Rh2AG654400

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005447
Physical Location & Seq
Reverse (-)
87159732 .. 87168365
8634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG654400.1

Sequence Viewer

Length: 2619 bp
ATGGAGGTGCTAGGGTTCAAAGCGAAGTCCAAAATAGCACTGCTCGTTGTTCTCGTGCTGAGCTTTGGCTTCGTGGCGCTGTATGGCCTGTTGAAGCCGGTTTCGAACGGCTGTAACATGACGTATATGTATCCGACGTATATTCCGATACAGATGTCTAACTCCGGTGCTGCACCGGCGGCGAAGTATGGGCTGTATTTGTACCACGAAGGCTGGAAGACGATTGATTTTAAGGAGCATGTACAGAAGCTAAGCGGCATTCCGGTGCTTTTTATTCCGGGGAATGGCGGGAGCTATAAGCAGGTGAGGTCATTGGCTGCGGAATCTGATAGGGCGTATAAGGTAGGTCCACTTGAGCGTACGTTTTATGAAGAAGCTCGGTTAACTCCGGAAGAGGGAGGGGAGGAGGATATGGATGCAGCTGGCTTTGAGTTGAAGAACCAATACGATTCGAGGTTGGACTGGTTCGCTGTGGATCTTGAAGGAGAACACTCTGCTATGGATAGTGCTGTACTTGAACAACACACACAATATGTTGTCCGCTGCATTCACAGGATTTTGGATCAGTACGAGGAGTCTTTCAAGGCTAGAGAAAAGGAAGGAGCTGCAACCTCTGCGACTTTGCCAAAAAGTGTTATATTGGTTGGTCACTCTATGGGTGGTTTCGTTGCTAGAGCTGCAGTGGTCGACCCTCTTTTGAGAAAGTCAGCAGTTGAAACAATTATTACACTTTCCAGCCCACACCAACAACCTCCTGTGGCACTGCAGCCTTCCTACGGTCGCTACTTTAGACATGTTAATCGAGAATGGAGAAAGGGATATGAGGTCCAAACAACTGCAGCAGGACATTACTTGTCTGGTCCTGCTCTCTCTAATGTTGTTGTCATATCTATTTCTGGTGGTTATAATGATTACCAGGTAAGGACCAATTATGAATCCCTTGATGGCATTGTGCCGCCTACCCATGGTTTTATGATCAGTAGTACCGGCATGAAAAATGTTTGGTTATCCATGGAACATCAAGTTATCTTATGGTGCAATCAATTAGTTATCCAAGTATCACACACTCTCCTTAGTTTGGCAGATTCCAGAACAGGCCAGCCTTTCTCTGACACACGAATAAGACTTGCAATATTCTCGAGAATGCTTCGTAGTGCAATACCACAAAGTTTCAATTGGAGGAAGCAATTGCAATTGTCTCAACAGTCAATGCATGCTCCAACTAGAGACTTGAAAGACAGAACTGAATCACTATACATCCCTGCTGCTTGTCCAAGCAATGTTCATTGGAGTGATGATGGCCTTGAGAGGGACTTGTACATTCAGACAACCACCGTCACTGTTTTAGCCATGGATGGTCGAAGACGGTGGTTGGACATTCAAAAACTGGGATCCAATGGAAGAAGTCACTTCATGTTTGTGACCAACCTTGCTCCATGTTCTGGGGTTAGACTGCATCTGTGGCCTGACAAAAGAAACTCAACTTCAGACTTGCCTGCATGTATAAGGATCCATGAGGTGACATCGAAGATGGTGCTCATTCCATCAGGACCAGCACCAAGACAGATTGAACCAGGAAGCCAAACAGAGCAAGCTCCCCCTTCAGCTATACTCCGCTTAGGACCCAAGGATATGCGTGGTTTCAGATTCCTGACCATCTCAGTTGCACCACGTCCGAGTATTTCAGGCAGACCTCCACCAGCAGTTTCCATGGCAGTTGGTCAGTTTTTTAATCCAGCGGAAGGGGAGAGAGAGATCTCTCCTTGGTCACCTCCACTGTCTGGTTATTCTCACAAGGATTTATCTTGGAAGGAAGATCATCCCCTTGCTTTGAATCTATCATTCACAAGTAGTTTAGGCCTTCTGCCTGTAAAGTTCTCTTTGAAGACCGCAGGTTGTGGAATAAAGAGTTCTGGACTTCCAGATGAACAGGCTGGAGATATTGATAATAGCAAGCTCTGTAAGCTTCGCTGTTTCCCACCAGTTGCATTTGCTTGGGATGAAACAGCAGGTCTCCACGTATTCACAGATTTGTATAGTGACAAAATTGTTGTTGATTCATCACCATCGCTTTGGAGTTCACCCAGAAGTTCTGAGAAAACCTCTGTTATGTTGGTGGTAGATCCACATTGTTCATATAGCAGTGCGATGACTGTTTCCGTAACTGCAGTTGCCAGTAGGTTTTTGCTTGTGTATACTTCACAGATTATTGGTTTCTCCATTGTTGTTATCTTTTTTGCACTGATGCGGCAAGCATGTGCATGGGATCGTAACCTACCCATACCCTCGATGTTGAAGGCTCTGGAGTCTAATTTGAGATTCCCAATTCCATTTATCTACCTTGCTATTGCACCAATTGGTCTTTCTTTACTCGTTGGTTTTTGGATTTCTCAACCATCTCCTTCATTTGCTAGCTTCACCATTGTATCAGTTGTCTGCTATTTCCTTGCTAATGGCTTCATAGCCATTCTGATTTTGACATCCCAGTTCGCCTTTTATGGGGTTGCTCTGCTACATATTTTCATCAAGAAAAGGTTTCAATTGGGGGGGAAAACTTCTCAATGGTTTGTGAATATATCCTCTGGTTTCTGTTCATGGAAGGTAAACTTTATCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

872

Amino Acids

96.73

Weight (kDa)

8.8

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGAP1 PF07819 84 - 359 3.2e-82 PGAP1-like alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 906
AarI CACCTGC 1 cut(s) 292
Acc36I ACCTGC 3 cut(s) 292, 1883, 2000
AccB7I CCANNNNNTGG 2 cut(s) 1442, 1781
AccI GTMKAC 2 cut(s) 687, 2195
AccIII TCCGGA 1 cut(s) 388
AclWI GGATC 8 cut(s) 483, 570, 1386, 1399, 1504, 1517, 2117, 2274
AcuI CTGAAG 2 cut(s) 1470, 1587
AfaI GTAC 7 cut(s) 203, 243, 361, 513, 569, 985, 1319
AflIII ACRYGT 1 cut(s) 793
AjiI CACGTC 1 cut(s) 1673
AjnI CCWGG 2 cut(s) 915, 1573
AjuI GAANNNNNNNTTGG 2 cut(s) 2347, 2379
Alw21I GWGCWC 1 cut(s) 1539
Alw26I GTCTC 3 cut(s) 1203, 1221, 2018
AlwI GGATC 8 cut(s) 483, 570, 1386, 1399, 1504, 1517, 2117, 2274
Ama87I CYCGRG 1 cut(s) 1138
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 5 cut(s) 85, 1096, 1300, 1463, 1858
ApeKI GCWGC 9 cut(s) 170, 317, 419, 543, 605, 677, 766, 839, 1265
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 6 cut(s) 316, 1531, 1761, 2055, 2073, 2410
AsuII TTCGAA 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 2411
AvaI CYCGRG 1 cut(s) 1138
AvaII GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
BaeI ACNNNNGTAYC 4 cut(s) 140, 173, 2409, 2442
BamHI GGATCC 2 cut(s) 1391, 1509
BarI GAAGNNNNNNTAC 2 cut(s) 428, 460
BauI CACGAG 1 cut(s) 53
BbsI GAAGAC 3 cut(s) 224, 1369, 1892
Bbv12I GWGCWC 1 cut(s) 1539
BbvI GCAGC 9 cut(s) 157, 304, 431, 530, 592, 664, 778, 851, 1252
BccI CCATC 8 cut(s) 938, 1292, 1349, 1525, 1552, 1664, 2074, 2404
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 4 cut(s) 1119, 1153, 1516, 1550
BciT130I CCWGG 2 cut(s) 917, 1575
BciVI GTATCC 1 cut(s) 141
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 3 cut(s) 1203, 1221, 2018
BfaI CTAG 5 cut(s) 11, 588, 672, 1224, 2412
BfmI CTRYAG 4 cut(s) 678, 764, 837, 2166
