Rh2DG625300

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013058
Physical Location & Seq
Forward (+)
85481274 .. 85485189
3916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG625300.1

Sequence Viewer

Length: 783 bp
ATGAGTTTGTGTTTTTCTTATGCCGCGAAAGTGGTCAGAAGAGTATCAGTAAGTTTTATTTTTTTATTTTTTTGTTCCAGGCAGTATAATAATTCTCAATCTCTTTTATTAATTTCCAACAATAGCCATATGAAAATTCATTTTCCTTTATTTGAGTTATTTGCAGCTGTACCTAAGGCGATTTCTGCATGGAGAATAGTCTTAGCTGGGAGGTTTCGCTTGCTGAATCAATGGTGTGAATTTGTTGAGAAAAATCAGCGGCATAACATCTCTGAAGATACTTGGCGGCAAGTTTTAGCTTTTAGTCGGTGTGTACATGAAAATTTGGAAGGGTATGATCCTGAAGGAGCTTGGCCTGTCTTAATAGATGACTTCGTTGAGCATATGTACAGGTTTTCGGGATCTAATGTTAATTCTAACTTTTGTAACTGTGGCGACTCAGAATCCTGGTCATGCATATATGATGAACCTCTACCTGGCTTGAAGAGTTATCCTGGTTTGAAGAGGAAAATACCAGGGGATGTTGATATCGATGAAATGGAGTCCTCACCTACCTCCTTCCCAGAGTGTGCAGACTTGAATCCTACATTAAGCTTTAAGAGGAGTCGGGTGATGTCATACGGGCCAGCGAATGATATAGCAGGAAATCATGTAGACGAATGCATGGAAGTTATTAGACATAGCAGTCCATTAAATTCTTCCAAACCTCCGTGTGCAGTTGAAGGTTGCCTGTCAAAGGGGTTTGCAGGGCTCTTACGGGAGAGGGGAGTTTCATTTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

260

Amino Acids

29.43

Weight (kDa)

