AT3G30380

Alpha beta hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011381
Physical Location & Seq
Forward (+)
11973971 .. 11976909
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G30380.2

Sequence Viewer

Length: 1200 bp
ATGGGAGCGGTGACGTCATCAATGGCGGCGAAGTTTGCGTTTTTCCCGCCAAATCCGCCGTCGTACGGCGTAGAAGTGGTGGAAGGAAAGCTGAGGCTGATTGGTGTAGAGAACGTGAAGGAGAATGTTGAAGTATTGAAGCTGAAGACGAAGAGAGGTAATCAGGTGGTTGCGGCGTATATTAAGAATCCTACAGCTTCACTTACGCTTCTTTATTCTCATGGTAACGCCGCGGATCTTGGTCAGATGTTCGAGCTTTTCTCCGAGCTTAGCCTCCATCTTCGTGTTAACTTAATTGGGTATGATTATTCGGGTTATGGGAGATCTTCAGGGAAGCCTAGTGAGCAGAACACGTATAGCGATATAGAAGCAGTGTATAGATGCTTGGAAGAGAAGTATGGTGTGAAGGAACAAGATGTTATCTTATATGGACAATCTGTAGGAAGCGGCCCGACTTTAGAATTGGCTTCTCGGTTACCTAACCTACGAGCCGTGGTTCTTCATAGCGCTATTGCTTCCGGACTTAGAGTCATGTACCCTGTTAAACGAACTTACTGGTTCGACATTTACAAGAATGTCGAGAAGATATCGTTTGTCAAATGTCCGGTTTTAGTAATACACGGAACATCAGATGATGTTGTTAATTGGTCTCATGGGAAGCAATTATTTGAGCTATGTAAAGAAAAATATGAACCGCTTTGGATCAAAGGAGGGAACCATTGTGATTTAGAGTTATATCCGCAATACATAAAGCATCTAAGAAAATTTGTATCGGCGATTGAAAAGTCGCCGATACTTAGAAATGGACCGGTGCCACTAACAGAGAAGGCAAGGAGTAGTACGGATATTAGAGAACCTGCAAGACCAAGCACAGATCAGAGAGATAAATCAAGAACAAGCACGGATCAAAGAGAGATGCCGAAGCTGAGTACAGATATAGCGAGAGCAAGCGTTGATAAGAGGGAGAGAACGAGAAGAAGCGTTGATGGGACTGAGAAACCGAGTAATTCCGCGGAACAGCAACTAGTACAACCAGAGAAAGGAAGGAATAGCATTGACAGGTACATTTCTCAGAGTTTCTATGATGTGTTGATGATCATGTTGTTGATGATCATGTATGATAACAGGTTTGGGGAGATGATAAGATCAGTAGGATTTTGCAACATAGATTGTTTCAAGCCTACAGCAACAGCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S9

Protein Analysis

399

Amino Acids

45.03

Weight (kDa)

