGTACATGACTCGCCTGACTCTAATGAGAGTGTTCAGCTGGAGGAGAAGAAACTTACACTTGATAAGAAGTTTCTTCTTGATGGGTTTCTTGAGGATGTTTTTATGGCGAAAGCGTCAAATGTGTCAAAGAATGTCGAGTGGATTGAATTTGCTTGCCCTGAGTGTTCCTCTCCTCTTGGAGCCTATCCTTCTGGTGTTGGGAGCAACGGTAAACCCATTGATGGTGGAGTTAGACTCTTCAAATGCTATATTTCCACATCATCGACGACGGGTGAATCCTCTGACGTTTTCAGGAAATACACATTGGAAAGAATGTTTACGAATCAGCTAGTGGAATGCTCCAAAGAGGAGTTATCATTTCACGTGTTGGTCAAAGATTTGACAACTAAATCACCTCTGTTCAACATCGTTATTTTGAATCCAAACACATTTTCCTCGACTGGTCTTTGCTCGAGCCAAGATGAACCAGGTTCCGCATTAGAGTTAAGCGCTATTGTGAAGGTTCTGTTTTCTGATTGCAACAGCTCGGTAGTGAAGAAGATTGATGAAGAAGTATACATTTTGAAGGGACAAGGAGAGGAGTTGATAGAGTTAATAACGAATGCAAGCAAATTTCTTCCATCTTCGTGTTCATATCTTCAAGGCGCACTTGTCTCATCGATGCATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT_2

Protein Analysis

526

Amino Acids

57.94

Weight (kDa)

4.99

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT_2 PF09814 144 - 517 3.5e-20 HECT-like Ubiquitin-conjugating enzyme (E2)-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26750
fragaria_vesca FvH4_1g12740
malus_domestica MD15G1254500.v1.1
prunus_persica Prupe.7G159100_v2.0.a1 Prupe.7G159100_v2.0.a1
pyrus_communis pycom15g22360
rosa_chinensis RchiOBHm_Chr2g0100811
rosa_laevigata RLG00000017013
rosa_multiflora Rmu_sc0001659.1_g000020
rosa_roxburghii Rroxscaffold_2G00141840
rosa_rugosa Rorug02G0093000
rosa_samantha Rh2AG140600 Rh2BG145600 Rh2CG146600 Rh2DG146000
rosa_wichuraiana Rw2G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 412
AccI GTMKAC 2 cut(s) 635, 1464
AccII CGCG 1 cut(s) 874
AciI CCGC 1 cut(s) 1383
AclWI GGATC 2 cut(s) 136, 212
AcsI RAATTY 5 cut(s) 301, 628, 740, 1055, 1520
AcuI CTGAAG 1 cut(s) 753
AcvI CACGTG 1 cut(s) 1273
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 3 cut(s) 574, 623, 912
AfeI AGCGCT 1 cut(s) 1399
AfiI CCNNNNNNNGG 1 cut(s) 1130
AflIII ACRYGT 2 cut(s) 872, 1272
AjnI CCWGG 2 cut(s) 898, 1375
AjuI GAANNNNNNNTTGG 4 cut(s) 1196, 1228, 1324, 1356
AluBI AGCT 8 cut(s) 83, 227, 311, 557, 758, 946, 1237, 1434
AluI AGCT 8 cut(s) 83, 227, 311, 557, 758, 946, 1237, 1434
Alw26I GTCTC 3 cut(s) 113, 376, 1567
AlwI GGATC 2 cut(s) 136, 212
Ama87I CYCGRG 1 cut(s) 1360
Aor51HI AGCGCT 1 cut(s) 1399
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 5 cut(s) 301, 628, 740, 1055, 1520
AseI ATTAAT 1 cut(s) 540
Asp700I GAANNNNTTC 1 cut(s) 484
AspA2I CCTAGG 1 cut(s) 269
AspLEI GCGC 3 cut(s) 415, 1400, 1556
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 4 cut(s) 400, 848, 1193, 1293
