FvH4_6g46130

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011381
Physical Location & Seq
Reverse (-)
35244845 .. 35248067
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g46130.t2

Sequence Viewer

Length: 1143 bp
ATGGGGGCGGTGACGTCATCGATGGCGGCGAAGTTCGCGTTCTTCCCGCCGCAGCCGCCGTCGTACGGGGTGGAGGAGGAGGAGGAGAGCGGGAAGCTGAGGATGACGGGGGTGCCGGCGAGGTCGAACGTCGACGTTTTGAAGCTGAGGACGAAGAGGGGAAACGACGTCGTCGCCGTTTACTTTAAGAACCCGGCGGCGAAGCTCACCGTGCTGCACTCCCACGGTAACGCGGCTGATCTGGGCCAGATGTATGAGCTCTTCAGTGAGCTCAGTCTTCACCTCAGAGTCAACTTGTTGGGGTATGATTATTCTGGGTATGGACAATCTACTGGGAAGCCGAGTGAGCAGAACACTTATGCAGACATAGAAGCTGCGTATAGATGTCTCGTGGAGAAGTACGGTGCAAAGGAGGAGGATGTAATTTTATACGGGCAATCAGTTGGCAGTGGGCCTACTCTAGATCTGGCAACTCGCTTACCGAAATTAAGGGCAGTGGTTCTTCACAGCCCGATCATGTCTGGCCTTCGCGTCATGTATCCGGTGAAGCGATCGTACTGGTTTGACATTTACAAGAATATTGACAAAATACCATTGGTCAATTGTCCTGTTTTGGTTATCCATGGGACAGCTGATGATGTGGTGGATTGGTCACATGGCAAACAACTTTGGGAACACTGTAAGGAGAAGTATGAGCCATTGTGGATAAAAGGAGGGAACCATTGTGACCTAGAGCTCTACCCACAGTACATCAAGCATTTGAAGAAGTTTGTATCAGCTGTTGAGAAGTATCCACGCCTTAAAAAGGGATCTGCACCAGTTTCGGATCAACCAGAAAAGCCCCGGGGAAGCACCGACATCAGGGAGAAATCTTTGGCCAAGACAGATCATAAAGATGTTTCCAGAACAAGTACTGACCATAGAGAAAACCCTAGGGTAAGCACAGACTGCAGAGAGAAGTCAAGGGCCAGCACTGATAAGAGAGATAGATCAAAAAAGATTATGGATCAACCTGAAAAGCCAAGTAATAGCGTGGATCAGCCAGATAAAGCAAGGAATAGCATTGACCGCTTCGGGGAGATGTTCAGATCAGTTGGCTTCTGCAATATTGATTGTTTTAGGCCCACGGCAACCAGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S9

Protein Analysis

381

Amino Acids

42.79

Weight (kDa)