9.05

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 172 2.1e-06 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 70 - 170 6.2e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30380 AT3G30380
fragaria_vesca FvH4_6g46130 FvH4_6g46130
malus_domestica MD09G1076700.v1.1 MD17G1067200.v1.1
prunus_persica Prupe.3G247400_v2.0.a1 Prupe.3G247400_v2.0.a1
pyrus_communis pycom09g00390 pycom17g06750
rosa_chinensis RchiOBHm_Chr2g0164581
rosa_laevigata RLG00000021467
rosa_multiflora Rmu_co8187214.1_g000001
rosa_roxburghii Rroxscaffold_2G00086470
rosa_rugosa Rorug02G0509300
rosa_samantha Rh2AG576900 Rh2BG588000 Rh2CG558800 Rh2DG598600
rosa_wichuraiana Rw2G047900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 17
Acc36I ACCTGC 1 cut(s) 865
AccB1I GGYRCC 1 cut(s) 811
AccBSI CCGCTC 1 cut(s) 8
AccII CGCG 2 cut(s) 233, 1013
AccIII TCCGGA 1 cut(s) 518
AclWI GGATC 3 cut(s) 243, 710, 912
AcsI RAATTY 1 cut(s) 764
AcuI CTGAAG 2 cut(s) 164, 312
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 6 cut(s) 65, 536, 841, 931, 1029, 1064
AfeI AGCGCT 1 cut(s) 508
AfiI CCNNNNNNNGG 2 cut(s) 65, 1040
AflIII ACRYGT 1 cut(s) 351
AgeI ACCGGT 1 cut(s) 808
AgsI TTSAA 4 cut(s) 131, 139, 782, 1177
AhdI GACNNNNNGTC 1 cut(s) 527
AhlI ACTAGT 1 cut(s) 1024
AluBI AGCT 8 cut(s) 91, 142, 197, 256, 268, 673, 925, 1193
AluI AGCT 8 cut(s) 91, 142, 197, 256, 268, 673, 925, 1193
Alw26I GTCTC 1 cut(s) 654
AlwI GGATC 3 cut(s) 243, 710, 912
Aor13HI TCCGGA 1 cut(s) 518
Aor51HI AGCGCT 1 cut(s) 508
AoxI GGCC 1 cut(s) 448
ApoI RAATTY 1 cut(s) 764
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AsiGI ACCGGT 1 cut(s) 808
AspLEI GCGC 1 cut(s) 509
AspS9I GGNCC 2 cut(s) 449, 806
AsuHPI GGTGA 1 cut(s) 22
AvaII GGWCC 1 cut(s) 806
BanI GGYRCC 1 cut(s) 811
BbsI GAAGAC 1 cut(s) 152
BbvCI CCTCAGC 1 cut(s) 92
BccI CCATC 2 cut(s) 285, 980
BceAI ACGGC 3 cut(s) 43, 82, 476
BclI TGATCA 2 cut(s) 1095, 1110
BcoDI GTCTC 1 cut(s) 654
BcuI ACTAGT 1 cut(s) 1024
BfaI CTAG 2 cut(s) 339, 1025
BfmI CTRYAG 3 cut(s) 192, 438, 1182
BfoI RGCGCY 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 865
BglII AGATCT 1 cut(s) 323
BisI GCNGC 4 cut(s) 27, 174, 231, 448
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 4 cut(s) 28, 175, 232, 449
Bme18I GGWCC 1 cut(s) 806
BmeRI GACNNNNNGTC 1 cut(s) 527
BmgT120I GGNCC 2 cut(s) 449, 806
BmiI GGNNCC 2 cut(s) 716, 813
BmsI GCATC 3 cut(s) 371, 763, 906
BpiI GAAGAC 1 cut(s) 152
BplI GAGNNNNNCTC 2 cut(s) 245, 277
Bpu10I CCTNAGC 1 cut(s) 92
Bpu1102I GCTNAGC 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 654
BsaJI CCNNGG 3 cut(s) 231, 492, 1011
BsaWI WCCGGW 3 cut(s) 518, 604, 808
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 1040
Bse118I RCCGGY 1 cut(s) 808
Bse1I ACTGG 1 cut(s) 560
BseAI TCCGGA 1 cut(s) 518
BseDI CCNNGG 3 cut(s) 231, 492, 1011
BseLI CCNNNNNNNGG 2 cut(s) 65, 1040
BseMII CTCAG 4 cut(s) 83, 917, 984, 1085
BseNI ACTGG 1 cut(s) 560
Bsh1236I CGCG 2 cut(s) 233, 1013
BshFI GGCC 1 cut(s) 450
BshNI GGYRCC 1 cut(s) 811
BshTI ACCGGT 1 cut(s) 808
BsiSI CCGG 3 cut(s) 519, 605, 809
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 1003
BslI CCNNNNNNNGG 2 cut(s) 65, 1040
BsmAI GTCTC 1 cut(s) 654
BsmFI GGGAC 1 cut(s) 1003
