Rw2G047900

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011381
Physical Location & Seq
Reverse (-)
75186697 .. 75189657
2961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G047900.1

Sequence Viewer

Length: 1167 bp
ATGGGGGCGGTGACGTCATCCATGGCGGCGAAGTTCGCGTTCTTCCCGCCGCACCCGCCGTCGTACGGGGTGGAGGAGGAGGAGGAGAGCGGGAAGCTGAGGATGACGGGAGTGCCGGCGAGGGCGAACGTCGACGTTTTGAAGCTGAAGACGAAGAGGGGAAACGACGTCGTCGCGGTTTACTTTAAGAACCCGGCGGCGAAGCTCACCGTTCTGCACTCCCACGGTAACGCAGCTGATCTGGGCCAGATGTATGAGCTCTTCAGCGAGCTCAGTCTTCACCTCAGAGTCAACTTAATAGGGTATGATTATTCTGGGTACGGACAATCTACTGGGAAGCCAAGTGAGCAAAACACTTATGCAGACATAGAAGCCGTGTATAGATGTCTCGTGGAGAAGTACGGTGCAAAGGAGGAGGATGTAATTTTATACGGGCAATCAGTTGGCAGTGGGCCTACTCTAGATCTGGCAACTCGCTTACCGAAATTGAGGGCAGTGGTTCTTCACAGTCCGATCATGTCTGGCCTTCGAGTCATGTATCCGGTGAAGCGATCGTACTGGTTTGACATTTACAAGAACATTGACAAAATACCGTTGGTCAAATGTCCTGTTTTGGTTATCCATGGGACAGCTGATGATGTTGTGGATTGGTCACATGGCAAGCAACTGTGGGAACACTGTAAGGAGAAGTACGAGCCATTGTGGATAAAAGGAGGGAACCATTGTGACTTGGAGCTCTACCCACAGTACATCAAGCATCTGAAGAAGTTTGTATCGGCCATTGAGAAGTTTCCACGTCTTAAAAATGGATCTGCACCAGTTCTTAAAAATGGATCTGCACCAGTTACGGATCAACTAGAAAAGCCCCGAGGCAGCACCGACATCAGGGAGAAACCTTGGTCCAACACAGATCATAAAGAGGTTTCTAGAACAAGTACTGACCATAGAGAAAACCCTAGGGTAAGCACAGACTGCAGAGAGAAGTCAAGGGCCAGCACTGATAAGAGAGATAGATCAAAAAAGATTATGGATCAACCCGAAAAGCCAAGTAATAGCGTGGAGCAGCCGGAGAAAGCAAGGAATAGCATTGACCGCTTTGGGGAGATGTTCAGATCAGTTGGCTTCTGCAATATTGATTGTTTTAGGCCCACGGCGACCAGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S9

Protein Analysis

388

Amino Acids

43.71

Weight (kDa)

