AT3G27325

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005447
Physical Location & Seq
Forward (+)
10095015 .. 10102423
7409 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27325.3

Sequence Viewer

Length: 3258 bp
ATGCGTGATGTAAGATTTGCTATGAGCATTCGACCAAGGCTGAGGATAGTTGGCGTTTTGATTTTAGCGGCATGGATTGGGCTTGTGGCTTTGTTTGGTTTGCTAAAACCTATAAAGAATGGTTGTACTATGACTTACATGTATCCTACTTACATTCCTATCTCTGTGACGGATGATACAACTCCTGGAAGATATGGACTTTATCTTTACCATGAAGGTTGGAGGAAGATTGACTTTAAGGAACACCTTAAGAAACTTAGTGGAGTTCCTGTTCTTTTCATTCCAGGCAATGCTGGTAGCTACAAGCAGGTCAGGTCTGTGGCAGCAGAATCTGATAGAGCGTTTCAGGGGGGCCCATTTGAGCGCACATTTTACCAGGAAGCTTCTCTACTCCGTGGGGGAGGTGCAGATACGGAGTCTGTAGACTATGATTTGCCTAGTCAGTACAGTAACAGACTAGATTGGTTTGCTGTGGATCTTGAAGGTGAACATTCTGCAATGGATGGTCGCATACTTGAGGAGCACACTGAATATGTCGTATATGCCATTCATAGGATTCTAGATCAATATAAGGAATCTCATGATACCAGAGAAAGAGAAGGTGCCGCTGCGTCCAGTAAATTACCACATGATGTGATCTTGGTTGGGCATTCTATGGGTGGTTTTGTTGCTAGAGCTGCTGCAGTCCATCCTCGTTTGCGGAAATCAGCTGTGCAAACCATTCTAACACTCTCAAGTCCACACCAATCACCTCCTCTGGCATTGCAGCCATCCTTGGGGCATTACTTTGCTCAAGTGAATCGAGAATGGAAAAAAGGTTATGAAGTTCAAACATCCCCTGGGGGAAATTATGTGTCTGACCCATTACTATCCGGAGTTGTTGTTGTATCCATTTCCGGTGGCTACAATGACTATCAGGTTCGATCGAAGTTAGAGTCACTTGATGGCATTGTCCCTTCAAGTCACGGCTTTATGATAAGTAGTACAAGCATGACAAACGTGTGGCTGTCAATGGAACATCAAGCTATTTTGTGGTGTAACCAATTGGTTGTTCAAGTTTCACATACGCTTCTTAGTATGGTTGACTCGAAAACTAATCAGCCATTTTCTGATACTGATAAGAGACTTTGGGTTTTAACCAGAATGCTTCAAAGTGCATTAGCTCAAAGTTTCAATGGAATGACACCCATGGAAGTCTCTCATGAGTTACCCATATTGGCCTCAAAGGGCTCTACGTGTTTCCTGGATTGGAGGGATGATGCTCTCGATAGGGATCTATACATACAAACAAGTACTGTCACGATTTTGGCAATGGACGGGAGAAGGCGCTGGTTGGACATAGATTTATTGGGGTCAAATGGAAAGAATCACTTTATATTTGTCACAAATCTGGTGCCTTGTTCTGGAGTAAGACTCCATCTATGGCCTGAGAAGGAGAAATCGAATTCAAATTTACCGGTTTGTGAAAGGGTCTTGGAAGTTACATCAAAGATGGTTCTCATTCCGGCAGGCCCTGCACCAAAACAGTCGGAACCAGGGAGTCAGACTGAACAAGCTCCCCCATCTGCAGTACTGAAGCTGGGGCCTGAAGACATGCGTGGATTCAGATTCTTGACTATCTCAGTTGCGCCTCGCGAAGCCGTTTCTGGTAAACCTCCAGTAGCAGTTTCCATGGCAGTAGGGCAGTTCTTTAATCCCGGAGACGGGGCCGTGGAGGTCTCATCTCAATCAATGCTCTTATCCACTTACTGGGCAAAGGAAATATTTTTGAAGGAGGACCATCCTCTAGCTTACAATCTATCGTTTTCTACCAGCTTAGGTCTATTGCCCGCAACACTGTCCCTGAAAACTACTGGATGCGGAATTAAAACTTTTGGTTTACCAGACGGCGAGACAGGAGACTTGGACAAGGATAAGCTCTGCAAATTGCGTTGTTTCCCACCTGTTGCTCTTGCCTGGGATTCTGCTTCTGGACTTCACGTTTTTGCAAATCTATACAGTGAGACAATAGTTATTGATTCATCACCAGCTCTTTGGAGTTCTCAGAGTTCTGAGAAAACAACCGTTATGCTATTGGTTGACCCTCATTGCTCATACACCGCAAGTGTTCATGTTTCTGCTCCAGCCATGTCCAGCAGATTTGTCCTTCTATATGGTCCTCAGATAGTTGGCTTCTCATTTGCCGTCATAATGTTCGCTCTTATGCGGCAAGCTAATCAATGGGACAACAAATTATCAGTGCCACCATTACTTTCTGCTGTAGAGTACAATTTAGAAATGCCATCCCCATTTCTGCTTCTAGCTGTTTTACCTCTTATAAGTTCATTGTTCTACTCCTTCCTAATGGGTCAACCAATTCCTCCCCTCACAAGCTTCACAGTGGTCTCGTTGATCTGTTATTTGTTGGCCAATGCCTTCATCAGTGTGTTAACTATTGTTTCCAAATTTCCCTTCCAAGCGTCGGCCCTTGTCCACACCACTGTTAAATCAAGGTGCCAAGCTTTGGAAAGAAACTATTCTCTTGCTTTCCTTCATTGGTTTTCTATACTTTCTTCCAGTTTCTTCTGTTTAAAGGCAATCCGAATTCTGAAGCTCAACACAACAATTCTTATGACACTAATCGCAGTTACCTTGGTGTCCTTTGTTCATCCGGCTTTGGGTCTTTTTGTACTCCTCGCCTCTCATGCCCTTTGCTGCCATAACTCCATGTGCTGTATTATGATGGCATCAAAGAGAAAGGAATCACTCGACCAGAAAAATGAAGCCGAGCGTAAAACGCGACATCCCTCAAGCAGAGAGGATCCCGTGTCAGGAGACTTGTCTGAAAAGTCATTTGTTGAGACGCAAGCAGACATCTTCAACCACAGGCACGGTTTACTAATATTGCATCTCCTTGCAGCTTTGATGTTCGTACCCTCTCTTGCCGCTTGGTTCCAGCGGATAGGAACAGGACAAAGCTTCCCTTGGTTTGCTGACTCGGCTCTCTGCGTTGGCGTAATCTTCCATGGAATACTGAACTCGAGACCCGAGTCAAGCATTCTACGCTCTTTCCCGTTTTTCTCAGGACACCAAATCCGTCCCCATCACATCTATCTTCTCGCTGGCTACTACTGCTTCTTCTCCGGGCTAGAGTTGGCTCCATACAAAGTGTTCTATGCGATAGCTTCTTTAGGCTATATCTCTTTGACTCTTAAGATCTCACAGGTGAACAAGAACGACCTTCGTTTCCGTACTAAAAGCAGAATACACAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

1085

Amino Acids

120.37

Weight (kDa)

8.45

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGAP1 PF07819 122 - 396 3.6e-85 PGAP1-like alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2318
Acc36I ACCTGC 1 cut(s) 298
AccB1I GGYRCC 3 cut(s) 602, 1393, 2493
AccB7I CCANNNNNTGG 2 cut(s) 1749, 2503
AccI GTMKAC 1 cut(s) 423
AccII CGCG 2 cut(s) 1635, 2779
AccIII TCCGGA 1 cut(s) 872
AciI CCGC 9 cut(s) 68, 606, 700, 1830, 1860, 2100, 2206, 2925, 2938
AclWI GGATC 4 cut(s) 483, 1281, 2795, 2808
AcoI YGGCCR 1 cut(s) 2406
AcsI RAATTY 4 cut(s) 1444, 1450, 2444, 2583
AcuI CTGAAG 3 cut(s) 1595, 1608, 2609
AfaI GTAC 9 cut(s) 127, 446, 985, 1294, 1572, 2267, 2670, 2913, 3232
AflII CTTAAG 2 cut(s) 248, 3191
AflIII ACRYGT 3 cut(s) 138, 999, 1235
AgeI ACCGGT 1 cut(s) 1456
AgsI TTSAA 9 cut(s) 482, 830, 960, 1055, 1151, 1174, 1449, 1771, 2860
AjnI CCWGG 7 cut(s) 184, 283, 375, 838, 1242, 1534, 1955
AjuI GAANNNNNNNTTGG 4 cut(s) 1035, 1067, 2435, 2467
