RLG00000022248

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005447
Physical Location & Seq
Reverse (-)
81921754 .. 81929577
7824 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022248

Sequence Viewer

Length: 2910 bp
ATGGAGGTGCTAGGGTTTAAAGCGAAGTTCAAAATAGCGCTGCTCGTTGTTCTCGTGCTGAGCTTTGGCTTTGCTGCGCTCTATGGCTTGTTGAAGCCGGTTTCGAACGGCTGTAACATGACGTATATGTATCCGACGTATATTCCGATACAGATGACTAGCTCCGGTGCTGCGTCGGACAAGTATGGGCTGTATTTGTACCACGAAGGCTGGAAGACGATTGATTTTAAGGAGCATTTACAGAAGCTAAGCGGCATTCCGGTGCTTTTTATTCCGGGGAATGGCGGGAGCTATAAGCAGGTGAGGTCGTTGGCTGCGGAATCTGATAGGGCGTATAAAGTAGGTCCACTTGAGCGTACGTTTTATGAAGAAGCTCGGTTAACTCCGGAAGAGGGAGGGGAGGAGGATATGGATGCAGCTGGCTTTGAGTTGCAGAACCAATACGATTCGAGGTTGGACTGGTTCGCTGTGGATCTTGAAGGAGAACACTCTGCTATGGATAGTGCTGTACTTGAACAACACACACAATATGTTGTCCACTGCATTCACAGGATTTTGGATCAGTACAAGGAGTCTTTCAAGGCTAGAGAAAAGGAAGGAGCTGCAACCTCTGCGACTTTGCCAAAAAGTGTTATATTGGTTGGTCACTCTATGGGTGGTTTCGTTGCTAGAGCTGCAGTGGTCGACCCTCTTTTGAGAAAGTCAGCAGTTGAAACAATTATTACACTTTCCAGCCCACACCAACAACCTCCTGTGGCACTGCAGCCTTCCTACGGTCACTACTTTAGACATGTTAATCGAGAATGGAGAAAGGGATATGAGGTCCAAACAACTGCAGCAGGACATTACTTGTCTGGTCCTGCTCTCTCTAATGTTGTTGTCATATCTATTTCTGGTGGTTATAATGATTACCAGGTAAGGACCAAGTATGAATCCCTTGATGGCATTGTGCCGCCTACCCATGGTTTTATGATCAGTAGTACCGGCATGAAAAATGTTTGGTTATCCATGGAACATCAAGTTATCTTATGGTGCAATCAATTAGTTATCCAAGTATCACACACTCTCCTTAGTTTGGCAGATTCCAGAACAGGCCAGCCTTTCTCTGACACACGAATAAGACTTGCAATATTCTCGAGAATGCTTCGTAGTGCAATACCACAAAGTTTCAATTGGAGGAAGCAATCGCAATTGCCTCAACAGTCAATGCATGCTCCAACTAGAGACGTGAAAGACAGAACTGAATCACTATACATCCCTGCTGCTTGTCCTAGCAATGTTCATTGGAGTGATGATGGCCTTGAGAGGGACTTATACATTCAGACAACCACCGTCACTGTTTTAGCCATGGATGGTCGAAGACGGTGGTTGGACATTCAAAAACTGGGATCCAATGGAAGAAGTCACTTCATGTTTGTGACCAACCTTGCTCCATGTTCTGGGGTTAGACTGCATCTATGGCCTGACAAAAGAAACTCAACTTCAGACTTGCCTGCATGTATAAGGATCCATGAGGTGACATCGAAGATGGTGCTCATTCCATCAGGACCAGCACCAAGACAGATTGAACCGGGAAGCCAAACAGAGCAAGCTCCCCCTTCAGCTATACTCCGCTTAGGACCCAAGGATATGCGTGGTTTCAGATTCCTGACCATCTCAGTTGCACCACGTCCGAGTATTTCAGGCAGACCTCCACCAGCAGTTTCCATGGCAGTTGGTCAGTTTTTTAATCCAGCGGAAGGGGAGAGAGAGATCTCTCCTTGGTCACCTCCACTGTCTGGTTATTCTCACAAGGATTTATCTTGGAAGGAAGATCATCCCCTTGCTTTGAATCTATCATTCACAAGTAGTTTAGGCCTTCTGCCTGTAAAGTTCTCTTTGAAGACCGCAGGTTGTGGAATAAAGAGTTCTGGACTTCCAGATGAACAGGCTGGAGATATCGATAATAGCAAGCTCTGTAAGCTTCGCTGTTTCCCACCAGTTGCATTTGCTTGGGATGAAACAGCAGGTCTCCATGTATTCACAGATTTGTATAGTGACAAAATTGTTGTTGATTCATCACCATTGCTTTGGAGTTCACCCAGAAGTTCTGAGAAAACCTCTGTTATGTTGGTGGTAGATCCACATTGTTCATATAGCAGTGCGATGACTGTTTCCGTAACTGCAGTTGCCAGTAGGTTTTTGCTTGTGTATACTTCACAGATTATTGGTTTCTCCATTGTTGTTATCTTTTTTGCACTGATGCGGCAAGCATGTGCATGGGATCGTAACCTACCCATACCCTCGATGTTGAAGGCTCTGGATTTTTTGACAGCTTCATTTCGCAGCCATGCACGAAGACATGAATTATTTGATTATAAGAAAGACGGAAATGGTGGGCCTGACAAACTTACATTCAAATGTGATGGTATATCAAACCAGAATTTTTCTTCTGGAGATACGTGCTCCAATAGCCCAGACTCTCCAAAAAGTTTTGGTGAAACTCAATTAGAGATATTCCATCATCGGCATGGGTTGTTTATTTTGCATCTTGTTGCAACGCTCATGTTTGGCCCATCATTTGTGACTTGGTTGGAGAGAATTGGGACGGATCATAGCTTTCCATGGGTCTTGGATTCAGCTCTTTCCACGGGTGTGATCCTTCATGGTATCTTCACTTCAAACCCTCAGTTCAATTCCTTCCTGGTTTCTTTCCCTCCAATCCGAAACCTAGAAGTGAGGATGCATTTACTGTACCTGTTTGCTGGATACTATTCCTATCTCTCTTCCCTGGCCTTGGCTCCATATAGAGAATTTTATGTTATGGCTGCTGTAGGCTATACTTCAATTGGTCTGACCCTCTTACAGAGATGGAACAAGGAGAAGGGAGATGCCCATTTTGTTAACCGAAAGCACTCTCACAGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

970

Amino Acids

108.08

Weight (kDa)

8.47

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGAP1 PF07819 83 - 358 1.7e-82 PGAP1-like alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 903, 2358
AarI CACCTGC 1 cut(s) 289
Acc36I ACCTGC 3 cut(s) 289, 1880, 1997
AccB7I CCANNNNNTGG 2 cut(s) 1439, 1778
AccI GTMKAC 2 cut(s) 684, 2192
AccIII TCCGGA 1 cut(s) 385
AciI CCGC 8 cut(s) 252, 285, 317, 953, 1612, 1736, 1887, 2245
AcsI RAATTY 2 cut(s) 2422, 2792
AcuI CTGAAG 2 cut(s) 1467, 1584
AfaI GTAC 6 cut(s) 200, 358, 510, 566, 982, 2735
AfeI AGCGCT 1 cut(s) 39
AflIII ACRYGT 1 cut(s) 790
AjiI CACGTC 2 cut(s) 1228, 1670
AjnI CCWGG 3 cut(s) 912, 2682, 2769
AleI CACNNNNGTG 1 cut(s) 2633
Alw21I GWGCWC 2 cut(s) 1536, 2447
Alw26I GTCTC 2 cut(s) 1218, 2015
AlwNI CAGNNNCTG 1 cut(s) 2907
Ama87I CYCGRG 1 cut(s) 1135
Aor13HI TCCGGA 1 cut(s) 385
Aor51HI AGCGCT 1 cut(s) 39
AoxI GGCC 7 cut(s) 1093, 1297, 1460, 1855, 2378, 2551, 2772
ApoI RAATTY 2 cut(s) 2422, 2792
AspLEI GCGC 2 cut(s) 40, 79
AspS9I GGNCC 8 cut(s) 344, 823, 857, 921, 1547, 1619, 2378, 2552
AsuC2I CCSGG 2 cut(s) 276, 1572
AsuHPI GGTGA 6 cut(s) 313, 1528, 1758, 2052, 2070, 2489
AsuII TTCGAA 1 cut(s) 104
AvaI CYCGRG 1 cut(s) 1135
AvaII GGWCC 6 cut(s) 344, 823, 857, 921, 1547, 1619
BamHI GGATCC 2 cut(s) 1388, 1506
BarI GAAGNNNNNNTAC 2 cut(s) 2641, 2673
BauI CACGAG 1 cut(s) 53
BbsI GAAGAC 4 cut(s) 221, 1366, 1889, 2344
Bbv12I GWGCWC 2 cut(s) 1536, 2447
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 4 cut(s) 1116, 1150, 1513, 1547
