MD17G1022200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014257
Physical Location & Seq
Forward (+)
1643502 .. 1644911
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1022200.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGAGTCTGCAGCAGCTTGGGTGGACGCCCAAGAGTGGGAGGTCTGCAACGACGACGGCTTCGTCTTTAAGCGGAGGAAGAGGCGCCGGGTCGACCCAGATGCCGACTCGTCGGCTTCCGCTCCACCGTCCTCTTCCGCCGCGGATCTACGGGAAGATCCCGAGAAACTCCGGAGAGAGCGGAGGAGGAAGTTTCTTCTCAAGCTCAGAGATAAGTACCAGGCGGAGATCGACCAGTGGGAACTTTTGTCAAGCACGTTGCGCACAATGGAGGAGAGAGCGCGGCAGCTTCAACGGCAACGAGAGGCGCAGCAACAGCGAACGGAAGAGGAAGAGCGACAGAAAACGGCGTCGTCGGATGGGCCTAGTGCGTTAGAGATTCAGGGATCGGAGTTCGGTTGTGGGTCGTTGGTGGATGAGCTTCTCTTGCAGGTAGACGCACACGCAGCGATCATTCGTGATGTTTCAAGTCTCTGTGACACAGCGGACGCTCTATGCAACGAAGAAGAGGAACGGATTAGTCCGCTTTTCTTCGATATTCCGATCTGCAGTTCACCGAGGGAGCTCATGAGATCGTTGCAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.46

Weight (kDa)

4.84

Isoelectric Point (pI)

73.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27520
fragaria_vesca FvH4_6g51790
malus_domestica MD09G1022900.v1.1 MD17G1022200.v1.1
prunus_persica Prupe.3G293900_v2.0.a1
pyrus_communis pycom111g01920
rosa_chinensis RchiOBHm_Chr2g0173291
rosa_laevigata RLG00000022177
rosa_multiflora Rmu_sc0010961.1_g000006 Rmu_sc0033776.1_g000004
rosa_roxburghii Rroxscaffold_2G00079070
rosa_rugosa Rorug02G0567100.1
rosa_samantha Rh2AG647500 Rh2BG658000 Rh2CG623400 Rh2DG671800
rosa_wichuraiana Rw2G053150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
Acc16I TGCGCA 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 421
AccB1I GGYRCC 1 cut(s) 84
AccBSI CCGCTC 2 cut(s) 122, 181
AccI GTMKAC 2 cut(s) 93, 435
AccII CGCG 2 cut(s) 143, 283
AccIII TCCGGA 1 cut(s) 171
AclWI GGATC 3 cut(s) 152, 153, 394
AcyI GRCGYC 3 cut(s) 27, 85, 350
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 2 cut(s) 36, 37
AgsI TTSAA 2 cut(s) 293, 468
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 5 cut(s) 17, 205, 289, 421, 565
AluI AGCT 5 cut(s) 17, 205, 289, 421, 565
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 3 cut(s) 152, 153, 394
Ama87I CYCGRG 1 cut(s) 161
Aor13HI TCCGGA 1 cut(s) 171
AoxI GGCC 1 cut(s) 362
ApeKI GCWGC 5 cut(s) 11, 14, 286, 310, 446
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 4 cut(s) 87, 264, 283, 310
AspS9I GGNCC 1 cut(s) 362
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 546
AvaI CYCGRG 1 cut(s) 161
BanI GGYRCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 5 cut(s) 23, 26, 298, 322, 458
BccI CCATC 1 cut(s) 353
BceAI ACGGC 3 cut(s) 73, 311, 363
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 1 cut(s) 221
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 2 cut(s) 9, 547
BfoI RGCGCY 1 cut(s) 88
BfuAI ACCTGC 1 cut(s) 421
BisI GCNGC 7 cut(s) 12, 15, 141, 284, 287, 311, 447
BlsI GCNGC 7 cut(s) 13, 16, 142, 285, 288, 312, 448
Bme1390I CCNGG 2 cut(s) 89, 221
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 362
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 2 cut(s) 89, 221
BmsI GCATC 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 3 cut(s) 27, 85, 350
BsaJI CCNNGG 2 cut(s) 141, 557
BsaWI WCCGGW 1 cut(s) 171
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 37
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 587
BseAI TCCGGA 1 cut(s) 171
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 141, 557
BseGI GGATG 2 cut(s) 364, 421
BseLI CCNNNNNNNGG 2 cut(s) 36, 37
BseMI GCAATG 1 cut(s) 587
BseMII CTCAG 1 cut(s) 220
