RLG00000017013

HECT-like Ubiquitin-conjugating enzyme (E2)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015450
Physical Location & Seq
Forward (+)
12101811 .. 12104739
2929 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017013

Sequence Viewer

Length: 1470 bp
ATGCCTAGGGTTTTGCTGGACGACGAGTCGCCGGTCAGCTTCAAAGCCTTGGATGACCACATTGAGGTCAAGCTCGCCCTTCTTCTCCCTGTTGATCACTCTATTGTGCTCAACTTCGACTCTATACTCAGTTTGAGTGAGATGAGGTTGAATGGAGACGAAGAGAATGGAGCGAAACCGCTCTCGGTGGACTCCGATGTAAAGAGTCTAGCTTCTGGGGGAGAAGTTCACTTTTATTGCAGAAGTTGCTCATATAAGTTGACTGCAAGTCCCCTTAGCACTTTTGTGGAAATGCCTTCAGTCAACTGGCGAGAGGTGGCGGATAACTGGTTTGGGAATTGCTGCTGTTCGTTTGGAGAAGTTAGTGAGAAGCTGGTTGCTGGATATGCAAATTCTTACACAAGTAAGATTGGTGTGTGCCTGGTGAGCTCTACAAATATTATGCTTTGCAAAGATGATCTTGTTGAATGTGAGTTTCTCGAATCGGGTGTATGTGAAAGACAGGATAATGAATCAGATGCCAGTGGTGAGCGTGGTCCTAATGAATCTGAACTTGCTCCTGGAAGTAATGGTGGATGTAATGAGATACTTAAGTCAGAAAATAAGGAAAAGTATATAGATGCCGATTGTGAAAGTGACGCTACTAAGGAAGAAAGTAACAGTGAGGTTGTGCCTCATAGGGGTTCAGAATCGGATTGTTCTGTGACATCAGTAGCAGTATACTGTGATGATATGGAAAGCCATGTGCAAAACTATGATGGTGAAGGCTGCAGGCATCATTTGTCTGAATCTTCTACAGAGCAAAATACGGAGATTCTGAAAAATCACAAATCTTTGCTAAATGGGTTTCTTGAAAATATTTTCATGGTTAGGTCCTCCAATCTTTCAGTAGATGTTGAATGGGTTGAATTTTTCTGCCCTCAGTGTTCATCTCTCCTTGGAGCTTACCCTTGTGATAATGGTGGCAGACTTATTGATGGTGGTGTTCGATTGTTTAAATGCAGTATTGCAACTAGTCTTCCAGTTGGTGGGACAAGAGACATTTTCAGGAAGTATACCTTGGAGAGAATGTTTGCAAACCAGCTACTCGAATGTGCAAAAGATCAGTTATCATTTAGGACCGTGGTTAGGGACCTGAAACAAAGATCTCCAATGCTGCAAATTGTTCTCATAAATACAAATTCTTGGTCTGGCACTGGTCATTGTTTGGCCACACAGGGCAATACCAAATCAGTACCAAAGATAGATCTGCATCCTGTCATCAAGGTGCTATTTTCCGAGTGCAGCAGCAGCACTGAGTCTCAATTAAGGATGCTGGAAGATTCAATAACAAAGGATGTAGCTGAGGAGGTTTTCATGCTGACACATCAGATAGAAGAATTAATCAAGTCTCTGTCAGCAAGACGGGATATACTTCCACCTTCATACTCATCCCTCGAGGGTTTATCTTTGTCATCCATGCCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT_2

Protein Analysis

490

Amino Acids

53.95

Weight (kDa)