BciT130I CCWGG 3 cut(s) 914, 2684, 2771
BciVI GTATCC 2 cut(s) 141, 2741
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 2 cut(s) 276, 1572
BcoDI GTCTC 2 cut(s) 1218, 2015
BfaI CTAG 7 cut(s) 11, 159, 585, 669, 1221, 1272, 2711
BfmI CTRYAG 5 cut(s) 675, 761, 834, 2163, 2811
BfoI RGCGCY 1 cut(s) 41
BfuAI ACCTGC 3 cut(s) 289, 1880, 1997
BfuI GTATCC 2 cut(s) 141, 2741
BglII AGATCT 1 cut(s) 1752
BlpI GCTNAGC 2 cut(s) 59, 248
Bme1390I CCNGG 5 cut(s) 276, 914, 1572, 2684, 2771
Bme18I GGWCC 6 cut(s) 344, 823, 857, 921, 1547, 1619
BmeT110I CYCGRG 1 cut(s) 1135
BmgBI CACGTC 2 cut(s) 1228, 1670
BmgT120I GGNCC 8 cut(s) 344, 823, 857, 921, 1547, 1619, 2378, 2552
BmiI GGNNCC 4 cut(s) 1390, 1508, 1621, 2781
BmrFI CCNGG 5 cut(s) 276, 914, 1572, 2684, 2771
BmrI ACTGGG 1 cut(s) 1394
BmsI GCATC 6 cut(s) 403, 1462, 2232, 2536, 2712, 2860
BmuI ACTGGG 1 cut(s) 1394
BpiI GAAGAC 4 cut(s) 221, 1366, 1889, 2344
BplI GAGNNNNNCTC 2 cut(s) 2084, 2116
BpmI CTGGAG 2 cut(s) 1953, 2454
Bpu10I CCTNAGC 1 cut(s) 1615
Bpu1102I GCTNAGC 2 cut(s) 59, 248
Bpu14I TTCGAA 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 371, 1322
BpuMI CCSGG 2 cut(s) 276, 1572
Bsa29I ATCGAT 1 cut(s) 1941
BsaAI YACGTR 1 cut(s) 2442
BsaI GGTCTC 1 cut(s) 2015
BsaWI WCCGGW 3 cut(s) 164, 259, 385
BsaXI ACNNNNNCTCC 2 cut(s) 1050, 1080
Bse118I RCCGGY 2 cut(s) 97, 983
Bse1I ACTGG 4 cut(s) 464, 1389, 1979, 2172
Bse3DI GCAATG 2 cut(s) 1282, 2063
BseAI TCCGGA 1 cut(s) 385
BseBI CCWGG 3 cut(s) 914, 2684, 2771
BseCI ATCGAT 1 cut(s) 1941
BseGI GGATG 6 cut(s) 418, 1254, 1357, 1816, 2002, 2727
BseMI GCAATG 2 cut(s) 1282, 2063
BseMII CTCAG 4 cut(s) 50, 1671, 2082, 2681
BseNI ACTGG 4 cut(s) 464, 1389, 1979, 2172
BseRI GAGGAG 1 cut(s) 416
BshFI GGCC 7 cut(s) 1095, 1299, 1462, 1857, 2380, 2553, 2774
BshVI ATCGAT 1 cut(s) 1941
BsiHKAI GWGCWC 2 cut(s) 1536, 2447
BsiHKCI CYCGRG 1 cut(s) 1135
BsiSI CCGG 7 cut(s) 98, 165, 260, 275, 386, 984, 1571
BsiWI CGTACG 1 cut(s) 356
BslFI GGGAC 2 cut(s) 1322, 2599
BsmAI GTCTC 2 cut(s) 1218, 2015
BsmBI CGTCTC 1 cut(s) 1218
BsmFI GGGAC 2 cut(s) 1322, 2599
BsmI GAATGC 3 cut(s) 255, 543, 1146
BsnI GGCC 7 cut(s) 1095, 1299, 1462, 1857, 2380, 2553, 2774
Bso31I GGTCTC 1 cut(s) 2015
BsoBI CYCGRG 1 cut(s) 1135
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 1536, 2447
Bsp13I TCCGGA 1 cut(s) 385
Bsp1720I GCTNAGC 2 cut(s) 59, 248
Bsp19I CCATGG 5 cut(s) 961, 1008, 1347, 1707, 2603
