RLG00000022177

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014257
Physical Location & Seq
Reverse (-)
81548856 .. 81549715
860 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022177

Sequence Viewer

Length: 558 bp
ATGGAGTCAACGTCATCCGCGGCCTGGCAAAACGACGACGTATGGGAGCTCCGCCGCGACGACGACGGCTTCGTCTACAAGCGCAAGAAGAGGCGCCGCCTCGACGACGACCCCGCCCCACCGCCTCCGTCCTCCGCCGCAGATCTCCCGGACGAAACTGAGTCCCGCCGGAGAGAGTGGAGGGAGAAGGCTCTGCTGAAGCTGAAGACTCAGTACAAGGCCGAGATTGAGCACTGGGAGCTTTTATCGAACAGGCTGAAGGAAATGGAGGAGAAGACGAGTCAGATTCAGCGGCGGCGATTAGAGCAGCGAGAACGGGACCAAACGGCGTCGTTTAATGGTTCTGGCGTGGAGGGGAACCAGTGCTCGGAGTCGGTGCTGGATGAGCTGCTCTTGCAGGCCGAAGCTCAGGAATCGATCATTGCAGATGTTTCAAGTTTGTGTGACACGGTGGAGGCAATGTGTCGTACGGAAGAGGAAGATGTAGCTCAGTCTTTGATCAATCTTCCAATCTGGGGCTCACCACGCGAGCTCATGAGATCGCTTTGTGACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

21.47

Weight (kDa)

4.72

Isoelectric Point (pI)

