MD09G1017700.v1.1

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005447
Physical Location & Seq
Forward (+)
1099924 .. 1107138
7215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1017700.v1.1.491

Sequence Viewer

Length: 3339 bp
ATGCAACTGCAAGGATTTAAAGCCCAATCCAGAGTAGCACTGCTCGTCGCCTTCCTCTTGTGTGTTTGTTTCGCGGGTTTATATGATTTGTTGAAGCCCGTCTCCAATGGCTGCATCATGACGTACATGTATCCCACTTATGTTCCCATCCCTACCACGACTCCCGTTTCGCCTGCGAAATACAGCTTGTATTTGTACCACGAAGGATGGAAGAAGATCGATTTTGAGGAGCATCTGAAGAAGCTGAGCGGCGTTCCCGTGCTTTTCATTCCGGGAAATGGCGGCAGCTACAAGCAGGTTCGATCGCTTGCAGCAGAATCTGATAGGGGATATCAAGCGGGGCCCCTAGAGCGTACATATTATCAGGAAGCTTACTTAACTCCTGAAGAGGGAGGGGAGGAGATTGATGTGACTTCCTTTAAGTTGCCCAACCGGTATGATTCCAGGTTGGACTGGTTCACGGTGGATCTCGAAGGGGAACATTCTGCTATGGATAGTGCTATTCTTGAAGAACATGCTGAATATGTTGTCAACTCCATTCACAGGATTTTGGATCAATACAAAGAATCTTATAAGGCTAGACAAAGAGAAGGTGCTGCAACCTCTGGGAGTTCTCCAAAAAGTGTGATATTGGTTGGTCACTCTATGGGTGGTTTTGTTGCTAGAGCTGCAGTTATCCATCACCGTTTGAGGAAATCAGCAGTTGAAACAATTCTTACTCTCTCCAGCCCCCACCAATACCCTCCTGTGGCATTGCAGCCTTCCTTGGGTCACTACTTTGAACGTGTTAATGATGAATGGAGAAAGGGATATGAGGTCCAAACAACTAGAGCAGGACATCACGTGTCTGGTCCTGTTCTCTCCCATGTTGTTGTTATATCCATTTCTGGTGGTTATAATGATTATCAGGTAAGATCCAAGTCGGAATCCCTTGATGGCATTGTGCCTCCCACTCATGGCTTTGTGATCAGCAGTACAGGCATGAGAAATGTGTGGTTGTCAATGGAACATCAAGCTATTTTATGGTGTAATCAATTAGTTATCCAAGTAGCGCACACCCTCCTTAGTTTGGTAGACTCCGGGACAGGCCAGCCATTTTCTGACACTCGAATAAGAACAGCGATATTCTCCAAAATGCTTCGTAGTGGGATACCCCAGAGTTTCGATTGGAGGATGCAATCACACTTATCTCAGCAGTCAATACACATTCCTACTAGAGACGTAAAAGATAAAACTGAATCGCTGTACACTTCCGCTGCATGTCCTAGCAATGTTCATTGGAGTGATGATGGCCTTGAGAGGGACCTATACATTCAGACAACCACCGTCACTGTTTTAGCTATGGATGGTCGAAGACGGTGGTTAGACATACAAAAATTGGGGTCAAATGGAAGAAGCCACTTCATGTTCGTGACAAACCTTGCTCCGTGTTCTGGGGTTAGACTTCATCTGTGGCCAGAAAAAAGAAACTCAACTTCAGAATTGCCTATTTGTATAAGAATCCTCGATGTGACATCAAGGATGGTGCGCATTCCATCAGGACCAGCACCAACACAGATTGAACCTGGAAGCCAGACGGAGCAAGCTTCTCCTTCTGCTATATTTCGCTTGGGACCTGAGGACATGCGTGGTTTCAGATTCCTGACCATCTCAGTTGCACCTCGTCCGACTATTTCAGGCAGACCTCCACCAGCAGTTTCCATGGCTGTCGGGCAGTTTTTTAATCCAGAAGAAGGGGAACGAGAGTTCTCCCCTTGGTCTATGCCGCTACCTAGTTATTCTTATAAGGAAATGTCTTTGAAGGAGGATCATCCTCTTGCTCTGAATCTATCATTTACCACGAGCTTGGGCCTTCTGCCTGTCATGTTCTCTTTGAAGGCTGCAGGCTGTGGGATAATGAATTCTGGACTTCCAGACGAACAGGCTGGAGATGAAGATAATAGCAAGCTCTGTAAGCTTCGCTGTTTCCCTCCTATAGCATTTGCTTGGGATCACACGTCAGGTCTCCACATATTCCCAAATATGTACAGTGAGAAAATTGTTGTTGATTCTTCACCAGCACTATGGAGTTCACCTCAAAGTTCCGAGAAAACCTCTGTTATGTTGCTGGTAGATCCACATTGTTCATATAGAAGTTCCATGACTGTTCCTGTAACTGCAGCTGCCAGTAGGTTCTTGCTTTTATATAATTCACAGATTGCTGGTTTCTCCCTTGTTGTTATCTTTTTTGCGCTAATGCAGCAAATATGTACGTGGGATCTTGACCAACACATACCTTCGATTCTTACGGCTGTGGAATTTAATCTGAGAATCCCATTGCCATTTCTCTACCTTGCCATTGCCCCGATCTTGCTTTCTTTTTCCCTCTCCTTTTTGATTTCTCAACCATTTCCTTCATTTTCTAGCTTCACCATCGTCTCGGTGACTTGTTATTTACTTGCTAATGGCTTCGTAATAATTCTGATTTTGATATCCCAACTCATCTTCTATGCGGCCGCTGTTGTACATGTTTTCATCAAGACAAGGTTTCAGTTGGGGGAAAAAAGTGTTCACCGGTTTATTAATCTTTCTTCGGGTTTCTTTTCATTGAAGCTGGTAAGGGTTTTAAGAGCCAATCCGGTATTCGTTACAGCACTTGTTGCAATTACTGTGGCGTGCTTGGTTCATGCAGCGTTCGGTCTATTTATAATCTTGTTCTTCGATGCTTTGAGCTGTCACAGTGCACTTTGCAGTTTTTTGACAGCTTCATTTCGCAGTCATGCACGAAGACATGAATTATTTGACTGTAAAAAAGAAGACAATGGCCAGTCCTGCCAACTTCCATCCAAAAGTGATGGTATATCAAACCAGAATATTCATTCTGAAGATTGCTGCTCCAACAGTCCAAACTCTTCGAAAAGTTTTGGTGAAACACAGTTAGAGTTATTCCATCATCGGCATGGGCTGTTTATTTTGCATCTTGCTGCAGCGCTCATGTTTGTCCCGTCACTTGTCGCCTGGTTCCAGAGAATAGGGATGGGTCATAGCTTCCCATGGCTCTTGGATTCATTTCTTTGCACAGGTGTGATCCTTCATGGAATCTTCACCTCAAAGCCCGAGTCCAGTTCCTTCTTGGTATCTTTCCCTGGCTTCCGAAACTGTGAAGTGAGGCTGAATTTTCTGTACCTGCTAGCTGGATACTATTCCTATATCTCTTCCCTGGCCTTGGCTCCGTACAGAGCATTTTATGGTATGGCTGCTATAGGCTTTACTTGCTGTGCTTTGACGATCTTACAAAGTCGGAACAGAGAAAAGGGAGAGCCCCATTTTGTTAGCCGAAAGCACTCCCACAGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

1113

Amino Acids

124.03

Weight (kDa)

8.19

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGAP1 PF07819 82 - 356 1.3e-81 PGAP1-like alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 573, 897, 1785, 2687
Acc16I TGCGCA 1 cut(s) 1529
Acc36I ACCTGC 2 cut(s) 286, 3174
AccBSI CCGCTC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 1074
AccII CGCG 1 cut(s) 74
AciI CCGC 8 cut(s) 74, 249, 282, 338, 1254, 1766, 2492, 2496
AclWI GGATC 8 cut(s) 474, 561, 909, 1815, 1998, 2108, 2265, 3061
AcoI YGGCCR 3 cut(s) 1454, 2493, 2803
AcsI RAATTY 3 cut(s) 1900, 2297, 3154
AcuI CTGAAG 4 cut(s) 257, 405, 1461, 2883
AcvI CACGTG 1 cut(s) 844
AfeI AGCGCT 1 cut(s) 2970
AflIII ACRYGT 5 cut(s) 126, 784, 843, 1995, 2506
AgeI ACCGGT 2 cut(s) 432, 2553
AgsI TTSAA 8 cut(s) 94, 509, 707, 782, 1562, 1801, 1876, 2590
AjiI CACGTC 1 cut(s) 1998
AjnI CCWGG 5 cut(s) 443, 1564, 2996, 3124, 3198