8.92

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 67 - 174 1.6e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 72 - 173 9.5e-08 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30380 AT3G30380
fragaria_vesca FvH4_6g46130 FvH4_6g46130
malus_domestica MD09G1076700.v1.1 MD17G1067200.v1.1
prunus_persica Prupe.3G247400_v2.0.a1 Prupe.3G247400_v2.0.a1
pyrus_communis pycom09g00390 pycom17g06750
rosa_chinensis RchiOBHm_Chr2g0164581
rosa_laevigata RLG00000021467
rosa_multiflora Rmu_co8187214.1_g000001
rosa_roxburghii Rroxscaffold_2G00086470
rosa_rugosa Rorug02G0509300
rosa_samantha Rh2AG576900 Rh2BG588000 Rh2CG558800 Rh2DG598600
rosa_wichuraiana Rw2G047900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 17, 171
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 132
AccII CGCG 2 cut(s) 38, 176
AciI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 176, 197, 1093
AclWI GGATC 4 cut(s) 817, 841, 858, 1038
AcoI YGGCCR 1 cut(s) 777
AcuI CTGAAG 3 cut(s) 167, 247, 782
AcyI GRCGYC 2 cut(s) 14, 168
AfaI GTAC 7 cut(s) 65, 320, 401, 557, 692, 749, 937
AfiI CCNNNNNNNGG 3 cut(s) 65, 885, 1099
AgsI TTSAA 1 cut(s) 142
AjiI CACGTC 1 cut(s) 797
AluBI AGCT 8 cut(s) 97, 145, 205, 236, 259, 271, 632, 736
AluI AGCT 8 cut(s) 97, 145, 205, 236, 259, 271, 632, 736
Alw21I GWGCWC 3 cut(s) 261, 273, 738
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 4 cut(s) 817, 841, 858, 1038
Ama87I CYCGRG 1 cut(s) 867
AoxI GGCC 6 cut(s) 244, 452, 523, 777, 990, 1145
ApeKI GCWGC 3 cut(s) 233, 873, 1063
AspA2I CCTAGG 1 cut(s) 956
AspS9I GGNCC 5 cut(s) 244, 452, 900, 990, 1146
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 4 cut(s) 22, 199, 272, 556
AvaI CYCGRG 1 cut(s) 867
AvaII GGWCC 1 cut(s) 900
AvrII CCTAGG 1 cut(s) 956
BanII GRGCYC 3 cut(s) 261, 273, 738
BarI GAAGNNNNNNTAC 4 cut(s) 245, 277, 539, 571
BauI CACGAG 1 cut(s) 389
BbsI GAAGAC 2 cut(s) 155, 269
Bbv12I GWGCWC 3 cut(s) 261, 273, 738
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 245, 885, 1075
BceAI ACGGC 2 cut(s) 43, 359
BciVI GTATCC 1 cut(s) 549
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 4 cut(s) 461, 857, 927, 957
BfmI CTRYAG 1 cut(s) 973
BfuI GTATCC 1 cut(s) 549
BglII AGATCT 1 cut(s) 463
BisI GCNGC 6 cut(s) 27, 50, 198, 234, 874, 1064
BlnI CCTAGG 1 cut(s) 956
BlsI GCNGC 6 cut(s) 28, 51, 199, 235, 875, 1065
BmcAI AGTACT 1 cut(s) 937
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 1 cut(s) 900
BmeT110I CYCGRG 1 cut(s) 867
BmgBI CACGTC 1 cut(s) 797
BmgT120I GGNCC 5 cut(s) 244, 452, 900, 990, 1146
BmiI GGNNCC 1 cut(s) 719
BmrFI CCNGG 1 cut(s) 194
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 766
BmuI ACTGGG 1 cut(s) 342
BpiI GAAGAC 2 cut(s) 155, 269