69.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27520
fragaria_vesca FvH4_6g51790
malus_domestica MD09G1022900.v1.1 MD17G1022200.v1.1
prunus_persica Prupe.3G293900_v2.0.a1
pyrus_communis pycom111g01920
rosa_chinensis RchiOBHm_Chr2g0173291
rosa_laevigata RLG00000022177
rosa_multiflora Rmu_sc0010961.1_g000006 Rmu_sc0033776.1_g000004
rosa_roxburghii Rroxscaffold_2G00079070
rosa_rugosa Rorug02G0567100.1
rosa_samantha Rh2AG647500 Rh2BG658000 Rh2CG623400 Rh2DG671800
rosa_wichuraiana Rw2G053150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 75
AccII CGCG 3 cut(s) 20, 57, 528
AcuI CTGAAG 3 cut(s) 218, 224, 278
AcyI GRCGYC 2 cut(s) 94, 329
AfaI GTAC 2 cut(s) 215, 469
AfiI CCNNNNNNNGG 3 cut(s) 24, 367, 515
AgsI TTSAA 1 cut(s) 435
AjnI CCWGG 1 cut(s) 23
AluBI AGCT 7 cut(s) 49, 202, 241, 388, 407, 488, 532
AluI AGCT 7 cut(s) 49, 202, 241, 388, 407, 488, 532
Alw21I GWGCWC 4 cut(s) 51, 234, 368, 534
AoxI GGCC 3 cut(s) 21, 219, 399
ApeKI GCWGC 2 cut(s) 307, 388
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 2 cut(s) 84, 96
AspS9I GGNCC 1 cut(s) 319
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 513
AvaII GGWCC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 93
BanII GRGCYC 3 cut(s) 51, 521, 534
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BbsI GAAGAC 2 cut(s) 212, 281
Bbv12I GWGCWC 4 cut(s) 51, 234, 368, 534
BbvI GCAGC 2 cut(s) 319, 375
BceAI ACGGC 2 cut(s) 82, 342
BciT130I CCWGG 1 cut(s) 25
BclI TGATCA 1 cut(s) 498
BcnI CCSGG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 97
BglII AGATCT 1 cut(s) 142
BisI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
BlsI GCNGC 8 cut(s) 22, 56, 98, 139, 294, 297, 309, 390
Bme1390I CCNGG 2 cut(s) 25, 149
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 3 cut(s) 95, 320, 359
BmrFI CCNGG 2 cut(s) 25, 149
BmrI ACTGGG 1 cut(s) 244
BmuI ACTGGG 1 cut(s) 244
BpiI GAAGAC 2 cut(s) 212, 281
Bpu10I CCTNAGC 1 cut(s) 408
BpuMI CCSGG 1 cut(s) 149
Bsa29I ATCGAT 1 cut(s) 416
BsaHI GRCGYC 2 cut(s) 94, 329
BsaJI CCNNGG 1 cut(s) 18
BsaXI ACNNNNNCTCC 3 cut(s) 26, 446, 476
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 367, 515
Bse1I ACTGG 2 cut(s) 239, 361
Bse3DI GCAATG 2 cut(s) 420, 465
BseBI CCWGG 1 cut(s) 25
BseCI ATCGAT 1 cut(s) 416
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 2 cut(s) 14, 388
BseLI CCNNNNNNNGG 3 cut(s) 24, 367, 515
BseMI GCAATG 2 cut(s) 420, 465
BseMII CTCAG 4 cut(s) 150, 224, 422, 503
BseNI ACTGG 2 cut(s) 239, 361
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 2 cut(s) 319, 375
Bsh1236I CGCG 3 cut(s) 20, 57, 528
BshFI GGCC 3 cut(s) 23, 221, 401
BshNI GGYRCC 1 cut(s) 93
BshVI ATCGAT 1 cut(s) 416
BsiHKAI GWGCWC 4 cut(s) 51, 234, 368, 534
BsiSI CCGG 2 cut(s) 149, 169
BsiWI CGTACG 1 cut(s) 467
BslFI GGGAC 2 cut(s) 148, 332
BslI CCNNNNNNNGG 3 cut(s) 24, 367, 515
BsmFI GGGAC 2 cut(s) 148, 332
BsnI GGCC 3 cut(s) 23, 221, 401
Bsp1286I GDGCHC 5 cut(s) 51, 234, 368, 521, 534
Bsp143I GATC 4 cut(s) 142, 417, 498, 539
BspANI GGCC 3 cut(s) 23, 221, 401
BspCNI CTCAG 4 cut(s) 151, 223, 421, 502
BspDI ATCGAT 1 cut(s) 416
BspFNI CGCG 3 cut(s) 20, 57, 528
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 3 cut(s) 95, 320, 359
BspT107I GGYRCC 1 cut(s) 93
BsrDI GCAATG 2 cut(s) 420, 465
BsrI ACTGG 2 cut(s) 239, 361
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 4 cut(s) 142, 417, 498, 539
BssNI GRCGYC 2 cut(s) 94, 329
Bst2UI CCWGG 1 cut(s) 25
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 2 cut(s) 83, 468
BstACI GRCGYC 2 cut(s) 94, 329
BstC8I GCNNGC 2 cut(s) 399, 530
BstDEI CTNAG 4 cut(s) 159, 210, 408, 489
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 2 cut(s) 14, 388
BstFNI CGCG 3 cut(s) 20, 57, 528
BstH2I RGCGCY 1 cut(s) 97
BstHHI GCGC 2 cut(s) 84, 96
BstKTI GATC 4 cut(s) 145, 420, 501, 542
BstMBI GATC 4 cut(s) 142, 417, 498, 539
BstMWI GCNNNNNNNGC 5 cut(s) 238, 304, 385, 394, 525
BstNI CCWGG 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 23, 147
BstUI CGCG 3 cut(s) 20, 57, 528
BstV1I GCAGC 2 cut(s) 319, 375
BstV2I GAAGAC 2 cut(s) 212, 281
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
Bsu15I ATCGAT 1 cut(s) 416
BsuRI GGCC 3 cut(s) 23, 221, 401
BsuTUI ATCGAT 1 cut(s) 416
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 2 cut(s) 14, 388
BtsIMutI CAGTG 2 cut(s) 232, 368
Cac8I GCNNGC 2 cut(s) 399, 530
CciI TCATGA 1 cut(s) 534
CfoI GCGC 2 cut(s) 84, 96
Cfr13I GGNCC 1 cut(s) 319
Cfr42I CCGCGG 1 cut(s) 21
ClaI ATCGAT 1 cut(s) 416
CseI GACGC 1 cut(s) 318
Csp6I GTAC 2 cut(s) 214, 468
CviAII CATG 1 cut(s) 535
CviQI GTAC 2 cut(s) 214, 468
DdeI CTNAG 4 cut(s) 159, 210, 408, 489
