MD09G1052100.v1.1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013058
Physical Location & Seq
Reverse (-)
3476409 .. 3479500
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1052100.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGATCCGTCCGGGTCCACTCGATTCGACATCTTTGAGATTTATCGCCGATTCTGCGATATCAGGACGGGAAATGGATACGGTCACGGTGAAGGATACAGAGAAAATGATGATTCACAGATGGCTAAATATTCAAGGGAGGCATTAACTCAGCTCTTATATCTGGTGGAATCAAAACTGCACGCTAGGATATCAATTTTTGATGAAGTTTTCAAGCTAATGTTGCGGTTGGATTCGATGGTAGACTTTTCTGAATTCTCACGTTTCTATGATTTTGTTTTCTTCATGTGCCGTGAAAACGGTCAGAAGAATATCACTGTAAATAAGGCGGTTAATGCATGGAGAGTAGTCCTAGCTGGGAGGTTTCGGTTGCTGAATCAATGGTGTGATTTTGTTGAGAAAAATCAGCGGCATAACATCTCTGAAGATACCTGGCGGCAAGTTTTAGCTTTTAGCCGCTGTGTGCATGAAAATCTGGAAGGGTATGATCCTGAAGGAGCTTGGCCTGTTTTAATAGATGACTTTGTTGAACATATGTACAGGGTTTCCGGATCTAACAATAACTCTAGCTTCAACTGTAACTGTGGTGATGCAGAATCGTGGTCATGTACATATGATGACCCTCTTCCTGGCTTGAAGATTTTACCTGGTTTGAAAAGGAAGTTACCTGGGGAGGACCTTCACATGGAAGAAGTGGTGCCCTCGCCCGCCCTCTTCCCATACTGTACAGACTGGAATCCTGCATTAAGCACTAAGAAAAGTCGGCCAATGTCTTACATACCAGCAAACTGGGAGGAGAGTACACCAGGAAATACTGTGGATGATTCCATGGAAACCATAAGACATAGCAGTCCAGTGAATTCTTCCAAATCTCCATGCGCGGTTGAAGGGTGTCTGTTGAAGGGTTTTGCAGGACTGCTTTCTGCGCGGTCCTGTCTGCAATTTGATCGGGACAGGGGAGTTTCATTTTCAAAGAGTCACAAATATCGAAAGCGTCTTTTACGAGGAGATGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

340

Amino Acids

38.9

Weight (kDa)

6.1

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 82 - 178 3.4e-30 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 849
AccB1I GGYRCC 1 cut(s) 697
AccI GTMKAC 1 cut(s) 243
AccII CGCG 2 cut(s) 881, 928
AccIII TCCGGA 1 cut(s) 548
AciI CCGC 8 cut(s) 226, 329, 409, 436, 457, 708, 881, 928
AclWI GGATC 3 cut(s) 12, 482, 559
AcoI YGGCCR 1 cut(s) 764
AcsI RAATTY 2 cut(s) 254, 859
AcuI CTGAAG 2 cut(s) 444, 513
AfaI GTAC 4 cut(s) 539, 610, 727, 802
AfiI CCNNNNNNNGG 2 cut(s) 629, 685
AgsI TTSAA 9 cut(s) 135, 214, 530, 574, 637, 655, 887, 901, 972
AjnI CCWGG 5 cut(s) 431, 628, 646, 667, 805
AluBI AGCT 6 cut(s) 154, 217, 356, 449, 500, 570
AluI AGCT 6 cut(s) 154, 217, 356, 449, 500, 570
AlwI GGATC 3 cut(s) 12, 482, 559
Aor13HI TCCGGA 1 cut(s) 548
AoxI GGCC 2 cut(s) 503, 764
ApoI RAATTY 2 cut(s) 254, 859
AspLEI GCGC 2 cut(s) 881, 928
AspS9I GGNCC 3 cut(s) 15, 676, 930
AsuC2I CCSGG 1 cut(s) 13
AsuHPI GGTGA 2 cut(s) 101, 599
AvaII GGWCC 3 cut(s) 15, 676, 930
BaeGI GKGCMC 1 cut(s) 702
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 697
BccI CCATC 2 cut(s) 115, 232
BceAI ACGGC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 929, 963
BciT130I CCWGG 5 cut(s) 433, 630, 648, 669, 807
BciVI GTATCC 2 cut(s) 71, 89
BcnI CCSGG 1 cut(s) 13
