FvH4_1g12740

HECT-like Ubiquitin-conjugating enzyme (E2)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015450
Physical Location & Seq
Forward (+)
7015176 .. 7018170
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12740.t1

Sequence Viewer

Length: 1716 bp
ATGACCCAGAAGCTTGAAACCGCCGAAAGCAGCACAGAATGGAGGTTCACATGGGAATCCCAATCCCACATCCCAACCCTCCGCCTCTTCCTCTTCAACTCCTCCACCAACTCCTCCACCAACCCCTCCACCCAATGCCACAACCTCACCGTCCACCTCAGCCTCCCTCACTCTCTCCTCCTCCTCACCTGGACCGCCGCCGCCGACGCCGCCGTGGTCTCTCTCTGTGTTCCCATGCCTAGGGTTTTGCTCGACGACGAGTCTCCGGTCAGCTTCAAAGCCTTGGACGACCACATTGAGGTCAAGCTCGCCCTTCTTCTACCTGTTGATCACTCTATTGTGCTCAACTTTGAGTCTCTACTCAGTTTGGGTGAGATGAGGTTGGATGAAGATGAAGACAATGGTTTGAAACCGCTCTCGGTGGACTCCGATGTGAAGAGTTTATCTGCTAGGGGAGAGGTTCACTTTTATTGCAGAAGTTGCTCATATAAGCTGACTGCAAGTCCTCTTAGCACTTTTGTGGAAATGCCATCAGTCAACTGGCGGGAGGTGGCAGACAACTGGTTTGGGAACTGCTGCTGTTCGTTTGGAGAAGTTAGTGAGAAGCTGGTTGCTGGATATGCGAATTCTTACACAAGTAAGATGGGTGTGTGCCTGGTGAGCTCTACTAATATCACACTTTGCAAAGATGATCTTGTTGGATGTGAATTTCCGGACTCAGGTGTATGTGAAAGACGTGATAATGAATCAGATGCCAGTGGTGAGTGTGGTCCAAAAGAGTCTGAACTTGCTCCTGGAAGTAATGTTAGATGCAATGAGATACCAAAGTCGGAAAATAAGGAAAAATATGTGGATGCCAGTTGTAAAAGTGATGCTACTGAGGATGAAGATAAGAGTGAGGGCGTACCTCATAGGTGTTCGGAATCAGATTGTTCTGTTAGCGAATCCAGGAAAGAACGGGATGGAGATTGTGATGATATGGAAAGTCATGCTCAAAACTATGATGGTGAATGCTGCAGTCATCATTTGTCTAAATCCTCTTCAGAGCAAAACACAGAGATTCTGAAAAATCACAAATCTTTGCTAAATGGGTATCTTGAAAATATTTTCATGGTCAGGTCCTCCAATCTTTCAGTAGATGTTGAATGGGTTGAATTTTTCTGCCCTCATTGTTCATCTCTCCTTGGAGCTTACCCTTGTGATAATGGTGGCAGACTTATTGATGGTGGTGTTCGATTGTTTAAATGCAATATTGCCACTAGTCTTCCAGTTGGAGGGACAAGAGATGTTTTCAGGAAGTATACCTTGGAGAGAATGTTTGCAAACCAGCTACTTGAATGTGCAAAAGATCAATTATCATACAGGACCGTGGTTAAGGACCTGAAACTCAGATCTCCAATGCTACAAATTGTTCTCATTAATACAAATTCTTGGTCTGGCACTGGTCATTGTTTGGCCACACAGGGCAATACTGAACCAGTACCAAAGATTGATCTGCATCCTGTCATCAAGGTGCTATTTTCCGAGTGCAGCAGCAGCACTGAAGCTCAATTAAGGATGTTGGAAGATTCAGTAACAAAGGATGTAGTTGAGGAGGTTTTCATGCTGACACATCAGATAGAAGAATTAATGAAGTTTCTGTCAGCAAGACGGGATACACTTCCACCTTCATGCTCATCCCTTGAGGGTTTATCTTTGTCATCCATTCCGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT_2

Protein Analysis

572

Amino Acids

63.24

Weight (kDa)

4.87

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26750
fragaria_vesca FvH4_1g12740
malus_domestica MD15G1254500.v1.1
prunus_persica Prupe.7G159100_v2.0.a1 Prupe.7G159100_v2.0.a1
pyrus_communis pycom15g22360
