Rw2G053710

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005447
Physical Location & Seq
Reverse (-)
81421381 .. 81429175
7795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053710.1

Sequence Viewer

Length: 3348 bp
ATGGAGGTGCTAGGGTTCAAAGCGAAGTCGAAAATAGCACTGCTCGTTGTTCTCGTGCTGAGCTTTGGCTTCGTGGCGCTGTATGGCTTGTTGAAGCCGGTTTCGAACGGCTGTAACATGACGTATATGTATCCGACGTATATTCCGATACAGATGACTAACTCCGGTGCTGCACCGGCGGCGAAGTATGGGCTGTATTTGTACCACGAAGGCTGGAAGACGATTGATTTTAAGGAGCATGTACAGAAGCTAAGCGGCATTCCGGTGCTTTTTATTCCGGGGAATGGCGGGAGCTATAAGCAGGTGAGGTCATTGGCTGCGGAGTCTGATAGGGCGTATAAAGTAGGTCCACTTGAGCGTACGTTTTATGAAGAAGCTCGGTTAACTCCGGAAGAGGGAGGGGAGGAGGATATGGATGCAGCTGGCTTTGAGTTGAAGAACCAATACGATTCGAGGTTGGACTGGTTCGCTGTGGATCTTGAAGGAGAACACTCTGCTATGGATAGTGCTGTACTTGAACAACACACACAATATGTTGTCCACTGCATTCACAGGATTTTGGATCAGTACGAGGAGTCTTTCAAGGCTAGAGAAAAGGAAGGAGCTGCAACCTCTGCGACTTTGCCAAAAAGTGTTATATTGGTTGGTCACTCTATGGGTGGTTTCGTTGCTAGAGCTGCAGTGGTCGACCCTCTTTTGAGAAAGTCAGCAGTTGAAACAATTATTACACTTTCCAGCCCACACCAACAACCTCCTGTGGCACTGCAGCCTTCCTACGGTCACTACTTTAGACATGTTAATCGAGAATGGAGAAAGGGATATGAGGTCCAAACAACTGCAGCAGGACATTACTTGTCTGGTCCTGCTCTCTCTAATGTTGTTGTCATATCTATTTCTGGTGGTTATAATGATTACCAGGTAAGGACCAAGTATGAATCCCTTGATGGCATTGTTCCGCCTACCCATGGTTTTATGATCAGTAGTACCGGCATGAAAAATGTTTGGTTATCCATGGAACATCAAGTTATCTTATGGTGCAATCAATTAGTTATCCAAGTATCACACACTCTCCTTAGTTTGGCAGATTCCAGAACAGGCCAGCCTTTCTCTGACACACGAATAAGACTTGCAATATTCTCGAGAATGCTTCGTAGTGCAATACCACAAAGTTTCAATTGGAGGAAGCAATTGCAATTGTCTCAACAGTCAATGCATACTCCAACTAGAGACGTGAAAGACAGAACTGAATCACTATACATCCCTGCTACTTGTCCAAGCAATGTTCATTGGAGTGATGATGGCCTTGAGAGGGACTTGTACATTCAGACAACCACCGTCACTGTTTTAGCCATGGATGGTCGAAGACGGTGGTTGGACATTCAAAAACTGGGATCCAATGGAAGAAGTCACTTCATGTTTGTGACCAACCTTGCTCCATGTTCTGGGGTTAGACTGCATCTGTGGCCTGACAAAAGAAACTCAACTTCAGACTTGCCTGCATGTATAAGGATCCATGAGGTGACATCGAAGATGGTGCTCATTCCATCAGGACCAGCACCAAGACAGATTGAACCAGGAAGCCAAACAGAGCAAGCTCCCCCTTCAGCTATACTCCGCTTAGGACCCAAGGATATGCGTGGTTTCAGATTCCTGACCATCTCAGTTGCACCACGGCCGAGTATTTCAGGCAGACCTCCACCAGCAGTTTCCATGGCAGTTGGTCAGTTTTTTAATCCATCAGCAGGGGAGATAGAGATCTCTCCTTGGTCACCTCCACTGTCTGGTTATTCTCACAAGGATTTATCTTGGAAGGAAGATCATCCCCTTGCTTTGAATCTATCATTCACAAGTAGTTTAGGCCTTCTGCCTGTAGAGTTCTCTTTGAAGACCGCAGGTTGTGGAATAAAGAGTTCTGGACTTCCAGATGAACAGGCTGGAGATATCGATAATAGCAAGCTCTGTAAGCTTCGCTGTTTCCCACCAGTTGCATTTGCTTGGGATGAAACAGCAGGTCTCCACGTATTCACAGATTTGTATAGTGACAAAATTGTTGTTGATTCATCACCATCGCTTTGGAGTTCACCCAGAAGTTCTGAGAAAACCTCTGTTATGTTGGTGGTAGATCCACATTGTTCATATAGCAGTGCGATGACTGTTTCCGTAACTGCAGCTGCCAGTAGGTTTTTGCTTGTGTATACTTCACAGATTATTGGTTTCTCCATTGTTGTTATCTTTTTTGCACTGATGCGGCAAGCATGTGCATGGGATCGTAACCTACCCATACCCTCGATGTTGAAGGCTCTGGAGTCTAATTTGAGATTCCCAATTCCATTTATCTACCTTGCTATTGCACCAATTGGTCTTTCTTTACTCGTTGGTTTTTGGATTTCTCAACCATCTCCTTCATTTGCTAGCTTCACCATTGTATCAGTTGTCTGCTATTTCCTTGCTAATGGCTTCATAGCCATTCTGATTTTGACATCCCAGTTCGCCTTTTATGGGGCTGCTCTGCTACATATTTTCATCAAGAAAAGGTTTCAATTGGGGGGGAAAACTTCTCAATGGTTTGTGAATATATCCTCTGGTTTCTGTTCATGGAAGGTGGTAAGGGTTATAAGAGCTAATCCACTAATGGTTACAGCACTTGCTGCAATTAGTTTGGCATGCTTGGTTCATCCGGGTTTGGGTCTACTTGTTCTCTTGCTCTCTCATGCTTTGTGCTGTCATACTGCTCTTTGCAGTTTTTTGACAGCTTCATTTCGCAGCCATGCACGAAGACATGAATTATTTGATTATAAGAAAGACGGAAATGGTGGGCCTGACAAACTTACATTCAAACGTGATGGTATATCAAACCAGAATTTTTCTTCTGAAGATACGTGCTCCAATAGCCCAGACTCTCCAAAAAGTTTTGGTGAAACTCAATTAGAGATATTCCATCATCGGCATGGGTTGTTTATTTTGCATCTTGTTGCAACGCTCATGTTTGGCCCATCATTTGTGACTTGGTTGGAGAGAATTGGGACGGATCATAGCTTTCCATGGGTCTTGGATTCAGCTCTTTCCACGGGTGTGATCCTTCATGGTATCTTCACTTCAAACCCTCAGTTCAATTCCTTCCTGGTTTCTTTCCCTCCAATCCGAAACCTAGAAGTGAGGATGCATTTACTGTACCTGTTTGCTGGATACTATTCCTATCTCTCTTCCCTGGCCTTGGCTCCATATAGAGAATTTTATGTCATGGCTGCTGTAGGCTATACTTCAATTGGTCTGACCCTCTTACAGAGATGGAACAAGGAGAAGGGAGATGCCCATTTTGTTAACCGAAAGCACTCTCACAGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

1115

Amino Acids

123.9

Weight (kDa)

8.76

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGAP1 PF07819 84 - 359 3.2e-82 PGAP1-like alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 906, 2615, 2796
AarI CACCTGC 1 cut(s) 292
Acc36I ACCTGC 3 cut(s) 292, 1883, 2000
AccB7I CCANNNNNTGG 2 cut(s) 1442, 1781
AccI GTMKAC 3 cut(s) 687, 2195, 2689
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 8 cut(s) 179, 255, 288, 320, 956, 1615, 1890, 2248
AcoI YGGCCR 1 cut(s) 1673
AcsI RAATTY 2 cut(s) 2860, 3230
AcuI CTGAAG 3 cut(s) 1470, 1587, 2892
AfaI GTAC 8 cut(s) 203, 243, 361, 513, 569, 985, 1319, 3173
AflIII ACRYGT 1 cut(s) 793
AjiI CACGTC 1 cut(s) 1231
AjnI CCWGG 4 cut(s) 915, 1573, 3120, 3207
AjuI GAANNNNNNNTTGG 2 cut(s) 2347, 2379
AleI CACNNNNGTG 1 cut(s) 3071
Alw21I GWGCWC 2 cut(s) 1539, 2885
Alw26I GTCTC 3 cut(s) 1203, 1221, 2018
AlwNI CAGNNNCTG 1 cut(s) 3345