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 3 cut(s) 292, 1883, 2000
BfuI GTATCC 1 cut(s) 141
BglII AGATCT 1 cut(s) 1755
BlpI GCTNAGC 2 cut(s) 59, 251
Bme1390I CCNGG 3 cut(s) 279, 917, 1575
Bme18I GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
BmeT110I CYCGRG 1 cut(s) 1138
BmgBI CACGTC 1 cut(s) 1673
BmgT120I GGNCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
BmiI GGNNCC 3 cut(s) 1393, 1511, 1624
BmrFI CCNGG 3 cut(s) 279, 917, 1575
BmrI ACTGGG 2 cut(s) 1397, 2479
BmsI GCATC 3 cut(s) 406, 1465, 2235
BmtI GCTAGC 1 cut(s) 2415
BmuI ACTGGG 2 cut(s) 1397, 2479
BpiI GAAGAC 3 cut(s) 224, 1369, 1892
BplI GAGNNNNNCTC 2 cut(s) 2087, 2119
BpmI CTGGAG 2 cut(s) 1956, 2324
Bpu10I CCTNAGC 1 cut(s) 1618
Bpu1102I GCTNAGC 2 cut(s) 59, 251
Bpu14I TTCGAA 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 374, 1325
BpuMI CCSGG 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 2020
BsaI GGTCTC 1 cut(s) 2018
BsaJI CCNNGG 7 cut(s) 278, 964, 1011, 1350, 1626, 1710, 1763
BsaWI WCCGGW 3 cut(s) 164, 262, 388
BsaXI ACNNNNNCTCC 2 cut(s) 1053, 1083
Bse118I RCCGGY 3 cut(s) 97, 175, 986
Bse1I ACTGG 5 cut(s) 467, 1392, 1982, 2175, 2485
Bse3DI GCAATG 1 cut(s) 1285
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 2 cut(s) 917, 1575
BseDI CCNNGG 7 cut(s) 278, 964, 1011, 1350, 1626, 1710, 1763
BseGI GGATG 6 cut(s) 421, 1257, 1360, 1819, 2005, 2480
BseMI GCAATG 1 cut(s) 1285
BseMII CTCAG 3 cut(s) 50, 1674, 2085
BseNI ACTGG 5 cut(s) 467, 1392, 1982, 2175, 2485
BseRI GAGGAG 2 cut(s) 419, 587
BseXI GCAGC 9 cut(s) 157, 304, 431, 530, 592, 664, 778, 851, 1252
BsgI GTGCAG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 781
BshFI GGCC 5 cut(s) 87, 1098, 1302, 1465, 1860
BsiEI CGRYCG 1 cut(s) 781
BsiHKAI GWGCWC 1 cut(s) 1539
BsiHKCI CYCGRG 1 cut(s) 1138
BsiSI CCGG 7 cut(s) 98, 165, 176, 263, 278, 389, 987
BsiWI CGTACG 1 cut(s) 359
BslFI GGGAC 1 cut(s) 1325
BsmAI GTCTC 3 cut(s) 1203, 1221, 2018
BsmFI GGGAC 1 cut(s) 1325
BsmI GAATGC 3 cut(s) 258, 546, 1149
BsnI GGCC 5 cut(s) 87, 1098, 1302, 1465, 1860
Bso31I GGTCTC 1 cut(s) 2018
BsoBI CYCGRG 1 cut(s) 1138
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 1539
Bsp13I TCCGGA 1 cut(s) 388
Bsp1407I TGTACA 2 cut(s) 241, 1317
Bsp143I GATC 9 cut(s) 475, 562, 975, 1391, 1509, 1755, 1816, 2122, 2266
Bsp1720I GCTNAGC 2 cut(s) 59, 251
Bsp19I CCATGG 4 cut(s) 964, 1011, 1350, 1710
BspANI GGCC 5 cut(s) 87, 1098, 1302, 1465, 1860
