Rmu_sc0001659.1_g000020

HECT-like Ubiquitin-conjugating enzyme (E2)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001659.1
OG0015450
Physical Location & Seq
Forward (+)
81988 .. 84845
2858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001659.1_g000020.1.cds

Sequence Viewer

Length: 1698 bp
atgagcttgaagcctgaaaccaccggcgaaactggtataaaatggcggttcacatgggaatcccaatcccacatcccaaccctccgcctattcctattcgattcctccaccaccccctccactcaatgccacgacctcaaggtccacctcagcctctcagactctcttgtcctgctgacctggaccgccgccggcgccggcgaagtctcgctcagggttcccatgcctagggttttactggacgacgagtcgccggtcagcttcaaagccttggatgaccacattgaggtcaagctcgccctccttctccctgttgatcactctattgtgctcaacttcgactctatactcagtttgagtgagatgaggttgaatggagacgaagagaatggagcgaaaccgctctcggtggactccgatgtaaagagtctatcttctgggggagaagttcacttttattgcagaagttgctcatataagttgactgcaagaccccttagaacttttgtggaaatgccttcagtcaactggcgagaggtggcggataactggtttgggaattgctgctgttcgtttggagaagttagtgagaagctggttgctggatatgcaaattcttacacaagtaagattggtgtgtgcctggtgagctctacaaatattaccctttgcaaagatgatcttgttgaatgtgagtttctcgaatcgggtgtatgtgaaagacaggataatgaatcagatgccagtggtgagtgtggtcctaatgaatctgaacttgctcctggaagtaatggcggatgtaatgagatacttaagtcagaaaataaggaaaagtatatagatgctgattgtaaaagtgacgctactaaggaagaaagtaacagtgagggtgtgcctcgtaggggttcagaatcggattgttctgtaagattgacatcagtagcagtatgctgtgacgatatagaaagtcatgtgcaaaactatgatggtgaaggctgcaggcatcatttgtctgaatcttctacagagcaaaatacggagattctgaaaaatcacaaatctttgctaaatgggtttcttgaaaatattttcatggttaggtcctccaatctttcagtagatgttgaatgggttgaatttttctgccctcagtgttcatctctccttggagcttacccttgtgataatggtggcagacttattgatggtggtgttcgattgtttaaatgcagtattgcaactagtcttccagtcggtgggacaagagacgttttcaggaagtataccttggagagaatgtttgcaaaccagctactcgaatgtgcaaaagatcagttatcatttaggaccgtggttagggacctgaaacaaagatctccaatgctgcaaattgttctcataaatacaaattcttggtccagcactggtcattgtctggccacacagggcaataccaaatcagtaccaaagattgatctgcatcctgtcatcaaggtgctattttccgagtgcagcagcagcactgagtctcaattaaggatgttggaagattcaataacaaaggatgtagctgaggaggttttcatgctgacacatcagatagaagaattaatcaagtctctgtcagcaagacaggatatacttccaccttcatactcatccctcgagggtttatctttgtcatccatgccaatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HECT_2

Protein Analysis

565

Amino Acids

62.37

Weight (kDa)

4.89

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26750
fragaria_vesca FvH4_1g12740
malus_domestica MD15G1254500.v1.1
prunus_persica Prupe.7G159100_v2.0.a1 Prupe.7G159100_v2.0.a1
pyrus_communis pycom15g22360
rosa_chinensis RchiOBHm_Chr2g0100811
rosa_laevigata RLG00000017013
rosa_multiflora Rmu_sc0001659.1_g000020
rosa_roxburghii Rroxscaffold_2G00141840
rosa_rugosa Rorug02G0093000
rosa_samantha Rh2AG140600 Rh2BG145600 Rh2CG146600 Rh2DG146000