BfaI CTAG 3 cut(s) 186, 353, 567
BfuI GTATCC 2 cut(s) 71, 89
BisI GCNGC 3 cut(s) 410, 437, 457
BlsI GCNGC 3 cut(s) 411, 438, 458
Bme1390I CCNGG 6 cut(s) 13, 433, 630, 648, 669, 807
Bme18I GGWCC 3 cut(s) 15, 676, 930
BmgT120I GGNCC 3 cut(s) 15, 676, 930
BmiI GGNNCC 3 cut(s) 6, 16, 699
BmrFI CCNGG 6 cut(s) 13, 433, 630, 648, 669, 807
BmrI ACTGGG 1 cut(s) 799
BmsI GCATC 2 cut(s) 580, 1000
BmuI ACTGGG 1 cut(s) 799
BpuMI CCSGG 1 cut(s) 13
BsaJI CCNNGG 2 cut(s) 668, 828
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 1 cut(s) 999
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 685
Bse1I ACTGG 3 cut(s) 737, 794, 854
BseAI TCCGGA 1 cut(s) 548
BseBI CCWGG 5 cut(s) 433, 630, 648, 669, 807
BseDI CCNNGG 2 cut(s) 668, 828
BseGI GGATG 1 cut(s) 826
BseLI CCNNNNNNNGG 2 cut(s) 629, 685
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 3 cut(s) 737, 794, 854
BseRI GAGGAG 2 cut(s) 809, 1020
BseSI GKGCMC 1 cut(s) 702
BseYI CCCAGC 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 164
Bsh1236I CGCG 2 cut(s) 881, 928
BshFI GGCC 2 cut(s) 505, 766
BshNI GGYRCC 1 cut(s) 697
BsiSI CCGG 2 cut(s) 12, 549
BslFI GGGAC 1 cut(s) 965
BslI CCNNNNNNNGG 2 cut(s) 629, 685
BsmFI GGGAC 1 cut(s) 965
BsnI GGCC 2 cut(s) 505, 766
Bsp1286I GDGCHC 1 cut(s) 702
Bsp13I TCCGGA 1 cut(s) 548
Bsp1407I TGTACA 3 cut(s) 537, 608, 725
Bsp143I GATC 4 cut(s) 4, 487, 551, 946
Bsp19I CCATGG 1 cut(s) 828
BspACI CCGC 8 cut(s) 226, 329, 409, 436, 457, 708, 881, 928
BspANI GGCC 2 cut(s) 505, 766
BspCNI CTCAG 1 cut(s) 163
BspEI TCCGGA 1 cut(s) 548
BspFNI CGCG 2 cut(s) 881, 928
BspLI GGNNCC 3 cut(s) 6, 16, 699
BspPI GGATC 3 cut(s) 12, 482, 559
BspT107I GGYRCC 1 cut(s) 697
BsrGI TGTACA 3 cut(s) 537, 608, 725
BsrI ACTGG 3 cut(s) 737, 794, 854
BssECI CCNNGG 2 cut(s) 668, 828
BssMI GATC 4 cut(s) 4, 487, 551, 946
BssT1I CCWWGG 1 cut(s) 828
Bst2UI CCWGG 5 cut(s) 433, 630, 648, 669, 807
Bst4CI ACNGT 8 cut(s) 83, 89, 302, 319, 578, 584, 725, 817
Bst6I CTCTTC 2 cut(s) 630, 719
BstAUI TGTACA 3 cut(s) 537, 608, 725
BstC8I GCNNGC 2 cut(s) 183, 708
BstDEI CTNAG 2 cut(s) 150, 753
BstDSI CCRYGG 1 cut(s) 828
BstF5I GGATG 1 cut(s) 826
BstFNI CGCG 2 cut(s) 881, 928
BstHHI GCGC 2 cut(s) 881, 928
BstKTI GATC 4 cut(s) 7, 490, 554, 949
BstMBI GATC 4 cut(s) 4, 487, 551, 946
BstMWI GCNNNNNNNGC 4 cut(s) 54, 223, 335, 925
BstNI CCWGG 5 cut(s) 433, 630, 648, 669, 807
BstSCI CCNGG 6 cut(s) 11, 431, 628, 646, 667, 805
BstSLI GKGCMC 1 cut(s) 702
BstUI CGCG 2 cut(s) 881, 928
BstX2I RGATCY 2 cut(s) 4, 551
BstXI CCANNNNNNTGG 1 cut(s) 789
BstYI RGATCY 2 cut(s) 4, 551
BsuI GTATCC 2 cut(s) 71, 89
BsuRI GGCC 2 cut(s) 505, 766
BtgI CCRYGG 1 cut(s) 828
BtsCI GGATG 1 cut(s) 826
