Rroxscaffold_2G00078440

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005447
Physical Location & Seq
Forward (+)
1739956 .. 1748667
8712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078440.1

Sequence Viewer

Length: 3348 bp
ATGGAGGTGCTAGGGTTGAAAGCGAAGTCCAAAATAGCACTTCTCGTTGTTCTCGTGCTGAGCTTTGGCTTCGCCGCGCTCTATGGCCTGTTGAAGCCGGTTTCGAACGGCTGTAACATGACGTATATGTATCCGACGTATATTCCGATACAGATGACTAACTCCGGTGCTGCACCGGCGGCGAAGTATGGGCTGTATTTGTACCACGAAGGCTGGAAGACGATTGATTTCAAGGAGCATTTACAGAAGCTAAGCGGCATTCCGGTGCTTTTTATTCCGGGGAATGGCGGGAGCTATAAGCAGGTGAGGTCATTGGCTGCGGAATCTGATAGGGCGTATAAAGTAGGTCCACTTGAGCGTACGTTTTATGAAGAAGCTCGGTTGACTCCGGAAGAGGGAGGGGAGGAGGATATGGATGCAGCTGGCTCTGAGTTGAAGAACCAATACGATTCGAGGTTGGACTGGTTCGCTGTGGATCTTGAAGGAGAACACTCTGCTATGGATAGTGCTGTACTTGAACAACACACACAATATGTTGTCCGCTGCATTCACAGGATTTTGGATCAGTACAAGGAGTCTTTCAAGGCTAGAGAAAAGGAAGGAGCTGCAACCTCTGCGACTTTGCCAAAAAGTGTTATATTGGTTGGTCACTCTATGGGTGGTTTCGTTGCTAGAGCTGCAGTGGTCGACCCTCTTTTGAGAAAGTCAGCAGTTGAAACAATTATTACACTTTCCAGCCCACACCAACAACCTCCTGTGGCACTGCAGCCTTCCTACGGTCACTACTTTAGACATGTTAATCGAGAATGGAGAAAGGGATATGAGGTCCAAACAACTGCAGCAGGACATTACTTGTCTGGTCCAGCTCTCTCTAATGTTGTTGTCATATCTATTTCTGGTGGTTATAATGATTACCAGGTAAGGACCAAGTATGAATCCCTTGATGGCATTGTGCCGCCTACCCATGGTTTTATGATCAGTAGTACCGGCATGAAAAATGTTTGGTTATCCATGGAACATCAAGTTATCTTATGGTGCAATCAATTAGTTATCCAAGTATCACACACTCTCCTTAGTTTGGCAGATTCCAGAACAGGCCAGCCTTTCTCTGACACACGAATAAGACTTGCAATATTCTCGAGAATGCTTCGTAGTGCAATACCACAAAGTTTCAATTGGAGGAAGCAATCGCAATTGTCTCAACAGTCAATGCATGCTCCAACTAGAGACGTGAAAGACAGAACTGAATCACTATACATCCCTGCTGCTTGTCCTAGCAATGTTCATTGGAGTGATGATGGCCTTGAGAGGGACTTATACATTCAGACAACCACTGTCACTGTTTTAGCCATGGATGGTCGAAGACGGTGGTTGGACATTCAAAAACTGGGATCCAATGGAAGAAGTCACTTCATGTTTGTGACCAACCTTGCTCCATGTTCTGGGGTTAGACTTCATCTATGGCCTGACAAGAGAAACTCAACTTCAGACTTGCCTGCATGTATAAGGATCCATGAGGTGACATCGAAGATGGTGCTCATTCCATCAGGACCAGCACCAAGACAGATTGAACCAGGAAGCCAAACAGAGCAAGCTCCCCCTTCAGCTATACTCCGCTTAGGACCCAAGGATATGCGTGGTTTCAGATTCCTGACCATCTCAGTTGCACCACGTCCGAGTATTTCAGGTAGACCTCCACCAGCAGTTTCCATGGCAGTTGGTCAGTTTTTTAATCCAGCGGAAGGGGAGAGAGAGATCTCTCCTTGGTCACCTCCACTGTCTGGTTATTCTCACAAGGATTTATCTTGGAAGGAAGATCATCCCCTTGCTTTGAATCTATCATTCACAAGTAGTTTAGGCCTTCTGCCTGTAAAGTTCTCTTTGAAGACCGCAGGTTGTGGAATAAAGAGTTCCGGACTTCCAGATGAACAGGCTGGAGATATCGATAATAGCAAGCTCTGTAAGCTTCGCTGTTTCCCGCCAGTTGCATTTGCTTGGGATGAAACAGCAGGTCTCCACGTATTCACAGATTTGTATAGTGACAAAATTGTTGTTGATTCATCACCATTGCTTTGGAGTTCACCTAGAAGTTCTGAGATAACCTCTGTTATGTTGGTGGTAGATCCACATTGTTCGTATAGCAGTGCGATGACTGTTTCTGTAACTGCAGTTGCTAGTAGGTTTTTGCTTGTGTATACTTCACAGATTATTGGTTTTTCCATTGTTGTTATCTTTTTTGCACTGATGCGGCAAGCATGTGCATGGGATCGTAACCTACCCATACCCTCGATGTTGAAGGCTCTGGAGTCCAATTTGAGATTCCCAATTCCATTTATCTACCTTGCTATTGTACCAATCGGTCTTTCTTTACTCGTTGGTTTTTGGATTTCTCAACCATCTCCTTCATTTGCTAGCTTCACCATTGTATCAGTTGTCTGCTATTTCCTTGCTAATGGCTTCATAGCCATTCTGATTTTGACATCCCAGTTCGCCTTTTATGGGGCTGCTCTGCTACATATTTTCATCAAGAAAAGGTTTCAATTGGGGGGAAAAACTTCTCAATTGTTTGTGAATATATCCTCTGGTTTCTGTTCATGGAAGGTGGTAAGGGTTATAAGAGCTAATCCACTAATGGTTACAGCACTTGCTGCAATTACTTTGGCATGCTTGGTTCATCCGGGTTTGGGTCTACTTGTTCTCGTGCTCTCTCATGCTTTGTGCTGTCATACTGCTCTTTGCAGTTTTTTGACAGCTTCATTTCGCAGCCATGCACGAAGACATGAATTATTTGATTATAAGAAAGACGGAAATGGTGGCCCTGACAAACTTACATTCAAACGTGATGGTATATCAAACCAGAATTTTTCTTCTGAAGATACGTGCTCCAATAGCCCAGACTCTTCAAAAAGTTTTGGTGAAACTCAATTAGAGATATTCCATCATCGGCATGGGTTGTTTATTTTGCATCTTGTTGCAACACTCATGTTTGGCCCATCATTTGTGACTTGGTTGGAGAGAATTGGGATGGATCATAGCTTTCCATGGGTCTTGGATTCAGCTCTTTCCACCGGTGTGATCCTTCATGGTATCTTCACTTCAAACCCTCAGTTCAATTCCTTCCTGGTTTCTTTCCCTCCATTCCGAAACCTAGAAGTGAGGATGCATTTACTGTACCTGTTTGCTGGATACTATTCTTATCTCTCTTCCCTGGCCTTGGCTCCATATAGAGAATTTTATGTTATGGCTGCTGTAGGCTATACTTCAATTGGTCTGACCCTCTTACAGAGATGGAACAAGGAGAAGGGAGATGCCCATTTTGTTAACCGAAAGCACTCTCACAGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

1115

Amino Acids

123.85

Weight (kDa)

8.94

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGAP1 PF07819 84 - 359 2.4e-82 PGAP1-like alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 906, 2615, 2796
AarI CACCTGC 1 cut(s) 292
Acc36I ACCTGC 3 cut(s) 292, 1883, 2000
AccB7I CCANNNNNTGG 2 cut(s) 1442, 1781
AccI GTMKAC 4 cut(s) 687, 1690, 2195, 2689
AccII CGCG 1 cut(s) 77
AccIII TCCGGA 2 cut(s) 388, 1913
AcsI RAATTY 2 cut(s) 2860, 3230
AcuI CTGAAG 3 cut(s) 1470, 1587, 2892
AfaI GTAC 7 cut(s) 203, 361, 513, 569, 985, 2352, 3173
AflIII ACRYGT 1 cut(s) 793
AgeI ACCGGT 1 cut(s) 3068
AjiI CACGTC 2 cut(s) 1231, 1673
AjnI CCWGG 4 cut(s) 915, 1573, 3120, 3207
AjuI GAANNNNNNNTTGG 4 cut(s) 2303, 2335, 2347, 2379
AleI CACNNNNGTG 1 cut(s) 3071
Alw21I GWGCWC 3 cut(s) 1539, 2706, 2885
Alw26I GTCTC 3 cut(s) 1203, 1221, 2018
AlwNI CAGNNNCTG 1 cut(s) 3345
Ama87I CYCGRG 1 cut(s) 1138
Aor13HI TCCGGA 2 cut(s) 388, 1913
AoxI GGCC 8 cut(s) 85, 1096, 1300, 1463, 1858, 2815, 2989, 3210
ApoI RAATTY 2 cut(s) 2860, 3230
AsiGI ACCGGT 1 cut(s) 3068
Asp700I GAANNNNTTC 1 cut(s) 2554
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
AsuC2I CCSGG 2 cut(s) 279, 2679
AsuHPI GGTGA 7 cut(s) 316, 1531, 1761, 2055, 2073, 2410, 2927
AsuII TTCGAA 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 2411
AvaI CYCGRG 1 cut(s) 1138
AvaII GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