rosa_wichuraiana Rw2G011000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 140, 167
AbsI CCTCGAGG 1 cut(s) 1664
AccB1I GGYRCC 1 cut(s) 194
AccB7I CCANNNNNTGG 1 cut(s) 1256
AccBSI CCGCTC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 1283
AciI CCGC 7 cut(s) 46, 85, 186, 189, 401, 542, 795
AcoI YGGCCR 1 cut(s) 1437
AcsI RAATTY 3 cut(s) 613, 1136, 1408
AcuI CTGAAG 1 cut(s) 504
AcyI GRCGYC 1 cut(s) 195
AfaI GTAC 1 cut(s) 1464
AfiI CCNNNNNNNGG 5 cut(s) 228, 286, 902, 1256, 1356
AflII CTTAAG 1 cut(s) 812
AgsI TTSAA 8 cut(s) 10, 265, 373, 689, 1082, 1127, 1136, 1554
AhdI GACNNNNNGTC 1 cut(s) 247
AhlI ACTAGT 1 cut(s) 1241
AjnI CCWGG 3 cut(s) 179, 642, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 1271, 1303
AluBI AGCT 8 cut(s) 6, 261, 295, 595, 651, 1172, 1312, 1571
AluI AGCT 8 cut(s) 6, 261, 295, 595, 651, 1172, 1312, 1571
Alw21I GWGCWC 2 cut(s) 333, 653
Alw26I GTCTC 5 cut(s) 211, 372, 1260, 1533, 1623
Ama87I CYCGRG 1 cut(s) 1664
AoxI GGCC 1 cut(s) 1437
ApeKI GCWGC 6 cut(s) 564, 996, 1384, 1512, 1515, 1518
ApoI RAATTY 3 cut(s) 613, 1136, 1408
ArsI GACNNNNNNTTYG 2 cut(s) 1400, 1432
AseI ATTAAT 1 cut(s) 1610
AspA2I CCTAGG 1 cut(s) 227
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
AsuHPI GGTGA 3 cut(s) 658, 761, 1001
AvaI CYCGRG 1 cut(s) 1664
AvaII GGWCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
AvrII CCTAGG 1 cut(s) 227
BaeI ACNNNNGTAYC 2 cut(s) 800, 833
BalI TGGCCA 1 cut(s) 1439
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 653
BbsI GAAGAC 1 cut(s) 1238
Bbv12I GWGCWC 2 cut(s) 333, 653
BbvCI CCTCAGC 2 cut(s) 149, 1572
BbvI GCAGC 6 cut(s) 551, 983, 1371, 1524, 1527, 1530
BccI CCATC 2 cut(s) 980, 1199
BciT130I CCWGG 3 cut(s) 181, 644, 783
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 5 cut(s) 211, 372, 1260, 1533, 1623
BcuI ACTAGT 1 cut(s) 1241
BfaI CTAG 2 cut(s) 228, 1242
BfmI CTRYAG 2 cut(s) 997, 1023
BfoI RGCGCY 1 cut(s) 198
BfrI CTTAAG 1 cut(s) 812
BglII AGATCT 1 cut(s) 1373
BisI GCNGC 7 cut(s) 189, 565, 997, 1385, 1513, 1516, 1519
BlnI CCTAGG 1 cut(s) 227
BlsI GCNGC 7 cut(s) 190, 566, 998, 1386, 1514, 1517, 1520
Bme1390I CCNGG 3 cut(s) 181, 644, 783
Bme18I GGWCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
BmeRI GACNNNNNGTC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 1664
BmgT120I GGNCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
BmiI GGNNCC 3 cut(s) 196, 219, 1361
BmrFI CCNGG 3 cut(s) 181, 644, 783
BmsI GCATC 4 cut(s) 730, 832, 1012, 1489
BpiI GAAGAC 1 cut(s) 1238
Bpu10I CCTNAGC 3 cut(s) 149, 212, 1572
BpuEI CTTGAG 1 cut(s) 122
BsaBI GATNNNNATC 2 cut(s) 934, 1479