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 2 cut(s) 199, 287
BseXI GCAGC 5 cut(s) 23, 26, 298, 322, 458
Bsh1236I CGCG 2 cut(s) 143, 283
BshFI GGCC 1 cut(s) 364
BshNI GGYRCC 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 2 cut(s) 88, 172
BslI CCNNNNNNNGG 2 cut(s) 36, 37
BsmAI GTCTC 1 cut(s) 476
BsnI GGCC 1 cut(s) 364
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 567
Bsp13I TCCGGA 1 cut(s) 171
Bsp143I GATC 7 cut(s) 145, 157, 228, 386, 450, 543, 572
BspANI GGCC 1 cut(s) 364
BspCNI CTCAG 1 cut(s) 219
BspEI TCCGGA 1 cut(s) 171
BspFNI CGCG 2 cut(s) 143, 283
BspHI TCATGA 1 cut(s) 567
BspLI GGNNCC 1 cut(s) 86
BspMAI CTGCAG 2 cut(s) 13, 551
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 3 cut(s) 152, 153, 394
BspQI GCTCTTC 1 cut(s) 327
BspT107I GGYRCC 1 cut(s) 84
BsrBI CCGCTC 2 cut(s) 122, 181
BsrDI GCAATG 1 cut(s) 587
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 2 cut(s) 141, 557
BssMI GATC 7 cut(s) 145, 157, 228, 386, 450, 543, 572
BssNI GRCGYC 3 cut(s) 27, 85, 350
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 5 cut(s) 74, 139, 321, 327, 501
BstACI GRCGYC 3 cut(s) 27, 85, 350
BstDEI CTNAG 1 cut(s) 206
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 364, 421
BstFNI CGCG 2 cut(s) 143, 283
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 4 cut(s) 87, 264, 283, 310
BstKTI GATC 7 cut(s) 148, 160, 231, 389, 453, 546, 575
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 7 cut(s) 145, 157, 228, 386, 450, 543, 572
BstMWI GCNNNNNNNGC 5 cut(s) 261, 295, 316, 427, 446
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 87, 219
BstSFI CTRYAG 2 cut(s) 9, 547
BstUI CGCG 2 cut(s) 143, 283
BstV1I GCAGC 5 cut(s) 23, 26, 298, 322, 458
BstX2I RGATCY 2 cut(s) 145, 157
BstYI RGATCY 2 cut(s) 145, 157
BsuRI GGCC 1 cut(s) 364
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 2 cut(s) 364, 421
BtsIMutI CAGTG 1 cut(s) 242
BveI ACCTGC 1 cut(s) 421
CciI TCATGA 1 cut(s) 567
CfoI GCGC 4 cut(s) 87, 264, 283, 310
Cfr13I GGNCC 1 cut(s) 362
Cfr42I CCGCGG 1 cut(s) 144
CseI GACGC 4 cut(s) 35, 339, 446, 497
Csp6I GTAC 1 cut(s) 217
CviAII CATG 1 cut(s) 568
CviJI RGCY 8 cut(s) 17, 60, 116, 205, 289, 364, 421, 565
CviKI_1 RGCY 8 cut(s) 17, 60, 116, 205, 289, 364, 421, 565
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 1 cut(s) 206
DinI GGCGCC 1 cut(s) 86
DpnI GATC 7 cut(s) 147, 159, 230, 388, 452, 545, 574
DpnII GATC 7 cut(s) 145, 157, 228, 386, 450, 543, 572
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
Eam1104I CTCTTC 5 cut(s) 74, 139, 321, 327, 501
EarI CTCTTC 5 cut(s) 74, 139, 321, 327, 501
EciI GGCGGA 2 cut(s) 127, 239
Ecl136II GAGCTC 1 cut(s) 565
Eco24I GRGCYC 1 cut(s) 567
Eco53kI GAGCTC 1 cut(s) 565
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 567
EgeI GGCGCC 1 cut(s) 86
EheI GGCGCC 1 cut(s) 86
FaeI CATG 1 cut(s) 571
FaiI YATR 2 cut(s) 496, 569
FatI CATG 1 cut(s) 567
FblI GTMKAC 2 cut(s) 93, 435
Fnu4HI GCNGC 7 cut(s) 12, 15, 141, 284, 287, 311, 447
FokI GGATG 2 cut(s) 371, 428
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 7 cut(s) 12, 15, 141, 284, 287, 311, 447
FspBI CTAG 1 cut(s) 366
FspI TGCGCA 1 cut(s) 263
GlaI GCGC 4 cut(s) 86, 263, 282, 309
GluI GCNGC 7 cut(s) 12, 15, 141, 284, 287, 311, 447
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 1 cut(s) 364
HapII CCGG 2 cut(s) 88, 172
HgaI GACGC 4 cut(s) 35, 339, 446, 497
HhaI GCGC 4 cut(s) 87, 264, 283, 310
Hin1I GRCGYC 3 cut(s) 27, 85, 350
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 4 cut(s) 85, 262, 281, 308