8.92

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 67 - 175 6.3e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 72 - 173 4.4e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30380 AT3G30380
fragaria_vesca FvH4_6g46130 FvH4_6g46130
malus_domestica MD09G1076700.v1.1 MD17G1067200.v1.1
prunus_persica Prupe.3G247400_v2.0.a1 Prupe.3G247400_v2.0.a1
pyrus_communis pycom09g00390 pycom17g06750
rosa_chinensis RchiOBHm_Chr2g0164581
rosa_laevigata RLG00000021467
rosa_multiflora Rmu_co8187214.1_g000001
rosa_roxburghii Rroxscaffold_2G00086470
rosa_rugosa Rorug02G0509300
rosa_samantha Rh2AG576900 Rh2BG588000 Rh2CG558800 Rh2DG598600
rosa_wichuraiana Rw2G047900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 17, 171
AccB1I GGYRCC 1 cut(s) 112
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 132
AccII CGCG 3 cut(s) 38, 233, 531
AciI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 197, 233, 1069
AclWI GGATC 4 cut(s) 817, 834, 1014, 1044
AcoI YGGCCR 1 cut(s) 876
AcuI CTGAAG 1 cut(s) 247
AcyI GRCGYC 2 cut(s) 14, 168
AfaI GTAC 5 cut(s) 65, 401, 557, 749, 913
AfiI CCNNNNNNNGG 4 cut(s) 65, 805, 861, 1075
AgsI TTSAA 2 cut(s) 142, 763
AluBI AGCT 9 cut(s) 97, 145, 205, 259, 271, 374, 632, 736, 779
AluI AGCT 9 cut(s) 97, 145, 205, 259, 271, 374, 632, 736, 779
Alw21I GWGCWC 3 cut(s) 261, 273, 738
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 4 cut(s) 817, 834, 1014, 1044
Ama87I CYCGRG 1 cut(s) 843
AoxI GGCC 6 cut(s) 244, 452, 523, 876, 966, 1121
ApeKI GCWGC 3 cut(s) 52, 214, 374
AspA2I CCTAGG 1 cut(s) 932
AspS9I GGNCC 4 cut(s) 244, 452, 966, 1122
AsuC2I CCSGG 3 cut(s) 194, 844, 845
AsuHPI GGTGA 4 cut(s) 22, 199, 272, 556
AvaI CYCGRG 1 cut(s) 843
AvrII CCTAGG 1 cut(s) 932
BalI TGGCCA 1 cut(s) 878
BanI GGYRCC 1 cut(s) 112
BanII GRGCYC 3 cut(s) 261, 273, 738
BarI GAAGNNNNNNTAC 4 cut(s) 245, 277, 539, 571
BauI CACGAG 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 269
Bbv12I GWGCWC 3 cut(s) 261, 273, 738
BbvCI CCTCAGC 2 cut(s) 98, 146
BbvI GCAGC 3 cut(s) 64, 201, 361
BccI CCATC 1 cut(s) 16
BceAI ACGGC 2 cut(s) 43, 161
BcgI CGANNNNNNTGC 2 cut(s) 804, 838
BciVI GTATCC 2 cut(s) 549, 801
BcnI CCSGG 3 cut(s) 194, 844, 845
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 3 cut(s) 461, 731, 933
BfmI CTRYAG 1 cut(s) 949
BfuI GTATCC 2 cut(s) 549, 801
BglII AGATCT 1 cut(s) 463
BisI GCNGC 8 cut(s) 27, 50, 53, 56, 198, 215, 234, 375
BlnI CCTAGG 1 cut(s) 932
BlsI GCNGC 8 cut(s) 28, 51, 54, 57, 199, 216, 235, 376
BmcAI AGTACT 1 cut(s) 913
Bme1390I CCNGG 3 cut(s) 194, 844, 845
BmeT110I CYCGRG 1 cut(s) 843
BmgT120I GGNCC 4 cut(s) 244, 452, 966, 1122
BmiI GGNNCC 2 cut(s) 114, 719
BmrFI CCNGG 3 cut(s) 194, 844, 845
BmrI ACTGGG 1 cut(s) 342
BmuI ACTGGG 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 269
Bpu10I CCTNAGC 2 cut(s) 98, 146
BpuMI CCSGG 3 cut(s) 194, 844, 845
Bsa29I ATCGAT 1 cut(s) 20