BspACI CCGC 8 cut(s) 252, 285, 317, 953, 1612, 1736, 1887, 2245
BspANI GGCC 7 cut(s) 1095, 1299, 1462, 1857, 2380, 2553, 2774
BspCNI CTCAG 4 cut(s) 51, 1670, 2083, 2680
BspDI ATCGAT 1 cut(s) 1941
BspEI TCCGGA 1 cut(s) 385
BspLI GGNNCC 4 cut(s) 1390, 1508, 1621, 2781
BspMAI CTGCAG 4 cut(s) 679, 765, 838, 2167
BspMI ACCTGC 3 cut(s) 289, 1880, 1997
BspT104I TTCGAA 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 2015
BsrDI GCAATG 2 cut(s) 1282, 2063
BsrFI RCCGGY 2 cut(s) 97, 983
BsrI ACTGG 4 cut(s) 464, 1389, 1979, 2172
BssAI RCCGGY 2 cut(s) 97, 983
BssNAI GTATAC 1 cut(s) 2193
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 8 cut(s) 961, 1008, 1347, 1623, 1707, 1760, 2603, 2775
Bst1107I GTATAC 1 cut(s) 2193
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 3 cut(s) 914, 2684, 2771
Bst4CI ACNGT 8 cut(s) 776, 1203, 1333, 1339, 1365, 1776, 2152, 2733
Bst6I CTCTTC 2 cut(s) 384, 2770
BstAPI GCANNNNNTGC 1 cut(s) 611
BstBAI YACGTR 1 cut(s) 2442
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 7 cut(s) 421, 1097, 1212, 1494, 1590, 1952, 2250
BstDEI CTNAG 7 cut(s) 59, 248, 1070, 1615, 1657, 2091, 2667
BstDSI CCRYGG 6 cut(s) 961, 1008, 1347, 1707, 2603, 2628
BstEII GGTNACC 1 cut(s) 1764
BstF5I GGATG 6 cut(s) 418, 1254, 1357, 1816, 2002, 2727
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 2 cut(s) 40, 79
BstMAI GTCTC 2 cut(s) 1218, 2015
BstMWI GCNNNNNNNGC 5 cut(s) 611, 674, 1459, 1960, 2451
BstNI CCWGG 3 cut(s) 914, 2684, 2771
BstNSI RCATGY 4 cut(s) 794, 1214, 1500, 2256
BstPI GGTNACC 1 cut(s) 1764
BstSCI CCNGG 5 cut(s) 274, 912, 1570, 2682, 2769
BstSFI CTRYAG 5 cut(s) 675, 761, 834, 2163, 2811
BstV2I GAAGAC 4 cut(s) 221, 1366, 1889, 2344
BstX2I RGATCY 5 cut(s) 472, 1388, 1506, 1752, 2119
BstXI CCANNNNNNTGG 1 cut(s) 2070
BstYI RGATCY 5 cut(s) 472, 1388, 1506, 1752, 2119
BstZ17I GTATAC 1 cut(s) 2193
Bsu15I ATCGAT 1 cut(s) 1941
BsuI GTATCC 2 cut(s) 141, 2741
BsuRI GGCC 7 cut(s) 1095, 1299, 1462, 1857, 2380, 2553, 2774
BsuTUI ATCGAT 1 cut(s) 1941
BtgI CCRYGG 6 cut(s) 961, 1008, 1347, 1707, 2603, 2628
BtgZI GCGATG 1 cut(s) 2159
BtrI CACGTC 2 cut(s) 1228, 1670
BtsCI GGATG 6 cut(s) 418, 1254, 1357, 1816, 2002, 2727
BtsI GCAGTG 4 cut(s) 538, 684, 758, 2146
BtsIMutI CAGTG 8 cut(s) 538, 684, 758, 1335, 1772, 2146, 2237, 2905
BveI ACCTGC 3 cut(s) 289, 1880, 1997
