MD15G1254500.v1.1

HECT-like Ubiquitin-conjugating enzyme (E2)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015450
Physical Location & Seq
Forward (+)
21504021 .. 21507348
3328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1254500.v1.1.491

Sequence Viewer

Length: 1674 bp
ATGTCCATGGAGCTTCAAACCGCCGGAAATCCGAGAAAATGGCGGTTCACATGGGAAGCCCAATCTCACATCCCAATCCTCCGCCTATTCCTATTCGATTCCTGCACAAAACCCTCGACTCAATGCCGGAAGCTCACCGTCCACATAACCCCCGCAGAGTCTCTGGTCCTGGTGAGCTGGGCCGAGGACGCTCAGGAGGTCTCGCTTAGGGTTCCAATGCCTAGGGTTTTGGTGGACGCTGAGTCGCCGGTGAGCTTCAGTGCCCTAGACGATCACATTGAAGTCAAGCTCGTCCTCCTCCTCCCTGTTGATCACCCCATTGTTTTGAGCTTCGATTCTTTACTCAGTTTGGATGGAGGGGAGGAGAAAGCGTTGGAGGGCGAATTGAAGCCGCTTTCGTTGGCTTCTGAGGTCAAGAGTCTATCATCGTCTGGAGTTCACTTTTACTGCAGAAATTGCTCGTTTAAGTTGACTGCAAGTCCCCTGAGCCAGTTTGTGGAAATGCCTTCAGTCAATTGGCGAGAGGTGGCTGATAACTGGTTTGGGGCTTGCTGTTGTTCATTTGGAGGCATTAGTGAGAAGCTGGTTGTTAGATATGCAAATTCTTACATGTGTGCAAAGGGTGTCTGCCTGGTGAACTCTACAAATATTACACTATGCAAGGATGATCTTGTTGGATTTGAATTTCCTGACTTGGGTGAATGCCAAAGATACGATAGTGAATCAGATGGAAGTGGTGACAATGGTTTTACCGAGTCAGAACTTAATCTTGGAAGTAATCTCACATGCAATGAAGATTTTGCTGCAGAATCTAAATCTGAAGTTGCTAACGACGAAAGTAACAGTGAGGATGCACCTCATCTCTGCTCAGGATCAGTTTGCTCTATAAAAAATTCGTCTACACCAGGATGCTGTAATCATATGGGATGTCATGTTCAAAATTATGATGGTGACAGCTGCAGACTTTGTTTTTCTGAAATTTCTTTAGAGGACCAAAAACCTACTAAAAGTACGGAGATCCTGAAAAACCACGAATCTTTCCTAAATGGGTATCTTGAAAACATTTTTATGGTTAGGTCCTCCAATCTATCTATAGATGTTGAGTGGGTTGAATTTTTCTGCCCTCAATGTTCATCACTTCTCGGAGCTTACCCTTGTGATAATGGCAGTGCACTTGTAGATGGTGGAGTTCGATTGTTTAAATGTAATGTTTCCACTGGCCTGCCAGTTGGTGAGCCAGCGGACATATTCAGGAAGTATACACTGGAAAGTATGTTCGCAAATCAACTACTTGAGTGTGCAAAAGACGAATTATCTTTTAGGACCGTGGTTAGTGACCTAAAAAGCAGATCTCCCAGGGTGCAAATTGTTCTCATGAATACAAATTCTTGGTCTGGCACCGGTCATTGTTTGGCCAGAGAGGGCAATAAAGAACCAGTACCAAAGATAGATCTGCATCCTGTCATCAAGGTGCTATTTTCACAGTGCAGCAGCAGTACGGAATCTCAAGCAAGGATGCTGGAAGATTCAGTAACGAAGGACGTAGTTGATGAAGTATTTATGCTAACACATCAAATAGAAGAACTAATCGAGACTCTTTCTGCAAGAAAGGATATGTTTCCACCTTCCTGCTCTTCCTTACAGGGTTTATCTTTGTCATCCATGCCGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT_2

Protein Analysis

558

Amino Acids

61.39

Weight (kDa)

4.79

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT_2 PF09814 138 - 549 6e-20 HECT-like Ubiquitin-conjugating enzyme (E2)-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26750
fragaria_vesca FvH4_1g12740
malus_domestica MD15G1254500.v1.1
prunus_persica Prupe.7G159100_v2.0.a1 Prupe.7G159100_v2.0.a1
pyrus_communis pycom15g22360
rosa_chinensis RchiOBHm_Chr2g0100811
