AT3G28970

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013058
Physical Location & Seq
Reverse (-)
10987785 .. 10990016
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28970.2

Sequence Viewer

Length: 888 bp
ATGGATTCTTCGCCAGTTTCTGCTCGATTCGATATATTCGAGATTTATCGTAGATTCTGTGCTATTCGATCAGGACAACAATTGTGTAATAAGAAACCCTGTGATGGCGAAGAATCTCAAAGATTTAACTTGTCTAAAGAAGCAATAACTCAGCTCTTATATCTAGTAGAGAATAAGTTTCAAGCTAGGAACTCGATTTTTGATGAATTGTTTAAGTTGATGTCACGGCTAGACTTAATGGTGGACTTTACGGAGTTTACTTGCTTTTACGATTTTGTTTTCTTTATGTGCCGCGAGAATGGGCAAAAGAATATAACTATAAGTAGAGCTATTACTGCATGGAAACTTGTTTTGGCTGGGAGATTTAGGTTACTTAACCGCTGGTGTGACTTTATCGAGAAAAATCAACGGCACAATATTTCAGAAGATACTTGGCAACAAGTCTTGGCGTTTAGTCGTTGTGTACATGAGAATCTAGAAGGATATGACTCTGAAGGTGCATGGCCTGTCCTAATAGATGACTTTGTCGAGCATATGTACAGTATTTTAGGACCGAATAAGGACACTAGCTTGTTCTGCAAATGTGGTGACACAGAATCTGAATCATGCCTCTATCAAGAAGATGAGCATCACAAAGATTATCGCCGCCCACACACGGGTTTAAGAAACATTCCAGGTTTAAAGAGAAAAACATCGAAGAAGAATGACGAAGAAGAAGAAGATGAAGATGAAGAAGTGTTAGAAACCCAAAACTCTTCTTCTTTGCTAAACTTCAAACGAATCAAAACCAGCAACAGTCCAAGGTGTTCATCAAAGTCCCCTTGTTCAATCGAGCGAAGTCTTTCACAAGGTTTTGCGAGTCTTTTATCGACTGGAGATAAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

295

Amino Acids

34.37

Weight (kDa)

5.54

Isoelectric Point (pI)

