Rroxscaffold_2G00083160

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013058
Physical Location & Seq
Reverse (-)
5877090 .. 5881081
3992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083160.1

Sequence Viewer

Length: 1047 bp
ATGGATCCGTCGGCCTCCGCTTCTTTCGACATATACGAGATTTATCGCCGATACTGTGATATCAGAAGAGGAAATGGGTATGGGGAAGGTTACAGACAAAACGATGAATCGCTGGTCGTCCAATATTCAAAGGAGGCATTAACTCAGCTCTTAAATCTGGTGGACTCAAAACTGCGTGCTAGGGTATCACTCTTTGATGAAATTGGCAAGCTAATGTCACAGCTTGACTTGGTGGTAGACTTTTCAGAATTCTCATGCTTCTATGAGTTTGTTTTTTTCTTATGCCGCGAAAGTGGTCAGAAGAGTATCACTGTACCTAAGGCGATTTCTGCATGGAGAATAGTATTAGCTGGGAGGTTTCGCTTGCTGAATCAATGGTGTGAATTTGTTGAGGTAACTAATTTTGGTATTCCTCACAGAAAAAATCAGCGGCATAACATCTCTGAAGATACTTGGCGGCAAGTTTTAGCTTTTAGCCGGTGTGTACATGAAAATTTGGAAGGGTATGATCCTGAAGGAGCTTGGCCTGTCTTAATAGATGACTTCGTTGAGCATATGTACAGGTTTTCGGGATCTAATGTTAATTCTAACTTCTGTAACTGTGGCGACTCAGAATCCTGGGCATGCATATATGATGAACCTCTACCTGGCTTGAAGAGTTATCCTGGTTTGAAGAGGAAAATACCAGGGGATGTTGATATTGATGAAATGGAGTCCTCACCTACCTCCTTCCCAGAGTGTACAGACTTGAATCCTACATTAAGCTTTAAGAGGAGTCGGGTGATGTCATACGGGCCAGCAAATGATGTACCAGGAAATCATGTTGACGAATGCATGGAAGTTATTAGACACAGCAGTCCATTAAATTCTTCCAAATCTCCATGTGCAGTTGAAGGTTGCCTGTCAAAGGGCTTTGCAGGGCTCTTTACGGGAGAGGGGTGTTTCATTTACATAGAAGTCACCAATATTCTTTCATCTGATCTATCTATTGAAGAATGCCTAGGCATTAGGGGATACAAGAAGGCGAAACCTCTGCATGGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

348

Amino Acids

39.38

Weight (kDa)

