Rroxscaffold_2G00141840

HECT-like Ubiquitin-conjugating enzyme (E2)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015450
Physical Location & Seq
Reverse (-)
80082575 .. 80085467
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141840.1

Sequence Viewer

Length: 1710 bp
ATGAGCTTGAAGCCTGAAACCACCGGCGAAACTGGTACAAAATGGCGGTTCACATGGGAATCCCAATCCCACATCCCAACCCTCCGCCTATTCCTATTCGATTCCTCCACAACCCCCTCCACTCAATGCCACGACCTCAAGGTTCACCTCAGCCTCTCAGACTCTCTCGTCCTGCTGACCTGGACCGCCGCCGGCGCCGGCGAAGTCTCGCTCAGGGTTCCCATGCCTAGGGTTTTGCTGGACGACGAGTCGCCGGTCAGCTTCAAAGCCTTGGATGATCACATTGAGGTCAAGCTCGCCCTTCTTCTCCCTGTTGATCACTCTATTGTGCTCAACTTCGACTCTATACTCAGTTTGAGTGATATGAGGCTGAATGGAGACGAAGAGAATGGAGCGAAACCGCTCTCGGTGGACTCCGGTGAGCTCAAATTATGTAAAGAGTCTATCTTCTGGGGAGAAGTTCACTTTTATTGCAGAAGTTGCTCATATAAGTTGACTGCAAGACCCCTTAGAACTTTTGTGGAAATGCCTTCAGTCAACTGGCGAGAGGTGGCGGATAACTGGTTTGGGAATTGCTGCTGTTCGTTTGGAGAAATTAGTGAGAAGCTGGTTGCTGGATATGCAAATTCTTACACAAGTAAGATGGGTGTGTGCCTGGTGAGCTCTACAAATATTACGCTTTGCAAAGATGATCTTGTTGGATGTGAATTTCCTGACTCGGGTGTATGTGAAAGACATGATAATGAATCAGATGCCAGTGGTGAGTGTGGTCCTAATGAATCTGAACTTGCTCCTGGAAGTAATGGCGGATGTAATGTGATACTTCAGTCAGAAAATAAGGAAAAGTATATAGATGCCGATTGTAAAAGTGACGCTACTAAGGAAGAAAGTAACAGTGAGGGTGTGCCTTGTAGGGGTTCAGAATCGGATTGTTCTGTAAGATTGACATCAGTAGCAGTATGCTGTGATGATATGGAAAGTCATGTGCAAAACTATGATGGTGAAGGCTGCAGGCATCATTTGTCTGAATCTTCTACAGAGCAAAATACGGAGATTCTGAAAAATCACAAATCTTTGCTAAATGGGTTTCTTGAAAATATTTTCATGGTTAGGTCCTCCAATCTTTCAGTAGATGTTGAATGGGTTGAATTTTTCTGCCCTCAGTGTTCATCTCTCCTTGGAGCTTACCCTTGTGATAATGGTGGCAGACTTATTGATGGTGGTGTTCGATTGTTTAAATGCAGTATTGCAACTAGTCTTCCAGTTGGTGGGACAAGAGATGTTTTCAGGAAGTCTACCCTGGAGAGAATGTTTGCAAACCAGCTACTCGAATGTGCAAAAGATCAGTTATCATTTAGGACCGTGGTTAGGGACCTGAAACAAAGATCTCCAATGCTGCAAATTGTTCTCATAAATACAAATTCTTGGTCTAGCACTGGTCATTGTTTGGCCACACAGGGCAATACCAAATCAGTACCAAAGATAGATCTGCATCCTGTCATCAAGGTGCTATTTTCCGAGTGCAGCAGCAGCACTGAGTCTCAATTAAGGATGTTGGAAGATTCAATAACAAAGGATGTAGCTGAGGAGGTTTTCATGCTGACACATCAGATAGAAGAATTAATCAAGTCTCTGTCAGCAAGACGGGATATACTTCCACCTTCATACTCATCCCTCGAGGGTTTATCTTTGTCATCTATGCCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT_2

Protein Analysis

569

Amino Acids

62.83

Weight (kDa)

4.94

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT_2 PF09814 126 - 559 1.6e-19 HECT-like Ubiquitin-conjugating enzyme (E2)-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26750
fragaria_vesca FvH4_1g12740
malus_domestica MD15G1254500.v1.1
prunus_persica Prupe.7G159100_v2.0.a1 Prupe.7G159100_v2.0.a1
pyrus_communis pycom15g22360
rosa_chinensis RchiOBHm_Chr2g0100811
rosa_laevigata RLG00000017013