BspCNI CTCAG 3 cut(s) 51, 1673, 2086
BspEI TCCGGA 1 cut(s) 388
BspLI GGNNCC 3 cut(s) 1393, 1511, 1624
BspMAI CTGCAG 4 cut(s) 682, 768, 841, 2170
BspMI ACCTGC 3 cut(s) 292, 1883, 2000
BspOI GCTAGC 1 cut(s) 2415
BspPI GGATC 8 cut(s) 483, 570, 1386, 1399, 1504, 1517, 2117, 2274
BspT104I TTCGAA 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 2018
BsrDI GCAATG 1 cut(s) 1285
BsrFI RCCGGY 3 cut(s) 97, 175, 986
BsrGI TGTACA 2 cut(s) 241, 1317
BsrI ACTGG 5 cut(s) 467, 1392, 1982, 2175, 2485
BssAI RCCGGY 3 cut(s) 97, 175, 986
BssECI CCNNGG 7 cut(s) 278, 964, 1011, 1350, 1626, 1710, 1763
BssMI GATC 9 cut(s) 475, 562, 975, 1391, 1509, 1755, 1816, 2122, 2266
BssNAI GTATAC 1 cut(s) 2196
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 6 cut(s) 964, 1011, 1350, 1626, 1710, 1763
Bst1107I GTATAC 1 cut(s) 2196
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 917, 1575
Bst4CI ACNGT 7 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155
Bst6I CTCTTC 1 cut(s) 387
BstAPI GCANNNNNTGC 1 cut(s) 614
BstAUI TGTACA 2 cut(s) 241, 1317
BstBAI YACGTR 1 cut(s) 2020
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 8 cut(s) 424, 1100, 1215, 1497, 1593, 1955, 2253, 2413
BstDEI CTNAG 6 cut(s) 59, 251, 1073, 1618, 1660, 2094
BstDSI CCRYGG 4 cut(s) 964, 1011, 1350, 1710
BstEII GGTNACC 1 cut(s) 1767
BstF5I GGATG 6 cut(s) 421, 1257, 1360, 1819, 2005, 2480
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 9 cut(s) 478, 565, 978, 1394, 1512, 1758, 1819, 2125, 2269
BstMAI GTCTC 3 cut(s) 1203, 1221, 2018
BstMBI GATC 9 cut(s) 475, 562, 975, 1391, 1509, 1755, 1816, 2122, 2266
BstMCI CGRYCG 1 cut(s) 781
BstMWI GCNNNNNNNGC 6 cut(s) 176, 179, 614, 677, 1462, 1963
BstNI CCWGG 2 cut(s) 917, 1575
BstNSI RCATGY 5 cut(s) 242, 797, 1217, 1503, 2259
BstPI GGTNACC 1 cut(s) 1767
BstSCI CCNGG 3 cut(s) 277, 915, 1573
BstSFI CTRYAG 4 cut(s) 678, 764, 837, 2166
BstV1I GCAGC 9 cut(s) 157, 304, 431, 530, 592, 664, 778, 851, 1252
BstV2I GAAGAC 3 cut(s) 224, 1369, 1892
BstX2I RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
BstXI CCANNNNNNTGG 1 cut(s) 2073
BstYI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
BstZ17I GTATAC 1 cut(s) 2196
BsuI GTATCC 1 cut(s) 141
BsuRI GGCC 5 cut(s) 87, 1098, 1302, 1465, 1860
BtgI CCRYGG 4 cut(s) 964, 1011, 1350, 1710
BtgZI GCGATG 2 cut(s) 2052, 2162
BtrI CACGTC 1 cut(s) 1673
BtsCI GGATG 6 cut(s) 421, 1257, 1360, 1819, 2005, 2480