60.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 82 - 166 3.4e-20 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 294
AciI CCGC 3 cut(s) 292, 379, 646
AcuI CTGAAG 1 cut(s) 513
AfaI GTAC 2 cut(s) 465, 539
AfiI CCNNNNNNNGG 3 cut(s) 104, 655, 656
AgsI TTSAA 3 cut(s) 182, 775, 828
AjnI CCWGG 1 cut(s) 673
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 4 cut(s) 154, 185, 329, 570
AluI AGCT 4 cut(s) 154, 185, 329, 570
AlwNI CAGNNNCTG 2 cut(s) 20, 599
AoxI GGCC 1 cut(s) 503
Asp700I GAANNNNTTC 1 cut(s) 841
AspS9I GGNCC 1 cut(s) 551
AsuHPI GGTGA 1 cut(s) 599
AvaII GGWCC 1 cut(s) 551
BccI CCATC 1 cut(s) 98
BceAI ACGGC 2 cut(s) 242, 425
BciT130I CCWGG 1 cut(s) 675
BfaI CTAG 5 cut(s) 164, 186, 230, 476, 567
BisI GCNGC 2 cut(s) 292, 646
BlsI GCNGC 2 cut(s) 293, 647
Bme1390I CCNGG 1 cut(s) 675
Bme18I GGWCC 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 551
BmrFI CCNGG 1 cut(s) 675
BmsI GCATC 1 cut(s) 637
BsaBI GATNNNNATC 1 cut(s) 627
BsaJI CCNNGG 1 cut(s) 800
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 655, 656
Bse1I ACTGG 2 cut(s) 14, 877
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 675
BseDI CCNNGG 1 cut(s) 800
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 3 cut(s) 104, 655, 656
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 2 cut(s) 14, 877
BseYI CCCAGC 1 cut(s) 356
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 1 cut(s) 505
BslFI GGGAC 1 cut(s) 802
BslI CCNNNNNNNGG 3 cut(s) 104, 655, 656
BsmFI GGGAC 1 cut(s) 802
BsnI GGCC 1 cut(s) 505
Bsp1407I TGTACA 2 cut(s) 463, 537
Bsp143I GATC 1 cut(s) 68
BspACI CCGC 3 cut(s) 292, 379, 646
BspANI GGCC 1 cut(s) 505
BspCNI CTCAG 1 cut(s) 163
BspFNI CGCG 1 cut(s) 294
BsrGI TGTACA 2 cut(s) 463, 537
BsrI ACTGG 2 cut(s) 14, 877
BssECI CCNNGG 1 cut(s) 800
BssMI GATC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 800
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 2 cut(s) 542, 797
Bst6I CTCTTC 1 cut(s) 760
BstAUI TGTACA 2 cut(s) 463, 537
BstDEI CTNAG 1 cut(s) 150
BstFNI CGCG 1 cut(s) 294
BstKTI GATC 1 cut(s) 71
BstMBI GATC 1 cut(s) 68
BstMWI GCNNNNNNNGC 2 cut(s) 335, 576
BstNI CCWGG 1 cut(s) 675
BstSCI CCNGG 1 cut(s) 673
BstUI CGCG 1 cut(s) 294
BsuRI GGCC 1 cut(s) 505
CaiI CAGNNNCTG 2 cut(s) 20, 599
Cfr13I GGNCC 1 cut(s) 551
Csp6I GTAC 2 cut(s) 464, 538
CviAII CATG 5 cut(s) 339, 467, 501, 606, 885
CviJI RGCY 7 cut(s) 154, 185, 229, 329, 356, 505, 570
CviKI_1 RGCY 7 cut(s) 154, 185, 229, 329, 356, 505, 570
CviQI GTAC 2 cut(s) 464, 538
DdeI CTNAG 1 cut(s) 150
DpnI GATC 1 cut(s) 70
DpnII GATC 1 cut(s) 68
DraI TTTAAA 1 cut(s) 681
Eam1104I CTCTTC 1 cut(s) 760
EarI CTCTTC 1 cut(s) 760
Eco130I CCWWGG 1 cut(s) 800
Eco47I GGWCC 1 cut(s) 551
Eco57I CTGAAG 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 800
ErhI CCWWGG 1 cut(s) 800
FaeI CATG 5 cut(s) 342, 470, 504, 609, 888
FaqI GGGAC 1 cut(s) 802
FatI CATG 5 cut(s) 338, 466, 500, 605, 884
FauNDI CATATG 1 cut(s) 534
Fnu4HI GCNGC 2 cut(s) 292, 646
Fsp4HI GCNGC 2 cut(s) 292, 646
FspBI CTAG 5 cut(s) 164, 186, 230, 476, 567
GluI GCNGC 2 cut(s) 292, 646
GsaI CCCAGC 1 cut(s) 360
HaeIII GGCC 1 cut(s) 505
Hin1II CATG 5 cut(s) 342, 470, 504, 609, 888
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 3 cut(s) 244, 258, 464
Hpy188I TCNGA 3 cut(s) 424, 493, 601
Hpy188III TCNNGA 5 cut(s) 40, 72, 397, 476, 617
Hpy8I GTNNAC 3 cut(s) 244, 258, 464
HpyAV CCTTC 2 cut(s) 473, 488
HpyCH4III ACNGT 2 cut(s) 542, 797
HpyCH4V TGCA 3 cut(s) 338, 500, 579
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 576
HpyF3I CTNAG 1 cut(s) 150
Hsp92II CATG 5 cut(s) 342, 470, 504, 609, 888
Kzo9I GATC 1 cut(s) 68
LweI GCATC 1 cut(s) 637
MaeI CTAG 5 cut(s) 164, 186, 230, 476, 567
MaeIII GTNAC 4 cut(s) 222, 369, 386, 587
MalI GATC 1 cut(s) 70
MboI GATC 1 cut(s) 68
MfeI CAATTG 1 cut(s) 80
MluCI AATT 2 cut(s) 80, 206
MlyI GAGTC 2 cut(s) 482, 868
MnlI CCTC 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 841
MseI TTAA 6 cut(s) 126, 213, 236, 375, 662, 680
MspA1I CMGCKG 1 cut(s) 381
MspR9I CCNGG 1 cut(s) 675
MunI CAATTG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 675
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 335, 576
NdeI CATATG 1 cut(s) 534
NdeII GATC 1 cut(s) 68
NlaIII CATG 5 cut(s) 342, 470, 504, 609, 888
NmuCI GTSAC 3 cut(s) 222, 386, 587
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 841
PfeI GAWTC 8 cut(s) 5, 27, 54, 113, 472, 596, 602, 780
PflFI GACNNNGTC 1 cut(s) 524
PkrI GCNGC 2 cut(s) 293, 647
PleI GAGTC 2 cut(s) 482, 867
PpsI GAGTC 2 cut(s) 482, 867
Psp6I CCWGG 1 cut(s) 673
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 1 cut(s) 673
PspPI GGNCC 1 cut(s) 551
PstNI CAGNNNCTG 2 cut(s) 20, 599
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 2 cut(s) 465, 539
RsaNI GTAC 2 cut(s) 464, 538
SaqAI TTAA 6 cut(s) 126, 213, 236, 375, 662, 680
SatI GCNGC 2 cut(s) 292, 646
Sau3AI GATC 1 cut(s) 68
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 2 cut(s) 482, 868
ScrFI CCNGG 1 cut(s) 675
SetI ASST 9 cut(s) 156, 187, 331, 371, 499, 572, 679, 806, 853
SfaNI GCATC 1 cut(s) 637
SinI GGWCC 1 cut(s) 551
Sse9I AATT 2 cut(s) 80, 206
SsiI CCGC 3 cut(s) 292, 379, 646
SspI AATATT 1 cut(s) 418
SspMI CTAG 5 cut(s) 164, 186, 230, 476, 567
StyD4I CCNGG 1 cut(s) 673
StyI CCWWGG 1 cut(s) 800
TaaI ACNGT 2 cut(s) 542, 797
TaqII GACCGA 1 cut(s) 568
TasI AATT 2 cut(s) 80, 206
TatI WGTACW 2 cut(s) 463, 537
TauI GCSGC 2 cut(s) 294, 648
TfiI GAWTC 8 cut(s) 5, 27, 54, 113, 472, 596, 602, 780
Tru1I TTAA 6 cut(s) 126, 213, 236, 375, 662, 680
Tru9I TTAA 6 cut(s) 126, 213, 236, 375, 662, 680
TseFI GTSAC 3 cut(s) 222, 386, 587
Tsp45I GTSAC 3 cut(s) 222, 386, 587
TspDTI ATGAA 4 cut(s) 219, 738, 744, 798
TspGWI ACGGA 1 cut(s) 266
Tth111I GACNNNGTC 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 551
XbaI TCTAGA 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 841
XspI CTAG 5 cut(s) 164, 186, 230, 476, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.