BaeI ACNNNNGTAYC 2 cut(s) 2409, 2442
BamHI GGATCC 2 cut(s) 1391, 1509
BarI GAAGNNNNNNTAC 4 cut(s) 428, 460, 3079, 3111
BauI CACGAG 2 cut(s) 53, 2699
BbsI GAAGAC 4 cut(s) 224, 1369, 1892, 2782
Bbv12I GWGCWC 3 cut(s) 1539, 2706, 2885
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 4 cut(s) 1119, 1153, 1516, 1550
BciT130I CCWGG 4 cut(s) 917, 1575, 3122, 3209
BciVI GTATCC 2 cut(s) 141, 3179
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 2 cut(s) 279, 2679
BcoDI GTCTC 3 cut(s) 1203, 1221, 2018
BfaI CTAG 9 cut(s) 11, 588, 672, 1224, 1275, 2085, 2175, 2412, 3149
BfmI CTRYAG 5 cut(s) 678, 764, 837, 2166, 3249
BfuAI ACCTGC 3 cut(s) 292, 1883, 2000
BfuI GTATCC 2 cut(s) 141, 3179
BglII AGATCT 1 cut(s) 1755
BlpI GCTNAGC 2 cut(s) 59, 251
Bme1390I CCNGG 6 cut(s) 279, 917, 1575, 2679, 3122, 3209
Bme18I GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
BmeT110I CYCGRG 1 cut(s) 1138
BmgBI CACGTC 2 cut(s) 1231, 1673
BmgT120I GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
BmiI GGNNCC 4 cut(s) 1393, 1511, 1624, 3219
BmrFI CCNGG 6 cut(s) 279, 917, 1575, 2679, 3122, 3209
BmrI ACTGGG 2 cut(s) 1397, 2479
BmsI GCATC 5 cut(s) 406, 2235, 2974, 3150, 3298
BmtI GCTAGC 1 cut(s) 2415
BmuI ACTGGG 2 cut(s) 1397, 2479
BpiI GAAGAC 4 cut(s) 224, 1369, 1892, 2782
BplI GAGNNNNNCTC 2 cut(s) 2087, 2119
BpmI CTGGAG 2 cut(s) 1956, 2324
Bpu10I CCTNAGC 1 cut(s) 1618
Bpu1102I GCTNAGC 2 cut(s) 59, 251
Bpu14I TTCGAA 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 374, 1325
BpuMI CCSGG 2 cut(s) 279, 2679
Bsa29I ATCGAT 1 cut(s) 1944
BsaAI YACGTR 2 cut(s) 2020, 2880
BsaI GGTCTC 1 cut(s) 2018
BsaWI WCCGGW 5 cut(s) 164, 262, 388, 1913, 3068
BsaXI ACNNNNNCTCC 2 cut(s) 1053, 1083
Bse118I RCCGGY 4 cut(s) 97, 175, 986, 3068
Bse1I ACTGG 4 cut(s) 467, 1392, 1982, 2485
Bse3DI GCAATG 2 cut(s) 1285, 2066
BseAI TCCGGA 2 cut(s) 388, 1913
BseBI CCWGG 4 cut(s) 917, 1575, 3122, 3209
BseCI ATCGAT 1 cut(s) 1944
BseGI GGATG 9 cut(s) 421, 1257, 1360, 1819, 2005, 2480, 2674, 3030, 3165
BseMI GCAATG 2 cut(s) 1285, 2066
BseMII CTCAG 5 cut(s) 50, 420, 1674, 2085, 3119
BseNI ACTGG 4 cut(s) 467, 1392, 1982, 2485
BseRI GAGGAG 1 cut(s) 419
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 8 cut(s) 87, 1098, 1302, 1465, 1860, 2817, 2991, 3212
BshTI ACCGGT 1 cut(s) 3068
BshVI ATCGAT 1 cut(s) 1944
BsiHKAI GWGCWC 3 cut(s) 1539, 2706, 2885
BsiHKCI CYCGRG 1 cut(s) 1138
BsiWI CGTACG 1 cut(s) 359
BslFI GGGAC 1 cut(s) 1325
BsmAI GTCTC 3 cut(s) 1203, 1221, 2018
