Rmu_sc0033776.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0033776.1
OG0014257
Physical Location & Seq
Forward (+)
9915 .. 10340
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0033776.1_g000004.1.cds

Sequence Viewer

Length: 426 bp
atggagtcaacatcatccgcggcctggcaaaacgacgacgtatgggagctccgccgcgacgacgacggcttcgtctacaagcgcaagaagaggcgccgcctcgacgacgaccccgccccaccgcctccgtcctccgccgcagatctcccggacgacaccgaggcccgccggagagagtggagggagaaggctctgctgaagctgaagactcagtacacggccgagattgagcactgggagcttttatcgaacaggctgaaggaaatggaggagaagacgagtcagattcagcggcggcgattagagcagcgagaacgggaccaaacggcgtcgtttgatggttctggcgtggaggggaaccagtgctcggagtcggtgctggacgagctgctcttgcaggtaattgaggttctgtttgttttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

16.62

Weight (kDa)

4.91

Isoelectric Point (pI)

67.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27520
fragaria_vesca FvH4_6g51790
malus_domestica MD09G1022900.v1.1 MD17G1022200.v1.1
prunus_persica Prupe.3G293900_v2.0.a1
pyrus_communis pycom111g01920
rosa_chinensis RchiOBHm_Chr2g0173291
rosa_laevigata RLG00000022177
rosa_multiflora Rmu_sc0010961.1_g000006 Rmu_sc0033776.1_g000004
rosa_roxburghii Rroxscaffold_2G00079070
rosa_rugosa Rorug02G0567100.1
rosa_samantha Rh2AG647500 Rh2BG658000 Rh2CG623400 Rh2DG671800
rosa_wichuraiana Rw2G053150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 388
AccB1I GGYRCC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 75
AccII CGCG 2 cut(s) 20, 57
AcoI YGGCCR 1 cut(s) 219
AcuI CTGAAG 3 cut(s) 218, 224, 278
AcyI GRCGYC 2 cut(s) 94, 329
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 2 cut(s) 24, 367
AjnI CCWGG 1 cut(s) 23
AluBI AGCT 4 cut(s) 49, 202, 241, 388
AluI AGCT 4 cut(s) 49, 202, 241, 388
Alw21I GWGCWC 3 cut(s) 51, 234, 368
AoxI GGCC 3 cut(s) 21, 162, 219
ApeKI GCWGC 2 cut(s) 307, 388
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 2 cut(s) 84, 96
AspS9I GGNCC 2 cut(s) 163, 319
AsuC2I CCSGG 1 cut(s) 149
AvaII GGWCC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 93
BanII GRGCYC 1 cut(s) 51
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BbsI GAAGAC 2 cut(s) 212, 281
Bbv12I GWGCWC 3 cut(s) 51, 234, 368
BbvI GCAGC 2 cut(s) 319, 375
BccI CCATC 1 cut(s) 332
BceAI ACGGC 3 cut(s) 82, 234, 342
BciT130I CCWGG 1 cut(s) 25
BcnI CCSGG 1 cut(s) 149
BfaI CTAG 1 cut(s) 424
BfoI RGCGCY 1 cut(s) 97
BfuAI ACCTGC 1 cut(s) 388
BglII AGATCT 1 cut(s) 142
BisI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
BlsI GCNGC 8 cut(s) 22, 56, 98, 139, 294, 297, 309, 390
Bme1390I CCNGG 2 cut(s) 25, 149
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 163, 319
BmiI GGNNCC 3 cut(s) 95, 320, 359
BmrFI CCNGG 2 cut(s) 25, 149
BmrI ACTGGG 1 cut(s) 244
BmuI ACTGGG 1 cut(s) 244
BpiI GAAGAC 2 cut(s) 212, 281
BpuMI CCSGG 1 cut(s) 149
BsaHI GRCGYC 2 cut(s) 94, 329
BsaJI CCNNGG 2 cut(s) 18, 159
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 367
Bse1I ACTGG 2 cut(s) 239, 361
BseBI CCWGG 1 cut(s) 25
BseDI CCNNGG 2 cut(s) 18, 159
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 2 cut(s) 24, 367
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 2 cut(s) 239, 361
BseRI GAGGAG 1 cut(s) 284
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 2 cut(s) 319, 375
Bsh1236I CGCG 2 cut(s) 20, 57
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 3 cut(s) 23, 164, 221
BshNI GGYRCC 1 cut(s) 93
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 3 cut(s) 51, 234, 368
BsiSI CCGG 2 cut(s) 149, 169
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 24, 367
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 3 cut(s) 23, 164, 221
Bsp1286I GDGCHC 3 cut(s) 51, 234, 368
Bsp143I GATC 1 cut(s) 142
BspANI GGCC 3 cut(s) 23, 164, 221
BspCNI CTCAG 1 cut(s) 223
BspFNI CGCG 2 cut(s) 20, 57
BspLI GGNNCC 3 cut(s) 95, 320, 359
BspMI ACCTGC 1 cut(s) 388
BspT107I GGYRCC 1 cut(s) 93
BsrI ACTGG 2 cut(s) 239, 361
BssECI CCNNGG 2 cut(s) 18, 159
BssMI GATC 1 cut(s) 142
BssNI GRCGYC 2 cut(s) 94, 329
Bst2UI CCWGG 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 2 cut(s) 94, 329
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 2 cut(s) 20, 57
BstH2I RGCGCY 1 cut(s) 97
BstHHI GCGC 2 cut(s) 84, 96
BstKTI GATC 1 cut(s) 145
BstMBI GATC 1 cut(s) 142
