Rh2AG601900

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013058
Physical Location & Seq
Forward (+)
83017526 .. 83021192
3667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG601900.1

Sequence Viewer

Length: 825 bp
ATGGATCCGTCGGCCTCGGCTTCTTCCGACATATACGAGATTTATCGCCGATACTGTGATATCAGAAGAGGAAATGGGTATGGGGAAGGTTACAGACAAAACGATGAATTGCTGGTCGTCCAATATTCAAAGGAGGCATTAACTCAGCTCTTAAATCTGGTGGACTCAAAACTGCGTGCTAGGGTATCACTCTTTGATGAAATTGGCAAGCTAATGTCACAGCTTGACCTGGTGGTAGACTTTTCAGAATTCTCGCGCTTCTATGAGTTTGTGTTTTTCTTATGCCGCGAAAGTGGTCAGAAGAGTATCAGTAAGTTTTTTTTTTTTTTTTGTTCCAGGCAGTATAATAATTCTCAATCTCTTTTATTAATTTCCAACAATAGCCATATGAAAATTCATTTTCCTTTCTTTGAGTTATTTGCAGCCGTACCTAAGGCGATTTCTGCATGGAGAATAGTCTTAGCTGGGAGGTTTCGCTTGCTGAATCAATGGTGTGAATTTGTTGAGAAAAATCAGCGGCATAACATCTCTGAAGATACTTGGCGGCAAGTTTTAGCTTTTAGTCGGTGTGTACATGAAAATTTGGAAGGGTATGATCCTGAAGGAGCTTGGCCTGTCTTAATAGATGACTTCGTTGAGCATATGTACAGGTTTTCGGGATCTAATGTTAATTCTAACTTTTGTAACTGTGGCGACTCAGAATCCTGGTCATGCATATATGATGAACCTCTACCTGGTACTTCAATCACTATTCTTTCAACCAGCTCCGTTGTCCTTTACCTTGACAGTTGCCAGATTTATAATTTTATATGTGATGGAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

274

Amino Acids

31.84

Weight (kDa)