Bpu10I CCTNAGC 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 2 cut(s) 14, 168
BsaJI CCNNGG 7 cut(s) 21, 223, 622, 868, 896, 956, 1149
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 885, 1099
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 5 cut(s) 337, 563, 818, 842, 1158
BseDI CCNNGG 7 cut(s) 21, 223, 622, 868, 896, 956, 1149
BseGI GGATG 3 cut(s) 17, 108, 424
BseLI CCNNNNNNNGG 3 cut(s) 65, 885, 1099
BseMII CTCAG 3 cut(s) 89, 286, 298
BseNI ACTGG 5 cut(s) 337, 563, 818, 842, 1158
BseRI GAGGAG 5 cut(s) 89, 92, 95, 98, 428
BseXI GCAGC 3 cut(s) 245, 885, 1075
BsgI GTGCAG 3 cut(s) 200, 798, 822
Bsh1236I CGCG 2 cut(s) 38, 176
Bsh1285I CGRYCG 1 cut(s) 554
BshFI GGCC 6 cut(s) 246, 454, 525, 779, 992, 1147
BsiEI CGRYCG 1 cut(s) 554
BsiHKAI GWGCWC 3 cut(s) 261, 273, 738
BsiHKCI CYCGRG 1 cut(s) 867
BsiSI CCGG 4 cut(s) 116, 194, 542, 1067
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 640
BslI CCNNNNNNNGG 3 cut(s) 65, 885, 1099
BsmAI GTCTC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 640
BsnI GGCC 6 cut(s) 246, 454, 525, 779, 992, 1147
BsoBI CYCGRG 1 cut(s) 867
Bsp1286I GDGCHC 3 cut(s) 261, 273, 738
Bsp19I CCATGG 2 cut(s) 21, 622
BspACI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 176, 197, 1093
BspANI GGCC 6 cut(s) 246, 454, 525, 779, 992, 1147
BspCNI CTCAG 3 cut(s) 90, 285, 297
BspFNI CGCG 2 cut(s) 38, 176
BspLI GGNNCC 1 cut(s) 719
BspMAI CTGCAG 1 cut(s) 977
BspPI GGATC 4 cut(s) 817, 841, 858, 1038
BspQI GCTCTTC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 5 cut(s) 337, 563, 818, 842, 1158
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 7 cut(s) 21, 223, 622, 868, 896, 956, 1149
BssNI GRCGYC 2 cut(s) 14, 168
BssSI CACGAG 1 cut(s) 389
BssT1I CCWWGG 4 cut(s) 21, 622, 896, 956
Bst2BI CACGAG 1 cut(s) 389
Bst4CI ACNGT 8 cut(s) 211, 227, 404, 509, 594, 669, 680, 747
Bst6I CTCTTC 2 cut(s) 149, 266
BstACI GRCGYC 2 cut(s) 14, 168
BstAPI GCANNNNNTGC 1 cut(s) 972
BstC8I GCNNGC 4 cut(s) 117, 269, 662, 994
BstDEI CTNAG 3 cut(s) 98, 272, 284
BstDSI CCRYGG 4 cut(s) 21, 223, 622, 1149
BstF5I GGATG 3 cut(s) 17, 108, 424
BstFNI CGCG 2 cut(s) 38, 176
BstMAI GTCTC 1 cut(s) 392
BstMCI CGRYCG 1 cut(s) 554
BstMWI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 972, 1092
BstSCI CCNGG 1 cut(s) 192
BstSFI CTRYAG 1 cut(s) 973
BstUI CGCG 2 cut(s) 38, 176
BstV1I GCAGC 3 cut(s) 245, 885, 1075
BstV2I GAAGAC 2 cut(s) 155, 269
BstX2I RGATCY 3 cut(s) 463, 809, 833
BstYI RGATCY 3 cut(s) 463, 809, 833
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 6 cut(s) 246, 454, 525, 779, 992, 1147
BtgI CCRYGG 4 cut(s) 21, 223, 622, 1149
BtrI CACGTC 1 cut(s) 797
BtsCI GGATG 3 cut(s) 17, 108, 424
BtsI GCAGTG 2 cut(s) 454, 501
BtsIMutI CAGTG 5 cut(s) 454, 501, 676, 996, 1165
