Rh2BG145600

HECT-like Ubiquitin-conjugating enzyme (E2)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015450
Physical Location & Seq
Forward (+)
12514370 .. 12516026
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG145600.1

Sequence Viewer

Length: 1308 bp
ATGAGCCTTAAGCCTGAAACCACCGGCGAAACTGGTATAAAATGGCGGTTCACATGGGAATCCCAATCCCACATCCCAACCCTCCGCCTATTCCTATTCGATTCCTCCACCACCCCCTCCACTCAATGCCACGACCTCAAGGTCCACCTCAGCCTCTCAGACTCTCTCGTCCTGCTGACCTGGACCGCCGCCGGCGCCGGCGAAGTCTCGCTCAGGGTTCCCATGCCTAGGGTTTTGCTGGACGACGAGTCGCCGGTCAGCTTCAAAGCCTTGGATGACCACATTGAGGTCAAGCTCGCCCTCCTTCTCCCTGTTGATCACTCTATTGTGCTCAACTTCGACTCTATACTCAGTTTGAGTGAGATGAGGTTGAATGGAGACGAAGAGAATGGAGCGAAGCCGCTCTCGGTGGACTCCGATGTAAAGAGTCTATCTTCTGGGGGAGAAGTTCACTTTTATTGCAGAAGTTGCTCATATAAGTTGACTGCAAGACCCCTTAGAACTTTTGTGGAAATGCCTTCAGTCAACTGGCGAGAGGTGGCGGATAATTGGTTTGGGAATTGCTGCTGTTCGTTTGGAGAAGTTAGTGAGAAGCTGGTTGCTGGATATGCAAATTCTTACACAAGTAAGATTGGTGTGTGCCTGGTGAGCTCTACAAATATTACGCTTTGCAAAGATGATCTTGTTGATTGTGAGTTTCTCGAATCGGGTGTATGTGAAAGACAGGATAATGAATCAGATGCCAGTGGTGAGTGTGGTCCTAATGAATCTGAACTTGCTCCTGGAAGTAATGGCGGATGTAATGAGATACTTAAGTCAGAAAATAAGGAAAAGTATATAGATGCCGATTGTAAAAGTGACGCTACTAAGGAAGAAAGTAACAGTGAGGGTGTGCCTCGTAGGGGTTCAGAATCGGATTGTTCTGTAAGATTGACATCAGTAGCAGTATGCTGTGATGATATGGAAAGTCATGTGCAAAACTATGATGGTGAAGGCTGCAGGCATCATTTGTCTGAATCTTCTACAGAGCAAAATACAGAGATTCTGAAAAATCACAAATCTTTGCTAAATGGGTTTCTTGAAAATATTTTCATGGTTAGGTCCTCCAATCTTTCAGTAGATGTTGAATGGGTCGAATTCTTCTGCCCTCAGTGTTCATCTCTCCTTGGAGCTTACCCTTGTGATAATGGTGGCAGACTTATTGATGGTGGTGTTCGATTGTTTAAATGCAGTATTGCAACTGGTCTTCCAGTTGGTGGGACAAGAGACGTTTTCAGGTTGGCTTATAATGATCTACAATTCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT_2

Protein Analysis

435

Amino Acids

47.78

Weight (kDa)

4.7

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT_2 PF09814 45 - 245 9.3e-08 HECT-like Ubiquitin-conjugating enzyme (E2)-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26750
fragaria_vesca FvH4_1g12740
malus_domestica MD15G1254500.v1.1
prunus_persica Prupe.7G159100_v2.0.a1 Prupe.7G159100_v2.0.a1
pyrus_communis pycom15g22360
rosa_chinensis RchiOBHm_Chr2g0100811
rosa_laevigata RLG00000017013
rosa_multiflora Rmu_sc0001659.1_g000020
rosa_roxburghii Rroxscaffold_2G00141840
rosa_rugosa Rorug02G0093000
rosa_samantha Rh2AG140600 Rh2BG145600 Rh2CG146600 Rh2DG146000
rosa_wichuraiana Rw2G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1287
AasI GACNNNNNNGTC 2 cut(s) 140, 167
AccB1I GGYRCC 1 cut(s) 194
AccB7I CCANNNNNTGG 1 cut(s) 1256
AccBSI CCGCTC 1 cut(s) 403
AciI CCGC 7 cut(s) 46, 85, 186, 189, 401, 542, 795
AcsI RAATTY 2 cut(s) 613, 1136
AcuI CTGAAG 1 cut(s) 504
AcyI GRCGYC 1 cut(s) 195
AfiI CCNNNNNNNGG 4 cut(s) 228, 286, 902, 1256
AflII CTTAAG 2 cut(s) 8, 812
AgsI TTSAA 4 cut(s) 265, 373, 1082, 1127
AhdI GACNNNNNGTC 1 cut(s) 247
AjnI CCWGG 3 cut(s) 179, 642, 781
AluBI AGCT 5 cut(s) 261, 295, 595, 651, 1172
AluI AGCT 5 cut(s) 261, 295, 595, 651, 1172
Alw21I GWGCWC 2 cut(s) 333, 653
Alw26I GTCTC 3 cut(s) 211, 372, 1260
ApeKI GCWGC 2 cut(s) 564, 996
ApoI RAATTY 2 cut(s) 613, 1136
AspA2I CCTAGG 1 cut(s) 227
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 4 cut(s) 142, 183, 758, 1101
AsuHPI GGTGA 3 cut(s) 658, 761, 1001
AvaII GGWCC 4 cut(s) 142, 183, 758, 1101
AvrII CCTAGG 1 cut(s) 227
BaeI ACNNNNGTAYC 2 cut(s) 800, 833
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 653
BbsI GAAGAC 1 cut(s) 1238
Bbv12I GWGCWC 2 cut(s) 333, 653
BbvCI CCTCAGC 1 cut(s) 149
BbvI GCAGC 2 cut(s) 551, 983
BccI CCATC 2 cut(s) 980, 1199
BciT130I CCWGG 3 cut(s) 181, 644, 783
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 3 cut(s) 211, 372, 1260
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 2 cut(s) 997, 1023
BfoI RGCGCY 1 cut(s) 198
BfrI CTTAAG 2 cut(s) 8, 812
BisI GCNGC 4 cut(s) 189, 401, 565, 997
BlnI CCTAGG 1 cut(s) 227
BlsI GCNGC 4 cut(s) 190, 402, 566, 998
Bme1390I CCNGG 3 cut(s) 181, 644, 783
Bme18I GGWCC 4 cut(s) 142, 183, 758, 1101
BmeRI GACNNNNNGTC 1 cut(s) 247
BmgT120I GGNCC 4 cut(s) 142, 183, 758, 1101
BmiI GGNNCC 2 cut(s) 196, 219
BmrFI CCNGG 3 cut(s) 181, 644, 783
BmsI GCATC 3 cut(s) 730, 832, 1012
BpiI GAAGAC 1 cut(s) 1238
Bpu10I CCTNAGC 2 cut(s) 149, 212
BpuEI CTTGAG 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 934
BsaHI GRCGYC 1 cut(s) 195
BsaJI CCNNGG 3 cut(s) 227, 270, 1165
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 286, 902, 1256