rosa_laevigata RLG00000017013
rosa_multiflora Rmu_sc0001659.1_g000020
rosa_roxburghii Rroxscaffold_2G00141840
rosa_rugosa Rorug02G0093000
rosa_samantha Rh2AG140600 Rh2BG145600 Rh2CG146600 Rh2DG146000
rosa_wichuraiana Rw2G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1397
AccB7I CCANNNNNTGG 1 cut(s) 496
AccI GTMKAC 2 cut(s) 899, 1259
AciI CCGC 6 cut(s) 21, 43, 82, 153, 392, 1241
AclWI GGATC 2 cut(s) 880, 1012
AcoI YGGCCR 1 cut(s) 1413
AcsI RAATTY 6 cut(s) 601, 683, 892, 978, 1112, 1384
AcuI CTGAAG 3 cut(s) 241, 492, 840
AfaI GTAC 3 cut(s) 1012, 1440, 1498
AfiI CCNNNNNNNGG 3 cut(s) 496, 695, 1668
AflIII ACRYGT 1 cut(s) 609
AgeI ACCGGT 1 cut(s) 1400
AgsI TTSAA 7 cut(s) 17, 281, 388, 683, 938, 1058, 1112
AhdI GACNNNNNGTC 2 cut(s) 241, 477
AjnI CCWGG 4 cut(s) 168, 630, 904, 1355
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AloI GAACNNNNNNTCC 4 cut(s) 918, 950, 1259, 1291
AluBI AGCT 9 cut(s) 13, 133, 177, 255, 289, 330, 583, 957, 1148
AluI AGCT 9 cut(s) 13, 133, 177, 255, 289, 330, 583, 957, 1148
Alw21I GWGCWC 1 cut(s) 1174
Alw26I GTCTC 3 cut(s) 165, 205, 1586
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 2 cut(s) 880, 1012
AoxI GGCC 3 cut(s) 180, 1219, 1413
ApaLI GTGCAC 1 cut(s) 1170
ApeKI GCWGC 4 cut(s) 803, 957, 1488, 1491
ApoI RAATTY 6 cut(s) 601, 683, 892, 978, 1112, 1384
ArsI GACNNNNNNTTYG 2 cut(s) 1376, 1408
AsiGI ACCGGT 1 cut(s) 1400
AspA2I CCTAGG 1 cut(s) 221
AspS9I GGNCC 5 cut(s) 166, 180, 991, 1077, 1323
AsuHPI GGTGA 9 cut(s) 127, 184, 262, 305, 646, 710, 749, 962, 1244
AvaII GGWCC 4 cut(s) 166, 991, 1077, 1323
AvrII CCTAGG 1 cut(s) 221
BaeGI GKGCMC 2 cut(s) 265, 1174
BaeI ACNNNNGTAYC 2 cut(s) 703, 736
BalI TGGCCA 1 cut(s) 1415
BanI GGYRCC 1 cut(s) 1397
Bbv12I GWGCWC 1 cut(s) 1174
BbvI GCAGC 4 cut(s) 790, 944, 1500, 1503
BccI CCATC 4 cut(s) 347, 722, 941, 1175
BciT130I CCWGG 4 cut(s) 170, 632, 906, 1357
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 3 cut(s) 165, 205, 1586
BfaI CTAG 2 cut(s) 222, 266
BfmI CTRYAG 4 cut(s) 448, 804, 958, 1092
BglII AGATCT 2 cut(s) 1349, 1450
BisI GCNGC 5 cut(s) 392, 804, 958, 1489, 1492
BlnI CCTAGG 1 cut(s) 221
BlsI GCNGC 5 cut(s) 393, 805, 959, 1490, 1493
Bme1390I CCNGG 4 cut(s) 170, 632, 906, 1357
Bme18I GGWCC 4 cut(s) 166, 991, 1077, 1323
BmeRI GACNNNNNGTC 2 cut(s) 241, 477
BmgT120I GGNCC 5 cut(s) 166, 180, 991, 1077, 1323
BmiI GGNNCC 2 cut(s) 213, 1399
BmrFI CCNGG 4 cut(s) 170, 632, 906, 1357
BmsI GCATC 4 cut(s) 841, 899, 1465, 1506