AvaI CYCGRG 1 cut(s) 1360
AvrII CCTAGG 1 cut(s) 269
BanI GGYRCC 1 cut(s) 412
BbrPI CACGTG 1 cut(s) 1273
BbsI GAAGAC 1 cut(s) 875
BbvI GCAGC 1 cut(s) 214
BccI CCATC 6 cut(s) 179, 418, 604, 983, 1124, 1537
BceAI ACGGC 1 cut(s) 517
BciT130I CCWGG 2 cut(s) 900, 1377
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 3 cut(s) 113, 376, 1567
BfaI CTAG 7 cut(s) 257, 270, 281, 399, 645, 795, 1238
BfmI CTRYAG 1 cut(s) 475
BfoI RGCGCY 2 cut(s) 416, 1401
BisI GCNGC 1 cut(s) 228
BlnI CCTAGG 1 cut(s) 269
BlpI GCTNAGC 1 cut(s) 683
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 3 cut(s) 789, 900, 1377
BmeT110I CYCGRG 1 cut(s) 1360
BmiI GGNNCC 3 cut(s) 414, 1090, 1381
BmrFI CCNGG 3 cut(s) 789, 900, 1377
BmsI GCATC 2 cut(s) 899, 1560
BpiI GAAGAC 1 cut(s) 875
BplI GAGNNNNNCTC 6 cut(s) 29, 61, 1061, 1093, 1128, 1160
BpmI CTGGAG 1 cut(s) 968
Bpu1102I GCTNAGC 1 cut(s) 683
BpuEI CTTGAG 1 cut(s) 1019
BpuMI CCSGG 1 cut(s) 789
Bsa29I ATCGAT 1 cut(s) 1568
BsaAI YACGTR 1 cut(s) 1273
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 33, 269
BsaXI ACNNNNNCTCC 4 cut(s) 277, 307, 1102, 1132
Bsc4I CCNNNNNNNGG 1 cut(s) 1130
Bse1I ACTGG 1 cut(s) 1354
BseBI CCWGG 2 cut(s) 900, 1377
BseCI ATCGAT 1 cut(s) 1568
BseDI CCNNGG 2 cut(s) 33, 269
BseGI GGATG 4 cut(s) 361, 865, 908, 1009
BseLI CCNNNNNNNGG 1 cut(s) 1130
BseMII CTCAG 5 cut(s) 387, 537, 708, 807, 1059
BseNI ACTGG 1 cut(s) 1354
BseRI GAGGAG 5 cut(s) 474, 965, 1071, 1271, 1502
BseXI GCAGC 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 874
BshNI GGYRCC 1 cut(s) 412
BshVI ATCGAT 1 cut(s) 1568
BsiHKCI CYCGRG 1 cut(s) 1360
BsiSI CCGG 1 cut(s) 788
BslFI GGGAC 1 cut(s) 1491
BslI CCNNNNNNNGG 1 cut(s) 1130
BsmAI GTCTC 3 cut(s) 113, 376, 1567
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 1491
BsmI GAATGC 2 cut(s) 1250, 1516
BsoBI CYCGRG 1 cut(s) 1360
Bsp1407I TGTACA 1 cut(s) 910
Bsp143I GATC 6 cut(s) 141, 204, 319, 358, 433, 694
Bsp1720I GCTNAGC 1 cut(s) 683
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 1 cut(s) 1383
BspCNI CTCAG 5 cut(s) 386, 536, 709, 808, 1060
BspDI ATCGAT 1 cut(s) 1568
BspFNI CGCG 1 cut(s) 874
BspHI TCATGA 1 cut(s) 823
BspLI GGNNCC 3 cut(s) 414, 1090, 1381
BspPI GGATC 2 cut(s) 136, 212
BspT107I GGYRCC 1 cut(s) 412
BsrGI TGTACA 1 cut(s) 910
BsrI ACTGG 1 cut(s) 1354
BssECI CCNNGG 2 cut(s) 33, 269
BssMI GATC 6 cut(s) 141, 204, 319, 358, 433, 694
BssNAI GTATAC 2 cut(s) 636, 1465
BssNI GRCGYC 1 cut(s) 413
