MD09G1022900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014257
Physical Location & Seq
Forward (+)
1368005 .. 1369164
1160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1022900.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGAGTCTGCAGCAGCTTGGGTGGACGCCCAAGAGTGGGAGGTCTGCAACGACGACGGCTTCGTCTTCAAGCGGAGGAAGCGGCGCAGGGTCGACCCAGATGCCGACGAGTCTGTCCCGGCTCCACCGTCCTCGTCCGCCGCGGATCTACAGGAGGATCCCCAGAAACTCCGGAGAGAGCGGAGGAGGAAGTTTCTTCTCAAACTCAGAGATAAGCACCAGGCGGAGATCGACCAGTGGGAACTTTTGTCGAGCACGTTGCGCGCAATGGAGGAGAAGGCGCGGCAGCTTCAGCGGCAACGAGAGGAGGAGAAGCAGCATAGAATAGAAGAGCGAGAACGGGAGGATACGGCGTCGTTGGATAGGTCTGGGGTGTTAAAGATTCAGGGGACGGAGTCCGGTTGTGGGTCGTTGGTGGATGAGCTTCTCTTGCAGGTAGACGCACACGCAGCGATCATTGGTGATGTTTCGAGTCTCTGTGACACAGCAGAAGCACTATGTAAGGAAGAAGAGGAACAGATTACTCCGCTTTTCTTCGGTATTCCAATCTGCAGTTCACCGAGGGAGCTCATGAGATCGCTACAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

22.57

Weight (kDa)

4.99

Isoelectric Point (pI)

