RLG00000021765

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013058
Physical Location & Seq
Forward (+)
78034829 .. 78038349
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021765

Sequence Viewer

Length: 927 bp
ATGGATCCGTCGGCCTCCGCTTCTTTCGACATATACGAGATTTATCGCCGATACTGTGATATCAGAAGAGGAAATGGGTATGGGGAAGGTTACAGACAAAACGATGAATTGCTGGTCGTCCAATATTCAAAGGAGGCATTAACTCAGCTCTTAAATCTGGTGGACTCAAAACTGCGTGCTAGGGTATCACTCTTTGATGAAATTAGCAAGCTAATGTCACAGCTTGACTTGGTGGTAGACTTTTCAGAATTCTCGCGCTTCTATGAGTTTGTTTTTTTCTTATGCCGCGAAAGTGGTCAGAAGAGTATCACCGTACCTAAGGCGATTTCTGCATGGAGAATAGTCTTAGCTGGGAGGTTTCGCTTGCTGAATCAATGGTGTGAATTTGTTGAGAAAAATCAGCGGCATAACATCTCTGAAGATACTTGGCGGCAAGTTTTAGCTTTTAGCCGGTGTGTACATGAAAATTTGGAAGGGTATGATCCTGAAGGAGCTTGGCCTGTCTTAATAGATGACTTCGTTGAGCATATGTACAGGTTTTCGGGATCTAATGTTAATTCTAACTTCTGTAACTGTGGCGACTCAGAATCCTGGGCATGCATATATGATGAACCTCTACCTGGCTTGAAGAGTTATCCTGGTTTGAAGAGGAAAATACCAGGGGATGTTGATATTGATGAAATGGAGTCCTCACCTACCTTCTTCCCAGAGTGTACAGACTTGAATCCTACTTTAAGCTTTAAGAGGAGTCGGGTGATGTCATACGGGCCAGCAAATGATATACCAGGAAATCATGTAGACGAATGCATGGAAGTTATTAGACACAGCAGTCCATTAAATTCTTCCAAATCTCCATGTGCAGTTGAAGGTTGCCTGTCAAAGGGCTTTGCAGGGCTCTTACGGGAGAGGGGAGTTTCATTTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

309

Amino Acids

35.19

Weight (kDa)