BssT1I CCWWGG 2 cut(s) 33, 269
Bst1107I GTATAC 2 cut(s) 636, 1465
Bst2UI CCWGG 2 cut(s) 900, 1377
Bst4CI ACNGT 3 cut(s) 476, 676, 1118
Bst6I CTCTTC 3 cut(s) 234, 258, 1151
BstACI GRCGYC 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 910
BstBAI YACGTR 1 cut(s) 1273
BstC8I GCNNGC 3 cut(s) 232, 1063, 1516
BstDEI CTNAG 8 cut(s) 138, 373, 523, 662, 683, 717, 816, 1068
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 4 cut(s) 361, 865, 908, 1009
BstFNI CGCG 1 cut(s) 874
BstH2I RGCGCY 2 cut(s) 416, 1401
BstHHI GCGC 3 cut(s) 415, 1400, 1556
BstKTI GATC 6 cut(s) 144, 207, 322, 361, 436, 697
BstMAI GTCTC 3 cut(s) 113, 376, 1567
BstMBI GATC 6 cut(s) 141, 204, 319, 358, 433, 694
BstNI CCWGG 2 cut(s) 900, 1377
BstSCI CCNGG 3 cut(s) 787, 898, 1375
BstSFI CTRYAG 1 cut(s) 475
BstUI CGCG 1 cut(s) 874
BstV1I GCAGC 1 cut(s) 214
BstV2I GAAGAC 1 cut(s) 875
BstX2I RGATCY 1 cut(s) 141
BstYI RGATCY 1 cut(s) 141
BstZ17I GTATAC 2 cut(s) 636, 1465
Bsu15I ATCGAT 1 cut(s) 1568
BsuTUI ATCGAT 1 cut(s) 1568
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 4 cut(s) 361, 865, 908, 1009
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 3 cut(s) 232, 1063, 1516
CciI TCATGA 1 cut(s) 823
CfoI GCGC 3 cut(s) 415, 1400, 1556
ClaI ATCGAT 1 cut(s) 1568
CseI GACGC 2 cut(s) 880, 1011
CsiI ACCWGGT 1 cut(s) 1375
Csp6I GTAC 3 cut(s) 573, 622, 911
CviAII CATG 6 cut(s) 34, 63, 75, 201, 824, 914
CviQI GTAC 3 cut(s) 573, 622, 911
DdeI CTNAG 8 cut(s) 138, 373, 523, 662, 683, 717, 816, 1068
DinI GGCGCC 1 cut(s) 414
DpnI GATC 6 cut(s) 143, 206, 321, 360, 435, 696
DpnII GATC 6 cut(s) 141, 204, 319, 358, 433, 694
Eam1104I CTCTTC 3 cut(s) 234, 258, 1151
EarI CTCTTC 3 cut(s) 234, 258, 1151
Eco130I CCWWGG 2 cut(s) 33, 269
Eco47III AGCGCT 1 cut(s) 1399
Eco57I CTGAAG 1 cut(s) 753
Eco72I CACGTG 1 cut(s) 1273
Eco88I CYCGRG 1 cut(s) 1360
EcoRI GAATTC 1 cut(s) 628
EcoRII CCWGG 2 cut(s) 898, 1375
EcoT14I CCWWGG 2 cut(s) 33, 269
EcoT22I ATGCAT 1 cut(s) 1575
EgeI GGCGCC 1 cut(s) 414
EheI GGCGCC 1 cut(s) 414
ErhI CCWWGG 2 cut(s) 33, 269
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 6 cut(s) 37, 66, 78, 204, 827, 917
FalI AAGNNNNNCTT 4 cut(s) 681, 713, 1542, 1574
FaqI GGGAC 1 cut(s) 1491
FatI CATG 6 cut(s) 33, 62, 74, 200, 823, 913
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 2 cut(s) 635, 1464
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 4 cut(s) 348, 872, 915, 1016
Fsp4HI GCNGC 1 cut(s) 228
