Rh2DG598600

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011381
Physical Location & Seq
Reverse (-)
83091263 .. 83095461
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG598600.1

Sequence Viewer

Length: 1143 bp
ATGGGGGCGGTGACGTCATCCATGGCGGCGAAGTTCGCGTTCTTCCCGCCGCACCCGCCGTCGTACGGGGTGGAGGAGGAGGAGGAGAGCGGGAAGCTGAGGATGGCGGGAGTGCCGGCGAGGGCGAACGTCGACGTTTTGAAGCTGAAGACGAAGAGGGGAAACGACGTCGTCGCGGTTTACTTTAAGAACCCGGCGGCGAAGCTCACCGTTCTGCACTCCCACGGTAACGCAGCTGATCTGGGCCAGATGTATGAGCTCTTCAGCGAGCTCAGTCTTCACCTCCGAGTCAACTTAATAGGGTATGATTATTCTGGGTACGGACAATCTACTGGGAAGCCAAGTGAGCAAAACACTTATGCAGACATAGAAGCTGTATATAGATGTCTCGTGGAGAAGTACGGTGCAAAGGAGGAGGATGTAATTTTATACGGGCAATCAGTTGGCAGTGGGCCTACTCTAGATCTGGCAACTCGCTTACCGAAATTGAGGGCAGTGGTTCTTCACAGTCCGATCATGTCTGGCCTTCGAGTCATGTATCCGGTGAAGCGATCGTACTGGTTTGACATTTACAAGAACATTGACAAAATTCCGTTGGTCAATTGCCCTGTTTTAGTTATCCATGGGACAGCTGATGATGTTGTGGATTGGTCACATGGCAAGCAACTGTGGGAACACTGTAAGGAGAAGTACGAGCCATTGTGGATAAAAGGAGGGAACCATTGTGACTTGGAGCTCTACCCACAGTACATCAAGCATCTGAAGAAGTTTGTATCGGCCATTGAGAAGTTTCCACGTCTTAAAAATGGATCTGCACCAGTTACTGATCAACTAGAAAAGCCCCGAGGCAGCACCGACATCAGGGAGAAACCTTGGTCCAACACAGATCATAAAGAGGTTTCCAGAACAAGTACTGACCATAGAGAAAACCCTAGGGTAAGCACAGACTGCAGAGAGAAGTCAAGGGCCAGCACTGATCAGAGAGATAGATCAAAAAAGATTATGGATCAACCTGAAAAGCCAAGTAATAGCGTGGAGCAGCCGGAGAAAGCAAGGAATAGCATTGACCGCTTTGGGGAGATGTTCAGATCAGTTGGCTTCTGCAATATTGATTGTTTTAGGCCCACAGCGACCAGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S9

Protein Analysis

380

Amino Acids

42.9

Weight (kDa)