5.14

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 79 - 176 1.6e-30 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 828
AccI GTMKAC 2 cut(s) 237, 798
AccII CGCG 2 cut(s) 256, 288
AciI CCGC 4 cut(s) 18, 286, 403, 430
AclWI GGATC 3 cut(s) 12, 476, 553
AcsI RAATTY 4 cut(s) 248, 383, 466, 838
AcuI CTGAAG 2 cut(s) 438, 507
AfaI GTAC 4 cut(s) 315, 459, 533, 715
AfiI CCNNNNNNNGG 2 cut(s) 620, 880
AgsI TTSAA 5 cut(s) 129, 628, 646, 724, 866
AjnI CCWGG 5 cut(s) 590, 619, 637, 658, 784
AluBI AGCT 7 cut(s) 148, 211, 223, 350, 443, 494, 738
AluI AGCT 7 cut(s) 148, 211, 223, 350, 443, 494, 738
AlwI GGATC 3 cut(s) 12, 476, 553
AoxI GGCC 3 cut(s) 12, 497, 767
ApoI RAATTY 4 cut(s) 248, 383, 466, 838
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 767
AsuHPI GGTGA 3 cut(s) 301, 684, 766
AxyI CCTNAGG 1 cut(s) 318
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 897
BciT130I CCWGG 5 cut(s) 592, 621, 639, 660, 786
BfaI CTAG 1 cut(s) 180
BisI GCNGC 3 cut(s) 286, 404, 431
BlsI GCNGC 3 cut(s) 287, 405, 432
Bme1390I CCNGG 5 cut(s) 592, 621, 639, 660, 786
BmgT120I GGNCC 1 cut(s) 767
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 5 cut(s) 592, 621, 639, 660, 786
BsaJI CCNNGG 2 cut(s) 591, 659
Bsc4I CCNNNNNNNGG 2 cut(s) 620, 880
Bse118I RCCGGY 1 cut(s) 450
Bse21I CCTNAGG 1 cut(s) 318
BseBI CCWGG 5 cut(s) 592, 621, 639, 660, 786
BseDI CCNNGG 2 cut(s) 591, 659
BseGI GGATG 1 cut(s) 670
BseLI CCNNNNNNNGG 2 cut(s) 620, 880
BseMII CTCAG 2 cut(s) 158, 597
BseRI GAGGAG 1 cut(s) 760
BseYI CCCAGC 1 cut(s) 350
BsgI GTGCAG 1 cut(s) 879
Bsh1236I CGCG 2 cut(s) 256, 288
BshFI GGCC 3 cut(s) 14, 499, 769
BsiSI CCGG 1 cut(s) 451
BslI CCNNNNNNNGG 2 cut(s) 620, 880
BsmI GAATGC 1 cut(s) 809
BsnI GGCC 3 cut(s) 14, 499, 769
Bsp1286I GDGCHC 1 cut(s) 897
Bsp1407I TGTACA 3 cut(s) 457, 531, 713
Bsp143I GATC 3 cut(s) 4, 481, 545
BspACI CCGC 4 cut(s) 18, 286, 403, 430
BspANI GGCC 3 cut(s) 14, 499, 769
BspCNI CTCAG 2 cut(s) 157, 596
BspFNI CGCG 2 cut(s) 256, 288
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 3 cut(s) 12, 476, 553
BsrFI RCCGGY 1 cut(s) 450
BsrGI TGTACA 3 cut(s) 457, 531, 713
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 2 cut(s) 591, 659
BssMI GATC 3 cut(s) 4, 481, 545
Bst2UI CCWGG 5 cut(s) 592, 621, 639, 660, 786
Bst4CI ACNGT 3 cut(s) 56, 313, 575
Bst6I CTCTTC 4 cut(s) 61, 296, 623, 641
BstAUI TGTACA 3 cut(s) 457, 531, 713
BstC8I GCNNGC 5 cut(s) 177, 209, 365, 598, 771
BstDEI CTNAG 4 cut(s) 144, 318, 346, 583
BstENI CCTNNNNNAGG 1 cut(s) 878
BstF5I GGATG 1 cut(s) 670
BstFNI CGCG 2 cut(s) 256, 288
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 3 cut(s) 7, 484, 548
BstMBI GATC 3 cut(s) 4, 481, 545
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 5 cut(s) 592, 621, 639, 660, 786
BstNSI RCATGY 1 cut(s) 600
BstSCI CCNGG 5 cut(s) 590, 619, 637, 658, 784
BstUI CGCG 2 cut(s) 256, 288
BstX2I RGATCY 2 cut(s) 4, 545
BstYI RGATCY 2 cut(s) 4, 545
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 3 cut(s) 14, 499, 769
BtsCI GGATG 1 cut(s) 670
Cac8I GCNNGC 5 cut(s) 177, 209, 365, 598, 771
CfoI GCGC 1 cut(s) 258
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 1 cut(s) 767
Csp6I GTAC 4 cut(s) 314, 458, 532, 714
CviAII CATG 6 cut(s) 333, 461, 597, 794, 808, 855
CviQI GTAC 4 cut(s) 314, 458, 532, 714
DdeI CTNAG 4 cut(s) 144, 318, 346, 583
DpnI GATC 3 cut(s) 6, 483, 547
DpnII GATC 3 cut(s) 4, 481, 545
DrdI GACNNNNNNGTC 1 cut(s) 828
DseDI GACNNNNNNGTC 1 cut(s) 828
Eam1104I CTCTTC 4 cut(s) 61, 296, 623, 641
EarI CTCTTC 4 cut(s) 61, 296, 623, 641
Eco24I GRGCYC 1 cut(s) 897
Eco32I GATATC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 438, 507
Eco81I CCTNAGG 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 878
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 5 cut(s) 590, 619, 637, 658, 784
EcoRV GATATC 1 cut(s) 61
EcoT22I ATGCAT 2 cut(s) 602, 809
EcoT38I GRGCYC 1 cut(s) 897