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
Alw21I GWGCWC 1 cut(s) 525
AlwI GGATC 4 cut(s) 483, 1281, 2795, 2808
AlwNI CAGNNNCTG 3 cut(s) 332, 1514, 3255
Ama87I CYCGRG 2 cut(s) 3019, 3026
Aor13HI TCCGGA 1 cut(s) 872
AoxI GGCC 8 cut(s) 352, 1218, 1424, 1510, 1583, 1707, 2406, 2463
ApaI GGGCCC 1 cut(s) 356
ApeKI GCWGC 7 cut(s) 323, 608, 677, 680, 766, 2694, 2897
ApoI RAATTY 4 cut(s) 1444, 1450, 2444, 2583
ArsI GACNNNNNNTTYG 2 cut(s) 920, 952
AsiGI ACCGGT 1 cut(s) 1456
Asp700I GAANNNNTTC 1 cut(s) 2515
AspLEI GCGC 3 cut(s) 366, 1329, 1630
AspS9I GGNCC 8 cut(s) 352, 353, 1511, 1583, 1707, 1777, 2156, 2464
AsuC2I CCSGG 2 cut(s) 1698, 3124
AsuHPI GGTGA 4 cut(s) 497, 741, 2016, 3217
AvaI CYCGRG 2 cut(s) 3019, 3026
AvaII GGWCC 2 cut(s) 1777, 2156
BaeGI GKGCMC 1 cut(s) 356
BalI TGGCCA 1 cut(s) 2408
BamHI GGATCC 1 cut(s) 2800
BanI GGYRCC 3 cut(s) 602, 1393, 2493
BanII GRGCYC 2 cut(s) 356, 1232
BarI GAAGNNNNNNTAC 2 cut(s) 372, 404
BbsI GAAGAC 1 cut(s) 1596
Bbv12I GWGCWC 1 cut(s) 525
BbvCI CCTCAGC 1 cut(s) 41
BbvI GCAGC 7 cut(s) 335, 595, 664, 667, 778, 2681, 2909
BceAI ACGGC 5 cut(s) 982, 1625, 1694, 1903, 2168
BciT130I CCWGG 7 cut(s) 186, 285, 377, 840, 1244, 1536, 1957
BciVI GTATCC 2 cut(s) 153, 898
BcnI CCSGG 2 cut(s) 1698, 3124
BfaI CTAG 7 cut(s) 438, 458, 560, 672, 1787, 2300, 3128
BfmI CTRYAG 4 cut(s) 420, 681, 1566, 2259
BfoI RGCGCY 1 cut(s) 1330
BfrI CTTAAG 2 cut(s) 248, 3191
BfuAI ACCTGC 1 cut(s) 298
BfuI GTATCC 2 cut(s) 153, 898
BglII AGATCT 1 cut(s) 3195
BmcAI AGTACT 2 cut(s) 1294, 1572
Bme1390I CCNGG 9 cut(s) 186, 285, 377, 840, 1244, 1536, 1698, 1957, 3124
Bme18I GGWCC 2 cut(s) 1777, 2156
BmeT110I CYCGRG 2 cut(s) 3019, 3026
BmgT120I GGNCC 8 cut(s) 352, 353, 1511, 1583, 1707, 1777, 2156, 2464
BmrFI CCNGG 9 cut(s) 186, 285, 377, 840, 1244, 1536, 1698, 1957, 3124
BmrI ACTGGG 1 cut(s) 1759
BmsI GCATC 4 cut(s) 1249, 1847, 2735, 2896
BmuI ACTGGG 1 cut(s) 1759
BoxI GACNNNNGTC 1 cut(s) 3028
BpiI GAAGAC 1 cut(s) 1596
BplI GAGNNNNNCTC 2 cut(s) 1398, 1430
BpmI CTGGAG 3 cut(s) 1425, 1641, 2106
Bpu10I CCTNAGC 2 cut(s) 41, 1816
BpuEI CTTGAG 4 cut(s) 536, 718, 777, 2773
BpuMI CCSGG 2 cut(s) 1698, 3124
BsaAI YACGTR 1 cut(s) 1236
BsaBI GATNNNNATC 1 cut(s) 1272
BsaI GGTCTC 3 cut(s) 1723, 2389, 3016
BsaWI WCCGGW 3 cut(s) 872, 896, 1456