FspBI CTAG 7 cut(s) 257, 270, 281, 399, 645, 795, 1238
GlaI GCGC 3 cut(s) 414, 1399, 1555
GluI GCNGC 1 cut(s) 228
GsuI CTGGAG 1 cut(s) 968
HaeII RGCGCY 2 cut(s) 416, 1401
HapII CCGG 1 cut(s) 788
HgaI GACGC 2 cut(s) 880, 1011
HhaI GCGC 3 cut(s) 415, 1400, 1556
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 6 cut(s) 37, 66, 78, 204, 827, 917
Hin6I GCGC 3 cut(s) 413, 1398, 1554
HinP1I GCGC 3 cut(s) 413, 1398, 1554
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HindIII AAGCTT 1 cut(s) 309
HpaI GTTAAC 1 cut(s) 498
HpaII CCGG 1 cut(s) 788
HphI GGTGA 4 cut(s) 400, 848, 1193, 1293
Hpy166II GTNNAC 8 cut(s) 498, 624, 636, 659, 911, 1121, 1227, 1465
Hpy188I TCNGA 6 cut(s) 179, 376, 526, 718, 1192, 1423
Hpy188III TCNNGA 6 cut(s) 399, 795, 824, 986, 998, 1201
Hpy8I GTNNAC 8 cut(s) 498, 624, 636, 659, 911, 1121, 1227, 1465
Hpy99I CGWCG 3 cut(s) 179, 1177, 1180
HpyAV CCTTC 6 cut(s) 113, 499, 800, 1107, 1402, 1468
HpyCH4III ACNGT 3 cut(s) 476, 676, 1118
HpyCH4IV ACGT 2 cut(s) 1194, 1272
HpyCH4V TGCA 5 cut(s) 150, 230, 1428, 1514, 1573
HpyF3I CTNAG 8 cut(s) 138, 373, 523, 662, 683, 717, 816, 1068
HpySE526I ACGT 2 cut(s) 1194, 1272
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 6 cut(s) 37, 66, 78, 204, 827, 917
HspAI GCGC 3 cut(s) 413, 1398, 1554
KasI GGCGCC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 358
KspAI GTTAAC 1 cut(s) 498
Kzo9I GATC 6 cut(s) 141, 204, 319, 358, 433, 694
LmnI GCTCC 4 cut(s) 37, 1088, 1110, 1253
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 2 cut(s) 899, 1560
MabI ACCWGGT 1 cut(s) 1375
MaeI CTAG 7 cut(s) 257, 270, 281, 399, 645, 795, 1238
MaeII ACGT 2 cut(s) 1194, 1272
MaeIII GTNAC 3 cut(s) 196, 345, 388
MalI GATC 6 cut(s) 143, 206, 321, 360, 435, 696
MboI GATC 6 cut(s) 141, 204, 319, 358, 433, 694
MflI RGATCY 1 cut(s) 141
MluI ACGCGT 1 cut(s) 872
Mly113I GGCGCC 1 cut(s) 413
MlyI GAGTC 6 cut(s) 436, 755, 829, 911, 920, 1137
MmeI TCCRAC 1 cut(s) 157
Mph1103I ATGCAT 1 cut(s) 1575
MroXI GAANNNNTTC 1 cut(s) 484
MseI TTAA 6 cut(s) 452, 497, 540, 618, 1394, 1502
MslI CAYNNNNRTG 2 cut(s) 828, 1534
MspA1I CMGCKG 2 cut(s) 557, 946
MspI CCGG 1 cut(s) 788
MspR9I CCNGG 3 cut(s) 789, 900, 1377
Mva1269I GAATGC 2 cut(s) 1250, 1516
MvaI CCWGG 2 cut(s) 900, 1377
MvnI CGCG 1 cut(s) 874
NarI GGCGCC 1 cut(s) 413
NciI CCSGG 1 cut(s) 789
NcoI CCATGG 1 cut(s) 33
NdeII GATC 6 cut(s) 141, 204, 319, 358, 433, 694
NlaIII CATG 6 cut(s) 37, 66, 78, 204, 827, 917