BsaHI GRCGYC 2 cut(s) 14, 168
BsaJI CCNNGG 7 cut(s) 223, 622, 842, 843, 844, 932, 1125
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 805, 861, 1075
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 4 cut(s) 337, 563, 818, 1134
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 7 cut(s) 223, 622, 842, 843, 844, 932, 1125
BseGI GGATG 2 cut(s) 108, 424
BseLI CCNNNNNNNGG 4 cut(s) 65, 805, 861, 1075
BseMII CTCAG 4 cut(s) 89, 137, 286, 298
BseNI ACTGG 4 cut(s) 337, 563, 818, 1134
BseRI GAGGAG 5 cut(s) 89, 92, 95, 98, 428
BseXI GCAGC 3 cut(s) 64, 201, 361
BsgI GTGCAG 2 cut(s) 200, 798
Bsh1236I CGCG 3 cut(s) 38, 233, 531
Bsh1285I CGRYCG 1 cut(s) 554
BshFI GGCC 6 cut(s) 246, 454, 525, 878, 968, 1123
BshNI GGYRCC 1 cut(s) 112
BshVI ATCGAT 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 554
BsiHKAI GWGCWC 3 cut(s) 261, 273, 738
BsiHKCI CYCGRG 1 cut(s) 843
BsiSI CCGG 4 cut(s) 116, 194, 542, 844
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 640
BslI CCNNNNNNNGG 4 cut(s) 65, 805, 861, 1075
BsmAI GTCTC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 640
BsnI GGCC 6 cut(s) 246, 454, 525, 878, 968, 1123
BsoBI CYCGRG 1 cut(s) 843
Bsp1286I GDGCHC 3 cut(s) 261, 273, 738
Bsp19I CCATGG 1 cut(s) 622
BspACI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 197, 233, 1069
BspANI GGCC 6 cut(s) 246, 454, 525, 878, 968, 1123
BspCNI CTCAG 4 cut(s) 90, 138, 285, 297
BspDI ATCGAT 1 cut(s) 20
BspFNI CGCG 3 cut(s) 38, 233, 531
BspLI GGNNCC 2 cut(s) 114, 719
BspMAI CTGCAG 1 cut(s) 953
BspPI GGATC 4 cut(s) 817, 834, 1014, 1044
BspQI GCTCTTC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 112
BsrBI CCGCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 4 cut(s) 337, 563, 818, 1134
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 7 cut(s) 223, 622, 842, 843, 844, 932, 1125
BssNI GRCGYC 2 cut(s) 14, 168
BssSI CACGAG 1 cut(s) 389
BssT1I CCWWGG 2 cut(s) 622, 932
Bst2BI CACGAG 1 cut(s) 389
Bst4CI ACNGT 5 cut(s) 211, 227, 404, 680, 747
Bst6I CTCTTC 2 cut(s) 149, 266
BstACI GRCGYC 2 cut(s) 14, 168
BstAPI GCANNNNNTGC 1 cut(s) 948
BstC8I GCNNGC 2 cut(s) 117, 970
BstDEI CTNAG 4 cut(s) 98, 146, 272, 284
BstDSI CCRYGG 3 cut(s) 223, 622, 1125
BstENI CCTNNNNNAGG 1 cut(s) 803
BstF5I GGATG 2 cut(s) 108, 424
BstFNI CGCG 3 cut(s) 38, 233, 531
BstMAI GTCTC 1 cut(s) 392
BstMCI CGRYCG 1 cut(s) 554
BstMWI GCNNNNNNNGC 6 cut(s) 35, 55, 211, 346, 948, 1068
BstSCI CCNGG 3 cut(s) 192, 842, 843
BstSFI CTRYAG 1 cut(s) 949
BstUI CGCG 3 cut(s) 38, 233, 531
BstV1I GCAGC 3 cut(s) 64, 201, 361
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 2 cut(s) 463, 809
BstYI RGATCY 2 cut(s) 463, 809
Bsu15I ATCGAT 1 cut(s) 20
BsuI GTATCC 2 cut(s) 549, 801
BsuRI GGCC 6 cut(s) 246, 454, 525, 878, 968, 1123
BsuTUI ATCGAT 1 cut(s) 20
BtgI CCRYGG 3 cut(s) 223, 622, 1125
BtsCI GGATG 2 cut(s) 108, 424
BtsI GCAGTG 2 cut(s) 454, 501