BtsIMutI CAGTG 2 cut(s) 315, 861
Cac8I GCNNGC 2 cut(s) 183, 708
CfoI GCGC 2 cut(s) 881, 928
Cfr13I GGNCC 3 cut(s) 15, 676, 930
CseI GACGC 1 cut(s) 983
CsiI ACCWGGT 1 cut(s) 646
Csp6I GTAC 4 cut(s) 538, 609, 726, 801
CviAII CATG 7 cut(s) 286, 339, 467, 606, 685, 829, 876
CviQI GTAC 4 cut(s) 538, 609, 726, 801
DdeI CTNAG 2 cut(s) 150, 753
DpnI GATC 4 cut(s) 6, 489, 553, 948
DpnII GATC 4 cut(s) 4, 487, 551, 946
DrdI GACNNNNNNGTC 1 cut(s) 849
DseDI GACNNNNNNGTC 1 cut(s) 849
EaeI YGGCCR 1 cut(s) 764
Eam1104I CTCTTC 2 cut(s) 630, 719
EarI CTCTTC 2 cut(s) 630, 719
Eco130I CCWWGG 1 cut(s) 828
Eco32I GATATC 2 cut(s) 61, 192
Eco47I GGWCC 3 cut(s) 15, 676, 930
Eco57I CTGAAG 2 cut(s) 444, 513
EcoO109I RGGNCCY 1 cut(s) 676
EcoRI GAATTC 2 cut(s) 254, 859
EcoRII CCWGG 5 cut(s) 431, 628, 646, 667, 805
EcoRV GATATC 2 cut(s) 61, 192
EcoT14I CCWWGG 1 cut(s) 828
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 828
FaeI CATG 7 cut(s) 289, 342, 470, 609, 688, 832, 879
FaqI GGGAC 1 cut(s) 965
FatI CATG 7 cut(s) 285, 338, 466, 605, 684, 828, 875
FauI CCCGC 1 cut(s) 715
FauNDI CATATG 2 cut(s) 534, 613
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 410, 437, 457
FokI GGATG 1 cut(s) 833
Fsp4HI GCNGC 3 cut(s) 410, 437, 457
FspBI CTAG 3 cut(s) 186, 353, 567
GlaI GCGC 2 cut(s) 880, 927
GluI GCNGC 3 cut(s) 410, 437, 457
GsaI CCCAGC 1 cut(s) 360
HaeIII GGCC 2 cut(s) 505, 766
HapII CCGG 2 cut(s) 12, 549
HgaI GACGC 1 cut(s) 983
HhaI GCGC 2 cut(s) 881, 928
Hin1II CATG 7 cut(s) 289, 342, 470, 609, 688, 832, 879
Hin6I GCGC 2 cut(s) 879, 926
HinP1I GCGC 2 cut(s) 879, 926
HpaII CCGG 2 cut(s) 12, 549
HphI GGTGA 2 cut(s) 101, 599
Hpy166II GTNNAC 3 cut(s) 18, 244, 803
Hpy188I TCNGA 3 cut(s) 253, 306, 424
Hpy188III TCNNGA 5 cut(s) 64, 476, 491, 549, 950
Hpy8I GTNNAC 3 cut(s) 18, 244, 803
HpyAV CCTTC 6 cut(s) 86, 473, 488, 689, 881, 895
HpyCH4III ACNGT 8 cut(s) 83, 89, 302, 319, 578, 584, 725, 817
HpyCH4IV ACGT 1 cut(s) 262
HpyCH4V TGCA 8 cut(s) 181, 338, 466, 593, 743, 911, 940, 1013
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 223, 335, 925
HpyF3I CTNAG 2 cut(s) 150, 753
HpySE526I ACGT 1 cut(s) 262
Hsp92II CATG 7 cut(s) 289, 342, 470, 609, 688, 832, 879
HspAI GCGC 2 cut(s) 879, 926
Kpn2I TCCGGA 1 cut(s) 548
Kzo9I GATC 4 cut(s) 4, 487, 551, 946
LmnI GCTCC 1 cut(s) 497
LweI GCATC 2 cut(s) 580, 1000
MabI ACCWGGT 1 cut(s) 646
MaeI CTAG 3 cut(s) 186, 353, 567
MaeII ACGT 1 cut(s) 262
MaeIII GTNAC 4 cut(s) 83, 578, 663, 977
MalI GATC 4 cut(s) 6, 489, 553, 948
MboI GATC 4 cut(s) 4, 487, 551, 946
MboII GAAGA 8 cut(s) 274, 319, 437, 617, 649, 701, 706, 855
MflI RGATCY 2 cut(s) 4, 551