5.16

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 80 - 185 8e-28 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 855
AccI GTMKAC 1 cut(s) 237
AccII CGCG 1 cut(s) 288
AciI CCGC 4 cut(s) 18, 286, 430, 457
AclWI GGATC 3 cut(s) 12, 503, 580
AcsI RAATTY 4 cut(s) 248, 383, 493, 865
AcuI CTGAAG 2 cut(s) 465, 534
AfaI GTAC 5 cut(s) 315, 486, 560, 742, 810
AfiI CCNNNNNNNGG 3 cut(s) 647, 907, 1037
AgsI TTSAA 6 cut(s) 129, 655, 673, 751, 893, 992
AjnI CCWGG 5 cut(s) 617, 646, 664, 685, 811
AluBI AGCT 7 cut(s) 148, 211, 223, 350, 470, 521, 765
AluI AGCT 7 cut(s) 148, 211, 223, 350, 470, 521, 765
AlwI GGATC 3 cut(s) 12, 503, 580
AoxI GGCC 3 cut(s) 12, 524, 794
ApoI RAATTY 4 cut(s) 248, 383, 493, 865
AspA2I CCTAGG 1 cut(s) 1000
AspS9I GGNCC 1 cut(s) 794
AsuHPI GGTGA 3 cut(s) 711, 793, 952
AvrII CCTAGG 1 cut(s) 1000
AxyI CCTNAGG 1 cut(s) 318
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 924
BcgI CGANNNNNNTGC 1 cut(s) 1015
BciT130I CCWGG 5 cut(s) 619, 648, 666, 687, 813
BciVI GTATCC 1 cut(s) 1007
BfaI CTAG 2 cut(s) 180, 1001
BfuI GTATCC 1 cut(s) 1007
BisI GCNGC 3 cut(s) 286, 431, 458
BlnI CCTAGG 1 cut(s) 1000
BlsI GCNGC 3 cut(s) 287, 432, 459
Bme1390I CCNGG 5 cut(s) 619, 648, 666, 687, 813
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 5 cut(s) 619, 648, 666, 687, 813
BsaJI CCNNGG 3 cut(s) 618, 686, 1000
Bsc4I CCNNNNNNNGG 3 cut(s) 647, 907, 1037
Bse118I RCCGGY 1 cut(s) 477
Bse21I CCTNAGG 1 cut(s) 318
BseBI CCWGG 5 cut(s) 619, 648, 666, 687, 813
BseDI CCNNGG 3 cut(s) 618, 686, 1000
BseGI GGATG 1 cut(s) 697
BseLI CCNNNNNNNGG 3 cut(s) 647, 907, 1037
BseMII CTCAG 2 cut(s) 158, 624
BseRI GAGGAG 1 cut(s) 787
BseYI CCCAGC 1 cut(s) 350
BsgI GTGCAG 1 cut(s) 906
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 3 cut(s) 14, 526, 796
BsiSI CCGG 1 cut(s) 478
BslI CCNNNNNNNGG 3 cut(s) 647, 907, 1037
BsmI GAATGC 2 cut(s) 836, 1001
BsnI GGCC 3 cut(s) 14, 526, 796
Bsp1286I GDGCHC 1 cut(s) 924
Bsp1407I TGTACA 3 cut(s) 484, 558, 740
Bsp143I GATC 4 cut(s) 4, 508, 572, 979
BspACI CCGC 4 cut(s) 18, 286, 430, 457
BspANI GGCC 3 cut(s) 14, 526, 796
BspCNI CTCAG 2 cut(s) 157, 623
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 3 cut(s) 12, 503, 580
BsrFI RCCGGY 1 cut(s) 477
BsrGI TGTACA 3 cut(s) 484, 558, 740
BssAI RCCGGY 1 cut(s) 477
BssECI CCNNGG 3 cut(s) 618, 686, 1000
BssMI GATC 4 cut(s) 4, 508, 572, 979
BssT1I CCWWGG 1 cut(s) 1000
Bst2UI CCWGG 5 cut(s) 619, 648, 666, 687, 813
Bst4CI ACNGT 3 cut(s) 56, 313, 602
Bst6I CTCTTC 4 cut(s) 61, 296, 650, 668
BstAUI TGTACA 3 cut(s) 484, 558, 740
BstC8I GCNNGC 5 cut(s) 177, 209, 365, 625, 798
BstDEI CTNAG 3 cut(s) 144, 318, 610
BstENI CCTNNNNNAGG 1 cut(s) 905
BstF5I GGATG 1 cut(s) 697
BstFNI CGCG 1 cut(s) 288
BstKTI GATC 4 cut(s) 7, 511, 575, 982
BstMBI GATC 4 cut(s) 4, 508, 572, 979
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 5 cut(s) 619, 648, 666, 687, 813
BstNSI RCATGY 1 cut(s) 627
BstSCI CCNGG 5 cut(s) 617, 646, 664, 685, 811
BstUI CGCG 1 cut(s) 288
BstX2I RGATCY 2 cut(s) 4, 572
BstYI RGATCY 2 cut(s) 4, 572
Bsu36I CCTNAGG 1 cut(s) 318
BsuI GTATCC 1 cut(s) 1007
BsuRI GGCC 3 cut(s) 14, 526, 796
BtsCI GGATG 1 cut(s) 697
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 5 cut(s) 177, 209, 365, 625, 798
Cfr10I RCCGGY 1 cut(s) 477
Cfr13I GGNCC 1 cut(s) 794
Csp6I GTAC 5 cut(s) 314, 485, 559, 741, 809
CviAII CATG 8 cut(s) 255, 333, 488, 624, 821, 835, 882, 1037
CviQI GTAC 5 cut(s) 314, 485, 559, 741, 809
DdeI CTNAG 3 cut(s) 144, 318, 610
DpnI GATC 4 cut(s) 6, 510, 574, 981
DpnII GATC 4 cut(s) 4, 508, 572, 979
DrdI GACNNNNNNGTC 1 cut(s) 855
DseDI GACNNNNNNGTC 1 cut(s) 855
Eam1104I CTCTTC 4 cut(s) 61, 296, 650, 668
EarI CTCTTC 4 cut(s) 61, 296, 650, 668
Eco130I CCWWGG 1 cut(s) 1000
Eco24I GRGCYC 1 cut(s) 924
Eco32I GATATC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 465, 534
Eco81I CCTNAGG 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 905
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 5 cut(s) 617, 646, 664, 685, 811
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 1000
EcoT22I ATGCAT 2 cut(s) 629, 836
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 1 cut(s) 1000
