FvH4_6g48370

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013058
Physical Location & Seq
Forward (+)
36421189 .. 36424980
3792 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48370.t1

Sequence Viewer

Length: 927 bp
ATGGATCCGTCCGGCTCCGCTTCTTTCGACATATACGAGATTTATTGCCGATATTGTGATATTAGAAACGGAAATGGGTATGGGGAAGGTTATGGACAAATCGATGAATCACAGGTTGTCAGATATACAAAGGAGGCATTAACTCAGCTCTTAAATCTGGTGGAATCAAAACTTTCTGCTAGGGTATCAATCTTTGATGAAATTGGCAAGCTAATGTCACAGCTTGACTTGGTGGTAGACTTCTCAGAATTCTCGTGCTTCTATGAGTTCGTTTTTTTCTTATGTCGTGAAAGTGGTCAGAAGAGTATCACTGTTCCTATGGCCATTTCTGCATGGAGAATAGTCTTAGCTGGAAGGTTTCGCTTGCTGAATCAATGGTGTGAATTTGTTGAGAAAAATCAGCGGCATAACATCTCTGAAGATACTTGGAGGCAAGTTTTAGCTTTCAGCCGGTGTGTGCATGAAAATTTGGAAGGGTATGATCCTGAAGGAGCTTGGCCTGTCTTAATAGATGACTTCGTTGAGCATATGTACAGGTTTTCGGGATCTAATGTTAATTCTAACTTCTGTAACTGTGGCGACTCAGAATCCTGGTCATGCACAAATGATGAACTTCTACCTGGCTTGAAGAGTTATCCCGGTTTGAAGAGGAAAATACCGGGGGATGTTGGTCTTGATGAAATGGAGTCCTCACCTACCCCCTTCCCACAGAGTACAGACTTGAATCCTACATTAAGTTTTAAGAGGACTCGGGTGATGTCTTACGGGCCAGCAAATGATATACCTGGAAATCATGTAGACGAATGCATGCACGTTATTAGACATAGCAGTCCATTAAATTCTTCCAAATCTCCATGTGCAGTTGAAGGTTGCTTGTCAAAGGGCTTTGCGGAACTCTTACGGGAGAGGGGAGTTTCATTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

309

Amino Acids

34.82

Weight (kDa)