72.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27520
fragaria_vesca FvH4_6g51790
malus_domestica MD09G1022900.v1.1 MD17G1022200.v1.1
prunus_persica Prupe.3G293900_v2.0.a1
pyrus_communis pycom111g01920
rosa_chinensis RchiOBHm_Chr2g0173291
rosa_laevigata RLG00000022177
rosa_multiflora Rmu_sc0010961.1_g000006 Rmu_sc0033776.1_g000004
rosa_roxburghii Rroxscaffold_2G00079070
rosa_rugosa Rorug02G0567100.1
rosa_samantha Rh2AG647500 Rh2BG658000 Rh2CG623400 Rh2DG671800
rosa_wichuraiana Rw2G053150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 62, 113
Acc36I ACCTGC 1 cut(s) 424
AccBSI CCGCTC 1 cut(s) 181
AccI GTMKAC 2 cut(s) 93, 438
AccII CGCG 3 cut(s) 143, 264, 283
AccIII TCCGGA 1 cut(s) 171
AclWI GGATC 3 cut(s) 152, 153, 165
AcuI CTGAAG 1 cut(s) 275
AcyI GRCGYC 2 cut(s) 27, 353
AfiI CCNNNNNNNGG 3 cut(s) 36, 37, 405
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 538, 570
AluBI AGCT 4 cut(s) 17, 289, 424, 568
AluI AGCT 4 cut(s) 17, 289, 424, 568
Alw21I GWGCWC 2 cut(s) 257, 570
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 3 cut(s) 152, 153, 165
Aor13HI TCCGGA 1 cut(s) 171
ApeKI GCWGC 5 cut(s) 11, 14, 286, 316, 449
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 4 cut(s) 87, 264, 266, 283
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 473, 549
BamHI GGATCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 2 cut(s) 257, 570
BbvI GCAGC 5 cut(s) 23, 26, 298, 328, 461
BceAI ACGGC 2 cut(s) 73, 366
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 241, 275
BciT130I CCWGG 1 cut(s) 221
BciVI GTATCC 1 cut(s) 340
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 479
BfmI CTRYAG 3 cut(s) 9, 149, 550
BfuAI ACCTGC 1 cut(s) 424
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 9 cut(s) 12, 15, 83, 141, 284, 287, 296, 317, 450
BlsI GCNGC 9 cut(s) 13, 16, 84, 142, 285, 288, 297, 318, 451
Bme1390I CCNGG 2 cut(s) 119, 221
BmiI GGNNCC 2 cut(s) 123, 159
BmrFI CCNGG 2 cut(s) 119, 221
BmsI GCATC 1 cut(s) 91
BpiI GAAGAC 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 119
BsaHI GRCGYC 2 cut(s) 27, 353
BsaJI CCNNGG 2 cut(s) 141, 560
BsaWI WCCGGW 2 cut(s) 171, 398
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 37, 405
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 273
BseAI TCCGGA 1 cut(s) 171
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 141, 560
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 3 cut(s) 36, 37, 405
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 220
BseNI ACTGG 1 cut(s) 235
BsePI GCGCGC 1 cut(s) 262
BseRI GAGGAG 4 cut(s) 199, 287, 320, 323
BseXI GCAGC 5 cut(s) 23, 26, 298, 328, 461
Bsh1236I CGCG 3 cut(s) 143, 264, 283
BsiHKAI GWGCWC 2 cut(s) 257, 570
BsiSI CCGG 3 cut(s) 119, 172, 399
BslFI GGGAC 2 cut(s) 101, 403
BslI CCNNNNNNNGG 3 cut(s) 36, 37, 405
BsmAI GTCTC 1 cut(s) 479
BsmFI GGGAC 2 cut(s) 101, 403
Bsp1286I GDGCHC 2 cut(s) 257, 570
Bsp13I TCCGGA 1 cut(s) 171
Bsp143I GATC 5 cut(s) 145, 157, 228, 453, 575
BspCNI CTCAG 1 cut(s) 219
BspEI TCCGGA 1 cut(s) 171
BspFNI CGCG 3 cut(s) 143, 264, 283
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 2 cut(s) 123, 159
BspMAI CTGCAG 2 cut(s) 13, 554
BspMI ACCTGC 1 cut(s) 424
BspPI GGATC 3 cut(s) 152, 153, 165
BspQI GCTCTTC 1 cut(s) 324
BsrBI CCGCTC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 2 cut(s) 141, 560
BssHII GCGCGC 1 cut(s) 262
BssMI GATC 5 cut(s) 145, 157, 228, 453, 575
BssNI GRCGYC 2 cut(s) 27, 353
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 2 cut(s) 324, 504
BstACI GRCGYC 2 cut(s) 27, 353
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 1 cut(s) 206
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 3 cut(s) 143, 264, 283
BstHHI GCGC 4 cut(s) 87, 264, 266, 283
BstKTI GATC 5 cut(s) 148, 160, 231, 456, 578
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 5 cut(s) 145, 157, 228, 453, 575
BstMWI GCNNNNNNNGC 6 cut(s) 79, 261, 292, 295, 430, 449
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 117, 219
BstSFI CTRYAG 3 cut(s) 9, 149, 550
BstUI CGCG 3 cut(s) 143, 264, 283
BstV1I GCAGC 5 cut(s) 23, 26, 298, 328, 461
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 2 cut(s) 145, 157
BstYI RGATCY 2 cut(s) 145, 157
BsuI GTATCC 1 cut(s) 340
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 242
BveI ACCTGC 1 cut(s) 424
Cac8I GCNNGC 1 cut(s) 264
CciI TCATGA 1 cut(s) 570
CfoI GCGC 4 cut(s) 87, 264, 266, 283
Cfr42I CCGCGG 1 cut(s) 144
CseI GACGC 3 cut(s) 35, 342, 449
CviAII CATG 1 cut(s) 571