BtsI GCAGTG 4 cut(s) 38, 687, 761, 2149
BtsIMutI CAGTG 7 cut(s) 38, 687, 761, 1338, 1775, 2149, 2240
BveI ACCTGC 3 cut(s) 292, 1883, 2000
Cac8I GCNNGC 8 cut(s) 424, 1100, 1215, 1497, 1593, 1955, 2253, 2413
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 3 cut(s) 97, 175, 986
Cfr13I GGNCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
CsiI ACCWGGT 1 cut(s) 915
Csp6I GTAC 7 cut(s) 202, 242, 360, 512, 568, 984, 1318
CviQI GTAC 7 cut(s) 202, 242, 360, 512, 568, 984, 1318
DdeI CTNAG 6 cut(s) 59, 251, 1073, 1618, 1660, 2094
DpnI GATC 9 cut(s) 477, 564, 977, 1393, 1511, 1757, 1818, 2124, 2268
DpnII GATC 9 cut(s) 475, 562, 975, 1391, 1509, 1755, 1816, 2122, 2266
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Eco130I CCWWGG 6 cut(s) 964, 1011, 1350, 1626, 1710, 1763
Eco147I AGGCCT 1 cut(s) 1860
Eco31I GGTCTC 1 cut(s) 2018
Eco47I GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
Eco57I CTGAAG 2 cut(s) 1470, 1587
Eco88I CYCGRG 1 cut(s) 1138
Eco91I GGTNACC 1 cut(s) 1767
EcoO109I RGGNCCY 1 cut(s) 1622
EcoO65I GGTNACC 1 cut(s) 1767
EcoRII CCWGG 2 cut(s) 915, 1573
EcoT14I CCWWGG 6 cut(s) 964, 1011, 1350, 1626, 1710, 1763
EcoT22I ATGCAT 1 cut(s) 1215
ErhI CCWWGG 6 cut(s) 964, 1011, 1350, 1626, 1710, 1763
FalI AAGNNNNNCTT 1 cut(s) 2591
FaqI GGGAC 1 cut(s) 1325
FauI CCCGC 1 cut(s) 281
FbaI TGATCA 1 cut(s) 975
FblI GTMKAC 2 cut(s) 687, 2195
FokI GGATG 6 cut(s) 428, 1244, 1367, 1806, 2012, 2467
FspBI CTAG 5 cut(s) 11, 588, 672, 1224, 2412
GlaI GCGC 1 cut(s) 78
GsuI CTGGAG 2 cut(s) 1956, 2324
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 5 cut(s) 87, 1098, 1302, 1465, 1860
HapII CCGG 7 cut(s) 98, 165, 176, 263, 278, 389, 987
HhaI GCGC 1 cut(s) 79
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 2 cut(s) 384, 688
HindII GTYRAC 2 cut(s) 384, 688
HindIII AAGCTT 1 cut(s) 1964
HpaI GTTAAC 1 cut(s) 384
HpaII CCGG 7 cut(s) 98, 165, 176, 263, 278, 389, 987
HphI GGTGA 6 cut(s) 316, 1531, 1761, 2055, 2073, 2410
Hpy166II GTNNAC 6 cut(s) 350, 384, 688, 2081, 2196, 2605
Hpy8I GTNNAC 6 cut(s) 350, 384, 688, 2081, 2196, 2605
Hpy99I CGWCG 1 cut(s) 139
HpyCH4III ACNGT 7 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155
HpyCH4IV ACGT 5 cut(s) 122, 137, 362, 1672, 2019
HpyF10VI GCNNNNNNNGC 6 cut(s) 176, 179, 614, 677, 1462, 1963
HpyF3I CTNAG 6 cut(s) 59, 251, 1073, 1618, 1660, 2094
HpySE526I ACGT 5 cut(s) 122, 137, 362, 1672, 2019