DinI GGCGCC 1 cut(s) 95
DpnI GATC 4 cut(s) 144, 419, 500, 541
DpnII GATC 4 cut(s) 142, 417, 498, 539
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 83, 468
EarI CTCTTC 2 cut(s) 83, 468
EciI GGCGGA 2 cut(s) 41, 124
Ecl136II GAGCTC 2 cut(s) 49, 532
Eco24I GRGCYC 3 cut(s) 51, 521, 534
Eco47I GGWCC 1 cut(s) 319
Eco53kI GAGCTC 2 cut(s) 49, 532
Eco57I CTGAAG 3 cut(s) 218, 224, 278
EcoICRI GAGCTC 2 cut(s) 49, 532
EcoRII CCWGG 1 cut(s) 23
EcoT38I GRGCYC 3 cut(s) 51, 521, 534
EgeI GGCGCC 1 cut(s) 95
EheI GGCGCC 1 cut(s) 95
FaeI CATG 1 cut(s) 538
FaiI YATR 2 cut(s) 43, 536
FaqI GGGAC 2 cut(s) 148, 332
FatI CATG 1 cut(s) 534
FauI CCCGC 2 cut(s) 121, 173
FbaI TGATCA 1 cut(s) 498
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
FokI GGATG 1 cut(s) 395
FriOI GRGCYC 3 cut(s) 51, 521, 534
Fsp4HI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
GlaI GCGC 2 cut(s) 83, 95
GluI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
HaeII RGCGCY 1 cut(s) 97
HaeIII GGCC 3 cut(s) 23, 221, 401
HapII CCGG 2 cut(s) 149, 169
HgaI GACGC 1 cut(s) 318
HhaI GCGC 2 cut(s) 84, 96
Hin1I GRCGYC 2 cut(s) 94, 329
Hin1II CATG 1 cut(s) 538
Hin6I GCGC 2 cut(s) 82, 94
HinP1I GCGC 2 cut(s) 82, 94
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 7 cut(s) 5, 161, 208, 280, 286, 371, 413
HpaII CCGG 2 cut(s) 149, 169
HphI GGTGA 1 cut(s) 513
Hpy166II GTNNAC 2 cut(s) 9, 76
Hpy188I TCNGA 2 cut(s) 285, 370
Hpy188III TCNNGA 2 cut(s) 410, 535
Hpy8I GTNNAC 2 cut(s) 9, 76
Hpy99I CGWCG 8 cut(s) 38, 41, 62, 65, 68, 107, 110, 334
HpyAV CCTTC 2 cut(s) 181, 253
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 2 cut(s) 11, 39
HpyCH4V TGCA 2 cut(s) 397, 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 238, 304, 385, 394, 525
HpyF3I CTNAG 4 cut(s) 159, 210, 408, 489
HpySE526I ACGT 2 cut(s) 11, 39
Hsp92I GRCGYC 2 cut(s) 94, 329
Hsp92II CATG 1 cut(s) 538
HspAI GCGC 2 cut(s) 82, 94
KasI GGCGCC 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 498
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 4 cut(s) 142, 417, 498, 539
LmnI GCTCC 3 cut(s) 46, 54, 238
Lsp1109I GCAGC 2 cut(s) 319, 375
MaeII ACGT 2 cut(s) 11, 39
MaeIII GTNAC 2 cut(s) 443, 548
MalI GATC 4 cut(s) 144, 419, 500, 541
MboI GATC 4 cut(s) 142, 417, 498, 539
MboII GAAGA 6 cut(s) 100, 217, 286, 485, 491, 497
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 5 cut(s) 51, 234, 368, 521, 534
Mly113I GGCGCC 1 cut(s) 94
MlyI GAGTC 5 cut(s) 14, 170, 202, 289, 380
MnlI CCTC 9 cut(s) 84, 110, 135, 142, 174, 262, 346, 448, 469
MseI TTAA 1 cut(s) 336
MspA1I CMGCKG 2 cut(s) 20, 292
MspI CCGG 2 cut(s) 149, 169
MspR9I CCNGG 2 cut(s) 25, 149
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 3 cut(s) 20, 57, 528
MwoI GCNNNNNNNGC 5 cut(s) 238, 304, 385, 394, 525
NarI GGCGCC 1 cut(s) 94
NciI CCSGG 1 cut(s) 149
NdeII GATC 4 cut(s) 142, 417, 498, 539
NlaIII CATG 1 cut(s) 538
NlaIV GGNNCC 3 cut(s) 95, 320, 359
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 443, 548
PagI TCATGA 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 2 cut(s) 286, 413
Pfl23II CGTACG 1 cut(s) 467
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 8 cut(s) 22, 56, 98, 139, 294, 297, 309, 390
PleI GAGTC 5 cut(s) 13, 169, 202, 288, 379
PluTI GGCGCC 1 cut(s) 97
PpsI GAGTC 5 cut(s) 13, 169, 202, 288, 379
Psp124BI GAGCTC 2 cut(s) 51, 534
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PspLI CGTACG 1 cut(s) 467
PspN4I GGNNCC 3 cut(s) 95, 320, 359
PspPI GGNCC 1 cut(s) 319
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 2 cut(s) 215, 469
RsaNI GTAC 2 cut(s) 214, 468
SacI GAGCTC 2 cut(s) 51, 534
SacII CCGCGG 1 cut(s) 21
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
Sau3AI GATC 4 cut(s) 142, 417, 498, 539
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 5 cut(s) 14, 170, 202, 289, 380
ScrFI CCNGG 2 cut(s) 25, 149
SduI GDGCHC 5 cut(s) 51, 234, 368, 521, 534
SetI ASST 9 cut(s) 14, 42, 51, 204, 243, 390, 409, 490, 534
SfoI GGCGCC 1 cut(s) 95
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 1 cut(s) 319
SspDI GGCGCC 1 cut(s) 93
SstI GAGCTC 2 cut(s) 51, 534
StyD4I CCNGG 2 cut(s) 23, 147
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 2 cut(s) 14, 42
TaqI TCGA 3 cut(s) 102, 248, 416
TatI WGTACW 1 cut(s) 213
TauI GCSGC 6 cut(s) 23, 57, 99, 140, 295, 298
TfiI GAWTC 2 cut(s) 286, 413
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 2 cut(s) 239, 368
TseFI GTSAC 2 cut(s) 443, 548
TseI GCWGC 2 cut(s) 307, 388
Tsp45I GTSAC 2 cut(s) 443, 548
TspGWI ACGGA 2 cut(s) 117, 485
TspRI CASTG 2 cut(s) 239, 368
VpaK11BI GGWCC 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.