8.63

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 67 - 174 1.5e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 72 - 173 9.2e-08 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30380 AT3G30380
fragaria_vesca FvH4_6g46130 FvH4_6g46130
malus_domestica MD09G1076700.v1.1 MD17G1067200.v1.1
prunus_persica Prupe.3G247400_v2.0.a1 Prupe.3G247400_v2.0.a1
pyrus_communis pycom09g00390 pycom17g06750
rosa_chinensis RchiOBHm_Chr2g0164581
rosa_laevigata RLG00000021467
rosa_multiflora Rmu_co8187214.1_g000001
rosa_roxburghii Rroxscaffold_2G00086470
rosa_rugosa Rorug02G0509300
rosa_samantha Rh2AG576900 Rh2BG588000 Rh2CG558800 Rh2DG598600
rosa_wichuraiana Rw2G047900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 17, 171
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 132
AccII CGCG 2 cut(s) 38, 176
AclWI GGATC 2 cut(s) 817, 1014
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 1 cut(s) 588
AcuI CTGAAG 3 cut(s) 167, 247, 782
AcyI GRCGYC 2 cut(s) 14, 168
AfaI GTAC 7 cut(s) 65, 320, 401, 557, 692, 749, 913
AfiI CCNNNNNNNGG 3 cut(s) 65, 861, 1075
AgsI TTSAA 1 cut(s) 142
AjiI CACGTC 1 cut(s) 797
AluBI AGCT 9 cut(s) 97, 145, 205, 236, 259, 271, 374, 632, 736
AluI AGCT 9 cut(s) 97, 145, 205, 236, 259, 271, 374, 632, 736
Alw21I GWGCWC 3 cut(s) 261, 273, 738
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 2 cut(s) 817, 1014
AlwNI CAGNNNCTG 1 cut(s) 824
Ama87I CYCGRG 1 cut(s) 843
AoxI GGCC 6 cut(s) 244, 452, 523, 777, 966, 1121
ApeKI GCWGC 3 cut(s) 233, 849, 1039
ApoI RAATTY 1 cut(s) 588
AspA2I CCTAGG 1 cut(s) 932
AspS9I GGNCC 5 cut(s) 244, 452, 876, 966, 1122
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 4 cut(s) 22, 199, 272, 556
AvaI CYCGRG 1 cut(s) 843
AvaII GGWCC 1 cut(s) 876
AvrII CCTAGG 1 cut(s) 932
BanII GRGCYC 3 cut(s) 261, 273, 738
BarI GAAGNNNNNNTAC 4 cut(s) 245, 277, 539, 571
BauI CACGAG 1 cut(s) 389
BbsI GAAGAC 2 cut(s) 155, 269
Bbv12I GWGCWC 3 cut(s) 261, 273, 738
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 245, 861, 1051
BccI CCATC 1 cut(s) 97
BceAI ACGGC 1 cut(s) 43
BciVI GTATCC 1 cut(s) 549
BclI TGATCA 2 cut(s) 826, 976
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 3 cut(s) 461, 833, 933
BfmI CTRYAG 1 cut(s) 949
BfuI GTATCC 1 cut(s) 549
BglII AGATCT 1 cut(s) 463
BisI GCNGC 6 cut(s) 27, 50, 198, 234, 850, 1040
BlnI CCTAGG 1 cut(s) 932
BlsI GCNGC 6 cut(s) 28, 51, 199, 235, 851, 1041
BmcAI AGTACT 1 cut(s) 913
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 1 cut(s) 876
BmeT110I CYCGRG 1 cut(s) 843
BmgBI CACGTC 1 cut(s) 797
BmgT120I GGNCC 5 cut(s) 244, 452, 876, 966, 1122
BmiI GGNNCC 1 cut(s) 719
BmrFI CCNGG 1 cut(s) 194
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 766
BmuI ACTGGG 1 cut(s) 342
