Prupe.3G266600_v2.0.a1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0013058
Physical Location & Seq
Forward (+)
24965971 .. 24969814
3844 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G266600.2

Sequence Viewer

Length: 975 bp
ATGGATCCAACCGGGTCCACTCGTTTCGACATATTTGAGATTTATCGCCGATACTGTGATGTCAGGACAGTAAATGGATATGGTCATGGGGATGATTACAGAGGAAACAATGAATCACTGATGACTAAATATTCAAGGGAGGCATTAATTCAGCTCTTAAATCTGGTGCAGTTGAAACTACATCCTAGGATATCAATTTTTGATGAAGTTTTCAAGCTAATGTCACGGTTAGACTTGGTGGTAGACTTTTCTGAATTCTCGCGCTTCTATGATTTTGTTTTCTTTATGTGCCGTGAAAATGGTCAGAAGAATATCACTGTAAATAAGGCGATTACTGCATGGAGGTTAGTATTAGCTGGGAGGTTTCGGTTGCTGAATCAATGGTGTGATTTTGTTGAGAAAAATCAGCGGCATAACATCTCTGAAGATACCTGGAGGCAAGTTTTAGCTTTTAGCCGGTGTGTGCATGAAAATTTGGAAGGGTATGATCCTGAAGGAGCTTGGCCTGTTTTAATAGATGACTTTGTTGAACATATGTACAGGGTTTCAGGATCTAATGAAAATTCTAACTTCAACTGTAACTGTGGTGATTCAGAATCCTGGTCATGCACATATGATGATCCTCTTCCTGGCTTGAAGATTTTACCTGGTTTGAAGAGGAAGTTACCTGGAGACCTTCAAATTGATGAAATGGAGTCCTCAGCCACCCTCTTCCCCACACATCCTGCAGACTTGAAGCCTGCATTAAGCATTAAGAGAAGTCGGCCGATGTCTTACATACCAGCAAACTGGGAGGACAATACCACAGGAAGTACTGTAGATGGCTGCATGGAAACCATTAGACATAGCAGTCCATTGAATTCTTCCAAATCTCCATGTGCGGTTGAAGGTTGCCTGTTGAAGGGATTTGCAGGGCTGCTTTCAACACGTTCCTGTCTGCAGTTTGATCGGGACAGGGGAGTTTCATTTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

325

Amino Acids

37.11

Weight (kDa)