BtsIMutI CAGTG 6 cut(s) 271, 454, 501, 676, 972, 1141
Cac8I GCNNGC 2 cut(s) 117, 970
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 4 cut(s) 244, 452, 966, 1122
Cfr9I CCCGGG 1 cut(s) 843
ClaI ATCGAT 1 cut(s) 20
CseI GACGC 1 cut(s) 520
Csp6I GTAC 5 cut(s) 64, 400, 556, 748, 912
CviAII CATG 4 cut(s) 517, 535, 623, 656
CviQI GTAC 5 cut(s) 64, 400, 556, 748, 912
DdeI CTNAG 4 cut(s) 98, 146, 272, 284
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 2 cut(s) 149, 266
EarI CTCTTC 2 cut(s) 149, 266
Ecl136II GAGCTC 3 cut(s) 259, 271, 736
Eco130I CCWWGG 2 cut(s) 622, 932
Eco24I GRGCYC 3 cut(s) 261, 273, 738
Eco53kI GAGCTC 3 cut(s) 259, 271, 736
Eco57I CTGAAG 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 843
EcoICRI GAGCTC 3 cut(s) 259, 271, 736
EcoNI CCTNNNNNAGG 1 cut(s) 803
EcoT14I CCWWGG 2 cut(s) 622, 932
EcoT38I GRGCYC 3 cut(s) 261, 273, 738
ErhI CCWWGG 2 cut(s) 622, 932
FaeI CATG 4 cut(s) 520, 538, 626, 659
FaqI GGGAC 1 cut(s) 640
FatI CATG 4 cut(s) 516, 534, 622, 655
FauI CCCGC 2 cut(s) 54, 83
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 8 cut(s) 27, 50, 53, 56, 198, 215, 234, 375
FokI GGATG 2 cut(s) 115, 431
FriOI GRGCYC 3 cut(s) 261, 273, 738
Fsp4HI GCNGC 8 cut(s) 27, 50, 53, 56, 198, 215, 234, 375
FspBI CTAG 3 cut(s) 461, 731, 933
GluI GCNGC 8 cut(s) 27, 50, 53, 56, 198, 215, 234, 375
HaeIII GGCC 6 cut(s) 246, 454, 525, 878, 968, 1123
HapII CCGG 4 cut(s) 116, 194, 542, 844
HgaI GACGC 1 cut(s) 520
Hin1I GRCGYC 2 cut(s) 14, 168
Hin1II CATG 4 cut(s) 520, 538, 626, 659
HincII GTYRAC 2 cut(s) 133, 292
HindII GTYRAC 2 cut(s) 133, 292
HinfI GANTC 1 cut(s) 288
HpaII CCGG 4 cut(s) 116, 194, 542, 844
HphI GGTGA 4 cut(s) 22, 199, 272, 556
Hpy166II GTNNAC 3 cut(s) 133, 181, 292
Hpy188I TCNGA 3 cut(s) 287, 826, 1088
Hpy188III TCNNGA 2 cut(s) 461, 903
Hpy8I GTNNAC 3 cut(s) 133, 181, 292
Hpy99I CGWCG 6 cut(s) 64, 134, 137, 170, 173, 176
HpyAV CCTTC 1 cut(s) 536
HpyCH4III ACNGT 5 cut(s) 211, 227, 404, 680, 747
HpyCH4IV ACGT 4 cut(s) 14, 129, 135, 168
HpyCH4V TGCA 6 cut(s) 217, 362, 407, 815, 951, 1104
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 55, 211, 346, 948, 1068
HpyF3I CTNAG 4 cut(s) 98, 146, 272, 284
HpySE526I ACGT 4 cut(s) 14, 129, 135, 168
Hsp92I GRCGYC 2 cut(s) 14, 168
Hsp92II CATG 4 cut(s) 520, 538, 626, 659
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
LguI GCTCTTC 1 cut(s) 266
Lsp1109I GCAGC 3 cut(s) 64, 201, 361
MaeI CTAG 3 cut(s) 461, 731, 933
MaeII ACGT 4 cut(s) 14, 129, 135, 168
MaeIII GTNAC 4 cut(s) 10, 227, 651, 725
MbiI CCGCTC 1 cut(s) 90
MboII GAAGA 6 cut(s) 34, 166, 253, 269, 494, 775
MfeI CAATTG 1 cut(s) 601
MflI RGATCY 2 cut(s) 463, 809
MhlI GDGCHC 3 cut(s) 261, 273, 738
MlsI TGGCCA 1 cut(s) 878
MluCI AATT 3 cut(s) 423, 485, 601
MluNI TGGCCA 1 cut(s) 878
MlyI GAGTC 1 cut(s) 297
Mox20I TGGCCA 1 cut(s) 878
MroNI GCCGGC 1 cut(s) 115
MscI TGGCCA 1 cut(s) 878
MseI TTAA 3 cut(s) 186, 488, 801
