AT3G27520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014257
Physical Location & Seq
Forward (+)
10192294 .. 10193500
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27520.1

Sequence Viewer

Length: 597 bp
ATGGACTCACCGATGTCAACGAGTTCCATAGACGGAGACGAAGATTGGGAATGTTACGAAGACGATGGCTTCGTTTACAATCGAAAAAAGCGAACCCGTTTCGCCGACGCTGAAGAAACATCAAAACCGCCGGATCCGGAGTTAGATCGGGTGGTAGAAGAGAGGAATCGAAGGATACGGAAGAAGAGATTACTGGTGAAGCTCAAAAGAAAGTACCAGAGTGAGATCGACCAGTGGGAGATTTTATCGAATAGTTTCAATGCTATGCAAGAGAAAGCAGATCGATTTCAAACGGCGCAAAGAGAAGAGAGGTTAAATGCGAACGAAACGATGTCGTTTCCTGGGAATTCTTCCTCTACAACAGAAGGAGGCCGAGAATTTGGCGGAGAAGATGCTTCTAAGTCGCCGTCGTCTATGCTCGACCAACTTCTTTTTACGGTGGAAAGACAAGAAGCGGTAATCAACGAAGTCTCAAAGCTATGTGAAGTTACAGAGAACATATGTAGAGTGGAAGAAGAAGAAACGAAGCAGTCCTTCTTTGATCTTCCCATATGGAGCTCACCAACGGATCTAATGGCATCGCTTTGTGGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.85

Weight (kDa)

4.64

Isoelectric Point (pI)