BsmBI CGTCTC 1 cut(s) 1221
BsmFI GGGAC 1 cut(s) 1325
BsmI GAATGC 3 cut(s) 258, 546, 1149
BsnI GGCC 8 cut(s) 87, 1098, 1302, 1465, 1860, 2817, 2991, 3212
Bso31I GGTCTC 1 cut(s) 2018
BsoBI CYCGRG 1 cut(s) 1138
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 3 cut(s) 1539, 2706, 2885
Bsp13I TCCGGA 2 cut(s) 388, 1913
Bsp1720I GCTNAGC 2 cut(s) 59, 251
Bsp19I CCATGG 5 cut(s) 964, 1011, 1350, 1710, 3041
BspANI GGCC 8 cut(s) 87, 1098, 1302, 1465, 1860, 2817, 2991, 3212
BspCNI CTCAG 5 cut(s) 51, 421, 1673, 2086, 3118
BspDI ATCGAT 1 cut(s) 1944
BspEI TCCGGA 2 cut(s) 388, 1913
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 4 cut(s) 1393, 1511, 1624, 3219
BspMAI CTGCAG 4 cut(s) 682, 768, 841, 2170
BspMI ACCTGC 3 cut(s) 292, 1883, 2000
BspOI GCTAGC 1 cut(s) 2415
BspT104I TTCGAA 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 2018
BsrDI GCAATG 2 cut(s) 1285, 2066
BsrFI RCCGGY 4 cut(s) 97, 175, 986, 3068
BsrI ACTGG 4 cut(s) 467, 1392, 1982, 2485
BssAI RCCGGY 4 cut(s) 97, 175, 986, 3068
BssNAI GTATAC 1 cut(s) 2196
BssSI CACGAG 2 cut(s) 53, 2699
BssT1I CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Bst1107I GTATAC 1 cut(s) 2196
Bst2BI CACGAG 2 cut(s) 53, 2699
Bst2UI CCWGG 4 cut(s) 917, 1575, 3122, 3209
Bst4CI ACNGT 8 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155, 3171
Bst6I CTCTTC 3 cut(s) 387, 2905, 3208
BstAPI GCANNNNNTGC 2 cut(s) 614, 2648
BstBAI YACGTR 2 cut(s) 2020, 2880
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 9 cut(s) 424, 1100, 1215, 1497, 1593, 1955, 2253, 2413, 2665
BstDEI CTNAG 8 cut(s) 59, 251, 429, 1073, 1618, 1660, 2094, 3105
BstDSI CCRYGG 5 cut(s) 964, 1011, 1350, 1710, 3041
BstEII GGTNACC 1 cut(s) 1767
BstF5I GGATG 9 cut(s) 421, 1257, 1360, 1819, 2005, 2480, 2674, 3030, 3165
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 1 cut(s) 79
BstMAI GTCTC 3 cut(s) 1203, 1221, 2018
BstMWI GCNNNNNNNGC 7 cut(s) 176, 179, 614, 677, 1963, 2648, 2889
BstNI CCWGG 4 cut(s) 917, 1575, 3122, 3209
BstNSI RCATGY 5 cut(s) 797, 1217, 1503, 2259, 2667
BstPI GGTNACC 1 cut(s) 1767
BstSCI CCNGG 6 cut(s) 277, 915, 1573, 2677, 3120, 3207
BstSFI CTRYAG 5 cut(s) 678, 764, 837, 2166, 3249
BstUI CGCG 1 cut(s) 77
BstV2I GAAGAC 4 cut(s) 224, 1369, 1892, 2782
BstX2I RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
BstXI CCANNNNNNTGG 1 cut(s) 2073
BstYI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
BstZ17I GTATAC 1 cut(s) 2196
Bsu15I ATCGAT 1 cut(s) 1944
BsuI GTATCC 2 cut(s) 141, 3179
BsuRI GGCC 8 cut(s) 87, 1098, 1302, 1465, 1860, 2817, 2991, 3212