MslI CAYNNNNRTG 1 cut(s) 894
Msp20I TGGCCA 1 cut(s) 878
MspA1I CMGCKG 2 cut(s) 632, 779
MspI CCGG 4 cut(s) 116, 194, 542, 844
MspR9I CCNGG 3 cut(s) 194, 844, 845
MunI CAATTG 1 cut(s) 601
MvnI CGCG 3 cut(s) 38, 233, 531
MwoI GCNNNNNNNGC 6 cut(s) 35, 55, 211, 346, 948, 1068
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 3 cut(s) 194, 844, 845
NcoI CCATGG 1 cut(s) 622
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 4 cut(s) 520, 538, 626, 659
NlaIV GGNNCC 2 cut(s) 114, 719
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 3 cut(s) 10, 651, 725
PciSI GCTCTTC 1 cut(s) 266
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 174
PdiI GCCGGC 1 cut(s) 117
Pfl23II CGTACG 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 8 cut(s) 28, 51, 54, 57, 199, 216, 235, 376
Ple19I CGATCG 1 cut(s) 554
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
Psp124BI GAGCTC 3 cut(s) 261, 273, 738
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 114, 719
PspPI GGNCC 4 cut(s) 244, 452, 966, 1122
PstI CTGCAG 1 cut(s) 953
PsuI RGATCY 2 cut(s) 463, 809
PsyI GACNNNGTC 1 cut(s) 170
PvuI CGATCG 1 cut(s) 554
PvuII CAGCTG 2 cut(s) 632, 779
RsaI GTAC 5 cut(s) 65, 401, 557, 749, 913
RsaNI GTAC 5 cut(s) 64, 400, 556, 748, 912
RseI CAYNNNNRTG 1 cut(s) 894
SacI GAGCTC 3 cut(s) 261, 273, 738
SalI GTCGAC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 3 cut(s) 186, 488, 801
SatI GCNGC 8 cut(s) 27, 50, 53, 56, 198, 215, 234, 375
Sau96I GGNCC 4 cut(s) 244, 452, 966, 1122
ScaI AGTACT 1 cut(s) 913
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 3 cut(s) 194, 844, 845
SduI GDGCHC 3 cut(s) 261, 273, 738
SfcI CTRYAG 1 cut(s) 949
SgrDI CGTCGACG 1 cut(s) 131
SmaI CCCGGG 1 cut(s) 845
SmiMI CAYNNNNRTG 1 cut(s) 894
Sse9I AATT 3 cut(s) 423, 485, 601
SsiI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 197, 233, 1069
SspI AATATT 2 cut(s) 580, 1108
SspMI CTAG 3 cut(s) 461, 731, 933
SstI GAGCTC 3 cut(s) 261, 273, 738
StyD4I CCNGG 3 cut(s) 192, 842, 843
StyI CCWWGG 2 cut(s) 622, 932
TaaI ACNGT 5 cut(s) 211, 227, 404, 680, 747
TaiI ACGT 4 cut(s) 17, 132, 138, 171
TaqI TCGA 3 cut(s) 20, 125, 132
TasI AATT 3 cut(s) 423, 485, 601
TatI WGTACW 2 cut(s) 747, 911
TauI GCSGC 5 cut(s) 29, 52, 58, 200, 236
Tru1I TTAA 3 cut(s) 186, 488, 801
Tru9I TTAA 3 cut(s) 186, 488, 801
TscAI CASTG 6 cut(s) 271, 454, 501, 683, 979, 1141
TseFI GTSAC 3 cut(s) 10, 651, 725
TseI GCWGC 3 cut(s) 52, 214, 374
Tsp45I GTSAC 3 cut(s) 10, 651, 725
TspMI CCCGGG 1 cut(s) 843
TspRI CASTG 6 cut(s) 271, 454, 501, 683, 979, 1141
Tth111I GACNNNGTC 1 cut(s) 170
XagI CCTNNNNNAGG 1 cut(s) 803
XbaI TCTAGA 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 843
XmaJI CCTAGG 1 cut(s) 932
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 3 cut(s) 461, 731, 933
ZraI GACGTC 2 cut(s) 15, 169
ZrmI AGTACT 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.