BsnI GGCC 1 cut(s) 450
Bso31I GGTCTC 1 cut(s) 654
Bsp13I TCCGGA 1 cut(s) 518
Bsp143I GATC 8 cut(s) 235, 323, 702, 874, 904, 1095, 1110, 1145
Bsp1720I GCTNAGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 4 cut(s) 84, 918, 985, 1084
BspEI TCCGGA 1 cut(s) 518
BspFNI CGCG 2 cut(s) 233, 1013
BspLI GGNNCC 2 cut(s) 716, 813
BspMI ACCTGC 1 cut(s) 865
BspPI GGATC 3 cut(s) 243, 710, 912
BspT107I GGYRCC 1 cut(s) 811
BspTNI GGTCTC 1 cut(s) 654
BsrBI CCGCTC 1 cut(s) 8
BsrFI RCCGGY 1 cut(s) 808
BsrI ACTGG 1 cut(s) 560
BssAI RCCGGY 1 cut(s) 808
BssECI CCNNGG 3 cut(s) 231, 492, 1011
BssMI GATC 8 cut(s) 235, 323, 702, 874, 904, 1095, 1110, 1145
BssNI GRCGYC 1 cut(s) 14
Bst6I CTCTTC 2 cut(s) 146, 384
BstACI GRCGYC 1 cut(s) 14
BstBAI YACGTR 1 cut(s) 354
BstC8I GCNNGC 1 cut(s) 949
BstDEI CTNAG 9 cut(s) 92, 269, 524, 758, 797, 926, 993, 1071, 1194
BstDSI CCRYGG 3 cut(s) 231, 492, 1011
BstEII GGTNACC 1 cut(s) 474
BstFNI CGCG 2 cut(s) 233, 1013
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 8 cut(s) 238, 326, 705, 877, 907, 1098, 1113, 1148
BstMAI GTCTC 1 cut(s) 654
BstMBI GATC 8 cut(s) 235, 323, 702, 874, 904, 1095, 1110, 1145
BstMWI GCNNNNNNNGC 3 cut(s) 35, 55, 343
BstPI GGTNACC 1 cut(s) 474
BstSFI CTRYAG 3 cut(s) 192, 438, 1182
BstUI CGCG 2 cut(s) 233, 1013
BstV2I GAAGAC 1 cut(s) 152
BstX2I RGATCY 2 cut(s) 235, 323
BstYI RGATCY 2 cut(s) 235, 323
BsuRI GGCC 1 cut(s) 450
BtgI CCRYGG 3 cut(s) 231, 492, 1011
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 378
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 1 cut(s) 949
CfoI GCGC 1 cut(s) 509
Cfr10I RCCGGY 1 cut(s) 808
Cfr13I GGNCC 2 cut(s) 449, 806
Cfr42I CCGCGG 2 cut(s) 234, 1014
Csp6I GTAC 6 cut(s) 64, 535, 840, 930, 1028, 1063
CspAI ACCGGT 1 cut(s) 808
CviAII CATG 5 cut(s) 221, 532, 653, 1099, 1114
CviQI GTAC 6 cut(s) 64, 535, 840, 930, 1028, 1063
DdeI CTNAG 9 cut(s) 92, 269, 524, 758, 797, 926, 993, 1071, 1194
DpnI GATC 8 cut(s) 237, 325, 704, 876, 906, 1097, 1112, 1147
DpnII GATC 8 cut(s) 235, 323, 702, 874, 904, 1095, 1110, 1145
DriI GACNNNNNGTC 1 cut(s) 527
Eam1104I CTCTTC 2 cut(s) 146, 384
Eam1105I GACNNNNNGTC 1 cut(s) 527
EarI CTCTTC 2 cut(s) 146, 384
EciI GGCGGA 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 654
Eco32I GATATC 1 cut(s) 588
Eco47I GGWCC 1 cut(s) 806
Eco47III AGCGCT 1 cut(s) 508
Eco57I CTGAAG 2 cut(s) 164, 312
Eco91I GGTNACC 1 cut(s) 474
EcoO65I GGTNACC 1 cut(s) 474
EcoRV GATATC 1 cut(s) 588
FaeI CATG 5 cut(s) 224, 535, 656, 1102, 1117
FaqI GGGAC 1 cut(s) 1003
FatI CATG 5 cut(s) 220, 531, 652, 1098, 1113
FauI CCCGC 1 cut(s) 54
FbaI TGATCA 2 cut(s) 1095, 1110
Fnu4HI GCNGC 4 cut(s) 27, 174, 231, 448
Fsp4HI GCNGC 4 cut(s) 27, 174, 231, 448
FspBI CTAG 2 cut(s) 339, 1025
GlaI GCGC 1 cut(s) 508
GluI GCNGC 4 cut(s) 27, 174, 231, 448
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 1 cut(s) 450
HapII CCGG 3 cut(s) 519, 605, 809
HhaI GCGC 1 cut(s) 509
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 5 cut(s) 224, 535, 656, 1102, 1117
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 2 cut(s) 187, 528
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 3 cut(s) 519, 605, 809
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 5 cut(s) 246, 265, 631, 879, 1074