BsaXI ACNNNNNCTCC 4 cut(s) 255, 285, 867, 897
Bse118I RCCGGY 1 cut(s) 1456
Bse1I ACTGG 5 cut(s) 615, 1658, 1754, 1858, 2556
Bse3DI GCAATG 5 cut(s) 295, 504, 761, 1317, 2086
Bse8I GATNNNNATC 1 cut(s) 1272
BseAI TCCGGA 1 cut(s) 872
BseBI CCWGG 7 cut(s) 186, 285, 377, 840, 1244, 1536, 1957
BseJI GATNNNNATC 1 cut(s) 1272
BseMI GCAATG 5 cut(s) 295, 504, 761, 1317, 2086
BseMII CTCAG 7 cut(s) 32, 1419, 1635, 2043, 2057, 2174, 3075
BseNI ACTGG 5 cut(s) 615, 1658, 1754, 1858, 2556
BseRI GAGGAG 3 cut(s) 533, 744, 2663
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 7 cut(s) 335, 595, 664, 667, 778, 2681, 2909
BseYI CCCAGC 1 cut(s) 1579
BsgI GTGCAG 2 cut(s) 426, 1500
Bsh1236I CGCG 2 cut(s) 1635, 2779
Bsh1285I CGRYCG 1 cut(s) 926
BshFI GGCC 8 cut(s) 354, 1220, 1426, 1512, 1585, 1709, 2408, 2465
BshNI GGYRCC 3 cut(s) 602, 1393, 2493
BshTI ACCGGT 1 cut(s) 1456
BsiEI CGRYCG 1 cut(s) 926
BsiHKAI GWGCWC 1 cut(s) 525
BsiHKCI CYCGRG 2 cut(s) 3019, 3026
BsiSI CCGG 7 cut(s) 873, 897, 1457, 1505, 1698, 2651, 3123
BslFI GGGAC 4 cut(s) 938, 1825, 2237, 3063
BsmBI CGTCTC 2 cut(s) 1695, 2834
BsmFI GGGAC 4 cut(s) 938, 1825, 2237, 3063
BsmI GAATGC 4 cut(s) 27, 649, 1149, 3036
BsnI GGCC 8 cut(s) 354, 1220, 1426, 1512, 1585, 1709, 2408, 2465
Bso31I GGTCTC 3 cut(s) 1723, 2389, 3016
BsoBI CYCGRG 2 cut(s) 3019, 3026
Bsp120I GGGCCC 1 cut(s) 352
Bsp1286I GDGCHC 3 cut(s) 356, 525, 1232
Bsp13I TCCGGA 1 cut(s) 872
Bsp143I GATC 8 cut(s) 475, 562, 636, 923, 1273, 2391, 2800, 3195
Bsp19I CCATGG 3 cut(s) 1188, 1671, 3004
Bsp68I TCGCGA 1 cut(s) 1635
BspACI CCGC 9 cut(s) 68, 606, 700, 1830, 1860, 2100, 2206, 2925, 2938
BspANI GGCC 8 cut(s) 354, 1220, 1426, 1512, 1585, 1709, 2408, 2465
BspCNI CTCAG 7 cut(s) 33, 1420, 1634, 2044, 2056, 2173, 3074
BspEI TCCGGA 1 cut(s) 872
BspFNI CGCG 2 cut(s) 1635, 2779
BspHI TCATGA 2 cut(s) 580, 1201
BspMAI CTGCAG 2 cut(s) 685, 1570
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 4 cut(s) 483, 1281, 2795, 2808
BspT107I GGYRCC 3 cut(s) 602, 1393, 2493
BspTI CTTAAG 2 cut(s) 248, 3191
BspTNI GGTCTC 3 cut(s) 1723, 2389, 3016
BsrDI GCAATG 5 cut(s) 295, 504, 761, 1317, 2086
BsrFI RCCGGY 1 cut(s) 1456
BsrI ACTGG 5 cut(s) 615, 1658, 1754, 1858, 2556
BssAI RCCGGY 1 cut(s) 1456