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 4 cut(s) 238, 304, 385, 394
BstNI CCWGG 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 23, 147
BstUI CGCG 2 cut(s) 20, 57
BstV1I GCAGC 2 cut(s) 319, 375
BstV2I GAAGAC 2 cut(s) 212, 281
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BstZI CGGCCG 1 cut(s) 219
BsuRI GGCC 3 cut(s) 23, 164, 221
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 14
BtsIMutI CAGTG 2 cut(s) 232, 368
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 166
CfoI GCGC 2 cut(s) 84, 96
Cfr13I GGNCC 2 cut(s) 163, 319
Cfr42I CCGCGG 1 cut(s) 21
CseI GACGC 1 cut(s) 318
Csp6I GTAC 1 cut(s) 214
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 210
DinI GGCGCC 1 cut(s) 95
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 219
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 2 cut(s) 41, 124
Ecl136II GAGCTC 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 319
Eco52I CGGCCG 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 3 cut(s) 218, 224, 278
EcoICRI GAGCTC 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 51
EgeI GGCGCC 1 cut(s) 95
EheI GGCGCC 1 cut(s) 95
FaiI YATR 1 cut(s) 43
FaqI GGGAC 1 cut(s) 332
FauI CCCGC 2 cut(s) 121, 173
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
FspBI CTAG 1 cut(s) 424
GlaI GCGC 2 cut(s) 83, 95
GluI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
HaeII RGCGCY 1 cut(s) 97
HaeIII GGCC 3 cut(s) 23, 164, 221
HapII CCGG 2 cut(s) 149, 169
HgaI GACGC 1 cut(s) 318
HhaI GCGC 2 cut(s) 84, 96
Hin1I GRCGYC 2 cut(s) 94, 329
Hin6I GCGC 2 cut(s) 82, 94
HinP1I GCGC 2 cut(s) 82, 94
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 5 cut(s) 5, 208, 280, 286, 371
HpaII CCGG 2 cut(s) 149, 169
Hpy166II GTNNAC 3 cut(s) 9, 76, 216
Hpy188I TCNGA 2 cut(s) 285, 370
Hpy8I GTNNAC 3 cut(s) 9, 76, 216
Hpy99I CGWCG 8 cut(s) 38, 41, 62, 65, 68, 107, 110, 334
HpyAV CCTTC 2 cut(s) 181, 253
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 304, 385, 394
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 2 cut(s) 94, 329
HspAI GCGC 2 cut(s) 82, 94
KasI GGCGCC 1 cut(s) 93
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 1 cut(s) 142
LmnI GCTCC 3 cut(s) 46, 54, 238
Lsp1109I GCAGC 2 cut(s) 319, 375
MaeI CTAG 1 cut(s) 424
MaeII ACGT 1 cut(s) 39
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 3 cut(s) 100, 217, 286
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 3 cut(s) 51, 234, 368
MluCI AATT 1 cut(s) 402
Mly113I GGCGCC 1 cut(s) 94
MlyI GAGTC 4 cut(s) 14, 202, 289, 380
MnlI CCTC 9 cut(s) 84, 110, 135, 142, 154, 174, 262, 346, 400
MspA1I CMGCKG 2 cut(s) 20, 292
MspI CCGG 2 cut(s) 149, 169
MspR9I CCNGG 2 cut(s) 25, 149
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 2 cut(s) 20, 57
MwoI GCNNNNNNNGC 4 cut(s) 238, 304, 385, 394
NarI GGCGCC 1 cut(s) 94
NciI CCSGG 1 cut(s) 149
NdeII GATC 1 cut(s) 142
NlaIV GGNNCC 3 cut(s) 95, 320, 359
NmeAIII GCCGAG 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 8 cut(s) 22, 56, 98, 139, 294, 297, 309, 390
PleI GAGTC 4 cut(s) 13, 202, 288, 379
PluTI GGCGCC 1 cut(s) 97
PpsI GAGTC 4 cut(s) 13, 202, 288, 379
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PspN4I GGNNCC 3 cut(s) 95, 320, 359
PspPI GGNCC 2 cut(s) 163, 319
PsrI GAACNNNNNNTAC 2 cut(s) 393, 425
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 1 cut(s) 51
SacII CCGCGG 1 cut(s) 21
SatI GCNGC 8 cut(s) 21, 55, 97, 138, 293, 296, 308, 389
Sau3AI GATC 1 cut(s) 142
Sau96I GGNCC 2 cut(s) 163, 319
SchI GAGTC 4 cut(s) 14, 202, 289, 380
ScrFI CCNGG 2 cut(s) 25, 149
SduI GDGCHC 3 cut(s) 51, 234, 368
SetI ASST 7 cut(s) 42, 51, 204, 243, 390, 402, 411
SfoI GGCGCC 1 cut(s) 95
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 1 cut(s) 319
Sse9I AATT 1 cut(s) 402
SspDI GGCGCC 1 cut(s) 93
SspMI CTAG 1 cut(s) 424
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 23, 147
TaiI ACGT 1 cut(s) 42
TaqI TCGA 2 cut(s) 102, 248
TasI AATT 1 cut(s) 402
TatI WGTACW 1 cut(s) 213
TauI GCSGC 6 cut(s) 23, 57, 99, 140, 295, 298
TfiI GAWTC 1 cut(s) 286
TscAI CASTG 2 cut(s) 239, 368
TseI GCWGC 2 cut(s) 307, 388
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 2 cut(s) 239, 368
VpaK11BI GGWCC 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.