Ama87I CYCGRG 1 cut(s) 1138
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 8 cut(s) 1096, 1300, 1463, 1673, 1858, 2816, 2989, 3210
ApoI RAATTY 2 cut(s) 2860, 3230
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
AsuC2I CCSGG 2 cut(s) 279, 2679
AsuHPI GGTGA 7 cut(s) 316, 1531, 1761, 2055, 2073, 2410, 2927
AsuII TTCGAA 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 2411
AvaI CYCGRG 1 cut(s) 1138
AvaII GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
BaeI ACNNNNGTAYC 2 cut(s) 2409, 2442
BamHI GGATCC 2 cut(s) 1391, 1509
BarI GAAGNNNNNNTAC 4 cut(s) 428, 460, 3079, 3111
BauI CACGAG 1 cut(s) 53
BbsI GAAGAC 4 cut(s) 224, 1369, 1892, 2782
Bbv12I GWGCWC 2 cut(s) 1539, 2885
BceAI ACGGC 2 cut(s) 124, 1688
BcgI CGANNNNNNTGC 4 cut(s) 1119, 1153, 1516, 1550
BciT130I CCWGG 4 cut(s) 917, 1575, 3122, 3209
BciVI GTATCC 2 cut(s) 141, 3179
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 2 cut(s) 279, 2679
BcoDI GTCTC 3 cut(s) 1203, 1221, 2018
BfaI CTAG 6 cut(s) 11, 588, 672, 1224, 2412, 3149
BfmI CTRYAG 6 cut(s) 678, 764, 837, 1869, 2166, 3249
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 3 cut(s) 292, 1883, 2000
BfuI GTATCC 2 cut(s) 141, 3179
BglII AGATCT 1 cut(s) 1755
BlpI GCTNAGC 2 cut(s) 59, 251
Bme1390I CCNGG 6 cut(s) 279, 917, 1575, 2679, 3122, 3209
Bme18I GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
BmeT110I CYCGRG 1 cut(s) 1138
BmgBI CACGTC 1 cut(s) 1231
BmgT120I GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
BmiI GGNNCC 4 cut(s) 1393, 1511, 1624, 3219
BmrFI CCNGG 6 cut(s) 279, 917, 1575, 2679, 3122, 3209
BmrI ACTGGG 2 cut(s) 1397, 2479
BmsI GCATC 6 cut(s) 406, 1465, 2235, 2974, 3150, 3298
BmtI GCTAGC 1 cut(s) 2415
BmuI ACTGGG 2 cut(s) 1397, 2479
BpiI GAAGAC 4 cut(s) 224, 1369, 1892, 2782
BplI GAGNNNNNCTC 2 cut(s) 2087, 2119
BpmI CTGGAG 2 cut(s) 1956, 2324
Bpu10I CCTNAGC 1 cut(s) 1618
Bpu1102I GCTNAGC 2 cut(s) 59, 251
Bpu14I TTCGAA 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 374, 1325
BpuMI CCSGG 2 cut(s) 279, 2679
Bsa29I ATCGAT 1 cut(s) 1944
BsaAI YACGTR 2 cut(s) 2020, 2880
BsaBI GATNNNNATC 1 cut(s) 1754
BsaI GGTCTC 1 cut(s) 2018
BsaWI WCCGGW 3 cut(s) 164, 262, 388
BsaXI ACNNNNNCTCC 2 cut(s) 1053, 1083
Bse118I RCCGGY 3 cut(s) 97, 175, 986
Bse1I ACTGG 5 cut(s) 467, 1392, 1982, 2175, 2485