rosa_chinensis RchiOBHm_Chr2g0100811
rosa_laevigata RLG00000017013
rosa_multiflora Rmu_sc0001659.1_g000020
rosa_roxburghii Rroxscaffold_2G00141840
rosa_rugosa Rorug02G0093000
rosa_samantha Rh2AG140600 Rh2BG145600 Rh2CG146600 Rh2DG146000
rosa_wichuraiana Rw2G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 415
AccI GTMKAC 1 cut(s) 1301
AccIII TCCGGA 1 cut(s) 712
AciI CCGC 8 cut(s) 21, 82, 195, 198, 201, 210, 413, 544
AcoI YGGCCR 1 cut(s) 1455
AcsI RAATTY 4 cut(s) 625, 707, 1154, 1426
AcuI CTGAAG 2 cut(s) 1026, 1563
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 906, 1482
AfiI CCNNNNNNNGG 4 cut(s) 240, 298, 719, 1274
AgsI TTSAA 8 cut(s) 17, 97, 277, 409, 1100, 1145, 1154, 1337
AhdI GACNNNNNGTC 2 cut(s) 259, 501
AhlI ACTAGT 1 cut(s) 1259
AjiI CACGTC 1 cut(s) 737
AjnI CCWGG 4 cut(s) 188, 654, 793, 947
AjuI GAANNNNNNNTTGG 2 cut(s) 1289, 1321
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 9 cut(s) 13, 273, 307, 493, 607, 663, 1190, 1330, 1547
AluI AGCT 9 cut(s) 13, 273, 307, 493, 607, 663, 1190, 1330, 1547
Alw21I GWGCWC 2 cut(s) 345, 665
Alw26I GTCTC 3 cut(s) 223, 267, 360
Aor13HI TCCGGA 1 cut(s) 712
AoxI GGCC 1 cut(s) 1455
ApeKI GCWGC 6 cut(s) 30, 576, 1014, 1530, 1533, 1536
ApoI RAATTY 4 cut(s) 625, 707, 1154, 1426
ArsI GACNNNNNNTTYG 4 cut(s) 548, 580, 1418, 1450
AseI ATTAAT 2 cut(s) 1419, 1628
AspA2I CCTAGG 1 cut(s) 239
AspS9I GGNCC 5 cut(s) 192, 770, 1119, 1365, 1378
AsuHPI GGTGA 6 cut(s) 139, 178, 383, 670, 773, 1019
AvaII GGWCC 5 cut(s) 192, 770, 1119, 1365, 1378
AvrII CCTAGG 1 cut(s) 239
BaeI ACNNNNGTAYC 2 cut(s) 812, 845
BalI TGGCCA 1 cut(s) 1457
BanII GRGCYC 1 cut(s) 665
BbsI GAAGAC 2 cut(s) 402, 1256
Bbv12I GWGCWC 2 cut(s) 345, 665
BbvCI CCTCAGC 1 cut(s) 158
BbvI GCAGC 6 cut(s) 42, 563, 1001, 1542, 1545, 1548
BccI CCATC 5 cut(s) 538, 637, 956, 998, 1217
BceAI ACGGC 1 cut(s) 197
BciT130I CCWGG 4 cut(s) 190, 656, 795, 949
BciVI GTATCC 1 cut(s) 1648
BclI TGATCA 1 cut(s) 328
BcoDI GTCTC 3 cut(s) 223, 267, 360
BcuI ACTAGT 1 cut(s) 1259
BfaI CTAG 3 cut(s) 240, 450, 1260
BfmI CTRYAG 1 cut(s) 1015
BfuI GTATCC 1 cut(s) 1648
BglII AGATCT 1 cut(s) 1391
BisI GCNGC 9 cut(s) 31, 198, 201, 210, 577, 1015, 1531, 1534, 1537
BlnI CCTAGG 1 cut(s) 239
BlsI GCNGC 9 cut(s) 32, 199, 202, 211, 578, 1016, 1532, 1535, 1538
Bme1390I CCNGG 4 cut(s) 190, 656, 795, 949