BpiI GAAGAC 2 cut(s) 155, 269
Bpu10I CCTNAGC 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 2 cut(s) 14, 168
BsaJI CCNNGG 6 cut(s) 21, 223, 622, 844, 872, 932
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 861, 1075
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 4 cut(s) 337, 563, 818, 1134
BseDI CCNNGG 6 cut(s) 21, 223, 622, 844, 872, 932
BseGI GGATG 3 cut(s) 17, 108, 424
BseLI CCNNNNNNNGG 3 cut(s) 65, 861, 1075
BseMII CTCAG 2 cut(s) 89, 286
BseNI ACTGG 4 cut(s) 337, 563, 818, 1134
BseRI GAGGAG 5 cut(s) 89, 92, 95, 98, 428
BseXI GCAGC 3 cut(s) 245, 861, 1051
BsgI GTGCAG 2 cut(s) 200, 798
Bsh1236I CGCG 2 cut(s) 38, 176
Bsh1285I CGRYCG 1 cut(s) 554
BshFI GGCC 6 cut(s) 246, 454, 525, 779, 968, 1123
BsiEI CGRYCG 1 cut(s) 554
BsiHKAI GWGCWC 3 cut(s) 261, 273, 738
BsiHKCI CYCGRG 1 cut(s) 843
BsiSI CCGG 4 cut(s) 116, 194, 542, 1043
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 640
BslI CCNNNNNNNGG 3 cut(s) 65, 861, 1075
BsmAI GTCTC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 640
BsnI GGCC 6 cut(s) 246, 454, 525, 779, 968, 1123
BsoBI CYCGRG 1 cut(s) 843
Bsp1286I GDGCHC 3 cut(s) 261, 273, 738
Bsp19I CCATGG 2 cut(s) 21, 622
BspANI GGCC 6 cut(s) 246, 454, 525, 779, 968, 1123
BspCNI CTCAG 2 cut(s) 90, 285
BspFNI CGCG 2 cut(s) 38, 176
BspLI GGNNCC 1 cut(s) 719
BspMAI CTGCAG 1 cut(s) 953
BspPI GGATC 2 cut(s) 817, 1014
BspQI GCTCTTC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 4 cut(s) 337, 563, 818, 1134
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 6 cut(s) 21, 223, 622, 844, 872, 932
BssNI GRCGYC 2 cut(s) 14, 168
BssSI CACGAG 1 cut(s) 389
BssT1I CCWWGG 4 cut(s) 21, 622, 872, 932
Bst2BI CACGAG 1 cut(s) 389
Bst4CI ACNGT 7 cut(s) 211, 227, 404, 509, 669, 680, 747
Bst6I CTCTTC 2 cut(s) 149, 266
BstACI GRCGYC 2 cut(s) 14, 168
BstAPI GCANNNNNTGC 1 cut(s) 948
BstC8I GCNNGC 4 cut(s) 117, 269, 662, 970
BstDEI CTNAG 2 cut(s) 98, 272
BstDSI CCRYGG 3 cut(s) 21, 223, 622
BstF5I GGATG 3 cut(s) 17, 108, 424
BstFNI CGCG 2 cut(s) 38, 176
BstMAI GTCTC 1 cut(s) 392
BstMCI CGRYCG 1 cut(s) 554
BstMWI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 948, 1068
BstSCI CCNGG 1 cut(s) 192
BstSFI CTRYAG 1 cut(s) 949
BstUI CGCG 2 cut(s) 38, 176
BstV1I GCAGC 3 cut(s) 245, 861, 1051
BstV2I GAAGAC 2 cut(s) 155, 269
BstX2I RGATCY 2 cut(s) 463, 809
BstYI RGATCY 2 cut(s) 463, 809
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 6 cut(s) 246, 454, 525, 779, 968, 1123
BtgI CCRYGG 3 cut(s) 21, 223, 622
BtrI CACGTC 1 cut(s) 797
BtsCI GGATG 3 cut(s) 17, 108, 424
BtsI GCAGTG 2 cut(s) 454, 501
BtsIMutI CAGTG 5 cut(s) 454, 501, 676, 972, 1141
Cac8I GCNNGC 4 cut(s) 117, 269, 662, 970
CaiI CAGNNNCTG 1 cut(s) 824