CviJI RGCY 6 cut(s) 17, 60, 122, 289, 424, 568
CviKI_1 RGCY 6 cut(s) 17, 60, 122, 289, 424, 568
DdeI CTNAG 1 cut(s) 206
DpnI GATC 5 cut(s) 147, 159, 230, 455, 577
DpnII GATC 5 cut(s) 145, 157, 228, 453, 575
DrdI GACNNNNNNGTC 2 cut(s) 62, 113
DseDI GACNNNNNNGTC 2 cut(s) 62, 113
Eam1104I CTCTTC 2 cut(s) 324, 504
EarI CTCTTC 2 cut(s) 324, 504
EciI GGCGGA 2 cut(s) 127, 239
Ecl136II GAGCTC 1 cut(s) 568
Eco24I GRGCYC 1 cut(s) 570
Eco53kI GAGCTC 1 cut(s) 568
Eco57I CTGAAG 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 570
FaeI CATG 1 cut(s) 574
FaiI YATR 3 cut(s) 321, 499, 572
FaqI GGGAC 2 cut(s) 101, 403
FatI CATG 1 cut(s) 570
FblI GTMKAC 2 cut(s) 93, 438
Fnu4HI GCNGC 9 cut(s) 12, 15, 83, 141, 284, 287, 296, 317, 450
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 570
Fsp4HI GCNGC 9 cut(s) 12, 15, 83, 141, 284, 287, 296, 317, 450
GlaI GCGC 4 cut(s) 86, 263, 265, 282
GluI GCNGC 9 cut(s) 12, 15, 83, 141, 284, 287, 296, 317, 450
HapII CCGG 3 cut(s) 119, 172, 399
HgaI GACGC 3 cut(s) 35, 342, 449
HhaI GCGC 4 cut(s) 87, 264, 266, 283
Hin1I GRCGYC 2 cut(s) 27, 353
Hin1II CATG 1 cut(s) 574
Hin6I GCGC 4 cut(s) 85, 262, 264, 281
HinP1I GCGC 4 cut(s) 85, 262, 264, 281
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 5 cut(s) 5, 110, 382, 395, 472
HpaII CCGG 3 cut(s) 119, 172, 399
HphI GGTGA 2 cut(s) 473, 549
Hpy166II GTNNAC 4 cut(s) 25, 94, 439, 557
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 2 cut(s) 172, 571
Hpy8I GTNNAC 4 cut(s) 25, 94, 439, 557
Hpy99I CGWCG 4 cut(s) 56, 59, 110, 358
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 4 cut(s) 11, 48, 433, 552
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 261, 292, 295, 430, 449
HpyF3I CTNAG 1 cut(s) 206
HpySE526I ACGT 1 cut(s) 257
Hsp92I GRCGYC 2 cut(s) 27, 353
Hsp92II CATG 1 cut(s) 574
HspAI GCGC 4 cut(s) 85, 262, 264, 281
Kpn2I TCCGGA 1 cut(s) 171
KspI CCGCGG 1 cut(s) 144
Kzo9I GATC 5 cut(s) 145, 157, 228, 453, 575
LguI GCTCTTC 1 cut(s) 324
LmnI GCTCC 2 cut(s) 127, 565
Lsp1109I GCAGC 5 cut(s) 23, 26, 298, 328, 461
LweI GCATC 1 cut(s) 91
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 1 cut(s) 479
MalI GATC 5 cut(s) 147, 159, 230, 455, 577
MbiI CCGCTC 1 cut(s) 181
MboI GATC 5 cut(s) 145, 157, 228, 453, 575
MboII GAAGA 6 cut(s) 58, 188, 341, 518, 521, 526
MflI RGATCY 2 cut(s) 145, 157
MhlI GDGCHC 2 cut(s) 257, 570
MlyI GAGTC 4 cut(s) 14, 119, 404, 481
MmeI TCCRAC 1 cut(s) 339
MroI TCCGGA 1 cut(s) 171
MseI TTAA 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 143, 295
MspI CCGG 3 cut(s) 119, 172, 399
MspR9I CCNGG 2 cut(s) 119, 221
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 3 cut(s) 143, 264, 283
MwoI GCNNNNNNNGC 6 cut(s) 79, 261, 292, 295, 430, 449
NciI CCSGG 1 cut(s) 119
NdeII GATC 5 cut(s) 145, 157, 228, 453, 575
NlaIII CATG 1 cut(s) 574
NlaIV GGNNCC 2 cut(s) 123, 159
NmuCI GTSAC 1 cut(s) 479
PagI TCATGA 1 cut(s) 570
PauI GCGCGC 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 1 cut(s) 382
PflFI GACNNNGTC 1 cut(s) 394
PkrI GCNGC 9 cut(s) 13, 16, 84, 142, 285, 288, 297, 318, 451
PleI GAGTC 4 cut(s) 13, 118, 403, 480
PpsI GAGTC 4 cut(s) 13, 118, 403, 480
Psp124BI GAGCTC 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 2 cut(s) 123, 159
PstI CTGCAG 2 cut(s) 13, 554
PsuI RGATCY 2 cut(s) 145, 157
PsyI GACNNNGTC 1 cut(s) 394
PteI GCGCGC 1 cut(s) 262
SacI GAGCTC 1 cut(s) 570
SacII CCGCGG 1 cut(s) 144
SalI GTCGAC 1 cut(s) 92
SapI GCTCTTC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 377
SatI GCNGC 9 cut(s) 12, 15, 83, 141, 284, 287, 296, 317, 450
Sau3AI GATC 5 cut(s) 145, 157, 228, 453, 575
SchI GAGTC 4 cut(s) 14, 119, 404, 481
ScrFI CCNGG 2 cut(s) 119, 221
SduI GDGCHC 2 cut(s) 257, 570
SetI ASST 8 cut(s) 19, 45, 260, 291, 368, 426, 438, 570
SfaNI GCATC 1 cut(s) 91
SfcI CTRYAG 3 cut(s) 9, 149, 550
Sfr303I CCGCGG 1 cut(s) 144
SgrBI CCGCGG 1 cut(s) 144
SstI GAGCTC 1 cut(s) 570
StyD4I CCNGG 2 cut(s) 117, 219
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 1 cut(s) 260
TaqI TCGA 4 cut(s) 93, 231, 251, 470
TauI GCSGC 4 cut(s) 85, 143, 286, 298
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 1 cut(s) 377
Tru9I TTAA 1 cut(s) 377
TscAI CASTG 1 cut(s) 242
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 5 cut(s) 11, 14, 286, 316, 449
Tsp45I GTSAC 1 cut(s) 479
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 1 cut(s) 242
Tth111I GACNNNGTC 1 cut(s) 394
XmiI GTMKAC 2 cut(s) 93, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.