AjuI GAANNNNNNNTTGG 2 cut(s) 2606, 2638
AleI CACNNNNGTG 1 cut(s) 3062
AloI GAACNNNNNNTCC 2 cut(s) 1730, 1762
Alw21I GWGCWC 1 cut(s) 2725
Alw26I GTCTC 4 cut(s) 106, 1212, 2009, 2422
Alw44I GTGCAC 1 cut(s) 2721
AlwI GGATC 8 cut(s) 474, 561, 909, 1815, 1998, 2108, 2265, 3061
AlwNI CAGNNNCTG 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 3095
Aor51HI AGCGCT 1 cut(s) 2970
AoxI GGCC 8 cut(s) 341, 1087, 1291, 1454, 1849, 2493, 2803, 3201
ApaI GGGCCC 1 cut(s) 345
ApaLI GTGCAC 1 cut(s) 2721
ApoI RAATTY 3 cut(s) 1900, 2297, 3154
ArsI GACNNNNNNTTYG 4 cut(s) 151, 183, 3083, 3115
AseI ATTAAT 1 cut(s) 2562
AsiGI ACCGGT 2 cut(s) 432, 2553
Asp700I GAANNNNTTC 1 cut(s) 711
AspLEI GCGC 4 cut(s) 1054, 1530, 2233, 2971
AspS9I GGNCC 8 cut(s) 341, 342, 817, 851, 1303, 1541, 1613, 1849
AsuC2I CCSGG 2 cut(s) 273, 1081
AsuHPI GGTGA 8 cut(s) 674, 2046, 2064, 2401, 2434, 2543, 2918, 3076
AsuII TTCGAA 1 cut(s) 2894
AsuNHI GCTAGC 1 cut(s) 3169
AvaI CYCGRG 1 cut(s) 3095
AvaII GGWCC 5 cut(s) 817, 851, 1303, 1541, 1613
AxyI CCTNAGG 1 cut(s) 1617
BaeGI GKGCMC 2 cut(s) 345, 2725
BalI TGGCCA 2 cut(s) 1456, 2805
BanII GRGCYC 2 cut(s) 345, 3303
BauI CACGAG 1 cut(s) 1840
BbrPI CACGTG 1 cut(s) 844
BbsI GAAGAC 3 cut(s) 1360, 2773, 2802
Bbv12I GWGCWC 1 cut(s) 2725
BceAI ACGGC 1 cut(s) 2304
BciT130I CCWGG 5 cut(s) 445, 1566, 2998, 3126, 3200
BciVI GTATCC 3 cut(s) 141, 1143, 3170
BclI TGATCA 1 cut(s) 966
BcnI CCSGG 2 cut(s) 273, 1081
BcoDI GTCTC 4 cut(s) 106, 1212, 2009, 2422
BfaI CTAG 9 cut(s) 347, 579, 663, 828, 1215, 1266, 1773, 2403, 3170
BfmI CTRYAG 6 cut(s) 669, 1881, 1974, 2157, 2964, 3240
BfoI RGCGCY 1 cut(s) 2972
BfuAI ACCTGC 2 cut(s) 286, 3174
BfuI GTATCC 3 cut(s) 141, 1143, 3170
BlpI GCTNAGC 1 cut(s) 245
Bme1390I CCNGG 7 cut(s) 273, 445, 1081, 1566, 2998, 3126, 3200
Bme18I GGWCC 5 cut(s) 817, 851, 1303, 1541, 1613
BmeT110I CYCGRG 1 cut(s) 3095
BmgBI CACGTC 1 cut(s) 1998
BmgT120I GGNCC 8 cut(s) 341, 342, 817, 851, 1303, 1541, 1613, 1849
BmiI GGNNCC 7 cut(s) 342, 343, 344, 1304, 1614, 3002, 3210
BmrFI CCNGG 7 cut(s) 273, 445, 1081, 1566, 2998, 3126, 3200
BmsI GCATC 5 cut(s) 123, 241, 1164, 2692, 2965
BmtI GCTAGC 1 cut(s) 3173
BpiI GAAGAC 3 cut(s) 1360, 2773, 2802
BplI GAGNNNNNCTC 4 cut(s) 2059, 2091, 2078, 2110
BpmI CTGGAG 2 cut(s) 709, 1947