rosa_multiflora Rmu_sc0001659.1_g000020
rosa_roxburghii Rroxscaffold_2G00141840
rosa_rugosa Rorug02G0093000
rosa_samantha Rh2AG140600 Rh2BG145600 Rh2CG146600 Rh2DG146000
rosa_wichuraiana Rw2G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AbsI CCTCGAGG 1 cut(s) 1676
AccB1I GGYRCC 1 cut(s) 194
AccB7I CCANNNNNTGG 1 cut(s) 1268
AccBSI CCGCTC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 1295
AciI CCGC 7 cut(s) 46, 85, 186, 189, 401, 554, 807
AcoI YGGCCR 1 cut(s) 1449
AcsI RAATTY 4 cut(s) 625, 707, 1148, 1420
AcuI CTGAAG 2 cut(s) 516, 809
AcyI GRCGYC 1 cut(s) 195
AfaI GTAC 2 cut(s) 37, 1476
AfiI CCNNNNNNNGG 5 cut(s) 228, 719, 914, 1268, 1368
AgsI TTSAA 6 cut(s) 10, 265, 1094, 1139, 1148, 1566
AhdI GACNNNNNGTC 1 cut(s) 247
AhlI ACTAGT 1 cut(s) 1253
AjnI CCWGG 4 cut(s) 179, 654, 793, 1299
AluBI AGCT 9 cut(s) 6, 261, 295, 424, 607, 663, 1184, 1324, 1583
AluI AGCT 9 cut(s) 6, 261, 295, 424, 607, 663, 1184, 1324, 1583
Alw21I GWGCWC 3 cut(s) 333, 426, 665
Alw26I GTCTC 4 cut(s) 211, 372, 1545, 1635
Ama87I CYCGRG 2 cut(s) 718, 1676
AoxI GGCC 1 cut(s) 1449
ApeKI GCWGC 6 cut(s) 576, 1008, 1396, 1524, 1527, 1530
ApoI RAATTY 4 cut(s) 625, 707, 1148, 1420
ArsI GACNNNNNNTTYG 2 cut(s) 1412, 1444
AseI ATTAAT 1 cut(s) 1622
AspA2I CCTAGG 1 cut(s) 227
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 5 cut(s) 183, 770, 1113, 1359, 1372
AsuHPI GGTGA 5 cut(s) 137, 431, 670, 773, 1013
AvaI CYCGRG 2 cut(s) 718, 1676
AvaII GGWCC 5 cut(s) 183, 770, 1113, 1359, 1372
AvrII CCTAGG 1 cut(s) 227
BaeI ACNNNNGTAYC 2 cut(s) 812, 845
BalI TGGCCA 1 cut(s) 1451
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 2 cut(s) 426, 665
BbsI GAAGAC 1 cut(s) 1250
Bbv12I GWGCWC 3 cut(s) 333, 426, 665
BbvCI CCTCAGC 2 cut(s) 149, 1584
BbvI GCAGC 6 cut(s) 563, 995, 1383, 1536, 1539, 1542
BccI CCATC 3 cut(s) 637, 992, 1211
BciT130I CCWGG 4 cut(s) 181, 656, 795, 1301
BclI TGATCA 2 cut(s) 277, 316
BcoDI GTCTC 4 cut(s) 211, 372, 1545, 1635
BcuI ACTAGT 1 cut(s) 1253
BfaI CTAG 3 cut(s) 228, 1254, 1431
BfmI CTRYAG 2 cut(s) 1009, 1035
BfoI RGCGCY 1 cut(s) 198
BglII AGATCT 2 cut(s) 1385, 1486
BisI GCNGC 7 cut(s) 189, 577, 1009, 1397, 1525, 1528, 1531
BlnI CCTAGG 1 cut(s) 227
BlsI GCNGC 7 cut(s) 190, 578, 1010, 1398, 1526, 1529, 1532
Bme1390I CCNGG 4 cut(s) 181, 656, 795, 1301
Bme18I GGWCC 5 cut(s) 183, 770, 1113, 1359, 1372
BmeRI GACNNNNNGTC 1 cut(s) 247
BmeT110I CYCGRG 2 cut(s) 718, 1676
BmgT120I GGNCC 5 cut(s) 183, 770, 1113, 1359, 1372
BmiI GGNNCC 3 cut(s) 196, 219, 1373
BmrFI CCNGG 4 cut(s) 181, 656, 795, 1301