6.17

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 58 - 128 6.2e-21 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 684
AccI GTMKAC 1 cut(s) 654
AccII CGCG 1 cut(s) 26
AciI CCGC 3 cut(s) 24, 259, 286
AclWI GGATC 2 cut(s) 332, 409
AcsI RAATTY 4 cut(s) 135, 239, 322, 694
AcuI CTGAAG 2 cut(s) 294, 363
AfaI GTAC 3 cut(s) 171, 315, 389
AfiI CCNNNNNNNGG 2 cut(s) 476, 736
AgsI TTSAA 4 cut(s) 484, 502, 580, 722
AjnI CCWGG 5 cut(s) 77, 446, 475, 493, 514
AluBI AGCT 5 cut(s) 167, 206, 299, 350, 594
AluI AGCT 5 cut(s) 167, 206, 299, 350, 594
AlwI GGATC 2 cut(s) 332, 409
AoxI GGCC 2 cut(s) 353, 623
ApeKI GCWGC 1 cut(s) 164
ApoI RAATTY 4 cut(s) 135, 239, 322, 694
AseI ATTAAT 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 623
AsuHPI GGTGA 2 cut(s) 540, 622
AxyI CCTNAGG 1 cut(s) 174
BanII GRGCYC 1 cut(s) 753
BbvI GCAGC 1 cut(s) 176
BciT130I CCWGG 5 cut(s) 79, 448, 477, 495, 516
BisI GCNGC 4 cut(s) 24, 165, 260, 287
BlsI GCNGC 4 cut(s) 25, 166, 261, 288
Bme1390I CCNGG 5 cut(s) 79, 448, 477, 495, 516
BmgT120I GGNCC 1 cut(s) 623
BmrFI CCNGG 5 cut(s) 79, 448, 477, 495, 516
Bsa29I ATCGAT 1 cut(s) 531
BsaJI CCNNGG 1 cut(s) 515
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 736
Bse21I CCTNAGG 1 cut(s) 174
BseBI CCWGG 5 cut(s) 79, 448, 477, 495, 516
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 515
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 2 cut(s) 476, 736
BseMII CTCAG 1 cut(s) 453
BseRI GAGGAG 1 cut(s) 616
BseXI GCAGC 1 cut(s) 176
BseYI CCCAGC 1 cut(s) 206
BsgI GTGCAG 2 cut(s) 591, 735
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 2 cut(s) 355, 625
BshVI ATCGAT 1 cut(s) 531
BslI CCNNNNNNNGG 2 cut(s) 476, 736
BsmI GAATGC 1 cut(s) 665
BsnI GGCC 2 cut(s) 355, 625
Bsp1286I GDGCHC 1 cut(s) 753
Bsp1407I TGTACA 2 cut(s) 313, 387
Bsp143I GATC 2 cut(s) 337, 401
BspACI CCGC 3 cut(s) 24, 259, 286
BspANI GGCC 2 cut(s) 355, 625
BspCNI CTCAG 1 cut(s) 452
BspDI ATCGAT 1 cut(s) 531
BspFNI CGCG 1 cut(s) 26
BspPI GGATC 2 cut(s) 332, 409
BsrGI TGTACA 2 cut(s) 313, 387
BssECI CCNNGG 1 cut(s) 515
BssMI GATC 2 cut(s) 337, 401
Bst2UI CCWGG 5 cut(s) 79, 448, 477, 495, 516
Bst4CI ACNGT 1 cut(s) 431
Bst6I CTCTTC 3 cut(s) 34, 479, 497
BstAUI TGTACA 2 cut(s) 313, 387
BstC8I GCNNGC 2 cut(s) 221, 627
BstDEI CTNAG 3 cut(s) 174, 202, 439
BstENI CCTNNNNNAGG 1 cut(s) 734
BstF5I GGATG 1 cut(s) 526
BstFNI CGCG 1 cut(s) 26
BstKTI GATC 2 cut(s) 340, 404
BstMBI GATC 2 cut(s) 337, 401
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 5 cut(s) 79, 448, 477, 495, 516
BstSCI CCNGG 5 cut(s) 77, 446, 475, 493, 514
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 1 cut(s) 176
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
Bsu15I ATCGAT 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 174
BsuRI GGCC 2 cut(s) 355, 625
BsuTUI ATCGAT 1 cut(s) 531
BtsCI GGATG 1 cut(s) 526
Cac8I GCNNGC 2 cut(s) 221, 627
Cfr13I GGNCC 1 cut(s) 623
ClaI ATCGAT 1 cut(s) 531
Csp6I GTAC 3 cut(s) 170, 314, 388
CviAII CATG 5 cut(s) 189, 317, 453, 650, 664
CviQI GTAC 3 cut(s) 170, 314, 388
DdeI CTNAG 3 cut(s) 174, 202, 439
DpnI GATC 2 cut(s) 339, 403
DpnII GATC 2 cut(s) 337, 401
DrdI GACNNNNNNGTC 1 cut(s) 684
DseDI GACNNNNNNGTC 1 cut(s) 684
Eam1104I CTCTTC 3 cut(s) 34, 479, 497
EarI CTCTTC 3 cut(s) 34, 479, 497
Eco24I GRGCYC 1 cut(s) 753
Eco32I GATATC 1 cut(s) 529
Eco57I CTGAAG 2 cut(s) 294, 363
Eco81I CCTNAGG 1 cut(s) 174
EcoNI CCTNNNNNAGG 1 cut(s) 734