FaeI CATG 8 cut(s) 258, 336, 491, 627, 824, 838, 885, 1040
FatI CATG 8 cut(s) 254, 332, 487, 623, 820, 834, 881, 1036
FauNDI CATATG 1 cut(s) 555
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 3 cut(s) 286, 431, 458
FokI GGATG 1 cut(s) 704
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 3 cut(s) 286, 431, 458
FspBI CTAG 2 cut(s) 180, 1001
GluI GCNGC 3 cut(s) 286, 431, 458
GsaI CCCAGC 1 cut(s) 354
HaeIII GGCC 3 cut(s) 14, 526, 796
HapII CCGG 1 cut(s) 478
Hin1II CATG 8 cut(s) 258, 336, 491, 627, 824, 838, 885, 1040
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HindIII AAGCTT 1 cut(s) 763
HinfI GANTC 8 cut(s) 107, 164, 370, 608, 614, 713, 751, 775
HpaII CCGG 1 cut(s) 478
HphI GGTGA 3 cut(s) 711, 793, 952
Hpy166II GTNNAC 5 cut(s) 163, 238, 485, 741, 826
Hpy188I TCNGA 6 cut(s) 65, 247, 300, 445, 613, 979
Hpy188III TCNNGA 2 cut(s) 512, 570
Hpy8I GTNNAC 5 cut(s) 163, 238, 485, 741, 826
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 6 cut(s) 80, 494, 509, 739, 887, 1015
HpyCH4III ACNGT 3 cut(s) 56, 313, 602
HpyCH4V TGCA 6 cut(s) 332, 627, 834, 887, 917, 1036
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 3 cut(s) 144, 318, 610
Hsp92II CATG 8 cut(s) 258, 336, 491, 627, 824, 838, 885, 1040
Kzo9I GATC 4 cut(s) 4, 508, 572, 979
LmnI GCTCC 1 cut(s) 518
MaeI CTAG 2 cut(s) 180, 1001
MaeIII GTNAC 5 cut(s) 89, 216, 394, 596, 958
MalI GATC 4 cut(s) 6, 510, 574, 981
MboI GATC 4 cut(s) 4, 508, 572, 979
MboII GAAGA 7 cut(s) 78, 313, 458, 667, 685, 861, 1004
MflI RGATCY 2 cut(s) 4, 572
MhlI GDGCHC 1 cut(s) 924
MluCI AATT 7 cut(s) 201, 248, 383, 400, 493, 583, 865
MlyI GAGTC 4 cut(s) 158, 602, 722, 784
Mph1103I ATGCAT 2 cut(s) 629, 836
MseI TTAA 8 cut(s) 140, 152, 533, 582, 761, 768, 863, 1045
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 1 cut(s) 478
MspR9I CCNGG 5 cut(s) 619, 648, 666, 687, 813
Mva1269I GAATGC 2 cut(s) 836, 1001
MvaI CCWGG 5 cut(s) 619, 648, 666, 687, 813
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeI CATATG 1 cut(s) 555
NdeII GATC 4 cut(s) 4, 508, 572, 979
NlaIII CATG 8 cut(s) 258, 336, 491, 627, 824, 838, 885, 1040
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 216, 958
NsiI ATGCAT 2 cut(s) 629, 836
NspI RCATGY 1 cut(s) 627
PaeI GCATGC 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PctI GAATGC 2 cut(s) 836, 1001
PfeI GAWTC 4 cut(s) 107, 370, 614, 751
PkrI GCNGC 3 cut(s) 287, 432, 459
PleI GAGTC 4 cut(s) 158, 602, 721, 783
PpsI GAGTC 4 cut(s) 158, 602, 721, 783
Psp6I CCWGG 5 cut(s) 617, 646, 664, 685, 811
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 5 cut(s) 617, 646, 664, 685, 811
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 794
PsuI RGATCY 2 cut(s) 4, 572
RsaI GTAC 5 cut(s) 315, 486, 560, 742, 810
RsaNI GTAC 5 cut(s) 314, 485, 559, 741, 809
SaqAI TTAA 8 cut(s) 140, 152, 533, 582, 761, 768, 863, 1045
SatI GCNGC 3 cut(s) 286, 431, 458
Sau3AI GATC 4 cut(s) 4, 508, 572, 979
Sau96I GGNCC 1 cut(s) 794
SchI GAGTC 4 cut(s) 158, 602, 722, 784
ScrFI CCNGG 5 cut(s) 619, 648, 666, 687, 813
SduI GDGCHC 1 cut(s) 924
SphI GCATGC 1 cut(s) 627
Sse9I AATT 7 cut(s) 201, 248, 383, 400, 493, 583, 865
SsiI CCGC 4 cut(s) 18, 286, 430, 457
SspI AATATT 2 cut(s) 125, 967
SspMI CTAG 2 cut(s) 180, 1001
StyD4I CCNGG 5 cut(s) 617, 646, 664, 685, 811
StyI CCWWGG 1 cut(s) 1000
TaaI ACNGT 3 cut(s) 56, 313, 602
TaqI TCGA 1 cut(s) 27
TasI AATT 7 cut(s) 201, 248, 383, 400, 493, 583, 865
TatI WGTACW 3 cut(s) 484, 558, 740
TauI GCSGC 3 cut(s) 288, 433, 460
TfiI GAWTC 4 cut(s) 107, 370, 614, 751
Tru1I TTAA 8 cut(s) 140, 152, 533, 582, 761, 768, 863, 1045
Tru9I TTAA 8 cut(s) 140, 152, 533, 582, 761, 768, 863, 1045
TscAI CASTG 1 cut(s) 316
TseFI GTSAC 2 cut(s) 216, 958
Tsp45I GTSAC 2 cut(s) 216, 958
TspDTI ATGAA 7 cut(s) 120, 213, 504, 651, 720, 934, 963
TspRI CASTG 1 cut(s) 316
XagI CCTNNNNNAGG 1 cut(s) 905
XapI RAATTY 4 cut(s) 248, 383, 493, 865
XceI RCATGY 1 cut(s) 627
XmaJI CCTAGG 1 cut(s) 1000
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 2 cut(s) 180, 1001
Zsp2I ATGCAT 2 cut(s) 629, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.