Bse118I RCCGGY 4 cut(s) 23, 191, 197, 253
Bse1I ACTGG 5 cut(s) 37, 533, 744, 1246, 1250
Bse8I GATNNNNATC 1 cut(s) 934
BseBI CCWGG 3 cut(s) 181, 644, 783
BseDI CCNNGG 3 cut(s) 227, 270, 1165
BseGI GGATG 3 cut(s) 72, 280, 803
BseJI GATNNNNATC 1 cut(s) 934
BseLI CCNNNNNNNGG 4 cut(s) 228, 286, 902, 1256
BseMII CTCAG 5 cut(s) 163, 171, 226, 364, 1163
BseNI ACTGG 5 cut(s) 37, 533, 744, 1246, 1250
BseXI GCAGC 2 cut(s) 551, 983
BshNI GGYRCC 1 cut(s) 194
BsiHKAI GWGCWC 2 cut(s) 333, 653
BsiSI CCGG 4 cut(s) 24, 192, 198, 254
BslFI GGGAC 1 cut(s) 1273
BslI CCNNNNNNNGG 4 cut(s) 228, 286, 902, 1256
BsmAI GTCTC 3 cut(s) 211, 372, 1260
BsmBI CGTCTC 2 cut(s) 372, 1260
BsmFI GGGAC 1 cut(s) 1273
Bsp1286I GDGCHC 2 cut(s) 333, 653
Bsp143I GATC 3 cut(s) 316, 679, 1291
BspACI CCGC 7 cut(s) 46, 85, 186, 189, 401, 542, 795
BspCNI CTCAG 5 cut(s) 162, 170, 225, 363, 1162
BspLI GGNNCC 2 cut(s) 196, 219
BspMAI CTGCAG 1 cut(s) 1001
BspT107I GGYRCC 1 cut(s) 194
BspTI CTTAAG 2 cut(s) 8, 812
BsrBI CCGCTC 1 cut(s) 403
BsrFI RCCGGY 4 cut(s) 23, 191, 197, 253
BsrI ACTGG 5 cut(s) 37, 533, 744, 1246, 1250
BssAI RCCGGY 4 cut(s) 23, 191, 197, 253
BssECI CCNNGG 3 cut(s) 227, 270, 1165
BssMI GATC 3 cut(s) 316, 679, 1291
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 3 cut(s) 227, 270, 1165
Bst2UI CCWGG 3 cut(s) 181, 644, 783
Bst4CI ACNGT 1 cut(s) 884
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 195
BstAFI CTTAAG 2 cut(s) 8, 812
BstAPI GCANNNNNTGC 1 cut(s) 468
BstC8I GCNNGC 4 cut(s) 193, 199, 297, 1001
BstDEI CTNAG 7 cut(s) 149, 157, 212, 350, 497, 867, 1149
BstF5I GGATG 3 cut(s) 72, 280, 803
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 3 cut(s) 319, 682, 1294
BstMAI GTCTC 3 cut(s) 211, 372, 1260
BstMBI GATC 3 cut(s) 316, 679, 1291
BstMWI GCNNNNNNNGC 4 cut(s) 194, 468, 608, 648
BstNI CCWGG 3 cut(s) 181, 644, 783
BstSCI CCNGG 3 cut(s) 179, 642, 781
BstSFI CTRYAG 2 cut(s) 997, 1023
BstV1I GCAGC 2 cut(s) 551, 983
BstV2I GAAGAC 1 cut(s) 1238
BtsCI GGATG 3 cut(s) 72, 280, 803
BtsIMutI CAGTG 3 cut(s) 751, 889, 1157
Cac8I GCNNGC 4 cut(s) 193, 199, 297, 1001
CfoI GCGC 1 cut(s) 197
Cfr10I RCCGGY 4 cut(s) 23, 191, 197, 253
Cfr13I GGNCC 4 cut(s) 142, 183, 758, 1101
CseI GACGC 1 cut(s) 869
CviAII CATG 4 cut(s) 54, 223, 971, 1093
DdeI CTNAG 7 cut(s) 149, 157, 212, 350, 497, 867, 1149
DinI GGCGCC 1 cut(s) 196
DpnI GATC 3 cut(s) 318, 681, 1293
DpnII GATC 3 cut(s) 316, 679, 1291
DraI TTTAAA 1 cut(s) 1225
DrdI GACNNNNNNGTC 2 cut(s) 140, 167
DriI GACNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 2 cut(s) 140, 167
Eam1104I CTCTTC 1 cut(s) 378
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 3 cut(s) 74, 557, 810