Bpu1102I GCTNAGC 1 cut(s) 245
Bpu14I TTCGAA 1 cut(s) 2894
BpuEI CTTGAG 1 cut(s) 1316
BpuMI CCSGG 2 cut(s) 273, 1081
Bsa29I ATCGAT 1 cut(s) 219
BsaAI YACGTR 2 cut(s) 844, 2253
BsaI GGTCTC 1 cut(s) 2009
BsaJI CCNNGG 7 cut(s) 765, 1701, 1754, 3032, 3124, 3198, 3204
BsaWI WCCGGW 3 cut(s) 432, 2553, 2617
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bse118I RCCGGY 2 cut(s) 432, 2553
Bse1I ACTGG 4 cut(s) 458, 2166, 2806, 3102
Bse21I CCTNAGG 1 cut(s) 1617
Bse3DI GCAATG 4 cut(s) 752, 1276, 2315, 2337
BseBI CCWGG 5 cut(s) 445, 1566, 2998, 3126, 3200
BseCI ATCGAT 1 cut(s) 219
BseDI CCNNGG 7 cut(s) 765, 1701, 1754, 3032, 3124, 3198, 3204
BseGI GGATG 8 cut(s) 147, 212, 1179, 1351, 1527, 1810, 2822, 3021
BseMI GCAATG 4 cut(s) 752, 1276, 2315, 2337
BseMII CTCAG 5 cut(s) 236, 1205, 1608, 1665, 2297
BseNI ACTGG 4 cut(s) 458, 2166, 2806, 3102
BseRI GAGGAG 2 cut(s) 242, 413
BseSI GKGCMC 2 cut(s) 345, 2725
BseX3I CGGCCG 1 cut(s) 2493
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 2 cut(s) 305, 2496
BshFI GGCC 8 cut(s) 343, 1089, 1293, 1456, 1851, 2495, 2805, 3203
BshTI ACCGGT 2 cut(s) 432, 2553
BshVI ATCGAT 1 cut(s) 219
BsiEI CGRYCG 2 cut(s) 305, 2496
BsiHKAI GWGCWC 1 cut(s) 2725
BsiHKCI CYCGRG 1 cut(s) 3095
BsiSI CCGG 5 cut(s) 272, 433, 1080, 2554, 2618
BslFI GGGAC 4 cut(s) 1096, 1316, 1626, 2966
BsmAI GTCTC 4 cut(s) 106, 1212, 2009, 2422
BsmBI CGTCTC 3 cut(s) 106, 1212, 2422
BsmFI GGGAC 4 cut(s) 1096, 1316, 1626, 2966
BsmI GAATGC 1 cut(s) 1530
BsnI GGCC 8 cut(s) 343, 1089, 1293, 1456, 1851, 2495, 2805, 3203
Bso31I GGTCTC 1 cut(s) 2009
BsoBI CYCGRG 1 cut(s) 3095
Bsp119I TTCGAA 1 cut(s) 2894
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 3 cut(s) 345, 2725, 3303
Bsp1407I TGTACA 3 cut(s) 1245, 2025, 2503
Bsp1720I GCTNAGC 1 cut(s) 245
Bsp19I CCATGG 2 cut(s) 1701, 3032
BspACI CCGC 8 cut(s) 74, 249, 282, 338, 1254, 1766, 2492, 2496
BspANI GGCC 8 cut(s) 343, 1089, 1293, 1456, 1851, 2495, 2805, 3203
BspCNI CTCAG 5 cut(s) 237, 1204, 1609, 1664, 2298
BspDI ATCGAT 1 cut(s) 219
BspFNI CGCG 1 cut(s) 74
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 7 cut(s) 342, 343, 344, 1304, 1614, 3002, 3210
BspMAI CTGCAG 4 cut(s) 673, 1885, 2161, 2968
BspMI ACCTGC 2 cut(s) 286, 3174
BspOI GCTAGC 1 cut(s) 3173