HspAI GCGC 1 cut(s) 77
Kpn2I TCCGGA 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 975
KspAI GTTAAC 1 cut(s) 384
Kzo9I GATC 9 cut(s) 475, 562, 975, 1391, 1509, 1755, 1816, 2122, 2266
LmnI GCTCC 6 cut(s) 235, 291, 602, 1222, 1438, 1600
Lsp1109I GCAGC 9 cut(s) 157, 304, 431, 530, 592, 664, 778, 851, 1252
LweI GCATC 3 cut(s) 406, 1465, 2235
MabI ACCWGGT 1 cut(s) 915
MaeI CTAG 5 cut(s) 11, 588, 672, 1224, 2412
MaeII ACGT 5 cut(s) 122, 137, 362, 1672, 2019
MalI GATC 9 cut(s) 477, 564, 977, 1393, 1511, 1757, 1818, 2124, 2268
MboI GATC 9 cut(s) 475, 562, 975, 1391, 1509, 1755, 1816, 2122, 2266
MboII GAAGA 9 cut(s) 229, 383, 404, 448, 1374, 1413, 1540, 1826, 1897
MfeI CAATTG 5 cut(s) 1174, 1187, 1193, 2355, 2540
MflI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
MhlI GDGCHC 1 cut(s) 1539
MlyI GAGTC 2 cut(s) 584, 2315
MmeI TCCRAC 4 cut(s) 158, 438, 1244, 1353
Mph1103I ATGCAT 1 cut(s) 1215
MroI TCCGGA 1 cut(s) 388
MseI TTAA 4 cut(s) 231, 383, 798, 1731
MslI CAYNNNNRTG 4 cut(s) 263, 1290, 1418, 2260
MspA1I CMGCKG 3 cut(s) 422, 543, 1739
MspI CCGG 7 cut(s) 98, 165, 176, 263, 278, 389, 987
MspR9I CCNGG 3 cut(s) 279, 917, 1575
MunI CAATTG 5 cut(s) 1174, 1187, 1193, 2355, 2540
Mva1269I GAATGC 3 cut(s) 258, 546, 1149
MvaI CCWGG 2 cut(s) 917, 1575
MwoI GCNNNNNNNGC 6 cut(s) 176, 179, 614, 677, 1462, 1963
NciI CCSGG 1 cut(s) 279
NcoI CCATGG 4 cut(s) 964, 1011, 1350, 1710
NdeII GATC 9 cut(s) 475, 562, 975, 1391, 1509, 1755, 1816, 2122, 2266
NheI GCTAGC 1 cut(s) 2411
NlaIV GGNNCC 3 cut(s) 1393, 1511, 1624
NmuCI GTSAC 7 cut(s) 647, 1336, 1406, 1420, 1519, 1767, 2039
NsiI ATGCAT 1 cut(s) 1215
NspI RCATGY 5 cut(s) 242, 797, 1217, 1503, 2259
NspV TTCGAA 1 cut(s) 104
PaeI GCATGC 1 cut(s) 1217
PaeR7I CTCGAG 1 cut(s) 1138
PaqCI CACCTGC 1 cut(s) 292
PceI AGGCCT 1 cut(s) 1860
PciI ACATGT 1 cut(s) 793
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 143
PctI GAATGC 3 cut(s) 258, 546, 1149
PfeI GAWTC 9 cut(s) 323, 449, 935, 1085, 1247, 1647, 1834, 2057, 2319
Pfl23II CGTACG 1 cut(s) 359
PflMI CCANNNNNTGG 2 cut(s) 1442, 1781
PleI GAGTC 2 cut(s) 583, 2314
PpsI GAGTC 2 cut(s) 583, 2314
Ppu21I YACGTR 1 cut(s) 2020
PpuMI RGGWCCY 1 cut(s) 1622
PscI ACATGT 1 cut(s) 793
PsiI TTATAA 1 cut(s) 906
Psp5II RGGWCCY 1 cut(s) 1622
Psp6I CCWGG 2 cut(s) 915, 1573
PspEI GGTNACC 1 cut(s) 1767
PspGI CCWGG 2 cut(s) 915, 1573
PspLI CGTACG 1 cut(s) 359
