Rorug02G0093000

HECT-like Ubiquitin-conjugating enzyme (E2)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015450
Physical Location & Seq
Reverse (-)
7642758 .. 7645669
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0093000.1

Sequence Viewer

Length: 630 bp
ATGGCAGCTCAGTCTGGTTGTTCTGCTCCGGTTGTGCCTTCTCTGCCGCCGCCAACTCCAAAGTCGCCGCCCCAGTACCCGGATTTGTATGGCAAGCGCAGAGAAACCGCCAGGGTTCAGATGCTTGAGAGGGAAATTGGGTTTCTTGAGGAGGAACTAAAATCTGTTGAACGCCTTCAACCTGCTTCCCGATGCTGCAAAGAGATTGCTGATTTTGTGACTGCGAATCCAGATCCGTTAATACCTACAAATCGAAAGAAACGACGATCCTGTCGCTTTTGGAAATGGCTCTGTCTTGGGTCATTTGTTAAGGAAAAATGCCACGCTGCTCCTGCTGCGGTTCATGCAGCGGTAAATGCTGTGGTTCATGCAGCGGTAAATGCTGTGGTTCATGCAGCGGTAAATGCGGTAAATGCTGTGGTTCATGCAGCGGTTCATGCTGTGGCTCATGCAGCGGTTCATGCTGTGGTTCATGTAACTGTAATTCGTGTTTCAGTTGCCCTTCACTACCAAAGTGGCGATGTTGCTGCTCATGTCCTCGATCCCGTTGCTGCAAAACTAGTTCATGCAGCAGAAATTGCTGCATTTTATCATCGTGTTCGTGTTCATGTCCCGATTGCTCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT_2

Protein Analysis

209

Amino Acids

22.52

Weight (kDa)

9.38

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-gamma PF00631 37 - 102 1.2e-11 GGL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26750
fragaria_vesca FvH4_1g12740
malus_domestica MD15G1254500.v1.1
prunus_persica Prupe.7G159100_v2.0.a1 Prupe.7G159100_v2.0.a1
pyrus_communis pycom15g22360
rosa_chinensis RchiOBHm_Chr2g0100811
rosa_laevigata RLG00000017013
rosa_multiflora Rmu_sc0001659.1_g000020
rosa_roxburghii Rroxscaffold_2G00141840
rosa_rugosa Rorug02G0093000
rosa_samantha Rh2AG140600 Rh2BG145600 Rh2CG146600 Rh2DG146000
rosa_wichuraiana Rw2G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 270
Acc36I ACCTGC 1 cut(s) 190
AclWI GGATC 3 cut(s) 227, 261, 536
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 1 cut(s) 79
AgsI TTSAA 2 cut(s) 170, 179
AhlI ACTAGT 1 cut(s) 559
AjnI CCWGG 1 cut(s) 110
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
AlwI GGATC 3 cut(s) 227, 261, 536
Asp700I GAANNNNTTC 1 cut(s) 174
AspLEI GCGC 1 cut(s) 99
AsuC2I CCSGG 1 cut(s) 80
BcgI CGANNNNNNTGC 4 cut(s) 509, 530, 543, 564
BciT130I CCWGG 1 cut(s) 112
BcnI CCSGG 1 cut(s) 80
BcuI ACTAGT 1 cut(s) 559
BfaI CTAG 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 80, 112
BmrFI CCNGG 2 cut(s) 80, 112
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 2 cut(s) 111, 182
BmuI ACTGGG 1 cut(s) 67
BpuEI CTTGAG 2 cut(s) 146, 167
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 111
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 73
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 164
BsiSI CCGG 2 cut(s) 29, 80
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 596
Bsp143I GATC 3 cut(s) 232, 266, 541
BspCNI CTCAG 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 3 cut(s) 227, 261, 536
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 3 cut(s) 232, 266, 541
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 481
BstAPI GCANNNNNTGC 1 cut(s) 578
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 9
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 3 cut(s) 235, 269, 544
BstMBI GATC 3 cut(s) 232, 266, 541
BstNI CCWGG 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 78, 110
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BtgZI GCGATG 1 cut(s) 534
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 1 cut(s) 95
CfoI GCGC 1 cut(s) 99
Csp6I GTAC 1 cut(s) 76
CviJI RGCY 3 cut(s) 8, 289, 446
CviKI_1 RGCY 3 cut(s) 8, 289, 446
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 1 cut(s) 9
DpnI GATC 3 cut(s) 234, 268, 543
DpnII GATC 3 cut(s) 232, 266, 541
DrdI GACNNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 110
FaqI GGGAC 1 cut(s) 596
FspBI CTAG 1 cut(s) 560
GlaI GCGC 1 cut(s) 98
HapII CCGG 2 cut(s) 29, 80
HhaI GCGC 1 cut(s) 99
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 1 cut(s) 226
HpaII CCGG 2 cut(s) 29, 80
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 4 cut(s) 146, 189, 230, 613
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 3 cut(s) 48, 185, 512
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4V TGCA 9 cut(s) 198, 347, 371, 395, 428, 452, 554, 569, 584
HpyF3I CTNAG 1 cut(s) 9
HspAI GCGC 1 cut(s) 97
Kzo9I GATC 3 cut(s) 232, 266, 541
LmnI GCTCC 2 cut(s) 31, 334
LpnPI CCDG 9 cut(s) 42, 86, 93, 97, 124, 195, 243, 283, 345
LweI GCATC 2 cut(s) 111, 182
MaeI CTAG 1 cut(s) 560
MaeIII GTNAC 2 cut(s) 217, 475
MalI GATC 3 cut(s) 234, 268, 543
MboI GATC 3 cut(s) 232, 266, 541
MflI RGATCY 1 cut(s) 232
MluCI AATT 3 cut(s) 135, 483, 576
MnlI CCTC 4 cut(s) 123, 142, 145, 548
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 2 cut(s) 239, 309
MspA1I CMGCKG 5 cut(s) 350, 374, 398, 431, 455
MspI CCGG 2 cut(s) 29, 80
MspR9I CCNGG 2 cut(s) 80, 112
MvaI CCWGG 1 cut(s) 112
NciI CCSGG 1 cut(s) 80
NdeII GATC 3 cut(s) 232, 266, 541
NmuCI GTSAC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 110
PspGI CCWGG 1 cut(s) 110
PsuI RGATCY 1 cut(s) 232
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SaqAI TTAA 2 cut(s) 239, 309
Sau3AI GATC 3 cut(s) 232, 266, 541
ScrFI CCNGG 2 cut(s) 80, 112
SetI ASST 3 cut(s) 10, 184, 247
SfaNI GCATC 2 cut(s) 111, 182
SmlI CTYRAG 2 cut(s) 125, 146
SmoI CTYRAG 2 cut(s) 125, 146
SpeI ACTAGT 1 cut(s) 559
Sse9I AATT 3 cut(s) 135, 483, 576
SspMI CTAG 1 cut(s) 560
StyD4I CCNGG 2 cut(s) 78, 110
TaaI ACNGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 253, 540
TasI AATT 3 cut(s) 135, 483, 576
TauI GCSGC 3 cut(s) 49, 52, 70
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 2 cut(s) 239, 309
Tru9I TTAA 2 cut(s) 239, 309
TseFI GTSAC 1 cut(s) 217
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 9 cut(s) 332, 356, 380, 413, 425, 449, 461, 554, 596
TspGWI ACGGA 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.