BsuTUI ATCGAT 1 cut(s) 1944
BtgI CCRYGG 5 cut(s) 964, 1011, 1350, 1710, 3041
BtgZI GCGATG 1 cut(s) 2162
BtrI CACGTC 2 cut(s) 1231, 1673
BtsCI GGATG 9 cut(s) 421, 1257, 1360, 1819, 2005, 2480, 2674, 3030, 3165
BtsI GCAGTG 3 cut(s) 687, 761, 2149
BtsIMutI CAGTG 8 cut(s) 687, 761, 1332, 1338, 1775, 2149, 2240, 3343
BveI ACCTGC 3 cut(s) 292, 1883, 2000
Cac8I GCNNGC 9 cut(s) 424, 1100, 1215, 1497, 1593, 1955, 2253, 2413, 2665
CaiI CAGNNNCTG 1 cut(s) 3345
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 4 cut(s) 97, 175, 986, 3068
Cfr13I GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
ClaI ATCGAT 1 cut(s) 1944
CsiI ACCWGGT 1 cut(s) 915
Csp6I GTAC 7 cut(s) 202, 360, 512, 568, 984, 2351, 3172
CspAI ACCGGT 1 cut(s) 3068
CviQI GTAC 7 cut(s) 202, 360, 512, 568, 984, 2351, 3172
DdeI CTNAG 8 cut(s) 59, 251, 429, 1073, 1618, 1660, 2094, 3105
Eam1104I CTCTTC 3 cut(s) 387, 2905, 3208
EarI CTCTTC 3 cut(s) 387, 2905, 3208
Eco130I CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Eco147I AGGCCT 1 cut(s) 1860
Eco31I GGTCTC 1 cut(s) 2018
Eco32I GATATC 1 cut(s) 1942
Eco47I GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
Eco57I CTGAAG 3 cut(s) 1470, 1587, 2892
Eco88I CYCGRG 1 cut(s) 1138
Eco91I GGTNACC 1 cut(s) 1767
EcoO109I RGGNCCY 1 cut(s) 1622
EcoO65I GGTNACC 1 cut(s) 1767
EcoRII CCWGG 4 cut(s) 915, 1573, 3120, 3207
EcoRV GATATC 1 cut(s) 1942
EcoT14I CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
EcoT22I ATGCAT 2 cut(s) 1215, 3165
ErhI CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Esp3I CGTCTC 1 cut(s) 1221
FaqI GGGAC 1 cut(s) 1325
FauI CCCGC 2 cut(s) 281, 1986
FbaI TGATCA 1 cut(s) 975
FblI GTMKAC 4 cut(s) 687, 1690, 2195, 2689
FokI GGATG 9 cut(s) 428, 1244, 1367, 1806, 2012, 2467, 2661, 3037, 3172
FspBI CTAG 9 cut(s) 11, 588, 672, 1224, 1275, 2085, 2175, 2412, 3149
GlaI GCGC 1 cut(s) 78
GsuI CTGGAG 2 cut(s) 1956, 2324
HaeIII GGCC 8 cut(s) 87, 1098, 1302, 1465, 1860, 2817, 2991, 3212
HhaI GCGC 1 cut(s) 79
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 3 cut(s) 384, 688, 3322
HindII GTYRAC 3 cut(s) 384, 688, 3322
HindIII AAGCTT 1 cut(s) 1964
HpaI GTTAAC 1 cut(s) 3322
HphI GGTGA 7 cut(s) 316, 1531, 1761, 2055, 2073, 2410, 2927
Hpy166II GTNNAC 8 cut(s) 350, 384, 688, 1691, 2081, 2196, 2690, 3322
Hpy8I GTNNAC 8 cut(s) 350, 384, 688, 1691, 2081, 2196, 2690, 3322
Hpy99I CGWCG 1 cut(s) 139
HpyCH4III ACNGT 8 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155, 3171
HpyCH4IV ACGT 8 cut(s) 122, 137, 362, 1230, 1672, 2019, 2839, 2879