Bme18I GGWCC 5 cut(s) 192, 770, 1119, 1365, 1378
BmeRI GACNNNNNGTC 2 cut(s) 259, 501
BmgBI CACGTC 1 cut(s) 737
BmgT120I GGNCC 5 cut(s) 192, 770, 1119, 1365, 1378
BmrFI CCNGG 4 cut(s) 190, 656, 795, 949
BmsI GCATC 5 cut(s) 742, 800, 844, 862, 1507
BpiI GAAGAC 2 cut(s) 402, 1256
Bpu10I CCTNAGC 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 1703
BsaBI GATNNNNATC 1 cut(s) 1497
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 6 cut(s) 213, 239, 282, 1183, 1305, 1368
BsaWI WCCGGW 2 cut(s) 265, 712
BsaXI ACNNNNNCTCC 2 cut(s) 1301, 1331
Bsc4I CCNNNNNNNGG 4 cut(s) 240, 298, 719, 1274
Bse1I ACTGG 7 cut(s) 545, 566, 756, 858, 1268, 1447, 1478
Bse3DI GCAATG 1 cut(s) 820
Bse8I GATNNNNATC 1 cut(s) 1497
BseAI TCCGGA 1 cut(s) 712
BseBI CCWGG 4 cut(s) 190, 656, 795, 949
BseDI CCNNGG 6 cut(s) 213, 239, 282, 1183, 1305, 1368
BseJI GATNNNNATC 1 cut(s) 1497
BseLI CCNNNNNNNGG 4 cut(s) 240, 298, 719, 1274
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 5 cut(s) 172, 376, 732, 870, 1402
BseNI ACTGG 7 cut(s) 545, 566, 756, 858, 1268, 1447, 1478
BseRI GAGGAG 6 cut(s) 91, 103, 167, 170, 173, 1607
BseXI GCAGC 6 cut(s) 42, 563, 1001, 1542, 1545, 1548
BsgI GTGCAG 1 cut(s) 1549
BshFI GGCC 1 cut(s) 1457
BsiHKAI GWGCWC 2 cut(s) 345, 665
BsiSI CCGG 2 cut(s) 266, 713
BslFI GGGAC 1 cut(s) 1291
BslI CCNNNNNNNGG 4 cut(s) 240, 298, 719, 1274
BsmAI GTCTC 3 cut(s) 223, 267, 360
BsmFI GGGAC 1 cut(s) 1291
BsmI GAATGC 1 cut(s) 1016
BsnI GGCC 1 cut(s) 1457
Bso31I GGTCTC 1 cut(s) 223
Bsp1286I GDGCHC 2 cut(s) 345, 665
Bsp13I TCCGGA 1 cut(s) 712
Bsp143I GATC 5 cut(s) 328, 691, 1348, 1391, 1492
BspACI CCGC 8 cut(s) 21, 82, 195, 198, 201, 210, 413, 544
BspANI GGCC 1 cut(s) 1457
BspCNI CTCAG 5 cut(s) 171, 375, 731, 871, 1401
BspEI TCCGGA 1 cut(s) 712
BspMAI CTGCAG 1 cut(s) 1019
BspTNI GGTCTC 1 cut(s) 223
BsrBI CCGCTC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 820
BsrI ACTGG 7 cut(s) 545, 566, 756, 858, 1268, 1447, 1478
BssECI CCNNGG 6 cut(s) 213, 239, 282, 1183, 1305, 1368
BssMI GATC 5 cut(s) 328, 691, 1348, 1391, 1492
BssNAI GTATAC 1 cut(s) 1302
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 4 cut(s) 239, 282, 1183, 1305
Bst1107I GTATAC 1 cut(s) 1302
Bst2UI CCWGG 4 cut(s) 190, 656, 795, 949
Bst4CI ACNGT 2 cut(s) 151, 1369
Bst6I CTCTTC 4 cut(s) 92, 98, 431, 1045