BspPI GGATC 8 cut(s) 474, 561, 909, 1815, 1998, 2108, 2265, 3061
BspT104I TTCGAA 1 cut(s) 2894
BspTNI GGTCTC 1 cut(s) 2009
BsrBI CCGCTC 1 cut(s) 249
BsrDI GCAATG 4 cut(s) 752, 1276, 2315, 2337
BsrFI RCCGGY 2 cut(s) 432, 2553
BsrGI TGTACA 3 cut(s) 1245, 2025, 2503
BsrI ACTGG 4 cut(s) 458, 2166, 2806, 3102
BssAI RCCGGY 2 cut(s) 432, 2553
BssECI CCNNGG 7 cut(s) 765, 1701, 1754, 3032, 3124, 3198, 3204
BssSI CACGAG 1 cut(s) 1840
BssT1I CCWWGG 5 cut(s) 765, 1701, 1754, 3032, 3204
Bst2BI CACGAG 1 cut(s) 1840
Bst2UI CCWGG 5 cut(s) 445, 1566, 2998, 3126, 3200
Bst6I CTCTTC 3 cut(s) 381, 2896, 3199
BstAPI GCANNNNNTGC 1 cut(s) 2639
BstAUI TGTACA 3 cut(s) 1245, 2025, 2503
BstBAI YACGTR 2 cut(s) 844, 2253
BstBI TTCGAA 1 cut(s) 2894
BstC8I GCNNGC 8 cut(s) 174, 309, 1091, 1584, 1885, 1946, 2656, 3171
BstDEI CTNAG 6 cut(s) 245, 1064, 1191, 1617, 1651, 2306
BstDSI CCRYGG 2 cut(s) 1701, 3032
BstF5I GGATG 8 cut(s) 147, 212, 1179, 1351, 1527, 1810, 2822, 3021
BstFNI CGCG 1 cut(s) 74
BstH2I RGCGCY 1 cut(s) 2972
BstHHI GCGC 4 cut(s) 1054, 1530, 2233, 2971
BstMAI GTCTC 4 cut(s) 106, 1212, 2009, 2422
BstMCI CGRYCG 2 cut(s) 305, 2496
BstMWI GCNNNNNNNGC 8 cut(s) 349, 668, 978, 1954, 2239, 2639, 2811, 3252
BstNI CCWGG 5 cut(s) 445, 1566, 2998, 3126, 3200
BstNSI RCATGY 5 cut(s) 130, 518, 1263, 1627, 2510
BstSCI CCNGG 7 cut(s) 271, 443, 1079, 1564, 2996, 3124, 3198
BstSFI CTRYAG 6 cut(s) 669, 1881, 1974, 2157, 2964, 3240
BstSLI GKGCMC 2 cut(s) 345, 2725
BstUI CGCG 1 cut(s) 74
BstV2I GAAGAC 3 cut(s) 1360, 2773, 2802
BstX2I RGATCY 4 cut(s) 466, 914, 2113, 2257
BstXI CCANNNNNNTGG 2 cut(s) 1846, 2064
BstYI RGATCY 4 cut(s) 466, 914, 2113, 2257
BstZI CGGCCG 1 cut(s) 2493
Bsu15I ATCGAT 1 cut(s) 219
Bsu36I CCTNAGG 1 cut(s) 1617
BsuI GTATCC 3 cut(s) 141, 1143, 3170
BsuRI GGCC 8 cut(s) 343, 1089, 1293, 1456, 1851, 2495, 2805, 3203
BsuTUI ATCGAT 1 cut(s) 219
BtgI CCRYGG 2 cut(s) 1701, 3032
BtrI CACGTC 1 cut(s) 1998
BtsCI GGATG 8 cut(s) 147, 212, 1179, 1351, 1527, 1810, 2822, 3021
BtsI GCAGTG 1 cut(s) 38
BtsIMutI CAGTG 4 cut(s) 38, 1329, 2035, 2725
BveI ACCTGC 2 cut(s) 286, 3174
Cac8I GCNNGC 8 cut(s) 174, 309, 1091, 1584, 1885, 1946, 2656, 3171
CaiI CAGNNNCTG 1 cut(s) 320
CciI TCATGA 1 cut(s) 117
CciNI GCGGCCGC 1 cut(s) 2493
CfoI GCGC 4 cut(s) 1054, 1530, 2233, 2971
Cfr10I RCCGGY 2 cut(s) 432, 2553