5.1

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 144 - 214 6.8e-21 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 801
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 256, 288
AciI CCGC 3 cut(s) 286, 517, 544
AclWI GGATC 3 cut(s) 12, 590, 667
AcsI RAATTY 4 cut(s) 248, 393, 497, 580
AcuI CTGAAG 2 cut(s) 552, 621
AfaI GTAC 4 cut(s) 429, 573, 647, 739
AfiI CCNNNNNNNGG 1 cut(s) 734
AgsI TTSAA 3 cut(s) 129, 744, 759
AjnI CCWGG 4 cut(s) 228, 335, 704, 733
AluBI AGCT 7 cut(s) 148, 211, 223, 464, 557, 608, 765
AluI AGCT 7 cut(s) 148, 211, 223, 464, 557, 608, 765
AlwI GGATC 3 cut(s) 12, 590, 667
AoxI GGCC 2 cut(s) 12, 611
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 4 cut(s) 248, 393, 497, 580
AseI ATTAAT 1 cut(s) 368
AspLEI GCGC 1 cut(s) 258
AxyI CCTNAGG 1 cut(s) 432
BamHI GGATCC 1 cut(s) 4
BbvI GCAGC 1 cut(s) 434
BccI CCATC 1 cut(s) 809
BceAI ACGGC 1 cut(s) 410
BciT130I CCWGG 4 cut(s) 230, 337, 706, 735
BfaI CTAG 1 cut(s) 180
BisI GCNGC 4 cut(s) 286, 423, 518, 545
BlsI GCNGC 4 cut(s) 287, 424, 519, 546
Bme1390I CCNGG 4 cut(s) 230, 337, 706, 735
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 4 cut(s) 230, 337, 706, 735
BsaJI CCNNGG 1 cut(s) 15
Bsc4I CCNNNNNNNGG 1 cut(s) 734
Bse21I CCTNAGG 1 cut(s) 432
BseBI CCWGG 4 cut(s) 230, 337, 706, 735
BseDI CCNNGG 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 734
BseMII CTCAG 2 cut(s) 158, 711
BseXI GCAGC 1 cut(s) 434
BseYI CCCAGC 1 cut(s) 464
Bsh1236I CGCG 2 cut(s) 256, 288
BshFI GGCC 2 cut(s) 14, 613
BslI CCNNNNNNNGG 1 cut(s) 734
BsnI GGCC 2 cut(s) 14, 613
Bsp1407I TGTACA 2 cut(s) 571, 645
Bsp143I GATC 3 cut(s) 4, 595, 659
BspACI CCGC 3 cut(s) 286, 517, 544
BspANI GGCC 2 cut(s) 14, 613
BspCNI CTCAG 2 cut(s) 157, 710
BspFNI CGCG 2 cut(s) 256, 288
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 3 cut(s) 12, 590, 667
BsrGI TGTACA 2 cut(s) 571, 645
BssECI CCNNGG 1 cut(s) 15
BssMI GATC 3 cut(s) 4, 595, 659
Bst2UI CCWGG 4 cut(s) 230, 337, 706, 735
Bst4CI ACNGT 3 cut(s) 56, 689, 788
Bst6I CTCTTC 2 cut(s) 61, 296
BstAUI TGTACA 2 cut(s) 571, 645
BstC8I GCNNGC 3 cut(s) 177, 209, 479
BstDEI CTNAG 4 cut(s) 144, 432, 460, 697
BstFNI CGCG 2 cut(s) 256, 288
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 3 cut(s) 7, 598, 662
BstMBI GATC 3 cut(s) 4, 595, 659
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 4 cut(s) 230, 337, 706, 735
BstSCI CCNGG 4 cut(s) 228, 335, 704, 733
BstUI CGCG 2 cut(s) 256, 288
BstV1I GCAGC 1 cut(s) 434
BstX2I RGATCY 2 cut(s) 4, 659
BstYI RGATCY 2 cut(s) 4, 659
Bsu36I CCTNAGG 1 cut(s) 432
BsuRI GGCC 2 cut(s) 14, 613
Cac8I GCNNGC 3 cut(s) 177, 209, 479
CfoI GCGC 1 cut(s) 258
CsiI ACCWGGT 2 cut(s) 228, 733
Csp6I GTAC 4 cut(s) 428, 572, 646, 738
CviAII CATG 3 cut(s) 447, 575, 711
CviQI GTAC 4 cut(s) 428, 572, 646, 738
DdeI CTNAG 4 cut(s) 144, 432, 460, 697
DpnI GATC 3 cut(s) 6, 597, 661
DpnII GATC 3 cut(s) 4, 595, 659
Eam1104I CTCTTC 2 cut(s) 61, 296
EarI CTCTTC 2 cut(s) 61, 296
Eco32I GATATC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 552, 621
Eco81I CCTNAGG 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 4 cut(s) 228, 335, 704, 733
EcoRV GATATC 1 cut(s) 61
EcoT22I ATGCAT 1 cut(s) 716