Bse3DI GCAATG 1 cut(s) 1285
Bse8I GATNNNNATC 1 cut(s) 1754
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 4 cut(s) 917, 1575, 3122, 3209
BseCI ATCGAT 1 cut(s) 1944
BseGI GGATG 8 cut(s) 421, 1257, 1360, 1819, 2005, 2480, 2674, 3165
BseJI GATNNNNATC 1 cut(s) 1754
BseMI GCAATG 1 cut(s) 1285
BseMII CTCAG 4 cut(s) 50, 1674, 2085, 3119
BseNI ACTGG 5 cut(s) 467, 1392, 1982, 2175, 2485
BseRI GAGGAG 2 cut(s) 419, 587
BseX3I CGGCCG 1 cut(s) 1673
BsgI GTGCAG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 1676
BshFI GGCC 8 cut(s) 1098, 1302, 1465, 1675, 1860, 2818, 2991, 3212
BshVI ATCGAT 1 cut(s) 1944
BsiEI CGRYCG 1 cut(s) 1676
BsiHKAI GWGCWC 2 cut(s) 1539, 2885
BsiHKCI CYCGRG 1 cut(s) 1138
BsiSI CCGG 8 cut(s) 98, 165, 176, 263, 278, 389, 987, 2678
BsiWI CGTACG 1 cut(s) 359
BslFI GGGAC 2 cut(s) 1325, 3037
BsmAI GTCTC 3 cut(s) 1203, 1221, 2018
BsmBI CGTCTC 1 cut(s) 1221
BsmFI GGGAC 2 cut(s) 1325, 3037
BsmI GAATGC 3 cut(s) 258, 546, 1149
BsnI GGCC 8 cut(s) 1098, 1302, 1465, 1675, 1860, 2818, 2991, 3212
Bso31I GGTCTC 1 cut(s) 2018
BsoBI CYCGRG 1 cut(s) 1138
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 1539, 2885
Bsp13I TCCGGA 1 cut(s) 388
Bsp1407I TGTACA 2 cut(s) 241, 1317
Bsp1720I GCTNAGC 2 cut(s) 59, 251
Bsp19I CCATGG 5 cut(s) 964, 1011, 1350, 1710, 3041
BspACI CCGC 8 cut(s) 179, 255, 288, 320, 956, 1615, 1890, 2248
BspANI GGCC 8 cut(s) 1098, 1302, 1465, 1675, 1860, 2818, 2991, 3212
BspCNI CTCAG 4 cut(s) 51, 1673, 2086, 3118
BspDI ATCGAT 1 cut(s) 1944
BspEI TCCGGA 1 cut(s) 388
BspLI GGNNCC 4 cut(s) 1393, 1511, 1624, 3219
BspMAI CTGCAG 4 cut(s) 682, 768, 841, 2170
BspMI ACCTGC 3 cut(s) 292, 1883, 2000
BspOI GCTAGC 1 cut(s) 2415
BspT104I TTCGAA 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 2018
BsrDI GCAATG 1 cut(s) 1285
BsrFI RCCGGY 3 cut(s) 97, 175, 986
BsrGI TGTACA 2 cut(s) 241, 1317
BsrI ACTGG 5 cut(s) 467, 1392, 1982, 2175, 2485
BssAI RCCGGY 3 cut(s) 97, 175, 986
BssNAI GTATAC 1 cut(s) 2196
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Bst1107I GTATAC 1 cut(s) 2196
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 4 cut(s) 917, 1575, 3122, 3209
Bst4CI ACNGT 8 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155, 3171
Bst6I CTCTTC 2 cut(s) 387, 3208