Cfr13I GGNCC 8 cut(s) 341, 342, 817, 851, 1303, 1541, 1613, 1849
ClaI ATCGAT 1 cut(s) 219
CspAI ACCGGT 2 cut(s) 432, 2553
CspCI CAANNNNNGTGG 2 cut(s) 2631, 2666
DdeI CTNAG 6 cut(s) 245, 1064, 1191, 1617, 1651, 2306
DraI TTTAAA 1 cut(s) 19
EaeI YGGCCR 3 cut(s) 1454, 2493, 2803
EagI CGGCCG 1 cut(s) 2493
Eam1104I CTCTTC 3 cut(s) 381, 2896, 3199
EarI CTCTTC 3 cut(s) 381, 2896, 3199
EclXI CGGCCG 1 cut(s) 2493
Eco130I CCWWGG 5 cut(s) 765, 1701, 1754, 3032, 3204
Eco24I GRGCYC 2 cut(s) 345, 3303
Eco31I GGTCTC 1 cut(s) 2009
Eco32I GATATC 2 cut(s) 332, 2472
Eco47I GGWCC 5 cut(s) 817, 851, 1303, 1541, 1613
Eco47III AGCGCT 1 cut(s) 2970
Eco52I CGGCCG 1 cut(s) 2493
Eco57I CTGAAG 4 cut(s) 257, 405, 1461, 2883
Eco72I CACGTG 1 cut(s) 844
Eco81I CCTNAGG 1 cut(s) 1617
Eco88I CYCGRG 1 cut(s) 3095
EcoO109I RGGNCCY 4 cut(s) 341, 342, 1303, 1613
EcoRI GAATTC 1 cut(s) 1900
EcoRII CCWGG 5 cut(s) 443, 1564, 2996, 3124, 3198
EcoRV GATATC 2 cut(s) 332, 2472
EcoT14I CCWWGG 5 cut(s) 765, 1701, 1754, 3032, 3204
EcoT38I GRGCYC 2 cut(s) 345, 3303
ErhI CCWWGG 5 cut(s) 765, 1701, 1754, 3032, 3204
Esp3I CGTCTC 3 cut(s) 106, 1212, 2422
FaqI GGGAC 4 cut(s) 1096, 1316, 1626, 2966
FauI CCCGC 2 cut(s) 67, 331
FbaI TGATCA 1 cut(s) 966
FblI GTMKAC 1 cut(s) 1074
FokI GGATG 8 cut(s) 134, 219, 1186, 1358, 1534, 1797, 2809, 3028
FriOI GRGCYC 2 cut(s) 345, 3303
FspAI RTGCGCAY 1 cut(s) 1529
FspBI CTAG 9 cut(s) 347, 579, 663, 828, 1215, 1266, 1773, 2403, 3170
FspI TGCGCA 1 cut(s) 1529
GlaI GCGC 4 cut(s) 1053, 1529, 2232, 2970
GsuI CTGGAG 2 cut(s) 709, 1947
HaeII RGCGCY 1 cut(s) 2972
HaeIII GGCC 8 cut(s) 343, 1089, 1293, 1456, 1851, 2495, 2805, 3203
HapII CCGG 5 cut(s) 272, 433, 1080, 2554, 2618
HhaI GCGC 4 cut(s) 1054, 1530, 2233, 2971
Hin6I GCGC 4 cut(s) 1052, 1528, 2231, 2969
HinP1I GCGC 4 cut(s) 1052, 1528, 2231, 2969
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HindIII AAGCTT 3 cut(s) 369, 1584, 1955
HpaII CCGG 5 cut(s) 272, 433, 1080, 2554, 2618
HphI GGTGA 8 cut(s) 674, 2046, 2064, 2401, 2434, 2543, 2918, 3076
Hpy166II GTNNAC 7 cut(s) 459, 532, 1075, 1248, 2072, 2551, 2723
Hpy8I GTNNAC 7 cut(s) 459, 532, 1075, 1248, 2072, 2551, 2723
Hpy99I CGWCG 1 cut(s) 50
HpyCH4IV ACGT 6 cut(s) 122, 784, 843, 1221, 1997, 2252