FaeI CATG 3 cut(s) 450, 578, 714
FatI CATG 3 cut(s) 446, 574, 710
FauNDI CATATG 2 cut(s) 387, 642
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 4 cut(s) 286, 423, 518, 545
Fsp4HI GCNGC 4 cut(s) 286, 423, 518, 545
FspBI CTAG 1 cut(s) 180
GlaI GCGC 1 cut(s) 257
GluI GCNGC 4 cut(s) 286, 423, 518, 545
GsaI CCCAGC 1 cut(s) 468
HaeIII GGCC 2 cut(s) 14, 613
HhaI GCGC 1 cut(s) 258
Hin1II CATG 3 cut(s) 450, 578, 714
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HinfI GANTC 4 cut(s) 164, 484, 695, 701
Hpy166II GTNNAC 3 cut(s) 163, 238, 572
Hpy188I TCNGA 6 cut(s) 28, 65, 247, 300, 532, 700
Hpy188III TCNNGA 2 cut(s) 599, 657
Hpy8I GTNNAC 3 cut(s) 163, 238, 572
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 80, 581, 596
HpyCH4III ACNGT 3 cut(s) 56, 689, 788
HpyCH4V TGCA 3 cut(s) 422, 446, 714
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 4 cut(s) 144, 432, 460, 697
Hsp92II CATG 3 cut(s) 450, 578, 714
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 3 cut(s) 4, 595, 659
LmnI GCTCC 2 cut(s) 605, 770
Lsp1109I GCAGC 1 cut(s) 434
MabI ACCWGGT 2 cut(s) 228, 733
MaeI CTAG 1 cut(s) 180
MaeIII GTNAC 3 cut(s) 89, 216, 683
MalI GATC 3 cut(s) 6, 597, 661
MboI GATC 3 cut(s) 4, 595, 659
MboII GAAGA 4 cut(s) 15, 78, 313, 545
MflI RGATCY 2 cut(s) 4, 659
MlyI GAGTC 2 cut(s) 158, 689
MmeI TCCRAC 2 cut(s) 51, 399
MnlI CCTC 5 cut(s) 25, 62, 127, 462, 738
Mph1103I ATGCAT 1 cut(s) 716
MseI TTAA 5 cut(s) 140, 152, 368, 620, 669
MspA1I CMGCKG 1 cut(s) 517
MspR9I CCNGG 4 cut(s) 230, 337, 706, 735
MvaI CCWGG 4 cut(s) 230, 337, 706, 735
MvnI CGCG 2 cut(s) 256, 288
MwoI GCNNNNNNNGC 1 cut(s) 443
NdeI CATATG 2 cut(s) 387, 642
NdeII GATC 3 cut(s) 4, 595, 659
NlaIII CATG 3 cut(s) 450, 578, 714
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 216
NsiI ATGCAT 1 cut(s) 716
PfeI GAWTC 2 cut(s) 484, 701
PkrI GCNGC 4 cut(s) 287, 424, 519, 546
PleI GAGTC 2 cut(s) 158, 689
PpsI GAGTC 2 cut(s) 158, 689
PshBI ATTAAT 1 cut(s) 368
PsiI TTATAA 1 cut(s) 801
Psp6I CCWGG 4 cut(s) 228, 335, 704, 733
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 4 cut(s) 228, 335, 704, 733
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 2 cut(s) 4, 659
RsaI GTAC 4 cut(s) 429, 573, 647, 739
RsaNI GTAC 4 cut(s) 428, 572, 646, 738
SaqAI TTAA 5 cut(s) 140, 152, 368, 620, 669
SatI GCNGC 4 cut(s) 286, 423, 518, 545
Sau3AI GATC 3 cut(s) 4, 595, 659
SchI GAGTC 2 cut(s) 158, 689
ScrFI CCNGG 4 cut(s) 230, 337, 706, 735
SexAI ACCWGGT 2 cut(s) 228, 733
SsiI CCGC 3 cut(s) 286, 517, 544
SspI AATATT 1 cut(s) 125
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 4 cut(s) 228, 335, 704, 733
TaaI ACNGT 3 cut(s) 56, 689, 788
TatI WGTACW 2 cut(s) 571, 645
TauI GCSGC 3 cut(s) 288, 520, 547
TfiI GAWTC 2 cut(s) 484, 701
Tru1I TTAA 5 cut(s) 140, 152, 368, 620, 669
Tru9I TTAA 5 cut(s) 140, 152, 368, 620, 669
TseFI GTSAC 1 cut(s) 216
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 6 cut(s) 120, 213, 386, 404, 591, 738
TspGWI ACGGA 1 cut(s) 757
VspI ATTAAT 1 cut(s) 368
XapI RAATTY 4 cut(s) 248, 393, 497, 580
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 1 cut(s) 180
Zsp2I ATGCAT 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.