BmsI GCATC 4 cut(s) 742, 844, 1024, 1501
BpiI GAAGAC 1 cut(s) 1250
BpmI CTGGAG 1 cut(s) 1322
Bpu10I CCTNAGC 3 cut(s) 149, 212, 1584
BpuEI CTTGAG 1 cut(s) 122
BsaBI GATNNNNATC 2 cut(s) 946, 1491
BsaHI GRCGYC 1 cut(s) 195
BsaJI CCNNGG 5 cut(s) 227, 270, 1177, 1299, 1362
BsaWI WCCGGW 1 cut(s) 416
BsaXI ACNNNNNCTCC 2 cut(s) 1295, 1325
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 719, 914, 1268, 1368
Bse118I RCCGGY 4 cut(s) 23, 191, 197, 253
Bse1I ACTGG 6 cut(s) 37, 545, 566, 756, 1262, 1441
Bse8I GATNNNNATC 2 cut(s) 946, 1491
BseBI CCWGG 4 cut(s) 181, 656, 795, 1301
BseDI CCNNGG 5 cut(s) 227, 270, 1177, 1299, 1362
BseGI GGATG 8 cut(s) 72, 280, 707, 815, 1492, 1557, 1582, 1670
BseJI GATNNNNATC 2 cut(s) 946, 1491
BseLI CCNNNNNNNGG 5 cut(s) 228, 719, 914, 1268, 1368
BseMII CTCAG 7 cut(s) 163, 171, 226, 364, 1175, 1527, 1575
BseNI ACTGG 6 cut(s) 37, 545, 566, 756, 1262, 1441
BseRI GAGGAG 1 cut(s) 1601
BseXI GCAGC 6 cut(s) 563, 995, 1383, 1536, 1539, 1542
BsgI GTGCAG 1 cut(s) 1543
BshFI GGCC 1 cut(s) 1451
BshNI GGYRCC 1 cut(s) 194
BsiHKAI GWGCWC 3 cut(s) 333, 426, 665
BsiHKCI CYCGRG 2 cut(s) 718, 1676
BsiSI CCGG 5 cut(s) 24, 192, 198, 254, 417
BslFI GGGAC 2 cut(s) 1285, 1385
BslI CCNNNNNNNGG 5 cut(s) 228, 719, 914, 1268, 1368
BsmAI GTCTC 4 cut(s) 211, 372, 1545, 1635
BsmBI CGTCTC 1 cut(s) 372
BsmFI GGGAC 2 cut(s) 1285, 1385
BsnI GGCC 1 cut(s) 1451
BsoBI CYCGRG 2 cut(s) 718, 1676
Bsp1286I GDGCHC 3 cut(s) 333, 426, 665
Bsp143I GATC 6 cut(s) 277, 316, 691, 1342, 1385, 1486
BspACI CCGC 7 cut(s) 46, 85, 186, 189, 401, 554, 807
BspANI GGCC 1 cut(s) 1451
BspCNI CTCAG 7 cut(s) 162, 170, 225, 363, 1174, 1528, 1576
BspLI GGNNCC 3 cut(s) 196, 219, 1373
BspMAI CTGCAG 1 cut(s) 1013
BspT107I GGYRCC 1 cut(s) 194
BsrBI CCGCTC 1 cut(s) 403
BsrFI RCCGGY 4 cut(s) 23, 191, 197, 253
BsrI ACTGG 6 cut(s) 37, 545, 566, 756, 1262, 1441
BssAI RCCGGY 4 cut(s) 23, 191, 197, 253
BssECI CCNNGG 5 cut(s) 227, 270, 1177, 1299, 1362
BssMI GATC 6 cut(s) 277, 316, 691, 1342, 1385, 1486
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 3 cut(s) 227, 270, 1177
Bst2UI CCWGG 4 cut(s) 181, 656, 795, 1301
Bst4CI ACNGT 2 cut(s) 896, 1363
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 195
BstAPI GCANNNNNTGC 1 cut(s) 480
BstC8I GCNNGC 4 cut(s) 193, 199, 297, 1013
BstDEI CTNAG 9 cut(s) 149, 157, 212, 350, 509, 879, 1161, 1536, 1584
BstDSI CCRYGG 1 cut(s) 1362
BstF5I GGATG 8 cut(s) 72, 280, 707, 815, 1492, 1557, 1582, 1670
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 6 cut(s) 280, 319, 694, 1345, 1388, 1489