4.97

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 80 - 176 3.1e-30 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 828
AccI GTMKAC 2 cut(s) 237, 798
AciI CCGC 3 cut(s) 18, 403, 890
AclWI GGATC 3 cut(s) 12, 476, 553
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 4 cut(s) 248, 383, 466, 838
AcuI CTGAAG 2 cut(s) 438, 507
AfaI GTAC 2 cut(s) 533, 715
AgsI TTSAA 4 cut(s) 628, 646, 724, 866
AjnI CCWGG 3 cut(s) 590, 619, 784
AluBI AGCT 6 cut(s) 148, 211, 223, 350, 443, 494
AluI AGCT 6 cut(s) 148, 211, 223, 350, 443, 494
AlwI GGATC 3 cut(s) 12, 476, 553
Ama87I CYCGRG 1 cut(s) 750
AoxI GGCC 3 cut(s) 321, 497, 767
ApoI RAATTY 4 cut(s) 248, 383, 466, 838
AspS9I GGNCC 1 cut(s) 767
AsuC2I CCSGG 2 cut(s) 639, 660
AsuHPI GGTGA 2 cut(s) 684, 766
AvaI CYCGRG 1 cut(s) 750
BalI TGGCCA 1 cut(s) 323
BamHI GGATCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 253
BciT130I CCWGG 3 cut(s) 592, 621, 786
BcnI CCSGG 2 cut(s) 639, 660
BfaI CTAG 1 cut(s) 180
BisI GCNGC 1 cut(s) 404
BlsI GCNGC 1 cut(s) 405
Bme1390I CCNGG 5 cut(s) 592, 621, 639, 660, 786
BmeT110I CYCGRG 1 cut(s) 750
BmgT120I GGNCC 1 cut(s) 767
BmiI GGNNCC 2 cut(s) 6, 16
BmrFI CCNGG 5 cut(s) 592, 621, 639, 660, 786
BpuMI CCSGG 2 cut(s) 639, 660
Bsa29I ATCGAT 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 659
Bse118I RCCGGY 1 cut(s) 450
BseBI CCWGG 3 cut(s) 592, 621, 786
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 659
BseGI GGATG 1 cut(s) 670
BseMII CTCAG 3 cut(s) 158, 258, 597
BsgI GTGCAG 1 cut(s) 879
BshFI GGCC 3 cut(s) 323, 499, 769
BshVI ATCGAT 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 750
BsiSI CCGG 4 cut(s) 12, 451, 639, 659
BsmI GAATGC 1 cut(s) 809
BsnI GGCC 3 cut(s) 323, 499, 769
BsoBI CYCGRG 1 cut(s) 750
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 3 cut(s) 4, 481, 545
BspACI CCGC 3 cut(s) 18, 403, 890
BspANI GGCC 3 cut(s) 323, 499, 769
BspCNI CTCAG 3 cut(s) 157, 257, 596
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 6, 16
BspPI GGATC 3 cut(s) 12, 476, 553
BsrFI RCCGGY 1 cut(s) 450
BsrGI TGTACA 1 cut(s) 531
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 1 cut(s) 659
BssMI GATC 3 cut(s) 4, 481, 545
BssSI CACGAG 1 cut(s) 253
Bst2BI CACGAG 1 cut(s) 253
Bst2UI CCWGG 3 cut(s) 592, 621, 786
Bst4CI ACNGT 2 cut(s) 313, 575
Bst6I CTCTTC 3 cut(s) 296, 623, 641
BstAUI TGTACA 1 cut(s) 531
BstC8I GCNNGC 4 cut(s) 209, 365, 771, 809
BstDEI CTNAG 4 cut(s) 144, 244, 346, 583
BstF5I GGATG 1 cut(s) 670
BstKTI GATC 3 cut(s) 7, 484, 548
BstMBI GATC 3 cut(s) 4, 481, 545
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 3 cut(s) 592, 621, 786
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 5 cut(s) 590, 619, 637, 658, 784
BstX2I RGATCY 2 cut(s) 4, 545
BstYI RGATCY 2 cut(s) 4, 545
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 3 cut(s) 323, 499, 769
BsuTUI ATCGAT 1 cut(s) 102
BtsCI GGATG 1 cut(s) 670
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 4 cut(s) 209, 365, 771, 809
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 1 cut(s) 767
ClaI ATCGAT 1 cut(s) 102
Csp6I GTAC 2 cut(s) 532, 714
CviAII CATG 6 cut(s) 333, 461, 597, 794, 808, 855
CviQI GTAC 2 cut(s) 532, 714
DdeI CTNAG 4 cut(s) 144, 244, 346, 583
DpnI GATC 3 cut(s) 6, 483, 547
DpnII GATC 3 cut(s) 4, 481, 545
DrdI GACNNNNNNGTC 1 cut(s) 828
DseDI GACNNNNNNGTC 1 cut(s) 828
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 3 cut(s) 296, 623, 641
EarI CTCTTC 3 cut(s) 296, 623, 641
Eco57I CTGAAG 2 cut(s) 438, 507
Eco88I CYCGRG 1 cut(s) 750
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 3 cut(s) 590, 619, 784
EcoT22I ATGCAT 1 cut(s) 809
FaeI CATG 6 cut(s) 336, 464, 600, 797, 811, 858