BsaHI GRCGYC 1 cut(s) 195
BsaJI CCNNGG 5 cut(s) 227, 270, 1165, 1287, 1350
BsaXI ACNNNNNCTCC 2 cut(s) 1283, 1313
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 286, 902, 1256, 1356
Bse118I RCCGGY 4 cut(s) 23, 191, 197, 253
Bse1I ACTGG 7 cut(s) 37, 243, 533, 554, 744, 1250, 1429
Bse8I GATNNNNATC 2 cut(s) 934, 1479
BseBI CCWGG 3 cut(s) 181, 644, 783
BseDI CCNNGG 5 cut(s) 227, 270, 1165, 1287, 1350
BseGI GGATG 8 cut(s) 72, 280, 803, 1480, 1545, 1570, 1658, 1682
BseJI GATNNNNATC 2 cut(s) 934, 1479
BseLI CCNNNNNNNGG 5 cut(s) 228, 286, 902, 1256, 1356
BseMII CTCAG 7 cut(s) 163, 171, 226, 364, 1163, 1515, 1563
BseNI ACTGG 7 cut(s) 37, 243, 533, 554, 744, 1250, 1429
BseRI GAGGAG 1 cut(s) 1589
BseXI GCAGC 6 cut(s) 551, 983, 1371, 1524, 1527, 1530
BsgI GTGCAG 1 cut(s) 1531
BshFI GGCC 1 cut(s) 1439
BshNI GGYRCC 1 cut(s) 194
BsiHKAI GWGCWC 2 cut(s) 333, 653
BsiHKCI CYCGRG 1 cut(s) 1664
BsiSI CCGG 4 cut(s) 24, 192, 198, 254
BslFI GGGAC 2 cut(s) 1273, 1373
BslI CCNNNNNNNGG 5 cut(s) 228, 286, 902, 1256, 1356
BsmAI GTCTC 5 cut(s) 211, 372, 1260, 1533, 1623
BsmBI CGTCTC 2 cut(s) 372, 1260
BsmFI GGGAC 2 cut(s) 1273, 1373
BsnI GGCC 1 cut(s) 1439
BsoBI CYCGRG 1 cut(s) 1664
Bsp1286I GDGCHC 2 cut(s) 333, 653
Bsp143I GATC 5 cut(s) 316, 679, 1330, 1373, 1474
BspACI CCGC 7 cut(s) 46, 85, 186, 189, 401, 542, 795
BspANI GGCC 1 cut(s) 1439
BspCNI CTCAG 7 cut(s) 162, 170, 225, 363, 1162, 1516, 1564
BspLI GGNNCC 3 cut(s) 196, 219, 1361
BspMAI CTGCAG 1 cut(s) 1001
BspT107I GGYRCC 1 cut(s) 194
BspTI CTTAAG 1 cut(s) 812
BsrBI CCGCTC 1 cut(s) 403
BsrFI RCCGGY 4 cut(s) 23, 191, 197, 253
BsrI ACTGG 7 cut(s) 37, 243, 533, 554, 744, 1250, 1429
BssAI RCCGGY 4 cut(s) 23, 191, 197, 253
BssECI CCNNGG 5 cut(s) 227, 270, 1165, 1287, 1350
BssMI GATC 5 cut(s) 316, 679, 1330, 1373, 1474
BssNAI GTATAC 1 cut(s) 1284
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 4 cut(s) 227, 270, 1165, 1287
Bst1107I GTATAC 1 cut(s) 1284
Bst2UI CCWGG 3 cut(s) 181, 644, 783
Bst4CI ACNGT 2 cut(s) 884, 1351
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 195
BstAFI CTTAAG 1 cut(s) 812
BstAPI GCANNNNNTGC 1 cut(s) 468
BstC8I GCNNGC 4 cut(s) 193, 199, 297, 1001
BstDEI CTNAG 9 cut(s) 149, 157, 212, 350, 497, 867, 1149, 1524, 1572
BstDSI CCRYGG 1 cut(s) 1350
BstF5I GGATG 8 cut(s) 72, 280, 803, 1480, 1545, 1570, 1658, 1682
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 5 cut(s) 319, 682, 1333, 1376, 1477
BstMAI GTCTC 5 cut(s) 211, 372, 1260, 1533, 1623
BstMBI GATC 5 cut(s) 316, 679, 1330, 1373, 1474
BstMWI GCNNNNNNNGC 5 cut(s) 194, 468, 608, 648, 1518