BstMAI GTCTC 4 cut(s) 211, 372, 1545, 1635
BstMBI GATC 6 cut(s) 277, 316, 691, 1342, 1385, 1486
BstMWI GCNNNNNNNGC 5 cut(s) 194, 480, 620, 660, 1530
BstNI CCWGG 4 cut(s) 181, 656, 795, 1301
BstSCI CCNGG 4 cut(s) 179, 654, 793, 1299
BstSFI CTRYAG 2 cut(s) 1009, 1035
BstV1I GCAGC 6 cut(s) 563, 995, 1383, 1536, 1539, 1542
BstV2I GAAGAC 1 cut(s) 1250
BstX2I RGATCY 2 cut(s) 1385, 1486
BstYI RGATCY 2 cut(s) 1385, 1486
BsuRI GGCC 1 cut(s) 1451
BtgI CCRYGG 1 cut(s) 1362
BtsCI GGATG 8 cut(s) 72, 280, 707, 815, 1492, 1557, 1582, 1670
BtsIMutI CAGTG 5 cut(s) 763, 901, 1169, 1434, 1533
Cac8I GCNNGC 4 cut(s) 193, 199, 297, 1013
CfoI GCGC 1 cut(s) 197
Cfr10I RCCGGY 4 cut(s) 23, 191, 197, 253
Cfr13I GGNCC 5 cut(s) 183, 770, 1113, 1359, 1372
CseI GACGC 1 cut(s) 881
Csp6I GTAC 2 cut(s) 36, 1475
CviAII CATG 6 cut(s) 54, 223, 737, 983, 1105, 1597
CviQI GTAC 2 cut(s) 36, 1475
DdeI CTNAG 9 cut(s) 149, 157, 212, 350, 509, 879, 1161, 1536, 1584
DinI GGCGCC 1 cut(s) 196
DpnI GATC 6 cut(s) 279, 318, 693, 1344, 1387, 1488
DpnII GATC 6 cut(s) 277, 316, 691, 1342, 1385, 1486
DraI TTTAAA 1 cut(s) 1237
DrdI GACNNNNNNGTC 1 cut(s) 167
DriI GACNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 1 cut(s) 1449
Eam1104I CTCTTC 1 cut(s) 378
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 3 cut(s) 74, 569, 822
Ecl136II GAGCTC 2 cut(s) 424, 663
Eco130I CCWWGG 3 cut(s) 227, 270, 1177
Eco24I GRGCYC 2 cut(s) 426, 665
Eco47I GGWCC 5 cut(s) 183, 770, 1113, 1359, 1372
Eco53kI GAGCTC 2 cut(s) 424, 663
Eco57I CTGAAG 2 cut(s) 516, 809
Eco88I CYCGRG 2 cut(s) 718, 1676
EcoICRI GAGCTC 2 cut(s) 424, 663
EcoO109I RGGNCCY 2 cut(s) 1113, 1372
EcoRII CCWGG 4 cut(s) 179, 654, 793, 1299
EcoT14I CCWWGG 3 cut(s) 227, 270, 1177
EcoT38I GRGCYC 2 cut(s) 426, 665
EgeI GGCGCC 1 cut(s) 196
EheI GGCGCC 1 cut(s) 196
ErhI CCWWGG 3 cut(s) 227, 270, 1177
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 6 cut(s) 57, 226, 740, 986, 1108, 1600
FalI AAGNNNNNCTT 2 cut(s) 678, 710
FaqI GGGAC 2 cut(s) 1285, 1385
FatI CATG 6 cut(s) 53, 222, 736, 982, 1104, 1596
FbaI TGATCA 2 cut(s) 277, 316
FblI GTMKAC 1 cut(s) 1295
Fnu4HI GCNGC 7 cut(s) 189, 577, 1009, 1397, 1525, 1528, 1531
FokI GGATG 8 cut(s) 59, 287, 714, 822, 1479, 1564, 1589, 1657
FriOI GRGCYC 2 cut(s) 426, 665
Fsp4HI GCNGC 7 cut(s) 189, 577, 1009, 1397, 1525, 1528, 1531
FspBI CTAG 3 cut(s) 228, 1254, 1431
GlaI GCGC 1 cut(s) 196
GluI GCNGC 7 cut(s) 189, 577, 1009, 1397, 1525, 1528, 1531
GsuI CTGGAG 1 cut(s) 1322
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 1 cut(s) 1451
HapII CCGG 5 cut(s) 24, 192, 198, 254, 417