BssMI GATC 8 cut(s) 475, 562, 636, 923, 1273, 2391, 2800, 3195
BssT1I CCWWGG 7 cut(s) 35, 774, 1188, 1671, 2631, 2963, 3004
Bst2UI CCWGG 7 cut(s) 186, 285, 377, 840, 1244, 1536, 1957
Bst4CI ACNGT 9 cut(s) 449, 1297, 1527, 1839, 2000, 2065, 2380, 2482, 2873
BstAFI CTTAAG 2 cut(s) 248, 3191
BstAPI GCANNNNNTGC 1 cut(s) 1514
BstBAI YACGTR 1 cut(s) 1236
BstC8I GCNNGC 5 cut(s) 1510, 1830, 2211, 2847, 3103
BstDSI CCRYGG 5 cut(s) 394, 1188, 1671, 1710, 3004
BstFNI CGCG 2 cut(s) 1635, 2779
BstH2I RGCGCY 1 cut(s) 1330
BstHHI GCGC 3 cut(s) 366, 1329, 1630
BstKTI GATC 8 cut(s) 478, 565, 639, 926, 1276, 2394, 2803, 3198
BstMBI GATC 8 cut(s) 475, 562, 636, 923, 1273, 2391, 2800, 3195
BstMCI CGRYCG 1 cut(s) 926
BstMWI GCNNNNNNNGC 7 cut(s) 677, 1514, 2684, 2776, 2978, 3042, 3111
BstNI CCWGG 7 cut(s) 186, 285, 377, 840, 1244, 1536, 1957
BstNSI RCATGY 2 cut(s) 142, 1597
BstPAI GACNNNNGTC 1 cut(s) 3028
BstSCI CCNGG 9 cut(s) 184, 283, 375, 838, 1242, 1534, 1696, 1955, 3122
BstSFI CTRYAG 4 cut(s) 420, 681, 1566, 2259
BstSLI GKGCMC 1 cut(s) 356
BstUI CGCG 2 cut(s) 1635, 2779
BstV1I GCAGC 7 cut(s) 335, 595, 664, 667, 778, 2681, 2909
BstV2I GAAGAC 1 cut(s) 1596
BstX2I RGATCY 4 cut(s) 475, 1273, 2800, 3195
BstXI CCANNNNNNTGG 1 cut(s) 2034
BstYI RGATCY 4 cut(s) 475, 1273, 2800, 3195
BsuI GTATCC 2 cut(s) 153, 898
BsuRI GGCC 8 cut(s) 354, 1220, 1426, 1512, 1585, 1709, 2408, 2465
BtgI CCRYGG 5 cut(s) 394, 1188, 1671, 1710, 3004
BtsIMutI CAGTG 7 cut(s) 525, 1835, 2005, 2244, 2385, 2428, 2478
BtuMI TCGCGA 1 cut(s) 1635
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 5 cut(s) 1510, 1830, 2211, 2847, 3103
CaiI CAGNNNCTG 3 cut(s) 332, 1514, 3255
CciI TCATGA 2 cut(s) 580, 1201
CfoI GCGC 3 cut(s) 366, 1329, 1630
Cfr10I RCCGGY 1 cut(s) 1456
Cfr13I GGNCC 8 cut(s) 352, 353, 1511, 1583, 1707, 1777, 2156, 2464
CseI GACGC 3 cut(s) 600, 2448, 2851
Csp6I GTAC 9 cut(s) 126, 445, 984, 1293, 1571, 2266, 2669, 2912, 3231
CspAI ACCGGT 1 cut(s) 1456
CviQI GTAC 9 cut(s) 126, 445, 984, 1293, 1571, 2266, 2669, 2912, 3231
DpnI GATC 8 cut(s) 477, 564, 638, 925, 1275, 2393, 2802, 3197
DpnII GATC 8 cut(s) 475, 562, 636, 923, 1273, 2391, 2800, 3195
DraI TTTAAA 1 cut(s) 2571
EaeI YGGCCR 1 cut(s) 2406
Eco130I CCWWGG 7 cut(s) 35, 774, 1188, 1671, 2631, 2963, 3004
Eco24I GRGCYC 2 cut(s) 356, 1232