BstAPI GCANNNNNTGC 2 cut(s) 614, 2648
BstAUI TGTACA 2 cut(s) 241, 1317
BstBAI YACGTR 2 cut(s) 2020, 2880
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 8 cut(s) 424, 1100, 1497, 1593, 1955, 2253, 2413, 2665
BstDEI CTNAG 7 cut(s) 59, 251, 1073, 1618, 1660, 2094, 3105
BstDSI CCRYGG 7 cut(s) 964, 1011, 1350, 1670, 1710, 3041, 3066
BstEII GGTNACC 1 cut(s) 1767
BstF5I GGATG 8 cut(s) 421, 1257, 1360, 1819, 2005, 2480, 2674, 3165
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstMAI GTCTC 3 cut(s) 1203, 1221, 2018
BstMCI CGRYCG 1 cut(s) 1676
BstMWI GCNNNNNNNGC 8 cut(s) 176, 179, 614, 677, 1462, 1963, 2648, 2889
BstNI CCWGG 4 cut(s) 917, 1575, 3122, 3209
BstNSI RCATGY 5 cut(s) 242, 797, 1503, 2259, 2667
BstPI GGTNACC 1 cut(s) 1767
BstSCI CCNGG 6 cut(s) 277, 915, 1573, 2677, 3120, 3207
BstSFI CTRYAG 6 cut(s) 678, 764, 837, 1869, 2166, 3249
BstV2I GAAGAC 4 cut(s) 224, 1369, 1892, 2782
BstX2I RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
BstXI CCANNNNNNTGG 1 cut(s) 2073
BstYI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
BstZ17I GTATAC 1 cut(s) 2196
BstZI CGGCCG 1 cut(s) 1673
Bsu15I ATCGAT 1 cut(s) 1944
BsuI GTATCC 2 cut(s) 141, 3179
BsuRI GGCC 8 cut(s) 1098, 1302, 1465, 1675, 1860, 2818, 2991, 3212
BsuTUI ATCGAT 1 cut(s) 1944
BtgI CCRYGG 7 cut(s) 964, 1011, 1350, 1670, 1710, 3041, 3066
BtgZI GCGATG 2 cut(s) 2052, 2162
BtrI CACGTC 1 cut(s) 1231
BtsCI GGATG 8 cut(s) 421, 1257, 1360, 1819, 2005, 2480, 2674, 3165
BtsI GCAGTG 5 cut(s) 38, 541, 687, 761, 2149
BtsIMutI CAGTG 9 cut(s) 38, 541, 687, 761, 1338, 1775, 2149, 2240, 3343
BveI ACCTGC 3 cut(s) 292, 1883, 2000
Cac8I GCNNGC 8 cut(s) 424, 1100, 1497, 1593, 1955, 2253, 2413, 2665
CaiI CAGNNNCTG 1 cut(s) 3345
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 3 cut(s) 97, 175, 986
Cfr13I GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
ClaI ATCGAT 1 cut(s) 1944
CsiI ACCWGGT 1 cut(s) 915
Csp6I GTAC 8 cut(s) 202, 242, 360, 512, 568, 984, 1318, 3172
CviQI GTAC 8 cut(s) 202, 242, 360, 512, 568, 984, 1318, 3172
DdeI CTNAG 7 cut(s) 59, 251, 1073, 1618, 1660, 2094, 3105
EaeI YGGCCR 1 cut(s) 1673
EagI CGGCCG 1 cut(s) 1673
Eam1104I CTCTTC 2 cut(s) 387, 3208
EarI CTCTTC 2 cut(s) 387, 3208
EciI GGCGGA 1 cut(s) 945
EclXI CGGCCG 1 cut(s) 1673