BpmI CTGGAG 1 cut(s) 453
Bpu10I CCTNAGC 4 cut(s) 192, 206, 485, 868
BpuEI CTTGAG 2 cut(s) 1313, 1491
BsaBI GATNNNNATC 1 cut(s) 1455
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 7 cut(s) 6, 183, 221, 1326, 1355, 1356, 1667
BsaWI WCCGGW 1 cut(s) 1400
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 3 cut(s) 496, 695, 1668
Bse118I RCCGGY 2 cut(s) 247, 1400
Bse1I ACTGG 6 cut(s) 490, 542, 1222, 1226, 1269, 1436
Bse3DI GCAATG 1 cut(s) 796
Bse8I GATNNNNATC 1 cut(s) 1455
BseBI CCWGG 4 cut(s) 170, 632, 906, 1357
BseDI CCNNGG 7 cut(s) 6, 183, 221, 1326, 1355, 1356, 1667
BseGI GGATG 9 cut(s) 69, 358, 670, 856, 914, 932, 1456, 1521, 1658
BseJI GATNNNNATC 1 cut(s) 1455
BseLI CCNNNNNNNGG 3 cut(s) 496, 695, 1668
BseMI GCAATG 1 cut(s) 796
BseMII CTCAG 6 cut(s) 206, 231, 358, 399, 476, 882
BseNI ACTGG 6 cut(s) 490, 542, 1222, 1226, 1269, 1436
BseRI GAGGAG 3 cut(s) 287, 290, 377
BseSI GKGCMC 2 cut(s) 265, 1174
BseXI GCAGC 4 cut(s) 790, 944, 1500, 1503
BseYI CCCAGC 1 cut(s) 177
BsgI GTGCAG 2 cut(s) 88, 1507
BshFI GGCC 3 cut(s) 182, 1221, 1415
BshNI GGYRCC 1 cut(s) 1397
BshTI ACCGGT 1 cut(s) 1400
BsiHKAI GWGCWC 1 cut(s) 1174
BsiSI CCGG 4 cut(s) 24, 127, 248, 1401
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 3 cut(s) 496, 695, 1668
BsmAI GTCTC 3 cut(s) 165, 205, 1586
BsmFI GGGAC 1 cut(s) 465
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 3 cut(s) 182, 1221, 1415
Bso31I GGTCTC 1 cut(s) 205
Bsp1286I GDGCHC 2 cut(s) 265, 1174
Bsp143I GATC 7 cut(s) 271, 310, 667, 872, 1017, 1349, 1450
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 6 cut(s) 21, 43, 82, 153, 392, 1241
BspANI GGCC 3 cut(s) 182, 1221, 1415
BspCNI CTCAG 6 cut(s) 205, 232, 357, 400, 477, 881
BspHI TCATGA 1 cut(s) 1374
BspLI GGNNCC 2 cut(s) 213, 1399
BspMAI CTGCAG 3 cut(s) 452, 808, 962
BspPI GGATC 2 cut(s) 880, 1012
BspQI GCTCTTC 1 cut(s) 1639
BspT107I GGYRCC 1 cut(s) 1397
BspTNI GGTCTC 1 cut(s) 205
BsrDI GCAATG 1 cut(s) 796
BsrFI RCCGGY 2 cut(s) 247, 1400
BsrI ACTGG 6 cut(s) 490, 542, 1222, 1226, 1269, 1436
BssAI RCCGGY 2 cut(s) 247, 1400
BssECI CCNNGG 7 cut(s) 6, 183, 221, 1326, 1355, 1356, 1667
BssMI GATC 7 cut(s) 271, 310, 667, 872, 1017, 1349, 1450
BssNAI GTATAC 1 cut(s) 1260
BssT1I CCWWGG 2 cut(s) 6, 221
Bst1107I GTATAC 1 cut(s) 1260
Bst2UI CCWGG 4 cut(s) 170, 632, 906, 1357
Bst4CI ACNGT 4 cut(s) 139, 845, 1327, 1485
Bst6I CTCTTC 1 cut(s) 1639
BstAPI GCANNNNNTGC 1 cut(s) 456
BstC8I GCNNGC 3 cut(s) 550, 1223, 1239