HpyF10VI GCNNNNNNNGC 7 cut(s) 176, 179, 614, 677, 1963, 2648, 2889
HpyF3I CTNAG 8 cut(s) 59, 251, 429, 1073, 1618, 1660, 2094, 3105
HpySE526I ACGT 8 cut(s) 122, 137, 362, 1230, 1672, 2019, 2839, 2879
HspAI GCGC 1 cut(s) 77
Kpn2I TCCGGA 2 cut(s) 388, 1913
Ksp22I TGATCA 1 cut(s) 975
KspAI GTTAAC 1 cut(s) 3322
LmnI GCTCC 8 cut(s) 235, 291, 602, 1222, 1438, 1600, 2888, 3223
LweI GCATC 5 cut(s) 406, 2235, 2974, 3150, 3298
MabI ACCWGGT 1 cut(s) 915
MaeI CTAG 9 cut(s) 11, 588, 672, 1224, 1275, 2085, 2175, 2412, 3149
MaeII ACGT 8 cut(s) 122, 137, 362, 1230, 1672, 2019, 2839, 2879
MfeI CAATTG 5 cut(s) 1174, 1193, 2540, 2561, 3264
MflI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
MhlI GDGCHC 3 cut(s) 1539, 2706, 2885
MlyI GAGTC 4 cut(s) 379, 584, 2315, 2891
MmeI TCCRAC 5 cut(s) 158, 438, 1244, 1353, 2991
Mph1103I ATGCAT 2 cut(s) 1215, 3165
MroI TCCGGA 2 cut(s) 388, 1913
MroXI GAANNNNTTC 1 cut(s) 2554
MseI TTAA 3 cut(s) 798, 1731, 3321
MslI CAYNNNNRTG 6 cut(s) 263, 1290, 1418, 2260, 2946, 3071
MspA1I CMGCKG 3 cut(s) 422, 543, 1739
MspR9I CCNGG 6 cut(s) 279, 917, 1575, 2679, 3122, 3209
MunI CAATTG 5 cut(s) 1174, 1193, 2540, 2561, 3264
Mva1269I GAATGC 3 cut(s) 258, 546, 1149
MvaI CCWGG 4 cut(s) 917, 1575, 3122, 3209
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 7 cut(s) 176, 179, 614, 677, 1963, 2648, 2889
NciI CCSGG 2 cut(s) 279, 2679
NcoI CCATGG 5 cut(s) 964, 1011, 1350, 1710, 3041
NheI GCTAGC 1 cut(s) 2411
NlaIV GGNNCC 4 cut(s) 1393, 1511, 1624, 3219
NmuCI GTSAC 9 cut(s) 647, 779, 1336, 1406, 1420, 1519, 1767, 2039, 3001
NsiI ATGCAT 2 cut(s) 1215, 3165
NspI RCATGY 5 cut(s) 797, 1217, 1503, 2259, 2667
NspV TTCGAA 1 cut(s) 104
OliI CACNNNNGTG 1 cut(s) 3071
PaeI GCATGC 2 cut(s) 1217, 2667
PaeR7I CTCGAG 1 cut(s) 1138
PaqCI CACCTGC 1 cut(s) 292
PceI AGGCCT 1 cut(s) 1860
PciI ACATGT 1 cut(s) 793
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 143
PctI GAATGC 3 cut(s) 258, 546, 1149
PdmI GAANNNNTTC 1 cut(s) 2554
Pfl23II CGTACG 1 cut(s) 359
PflMI CCANNNNNTGG 2 cut(s) 1442, 1781
PinAI ACCGGT 1 cut(s) 3068
PleI GAGTC 4 cut(s) 379, 583, 2314, 2891
PpsI GAGTC 4 cut(s) 379, 583, 2314, 2891
Ppu21I YACGTR 2 cut(s) 2020, 2880
PpuMI RGGWCCY 1 cut(s) 1622
PscI ACATGT 1 cut(s) 793
PsiI TTATAA 3 cut(s) 906, 2615, 2796
Psp5II RGGWCCY 1 cut(s) 1622
Psp6I CCWGG 4 cut(s) 915, 1573, 3120, 3207
PspEI GGTNACC 1 cut(s) 1767
PspGI CCWGG 4 cut(s) 915, 1573, 3120, 3207
PspLI CGTACG 1 cut(s) 359
PspN4I GGNNCC 4 cut(s) 1393, 1511, 1624, 3219