Cac8I GCNNGC 7 cut(s) 421, 1097, 1212, 1494, 1590, 1952, 2250
CaiI CAGNNNCTG 1 cut(s) 2907
CfoI GCGC 2 cut(s) 40, 79
Cfr10I RCCGGY 2 cut(s) 97, 983
Cfr13I GGNCC 8 cut(s) 344, 823, 857, 921, 1547, 1619, 2378, 2552
ClaI ATCGAT 1 cut(s) 1941
CseI GACGC 1 cut(s) 162
CsiI ACCWGGT 1 cut(s) 912
Csp6I GTAC 6 cut(s) 199, 357, 509, 565, 981, 2734
CviQI GTAC 6 cut(s) 199, 357, 509, 565, 981, 2734
DdeI CTNAG 7 cut(s) 59, 248, 1070, 1615, 1657, 2091, 2667
DraI TTTAAA 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 384, 2770
EarI CTCTTC 2 cut(s) 384, 2770
Eco130I CCWWGG 8 cut(s) 961, 1008, 1347, 1623, 1707, 1760, 2603, 2775
Eco147I AGGCCT 1 cut(s) 1857
Eco31I GGTCTC 1 cut(s) 2015
Eco32I GATATC 1 cut(s) 1939
Eco47I GGWCC 6 cut(s) 344, 823, 857, 921, 1547, 1619
Eco47III AGCGCT 1 cut(s) 39
Eco57I CTGAAG 2 cut(s) 1467, 1584
Eco88I CYCGRG 1 cut(s) 1135
Eco91I GGTNACC 1 cut(s) 1764
EcoO109I RGGNCCY 1 cut(s) 1619
EcoO65I GGTNACC 1 cut(s) 1764
EcoRII CCWGG 3 cut(s) 912, 2682, 2769
EcoRV GATATC 1 cut(s) 1939
EcoT14I CCWWGG 8 cut(s) 961, 1008, 1347, 1623, 1707, 1760, 2603, 2775
EcoT22I ATGCAT 2 cut(s) 1212, 2727
ErhI CCWWGG 8 cut(s) 961, 1008, 1347, 1623, 1707, 1760, 2603, 2775
Esp3I CGTCTC 1 cut(s) 1218
FaqI GGGAC 2 cut(s) 1322, 2599
FauI CCCGC 1 cut(s) 278
FbaI TGATCA 1 cut(s) 972
FblI GTMKAC 2 cut(s) 684, 2192
FokI GGATG 6 cut(s) 425, 1241, 1364, 1803, 2009, 2734
FspBI CTAG 7 cut(s) 11, 159, 585, 669, 1221, 1272, 2711
GlaI GCGC 2 cut(s) 39, 78
GsuI CTGGAG 2 cut(s) 1953, 2454
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 7 cut(s) 1095, 1299, 1462, 1857, 2380, 2553, 2774
HapII CCGG 7 cut(s) 98, 165, 260, 275, 386, 984, 1571
HgaI GACGC 1 cut(s) 162
HhaI GCGC 2 cut(s) 40, 79
Hin6I GCGC 2 cut(s) 38, 77
HinP1I GCGC 2 cut(s) 38, 77
HincII GTYRAC 3 cut(s) 381, 685, 2884
HindII GTYRAC 3 cut(s) 381, 685, 2884
HindIII AAGCTT 1 cut(s) 1961
HpaI GTTAAC 2 cut(s) 381, 2884
HpaII CCGG 7 cut(s) 98, 165, 260, 275, 386, 984, 1571
HphI GGTGA 6 cut(s) 313, 1528, 1758, 2052, 2070, 2489
Hpy166II GTNNAC 7 cut(s) 347, 381, 538, 685, 2078, 2193, 2884
Hpy8I GTNNAC 7 cut(s) 347, 381, 538, 685, 2078, 2193, 2884
Hpy99I CGWCG 2 cut(s) 139, 178
HpyCH4III ACNGT 8 cut(s) 776, 1203, 1333, 1339, 1365, 1776, 2152, 2733
HpyCH4IV ACGT 6 cut(s) 122, 137, 359, 1227, 1669, 2441
HpyF10VI GCNNNNNNNGC 5 cut(s) 611, 674, 1459, 1960, 2451
HpyF3I CTNAG 7 cut(s) 59, 248, 1070, 1615, 1657, 2091, 2667