FaeI CATG 6 cut(s) 336, 464, 600, 797, 811, 858
FatI CATG 6 cut(s) 332, 460, 596, 793, 807, 854
FauNDI CATATG 1 cut(s) 528
FblI GTMKAC 2 cut(s) 237, 798
Fnu4HI GCNGC 3 cut(s) 286, 404, 431
FokI GGATG 1 cut(s) 677
FriOI GRGCYC 1 cut(s) 897
Fsp4HI GCNGC 3 cut(s) 286, 404, 431
FspBI CTAG 1 cut(s) 180
GlaI GCGC 1 cut(s) 257
GluI GCNGC 3 cut(s) 286, 404, 431
GsaI CCCAGC 1 cut(s) 354
HaeIII GGCC 3 cut(s) 14, 499, 769
HapII CCGG 1 cut(s) 451
HhaI GCGC 1 cut(s) 258
Hin1II CATG 6 cut(s) 336, 464, 600, 797, 811, 858
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 736
HinfI GANTC 7 cut(s) 164, 370, 581, 587, 686, 724, 748
HpaII CCGG 1 cut(s) 451
HphI GGTGA 3 cut(s) 301, 684, 766
Hpy166II GTNNAC 5 cut(s) 163, 238, 458, 714, 799
Hpy188I TCNGA 5 cut(s) 65, 247, 300, 418, 586
Hpy188III TCNNGA 2 cut(s) 485, 543
Hpy8I GTNNAC 5 cut(s) 163, 238, 458, 714, 799
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 5 cut(s) 80, 467, 482, 709, 860
HpyCH4III ACNGT 3 cut(s) 56, 313, 575
HpyCH4V TGCA 5 cut(s) 332, 600, 807, 860, 890
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 4 cut(s) 144, 318, 346, 583
Hsp92II CATG 6 cut(s) 336, 464, 600, 797, 811, 858
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 3 cut(s) 4, 481, 545
LmnI GCTCC 1 cut(s) 491
MaeI CTAG 1 cut(s) 180
MaeIII GTNAC 3 cut(s) 89, 216, 569
MalI GATC 3 cut(s) 6, 483, 547
MboI GATC 3 cut(s) 4, 481, 545
MboII GAAGA 7 cut(s) 78, 313, 431, 640, 658, 694, 834
MflI RGATCY 2 cut(s) 4, 545
MhlI GDGCHC 1 cut(s) 897
MluCI AATT 7 cut(s) 107, 201, 248, 383, 466, 556, 838
MlyI GAGTC 4 cut(s) 158, 575, 695, 757
MnlI CCTC 9 cut(s) 25, 62, 127, 348, 624, 642, 700, 738, 900
Mph1103I ATGCAT 2 cut(s) 602, 809
MseI TTAA 7 cut(s) 140, 152, 506, 555, 734, 741, 836
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 5 cut(s) 592, 621, 639, 660, 786
Mva1269I GAATGC 1 cut(s) 809
MvaI CCWGG 5 cut(s) 592, 621, 639, 660, 786
MvnI CGCG 2 cut(s) 256, 288
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeI CATATG 1 cut(s) 528
NdeII GATC 3 cut(s) 4, 481, 545
NlaIII CATG 6 cut(s) 336, 464, 600, 797, 811, 858
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 216
NsiI ATGCAT 2 cut(s) 602, 809
NspI RCATGY 1 cut(s) 600
PaeI GCATGC 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PctI GAATGC 1 cut(s) 809
PfeI GAWTC 3 cut(s) 370, 587, 724
PkrI GCNGC 3 cut(s) 287, 405, 432
PleI GAGTC 4 cut(s) 158, 575, 694, 756
PpsI GAGTC 4 cut(s) 158, 575, 694, 756
Psp6I CCWGG 5 cut(s) 590, 619, 637, 658, 784
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 5 cut(s) 590, 619, 637, 658, 784
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 767
PsuI RGATCY 2 cut(s) 4, 545
RsaI GTAC 4 cut(s) 315, 459, 533, 715
RsaNI GTAC 4 cut(s) 314, 458, 532, 714
SaqAI TTAA 7 cut(s) 140, 152, 506, 555, 734, 741, 836
SatI GCNGC 3 cut(s) 286, 404, 431
Sau3AI GATC 3 cut(s) 4, 481, 545
Sau96I GGNCC 1 cut(s) 767
SchI GAGTC 4 cut(s) 158, 575, 695, 757
ScrFI CCNGG 5 cut(s) 592, 621, 639, 660, 786
SduI GDGCHC 1 cut(s) 897
SphI GCATGC 1 cut(s) 600
Sse9I AATT 7 cut(s) 107, 201, 248, 383, 466, 556, 838
SsiI CCGC 4 cut(s) 18, 286, 403, 430
SspI AATATT 1 cut(s) 125
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 5 cut(s) 590, 619, 637, 658, 784
TaaI ACNGT 3 cut(s) 56, 313, 575
TaqI TCGA 1 cut(s) 27
TasI AATT 7 cut(s) 107, 201, 248, 383, 466, 556, 838
TatI WGTACW 3 cut(s) 457, 531, 713
TauI GCSGC 3 cut(s) 288, 406, 433
TfiI GAWTC 3 cut(s) 370, 587, 724
Tru1I TTAA 7 cut(s) 140, 152, 506, 555, 734, 741, 836
Tru9I TTAA 7 cut(s) 140, 152, 506, 555, 734, 741, 836
TseFI GTSAC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 6 cut(s) 120, 213, 477, 624, 693, 906
XagI CCTNNNNNAGG 1 cut(s) 878
XapI RAATTY 4 cut(s) 248, 383, 466, 838
XceI RCATGY 1 cut(s) 600
XmiI GTMKAC 2 cut(s) 237, 798
XspI CTAG 1 cut(s) 180
Zsp2I ATGCAT 2 cut(s) 602, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.