BstDEI CTNAG 7 cut(s) 192, 206, 240, 344, 408, 485, 868
BstDSI CCRYGG 2 cut(s) 6, 1326
BstF5I GGATG 9 cut(s) 69, 358, 670, 856, 914, 932, 1456, 1521, 1658
BstKTI GATC 7 cut(s) 274, 313, 670, 875, 1020, 1352, 1453
BstMAI GTCTC 3 cut(s) 165, 205, 1586
BstMBI GATC 7 cut(s) 271, 310, 667, 872, 1017, 1349, 1450
BstMWI GCNNNNNNNGC 2 cut(s) 188, 456
BstNI CCWGG 4 cut(s) 170, 632, 906, 1357
BstNSI RCATGY 2 cut(s) 613, 789
BstSCI CCNGG 4 cut(s) 168, 630, 904, 1355
BstSFI CTRYAG 4 cut(s) 448, 804, 958, 1092
BstSLI GKGCMC 2 cut(s) 265, 1174
BstV1I GCAGC 4 cut(s) 790, 944, 1500, 1503
BstX2I RGATCY 3 cut(s) 1017, 1349, 1450
BstYI RGATCY 3 cut(s) 1017, 1349, 1450
BstZ17I GTATAC 1 cut(s) 1260
BsuRI GGCC 3 cut(s) 182, 1221, 1415
BtgI CCRYGG 2 cut(s) 6, 1326
BtsCI GGATG 9 cut(s) 69, 358, 670, 856, 914, 932, 1456, 1521, 1658
BtsI GCAGTG 1 cut(s) 1174
BtsIMutI CAGTG 6 cut(s) 265, 850, 1174, 1215, 1262, 1490
Cac8I GCNNGC 3 cut(s) 550, 1223, 1239
CciI TCATGA 1 cut(s) 1374
Cfr10I RCCGGY 2 cut(s) 247, 1400
Cfr13I GGNCC 5 cut(s) 166, 180, 991, 1077, 1323
CseI GACGC 2 cut(s) 197, 245
Csp6I GTAC 3 cut(s) 1011, 1439, 1497
CspAI ACCGGT 1 cut(s) 1400
CviAII CATG 7 cut(s) 7, 51, 610, 786, 932, 1375, 1663
CviQI GTAC 3 cut(s) 1011, 1439, 1497
DdeI CTNAG 7 cut(s) 192, 206, 240, 344, 408, 485, 868
DpnI GATC 7 cut(s) 273, 312, 669, 874, 1019, 1351, 1452
DpnII GATC 7 cut(s) 271, 310, 667, 872, 1017, 1349, 1450
DraI TTTAAA 1 cut(s) 1201
DriI GACNNNNNGTC 2 cut(s) 241, 477
EaeI YGGCCR 1 cut(s) 1413
Eam1104I CTCTTC 1 cut(s) 1639
Eam1105I GACNNNNNGTC 2 cut(s) 241, 477
EarI CTCTTC 1 cut(s) 1639
EciI GGCGGA 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 6, 221
Eco31I GGTCTC 1 cut(s) 205
Eco47I GGWCC 4 cut(s) 166, 991, 1077, 1323
Eco57I CTGAAG 3 cut(s) 241, 492, 840
EcoO109I RGGNCCY 1 cut(s) 1077
EcoRII CCWGG 4 cut(s) 168, 630, 904, 1355
EcoT14I CCWWGG 2 cut(s) 6, 221
ErhI CCWWGG 2 cut(s) 6, 221
FaeI CATG 7 cut(s) 10, 54, 613, 789, 935, 1378, 1666
FaqI GGGAC 1 cut(s) 465
FatI CATG 7 cut(s) 6, 50, 609, 785, 931, 1374, 1662
FauI CCCGC 1 cut(s) 160
FauNDI CATATG 1 cut(s) 921
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 2 cut(s) 899, 1259
Fnu4HI GCNGC 5 cut(s) 392, 804, 958, 1489, 1492
FokI GGATG 9 cut(s) 56, 365, 677, 863, 921, 939, 1443, 1528, 1645
Fsp4HI GCNGC 5 cut(s) 392, 804, 958, 1489, 1492
FspBI CTAG 2 cut(s) 222, 266
GluI GCNGC 5 cut(s) 392, 804, 958, 1489, 1492
GsaI CCCAGC 1 cut(s) 181