HpySE526I ACGT 6 cut(s) 122, 137, 359, 1227, 1669, 2441
HspAI GCGC 2 cut(s) 38, 77
Kpn2I TCCGGA 1 cut(s) 385
Ksp22I TGATCA 1 cut(s) 972
KspAI GTTAAC 2 cut(s) 381, 2884
LmnI GCTCC 9 cut(s) 167, 232, 288, 599, 1219, 1435, 1597, 2450, 2785
LweI GCATC 6 cut(s) 403, 1462, 2232, 2536, 2712, 2860
MabI ACCWGGT 1 cut(s) 912
MaeI CTAG 7 cut(s) 11, 159, 585, 669, 1221, 1272, 2711
MaeII ACGT 6 cut(s) 122, 137, 359, 1227, 1669, 2441
MfeI CAATTG 3 cut(s) 1171, 1190, 2826
MflI RGATCY 5 cut(s) 472, 1388, 1506, 1752, 2119
MhlI GDGCHC 2 cut(s) 1536, 2447
MlyI GAGTC 2 cut(s) 581, 2453
MmeI TCCRAC 6 cut(s) 156, 158, 435, 1241, 1350, 2553
Mph1103I ATGCAT 2 cut(s) 1212, 2727
MroI TCCGGA 1 cut(s) 385
MseI TTAA 6 cut(s) 18, 228, 380, 795, 1728, 2883
MslI CAYNNNNRTG 7 cut(s) 260, 1287, 1415, 2257, 2398, 2508, 2633
MspA1I CMGCKG 2 cut(s) 419, 1736
MspI CCGG 7 cut(s) 98, 165, 260, 275, 386, 984, 1571
MspR9I CCNGG 5 cut(s) 276, 914, 1572, 2684, 2771
MunI CAATTG 3 cut(s) 1171, 1190, 2826
Mva1269I GAATGC 3 cut(s) 255, 543, 1146
MvaI CCWGG 3 cut(s) 914, 2684, 2771
MwoI GCNNNNNNNGC 5 cut(s) 611, 674, 1459, 1960, 2451
NciI CCSGG 2 cut(s) 276, 1572
NcoI CCATGG 5 cut(s) 961, 1008, 1347, 1707, 2603
NlaIV GGNNCC 4 cut(s) 1390, 1508, 1621, 2781
NmuCI GTSAC 9 cut(s) 644, 776, 1333, 1403, 1417, 1516, 1764, 2036, 2563
NsiI ATGCAT 2 cut(s) 1212, 2727
NspI RCATGY 4 cut(s) 794, 1214, 1500, 2256
NspV TTCGAA 1 cut(s) 104
OliI CACNNNNGTG 1 cut(s) 2633
PaeI GCATGC 1 cut(s) 1214
PaeR7I CTCGAG 1 cut(s) 1135
PaqCI CACCTGC 1 cut(s) 289
PceI AGGCCT 1 cut(s) 1857
PciI ACATGT 1 cut(s) 790
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 143
PctI GAATGC 3 cut(s) 255, 543, 1146
PfeI GAWTC 9 cut(s) 320, 446, 932, 1082, 1244, 1644, 1831, 2054, 2615
Pfl23II CGTACG 1 cut(s) 356
PflMI CCANNNNNTGG 2 cut(s) 1439, 1778
PleI GAGTC 2 cut(s) 580, 2453
PpsI GAGTC 2 cut(s) 580, 2453
Ppu21I YACGTR 1 cut(s) 2442
PpuMI RGGWCCY 1 cut(s) 1619
PscI ACATGT 1 cut(s) 790
PsiI TTATAA 2 cut(s) 903, 2358
Psp5II RGGWCCY 1 cut(s) 1619
Psp6I CCWGG 3 cut(s) 912, 2682, 2769
PspEI GGTNACC 1 cut(s) 1764
PspGI CCWGG 3 cut(s) 912, 2682, 2769
PspLI CGTACG 1 cut(s) 356
PspN4I GGNNCC 4 cut(s) 1390, 1508, 1621, 2781
PspPI GGNCC 8 cut(s) 344, 823, 857, 921, 1547, 1619, 2378, 2552
PspPPI RGGWCCY 1 cut(s) 1619
PstI CTGCAG 4 cut(s) 679, 765, 838, 2167
PstNI CAGNNNCTG 1 cut(s) 2907