4.7

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT_2 PF09814 66 - 479 7.5e-21 HECT-like Ubiquitin-conjugating enzyme (E2)-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26750
fragaria_vesca FvH4_1g12740
malus_domestica MD15G1254500.v1.1
prunus_persica Prupe.7G159100_v2.0.a1 Prupe.7G159100_v2.0.a1
pyrus_communis pycom15g22360
rosa_chinensis RchiOBHm_Chr2g0100811
rosa_laevigata RLG00000017013
rosa_multiflora Rmu_sc0001659.1_g000020
rosa_roxburghii Rroxscaffold_2G00141840
rosa_rugosa Rorug02G0093000
rosa_samantha Rh2AG140600 Rh2BG145600 Rh2CG146600 Rh2DG146000
rosa_wichuraiana Rw2G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1436
AccB7I CCANNNNNTGG 1 cut(s) 1028
AccBSI CCGCTC 1 cut(s) 181
AccI GTMKAC 2 cut(s) 720, 1055
AciI CCGC 2 cut(s) 179, 320
AcoI YGGCCR 1 cut(s) 1209
AcsI RAATTY 3 cut(s) 391, 908, 1180
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 1 cut(s) 1236
AfiI CCNNNNNNNGG 4 cut(s) 64, 680, 1028, 1128
AflII CTTAAG 1 cut(s) 590
AgsI TTSAA 7 cut(s) 43, 151, 467, 854, 899, 908, 1326
AhdI GACNNNNNGTC 2 cut(s) 25, 267
AhlI ACTAGT 1 cut(s) 1013
AjnI CCWGG 2 cut(s) 420, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 1043, 1075
AleI CACNNNNGTG 1 cut(s) 284
AluBI AGCT 8 cut(s) 39, 73, 212, 373, 429, 944, 1084, 1343
AluI AGCT 8 cut(s) 39, 73, 212, 373, 429, 944, 1084, 1343
Alw21I GWGCWC 2 cut(s) 111, 431
Alw26I GTCTC 4 cut(s) 150, 1032, 1305, 1395
Ama87I CYCGRG 1 cut(s) 1436
AoxI GGCC 1 cut(s) 1209
ApeKI GCWGC 6 cut(s) 342, 768, 1156, 1284, 1287, 1290
ApoI RAATTY 3 cut(s) 391, 908, 1180
ArsI GACNNNNNNTTYG 2 cut(s) 1172, 1204
AseI ATTAAT 1 cut(s) 1382
AspA2I CCTAGG 1 cut(s) 5
AspS9I GGNCC 4 cut(s) 536, 873, 1119, 1132
AsuHPI GGTGA 3 cut(s) 436, 539, 773
AvaI CYCGRG 1 cut(s) 1436
AvaII GGWCC 4 cut(s) 536, 873, 1119, 1132
AvrII CCTAGG 1 cut(s) 5
BaeI ACNNNNGTAYC 2 cut(s) 578, 611
BalI TGGCCA 1 cut(s) 1211
BanII GRGCYC 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 1010
Bbv12I GWGCWC 2 cut(s) 111, 431
BbvCI CCTCAGC 1 cut(s) 1344
BbvI GCAGC 6 cut(s) 329, 755, 1143, 1296, 1299, 1302
BccI CCATC 2 cut(s) 752, 971
BciT130I CCWGG 2 cut(s) 422, 561
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 4 cut(s) 150, 1032, 1305, 1395
BcuI ACTAGT 1 cut(s) 1013
BfaI CTAG 3 cut(s) 6, 209, 1014
BfmI CTRYAG 2 cut(s) 769, 795
BfrI CTTAAG 1 cut(s) 590
BglII AGATCT 2 cut(s) 1145, 1246
BisI GCNGC 6 cut(s) 343, 769, 1157, 1285, 1288, 1291
BlnI CCTAGG 1 cut(s) 5
BlsI GCNGC 6 cut(s) 344, 770, 1158, 1286, 1289, 1292
Bme1390I CCNGG 2 cut(s) 422, 561
Bme18I GGWCC 4 cut(s) 536, 873, 1119, 1132
BmeRI GACNNNNNGTC 2 cut(s) 25, 267
BmeT110I CYCGRG 1 cut(s) 1436
BmgT120I GGNCC 4 cut(s) 536, 873, 1119, 1132
BmiI GGNNCC 1 cut(s) 1133
BmrFI CCNGG 2 cut(s) 422, 561
BmsI GCATC 5 cut(s) 508, 610, 784, 1261, 1302
BpiI GAAGAC 1 cut(s) 1010
Bpu10I CCTNAGC 2 cut(s) 275, 1344
BsaBI GATNNNNATC 1 cut(s) 1251