BstNI CCWGG 3 cut(s) 181, 644, 783
BstSCI CCNGG 3 cut(s) 179, 642, 781
BstSFI CTRYAG 2 cut(s) 997, 1023
BstV1I GCAGC 6 cut(s) 551, 983, 1371, 1524, 1527, 1530
BstV2I GAAGAC 1 cut(s) 1238
BstX2I RGATCY 1 cut(s) 1373
BstYI RGATCY 1 cut(s) 1373
BstZ17I GTATAC 1 cut(s) 1284
BsuRI GGCC 1 cut(s) 1439
BtgI CCRYGG 1 cut(s) 1350
BtsCI GGATG 8 cut(s) 72, 280, 803, 1480, 1545, 1570, 1658, 1682
BtsIMutI CAGTG 5 cut(s) 751, 889, 1157, 1422, 1521
Cac8I GCNNGC 4 cut(s) 193, 199, 297, 1001
CfoI GCGC 1 cut(s) 197
Cfr10I RCCGGY 4 cut(s) 23, 191, 197, 253
Cfr13I GGNCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
CseI GACGC 1 cut(s) 869
Csp6I GTAC 1 cut(s) 1463
CviAII CATG 6 cut(s) 54, 223, 971, 1093, 1585, 1687
CviQI GTAC 1 cut(s) 1463
DdeI CTNAG 9 cut(s) 149, 157, 212, 350, 497, 867, 1149, 1524, 1572
DinI GGCGCC 1 cut(s) 196
DpnI GATC 5 cut(s) 318, 681, 1332, 1375, 1476
DpnII GATC 5 cut(s) 316, 679, 1330, 1373, 1474
DraI TTTAAA 1 cut(s) 1225
DrdI GACNNNNNNGTC 2 cut(s) 140, 167
DriI GACNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 2 cut(s) 140, 167
EaeI YGGCCR 1 cut(s) 1437
Eam1104I CTCTTC 1 cut(s) 378
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 3 cut(s) 74, 557, 810
Ecl136II GAGCTC 1 cut(s) 651
Eco130I CCWWGG 4 cut(s) 227, 270, 1165, 1287
Eco24I GRGCYC 1 cut(s) 653
Eco47I GGWCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
Eco53kI GAGCTC 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 1664
EcoICRI GAGCTC 1 cut(s) 651
EcoO109I RGGNCCY 2 cut(s) 1101, 1360
EcoRII CCWGG 3 cut(s) 179, 642, 781
EcoT14I CCWWGG 4 cut(s) 227, 270, 1165, 1287
EcoT38I GRGCYC 1 cut(s) 653
EgeI GGCGCC 1 cut(s) 196
EheI GGCGCC 1 cut(s) 196
ErhI CCWWGG 4 cut(s) 227, 270, 1165, 1287
Esp3I CGTCTC 2 cut(s) 372, 1260
FaeI CATG 6 cut(s) 57, 226, 974, 1096, 1588, 1690
FalI AAGNNNNNCTT 4 cut(s) 666, 698, 1271, 1303
FaqI GGGAC 2 cut(s) 1273, 1373
FatI CATG 6 cut(s) 53, 222, 970, 1092, 1584, 1686
FbaI TGATCA 1 cut(s) 316
FblI GTMKAC 1 cut(s) 1283
Fnu4HI GCNGC 7 cut(s) 189, 565, 997, 1385, 1513, 1516, 1519
FokI GGATG 8 cut(s) 59, 287, 810, 1467, 1552, 1577, 1645, 1669
FriOI GRGCYC 1 cut(s) 653
Fsp4HI GCNGC 7 cut(s) 189, 565, 997, 1385, 1513, 1516, 1519
FspBI CTAG 2 cut(s) 228, 1242
GlaI GCGC 1 cut(s) 196
GluI GCNGC 7 cut(s) 189, 565, 997, 1385, 1513, 1516, 1519
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 1 cut(s) 1439
HapII CCGG 4 cut(s) 24, 192, 198, 254
HgaI GACGC 1 cut(s) 869
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 6 cut(s) 57, 226, 974, 1096, 1588, 1690
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HincII GTYRAC 2 cut(s) 483, 526