PsuI RGATCY 5 cut(s) 472, 1388, 1506, 1752, 2119
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 6 cut(s) 200, 358, 510, 566, 982, 2735
RsaNI GTAC 6 cut(s) 199, 357, 509, 565, 981, 2734
RseI CAYNNNNRTG 7 cut(s) 260, 1287, 1415, 2257, 2398, 2508, 2633
SalI GTCGAC 1 cut(s) 683
SaqAI TTAA 6 cut(s) 18, 228, 380, 795, 1728, 2883
Sau96I GGNCC 8 cut(s) 344, 823, 857, 921, 1547, 1619, 2378, 2552
SchI GAGTC 2 cut(s) 581, 2453
ScrFI CCNGG 5 cut(s) 276, 914, 1572, 2684, 2771
SduI GDGCHC 2 cut(s) 1536, 2447
SexAI ACCWGGT 1 cut(s) 912
SfaNI GCATC 6 cut(s) 403, 1462, 2232, 2536, 2712, 2860
SfcI CTRYAG 5 cut(s) 675, 761, 834, 2163, 2811
Sfr274I CTCGAG 1 cut(s) 1135
SfuI TTCGAA 1 cut(s) 104
SinI GGWCC 6 cut(s) 344, 823, 857, 921, 1547, 1619
SlaI CTCGAG 1 cut(s) 1135
SmiMI CAYNNNNRTG 7 cut(s) 260, 1287, 1415, 2257, 2398, 2508, 2633
SmlI CTYRAG 3 cut(s) 350, 1135, 1301
SmoI CTYRAG 3 cut(s) 350, 1135, 1301
SphI GCATGC 1 cut(s) 1214
SseBI AGGCCT 1 cut(s) 1857
SsiI CCGC 8 cut(s) 252, 285, 317, 953, 1612, 1736, 1887, 2245
SspI AATATT 1 cut(s) 1131
SspMI CTAG 7 cut(s) 11, 159, 585, 669, 1221, 1272, 2711
StuI AGGCCT 1 cut(s) 1857
StyD4I CCNGG 5 cut(s) 274, 912, 1570, 2682, 2769
StyI CCWWGG 8 cut(s) 961, 1008, 1347, 1623, 1707, 1760, 2603, 2775
TaaI ACNGT 8 cut(s) 776, 1203, 1333, 1339, 1365, 1776, 2152, 2733
TaiI ACGT 6 cut(s) 125, 140, 362, 1230, 1672, 2444
TaqI TCGA 9 cut(s) 104, 449, 684, 799, 1136, 1357, 1523, 1941, 2285
TatI WGTACW 2 cut(s) 508, 564
TauI GCSGC 3 cut(s) 255, 955, 2248
TfiI GAWTC 9 cut(s) 320, 446, 932, 1082, 1244, 1644, 1831, 2054, 2615
Tru1I TTAA 6 cut(s) 18, 228, 380, 795, 1728, 2883
Tru9I TTAA 6 cut(s) 18, 228, 380, 795, 1728, 2883
TscAI CASTG 7 cut(s) 545, 684, 765, 1342, 1779, 2146, 2244
TseFI GTSAC 9 cut(s) 644, 776, 1333, 1403, 1417, 1516, 1764, 2036, 2563
Tsp45I GTSAC 9 cut(s) 644, 776, 1333, 1403, 1417, 1516, 1764, 2036, 2563
TspGWI ACGGA 3 cut(s) 2146, 2382, 2603
TspRI CASTG 7 cut(s) 545, 684, 765, 1342, 1779, 2146, 2244
Van91I CCANNNNNTGG 2 cut(s) 1439, 1778
VpaK11BI GGWCC 6 cut(s) 344, 823, 857, 921, 1547, 1619
XapI RAATTY 2 cut(s) 2422, 2792
XceI RCATGY 4 cut(s) 794, 1214, 1500, 2256
XcmI CCANNNNNNNNNTGG 1 cut(s) 2507
XhoI CTCGAG 1 cut(s) 1135
XmiI GTMKAC 2 cut(s) 684, 2192
XspI CTAG 7 cut(s) 11, 159, 585, 669, 1221, 1272, 2711
Zsp2I ATGCAT 2 cut(s) 1212, 2727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.