BsaJI CCNNGG 5 cut(s) 5, 48, 937, 1059, 1122
BsaXI ACNNNNNCTCC 2 cut(s) 1055, 1085
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 680, 1028, 1128
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 5 cut(s) 311, 332, 522, 1022, 1201
Bse8I GATNNNNATC 1 cut(s) 1251
BseBI CCWGG 2 cut(s) 422, 561
BseDI CCNNGG 5 cut(s) 5, 48, 937, 1059, 1122
BseGI GGATG 7 cut(s) 58, 581, 1252, 1317, 1342, 1430, 1454
BseJI GATNNNNATC 1 cut(s) 1251
BseLI CCNNNNNNNGG 4 cut(s) 64, 680, 1028, 1128
BseMII CTCAG 4 cut(s) 142, 935, 1287, 1335
BseNI ACTGG 5 cut(s) 311, 332, 522, 1022, 1201
BseRI GAGGAG 1 cut(s) 1361
BseXI GCAGC 6 cut(s) 329, 755, 1143, 1296, 1299, 1302
BsgI GTGCAG 1 cut(s) 1303
BshFI GGCC 1 cut(s) 1211
BsiHKAI GWGCWC 2 cut(s) 111, 431
BsiHKCI CYCGRG 1 cut(s) 1436
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 3 cut(s) 255, 1045, 1145
BslI CCNNNNNNNGG 4 cut(s) 64, 680, 1028, 1128
BsmAI GTCTC 4 cut(s) 150, 1032, 1305, 1395
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 3 cut(s) 255, 1045, 1145
BsnI GGCC 1 cut(s) 1211
BsoBI CYCGRG 1 cut(s) 1436
Bsp1286I GDGCHC 2 cut(s) 111, 431
Bsp143I GATC 5 cut(s) 94, 457, 1102, 1145, 1246
BspACI CCGC 2 cut(s) 179, 320
BspANI GGCC 1 cut(s) 1211
BspCNI CTCAG 4 cut(s) 141, 934, 1288, 1336
BspLI GGNNCC 1 cut(s) 1133
BspMAI CTGCAG 1 cut(s) 773
BspTI CTTAAG 1 cut(s) 590
BsrBI CCGCTC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 5 cut(s) 311, 332, 522, 1022, 1201
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 5 cut(s) 5, 48, 937, 1059, 1122
BssMI GATC 5 cut(s) 94, 457, 1102, 1145, 1246
BssNAI GTATAC 2 cut(s) 721, 1056
BssT1I CCWWGG 4 cut(s) 5, 48, 937, 1059
Bst1107I GTATAC 2 cut(s) 721, 1056
Bst2UI CCWGG 2 cut(s) 422, 561
Bst4CI ACNGT 3 cut(s) 662, 725, 1123
Bst6I CTCTTC 1 cut(s) 156
BstAFI CTTAAG 1 cut(s) 590
BstAPI GCANNNNNTGC 1 cut(s) 246
BstC8I GCNNGC 2 cut(s) 75, 773
BstDEI CTNAG 6 cut(s) 128, 275, 645, 921, 1296, 1344
BstDSI CCRYGG 1 cut(s) 1122
BstF5I GGATG 7 cut(s) 58, 581, 1252, 1317, 1342, 1430, 1454
BstKTI GATC 5 cut(s) 97, 460, 1105, 1148, 1249
BstMAI GTCTC 4 cut(s) 150, 1032, 1305, 1395
BstMBI GATC 5 cut(s) 94, 457, 1102, 1145, 1246
BstMWI GCNNNNNNNGC 4 cut(s) 246, 386, 426, 1290
BstNI CCWGG 2 cut(s) 422, 561
BstSCI CCNGG 2 cut(s) 420, 559
BstSFI CTRYAG 2 cut(s) 769, 795
BstV1I GCAGC 6 cut(s) 329, 755, 1143, 1296, 1299, 1302
BstV2I GAAGAC 1 cut(s) 1010
BstX2I RGATCY 2 cut(s) 1145, 1246
BstYI RGATCY 2 cut(s) 1145, 1246
BstZ17I GTATAC 2 cut(s) 721, 1056
BsuRI GGCC 1 cut(s) 1211
BtgI CCRYGG 1 cut(s) 1122
BtsCI GGATG 7 cut(s) 58, 581, 1252, 1317, 1342, 1430, 1454
BtsIMutI CAGTG 5 cut(s) 529, 667, 929, 1194, 1293
Cac8I GCNNGC 2 cut(s) 75, 773
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 4 cut(s) 536, 873, 1119, 1132
CseI GACGC 1 cut(s) 647
Csp6I GTAC 1 cut(s) 1235
CviAII CATG 4 cut(s) 743, 865, 1357, 1459
CviQI GTAC 1 cut(s) 1235