Cac8I GCNNGC 4 cut(s) 117, 269, 662, 994
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 5 cut(s) 244, 452, 900, 990, 1146
Csp6I GTAC 7 cut(s) 64, 319, 400, 556, 691, 748, 936
CviAII CATG 5 cut(s) 22, 517, 535, 623, 656
CviQI GTAC 7 cut(s) 64, 319, 400, 556, 691, 748, 936
DdeI CTNAG 3 cut(s) 98, 272, 284
EaeI YGGCCR 1 cut(s) 777
Eam1104I CTCTTC 2 cut(s) 149, 266
EarI CTCTTC 2 cut(s) 149, 266
Ecl136II GAGCTC 3 cut(s) 259, 271, 736
Eco130I CCWWGG 4 cut(s) 21, 622, 896, 956
Eco24I GRGCYC 3 cut(s) 261, 273, 738
Eco47I GGWCC 1 cut(s) 900
Eco53kI GAGCTC 3 cut(s) 259, 271, 736
Eco57I CTGAAG 3 cut(s) 167, 247, 782
Eco88I CYCGRG 1 cut(s) 867
EcoICRI GAGCTC 3 cut(s) 259, 271, 736
EcoT14I CCWWGG 4 cut(s) 21, 622, 896, 956
EcoT38I GRGCYC 3 cut(s) 261, 273, 738
ErhI CCWWGG 4 cut(s) 21, 622, 896, 956
FaeI CATG 5 cut(s) 25, 520, 538, 626, 659
FaqI GGGAC 1 cut(s) 640
FatI CATG 5 cut(s) 21, 516, 534, 622, 655
FauI CCCGC 3 cut(s) 54, 63, 83
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 6 cut(s) 27, 50, 198, 234, 874, 1064
FokI GGATG 3 cut(s) 4, 115, 431
FriOI GRGCYC 3 cut(s) 261, 273, 738
Fsp4HI GCNGC 6 cut(s) 27, 50, 198, 234, 874, 1064
FspBI CTAG 4 cut(s) 461, 857, 927, 957
GluI GCNGC 6 cut(s) 27, 50, 198, 234, 874, 1064
HaeIII GGCC 6 cut(s) 246, 454, 525, 779, 992, 1147
HapII CCGG 4 cut(s) 116, 194, 542, 1067
Hin1I GRCGYC 2 cut(s) 14, 168
Hin1II CATG 5 cut(s) 25, 520, 538, 626, 659
HincII GTYRAC 2 cut(s) 133, 292
HindII GTYRAC 2 cut(s) 133, 292
HinfI GANTC 2 cut(s) 288, 531
HpaII CCGG 4 cut(s) 116, 194, 542, 1067
HphI GGTGA 4 cut(s) 22, 199, 272, 556
Hpy166II GTNNAC 3 cut(s) 133, 181, 292
Hpy188I TCNGA 4 cut(s) 287, 513, 762, 1112
Hpy188III TCNNGA 2 cut(s) 461, 927
Hpy8I GTNNAC 3 cut(s) 133, 181, 292
Hpy99I CGWCG 6 cut(s) 64, 134, 137, 170, 173, 176
HpyAV CCTTC 1 cut(s) 536
HpyCH4III ACNGT 8 cut(s) 211, 227, 404, 509, 594, 669, 680, 747
HpyCH4IV ACGT 5 cut(s) 14, 129, 135, 168, 796
HpyCH4V TGCA 7 cut(s) 217, 362, 407, 815, 839, 975, 1128
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 972, 1092
HpyF3I CTNAG 3 cut(s) 98, 272, 284
HpySE526I ACGT 5 cut(s) 14, 129, 135, 168, 796
Hsp92I GRCGYC 2 cut(s) 14, 168
Hsp92II CATG 5 cut(s) 25, 520, 538, 626, 659
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
LguI GCTCTTC 1 cut(s) 266
LmnI GCTCC 2 cut(s) 733, 1060
Lsp1109I GCAGC 3 cut(s) 245, 885, 1075
LweI GCATC 1 cut(s) 766
MaeI CTAG 4 cut(s) 461, 857, 927, 957
MaeII ACGT 5 cut(s) 14, 129, 135, 168, 796
MaeIII GTNAC 5 cut(s) 10, 227, 651, 725, 844
MbiI CCGCTC 1 cut(s) 90
MboII GAAGA 7 cut(s) 34, 160, 166, 253, 269, 494, 775
MflI RGATCY 3 cut(s) 463, 809, 833
MhlI GDGCHC 3 cut(s) 261, 273, 738
MluCI AATT 2 cut(s) 423, 485
MlyI GAGTC 2 cut(s) 297, 540