Eco31I GGTCTC 3 cut(s) 1723, 2389, 3016
Eco47I GGWCC 2 cut(s) 1777, 2156
Eco57I CTGAAG 3 cut(s) 1595, 1608, 2609
Eco88I CYCGRG 2 cut(s) 3019, 3026
EcoO109I RGGNCCY 3 cut(s) 352, 1511, 1583
EcoRI GAATTC 2 cut(s) 1444, 2583
EcoRII CCWGG 7 cut(s) 184, 283, 375, 838, 1242, 1534, 1955
EcoT14I CCWWGG 7 cut(s) 35, 774, 1188, 1671, 2631, 2963, 3004
EcoT38I GRGCYC 2 cut(s) 356, 1232
ErhI CCWWGG 7 cut(s) 35, 774, 1188, 1671, 2631, 2963, 3004
Esp3I CGTCTC 2 cut(s) 1695, 2834
FalI AAGNNNNNCTT 6 cut(s) 218, 250, 1355, 1387, 2947, 2979
FaqI GGGAC 4 cut(s) 938, 1825, 2237, 3063
FauI CCCGC 1 cut(s) 1837
FblI GTMKAC 1 cut(s) 423
FriOI GRGCYC 2 cut(s) 356, 1232
FspBI CTAG 7 cut(s) 438, 458, 560, 672, 1787, 2300, 3128
GlaI GCGC 3 cut(s) 365, 1328, 1629
GsaI CCCAGC 1 cut(s) 1583
GsuI CTGGAG 3 cut(s) 1425, 1641, 2106
HaeII RGCGCY 1 cut(s) 1330
HaeIII GGCC 8 cut(s) 354, 1220, 1426, 1512, 1585, 1709, 2408, 2465
HapII CCGG 7 cut(s) 873, 897, 1457, 1505, 1698, 2651, 3123
HgaI GACGC 3 cut(s) 600, 2448, 2851
HhaI GCGC 3 cut(s) 366, 1329, 1630
Hin6I GCGC 3 cut(s) 364, 1327, 1628
HinP1I GCGC 3 cut(s) 364, 1327, 1628
HincII GTYRAC 4 cut(s) 1084, 2080, 2351, 2430
HindII GTYRAC 4 cut(s) 1084, 2080, 2351, 2430
HindIII AAGCTT 4 cut(s) 381, 2371, 2499, 2956
HpaI GTTAAC 1 cut(s) 2430
HpaII CCGG 7 cut(s) 873, 897, 1457, 1505, 1698, 2651, 3123
HphI GGTGA 4 cut(s) 497, 741, 2016, 3217
Hpy99I CGWCG 1 cut(s) 2464
HpyCH4III ACNGT 9 cut(s) 449, 1297, 1527, 1839, 2000, 2065, 2380, 2482, 2873
HpyCH4IV ACGT 3 cut(s) 999, 1235, 1980
HpyF10VI GCNNNNNNNGC 7 cut(s) 677, 1514, 2684, 2776, 2978, 3042, 3111
HpySE526I ACGT 3 cut(s) 999, 1235, 1980
HspAI GCGC 3 cut(s) 364, 1327, 1628
Kpn2I TCCGGA 1 cut(s) 872
KspAI GTTAAC 1 cut(s) 2430
Kzo9I GATC 8 cut(s) 475, 562, 636, 923, 1273, 2391, 2800, 3195
LmnI GCTCC 4 cut(s) 520, 1561, 2125, 3142
Lsp1109I GCAGC 7 cut(s) 335, 595, 664, 667, 778, 2681, 2909
LweI GCATC 4 cut(s) 1249, 1847, 2735, 2896
MaeI CTAG 7 cut(s) 438, 458, 560, 672, 1787, 2300, 3128
MaeII ACGT 3 cut(s) 999, 1235, 1980
MalI GATC 8 cut(s) 477, 564, 638, 925, 1275, 2393, 2802, 3197
MboI GATC 8 cut(s) 475, 562, 636, 923, 1273, 2391, 2800, 3195
MboII GAAGA 9 cut(s) 201, 238, 1601, 2544, 2554, 2848, 2992, 3086, 3109
MfeI CAATTG 1 cut(s) 1043