MhlI GDGCHC 1 cut(s) 702
MluCI AATT 4 cut(s) 195, 254, 859, 941
MlyI GAGTC 1 cut(s) 985
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 8 cut(s) 133, 354, 633, 667, 712, 722, 787, 998
Mph1103I ATGCAT 1 cut(s) 340
MroI TCCGGA 1 cut(s) 548
MseI TTAA 5 cut(s) 146, 333, 512, 746, 1018
MspA1I CMGCKG 2 cut(s) 409, 459
MspI CCGG 2 cut(s) 12, 549
MspR9I CCNGG 6 cut(s) 13, 433, 630, 648, 669, 807
MvaI CCWGG 5 cut(s) 433, 630, 648, 669, 807
MvnI CGCG 2 cut(s) 881, 928
MwoI GCNNNNNNNGC 4 cut(s) 54, 223, 335, 925
NciI CCSGG 1 cut(s) 13
NcoI CCATGG 1 cut(s) 828
NdeI CATATG 2 cut(s) 534, 613
NdeII GATC 4 cut(s) 4, 487, 551, 946
NlaIII CATG 7 cut(s) 289, 342, 470, 609, 688, 832, 879
NlaIV GGNNCC 3 cut(s) 6, 16, 699
NmuCI GTSAC 2 cut(s) 83, 977
NsiI ATGCAT 1 cut(s) 340
PfeI GAWTC 9 cut(s) 24, 51, 113, 170, 233, 376, 596, 736, 824
PkrI GCNGC 3 cut(s) 411, 438, 458
PleI GAGTC 1 cut(s) 984
PpsI GAGTC 1 cut(s) 984
PpuMI RGGWCCY 1 cut(s) 676
Psp5II RGGWCCY 1 cut(s) 676
Psp6I CCWGG 5 cut(s) 431, 628, 646, 667, 805
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 5 cut(s) 431, 628, 646, 667, 805
PspN4I GGNNCC 3 cut(s) 6, 16, 699
PspPI GGNCC 3 cut(s) 15, 676, 930
PspPPI RGGWCCY 1 cut(s) 676
PsuI RGATCY 2 cut(s) 4, 551
RsaI GTAC 4 cut(s) 539, 610, 727, 802
RsaNI GTAC 4 cut(s) 538, 609, 726, 801
SaqAI TTAA 5 cut(s) 146, 333, 512, 746, 1018
SatI GCNGC 3 cut(s) 410, 437, 457
Sau3AI GATC 4 cut(s) 4, 487, 551, 946
Sau96I GGNCC 3 cut(s) 15, 676, 930
SchI GAGTC 1 cut(s) 985
ScrFI CCNGG 6 cut(s) 13, 433, 630, 648, 669, 807
SduI GDGCHC 1 cut(s) 702
SexAI ACCWGGT 1 cut(s) 646
SfaNI GCATC 2 cut(s) 580, 1000
SinI GGWCC 3 cut(s) 15, 676, 930
Sse9I AATT 4 cut(s) 195, 254, 859, 941
SsiI CCGC 8 cut(s) 226, 329, 409, 436, 457, 708, 881, 928
SspI AATATT 1 cut(s) 131
SspMI CTAG 3 cut(s) 186, 353, 567
StyD4I CCNGG 6 cut(s) 11, 431, 628, 646, 667, 805
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 8 cut(s) 83, 89, 302, 319, 578, 584, 725, 817
TaiI ACGT 1 cut(s) 265
TaqI TCGA 4 cut(s) 22, 27, 236, 988
TasI AATT 4 cut(s) 195, 254, 859, 941
TatI WGTACW 4 cut(s) 537, 608, 725, 800
TauI GCSGC 3 cut(s) 412, 439, 459
TfiI GAWTC 9 cut(s) 24, 51, 113, 170, 233, 376, 596, 736, 824
Tru1I TTAA 5 cut(s) 146, 333, 512, 746, 1018
Tru9I TTAA 5 cut(s) 146, 333, 512, 746, 1018
TscAI CASTG 2 cut(s) 322, 861
TseFI GTSAC 2 cut(s) 83, 977
Tsp45I GTSAC 2 cut(s) 83, 977
TspDTI ATGAA 4 cut(s) 219, 274, 483, 954
TspRI CASTG 2 cut(s) 322, 861
VpaK11BI GGWCC 3 cut(s) 15, 676, 930
XapI RAATTY 2 cut(s) 254, 859
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 3 cut(s) 186, 353, 567
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.