AT3G44190

Apoptosis-inducing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007414
Physical Location & Seq
Reverse (-)
15901714 .. 15903628
1915 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44190.2

Sequence Viewer

Length: 1104 bp
ATGGAAAAAACAGAATCTGTATCAGGAAAGGGGAAGAGAGTGATTGTTATCGGCGGCGGAATCGCCGGCTCTCTCGCTTCGAAATTTCTTCAATTCGATTCCGATCTCACCCTCATTGATCCGAAGGAGTATTTTGAGATTATTTGGGCAAGTTTAAGGTCAATGGTAGAGCCAGCTTTTGCTGAAAGATCGGTGATAAACCACAAGAAGTATTTACAAAATGGTCGTGTTGTGACTTCTCCAGCGGTTAATATCACAGACACCGATGTTTTGACCGCGGATGGTCTTGTCTTTGGGTATGATTATCTTGTTATTGCTACTGGTCACAATGATGTGCTTCCTAAAACTAGGCAAGAGAAGTTATCTCAATACCAAACAGAACATGAGAAGATCAAGTCATCTGAGTCGATTCTGATTGTTGGTGGAGGTCCGTCGGGTGTGGAGCTCGCTGCGGAGATAGCTGTCGATTTCCCTGAGAAAAAGGTTACTATAGTACACAATGGACCGAGATTGCTGGAATTTGTTGGTCAGAAAGCTGCAGACAAGGCATTAGACTGGATGAAGTCCAAGAGAGTTGAAGTGATATTGAACCAAAGGGTTGATTTGAGTTCAGCTTCAGATGGGAGTAAAACGTACCGAACATCGGGTGGAGAGACGATACACGCGGATTGTCACTTCCTCTGTACTGGAAAACCTTTGTCTTCAGAATGGCTAAAAGGAACTGTTCTGAAAGATAACTTGGATGGCAAAGGAAGGCTTATGGTCGATGAGTACTTGAGAGTTAAGGGTCGCAAGAATGTATTCGCTATTGGTGATATTACTGATGTCCCGGAGATGAAACAAGGCTACATTGCTGAGAAACACGCCTCTGTAGCTACAAAGAACATAAAGTTGTTGATGTCTGGTGGGAACGAGAAGAAGATGTCGACTTACAAGCCTGGTCCGGACATTGCCATAATATCTTTAGGAAGAAAGGACTCGGTTGCTCAGTTCCCATTCATGACAGTCTCGGGTTGCATACCTGGTTTGATCAAATCTAAGGATCTGTTTGTCGGGAAGACGAGGAAGGCAAGAGGTCTGGATCCTAACCTTGTGGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pyr_redox_2

Protein Analysis

367

Amino Acids

40.08

Weight (kDa)