HindII GTYRAC 2 cut(s) 483, 526
HpaII CCGG 4 cut(s) 24, 192, 198, 254
HphI GGTGA 3 cut(s) 658, 761, 1001
Hpy166II GTNNAC 7 cut(s) 51, 145, 412, 451, 483, 526, 1284
Hpy188III TCNNGA 3 cut(s) 701, 1079, 1276
Hpy8I GTNNAC 7 cut(s) 51, 145, 412, 451, 483, 526, 1284
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 4 cut(s) 314, 528, 986, 1659
HpyCH4III ACNGT 2 cut(s) 884, 1351
HpyCH4IV ACGT 1 cut(s) 1269
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 468, 608, 648, 1518
HpyF3I CTNAG 9 cut(s) 149, 157, 212, 350, 497, 867, 1149, 1524, 1572
HpySE526I ACGT 1 cut(s) 1269
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 6 cut(s) 57, 226, 974, 1096, 1588, 1690
HspAI GCGC 1 cut(s) 195
KasI GGCGCC 1 cut(s) 194
KroI GCCGGC 2 cut(s) 191, 197
KroNI GCCGGC 2 cut(s) 193, 199
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 5 cut(s) 316, 679, 1330, 1373, 1474
LmnI GCTCC 3 cut(s) 392, 784, 1169
Lsp1109I GCAGC 6 cut(s) 551, 983, 1371, 1524, 1527, 1530
LweI GCATC 4 cut(s) 730, 832, 1012, 1489
MaeI CTAG 2 cut(s) 228, 1242
MaeII ACGT 1 cut(s) 1269
MaeIII GTNAC 3 cut(s) 857, 878, 953
MalI GATC 5 cut(s) 318, 681, 1332, 1375, 1476
MbiI CCGCTC 1 cut(s) 403
MboI GATC 5 cut(s) 316, 679, 1330, 1373, 1474
MboII GAAGA 7 cut(s) 395, 426, 884, 1011, 1238, 1559, 1616
MflI RGATCY 1 cut(s) 1373
MhlI GDGCHC 2 cut(s) 333, 653
MlsI TGGCCA 1 cut(s) 1439
MluCI AATT 7 cut(s) 559, 613, 1136, 1389, 1408, 1532, 1607
MluNI TGGCCA 1 cut(s) 1439
Mly113I GGCGCC 1 cut(s) 195
MlyI GAGTC 6 cut(s) 155, 257, 335, 407, 436, 1535
MmeI TCCRAC 1 cut(s) 1524
Mox20I TGGCCA 1 cut(s) 1439
MreI CGCCGGCG 2 cut(s) 191, 197
MroNI GCCGGC 2 cut(s) 191, 197
MscI TGGCCA 1 cut(s) 1439
MseI TTAA 4 cut(s) 813, 1224, 1535, 1610
MslI CAYNNNNRTG 2 cut(s) 1493, 1691
Msp20I TGGCCA 1 cut(s) 1439
MspCI CTTAAG 1 cut(s) 812
MspI CCGG 4 cut(s) 24, 192, 198, 254
MspR9I CCNGG 3 cut(s) 181, 644, 783
MvaI CCWGG 3 cut(s) 181, 644, 783
MwoI GCNNNNNNNGC 5 cut(s) 194, 468, 608, 648, 1518
NaeI GCCGGC 2 cut(s) 193, 199
NarI GGCGCC 1 cut(s) 195
NdeII GATC 5 cut(s) 316, 679, 1330, 1373, 1474
NgoMIV GCCGGC 2 cut(s) 191, 197
NlaIII CATG 6 cut(s) 57, 226, 974, 1096, 1588, 1690
NlaIV GGNNCC 3 cut(s) 196, 219, 1361
NmuCI GTSAC 2 cut(s) 857, 953
PaeR7I CTCGAG 1 cut(s) 1664
PdiI GCCGGC 2 cut(s) 193, 199
PfeI GAWTC 9 cut(s) 59, 101, 704, 734, 767, 911, 1016, 1042, 1550
PflMI CCANNNNNTGG 1 cut(s) 1256
PfoI TCCNGGA 1 cut(s) 781
PkrI GCNGC 7 cut(s) 190, 566, 998, 1386, 1514, 1517, 1520
PleI GAGTC 6 cut(s) 155, 256, 335, 407, 435, 1534
PluTI GGCGCC 1 cut(s) 198
PpsI GAGTC 6 cut(s) 155, 256, 335, 407, 435, 1534