FatI CATG 6 cut(s) 332, 460, 596, 793, 807, 854
FauNDI CATATG 1 cut(s) 528
FblI GTMKAC 2 cut(s) 237, 798
Fnu4HI GCNGC 1 cut(s) 404
FokI GGATG 1 cut(s) 677
Fsp4HI GCNGC 1 cut(s) 404
FspBI CTAG 1 cut(s) 180
GluI GCNGC 1 cut(s) 404
HaeIII GGCC 3 cut(s) 323, 499, 769
HapII CCGG 4 cut(s) 12, 451, 639, 659
Hin1II CATG 6 cut(s) 336, 464, 600, 797, 811, 858
HinfI GANTC 8 cut(s) 107, 164, 370, 581, 587, 686, 724, 748
HpaII CCGG 4 cut(s) 12, 451, 639, 659
HphI GGTGA 2 cut(s) 684, 766
Hpy166II GTNNAC 2 cut(s) 238, 799
Hpy188I TCNGA 5 cut(s) 122, 247, 300, 418, 586
Hpy188III TCNNGA 4 cut(s) 287, 485, 543, 674
Hpy8I GTNNAC 2 cut(s) 238, 799
HpyAV CCTTC 6 cut(s) 80, 348, 467, 482, 712, 860
HpyCH4III ACNGT 2 cut(s) 313, 575
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 6 cut(s) 332, 460, 600, 807, 811, 860
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 4 cut(s) 144, 244, 346, 583
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 6 cut(s) 336, 464, 600, 797, 811, 858
Kzo9I GATC 3 cut(s) 4, 481, 545
LmnI GCTCC 2 cut(s) 20, 491
MaeI CTAG 1 cut(s) 180
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 2 cut(s) 216, 569
MalI GATC 3 cut(s) 6, 483, 547
MboI GATC 3 cut(s) 4, 481, 545
MboII GAAGA 5 cut(s) 313, 431, 640, 658, 834
MflI RGATCY 2 cut(s) 4, 545
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 6 cut(s) 201, 248, 383, 466, 556, 838
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 3 cut(s) 575, 695, 742
MnlI CCTC 6 cut(s) 127, 423, 642, 700, 738, 900
Mox20I TGGCCA 1 cut(s) 323
Mph1103I ATGCAT 1 cut(s) 809
MscI TGGCCA 1 cut(s) 323
MseI TTAA 7 cut(s) 140, 152, 506, 555, 734, 741, 836
Msp20I TGGCCA 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 4 cut(s) 12, 451, 639, 659
MspR9I CCNGG 5 cut(s) 592, 621, 639, 660, 786
Mva1269I GAATGC 1 cut(s) 809
MvaI CCWGG 3 cut(s) 592, 621, 786
MwoI GCNNNNNNNGC 1 cut(s) 329
NciI CCSGG 2 cut(s) 639, 660
NdeI CATATG 1 cut(s) 528
NdeII GATC 3 cut(s) 4, 481, 545
NlaIII CATG 6 cut(s) 336, 464, 600, 797, 811, 858
NlaIV GGNNCC 2 cut(s) 6, 16
NmuCI GTSAC 1 cut(s) 216
NsiI ATGCAT 1 cut(s) 809
NspI RCATGY 1 cut(s) 811
PaeI GCATGC 1 cut(s) 811
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PctI GAATGC 1 cut(s) 809
PfeI GAWTC 5 cut(s) 107, 164, 370, 587, 724
PkrI GCNGC 1 cut(s) 405
PleI GAGTC 3 cut(s) 575, 694, 742
PpsI GAGTC 3 cut(s) 575, 694, 742
Psp6I CCWGG 3 cut(s) 590, 619, 784
PspGI CCWGG 3 cut(s) 590, 619, 784
PspN4I GGNNCC 2 cut(s) 6, 16
PspPI GGNCC 1 cut(s) 767
PsuI RGATCY 2 cut(s) 4, 545
RsaI GTAC 2 cut(s) 533, 715
RsaNI GTAC 2 cut(s) 532, 714
SaqAI TTAA 7 cut(s) 140, 152, 506, 555, 734, 741, 836
SatI GCNGC 1 cut(s) 404
Sau3AI GATC 3 cut(s) 4, 481, 545
Sau96I GGNCC 1 cut(s) 767
SchI GAGTC 3 cut(s) 575, 695, 742
ScrFI CCNGG 5 cut(s) 592, 621, 639, 660, 786
SphI GCATGC 1 cut(s) 811
Sse9I AATT 6 cut(s) 201, 248, 383, 466, 556, 838
SsiI CCGC 3 cut(s) 18, 403, 890
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 5 cut(s) 590, 619, 637, 658, 784
TaaI ACNGT 2 cut(s) 313, 575
TaiI ACGT 1 cut(s) 816
TaqI TCGA 2 cut(s) 27, 102
TasI AATT 6 cut(s) 201, 248, 383, 466, 556, 838
TatI WGTACW 2 cut(s) 531, 713
TauI GCSGC 1 cut(s) 406
TfiI GAWTC 5 cut(s) 107, 164, 370, 587, 724
Tru1I TTAA 7 cut(s) 140, 152, 506, 555, 734, 741, 836
Tru9I TTAA 7 cut(s) 140, 152, 506, 555, 734, 741, 836
TscAI CASTG 1 cut(s) 316
TseFI GTSAC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 6 cut(s) 120, 213, 477, 624, 693, 906
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 1 cut(s) 316
XapI RAATTY 4 cut(s) 248, 383, 466, 838
XceI RCATGY 1 cut(s) 811
XmiI GTMKAC 2 cut(s) 237, 798
XspI CTAG 1 cut(s) 180
Zsp2I ATGCAT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.