5.71

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 849
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 262
AciI CCGC 2 cut(s) 409, 881
AclWI GGATC 4 cut(s) 12, 482, 559, 614
AcoI YGGCCR 1 cut(s) 764
AcsI RAATTY 4 cut(s) 254, 472, 562, 859
AcuI CTGAAG 2 cut(s) 444, 513
AfaI GTAC 2 cut(s) 539, 814
AfiI CCNNNNNNNGG 2 cut(s) 629, 901
AflIII ACRYGT 1 cut(s) 926
AjnI CCWGG 5 cut(s) 431, 599, 628, 646, 667
AluBI AGCT 5 cut(s) 154, 217, 356, 449, 500
AluI AGCT 5 cut(s) 154, 217, 356, 449, 500
Alw26I GTCTC 1 cut(s) 666
AlwI GGATC 4 cut(s) 12, 482, 559, 614
AoxI GGCC 2 cut(s) 503, 764
ApeKI GCWGC 2 cut(s) 825, 916
ApoI RAATTY 4 cut(s) 254, 472, 562, 859
AseI ATTAAT 1 cut(s) 146
AspA2I CCTAGG 1 cut(s) 185
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 15
AsuC2I CCSGG 1 cut(s) 13
AsuHPI GGTGA 1 cut(s) 599
AvaII GGWCC 1 cut(s) 15
AvrII CCTAGG 1 cut(s) 185
BamHI GGATCC 1 cut(s) 4
BbvCI CCTCAGC 1 cut(s) 700
BbvI GCAGC 2 cut(s) 812, 903
BccI CCATC 1 cut(s) 815
BceAI ACGGC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 929, 963
BciT130I CCWGG 5 cut(s) 433, 601, 630, 648, 669
BcnI CCSGG 1 cut(s) 13
BcoDI GTCTC 1 cut(s) 666
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 3 cut(s) 726, 816, 938
BisI GCNGC 3 cut(s) 410, 826, 917
BlnI CCTAGG 1 cut(s) 185
BlsI GCNGC 3 cut(s) 411, 827, 918
BmcAI AGTACT 1 cut(s) 814
Bme1390I CCNGG 6 cut(s) 13, 433, 601, 630, 648, 669
Bme18I GGWCC 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 6, 16
BmrFI CCNGG 6 cut(s) 13, 433, 601, 630, 648, 669
BmrI ACTGGG 1 cut(s) 799
BmuI ACTGGG 1 cut(s) 799
BpmI CTGGAG 2 cut(s) 454, 690
Bpu10I CCTNAGC 1 cut(s) 700
BpuMI CCSGG 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 666
BsaJI CCNNGG 1 cut(s) 185
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 901
Bse118I RCCGGY 1 cut(s) 456
Bse1I ACTGG 1 cut(s) 794
BseBI CCWGG 5 cut(s) 433, 601, 630, 648, 669
BseDI CCNNGG 1 cut(s) 185
BseGI GGATG 3 cut(s) 97, 181, 721
BseLI CCNNNNNNNGG 2 cut(s) 629, 901
BseMII CTCAG 1 cut(s) 714
BseNI ACTGG 1 cut(s) 794
BseX3I CGGCCG 1 cut(s) 764
BseXI GCAGC 2 cut(s) 812, 903
BseYI CCCAGC 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 188
Bsh1236I CGCG 1 cut(s) 262
Bsh1285I CGRYCG 1 cut(s) 767
BshFI GGCC 2 cut(s) 505, 766
BsiEI CGRYCG 1 cut(s) 767
BsiSI CCGG 2 cut(s) 12, 457
BslFI GGGAC 1 cut(s) 965
BslI CCNNNNNNNGG 2 cut(s) 629, 901
BsmAI GTCTC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 965
BsnI GGCC 2 cut(s) 505, 766
Bso31I GGTCTC 1 cut(s) 666
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 5 cut(s) 4, 487, 551, 619, 946
BspACI CCGC 2 cut(s) 409, 881
BspANI GGCC 2 cut(s) 505, 766
BspCNI CTCAG 1 cut(s) 713
BspFNI CGCG 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 6, 16
BspMAI CTGCAG 2 cut(s) 730, 942
BspPI GGATC 4 cut(s) 12, 482, 559, 614
BspTNI GGTCTC 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 456
BsrGI TGTACA 1 cut(s) 537
BsrI ACTGG 1 cut(s) 794
BssAI RCCGGY 1 cut(s) 456
BssECI CCNNGG 1 cut(s) 185