Eco130I CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Eco147I AGGCCT 1 cut(s) 1860
Eco31I GGTCTC 1 cut(s) 2018
Eco32I GATATC 1 cut(s) 1942
Eco47I GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
Eco52I CGGCCG 1 cut(s) 1673
Eco57I CTGAAG 3 cut(s) 1470, 1587, 2892
Eco88I CYCGRG 1 cut(s) 1138
Eco91I GGTNACC 1 cut(s) 1767
EcoO109I RGGNCCY 1 cut(s) 1622
EcoO65I GGTNACC 1 cut(s) 1767
EcoRII CCWGG 4 cut(s) 915, 1573, 3120, 3207
EcoRV GATATC 1 cut(s) 1942
EcoT14I CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
EcoT22I ATGCAT 2 cut(s) 1215, 3165
ErhI CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Esp3I CGTCTC 1 cut(s) 1221
FaqI GGGAC 2 cut(s) 1325, 3037
FauI CCCGC 1 cut(s) 281
FbaI TGATCA 1 cut(s) 975
FblI GTMKAC 3 cut(s) 687, 2195, 2689
FokI GGATG 8 cut(s) 428, 1244, 1367, 1806, 2012, 2467, 2661, 3172
FspBI CTAG 6 cut(s) 11, 588, 672, 1224, 2412, 3149
GlaI GCGC 1 cut(s) 78
GsuI CTGGAG 2 cut(s) 1956, 2324
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 8 cut(s) 1098, 1302, 1465, 1675, 1860, 2818, 2991, 3212
HapII CCGG 8 cut(s) 98, 165, 176, 263, 278, 389, 987, 2678
HhaI GCGC 1 cut(s) 79
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 3 cut(s) 384, 688, 3322
HindII GTYRAC 3 cut(s) 384, 688, 3322
HindIII AAGCTT 1 cut(s) 1964
HpaI GTTAAC 2 cut(s) 384, 3322
HpaII CCGG 8 cut(s) 98, 165, 176, 263, 278, 389, 987, 2678
HphI GGTGA 7 cut(s) 316, 1531, 1761, 2055, 2073, 2410, 2927
Hpy166II GTNNAC 8 cut(s) 350, 384, 541, 688, 2081, 2196, 2690, 3322
Hpy8I GTNNAC 8 cut(s) 350, 384, 541, 688, 2081, 2196, 2690, 3322
Hpy99I CGWCG 1 cut(s) 139
HpyCH4III ACNGT 8 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155, 3171
HpyCH4IV ACGT 7 cut(s) 122, 137, 362, 1230, 2019, 2839, 2879
HpyF10VI GCNNNNNNNGC 8 cut(s) 176, 179, 614, 677, 1462, 1963, 2648, 2889
HpyF3I CTNAG 7 cut(s) 59, 251, 1073, 1618, 1660, 2094, 3105
HpySE526I ACGT 7 cut(s) 122, 137, 362, 1230, 2019, 2839, 2879
HspAI GCGC 1 cut(s) 77
Kpn2I TCCGGA 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 975
KspAI GTTAAC 2 cut(s) 384, 3322
LmnI GCTCC 7 cut(s) 235, 291, 602, 1438, 1600, 2888, 3223
LweI GCATC 6 cut(s) 406, 1465, 2235, 2974, 3150, 3298
MabI ACCWGGT 1 cut(s) 915
MaeI CTAG 6 cut(s) 11, 588, 672, 1224, 2412, 3149
MaeII ACGT 7 cut(s) 122, 137, 362, 1230, 2019, 2839, 2879