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 480
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 6 cut(s) 158, 362, 509, 718, 879, 1388
BstDSI CCRYGG 2 cut(s) 213, 1368
BstKTI GATC 5 cut(s) 331, 694, 1351, 1394, 1495
BstMAI GTCTC 3 cut(s) 223, 267, 360
BstMBI GATC 5 cut(s) 328, 691, 1348, 1391, 1492
BstMWI GCNNNNNNNGC 6 cut(s) 206, 209, 480, 620, 660, 1536
BstNI CCWGG 4 cut(s) 190, 656, 795, 949
BstSCI CCNGG 4 cut(s) 188, 654, 793, 947
BstSFI CTRYAG 1 cut(s) 1015
BstV1I GCAGC 6 cut(s) 42, 563, 1001, 1542, 1545, 1548
BstV2I GAAGAC 2 cut(s) 402, 1256
BstX2I RGATCY 1 cut(s) 1391
BstYI RGATCY 1 cut(s) 1391
BstZ17I GTATAC 1 cut(s) 1302
BsuI GTATCC 1 cut(s) 1648
BsuRI GGCC 1 cut(s) 1457
BtgI CCRYGG 2 cut(s) 213, 1368
BtrI CACGTC 1 cut(s) 737
BtsIMutI CAGTG 3 cut(s) 763, 1440, 1539
Cac8I GCNNGC 1 cut(s) 309
Cfr13I GGNCC 5 cut(s) 192, 770, 1119, 1365, 1378
CseI GACGC 1 cut(s) 215
Csp6I GTAC 2 cut(s) 905, 1481
CviAII CATG 6 cut(s) 51, 235, 989, 1111, 1603, 1671
CviQI GTAC 2 cut(s) 905, 1481
DdeI CTNAG 6 cut(s) 158, 362, 509, 718, 879, 1388
DpnI GATC 5 cut(s) 330, 693, 1350, 1393, 1494
DpnII GATC 5 cut(s) 328, 691, 1348, 1391, 1492
DraI TTTAAA 1 cut(s) 1243
DriI GACNNNNNGTC 2 cut(s) 259, 501
EaeI YGGCCR 1 cut(s) 1455
Eam1104I CTCTTC 4 cut(s) 92, 98, 431, 1045
Eam1105I GACNNNNNGTC 2 cut(s) 259, 501
EarI CTCTTC 4 cut(s) 92, 98, 431, 1045
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 663
Eco130I CCWWGG 4 cut(s) 239, 282, 1183, 1305
Eco24I GRGCYC 1 cut(s) 665
Eco31I GGTCTC 1 cut(s) 223
Eco47I GGWCC 5 cut(s) 192, 770, 1119, 1365, 1378
Eco53kI GAGCTC 1 cut(s) 663
Eco57I CTGAAG 2 cut(s) 1026, 1563
EcoICRI GAGCTC 1 cut(s) 663
EcoO109I RGGNCCY 2 cut(s) 1119, 1378
EcoRI GAATTC 1 cut(s) 625
EcoRII CCWGG 4 cut(s) 188, 654, 793, 947
EcoT14I CCWWGG 4 cut(s) 239, 282, 1183, 1305
EcoT38I GRGCYC 1 cut(s) 665
ErhI CCWWGG 4 cut(s) 239, 282, 1183, 1305
FaeI CATG 6 cut(s) 54, 238, 992, 1114, 1606, 1674
FalI AAGNNNNNCTT 4 cut(s) 678, 710, 1289, 1321
FaqI GGGAC 1 cut(s) 1291
FatI CATG 6 cut(s) 50, 234, 988, 1110, 1602, 1670
FauI CCCGC 1 cut(s) 537
FbaI TGATCA 1 cut(s) 328
FblI GTMKAC 1 cut(s) 1301
Fnu4HI GCNGC 9 cut(s) 31, 198, 201, 210, 577, 1015, 1531, 1534, 1537
FriOI GRGCYC 1 cut(s) 665
Fsp4HI GCNGC 9 cut(s) 31, 198, 201, 210, 577, 1015, 1531, 1534, 1537
FspBI CTAG 3 cut(s) 240, 450, 1260