8.94

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 31 - 231 2.3e-30 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 43 - 214 4.1e-06 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 83 - 151 4.2e-09 Pyridine nucleotide-disulphide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 926
AccII CGCG 2 cut(s) 278, 665
AccIII TCCGGA 1 cut(s) 943
AciI CCGC 7 cut(s) 54, 57, 245, 276, 278, 452, 665
AclWI GGATC 4 cut(s) 113, 1050, 1076, 1089
AcsI RAATTY 2 cut(s) 83, 518
AcuI CTGAAG 2 cut(s) 600, 687
AfaI GTAC 4 cut(s) 495, 635, 685, 773
AfiI CCNNNNNNNGG 1 cut(s) 643
AgsI TTSAA 3 cut(s) 92, 578, 589
AjnI CCWGG 2 cut(s) 937, 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 722, 754
AluBI AGCT 6 cut(s) 176, 445, 461, 536, 614, 875
AluI AGCT 6 cut(s) 176, 445, 461, 536, 614, 875
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 2 cut(s) 647, 1012
AlwI GGATC 4 cut(s) 113, 1050, 1076, 1089
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 1009
Aor13HI TCCGGA 1 cut(s) 943
ApeKI GCWGC 2 cut(s) 449, 536
ApoI RAATTY 2 cut(s) 83, 518
Asp700I GAANNNNTTC 1 cut(s) 800
AspS9I GGNCC 3 cut(s) 428, 503, 941
AsuC2I CCSGG 1 cut(s) 830
AsuHPI GGTGA 3 cut(s) 100, 205, 824
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 1009
AvaII GGWCC 3 cut(s) 428, 503, 941
BamHI GGATCC 1 cut(s) 1081
BanII GRGCYC 1 cut(s) 447
BbsI GAAGAC 2 cut(s) 693, 1064
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 2 cut(s) 436, 523
BccI CCATC 3 cut(s) 275, 614, 737
BciT130I CCWGG 2 cut(s) 939, 1023
BclI TGATCA 1 cut(s) 1029
BcnI CCSGG 1 cut(s) 830
BcoDI GTCTC 2 cut(s) 647, 1012
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 3 cut(s) 489, 537, 870
BisI GCNGC 3 cut(s) 55, 450, 537
BlsI GCNGC 3 cut(s) 56, 451, 538
BmcAI AGTACT 1 cut(s) 773
Bme1390I CCNGG 3 cut(s) 830, 939, 1023
Bme18I GGWCC 3 cut(s) 428, 503, 941
BmeT110I CYCGRG 1 cut(s) 1009
BmgT120I GGNCC 3 cut(s) 428, 503, 941
BmiI GGNNCC 2 cut(s) 1083, 1098
BmrFI CCNGG 3 cut(s) 830, 939, 1023
BpiI GAAGAC 2 cut(s) 693, 1064
BpmI CTGGAG 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 796
BpuMI CCSGG 1 cut(s) 830
BsaBI GATNNNNATC 2 cut(s) 47, 102
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 943
Bsc4I CCNNNNNNNGG 1 cut(s) 643
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 3 cut(s) 325, 560, 691
Bse3DI GCAATG 2 cut(s) 849, 948
Bse8I GATNNNNATC 2 cut(s) 47, 102
BseAI TCCGGA 1 cut(s) 943
BseBI CCWGG 2 cut(s) 939, 1023
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 3 cut(s) 286, 564, 748
BseJI GATNNNNATC 2 cut(s) 47, 102
BseLI CCNNNNNNNGG 1 cut(s) 643
BseMI GCAATG 2 cut(s) 849, 948
BseMII CTCAG 4 cut(s) 393, 465, 846, 1001
BseNI ACTGG 3 cut(s) 325, 560, 691
BseXI GCAGC 2 cut(s) 436, 523
Bsh1236I CGCG 2 cut(s) 278, 665
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 1009
BsiSI CCGG 3 cut(s) 66, 830, 944
BslFI GGGAC 1 cut(s) 812
BslI CCNNNNNNNGG 1 cut(s) 643
BsmAI GTCTC 2 cut(s) 647, 1012
BsmBI CGTCTC 1 cut(s) 647
BsmFI GGGAC 1 cut(s) 812
BsoBI CYCGRG 1 cut(s) 1009
Bsp119I TTCGAA 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 447
Bsp13I TCCGGA 1 cut(s) 943
Bsp143I GATC 7 cut(s) 103, 118, 188, 390, 1029, 1042, 1081
BspACI CCGC 7 cut(s) 54, 57, 245, 276, 278, 452, 665
BspCNI CTCAG 4 cut(s) 394, 466, 847, 1000
BspEI TCCGGA 1 cut(s) 943
BspFNI CGCG 2 cut(s) 278, 665
BspHI TCATGA 1 cut(s) 999
BspLI GGNNCC 2 cut(s) 1083, 1098