EcoRII CCWGG 5 cut(s) 77, 446, 475, 493, 514
EcoRV GATATC 1 cut(s) 529
EcoT22I ATGCAT 2 cut(s) 458, 665
EcoT38I GRGCYC 1 cut(s) 753
FaeI CATG 5 cut(s) 192, 320, 456, 653, 667
FatI CATG 5 cut(s) 188, 316, 452, 649, 663
FauNDI CATATG 2 cut(s) 129, 384
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 4 cut(s) 24, 165, 260, 287
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 753
Fsp4HI GCNGC 4 cut(s) 24, 165, 260, 287
GluI GCNGC 4 cut(s) 24, 165, 260, 287
GsaI CCCAGC 1 cut(s) 210
HaeIII GGCC 2 cut(s) 355, 625
Hin1II CATG 5 cut(s) 192, 320, 456, 653, 667
HindIII AAGCTT 1 cut(s) 592
HinfI GANTC 6 cut(s) 226, 437, 443, 542, 580, 604
HphI GGTGA 2 cut(s) 540, 622
Hpy166II GTNNAC 2 cut(s) 314, 655
Hpy188I TCNGA 3 cut(s) 38, 274, 442
Hpy188III TCNNGA 2 cut(s) 341, 399
Hpy8I GTNNAC 2 cut(s) 314, 655
HpyAV CCTTC 4 cut(s) 323, 338, 568, 716
HpyCH4III ACNGT 1 cut(s) 431
HpyCH4V TGCA 7 cut(s) 164, 188, 456, 572, 663, 716, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 3 cut(s) 174, 202, 439
Hsp92II CATG 5 cut(s) 192, 320, 456, 653, 667
Kzo9I GATC 2 cut(s) 337, 401
LmnI GCTCC 1 cut(s) 347
Lsp1109I GCAGC 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 425
MalI GATC 2 cut(s) 339, 403
MboI GATC 2 cut(s) 337, 401
MboII GAAGA 5 cut(s) 51, 287, 496, 514, 690
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 1 cut(s) 753
MluCI AATT 7 cut(s) 91, 111, 135, 239, 322, 412, 694
MlyI GAGTC 3 cut(s) 431, 551, 613
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 8 cut(s) 204, 480, 498, 556, 565, 594, 717, 756
Mph1103I ATGCAT 2 cut(s) 458, 665
MseI TTAA 6 cut(s) 110, 362, 411, 590, 597, 692
MspA1I CMGCKG 2 cut(s) 167, 259
MspR9I CCNGG 5 cut(s) 79, 448, 477, 495, 516
Mva1269I GAATGC 1 cut(s) 665
MvaI CCWGG 5 cut(s) 79, 448, 477, 495, 516
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 1 cut(s) 185
NdeI CATATG 2 cut(s) 129, 384
NdeII GATC 2 cut(s) 337, 401
NlaIII CATG 5 cut(s) 192, 320, 456, 653, 667
NsiI ATGCAT 2 cut(s) 458, 665
PctI GAATGC 1 cut(s) 665
PfeI GAWTC 3 cut(s) 226, 443, 580
PkrI GCNGC 4 cut(s) 25, 166, 261, 288
PleI GAGTC 3 cut(s) 431, 550, 612
PpsI GAGTC 3 cut(s) 431, 550, 612
PshBI ATTAAT 1 cut(s) 110
Psp6I CCWGG 5 cut(s) 77, 446, 475, 493, 514
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 5 cut(s) 77, 446, 475, 493, 514
PspPI GGNCC 1 cut(s) 623
PsuI RGATCY 1 cut(s) 401
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 3 cut(s) 171, 315, 389
RsaNI GTAC 3 cut(s) 170, 314, 388
SaqAI TTAA 6 cut(s) 110, 362, 411, 590, 597, 692
SatI GCNGC 4 cut(s) 24, 165, 260, 287
Sau3AI GATC 2 cut(s) 337, 401
Sau96I GGNCC 1 cut(s) 623
SchI GAGTC 3 cut(s) 431, 551, 613
ScrFI CCNGG 5 cut(s) 79, 448, 477, 495, 516
SduI GDGCHC 1 cut(s) 753
Sse9I AATT 7 cut(s) 91, 111, 135, 239, 322, 412, 694
SsiI CCGC 3 cut(s) 24, 259, 286
StyD4I CCNGG 5 cut(s) 77, 446, 475, 493, 514
TaaI ACNGT 1 cut(s) 431
TaqI TCGA 1 cut(s) 531
TasI AATT 7 cut(s) 91, 111, 135, 239, 322, 412, 694
TatI WGTACW 2 cut(s) 313, 387
TauI GCSGC 3 cut(s) 26, 262, 289
TfiI GAWTC 3 cut(s) 226, 443, 580
Tru1I TTAA 6 cut(s) 110, 362, 411, 590, 597, 692
Tru9I TTAA 6 cut(s) 110, 362, 411, 590, 597, 692
TseI GCWGC 1 cut(s) 164
TspDTI ATGAA 6 cut(s) 128, 146, 333, 480, 549, 762
TspGWI ACGGA 1 cut(s) 699
VspI ATTAAT 1 cut(s) 110
XagI CCTNNNNNAGG 1 cut(s) 734
XapI RAATTY 4 cut(s) 135, 239, 322, 694
XmiI GTMKAC 1 cut(s) 654
Zsp2I ATGCAT 2 cut(s) 458, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.