PspPI GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
PspPPI RGGWCCY 1 cut(s) 1622
PstI CTGCAG 4 cut(s) 682, 768, 841, 2170
PstNI CAGNNNCTG 1 cut(s) 3345
PsuI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 7 cut(s) 203, 361, 513, 569, 985, 2352, 3173
RsaNI GTAC 7 cut(s) 202, 360, 512, 568, 984, 2351, 3172
RseI CAYNNNNRTG 6 cut(s) 263, 1290, 1418, 2260, 2946, 3071
SalI GTCGAC 1 cut(s) 686
SaqAI TTAA 3 cut(s) 798, 1731, 3321
Sau96I GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
SchI GAGTC 4 cut(s) 379, 584, 2315, 2891
ScrFI CCNGG 6 cut(s) 279, 917, 1575, 2679, 3122, 3209
SduI GDGCHC 3 cut(s) 1539, 2706, 2885
SexAI ACCWGGT 1 cut(s) 915
SfaNI GCATC 5 cut(s) 406, 2235, 2974, 3150, 3298
SfcI CTRYAG 5 cut(s) 678, 764, 837, 2166, 3249
Sfr274I CTCGAG 1 cut(s) 1138
SfuI TTCGAA 1 cut(s) 104
SgrAI CRCCGGYG 2 cut(s) 175, 3068
SinI GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
SlaI CTCGAG 1 cut(s) 1138
SmiMI CAYNNNNRTG 6 cut(s) 263, 1290, 1418, 2260, 2946, 3071
SmlI CTYRAG 3 cut(s) 353, 1138, 1304
SmoI CTYRAG 3 cut(s) 353, 1138, 1304
SphI GCATGC 2 cut(s) 1217, 2667
SseBI AGGCCT 1 cut(s) 1860
SspI AATATT 1 cut(s) 1134
SspMI CTAG 9 cut(s) 11, 588, 672, 1224, 1275, 2085, 2175, 2412, 3149
StuI AGGCCT 1 cut(s) 1860
StyD4I CCNGG 6 cut(s) 277, 915, 1573, 2677, 3120, 3207
StyI CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
TaaI ACNGT 8 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155, 3171
TaiI ACGT 8 cut(s) 125, 140, 365, 1233, 1675, 2022, 2842, 2882
TaqI TCGA 9 cut(s) 104, 452, 687, 802, 1139, 1360, 1526, 1944, 2288
TaqII GACCGA 1 cut(s) 2348
TatI WGTACW 2 cut(s) 511, 567
TauI GCSGC 5 cut(s) 77, 182, 258, 958, 2251
Tru1I TTAA 3 cut(s) 798, 1731, 3321
Tru9I TTAA 3 cut(s) 798, 1731, 3321
TscAI CASTG 7 cut(s) 687, 768, 1339, 1345, 1782, 2149, 2247
TseFI GTSAC 9 cut(s) 647, 779, 1336, 1406, 1420, 1519, 1767, 2039, 3001
Tsp45I GTSAC 9 cut(s) 647, 779, 1336, 1406, 1420, 1519, 1767, 2039, 3001
TspGWI ACGGA 1 cut(s) 2820
TspRI CASTG 7 cut(s) 687, 768, 1339, 1345, 1782, 2149, 2247
Van91I CCANNNNNTGG 2 cut(s) 1442, 1781
VpaK11BI GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
XapI RAATTY 2 cut(s) 2860, 3230
XceI RCATGY 5 cut(s) 797, 1217, 1503, 2259, 2667
XcmI CCANNNNNNNNNTGG 1 cut(s) 2945
XhoI CTCGAG 1 cut(s) 1138
XmiI GTMKAC 4 cut(s) 687, 1690, 2195, 2689
XmnI GAANNNNTTC 1 cut(s) 2554
XspI CTAG 9 cut(s) 11, 588, 672, 1224, 1275, 2085, 2175, 2412, 3149
Zsp2I ATGCAT 2 cut(s) 1215, 3165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.