MmeI TCCRAC 1 cut(s) 927
MroNI GCCGGC 1 cut(s) 115
MseI TTAA 4 cut(s) 186, 296, 801, 825
MspA1I CMGCKG 2 cut(s) 236, 632
MspI CCGG 4 cut(s) 116, 194, 542, 1067
MspR9I CCNGG 1 cut(s) 194
MvnI CGCG 2 cut(s) 38, 176
MwoI GCNNNNNNNGC 5 cut(s) 35, 55, 346, 972, 1092
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 2 cut(s) 21, 622
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 5 cut(s) 25, 520, 538, 626, 659
NlaIV GGNNCC 1 cut(s) 719
NmuCI GTSAC 3 cut(s) 10, 651, 725
PciSI GCTCTTC 1 cut(s) 266
PdiI GCCGGC 1 cut(s) 117
Pfl23II CGTACG 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 6 cut(s) 28, 51, 199, 235, 875, 1065
Ple19I CGATCG 1 cut(s) 554
PleI GAGTC 2 cut(s) 296, 539
PpsI GAGTC 2 cut(s) 296, 539
Psp124BI GAGCTC 3 cut(s) 261, 273, 738
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 719
PspPI GGNCC 5 cut(s) 244, 452, 900, 990, 1146
PstI CTGCAG 1 cut(s) 977
PsuI RGATCY 3 cut(s) 463, 809, 833
PsyI GACNNNGTC 1 cut(s) 170
PvuI CGATCG 1 cut(s) 554
PvuII CAGCTG 2 cut(s) 236, 632
RsaI GTAC 7 cut(s) 65, 320, 401, 557, 692, 749, 937
RsaNI GTAC 7 cut(s) 64, 319, 400, 556, 691, 748, 936
SacI GAGCTC 3 cut(s) 261, 273, 738
SalI GTCGAC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 4 cut(s) 186, 296, 801, 825
SatI GCNGC 6 cut(s) 27, 50, 198, 234, 874, 1064
Sau96I GGNCC 5 cut(s) 244, 452, 900, 990, 1146
ScaI AGTACT 1 cut(s) 937
SchI GAGTC 2 cut(s) 297, 540
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 3 cut(s) 261, 273, 738
SfaNI GCATC 1 cut(s) 766
SfcI CTRYAG 1 cut(s) 973
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 1 cut(s) 900
Sse9I AATT 2 cut(s) 423, 485
SsiI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 176, 197, 1093
SspI AATATT 1 cut(s) 1132
SspMI CTAG 4 cut(s) 461, 857, 927, 957
SstI GAGCTC 3 cut(s) 261, 273, 738
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 4 cut(s) 21, 622, 896, 956
TaaI ACNGT 8 cut(s) 211, 227, 404, 509, 594, 669, 680, 747
TaiI ACGT 5 cut(s) 17, 132, 138, 171, 799
TaqI TCGA 2 cut(s) 132, 529
TasI AATT 2 cut(s) 423, 485
TatI WGTACW 2 cut(s) 747, 935
TauI GCSGC 3 cut(s) 29, 52, 200
Tru1I TTAA 4 cut(s) 186, 296, 801, 825
Tru9I TTAA 4 cut(s) 186, 296, 801, 825
TscAI CASTG 5 cut(s) 454, 501, 683, 1003, 1165
TseFI GTSAC 3 cut(s) 10, 651, 725
TseI GCWGC 3 cut(s) 233, 873, 1063
Tsp45I GTSAC 3 cut(s) 10, 651, 725
TspGWI ACGGA 2 cut(s) 336, 863
TspRI CASTG 5 cut(s) 454, 501, 683, 1003, 1165
Tth111I GACNNNGTC 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 900
XbaI TCTAGA 2 cut(s) 460, 926
XmaJI CCTAGG 1 cut(s) 956
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 4 cut(s) 461, 857, 927, 957
ZraI GACGTC 2 cut(s) 15, 169
ZrmI AGTACT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.