DdeI CTNAG 6 cut(s) 128, 275, 645, 921, 1296, 1344
DpnI GATC 5 cut(s) 96, 459, 1104, 1147, 1248
DpnII GATC 5 cut(s) 94, 457, 1102, 1145, 1246
DraI TTTAAA 1 cut(s) 997
DriI GACNNNNNGTC 2 cut(s) 25, 267
EaeI YGGCCR 1 cut(s) 1209
Eam1104I CTCTTC 1 cut(s) 156
Eam1105I GACNNNNNGTC 2 cut(s) 25, 267
EarI CTCTTC 1 cut(s) 156
EciI GGCGGA 1 cut(s) 335
Ecl136II GAGCTC 1 cut(s) 429
Eco130I CCWWGG 4 cut(s) 5, 48, 937, 1059
Eco24I GRGCYC 1 cut(s) 431
Eco47I GGWCC 4 cut(s) 536, 873, 1119, 1132
Eco53kI GAGCTC 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 1436
EcoICRI GAGCTC 1 cut(s) 429
EcoO109I RGGNCCY 2 cut(s) 873, 1132
EcoRII CCWGG 2 cut(s) 420, 559
EcoT14I CCWWGG 4 cut(s) 5, 48, 937, 1059
EcoT38I GRGCYC 1 cut(s) 431
ErhI CCWWGG 4 cut(s) 5, 48, 937, 1059
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 4 cut(s) 746, 868, 1360, 1462
FalI AAGNNNNNCTT 4 cut(s) 444, 476, 1043, 1075
FaqI GGGAC 3 cut(s) 255, 1045, 1145
FatI CATG 4 cut(s) 742, 864, 1356, 1458
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 2 cut(s) 720, 1055
Fnu4HI GCNGC 6 cut(s) 343, 769, 1157, 1285, 1288, 1291
FokI GGATG 7 cut(s) 65, 588, 1239, 1324, 1349, 1417, 1441
FriOI GRGCYC 1 cut(s) 431
Fsp4HI GCNGC 6 cut(s) 343, 769, 1157, 1285, 1288, 1291
FspBI CTAG 3 cut(s) 6, 209, 1014
GluI GCNGC 6 cut(s) 343, 769, 1157, 1285, 1288, 1291
HaeIII GGCC 1 cut(s) 1211
HapII CCGG 1 cut(s) 32
HgaI GACGC 1 cut(s) 647
Hin1II CATG 4 cut(s) 746, 868, 1360, 1462
HincII GTYRAC 2 cut(s) 261, 304
HindII GTYRAC 2 cut(s) 261, 304
HpaII CCGG 1 cut(s) 32
HphI GGTGA 3 cut(s) 436, 539, 773
Hpy166II GTNNAC 6 cut(s) 190, 229, 261, 304, 721, 1056
Hpy188III TCNNGA 3 cut(s) 479, 851, 1048
Hpy8I GTNNAC 6 cut(s) 190, 229, 261, 304, 721, 1056
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 4 cut(s) 89, 306, 758, 1431
HpyCH4III ACNGT 3 cut(s) 662, 725, 1123
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 386, 426, 1290
HpyF3I CTNAG 6 cut(s) 128, 275, 645, 921, 1296, 1344
Hsp92II CATG 4 cut(s) 746, 868, 1360, 1462
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 5 cut(s) 94, 457, 1102, 1145, 1246
LmnI GCTCC 3 cut(s) 170, 562, 941
Lsp1109I GCAGC 6 cut(s) 329, 755, 1143, 1296, 1299, 1302
LweI GCATC 5 cut(s) 508, 610, 784, 1261, 1302
MaeI CTAG 3 cut(s) 6, 209, 1014
MaeIII GTNAC 3 cut(s) 635, 656, 703
MalI GATC 5 cut(s) 96, 459, 1104, 1147, 1248
MbiI CCGCTC 1 cut(s) 181
MboI GATC 5 cut(s) 94, 457, 1102, 1145, 1246
MboII GAAGA 7 cut(s) 74, 173, 662, 783, 1010, 1331, 1388
MflI RGATCY 2 cut(s) 1145, 1246
MhlI GDGCHC 2 cut(s) 111, 431
MlsI TGGCCA 1 cut(s) 1211
MluCI AATT 7 cut(s) 337, 391, 908, 1161, 1180, 1304, 1379
MluNI TGGCCA 1 cut(s) 1211
MlyI GAGTC 5 cut(s) 35, 113, 185, 214, 1307
Mox20I TGGCCA 1 cut(s) 1211
MscI TGGCCA 1 cut(s) 1211
MseI TTAA 4 cut(s) 591, 996, 1307, 1382
MslI CAYNNNNRTG 3 cut(s) 284, 1265, 1463
Msp20I TGGCCA 1 cut(s) 1211
MspCI CTTAAG 1 cut(s) 590