BssMI GATC 5 cut(s) 4, 487, 551, 619, 946
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 5 cut(s) 433, 601, 630, 648, 669
Bst4CI ACNGT 7 cut(s) 56, 70, 228, 319, 578, 584, 817
Bst6I CTCTTC 3 cut(s) 630, 650, 716
BstAUI TGTACA 1 cut(s) 537
BstC8I GCNNGC 1 cut(s) 741
BstDEI CTNAG 1 cut(s) 700
BstENI CCTNNNNNAGG 1 cut(s) 899
BstF5I GGATG 3 cut(s) 97, 181, 721
BstFNI CGCG 1 cut(s) 262
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 5 cut(s) 7, 490, 554, 622, 949
BstMAI GTCTC 1 cut(s) 666
BstMBI GATC 5 cut(s) 4, 487, 551, 619, 946
BstMCI CGRYCG 1 cut(s) 767
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 5 cut(s) 433, 601, 630, 648, 669
BstSCI CCNGG 6 cut(s) 11, 431, 599, 628, 646, 667
BstSFI CTRYAG 3 cut(s) 726, 816, 938
BstUI CGCG 1 cut(s) 262
BstV1I GCAGC 2 cut(s) 812, 903
BstX2I RGATCY 2 cut(s) 4, 551
BstXI CCANNNNNNTGG 1 cut(s) 789
BstYI RGATCY 2 cut(s) 4, 551
BstZI CGGCCG 1 cut(s) 764
BsuRI GGCC 2 cut(s) 505, 766
BtsCI GGATG 3 cut(s) 97, 181, 721
BtsIMutI CAGTG 2 cut(s) 116, 315
Cac8I GCNNGC 1 cut(s) 741
CfoI GCGC 1 cut(s) 264
Cfr10I RCCGGY 1 cut(s) 456
Cfr13I GGNCC 1 cut(s) 15
CsiI ACCWGGT 1 cut(s) 646
Csp6I GTAC 2 cut(s) 538, 813
CviAII CATG 6 cut(s) 86, 339, 467, 606, 829, 876
CviQI GTAC 2 cut(s) 538, 813
DdeI CTNAG 1 cut(s) 700
DpnI GATC 5 cut(s) 6, 489, 553, 621, 948
DpnII GATC 5 cut(s) 4, 487, 551, 619, 946
DrdI GACNNNNNNGTC 1 cut(s) 849
DseDI GACNNNNNNGTC 1 cut(s) 849
EaeI YGGCCR 1 cut(s) 764
EagI CGGCCG 1 cut(s) 764
Eam1104I CTCTTC 3 cut(s) 630, 650, 716
EarI CTCTTC 3 cut(s) 630, 650, 716
EclXI CGGCCG 1 cut(s) 764
Eco130I CCWWGG 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 666
Eco32I GATATC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 15
Eco52I CGGCCG 1 cut(s) 764
Eco57I CTGAAG 2 cut(s) 444, 513
EcoNI CCTNNNNNAGG 1 cut(s) 899
EcoRI GAATTC 2 cut(s) 254, 859
EcoRII CCWGG 5 cut(s) 431, 599, 628, 646, 667
EcoRV GATATC 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 6 cut(s) 89, 342, 470, 609, 832, 879
FaqI GGGAC 1 cut(s) 965
FatI CATG 6 cut(s) 85, 338, 466, 605, 828, 875
FauNDI CATATG 2 cut(s) 534, 613
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 410, 826, 917
FokI GGATG 3 cut(s) 104, 168, 708
Fsp4HI GCNGC 3 cut(s) 410, 826, 917
FspBI CTAG 1 cut(s) 186
GlaI GCGC 1 cut(s) 263
GluI GCNGC 3 cut(s) 410, 826, 917
GsaI CCCAGC 1 cut(s) 360
GsuI CTGGAG 2 cut(s) 454, 690
HaeIII GGCC 2 cut(s) 505, 766
HapII CCGG 2 cut(s) 12, 457
HhaI GCGC 1 cut(s) 264
Hin1II CATG 6 cut(s) 89, 342, 470, 609, 832, 879
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HinfI GANTC 5 cut(s) 113, 376, 590, 596, 695
HpaII CCGG 2 cut(s) 12, 457
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 2 cut(s) 18, 244
Hpy188I TCNGA 4 cut(s) 253, 306, 424, 595
Hpy188III TCNNGA 4 cut(s) 64, 491, 549, 950
Hpy8I GTNNAC 2 cut(s) 18, 244
HpyAV CCTTC 5 cut(s) 473, 488, 686, 881, 895
HpyCH4III ACNGT 7 cut(s) 56, 70, 228, 319, 578, 584, 817
HpyCH4IV ACGT 1 cut(s) 928
HpyCH4V TGCA 9 cut(s) 169, 338, 466, 609, 728, 743, 828, 911, 940