MflI RGATCY 4 cut(s) 475, 1273, 2800, 3195
MhlI GDGCHC 3 cut(s) 356, 525, 1232
MlsI TGGCCA 1 cut(s) 2408
MluNI TGGCCA 1 cut(s) 2408
MlyI GAGTC 8 cut(s) 425, 944, 1079, 1407, 1549, 2969, 3038, 3181
MmeI TCCRAC 3 cut(s) 200, 1314, 1509
Mox20I TGGCCA 1 cut(s) 2408
MroI TCCGGA 1 cut(s) 872
MroXI GAANNNNTTC 1 cut(s) 2515
MscI TGGCCA 1 cut(s) 2408
MseI TTAA 9 cut(s) 237, 249, 1136, 1692, 1866, 2429, 2484, 2570, 3192
MslI CAYNNNNRTG 1 cut(s) 2423
Msp20I TGGCCA 1 cut(s) 2408
MspA1I CMGCKG 3 cut(s) 608, 710, 2938
MspCI CTTAAG 2 cut(s) 248, 3191
MspI CCGG 7 cut(s) 873, 897, 1457, 1505, 1698, 2651, 3123
MspR9I CCNGG 9 cut(s) 186, 285, 377, 840, 1244, 1536, 1698, 1957, 3124
MunI CAATTG 1 cut(s) 1043
Mva1269I GAATGC 4 cut(s) 27, 649, 1149, 3036
MvaI CCWGG 7 cut(s) 186, 285, 377, 840, 1244, 1536, 1957
MvnI CGCG 2 cut(s) 1635, 2779
MwoI GCNNNNNNNGC 7 cut(s) 677, 1514, 2684, 2776, 2978, 3042, 3111
NciI CCSGG 2 cut(s) 1698, 3124
NcoI CCATGG 3 cut(s) 1188, 1671, 3004
NdeII GATC 8 cut(s) 475, 562, 636, 923, 1273, 2391, 2800, 3195
NmeAIII GCCGAG 2 cut(s) 2791, 2957
NmuCI GTSAC 5 cut(s) 166, 936, 962, 1297, 1381
NruI TCGCGA 1 cut(s) 1635
NspI RCATGY 2 cut(s) 142, 1597
PaeR7I CTCGAG 1 cut(s) 3019
PagI TCATGA 2 cut(s) 580, 1201
PasI CCCWGGG 1 cut(s) 839
PciI ACATGT 1 cut(s) 138
PctI GAATGC 4 cut(s) 27, 649, 1149, 3036
PdmI GAANNNNTTC 1 cut(s) 2515
PflMI CCANNNNNTGG 2 cut(s) 1749, 2503
PfoI TCCNGGA 3 cut(s) 184, 1242, 1696
PinAI ACCGGT 1 cut(s) 1456
Ple19I CGATCG 1 cut(s) 926
PleI GAGTC 8 cut(s) 424, 943, 1079, 1407, 1548, 2969, 3037, 3181
PpsI GAGTC 8 cut(s) 424, 943, 1079, 1407, 1548, 2969, 3037, 3181
Ppu21I YACGTR 1 cut(s) 1236
PscI ACATGT 1 cut(s) 138
PshAI GACNNNNGTC 1 cut(s) 3028
PsiI TTATAA 1 cut(s) 2318
Psp6I CCWGG 7 cut(s) 184, 283, 375, 838, 1242, 1534, 1955
PspFI CCCAGC 1 cut(s) 1579
PspGI CCWGG 7 cut(s) 184, 283, 375, 838, 1242, 1534, 1955
PspOMI GGGCCC 1 cut(s) 352
PspPI GGNCC 8 cut(s) 352, 353, 1511, 1583, 1707, 1777, 2156, 2464
PstI CTGCAG 2 cut(s) 685, 1570
PstNI CAGNNNCTG 3 cut(s) 332, 1514, 3255
PsuI RGATCY 4 cut(s) 475, 1273, 2800, 3195
PvuI CGATCG 1 cut(s) 926
PvuII CAGCTG 1 cut(s) 710
RruI TCGCGA 1 cut(s) 1635
RsaI GTAC 9 cut(s) 127, 446, 985, 1294, 1572, 2267, 2670, 2913, 3232