Hpy188III TCNNGA 3 cut(s) 519, 580, 891
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 5 cut(s) 77, 112, 400, 820, 1038
HpyCH4IV ACGT 3 cut(s) 14, 114, 353
HpyCH4V TGCA 2 cut(s) 860, 1161
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 55, 343
HpyF3I CTNAG 9 cut(s) 92, 269, 524, 758, 797, 926, 993, 1071, 1194
HpySE526I ACGT 3 cut(s) 14, 114, 353
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 5 cut(s) 224, 535, 656, 1102, 1117
HspAI GCGC 1 cut(s) 507
Kpn2I TCCGGA 1 cut(s) 518
Ksp22I TGATCA 2 cut(s) 1095, 1110
KspAI GTTAAC 1 cut(s) 289
KspI CCGCGG 2 cut(s) 234, 1014
Kzo9I GATC 8 cut(s) 235, 323, 702, 874, 904, 1095, 1110, 1145
LmnI GCTCC 1 cut(s) 5
LweI GCATC 3 cut(s) 371, 763, 906
MaeI CTAG 2 cut(s) 339, 1025
MaeII ACGT 3 cut(s) 14, 114, 353
MaeIII GTNAC 3 cut(s) 10, 224, 474
MalI GATC 8 cut(s) 237, 325, 704, 876, 906, 1097, 1112, 1147
MbiI CCGCTC 1 cut(s) 8
MboI GATC 8 cut(s) 235, 323, 702, 874, 904, 1095, 1110, 1145
MboII GAAGA 8 cut(s) 157, 163, 272, 318, 401, 491, 595, 987
MflI RGATCY 2 cut(s) 235, 323
MluCI AATT 6 cut(s) 294, 461, 643, 662, 764, 1006
MlyI GAGTC 1 cut(s) 537
MnlI CCTC 5 cut(s) 87, 149, 284, 704, 954
MroI TCCGGA 1 cut(s) 518
MseI TTAA 5 cut(s) 183, 288, 293, 543, 642
MslI CAYNNNNRTG 1 cut(s) 282
MspA1I CMGCKG 2 cut(s) 233, 1013
MspI CCGG 3 cut(s) 519, 605, 809
MvnI CGCG 2 cut(s) 233, 1013
MwoI GCNNNNNNNGC 3 cut(s) 35, 55, 343
NdeII GATC 8 cut(s) 235, 323, 702, 874, 904, 1095, 1110, 1145
NlaIII CATG 5 cut(s) 224, 535, 656, 1102, 1117
NlaIV GGNNCC 2 cut(s) 716, 813
NmuCI GTSAC 1 cut(s) 10
PfeI GAWTC 1 cut(s) 187
Pfl23II CGTACG 1 cut(s) 63
PinAI ACCGGT 1 cut(s) 808
PkrI GCNGC 4 cut(s) 28, 175, 232, 449
PleI GAGTC 1 cut(s) 536
PpsI GAGTC 1 cut(s) 536
Ppu21I YACGTR 1 cut(s) 354
PspEI GGTNACC 1 cut(s) 474
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 716, 813
PspPI GGNCC 2 cut(s) 449, 806
PsuI RGATCY 2 cut(s) 235, 323
RsaI GTAC 6 cut(s) 65, 536, 841, 931, 1029, 1064
RsaNI GTAC 6 cut(s) 64, 535, 840, 930, 1028, 1063
RseI CAYNNNNRTG 1 cut(s) 282
SacII CCGCGG 2 cut(s) 234, 1014
SaqAI TTAA 5 cut(s) 183, 288, 293, 543, 642
SatI GCNGC 4 cut(s) 27, 174, 231, 448
Sau3AI GATC 8 cut(s) 235, 323, 702, 874, 904, 1095, 1110, 1145
Sau96I GGNCC 2 cut(s) 449, 806
SchI GAGTC 1 cut(s) 537
SfaNI GCATC 3 cut(s) 371, 763, 906
SfcI CTRYAG 3 cut(s) 192, 438, 1182
Sfr303I CCGCGG 2 cut(s) 234, 1014
SgrBI CCGCGG 2 cut(s) 234, 1014
SinI GGWCC 1 cut(s) 806
SmiMI CAYNNNNRTG 1 cut(s) 282
SpeI ACTAGT 1 cut(s) 1024
Sse9I AATT 6 cut(s) 294, 461, 643, 662, 764, 1006
SspMI CTAG 2 cut(s) 339, 1025
TaiI ACGT 3 cut(s) 17, 117, 356
TaqI TCGA 3 cut(s) 252, 561, 579
TasI AATT 6 cut(s) 294, 461, 643, 662, 764, 1006
TatI WGTACW 2 cut(s) 929, 1027
TauI GCSGC 4 cut(s) 29, 176, 233, 450
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 5 cut(s) 183, 288, 293, 543, 642
Tru9I TTAA 5 cut(s) 183, 288, 293, 543, 642
TscAI CASTG 1 cut(s) 378
TseFI GTSAC 1 cut(s) 10
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 491, 705
TspGWI ACGGA 3 cut(s) 636, 857, 917
TspRI CASTG 1 cut(s) 378
VpaK11BI GGWCC 1 cut(s) 806
XapI RAATTY 1 cut(s) 764
XspI CTAG 2 cut(s) 339, 1025
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.