PpuMI RGGWCCY 2 cut(s) 1101, 1360
PshBI ATTAAT 1 cut(s) 1610
Psp124BI GAGCTC 1 cut(s) 653
Psp5II RGGWCCY 2 cut(s) 1101, 1360
Psp6I CCWGG 3 cut(s) 179, 642, 781
PspGI CCWGG 3 cut(s) 179, 642, 781
PspN4I GGNNCC 3 cut(s) 196, 219, 1361
PspPI GGNCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
PspPPI RGGWCCY 2 cut(s) 1101, 1360
PspXI VCTCGAGB 1 cut(s) 1664
PstI CTGCAG 1 cut(s) 1001
PsuI RGATCY 1 cut(s) 1373
RsaI GTAC 1 cut(s) 1464
RsaNI GTAC 1 cut(s) 1463
RseI CAYNNNNRTG 2 cut(s) 1493, 1691
SacI GAGCTC 1 cut(s) 653
SaqAI TTAA 4 cut(s) 813, 1224, 1535, 1610
SatI GCNGC 7 cut(s) 189, 565, 997, 1385, 1513, 1516, 1519
Sau3AI GATC 5 cut(s) 316, 679, 1330, 1373, 1474
Sau96I GGNCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
SchI GAGTC 6 cut(s) 155, 257, 335, 407, 436, 1535
ScrFI CCNGG 3 cut(s) 181, 644, 783
SduI GDGCHC 2 cut(s) 333, 653
SfaNI GCATC 4 cut(s) 730, 832, 1012, 1489
SfcI CTRYAG 2 cut(s) 997, 1023
SfoI GGCGCC 1 cut(s) 196
Sfr274I CTCGAG 1 cut(s) 1664
SgrAI CRCCGGYG 3 cut(s) 23, 191, 197
SinI GGWCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
SlaI CTCGAG 1 cut(s) 1664
SmiMI CAYNNNNRTG 2 cut(s) 1493, 1691
SmlI CTYRAG 3 cut(s) 137, 812, 1664
SmoI CTYRAG 3 cut(s) 137, 812, 1664
SpeI ACTAGT 1 cut(s) 1241
Sse9I AATT 7 cut(s) 559, 613, 1136, 1389, 1408, 1532, 1607
SsiI CCGC 7 cut(s) 46, 85, 186, 189, 401, 542, 795
SspDI GGCGCC 1 cut(s) 194
SspI AATATT 2 cut(s) 661, 1087
SspMI CTAG 2 cut(s) 228, 1242
SstI GAGCTC 1 cut(s) 653
StyD4I CCNGG 3 cut(s) 179, 642, 781
StyI CCWWGG 4 cut(s) 227, 270, 1165, 1287
TaaI ACNGT 2 cut(s) 884, 1351
TaiI ACGT 1 cut(s) 1272
TaqI TCGA 6 cut(s) 99, 339, 702, 1216, 1317, 1665
TasI AATT 7 cut(s) 559, 613, 1136, 1389, 1408, 1532, 1607
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 9 cut(s) 59, 101, 704, 734, 767, 911, 1016, 1042, 1550
Tru1I TTAA 4 cut(s) 813, 1224, 1535, 1610
Tru9I TTAA 4 cut(s) 813, 1224, 1535, 1610
TscAI CASTG 5 cut(s) 751, 889, 1157, 1429, 1528
TseFI GTSAC 2 cut(s) 857, 953
TseI GCWGC 6 cut(s) 564, 996, 1384, 1512, 1515, 1518
Tsp45I GTSAC 2 cut(s) 857, 953
TspDTI ATGAA 6 cut(s) 747, 780, 1081, 1146, 1573, 1641
TspGWI ACGGA 1 cut(s) 1052
TspRI CASTG 5 cut(s) 751, 889, 1157, 1429, 1528
Van91I CCANNNNNTGG 1 cut(s) 1256
Vha464I CTTAAG 1 cut(s) 812
VpaK11BI GGWCC 7 cut(s) 142, 183, 758, 1101, 1347, 1360, 1416
VspI ATTAAT 1 cut(s) 1610
XapI RAATTY 3 cut(s) 613, 1136, 1408
XhoI CTCGAG 1 cut(s) 1664
XmaJI CCTAGG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 1283
XspI CTAG 2 cut(s) 228, 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.