Ecl136II GAGCTC 1 cut(s) 651
Eco130I CCWWGG 3 cut(s) 227, 270, 1165
Eco24I GRGCYC 1 cut(s) 653
Eco47I GGWCC 4 cut(s) 142, 183, 758, 1101
Eco53kI GAGCTC 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 504
EcoICRI GAGCTC 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 1101
EcoRI GAATTC 1 cut(s) 1136
EcoRII CCWGG 3 cut(s) 179, 642, 781
EcoT14I CCWWGG 3 cut(s) 227, 270, 1165
EcoT38I GRGCYC 1 cut(s) 653
EgeI GGCGCC 1 cut(s) 196
EheI GGCGCC 1 cut(s) 196
ErhI CCWWGG 3 cut(s) 227, 270, 1165
Esp3I CGTCTC 2 cut(s) 372, 1260
FaeI CATG 4 cut(s) 57, 226, 974, 1096
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FaqI GGGAC 1 cut(s) 1273
FatI CATG 4 cut(s) 53, 222, 970, 1092
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 4 cut(s) 189, 401, 565, 997
FokI GGATG 3 cut(s) 59, 287, 810
FriOI GRGCYC 1 cut(s) 653
Fsp4HI GCNGC 4 cut(s) 189, 401, 565, 997
FspBI CTAG 1 cut(s) 228
GlaI GCGC 1 cut(s) 196
GluI GCNGC 4 cut(s) 189, 401, 565, 997
HaeII RGCGCY 1 cut(s) 198
HapII CCGG 4 cut(s) 24, 192, 198, 254
HgaI GACGC 1 cut(s) 869
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 4 cut(s) 57, 226, 974, 1096
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HincII GTYRAC 2 cut(s) 483, 526
HindII GTYRAC 2 cut(s) 483, 526
HpaII CCGG 4 cut(s) 24, 192, 198, 254
HphI GGTGA 3 cut(s) 658, 761, 1001
Hpy166II GTNNAC 6 cut(s) 51, 145, 412, 451, 483, 526
Hpy188I TCNGA 9 cut(s) 160, 418, 739, 772, 820, 910, 916, 1015, 1047
Hpy188III TCNNGA 2 cut(s) 701, 1079
Hpy8I GTNNAC 6 cut(s) 51, 145, 412, 451, 483, 526
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 314, 528, 986
HpyCH4III ACNGT 1 cut(s) 884
HpyCH4IV ACGT 1 cut(s) 1269
HpyCH4V TGCA 8 cut(s) 462, 488, 611, 672, 976, 999, 1230, 1238
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 468, 608, 648
HpyF3I CTNAG 7 cut(s) 149, 157, 212, 350, 497, 867, 1149
HpySE526I ACGT 1 cut(s) 1269
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 4 cut(s) 57, 226, 974, 1096
HspAI GCGC 1 cut(s) 195
KasI GGCGCC 1 cut(s) 194
KroI GCCGGC 2 cut(s) 191, 197
KroNI GCCGGC 2 cut(s) 193, 199
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 3 cut(s) 316, 679, 1291
LmnI GCTCC 3 cut(s) 392, 784, 1169
Lsp1109I GCAGC 2 cut(s) 551, 983
LweI GCATC 3 cut(s) 730, 832, 1012
MaeI CTAG 1 cut(s) 228
MaeII ACGT 1 cut(s) 1269
MaeIII GTNAC 2 cut(s) 857, 878
MalI GATC 3 cut(s) 318, 681, 1293
MbiI CCGCTC 1 cut(s) 403
MboI GATC 3 cut(s) 316, 679, 1291
MboII GAAGA 6 cut(s) 395, 426, 884, 1011, 1132, 1238
MhlI GDGCHC 2 cut(s) 333, 653
MluCI AATT 5 cut(s) 547, 559, 613, 1136, 1298
Mly113I GGCGCC 1 cut(s) 195
MlyI GAGTC 5 cut(s) 155, 257, 335, 407, 436