GluI GCNGC 9 cut(s) 31, 198, 201, 210, 577, 1015, 1531, 1534, 1537
HaeIII GGCC 1 cut(s) 1457
HapII CCGG 2 cut(s) 266, 713
HgaI GACGC 1 cut(s) 215
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 6 cut(s) 54, 238, 992, 1114, 1606, 1674
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 1 cut(s) 11
HpaII CCGG 2 cut(s) 266, 713
HphI GGTGA 6 cut(s) 139, 178, 383, 670, 773, 1019
Hpy166II GTNNAC 6 cut(s) 48, 154, 424, 463, 538, 1302
Hpy188III TCNNGA 3 cut(s) 713, 1097, 1294
Hpy8I GTNNAC 6 cut(s) 48, 154, 424, 463, 538, 1302
Hpy99I CGWCG 3 cut(s) 209, 257, 260
HpyAV CCTTC 2 cut(s) 323, 1677
HpyCH4III ACNGT 2 cut(s) 151, 1369
HpyCH4IV ACGT 1 cut(s) 736
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 209, 480, 620, 660, 1536
HpyF3I CTNAG 6 cut(s) 158, 362, 509, 718, 879, 1388
HpySE526I ACGT 1 cut(s) 736
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 6 cut(s) 54, 238, 992, 1114, 1606, 1674
Kpn2I TCCGGA 1 cut(s) 712
Ksp22I TGATCA 1 cut(s) 328
Kzo9I GATC 5 cut(s) 328, 691, 1348, 1391, 1492
LmnI GCTCC 2 cut(s) 796, 1187
Lsp1109I GCAGC 6 cut(s) 42, 563, 1001, 1542, 1545, 1548
LweI GCATC 5 cut(s) 742, 800, 844, 862, 1507
MaeI CTAG 3 cut(s) 240, 450, 1260
MaeII ACGT 1 cut(s) 736
MaeIII GTNAC 1 cut(s) 1573
MalI GATC 5 cut(s) 330, 693, 1350, 1393, 1494
MbiI CCGCTC 1 cut(s) 415
MboI GATC 5 cut(s) 328, 691, 1348, 1391, 1492
MflI RGATCY 1 cut(s) 1391
MhlI GDGCHC 2 cut(s) 345, 665
MlsI TGGCCA 1 cut(s) 1457
MluCI AATT 8 cut(s) 625, 707, 1154, 1352, 1407, 1426, 1550, 1625
MluNI TGGCCA 1 cut(s) 1457
MlyI GAGTC 5 cut(s) 269, 362, 419, 710, 788
MmeI TCCRAC 5 cut(s) 363, 679, 810, 1252, 1542
Mox20I TGGCCA 1 cut(s) 1457
MroI TCCGGA 1 cut(s) 712
MscI TGGCCA 1 cut(s) 1457
MseI TTAA 5 cut(s) 1242, 1374, 1419, 1553, 1628
MslI CAYNNNNRTG 4 cut(s) 518, 1511, 1669, 1709
Msp20I TGGCCA 1 cut(s) 1457
MspI CCGG 2 cut(s) 266, 713
MspR9I CCNGG 4 cut(s) 190, 656, 795, 949
Mva1269I GAATGC 1 cut(s) 1016
MvaI CCWGG 4 cut(s) 190, 656, 795, 949
MwoI GCNNNNNNNGC 6 cut(s) 206, 209, 480, 620, 660, 1536
NdeII GATC 5 cut(s) 328, 691, 1348, 1391, 1492
NlaIII CATG 6 cut(s) 54, 238, 992, 1114, 1606, 1674
OliI CACNNNNGTG 1 cut(s) 518
PctI GAATGC 1 cut(s) 1016
PfeI GAWTC 6 cut(s) 56, 746, 923, 944, 1060, 1568
PfoI TCCNGGA 2 cut(s) 793, 947
PkrI GCNGC 9 cut(s) 32, 199, 202, 211, 578, 1016, 1532, 1535, 1538