RsaNI GTAC 9 cut(s) 126, 445, 984, 1293, 1571, 2266, 2669, 2912, 3231
RseI CAYNNNNRTG 1 cut(s) 2423
SaqAI TTAA 9 cut(s) 237, 249, 1136, 1692, 1866, 2429, 2484, 2570, 3192
Sau3AI GATC 8 cut(s) 475, 562, 636, 923, 1273, 2391, 2800, 3195
Sau96I GGNCC 8 cut(s) 352, 353, 1511, 1583, 1707, 1777, 2156, 2464
ScaI AGTACT 2 cut(s) 1294, 1572
SchI GAGTC 8 cut(s) 425, 944, 1079, 1407, 1549, 2969, 3038, 3181
ScrFI CCNGG 9 cut(s) 186, 285, 377, 840, 1244, 1536, 1698, 1957, 3124
SduI GDGCHC 3 cut(s) 356, 525, 1232
SfaNI GCATC 4 cut(s) 1249, 1847, 2735, 2896
SfcI CTRYAG 4 cut(s) 420, 681, 1566, 2259
Sfr274I CTCGAG 1 cut(s) 3019
SinI GGWCC 2 cut(s) 1777, 2156
SlaI CTCGAG 1 cut(s) 3019
SmiMI CAYNNNNRTG 1 cut(s) 2423
SmlI CTYRAG 7 cut(s) 248, 515, 733, 792, 2788, 3019, 3191
SmoI CTYRAG 7 cut(s) 248, 515, 733, 792, 2788, 3019, 3191
SsiI CCGC 9 cut(s) 68, 606, 700, 1830, 1860, 2100, 2206, 2925, 2938
SspI AATATT 2 cut(s) 1764, 2883
SspMI CTAG 7 cut(s) 438, 458, 560, 672, 1787, 2300, 3128
StyD4I CCNGG 9 cut(s) 184, 283, 375, 838, 1242, 1534, 1696, 1955, 3122
StyI CCWWGG 7 cut(s) 35, 774, 1188, 1671, 2631, 2963, 3004
TaaI ACNGT 9 cut(s) 449, 1297, 1527, 1839, 2000, 2065, 2380, 2482, 2873
TaiI ACGT 3 cut(s) 1002, 1238, 1983
TaqI TCGA 9 cut(s) 31, 802, 922, 926, 1088, 1266, 1442, 2748, 3020
TatI WGTACW 7 cut(s) 125, 444, 983, 1292, 1570, 2265, 2668
TauI GCSGC 4 cut(s) 71, 608, 2209, 2927
Tru1I TTAA 9 cut(s) 237, 249, 1136, 1692, 1866, 2429, 2484, 2570, 3192
Tru9I TTAA 9 cut(s) 237, 249, 1136, 1692, 1866, 2429, 2484, 2570, 3192
TscAI CASTG 7 cut(s) 532, 1842, 2005, 2244, 2385, 2428, 2485
TseFI GTSAC 5 cut(s) 166, 936, 962, 1297, 1381
TseI GCWGC 7 cut(s) 323, 608, 677, 680, 766, 2694, 2897
Tsp45I GTSAC 5 cut(s) 166, 936, 962, 1297, 1381
TspGWI ACGGA 5 cut(s) 185, 383, 428, 3065, 3218
TspRI CASTG 7 cut(s) 532, 1842, 2005, 2244, 2385, 2428, 2485
Van91I CCANNNNNTGG 2 cut(s) 1749, 2503
Vha464I CTTAAG 2 cut(s) 248, 3191
VpaK11BI GGWCC 2 cut(s) 1777, 2156
XapI RAATTY 4 cut(s) 1444, 1450, 2444, 2583
XbaI TCTAGA 1 cut(s) 559
XceI RCATGY 2 cut(s) 142, 1597
XhoI CTCGAG 1 cut(s) 3019
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 2515
XspI CTAG 7 cut(s) 438, 458, 560, 672, 1787, 2300, 3128
ZrmI AGTACT 2 cut(s) 1294, 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.