BspMAI CTGCAG 1 cut(s) 541
BspPI GGATC 4 cut(s) 113, 1050, 1076, 1089
BspT104I TTCGAA 1 cut(s) 80
BsrDI GCAATG 2 cut(s) 849, 948
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 3 cut(s) 325, 560, 691
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 7 cut(s) 103, 118, 188, 390, 1029, 1042, 1081
Bst2UI CCWGG 2 cut(s) 939, 1023
Bst4CI ACNGT 2 cut(s) 724, 1006
Bst6I CTCTTC 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 3 cut(s) 67, 174, 447
BstDEI CTNAG 5 cut(s) 402, 474, 855, 987, 1038
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 3 cut(s) 286, 564, 748
BstFNI CGCG 2 cut(s) 278, 665
BstKTI GATC 7 cut(s) 106, 121, 191, 393, 1032, 1045, 1084
BstMAI GTCTC 2 cut(s) 647, 1012
BstMBI GATC 7 cut(s) 103, 118, 188, 390, 1029, 1042, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 458, 545, 872
BstNI CCWGG 2 cut(s) 939, 1023
BstSCI CCNGG 3 cut(s) 828, 937, 1021
BstSFI CTRYAG 3 cut(s) 489, 537, 870
BstUI CGCG 2 cut(s) 278, 665
BstV1I GCAGC 2 cut(s) 436, 523
BstV2I GAAGAC 2 cut(s) 693, 1064
BstX2I RGATCY 2 cut(s) 1042, 1081
BstYI RGATCY 2 cut(s) 1042, 1081
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 3 cut(s) 286, 564, 748
Cac8I GCNNGC 3 cut(s) 67, 174, 447
CaiI CAGNNNCTG 1 cut(s) 17
CciI TCATGA 1 cut(s) 999
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 428, 503, 941
Cfr42I CCGCGG 1 cut(s) 279
CsiI ACCWGGT 1 cut(s) 1021
Csp6I GTAC 4 cut(s) 494, 634, 684, 772
CviAII CATG 2 cut(s) 383, 1000
CviQI GTAC 4 cut(s) 494, 634, 684, 772
DdeI CTNAG 5 cut(s) 402, 474, 855, 987, 1038
DpnI GATC 7 cut(s) 105, 120, 190, 392, 1031, 1044, 1083
DpnII GATC 7 cut(s) 103, 118, 188, 390, 1029, 1042, 1081
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 72
Ecl136II GAGCTC 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 447
Eco47I GGWCC 3 cut(s) 428, 503, 941
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 600, 687
Eco88I CYCGRG 1 cut(s) 1009
EcoICRI GAGCTC 1 cut(s) 445
EcoRII CCWGG 2 cut(s) 937, 1021
EcoT38I GRGCYC 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 647
FaeI CATG 2 cut(s) 386, 1003
FaiI YATR 8 cut(s) 300, 384, 491, 761, 887, 956, 1001, 1019
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FaqI GGGAC 1 cut(s) 812
FatI CATG 2 cut(s) 382, 999
FbaI TGATCA 1 cut(s) 1029
FblI GTMKAC 1 cut(s) 926
Fnu4HI GCNGC 3 cut(s) 55, 450, 537
FokI GGATG 3 cut(s) 293, 571, 755
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 3 cut(s) 55, 450, 537
FspBI CTAG 1 cut(s) 348
GluI GCNGC 3 cut(s) 55, 450, 537
GsuI CTGGAG 1 cut(s) 225
HapII CCGG 3 cut(s) 66, 830, 944
Hin1II CATG 2 cut(s) 386, 1003
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HinfI GANTC 6 cut(s) 14, 60, 98, 404, 409, 977
HpaII CCGG 3 cut(s) 66, 830, 944
HphI GGTGA 3 cut(s) 100, 205, 824
Hpy166II GTNNAC 2 cut(s) 496, 927
Hpy188I TCNGA 8 cut(s) 103, 123, 403, 414, 531, 619, 706, 729
Hpy188III TCNNGA 5 cut(s) 24, 944, 1000, 1054, 1079
Hpy8I GTNNAC 2 cut(s) 496, 927
Hpy99I CGWCG 1 cut(s) 436
HpyAV CCTTC 3 cut(s) 118, 747, 1060
HpyCH4III ACNGT 2 cut(s) 724, 1006
HpyCH4IV ACGT 1 cut(s) 632
HpyCH4V TGCA 2 cut(s) 539, 1017
HpyF10VI GCNNNNNNNGC 3 cut(s) 458, 545, 872
HpyF3I CTNAG 5 cut(s) 402, 474, 855, 987, 1038
HpySE526I ACGT 1 cut(s) 632
Hsp92II CATG 2 cut(s) 386, 1003
Kpn2I TCCGGA 1 cut(s) 943
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 1029
KspI CCGCGG 1 cut(s) 279