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 3 cut(s) 182, 1221, 1415
HapII CCGG 4 cut(s) 24, 127, 248, 1401
HgaI GACGC 2 cut(s) 197, 245
Hin1II CATG 7 cut(s) 10, 54, 613, 789, 935, 1378, 1666
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HpaII CCGG 4 cut(s) 24, 127, 248, 1401
HphI GGTGA 9 cut(s) 127, 184, 262, 305, 646, 710, 749, 962, 1244
Hpy166II GTNNAC 9 cut(s) 48, 142, 235, 439, 471, 637, 900, 1172, 1260
Hpy188I TCNGA 7 cut(s) 33, 409, 727, 760, 820, 976, 1145
Hpy8I GTNNAC 9 cut(s) 48, 142, 235, 439, 471, 637, 900, 1172, 1260
Hpy99I CGWCG 1 cut(s) 836
HpyAV CCTTC 3 cut(s) 516, 1531, 1635
HpyCH4III ACNGT 4 cut(s) 139, 845, 1327, 1485
HpyCH4IV ACGT 1 cut(s) 1542
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 456
HpyF3I CTNAG 7 cut(s) 192, 206, 240, 344, 408, 485, 868
HpySE526I ACGT 1 cut(s) 1542
Hsp92II CATG 7 cut(s) 10, 54, 613, 789, 935, 1378, 1666
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 7 cut(s) 271, 310, 667, 872, 1017, 1349, 1450
LguI GCTCTTC 1 cut(s) 1639
LmnI GCTCC 2 cut(s) 10, 1145
Lsp1109I GCAGC 4 cut(s) 790, 944, 1500, 1503
LweI GCATC 4 cut(s) 841, 899, 1465, 1506
MaeI CTAG 2 cut(s) 222, 266
MaeII ACGT 1 cut(s) 1542
MaeIII GTNAC 5 cut(s) 737, 839, 950, 1334, 1531
MalI GATC 7 cut(s) 273, 312, 669, 874, 1019, 1351, 1452
MboI GATC 7 cut(s) 271, 310, 667, 872, 1017, 1349, 1450
MboII GAAGA 4 cut(s) 806, 1535, 1592, 1626
MfeI CAATTG 1 cut(s) 514
MflI RGATCY 3 cut(s) 1017, 1349, 1450
MhlI GDGCHC 2 cut(s) 265, 1174
MlsI TGGCCA 1 cut(s) 1415
MluNI TGGCCA 1 cut(s) 1415
MlyI GAGTC 6 cut(s) 112, 167, 251, 427, 764, 1588
MmeI TCCRAC 2 cut(s) 354, 655
Mox20I TGGCCA 1 cut(s) 1415
MscI TGGCCA 1 cut(s) 1415
MseI TTAA 3 cut(s) 465, 765, 1200
MslI CAYNNNNRTG 3 cut(s) 907, 1067, 1469
Msp20I TGGCCA 1 cut(s) 1415
MspA1I CMGCKG 2 cut(s) 957, 1241
MspI CCGG 4 cut(s) 24, 127, 248, 1401
MspR9I CCNGG 4 cut(s) 170, 632, 906, 1357
MunI CAATTG 1 cut(s) 514
Mva1269I GAATGC 1 cut(s) 707
MvaI CCWGG 4 cut(s) 170, 632, 906, 1357
MwoI GCNNNNNNNGC 2 cut(s) 188, 456
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 921
NdeII GATC 7 cut(s) 271, 310, 667, 872, 1017, 1349, 1450
NlaIII CATG 7 cut(s) 10, 54, 613, 789, 935, 1378, 1666
NlaIV GGNNCC 2 cut(s) 213, 1399
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 3 cut(s) 737, 950, 1334
NspI RCATGY 2 cut(s) 613, 789
PagI TCATGA 1 cut(s) 1374
PasI CCCWGGG 1 cut(s) 1356
PciI ACATGT 1 cut(s) 609
PciSI GCTCTTC 1 cut(s) 1639
PctI GAATGC 1 cut(s) 707