HinP1I GCGC 4 cut(s) 85, 262, 281, 308
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 3 cut(s) 5, 107, 379
HpaII CCGG 2 cut(s) 88, 172
HphI GGTGA 1 cut(s) 546
Hpy166II GTNNAC 4 cut(s) 25, 94, 436, 554
Hpy188I TCNGA 4 cut(s) 209, 358, 391, 543
Hpy188III TCNNGA 4 cut(s) 161, 172, 458, 568
Hpy8I GTNNAC 4 cut(s) 25, 94, 436, 554
Hpy99I CGWCG 5 cut(s) 56, 59, 115, 355, 358
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 6 cut(s) 11, 48, 430, 498, 549, 580
HpyF10VI GCNNNNNNNGC 5 cut(s) 261, 295, 316, 427, 446
HpyF3I CTNAG 1 cut(s) 206
HpySE526I ACGT 1 cut(s) 257
Hsp92I GRCGYC 3 cut(s) 27, 85, 350
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 4 cut(s) 85, 262, 281, 308
KasI GGCGCC 1 cut(s) 84
Kpn2I TCCGGA 1 cut(s) 171
KspI CCGCGG 1 cut(s) 144
Kzo9I GATC 7 cut(s) 145, 157, 228, 386, 450, 543, 572
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 2 cut(s) 127, 562
LpnPI CCDG 8 cut(s) 101, 111, 185, 206, 233, 248, 368, 416
Lsp1109I GCAGC 5 cut(s) 23, 26, 298, 322, 458
LweI GCATC 1 cut(s) 91
MaeI CTAG 1 cut(s) 366
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 1 cut(s) 476
MalI GATC 7 cut(s) 147, 159, 230, 388, 452, 545, 574
MbiI CCGCTC 2 cut(s) 122, 181
MboI GATC 7 cut(s) 145, 157, 228, 386, 450, 543, 572
MboII GAAGA 9 cut(s) 91, 126, 167, 188, 338, 344, 515, 518, 523
MflI RGATCY 2 cut(s) 145, 157
MhlI GDGCHC 1 cut(s) 567
Mly113I GGCGCC 1 cut(s) 85
MlyI GAGTC 2 cut(s) 14, 101
MmeI TCCRAC 1 cut(s) 336
MroI TCCGGA 1 cut(s) 171
MseI TTAA 1 cut(s) 69
MspA1I CMGCKG 2 cut(s) 143, 485
MspI CCGG 2 cut(s) 88, 172
MspR9I CCNGG 2 cut(s) 89, 221
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 2 cut(s) 143, 283
MwoI GCNNNNNNNGC 5 cut(s) 261, 295, 316, 427, 446
NarI GGCGCC 1 cut(s) 85
NciI CCSGG 1 cut(s) 89
NdeII GATC 7 cut(s) 145, 157, 228, 386, 450, 543, 572
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 476
NsbI TGCGCA 1 cut(s) 263
PagI TCATGA 1 cut(s) 567
PciSI GCTCTTC 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 7 cut(s) 13, 16, 142, 285, 288, 312, 448
PleI GAGTC 2 cut(s) 13, 101
PluTI GGCGCC 1 cut(s) 88
PpsI GAGTC 2 cut(s) 13, 101
Psp124BI GAGCTC 1 cut(s) 567
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 86
PspPI GGNCC 1 cut(s) 362
PstI CTGCAG 2 cut(s) 13, 551
PsuI RGATCY 2 cut(s) 145, 157
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SacI GAGCTC 1 cut(s) 567
SacII CCGCGG 1 cut(s) 144
SalI GTCGAC 1 cut(s) 92
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 7 cut(s) 12, 15, 141, 284, 287, 311, 447
Sau3AI GATC 7 cut(s) 145, 157, 228, 386, 450, 543, 572
Sau96I GGNCC 1 cut(s) 362
SchI GAGTC 2 cut(s) 14, 101
ScrFI CCNGG 2 cut(s) 89, 221
SduI GDGCHC 1 cut(s) 567
SetI ASST 8 cut(s) 19, 45, 207, 260, 291, 423, 435, 567
SfaNI GCATC 1 cut(s) 91
SfcI CTRYAG 2 cut(s) 9, 547
SfoI GGCGCC 1 cut(s) 86
Sfr303I CCGCGG 1 cut(s) 144
SgrBI CCGCGG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
SspDI GGCGCC 1 cut(s) 84
SspMI CTAG 1 cut(s) 366
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 87, 219
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 1 cut(s) 260
TaqI TCGA 3 cut(s) 93, 231, 534
TauI GCSGC 2 cut(s) 143, 286
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 242
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 5 cut(s) 11, 14, 286, 310, 446
Tsp45I GTSAC 1 cut(s) 476
TspGWI ACGGA 2 cut(s) 338, 529
TspRI CASTG 1 cut(s) 242
XmiI GTMKAC 2 cut(s) 93, 435
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.