PleI GAGTC 5 cut(s) 268, 361, 419, 710, 787
PpsI GAGTC 5 cut(s) 268, 361, 419, 710, 787
PpuMI RGGWCCY 2 cut(s) 1119, 1378
PshBI ATTAAT 2 cut(s) 1419, 1628
Psp124BI GAGCTC 1 cut(s) 665
Psp5II RGGWCCY 2 cut(s) 1119, 1378
Psp6I CCWGG 4 cut(s) 188, 654, 793, 947
PspGI CCWGG 4 cut(s) 188, 654, 793, 947
PspPI GGNCC 5 cut(s) 192, 770, 1119, 1365, 1378
PspPPI RGGWCCY 2 cut(s) 1119, 1378
PstI CTGCAG 1 cut(s) 1019
PsuI RGATCY 1 cut(s) 1391
RsaI GTAC 2 cut(s) 906, 1482
RsaNI GTAC 2 cut(s) 905, 1481
RseI CAYNNNNRTG 4 cut(s) 518, 1511, 1669, 1709
SacI GAGCTC 1 cut(s) 665
SaqAI TTAA 5 cut(s) 1242, 1374, 1419, 1553, 1628
SatI GCNGC 9 cut(s) 31, 198, 201, 210, 577, 1015, 1531, 1534, 1537
Sau3AI GATC 5 cut(s) 328, 691, 1348, 1391, 1492
Sau96I GGNCC 5 cut(s) 192, 770, 1119, 1365, 1378
SchI GAGTC 5 cut(s) 269, 362, 419, 710, 788
ScrFI CCNGG 4 cut(s) 190, 656, 795, 949
SduI GDGCHC 2 cut(s) 345, 665
SfaNI GCATC 5 cut(s) 742, 800, 844, 862, 1507
SfcI CTRYAG 1 cut(s) 1015
SinI GGWCC 5 cut(s) 192, 770, 1119, 1365, 1378
SmiMI CAYNNNNRTG 4 cut(s) 518, 1511, 1669, 1709
SmlI CTYRAG 1 cut(s) 1682
SmoI CTYRAG 1 cut(s) 1682
SpeI ACTAGT 1 cut(s) 1259
Sse9I AATT 8 cut(s) 625, 707, 1154, 1352, 1407, 1426, 1550, 1625
SsiI CCGC 8 cut(s) 21, 82, 195, 198, 201, 210, 413, 544
SspI AATATT 2 cut(s) 1105, 1252
SspMI CTAG 3 cut(s) 240, 450, 1260
SstI GAGCTC 1 cut(s) 665
StyD4I CCNGG 4 cut(s) 188, 654, 793, 947
StyI CCWWGG 4 cut(s) 239, 282, 1183, 1305
TaaI ACNGT 2 cut(s) 151, 1369
TaiI ACGT 1 cut(s) 739
TaqI TCGA 2 cut(s) 252, 1234
TasI AATT 8 cut(s) 625, 707, 1154, 1352, 1407, 1426, 1550, 1625
TauI GCSGC 3 cut(s) 200, 203, 212
TfiI GAWTC 6 cut(s) 56, 746, 923, 944, 1060, 1568
Tru1I TTAA 5 cut(s) 1242, 1374, 1419, 1553, 1628
Tru9I TTAA 5 cut(s) 1242, 1374, 1419, 1553, 1628
TscAI CASTG 3 cut(s) 763, 1447, 1546
TseI GCWGC 6 cut(s) 30, 576, 1014, 1530, 1533, 1536
TspDTI ATGAA 9 cut(s) 402, 408, 759, 900, 1099, 1164, 1591, 1646, 1659
TspRI CASTG 3 cut(s) 763, 1447, 1546
VpaK11BI GGWCC 5 cut(s) 192, 770, 1119, 1365, 1378
VspI ATTAAT 2 cut(s) 1419, 1628
XapI RAATTY 4 cut(s) 625, 707, 1154, 1426
XcmI CCANNNNNNNNNTGG 1 cut(s) 537
XmaJI CCTAGG 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 1301
XspI CTAG 3 cut(s) 240, 450, 1260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.