Kzo9I GATC 7 cut(s) 103, 118, 188, 390, 1029, 1042, 1081
LmnI GCTCC 2 cut(s) 442, 1096
Lsp1109I GCAGC 2 cut(s) 436, 523
MabI ACCWGGT 1 cut(s) 1021
MaeI CTAG 1 cut(s) 348
MaeII ACGT 1 cut(s) 632
MaeIII GTNAC 4 cut(s) 232, 323, 484, 671
MalI GATC 7 cut(s) 105, 120, 190, 392, 1031, 1044, 1083
MboI GATC 7 cut(s) 103, 118, 188, 390, 1029, 1042, 1081
MboII GAAGA 8 cut(s) 46, 80, 400, 693, 928, 931, 981, 1069
MflI RGATCY 2 cut(s) 1042, 1081
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 3 cut(s) 83, 92, 518
MlyI GAGTC 2 cut(s) 413, 971
MnlI CCTC 6 cut(s) 122, 419, 689, 877, 1056, 1067
MroI TCCGGA 1 cut(s) 943
MroNI GCCGGC 1 cut(s) 65
MroXI GAANNNNTTC 1 cut(s) 800
MseI TTAA 3 cut(s) 155, 249, 783
MslI CAYNNNNRTG 1 cut(s) 330
MspA1I CMGCKG 2 cut(s) 245, 278
MspI CCGG 3 cut(s) 66, 830, 944
MspR9I CCNGG 3 cut(s) 830, 939, 1023
MvaI CCWGG 2 cut(s) 939, 1023
MvnI CGCG 2 cut(s) 278, 665
MwoI GCNNNNNNNGC 3 cut(s) 458, 545, 872
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 1 cut(s) 830
NdeII GATC 7 cut(s) 103, 118, 188, 390, 1029, 1042, 1081
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 2 cut(s) 386, 1003
NlaIV GGNNCC 2 cut(s) 1083, 1098
NmuCI GTSAC 3 cut(s) 232, 323, 671
NspV TTCGAA 1 cut(s) 80
PagI TCATGA 1 cut(s) 999
PdiI GCCGGC 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 800
PfeI GAWTC 4 cut(s) 14, 60, 98, 409
PfoI TCCNGGA 1 cut(s) 828
PkrI GCNGC 3 cut(s) 56, 451, 538
PleI GAGTC 2 cut(s) 412, 971
PpsI GAGTC 2 cut(s) 412, 971
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 937, 1021
PspGI CCWGG 2 cut(s) 937, 1021
PspN4I GGNNCC 2 cut(s) 1083, 1098
PspPI GGNCC 3 cut(s) 428, 503, 941
PstI CTGCAG 1 cut(s) 541
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 2 cut(s) 1042, 1081
RsaI GTAC 4 cut(s) 495, 635, 685, 773
RsaNI GTAC 4 cut(s) 494, 634, 684, 772
RseI CAYNNNNRTG 1 cut(s) 330
SacI GAGCTC 1 cut(s) 447
SacII CCGCGG 1 cut(s) 279
SalI GTCGAC 1 cut(s) 925
SaqAI TTAA 3 cut(s) 155, 249, 783
SatI GCNGC 3 cut(s) 55, 450, 537
Sau3AI GATC 7 cut(s) 103, 118, 188, 390, 1029, 1042, 1081
Sau96I GGNCC 3 cut(s) 428, 503, 941
ScaI AGTACT 1 cut(s) 773
SchI GAGTC 2 cut(s) 413, 971
ScrFI CCNGG 3 cut(s) 830, 939, 1023
SduI GDGCHC 1 cut(s) 447
SexAI ACCWGGT 1 cut(s) 1021
SfcI CTRYAG 3 cut(s) 489, 537, 870
Sfr303I CCGCGG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 279
SinI GGWCC 3 cut(s) 428, 503, 941
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 775
SmoI CTYRAG 1 cut(s) 775
Sse9I AATT 3 cut(s) 83, 92, 518
SsiI CCGC 7 cut(s) 54, 57, 245, 276, 278, 452, 665
SspMI CTAG 1 cut(s) 348
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 3 cut(s) 828, 937, 1021
TaaI ACNGT 2 cut(s) 724, 1006
TaiI ACGT 1 cut(s) 635
TaqI TCGA 6 cut(s) 80, 96, 407, 465, 765, 926
TaqII GACCGA 1 cut(s) 520
TasI AATT 3 cut(s) 83, 92, 518
TatI WGTACW 3 cut(s) 493, 683, 771
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 4 cut(s) 14, 60, 98, 409
Tru1I TTAA 3 cut(s) 155, 249, 783
Tru9I TTAA 3 cut(s) 155, 249, 783
TseFI GTSAC 3 cut(s) 232, 323, 671
TseI GCWGC 2 cut(s) 449, 536
Tsp45I GTSAC 3 cut(s) 232, 323, 671
TspDTI ATGAA 3 cut(s) 575, 851, 988
TspGWI ACGGA 1 cut(s) 420
VpaK11BI GGWCC 3 cut(s) 428, 503, 941
XapI RAATTY 2 cut(s) 83, 518
XmiI GTMKAC 1 cut(s) 926
XmnI GAANNNNTTC 1 cut(s) 800
XspI CTAG 1 cut(s) 348
ZrmI AGTACT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.