HgaI GACGC 1 cut(s) 881
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 6 cut(s) 57, 226, 740, 986, 1108, 1600
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HincII GTYRAC 2 cut(s) 495, 538
HindII GTYRAC 2 cut(s) 495, 538
HpaII CCGG 5 cut(s) 24, 192, 198, 254, 417
HphI GGTGA 5 cut(s) 137, 431, 670, 773, 1013
Hpy166II GTNNAC 7 cut(s) 51, 145, 412, 463, 495, 538, 1296
Hpy188III TCNNGA 3 cut(s) 713, 1091, 1288
Hpy8I GTNNAC 7 cut(s) 51, 145, 412, 463, 495, 538, 1296
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 4 cut(s) 311, 540, 998, 1671
HpyCH4III ACNGT 2 cut(s) 896, 1363
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 480, 620, 660, 1530
HpyF3I CTNAG 9 cut(s) 149, 157, 212, 350, 509, 879, 1161, 1536, 1584
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 6 cut(s) 57, 226, 740, 986, 1108, 1600
HspAI GCGC 1 cut(s) 195
KasI GGCGCC 1 cut(s) 194
KroI GCCGGC 2 cut(s) 191, 197
KroNI GCCGGC 2 cut(s) 193, 199
Ksp22I TGATCA 2 cut(s) 277, 316
Kzo9I GATC 6 cut(s) 277, 316, 691, 1342, 1385, 1486
LmnI GCTCC 3 cut(s) 392, 796, 1181
Lsp1109I GCAGC 6 cut(s) 563, 995, 1383, 1536, 1539, 1542
LweI GCATC 4 cut(s) 742, 844, 1024, 1501
MaeI CTAG 3 cut(s) 228, 1254, 1431
MaeIII GTNAC 2 cut(s) 869, 890
MalI GATC 6 cut(s) 279, 318, 693, 1344, 1387, 1488
MbiI CCGCTC 1 cut(s) 403
MboI GATC 6 cut(s) 277, 316, 691, 1342, 1385, 1486
MboII GAAGA 8 cut(s) 296, 395, 439, 896, 1023, 1250, 1571, 1628
MflI RGATCY 2 cut(s) 1385, 1486
MhlI GDGCHC 3 cut(s) 333, 426, 665
MlsI TGGCCA 1 cut(s) 1451
MluNI TGGCCA 1 cut(s) 1451
Mly113I GGCGCC 1 cut(s) 195
MlyI GAGTC 7 cut(s) 155, 257, 335, 407, 449, 710, 1547
MmeI TCCRAC 2 cut(s) 679, 1536
Mox20I TGGCCA 1 cut(s) 1451
MreI CGCCGGCG 2 cut(s) 191, 197
MroNI GCCGGC 2 cut(s) 191, 197
MscI TGGCCA 1 cut(s) 1451
MseI TTAA 3 cut(s) 1236, 1547, 1622
MslI CAYNNNNRTG 2 cut(s) 741, 1505
Msp20I TGGCCA 1 cut(s) 1451
MspI CCGG 5 cut(s) 24, 192, 198, 254, 417
MspR9I CCNGG 4 cut(s) 181, 656, 795, 1301
MvaI CCWGG 4 cut(s) 181, 656, 795, 1301
MwoI GCNNNNNNNGC 5 cut(s) 194, 480, 620, 660, 1530
NaeI GCCGGC 2 cut(s) 193, 199
NarI GGCGCC 1 cut(s) 195
NdeII GATC 6 cut(s) 277, 316, 691, 1342, 1385, 1486
NgoMIV GCCGGC 2 cut(s) 191, 197
NlaIII CATG 6 cut(s) 57, 226, 740, 986, 1108, 1600
NlaIV GGNNCC 3 cut(s) 196, 219, 1373
NmuCI GTSAC 1 cut(s) 869
PaeR7I CTCGAG 1 cut(s) 1676
PdiI GCCGGC 2 cut(s) 193, 199
PfeI GAWTC 8 cut(s) 59, 101, 746, 779, 923, 1028, 1054, 1562
PflMI CCANNNNNTGG 1 cut(s) 1268
PfoI TCCNGGA 1 cut(s) 793
PkrI GCNGC 7 cut(s) 190, 578, 1010, 1398, 1526, 1529, 1532