MreI CGCCGGCG 2 cut(s) 191, 197
MroNI GCCGGC 2 cut(s) 191, 197
MseI TTAA 3 cut(s) 9, 813, 1224
MspCI CTTAAG 2 cut(s) 8, 812
MspI CCGG 4 cut(s) 24, 192, 198, 254
MspR9I CCNGG 3 cut(s) 181, 644, 783
MvaI CCWGG 3 cut(s) 181, 644, 783
MwoI GCNNNNNNNGC 4 cut(s) 194, 468, 608, 648
NaeI GCCGGC 2 cut(s) 193, 199
NarI GGCGCC 1 cut(s) 195
NdeII GATC 3 cut(s) 316, 679, 1291
NgoMIV GCCGGC 2 cut(s) 191, 197
NlaIII CATG 4 cut(s) 57, 226, 974, 1096
NlaIV GGNNCC 2 cut(s) 196, 219
NmuCI GTSAC 1 cut(s) 857
PdiI GCCGGC 2 cut(s) 193, 199
PfeI GAWTC 8 cut(s) 59, 101, 704, 734, 767, 911, 1016, 1042
PflMI CCANNNNNTGG 1 cut(s) 1256
PfoI TCCNGGA 1 cut(s) 781
PkrI GCNGC 4 cut(s) 190, 402, 566, 998
PleI GAGTC 5 cut(s) 155, 256, 335, 407, 435
PluTI GGCGCC 1 cut(s) 198
PpsI GAGTC 5 cut(s) 155, 256, 335, 407, 435
PpuMI RGGWCCY 1 cut(s) 1101
PsiI TTATAA 1 cut(s) 1287
Psp124BI GAGCTC 1 cut(s) 653
Psp5II RGGWCCY 1 cut(s) 1101
Psp6I CCWGG 3 cut(s) 179, 642, 781
PspGI CCWGG 3 cut(s) 179, 642, 781
PspN4I GGNNCC 2 cut(s) 196, 219
PspPI GGNCC 4 cut(s) 142, 183, 758, 1101
PspPPI RGGWCCY 1 cut(s) 1101
PstI CTGCAG 1 cut(s) 1001
SacI GAGCTC 1 cut(s) 653
SaqAI TTAA 3 cut(s) 9, 813, 1224
SatI GCNGC 4 cut(s) 189, 401, 565, 997
Sau3AI GATC 3 cut(s) 316, 679, 1291
Sau96I GGNCC 4 cut(s) 142, 183, 758, 1101
SchI GAGTC 5 cut(s) 155, 257, 335, 407, 436
ScrFI CCNGG 3 cut(s) 181, 644, 783
SduI GDGCHC 2 cut(s) 333, 653
SfaNI GCATC 3 cut(s) 730, 832, 1012
SfcI CTRYAG 2 cut(s) 997, 1023
SfoI GGCGCC 1 cut(s) 196
SgrAI CRCCGGYG 3 cut(s) 23, 191, 197
SinI GGWCC 4 cut(s) 142, 183, 758, 1101
SmlI CTYRAG 3 cut(s) 8, 137, 812
SmoI CTYRAG 3 cut(s) 8, 137, 812
Sse9I AATT 5 cut(s) 547, 559, 613, 1136, 1298
SsiI CCGC 7 cut(s) 46, 85, 186, 189, 401, 542, 795
SspDI GGCGCC 1 cut(s) 194
SspI AATATT 2 cut(s) 661, 1087
SspMI CTAG 1 cut(s) 228
SstI GAGCTC 1 cut(s) 653
StyD4I CCNGG 3 cut(s) 179, 642, 781
StyI CCWWGG 3 cut(s) 227, 270, 1165
TaaI ACNGT 1 cut(s) 884
TaiI ACGT 1 cut(s) 1272
TaqI TCGA 5 cut(s) 99, 339, 702, 1134, 1216
TasI AATT 5 cut(s) 547, 559, 613, 1136, 1298
TauI GCSGC 2 cut(s) 191, 403
TfiI GAWTC 8 cut(s) 59, 101, 704, 734, 767, 911, 1016, 1042
Tru1I TTAA 3 cut(s) 9, 813, 1224
Tru9I TTAA 3 cut(s) 9, 813, 1224
TscAI CASTG 3 cut(s) 751, 889, 1157
TseFI GTSAC 1 cut(s) 857
TseI GCWGC 2 cut(s) 564, 996
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 4 cut(s) 747, 780, 1081, 1146
TspRI CASTG 3 cut(s) 751, 889, 1157
Van91I CCANNNNNTGG 1 cut(s) 1256
Vha464I CTTAAG 2 cut(s) 8, 812
VpaK11BI GGWCC 4 cut(s) 142, 183, 758, 1101
XapI RAATTY 2 cut(s) 613, 1136
XmaJI CCTAGG 1 cut(s) 227
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.