67.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27520
fragaria_vesca FvH4_6g51790
malus_domestica MD09G1022900.v1.1 MD17G1022200.v1.1
prunus_persica Prupe.3G293900_v2.0.a1
pyrus_communis pycom111g01920
rosa_chinensis RchiOBHm_Chr2g0173291
rosa_laevigata RLG00000022177
rosa_multiflora Rmu_sc0010961.1_g000006 Rmu_sc0033776.1_g000004
rosa_roxburghii Rroxscaffold_2G00079070
rosa_rugosa Rorug02G0567100.1
rosa_samantha Rh2AG647500 Rh2BG658000 Rh2CG623400 Rh2DG671800
rosa_wichuraiana Rw2G053150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 3 cut(s) 128, 384, 455
AclWI GGATC 3 cut(s) 128, 141, 576
AcsI RAATTY 2 cut(s) 346, 377
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 1 cut(s) 215
AgsI TTSAA 2 cut(s) 259, 290
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 3 cut(s) 202, 478, 558
AluI AGCT 3 cut(s) 202, 478, 558
Alw21I GWGCWC 1 cut(s) 560
Alw26I GTCTC 2 cut(s) 30, 475
AlwI GGATC 3 cut(s) 128, 141, 576
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 1 cut(s) 370
ApoI RAATTY 2 cut(s) 346, 377
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
Asp700I GAANNNNTTC 1 cut(s) 254
AspLEI GCGC 1 cut(s) 298
AsuHPI GGTGA 2 cut(s) 208, 552
BamHI GGATCC 1 cut(s) 133
BanII GRGCYC 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 560
BccI CCATC 1 cut(s) 59
BceAI ACGGC 2 cut(s) 309, 391
BciT130I CCWGG 1 cut(s) 342
BciVI GTATCC 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 30, 475
BfuI GTATCC 1 cut(s) 168
Bme1390I CCNGG 1 cut(s) 342
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 2 cut(s) 382, 587
BpiI GAAGAC 1 cut(s) 66
Bsa29I ATCGAT 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 341
BsaWI WCCGGW 1 cut(s) 136
Bse1I ACTGG 2 cut(s) 198, 232
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 1 cut(s) 342
BseCI ATCGAT 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 341
BseNI ACTGG 2 cut(s) 198, 232
BshFI GGCC 1 cut(s) 372
BshVI ATCGAT 1 cut(s) 283
BsiHKAI GWGCWC 1 cut(s) 560
BsiSI CCGG 2 cut(s) 131, 137
BsmAI GTCTC 2 cut(s) 30, 475
BsmBI CGTCTC 1 cut(s) 30
BsnI GGCC 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 560
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 6 cut(s) 133, 145, 225, 280, 541, 568
BspACI CCGC 3 cut(s) 128, 384, 455
BspANI GGCC 1 cut(s) 372
BspDI ATCGAT 1 cut(s) 283
BspEI TCCGGA 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 3 cut(s) 128, 141, 576
BsrI ACTGG 2 cut(s) 198, 232
BssECI CCNNGG 1 cut(s) 341
BssMI GATC 6 cut(s) 133, 145, 225, 280, 541, 568
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 3 cut(s) 153, 179, 300
BstDEI CTNAG 1 cut(s) 399
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 6 cut(s) 136, 148, 228, 283, 544, 571
BstMAI GTCTC 2 cut(s) 30, 475
BstMBI GATC 6 cut(s) 133, 145, 225, 280, 541, 568
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 2 cut(s) 133, 568
BstYI RGATCY 2 cut(s) 133, 568
Bsu15I ATCGAT 1 cut(s) 283
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 1 cut(s) 372
BsuTUI ATCGAT 1 cut(s) 283
BtgZI GCGATG 1 cut(s) 564
BtsIMutI CAGTG 1 cut(s) 239
CfoI GCGC 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 283
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 214
CviJI RGCY 5 cut(s) 69, 202, 372, 478, 558
CviKI_1 RGCY 5 cut(s) 69, 202, 372, 478, 558
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 399
DpnI GATC 6 cut(s) 135, 147, 227, 282, 543, 570
DpnII GATC 6 cut(s) 133, 145, 225, 280, 541, 568
Eam1104I CTCTTC 3 cut(s) 153, 179, 300
EarI CTCTTC 3 cut(s) 153, 179, 300
EciI GGCGGA 1 cut(s) 399
Ecl136II GAGCTC 1 cut(s) 558
Eco24I GRGCYC 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 558
Eco57I CTGAAG 1 cut(s) 132
EcoICRI GAGCTC 1 cut(s) 558
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 560
Esp3I CGTCTC 1 cut(s) 30
FaiI YATR 8 cut(s) 29, 266, 416, 481, 500, 502, 551, 553
FalI AAGNNNNNCTT 2 cut(s) 518, 550
FauNDI CATATG 2 cut(s) 500, 551
FriOI GRGCYC 1 cut(s) 560
GlaI GCGC 1 cut(s) 297
HaeIII GGCC 1 cut(s) 372
HapII CCGG 2 cut(s) 131, 137
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 298
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HinfI GANTC 2 cut(s) 5, 166
HpaII CCGG 2 cut(s) 131, 137
HphI GGTGA 2 cut(s) 208, 552
Hpy166II GTNNAC 2 cut(s) 18, 76
Hpy188III TCNNGA 1 cut(s) 137
Hpy8I GTNNAC 2 cut(s) 18, 76
Hpy99I CGWCG 2 cut(s) 110, 412
HpyAV CCTTC 3 cut(s) 165, 359, 544
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4V TGCA 1 cut(s) 268
HpyF3I CTNAG 1 cut(s) 399
HspAI GCGC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 136
Kzo9I GATC 6 cut(s) 133, 145, 225, 280, 541, 568
LmnI GCTCC 1 cut(s) 555
LpnPI CCDG 7 cut(s) 144, 150, 179, 230, 245, 327, 354
LweI GCATC 2 cut(s) 382, 587
MaeIII GTNAC 2 cut(s) 53, 487
MalI GATC 6 cut(s) 135, 147, 227, 282, 543, 570
MboI GATC 6 cut(s) 133, 145, 225, 280, 541, 568
MflI RGATCY 2 cut(s) 133, 568
MhlI GDGCHC 1 cut(s) 560
MluCI AATT 2 cut(s) 346, 377
MnlI CCTC 4 cut(s) 156, 303, 362, 364
MroI TCCGGA 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 254
MseI TTAA 2 cut(s) 314, 595
MspI CCGG 2 cut(s) 131, 137
MspR9I CCNGG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 342
NdeI CATATG 2 cut(s) 500, 551
NdeII GATC 6 cut(s) 133, 145, 225, 280, 541, 568
NlaIV GGNNCC 1 cut(s) 135
NmeAIII GCCGAG 1 cut(s) 398
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 88
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 166
Psp124BI GAGCTC 1 cut(s) 560
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 1 cut(s) 135
PsuI RGATCY 2 cut(s) 133, 568
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 1 cut(s) 560
SaqAI TTAA 2 cut(s) 314, 595
Sau3AI GATC 6 cut(s) 133, 145, 225, 280, 541, 568
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 560
SetI ASST 4 cut(s) 204, 314, 480, 560
SfaNI GCATC 2 cut(s) 382, 587
Sse9I AATT 2 cut(s) 346, 377
SsiI CCGC 3 cut(s) 128, 384, 455
SstI GAGCTC 1 cut(s) 560
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 439
TaqI TCGA 6 cut(s) 82, 169, 228, 248, 283, 420
TasI AATT 2 cut(s) 346, 377
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 2 cut(s) 314, 595
Tru9I TTAA 2 cut(s) 314, 595
TscAI CASTG 1 cut(s) 239
TspGWI ACGGA 3 cut(s) 48, 193, 581
TspRI CASTG 1 cut(s) 239
XapI RAATTY 2 cut(s) 346, 377
XmnI GAANNNNTTC 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.