PleI GAGTC 7 cut(s) 155, 256, 335, 407, 448, 710, 1546
PluTI GGCGCC 1 cut(s) 198
PpsI GAGTC 7 cut(s) 155, 256, 335, 407, 448, 710, 1546
PpuMI RGGWCCY 2 cut(s) 1113, 1372
PshBI ATTAAT 1 cut(s) 1622
Psp124BI GAGCTC 2 cut(s) 426, 665
Psp5II RGGWCCY 2 cut(s) 1113, 1372
Psp6I CCWGG 4 cut(s) 179, 654, 793, 1299
PspGI CCWGG 4 cut(s) 179, 654, 793, 1299
PspN4I GGNNCC 3 cut(s) 196, 219, 1373
PspPI GGNCC 5 cut(s) 183, 770, 1113, 1359, 1372
PspPPI RGGWCCY 2 cut(s) 1113, 1372
PspXI VCTCGAGB 1 cut(s) 1676
PstI CTGCAG 1 cut(s) 1013
PsuI RGATCY 2 cut(s) 1385, 1486
RsaI GTAC 2 cut(s) 37, 1476
RsaNI GTAC 2 cut(s) 36, 1475
RseI CAYNNNNRTG 2 cut(s) 741, 1505
SacI GAGCTC 2 cut(s) 426, 665
SaqAI TTAA 3 cut(s) 1236, 1547, 1622
SatI GCNGC 7 cut(s) 189, 577, 1009, 1397, 1525, 1528, 1531
Sau3AI GATC 6 cut(s) 277, 316, 691, 1342, 1385, 1486
Sau96I GGNCC 5 cut(s) 183, 770, 1113, 1359, 1372
SchI GAGTC 7 cut(s) 155, 257, 335, 407, 449, 710, 1547
ScrFI CCNGG 4 cut(s) 181, 656, 795, 1301
SduI GDGCHC 3 cut(s) 333, 426, 665
SfaNI GCATC 4 cut(s) 742, 844, 1024, 1501
SfcI CTRYAG 2 cut(s) 1009, 1035
SfoI GGCGCC 1 cut(s) 196
Sfr274I CTCGAG 1 cut(s) 1676
SgrAI CRCCGGYG 3 cut(s) 23, 191, 197
SinI GGWCC 5 cut(s) 183, 770, 1113, 1359, 1372
SlaI CTCGAG 1 cut(s) 1676
SmiMI CAYNNNNRTG 2 cut(s) 741, 1505
SmlI CTYRAG 2 cut(s) 137, 1676
SmoI CTYRAG 2 cut(s) 137, 1676
SpeI ACTAGT 1 cut(s) 1253
SsiI CCGC 7 cut(s) 46, 85, 186, 189, 401, 554, 807
SspDI GGCGCC 1 cut(s) 194
SspI AATATT 2 cut(s) 673, 1099
SspMI CTAG 3 cut(s) 228, 1254, 1431
SstI GAGCTC 2 cut(s) 426, 665
StyD4I CCNGG 4 cut(s) 179, 654, 793, 1299
StyI CCWWGG 3 cut(s) 227, 270, 1177
TaaI ACNGT 2 cut(s) 896, 1363
TaqI TCGA 5 cut(s) 99, 339, 1228, 1329, 1677
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 8 cut(s) 59, 101, 746, 779, 923, 1028, 1054, 1562
Tru1I TTAA 3 cut(s) 1236, 1547, 1622
Tru9I TTAA 3 cut(s) 1236, 1547, 1622
TscAI CASTG 5 cut(s) 763, 901, 1169, 1441, 1540
TseFI GTSAC 1 cut(s) 869
TseI GCWGC 6 cut(s) 576, 1008, 1396, 1524, 1527, 1530
Tsp45I GTSAC 1 cut(s) 869
TspDTI ATGAA 6 cut(s) 759, 792, 1093, 1158, 1585, 1653
TspGWI ACGGA 1 cut(s) 1064
TspRI CASTG 5 cut(s) 763, 901, 1169, 1441, 1540
Van91I CCANNNNNTGG 1 cut(s) 1268
VpaK11BI GGWCC 5 cut(s) 183, 770, 1113, 1359, 1372
VspI ATTAAT 1 cut(s) 1622
XapI RAATTY 4 cut(s) 625, 707, 1148, 1420
XhoI CTCGAG 1 cut(s) 1676
XmaJI CCTAGG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 1295
XspI CTAG 3 cut(s) 228, 1254, 1431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.