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 422, 561
MvaI CCWGG 2 cut(s) 422, 561
MwoI GCNNNNNNNGC 4 cut(s) 246, 386, 426, 1290
NdeII GATC 5 cut(s) 94, 457, 1102, 1145, 1246
NlaIII CATG 4 cut(s) 746, 868, 1360, 1462
NlaIV GGNNCC 1 cut(s) 1133
NmuCI GTSAC 2 cut(s) 635, 703
OliI CACNNNNGTG 1 cut(s) 284
PaeR7I CTCGAG 1 cut(s) 1436
PfeI GAWTC 7 cut(s) 482, 512, 545, 689, 788, 814, 1322
PflMI CCANNNNNTGG 1 cut(s) 1028
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 6 cut(s) 344, 770, 1158, 1286, 1289, 1292
PleI GAGTC 5 cut(s) 34, 113, 185, 213, 1306
PpsI GAGTC 5 cut(s) 34, 113, 185, 213, 1306
PpuMI RGGWCCY 2 cut(s) 873, 1132
PshBI ATTAAT 1 cut(s) 1382
Psp124BI GAGCTC 1 cut(s) 431
Psp5II RGGWCCY 2 cut(s) 873, 1132
Psp6I CCWGG 2 cut(s) 420, 559
PspGI CCWGG 2 cut(s) 420, 559
PspN4I GGNNCC 1 cut(s) 1133
PspPI GGNCC 4 cut(s) 536, 873, 1119, 1132
PspPPI RGGWCCY 2 cut(s) 873, 1132
PspXI VCTCGAGB 1 cut(s) 1436
PstI CTGCAG 1 cut(s) 773
PsuI RGATCY 2 cut(s) 1145, 1246
RsaI GTAC 1 cut(s) 1236
RsaNI GTAC 1 cut(s) 1235
RseI CAYNNNNRTG 3 cut(s) 284, 1265, 1463
SacI GAGCTC 1 cut(s) 431
SaqAI TTAA 4 cut(s) 591, 996, 1307, 1382
SatI GCNGC 6 cut(s) 343, 769, 1157, 1285, 1288, 1291
Sau3AI GATC 5 cut(s) 94, 457, 1102, 1145, 1246
Sau96I GGNCC 4 cut(s) 536, 873, 1119, 1132
SchI GAGTC 5 cut(s) 35, 113, 185, 214, 1307
ScrFI CCNGG 2 cut(s) 422, 561
SduI GDGCHC 2 cut(s) 111, 431
SfaNI GCATC 5 cut(s) 508, 610, 784, 1261, 1302
SfcI CTRYAG 2 cut(s) 769, 795
Sfr274I CTCGAG 1 cut(s) 1436
SinI GGWCC 4 cut(s) 536, 873, 1119, 1132
SlaI CTCGAG 1 cut(s) 1436
SmiMI CAYNNNNRTG 3 cut(s) 284, 1265, 1463
SmlI CTYRAG 2 cut(s) 590, 1436
SmoI CTYRAG 2 cut(s) 590, 1436
SpeI ACTAGT 1 cut(s) 1013
Sse9I AATT 7 cut(s) 337, 391, 908, 1161, 1180, 1304, 1379
SsiI CCGC 2 cut(s) 179, 320
SspI AATATT 2 cut(s) 439, 859
SspMI CTAG 3 cut(s) 6, 209, 1014
SstI GAGCTC 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 420, 559
StyI CCWWGG 4 cut(s) 5, 48, 937, 1059
TaaI ACNGT 3 cut(s) 662, 725, 1123
TaqI TCGA 5 cut(s) 117, 480, 988, 1089, 1437
TasI AATT 7 cut(s) 337, 391, 908, 1161, 1180, 1304, 1379
TfiI GAWTC 7 cut(s) 482, 512, 545, 689, 788, 814, 1322
Tru1I TTAA 4 cut(s) 591, 996, 1307, 1382
Tru9I TTAA 4 cut(s) 591, 996, 1307, 1382
TscAI CASTG 5 cut(s) 529, 667, 929, 1201, 1300
TseFI GTSAC 2 cut(s) 635, 703
TseI GCWGC 6 cut(s) 342, 768, 1156, 1284, 1287, 1290
Tsp45I GTSAC 2 cut(s) 635, 703
TspDTI ATGAA 6 cut(s) 525, 558, 853, 918, 1345, 1413
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 5 cut(s) 529, 667, 929, 1201, 1300
Van91I CCANNNNNTGG 1 cut(s) 1028
Vha464I CTTAAG 1 cut(s) 590
VpaK11BI GGWCC 4 cut(s) 536, 873, 1119, 1132
VspI ATTAAT 1 cut(s) 1382
XapI RAATTY 3 cut(s) 391, 908, 1180
XhoI CTCGAG 1 cut(s) 1436
XmaJI CCTAGG 1 cut(s) 5
XmiI GTMKAC 2 cut(s) 720, 1055
XspI CTAG 3 cut(s) 6, 209, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.