NlaIV GGNNCC 3 cut(s) 414, 1090, 1381
NmuCI GTSAC 2 cut(s) 196, 388
NsiI ATGCAT 1 cut(s) 1575
PaeR7I CTCGAG 1 cut(s) 1360
PagI TCATGA 1 cut(s) 823
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 2 cut(s) 1250, 1516
PdmI GAANNNNTTC 1 cut(s) 484
PfeI GAWTC 8 cut(s) 122, 290, 395, 551, 863, 1184, 1231, 1327
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 6 cut(s) 436, 754, 828, 911, 920, 1137
PluTI GGCGCC 1 cut(s) 416
PmaCI CACGTG 1 cut(s) 1273
PmlI CACGTG 1 cut(s) 1273
PpsI GAGTC 6 cut(s) 436, 754, 828, 911, 920, 1137
Ppu21I YACGTR 1 cut(s) 1273
PshBI ATTAAT 1 cut(s) 540
Psp6I CCWGG 2 cut(s) 898, 1375
PspCI CACGTG 1 cut(s) 1273
PspGI CCWGG 2 cut(s) 898, 1375
PspN4I GGNNCC 3 cut(s) 414, 1090, 1381
PspXI VCTCGAGB 1 cut(s) 1360
PsrI GAACNNNNNNTAC 2 cut(s) 469, 501
PsuI RGATCY 1 cut(s) 141
PvuII CAGCTG 2 cut(s) 557, 946
RsaI GTAC 3 cut(s) 574, 623, 912
RsaNI GTAC 3 cut(s) 573, 622, 911
RseI CAYNNNNRTG 2 cut(s) 828, 1534
SaqAI TTAA 6 cut(s) 452, 497, 540, 618, 1394, 1502
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 6 cut(s) 141, 204, 319, 358, 433, 694
SchI GAGTC 6 cut(s) 436, 755, 829, 911, 920, 1137
ScrFI CCNGG 3 cut(s) 789, 900, 1377
SexAI ACCWGGT 1 cut(s) 1375
SfaNI GCATC 2 cut(s) 899, 1560
SfcI CTRYAG 1 cut(s) 475
SfoI GGCGCC 1 cut(s) 414
Sfr274I CTCGAG 1 cut(s) 1360
SlaI CTCGAG 1 cut(s) 1360
SmiMI CAYNNNNRTG 2 cut(s) 828, 1534
SmlI CTYRAG 2 cut(s) 998, 1360
SmoI CTYRAG 2 cut(s) 998, 1360
SsiI CCGC 1 cut(s) 1383
SspDI GGCGCC 1 cut(s) 412
SspMI CTAG 7 cut(s) 257, 270, 281, 399, 645, 795, 1238
StyD4I CCNGG 3 cut(s) 787, 898, 1375
StyI CCWWGG 2 cut(s) 33, 269
TaaI ACNGT 3 cut(s) 476, 676, 1118
TaiI ACGT 2 cut(s) 1197, 1275
TatI WGTACW 2 cut(s) 621, 910
TfiI GAWTC 8 cut(s) 122, 290, 395, 551, 863, 1184, 1231, 1327
Tru1I TTAA 6 cut(s) 452, 497, 540, 618, 1394, 1502
Tru9I TTAA 6 cut(s) 452, 497, 540, 618, 1394, 1502
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 2 cut(s) 196, 388
TseI GCWGC 1 cut(s) 227
Tsp45I GTSAC 2 cut(s) 196, 388
TspDTI ATGAA 8 cut(s) 17, 234, 455, 576, 876, 1386, 1470, 1530
TspRI CASTG 1 cut(s) 152
VspI ATTAAT 1 cut(s) 540
XapI RAATTY 5 cut(s) 301, 628, 740, 1055, 1520
XbaI TCTAGA 2 cut(s) 398, 794
XhoI CTCGAG 1 cut(s) 1360
XmaJI CCTAGG 1 cut(s) 269
XmiI GTMKAC 2 cut(s) 635, 1464
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 7 cut(s) 257, 270, 281, 399, 645, 795, 1238
Zsp2I ATGCAT 1 cut(s) 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.