HpyF10VI GCNNNNNNNGC 8 cut(s) 349, 668, 978, 1954, 2239, 2639, 2811, 3252
HpyF3I CTNAG 6 cut(s) 245, 1064, 1191, 1617, 1651, 2306
HpySE526I ACGT 6 cut(s) 122, 784, 843, 1221, 1997, 2252
HspAI GCGC 4 cut(s) 1052, 1528, 2231, 2969
Ksp22I TGATCA 1 cut(s) 966
LmnI GCTCC 5 cut(s) 229, 1429, 1579, 2879, 3214
LweI GCATC 5 cut(s) 123, 241, 1164, 2692, 2965
MaeI CTAG 9 cut(s) 347, 579, 663, 828, 1215, 1266, 1773, 2403, 3170
MaeII ACGT 6 cut(s) 122, 784, 843, 1221, 1997, 2252
MbiI CCGCTC 1 cut(s) 249
MflI RGATCY 4 cut(s) 466, 914, 2113, 2257
MhlI GDGCHC 3 cut(s) 345, 2725, 3303
MlsI TGGCCA 2 cut(s) 1456, 2805
MluNI TGGCCA 2 cut(s) 1456, 2805
MlyI GAGTC 3 cut(s) 154, 1070, 3107
MmeI TCCRAC 5 cut(s) 429, 903, 1691, 2901, 3260
Mox20I TGGCCA 2 cut(s) 1456, 2805
MroXI GAANNNNTTC 1 cut(s) 711
MscI TGGCCA 2 cut(s) 1456, 2805
MseI TTAA 8 cut(s) 18, 377, 420, 789, 1722, 2301, 2562, 2606
MslI CAYNNNNRTG 4 cut(s) 1281, 1409, 2937, 3062
Msp20I TGGCCA 2 cut(s) 1456, 2805
MspA1I CMGCKG 3 cut(s) 1256, 2162, 2498
MspI CCGG 5 cut(s) 272, 433, 1080, 2554, 2618
MspR9I CCNGG 7 cut(s) 273, 445, 1081, 1566, 2998, 3126, 3200
Mva1269I GAATGC 1 cut(s) 1530
MvaI CCWGG 5 cut(s) 445, 1566, 2998, 3126, 3200
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 8 cut(s) 349, 668, 978, 1954, 2239, 2639, 2811, 3252
NciI CCSGG 2 cut(s) 273, 1081
NcoI CCATGG 2 cut(s) 1701, 3032
NheI GCTAGC 1 cut(s) 3169
NlaIV GGNNCC 7 cut(s) 342, 343, 344, 1304, 1614, 3002, 3210
NmuCI GTSAC 9 cut(s) 409, 638, 770, 1327, 1411, 1510, 2422, 2714, 2985
NotI GCGGCCGC 1 cut(s) 2493
NsbI TGCGCA 1 cut(s) 1529
NspI RCATGY 5 cut(s) 130, 518, 1263, 1627, 2510
NspV TTCGAA 1 cut(s) 2894
OliI CACNNNNGTG 1 cut(s) 3062
PagI TCATGA 1 cut(s) 117
PciI ACATGT 2 cut(s) 126, 2506
PctI GAATGC 1 cut(s) 1530
PdmI GAANNNNTTC 1 cut(s) 711
PfoI TCCNGGA 2 cut(s) 271, 1079
PinAI ACCGGT 2 cut(s) 432, 2553
Ple19I CGATCG 1 cut(s) 305
PleI GAGTC 3 cut(s) 154, 1070, 3106
PmaCI CACGTG 1 cut(s) 844
PmlI CACGTG 1 cut(s) 844
PpsI GAGTC 3 cut(s) 154, 1070, 3106
Ppu21I YACGTR 2 cut(s) 844, 2253
PpuMI RGGWCCY 2 cut(s) 1303, 1613
PscI ACATGT 2 cut(s) 126, 2506
PshBI ATTAAT 1 cut(s) 2562
PsiI TTATAA 4 cut(s) 573, 897, 1785, 2687
Psp5II RGGWCCY 2 cut(s) 1303, 1613
Psp6I CCWGG 5 cut(s) 443, 1564, 2996, 3124, 3198
PspCI CACGTG 1 cut(s) 844