PspN4I GGNNCC 3 cut(s) 1393, 1511, 1624
PspPI GGNCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
PspPPI RGGWCCY 1 cut(s) 1622
PstI CTGCAG 4 cut(s) 682, 768, 841, 2170
PsuI RGATCY 5 cut(s) 475, 1391, 1509, 1755, 2122
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 7 cut(s) 203, 243, 361, 513, 569, 985, 1319
RsaNI GTAC 7 cut(s) 202, 242, 360, 512, 568, 984, 1318
RseI CAYNNNNRTG 4 cut(s) 263, 1290, 1418, 2260
SalI GTCGAC 1 cut(s) 686
SaqAI TTAA 4 cut(s) 231, 383, 798, 1731
Sau3AI GATC 9 cut(s) 475, 562, 975, 1391, 1509, 1755, 1816, 2122, 2266
Sau96I GGNCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
SchI GAGTC 2 cut(s) 584, 2315
ScrFI CCNGG 3 cut(s) 279, 917, 1575
SduI GDGCHC 1 cut(s) 1539
SexAI ACCWGGT 1 cut(s) 915
SfaNI GCATC 3 cut(s) 406, 1465, 2235
SfcI CTRYAG 4 cut(s) 678, 764, 837, 2166
Sfr274I CTCGAG 1 cut(s) 1138
SfuI TTCGAA 1 cut(s) 104
SgrAI CRCCGGYG 1 cut(s) 175
SinI GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
SlaI CTCGAG 1 cut(s) 1138
SmiMI CAYNNNNRTG 4 cut(s) 263, 1290, 1418, 2260
SmlI CTYRAG 3 cut(s) 353, 1138, 1304
SmoI CTYRAG 3 cut(s) 353, 1138, 1304
SphI GCATGC 1 cut(s) 1217
SseBI AGGCCT 1 cut(s) 1860
SspI AATATT 1 cut(s) 1134
SspMI CTAG 5 cut(s) 11, 588, 672, 1224, 2412
StuI AGGCCT 1 cut(s) 1860
StyD4I CCNGG 3 cut(s) 277, 915, 1573
StyI CCWWGG 6 cut(s) 964, 1011, 1350, 1626, 1710, 1763
TaaI ACNGT 7 cut(s) 779, 1206, 1336, 1342, 1368, 1779, 2155
TaiI ACGT 5 cut(s) 125, 140, 365, 1675, 2022
TaqI TCGA 8 cut(s) 104, 452, 687, 802, 1139, 1360, 1526, 2288
TatI WGTACW 3 cut(s) 241, 511, 1317
TauI GCSGC 4 cut(s) 182, 258, 958, 2251
TfiI GAWTC 9 cut(s) 323, 449, 935, 1085, 1247, 1647, 1834, 2057, 2319
Tru1I TTAA 4 cut(s) 231, 383, 798, 1731
Tru9I TTAA 4 cut(s) 231, 383, 798, 1731
TscAI CASTG 7 cut(s) 45, 687, 768, 1345, 1782, 2149, 2247
TseFI GTSAC 7 cut(s) 647, 1336, 1406, 1420, 1519, 1767, 2039
TseI GCWGC 9 cut(s) 170, 317, 419, 543, 605, 677, 766, 839, 1265
Tsp45I GTSAC 7 cut(s) 647, 1336, 1406, 1420, 1519, 1767, 2039
TspGWI ACGGA 1 cut(s) 2149
TspRI CASTG 7 cut(s) 45, 687, 768, 1345, 1782, 2149, 2247
Van91I CCANNNNNTGG 2 cut(s) 1442, 1781
VpaK11BI GGWCC 6 cut(s) 347, 826, 860, 924, 1550, 1622
XceI RCATGY 5 cut(s) 242, 797, 1217, 1503, 2259
XhoI CTCGAG 1 cut(s) 1138
XmiI GTMKAC 2 cut(s) 687, 2195
XspI CTAG 5 cut(s) 11, 588, 672, 1224, 2412
Zsp2I ATGCAT 1 cut(s) 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.