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 5 cut(s) 244, 452, 876, 966, 1122
Csp6I GTAC 7 cut(s) 64, 319, 400, 556, 691, 748, 912
CviAII CATG 5 cut(s) 22, 517, 535, 623, 656
CviQI GTAC 7 cut(s) 64, 319, 400, 556, 691, 748, 912
DdeI CTNAG 2 cut(s) 98, 272
EaeI YGGCCR 1 cut(s) 777
Eam1104I CTCTTC 2 cut(s) 149, 266
EarI CTCTTC 2 cut(s) 149, 266
Ecl136II GAGCTC 3 cut(s) 259, 271, 736
Eco130I CCWWGG 4 cut(s) 21, 622, 872, 932
Eco24I GRGCYC 3 cut(s) 261, 273, 738
Eco47I GGWCC 1 cut(s) 876
Eco53kI GAGCTC 3 cut(s) 259, 271, 736
Eco57I CTGAAG 3 cut(s) 167, 247, 782
Eco88I CYCGRG 1 cut(s) 843
EcoICRI GAGCTC 3 cut(s) 259, 271, 736
EcoT14I CCWWGG 4 cut(s) 21, 622, 872, 932
EcoT38I GRGCYC 3 cut(s) 261, 273, 738
ErhI CCWWGG 4 cut(s) 21, 622, 872, 932
FaeI CATG 5 cut(s) 25, 520, 538, 626, 659
FaqI GGGAC 1 cut(s) 640
FatI CATG 5 cut(s) 21, 516, 534, 622, 655
FauI CCCGC 4 cut(s) 54, 63, 83, 100
FbaI TGATCA 2 cut(s) 826, 976
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 6 cut(s) 27, 50, 198, 234, 850, 1040
FokI GGATG 3 cut(s) 4, 115, 431
FriOI GRGCYC 3 cut(s) 261, 273, 738
Fsp4HI GCNGC 6 cut(s) 27, 50, 198, 234, 850, 1040
FspBI CTAG 3 cut(s) 461, 833, 933
GluI GCNGC 6 cut(s) 27, 50, 198, 234, 850, 1040
HaeIII GGCC 6 cut(s) 246, 454, 525, 779, 968, 1123
HapII CCGG 4 cut(s) 116, 194, 542, 1043
Hin1I GRCGYC 2 cut(s) 14, 168
Hin1II CATG 5 cut(s) 25, 520, 538, 626, 659
HincII GTYRAC 2 cut(s) 133, 292
HindII GTYRAC 2 cut(s) 133, 292
HinfI GANTC 2 cut(s) 288, 531
HpaII CCGG 4 cut(s) 116, 194, 542, 1043
HphI GGTGA 4 cut(s) 22, 199, 272, 556
Hpy166II GTNNAC 3 cut(s) 133, 181, 292
Hpy188I TCNGA 5 cut(s) 287, 513, 762, 981, 1088
Hpy188III TCNNGA 2 cut(s) 461, 903
Hpy8I GTNNAC 3 cut(s) 133, 181, 292
Hpy99I CGWCG 6 cut(s) 64, 134, 137, 170, 173, 176
HpyAV CCTTC 1 cut(s) 536
HpyCH4III ACNGT 7 cut(s) 211, 227, 404, 509, 669, 680, 747
HpyCH4IV ACGT 5 cut(s) 14, 129, 135, 168, 796
HpyCH4V TGCA 6 cut(s) 217, 362, 407, 815, 951, 1104
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 948, 1068
HpyF3I CTNAG 2 cut(s) 98, 272
HpySE526I ACGT 5 cut(s) 14, 129, 135, 168, 796
Hsp92I GRCGYC 2 cut(s) 14, 168
Hsp92II CATG 5 cut(s) 25, 520, 538, 626, 659
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 2 cut(s) 826, 976
LguI GCTCTTC 1 cut(s) 266
LmnI GCTCC 2 cut(s) 733, 1036
Lsp1109I GCAGC 3 cut(s) 245, 861, 1051
LweI GCATC 1 cut(s) 766
MaeI CTAG 3 cut(s) 461, 833, 933
MaeII ACGT 5 cut(s) 14, 129, 135, 168, 796
MaeIII GTNAC 5 cut(s) 10, 227, 651, 725, 820
MbiI CCGCTC 1 cut(s) 90
MboII GAAGA 7 cut(s) 34, 160, 166, 253, 269, 494, 775
MfeI CAATTG 1 cut(s) 601
MflI RGATCY 2 cut(s) 463, 809
MhlI GDGCHC 3 cut(s) 261, 273, 738
MluCI AATT 4 cut(s) 423, 485, 588, 601
MlyI GAGTC 2 cut(s) 297, 540