PspGI CCWGG 5 cut(s) 443, 1564, 2996, 3124, 3198
PspN4I GGNNCC 7 cut(s) 342, 343, 344, 1304, 1614, 3002, 3210
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 8 cut(s) 341, 342, 817, 851, 1303, 1541, 1613, 1849
PspPPI RGGWCCY 2 cut(s) 1303, 1613
PstI CTGCAG 4 cut(s) 673, 1885, 2161, 2968
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 4 cut(s) 466, 914, 2113, 2257
PvuI CGATCG 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 2162
RseI CAYNNNNRTG 4 cut(s) 1281, 1409, 2937, 3062
SaqAI TTAA 8 cut(s) 18, 377, 420, 789, 1722, 2301, 2562, 2606
Sau96I GGNCC 8 cut(s) 341, 342, 817, 851, 1303, 1541, 1613, 1849
SchI GAGTC 3 cut(s) 154, 1070, 3107
ScrFI CCNGG 7 cut(s) 273, 445, 1081, 1566, 2998, 3126, 3200
SduI GDGCHC 3 cut(s) 345, 2725, 3303
SfaNI GCATC 5 cut(s) 123, 241, 1164, 2692, 2965
SfcI CTRYAG 6 cut(s) 669, 1881, 1974, 2157, 2964, 3240
SfuI TTCGAA 1 cut(s) 2894
SinI GGWCC 5 cut(s) 817, 851, 1303, 1541, 1613
SmiMI CAYNNNNRTG 4 cut(s) 1281, 1409, 2937, 3062
SmlI CTYRAG 1 cut(s) 1295
SmoI CTYRAG 1 cut(s) 1295
SsiI CCGC 8 cut(s) 74, 249, 282, 338, 1254, 1766, 2492, 2496
SspI AATATT 1 cut(s) 2854
SspMI CTAG 9 cut(s) 347, 579, 663, 828, 1215, 1266, 1773, 2403, 3170
StyD4I CCNGG 7 cut(s) 271, 443, 1079, 1564, 2996, 3124, 3198
StyI CCWWGG 5 cut(s) 765, 1701, 1754, 3032, 3204
TaiI ACGT 6 cut(s) 125, 787, 846, 1224, 2000, 2255
TaqII GACCGA 1 cut(s) 2666
TatI WGTACW 4 cut(s) 974, 1245, 2025, 2503
TauI GCSGC 5 cut(s) 252, 285, 1768, 2495, 2498
Tru1I TTAA 8 cut(s) 18, 377, 420, 789, 1722, 2301, 2562, 2606
Tru9I TTAA 8 cut(s) 18, 377, 420, 789, 1722, 2301, 2562, 2606
TscAI CASTG 4 cut(s) 45, 1336, 2035, 2725
TseFI GTSAC 9 cut(s) 409, 638, 770, 1327, 1411, 1510, 2422, 2714, 2985
Tsp45I GTSAC 9 cut(s) 409, 638, 770, 1327, 1411, 1510, 2422, 2714, 2985
TspGWI ACGGA 3 cut(s) 1416, 1592, 3201
TspRI CASTG 4 cut(s) 45, 1336, 2035, 2725
VneI GTGCAC 1 cut(s) 2721
VpaK11BI GGWCC 5 cut(s) 817, 851, 1303, 1541, 1613
VspI ATTAAT 1 cut(s) 2562
XapI RAATTY 3 cut(s) 1900, 2297, 3154
XceI RCATGY 5 cut(s) 130, 518, 1263, 1627, 2510
XcmI CCANNNNNNNNNTGG 2 cut(s) 2936, 3109
XmiI GTMKAC 1 cut(s) 1074
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 9 cut(s) 347, 579, 663, 828, 1215, 1266, 1773, 2403, 3170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.