MfeI CAATTG 6 cut(s) 1174, 1187, 1193, 2355, 2540, 3264
MflI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
MhlI GDGCHC 2 cut(s) 1539, 2885
MlyI GAGTC 4 cut(s) 332, 584, 2315, 2891
MmeI TCCRAC 5 cut(s) 158, 438, 1244, 1353, 2991
Mph1103I ATGCAT 2 cut(s) 1215, 3165
MroI TCCGGA 1 cut(s) 388
MseI TTAA 5 cut(s) 231, 383, 798, 1731, 3321
MslI CAYNNNNRTG 6 cut(s) 263, 1290, 1418, 2260, 2946, 3071
MspA1I CMGCKG 2 cut(s) 422, 2171
MspI CCGG 8 cut(s) 98, 165, 176, 263, 278, 389, 987, 2678
MspR9I CCNGG 6 cut(s) 279, 917, 1575, 2679, 3122, 3209
MunI CAATTG 6 cut(s) 1174, 1187, 1193, 2355, 2540, 3264
Mva1269I GAATGC 3 cut(s) 258, 546, 1149
MvaI CCWGG 4 cut(s) 917, 1575, 3122, 3209
MwoI GCNNNNNNNGC 8 cut(s) 176, 179, 614, 677, 1462, 1963, 2648, 2889
NciI CCSGG 2 cut(s) 279, 2679
NcoI CCATGG 5 cut(s) 964, 1011, 1350, 1710, 3041
NheI GCTAGC 1 cut(s) 2411
NlaIV GGNNCC 4 cut(s) 1393, 1511, 1624, 3219
NmeAIII GCCGAG 1 cut(s) 1701
NmuCI GTSAC 9 cut(s) 647, 779, 1336, 1406, 1420, 1519, 1767, 2039, 3001
NsiI ATGCAT 2 cut(s) 1215, 3165
NspI RCATGY 5 cut(s) 242, 797, 1503, 2259, 2667
NspV TTCGAA 1 cut(s) 104
OliI CACNNNNGTG 1 cut(s) 3071
PaeI GCATGC 1 cut(s) 2667
PaeR7I CTCGAG 1 cut(s) 1138
PaqCI CACCTGC 1 cut(s) 292
PceI AGGCCT 1 cut(s) 1860
PciI ACATGT 1 cut(s) 793
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 143
PctI GAATGC 3 cut(s) 258, 546, 1149
PfeI GAWTC 9 cut(s) 449, 935, 1085, 1247, 1647, 1834, 2057, 2319, 3053
Pfl23II CGTACG 1 cut(s) 359
PflMI CCANNNNNTGG 2 cut(s) 1442, 1781
PleI GAGTC 4 cut(s) 331, 583, 2314, 2891
PpsI GAGTC 4 cut(s) 331, 583, 2314, 2891
Ppu21I YACGTR 2 cut(s) 2020, 2880
PpuMI RGGWCCY 1 cut(s) 1622
PscI ACATGT 1 cut(s) 793
PsiI TTATAA 3 cut(s) 906, 2615, 2796
Psp5II RGGWCCY 1 cut(s) 1622
Psp6I CCWGG 4 cut(s) 915, 1573, 3120, 3207
PspEI GGTNACC 1 cut(s) 1767
PspGI CCWGG 4 cut(s) 915, 1573, 3120, 3207
PspLI CGTACG 1 cut(s) 359
PspN4I GGNNCC 4 cut(s) 1393, 1511, 1624, 3219
PspPI GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
PspPPI RGGWCCY 1 cut(s) 1622
PstI CTGCAG 4 cut(s) 682, 768, 841, 2170
PstNI CAGNNNCTG 1 cut(s) 3345
PsuI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
PvuII CAGCTG 2 cut(s) 422, 2171
RsaI GTAC 8 cut(s) 203, 243, 361, 513, 569, 985, 1319, 3173