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 1 cut(s) 700
HpySE526I ACGT 1 cut(s) 928
Hsp92II CATG 6 cut(s) 89, 342, 470, 609, 832, 879
HspAI GCGC 1 cut(s) 262
Kzo9I GATC 5 cut(s) 4, 487, 551, 619, 946
LmnI GCTCC 1 cut(s) 497
Lsp1109I GCAGC 2 cut(s) 812, 903
MabI ACCWGGT 1 cut(s) 646
MaeI CTAG 1 cut(s) 186
MaeII ACGT 1 cut(s) 928
MaeIII GTNAC 3 cut(s) 222, 578, 663
MalI GATC 5 cut(s) 6, 489, 553, 621, 948
MboI GATC 5 cut(s) 4, 487, 551, 619, 946
MboII GAAGA 7 cut(s) 319, 437, 617, 649, 667, 703, 855
MflI RGATCY 2 cut(s) 4, 551
MluCI AATT 7 cut(s) 147, 195, 254, 472, 562, 681, 859
MlyI GAGTC 1 cut(s) 704
MmeI TCCRAC 1 cut(s) 32
MseI TTAA 5 cut(s) 146, 158, 512, 746, 753
MslI CAYNNNNRTG 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 2 cut(s) 12, 457
MspR9I CCNGG 6 cut(s) 13, 433, 601, 630, 648, 669
MvaI CCWGG 5 cut(s) 433, 601, 630, 648, 669
MvnI CGCG 1 cut(s) 262
MwoI GCNNNNNNNGC 1 cut(s) 335
NciI CCSGG 1 cut(s) 13
NdeI CATATG 2 cut(s) 534, 613
NdeII GATC 5 cut(s) 4, 487, 551, 619, 946
NlaIII CATG 6 cut(s) 89, 342, 470, 609, 832, 879
NlaIV GGNNCC 2 cut(s) 6, 16
NmuCI GTSAC 1 cut(s) 222
PfeI GAWTC 4 cut(s) 113, 376, 590, 596
PkrI GCNGC 3 cut(s) 411, 827, 918
PleI GAGTC 1 cut(s) 703
PpsI GAGTC 1 cut(s) 703
PshBI ATTAAT 1 cut(s) 146
Psp6I CCWGG 5 cut(s) 431, 599, 628, 646, 667
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 5 cut(s) 431, 599, 628, 646, 667
PspN4I GGNNCC 2 cut(s) 6, 16
PspPI GGNCC 1 cut(s) 15
PstI CTGCAG 2 cut(s) 730, 942
PsuI RGATCY 2 cut(s) 4, 551
RsaI GTAC 2 cut(s) 539, 814
RsaNI GTAC 2 cut(s) 538, 813
RseI CAYNNNNRTG 1 cut(s) 90
SaqAI TTAA 5 cut(s) 146, 158, 512, 746, 753
SatI GCNGC 3 cut(s) 410, 826, 917
Sau3AI GATC 5 cut(s) 4, 487, 551, 619, 946
Sau96I GGNCC 1 cut(s) 15
ScaI AGTACT 1 cut(s) 814
SchI GAGTC 1 cut(s) 704
ScrFI CCNGG 6 cut(s) 13, 433, 601, 630, 648, 669
SexAI ACCWGGT 1 cut(s) 646
SfcI CTRYAG 3 cut(s) 726, 816, 938
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 7 cut(s) 147, 195, 254, 472, 562, 681, 859
SsiI CCGC 2 cut(s) 409, 881
SspI AATATT 1 cut(s) 131
SspMI CTAG 1 cut(s) 186
StyD4I CCNGG 6 cut(s) 11, 431, 599, 628, 646, 667
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 7 cut(s) 56, 70, 228, 319, 578, 584, 817
TaiI ACGT 1 cut(s) 931
TaqI TCGA 1 cut(s) 27
TasI AATT 7 cut(s) 147, 195, 254, 472, 562, 681, 859
TatI WGTACW 2 cut(s) 537, 812
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 4 cut(s) 113, 376, 590, 596
Tru1I TTAA 5 cut(s) 146, 158, 512, 746, 753
Tru9I TTAA 5 cut(s) 146, 158, 512, 746, 753
TscAI CASTG 2 cut(s) 123, 322
TseFI GTSAC 1 cut(s) 222
TseI GCWGC 2 cut(s) 825, 916
Tsp45I GTSAC 1 cut(s) 222
TspDTI ATGAA 6 cut(s) 126, 219, 483, 573, 702, 954
TspRI CASTG 2 cut(s) 123, 322
VpaK11BI GGWCC 1 cut(s) 15
VspI ATTAAT 1 cut(s) 146
XagI CCTNNNNNAGG 1 cut(s) 899
XapI RAATTY 4 cut(s) 254, 472, 562, 859
XmaJI CCTAGG 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 1 cut(s) 186
ZrmI AGTACT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.