MmeI TCCRAC 1 cut(s) 903
MroNI GCCGGC 1 cut(s) 115
MseI TTAA 3 cut(s) 186, 296, 801
MspA1I CMGCKG 2 cut(s) 236, 632
MspI CCGG 4 cut(s) 116, 194, 542, 1043
MspR9I CCNGG 1 cut(s) 194
MunI CAATTG 1 cut(s) 601
MvnI CGCG 2 cut(s) 38, 176
MwoI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 948, 1068
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 2 cut(s) 21, 622
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 5 cut(s) 25, 520, 538, 626, 659
NlaIV GGNNCC 1 cut(s) 719
NmuCI GTSAC 3 cut(s) 10, 651, 725
PciSI GCTCTTC 1 cut(s) 266
PdiI GCCGGC 1 cut(s) 117
Pfl23II CGTACG 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 6 cut(s) 28, 51, 199, 235, 851, 1041
Ple19I CGATCG 1 cut(s) 554
PleI GAGTC 2 cut(s) 296, 539
PpsI GAGTC 2 cut(s) 296, 539
Psp124BI GAGCTC 3 cut(s) 261, 273, 738
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 719
PspPI GGNCC 5 cut(s) 244, 452, 876, 966, 1122
PstI CTGCAG 1 cut(s) 953
PstNI CAGNNNCTG 1 cut(s) 824
PsuI RGATCY 2 cut(s) 463, 809
PsyI GACNNNGTC 1 cut(s) 170
PvuI CGATCG 1 cut(s) 554
PvuII CAGCTG 2 cut(s) 236, 632
RsaI GTAC 7 cut(s) 65, 320, 401, 557, 692, 749, 913
RsaNI GTAC 7 cut(s) 64, 319, 400, 556, 691, 748, 912
SacI GAGCTC 3 cut(s) 261, 273, 738
SalI GTCGAC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 3 cut(s) 186, 296, 801
SatI GCNGC 6 cut(s) 27, 50, 198, 234, 850, 1040
Sau96I GGNCC 5 cut(s) 244, 452, 876, 966, 1122
ScaI AGTACT 1 cut(s) 913
SchI GAGTC 2 cut(s) 297, 540
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 3 cut(s) 261, 273, 738
SfaNI GCATC 1 cut(s) 766
SfcI CTRYAG 1 cut(s) 949
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 1 cut(s) 876
Sse9I AATT 4 cut(s) 423, 485, 588, 601
SspI AATATT 1 cut(s) 1108
SspMI CTAG 3 cut(s) 461, 833, 933
SstI GAGCTC 3 cut(s) 261, 273, 738
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 4 cut(s) 21, 622, 872, 932
TaaI ACNGT 7 cut(s) 211, 227, 404, 509, 669, 680, 747
TaiI ACGT 5 cut(s) 17, 132, 138, 171, 799
TaqI TCGA 2 cut(s) 132, 529
TasI AATT 4 cut(s) 423, 485, 588, 601
TatI WGTACW 2 cut(s) 747, 911
TauI GCSGC 3 cut(s) 29, 52, 200
Tru1I TTAA 3 cut(s) 186, 296, 801
Tru9I TTAA 3 cut(s) 186, 296, 801
TscAI CASTG 5 cut(s) 454, 501, 683, 979, 1141
TseFI GTSAC 3 cut(s) 10, 651, 725
TseI GCWGC 3 cut(s) 233, 849, 1039
Tsp45I GTSAC 3 cut(s) 10, 651, 725
TspGWI ACGGA 2 cut(s) 336, 582
TspRI CASTG 5 cut(s) 454, 501, 683, 979, 1141
Tth111I GACNNNGTC 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 876
XapI RAATTY 1 cut(s) 588
XbaI TCTAGA 1 cut(s) 460
XmaJI CCTAGG 1 cut(s) 932
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 3 cut(s) 461, 833, 933
ZraI GACGTC 2 cut(s) 15, 169
ZrmI AGTACT 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.