RsaNI GTAC 8 cut(s) 202, 242, 360, 512, 568, 984, 1318, 3172
RseI CAYNNNNRTG 6 cut(s) 263, 1290, 1418, 2260, 2946, 3071
SalI GTCGAC 1 cut(s) 686
SaqAI TTAA 5 cut(s) 231, 383, 798, 1731, 3321
Sau96I GGNCC 8 cut(s) 347, 826, 860, 924, 1550, 1622, 2816, 2990
SchI GAGTC 4 cut(s) 332, 584, 2315, 2891
ScrFI CCNGG 6 cut(s) 279, 917, 1575, 2679, 3122, 3209
SduI GDGCHC 2 cut(s) 1539, 2885
SexAI ACCWGGT 1 cut(s) 915
SfaNI GCATC 6 cut(s) 406, 1465, 2235, 2974, 3150, 3298
SfcI CTRYAG 6 cut(s) 678, 764, 837, 1869, 2166, 3249
Sfr274I CTCGAG 1 cut(s) 1138
SfuI TTCGAA 1 cut(s) 104
SgrAI CRCCGGYG 1 cut(s) 175
SinI GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
SlaI CTCGAG 1 cut(s) 1138
SmiMI CAYNNNNRTG 6 cut(s) 263, 1290, 1418, 2260, 2946, 3071
SmlI CTYRAG 3 cut(s) 353, 1138, 1304
SmoI CTYRAG 3 cut(s) 353, 1138, 1304
SphI GCATGC 1 cut(s) 2667
SseBI AGGCCT 1 cut(s) 1860
SsiI CCGC 8 cut(s) 179, 255, 288, 320, 956, 1615, 1890, 2248
SspI AATATT 1 cut(s) 1134
SspMI CTAG 6 cut(s) 11, 588, 672, 1224, 2412, 3149
StuI AGGCCT 1 cut(s) 1860
StyD4I CCNGG 6 cut(s) 277, 915, 1573, 2677, 3120, 3207
StyI CCWWGG 8 cut(s) 964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
TaaI ACNGT 8 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155, 3171
TaiI ACGT 7 cut(s) 125, 140, 365, 1233, 2022, 2842, 2882
TatI WGTACW 3 cut(s) 241, 511, 1317
TauI GCSGC 3 cut(s) 182, 258, 2251
TfiI GAWTC 9 cut(s) 449, 935, 1085, 1247, 1647, 1834, 2057, 2319, 3053
Tru1I TTAA 5 cut(s) 231, 383, 798, 1731, 3321
Tru9I TTAA 5 cut(s) 231, 383, 798, 1731, 3321
TscAI CASTG 8 cut(s) 45, 548, 687, 768, 1345, 1782, 2149, 2247
TseFI GTSAC 9 cut(s) 647, 779, 1336, 1406, 1420, 1519, 1767, 2039, 3001
Tsp45I GTSAC 9 cut(s) 647, 779, 1336, 1406, 1420, 1519, 1767, 2039, 3001
TspGWI ACGGA 3 cut(s) 2149, 2820, 3041
TspRI CASTG 8 cut(s) 45, 548, 687, 768, 1345, 1782, 2149, 2247
Van91I CCANNNNNTGG 2 cut(s) 1442, 1781
VpaK11BI GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
XapI RAATTY 2 cut(s) 2860, 3230
XceI RCATGY 5 cut(s) 242, 797, 1503, 2259, 2667
XcmI CCANNNNNNNNNTGG 1 cut(s) 2945
XhoI CTCGAG 1 cut(s) 1138
XmiI GTMKAC 3 cut(s) 687, 2195, 2689
XspI CTAG 6 cut(s) 11, 588, 672, 1224, 2412, 3149
Zsp2I ATGCAT 2 cut(s) 1215, 3165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.