PfeI GAWTC 7 cut(s) 98, 335, 722, 809, 1034, 1502, 1526
PflMI CCANNNNNTGG 1 cut(s) 496
PinAI ACCGGT 1 cut(s) 1400
PkrI GCNGC 5 cut(s) 393, 805, 959, 1490, 1493
PleI GAGTC 6 cut(s) 112, 166, 250, 426, 763, 1588
PpsI GAGTC 6 cut(s) 112, 166, 250, 426, 763, 1588
PpuMI RGGWCCY 1 cut(s) 1077
PscI ACATGT 1 cut(s) 609
Psp5II RGGWCCY 1 cut(s) 1077
Psp6I CCWGG 4 cut(s) 168, 630, 904, 1355
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 4 cut(s) 168, 630, 904, 1355
PspN4I GGNNCC 2 cut(s) 213, 1399
PspPI GGNCC 5 cut(s) 166, 180, 991, 1077, 1323
PspPPI RGGWCCY 1 cut(s) 1077
PstI CTGCAG 3 cut(s) 452, 808, 962
PsuI RGATCY 3 cut(s) 1017, 1349, 1450
PvuII CAGCTG 1 cut(s) 957
RsaI GTAC 3 cut(s) 1012, 1440, 1498
RsaNI GTAC 3 cut(s) 1011, 1439, 1497
RseI CAYNNNNRTG 3 cut(s) 907, 1067, 1469
SapI GCTCTTC 1 cut(s) 1639
SaqAI TTAA 3 cut(s) 465, 765, 1200
SatI GCNGC 5 cut(s) 392, 804, 958, 1489, 1492
Sau3AI GATC 7 cut(s) 271, 310, 667, 872, 1017, 1349, 1450
Sau96I GGNCC 5 cut(s) 166, 180, 991, 1077, 1323
SchI GAGTC 6 cut(s) 112, 167, 251, 427, 764, 1588
ScrFI CCNGG 4 cut(s) 170, 632, 906, 1357
SduI GDGCHC 2 cut(s) 265, 1174
SfaNI GCATC 4 cut(s) 841, 899, 1465, 1506
SfcI CTRYAG 4 cut(s) 448, 804, 958, 1092
SgrAI CRCCGGYG 1 cut(s) 247
SinI GGWCC 4 cut(s) 166, 991, 1077, 1323
SmiMI CAYNNNNRTG 3 cut(s) 907, 1067, 1469
SmlI CTYRAG 2 cut(s) 1292, 1506
SmoI CTYRAG 2 cut(s) 1292, 1506
SsiI CCGC 6 cut(s) 21, 43, 82, 153, 392, 1241
SspI AATATT 1 cut(s) 649
SspMI CTAG 2 cut(s) 222, 266
StyD4I CCNGG 4 cut(s) 168, 630, 904, 1355
StyI CCWWGG 2 cut(s) 6, 221
TaaI ACNGT 4 cut(s) 139, 845, 1327, 1485
TaiI ACGT 1 cut(s) 1545
TaqI TCGA 5 cut(s) 96, 116, 333, 1192, 1590
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 7 cut(s) 98, 335, 722, 809, 1034, 1502, 1526
Tru1I TTAA 3 cut(s) 465, 765, 1200
Tru9I TTAA 3 cut(s) 465, 765, 1200
TscAI CASTG 6 cut(s) 265, 850, 1174, 1222, 1269, 1490
TseFI GTSAC 3 cut(s) 737, 950, 1334
TseI GCWGC 4 cut(s) 803, 957, 1488, 1491
Tsp45I GTSAC 3 cut(s) 737, 950, 1334
TspDTI ATGAA 5 cut(s) 549, 807, 1122, 1391, 1566
TspGWI ACGGA 2 cut(s) 1028, 1514
TspRI CASTG 6 cut(s) 265, 850, 1174, 1222, 1269, 1490
Van91I CCANNNNNTGG 1 cut(s) 496
VneI GTGCAC 1 cut(s) 1170
VpaK11BI GGWCC 4 cut(s) 166, 991, 1077, 1323
XapI RAATTY 6 cut(s) 601, 683, 892, 978, 1112, 1384
XceI RCATGY 2 cut(s) 613, 789
XmaJI CCTAGG 1 cut(s) 221
XmiI GTMKAC 2 cut(s) 899, 1259
XspI CTAG 2 cut(s) 222, 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.