AT3G47480

Secreted protein acidic and rich in cysteine Ca binding region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016496
Physical Location & Seq
Reverse (-)
17496085 .. 17497164
1080 bp
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UTR
Exon/CDS
Intron
AT3G47480.1

Sequence Viewer

Length: 552 bp
ATGGAGGATTCGTCTCTTCTTAGCCCAATCTCTCTCCTCACCATTGTCATCTTCTTATTCATTCTCAATCTCATGATGATAATTCAAGACTTCTCTTCTTCTTTTCCCTTTCGTTTCCATCTCTTCTTCTCTAATGCCTACATTCTCTTCACCTCAATAAGAAACAATAAGCAAAATACCGAGCTCCCAATCATAAAGAAAGTTGTTGTCCCAAATCGAGCAGATATCAAAACGTCCGTGGAAGAAGTCAAGGCGATAATCGATGACTCGGAAGCTTTATACGAATGTTTGATTGAAGAAGGAGAAGAGTACTTGCTTGAGAAGAATGAGATGATGGGTAAGGAGATAGTTAAAGAGGCGTTTAGGTTATTTGATGAGAATCAGGATGGGTTTATAGATGAAAATGAGTTAAAACATGTGTTGAGTTTGTTAGGATACGATGAGTGTACAAAGATGGAGTGTAGGAAGATGGTTAAGGTTTATGATGAGAATAGAGATGGAAAAATTGATTTTTATGAGTTTGTGAAGCTTATAGAGAAGAGCTTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1

Protein Analysis

183

Amino Acids

21.46

Weight (kDa)

4.64

Isoelectric Point (pI)

51.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_FSTL1 PF23564 106 - 182 7.5e-06 Follistatin-related protein 1, EF-hand domain pair
EF-hand_1 PF00036 117 - 142 4.6e-08 EF hand domain
EF-hand_6 PF13405 118 - 145 1.1e-07 EF-hand domain
EF-hand_7 PF13499 118 - 178 2.9e-13 EF-hand domain pair
EF-hand_5 PF13202 118 - 140 8.7e-06 EF hand
EF-hand_8 PF13833 129 - 179 2.8e-08 EF-hand domain pair
EF-hand_1 PF00036 153 - 180 9.6e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47480
fragaria_vesca FvH4_5g14810
malus_domestica MD06G1194700.v1.1 MD14G1201700.v1.1
prunus_persica Prupe.5G194800_v2.0.a1
pyrus_communis pycom14g16710
rosa_chinensis RchiOBHm_Chr7g0181581
rosa_multiflora Rmu_sc0000988.1_g000019
rosa_roxburghii Rroxscaffold_3G00271880
rosa_samantha Rh7AG044600 Rh7BG043600 Rh7CG045400 Rh7DG044200
rosa_wichuraiana Rw7G003700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 2 cut(s) 311, 448
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 2 cut(s) 86, 296
AluBI AGCT 4 cut(s) 184, 275, 529, 543
AluI AGCT 4 cut(s) 184, 275, 529, 543
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 18
AsuHPI GGTGA 2 cut(s) 31, 142
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 186
BccI CCATC 6 cut(s) 126, 328, 380, 448, 463, 491
BciVI GTATCC 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 18
BfuI GTATCC 1 cut(s) 428
BmcAI AGTACT 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 338
Bsa29I ATCGAT 1 cut(s) 261
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 237
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 378
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 1 cut(s) 391
BseJI GATNNNNATC 1 cut(s) 378
BseRI GAGGAG 1 cut(s) 26
BshVI ATCGAT 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 186
BslFI GGGAC 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 18
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 446
BspDI ATCGAT 1 cut(s) 261
BspHI TCATGA 1 cut(s) 72
BspQI GCTCTTC 1 cut(s) 533
BsrGI TGTACA 1 cut(s) 446
BssECI CCNNGG 1 cut(s) 237
Bst6I CTCTTC 6 cut(s) 21, 100, 128, 152, 300, 533
BstAUI TGTACA 1 cut(s) 446
BstDEI CTNAG 1 cut(s) 20
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 1 cut(s) 391
BstMAI GTCTC 1 cut(s) 18
BstNSI RCATGY 1 cut(s) 419
Bsu15I ATCGAT 1 cut(s) 261
BsuI GTATCC 1 cut(s) 428
BsuTUI ATCGAT 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 1 cut(s) 391
CciI TCATGA 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 261
Csp6I GTAC 2 cut(s) 310, 447
CviAII CATG 2 cut(s) 73, 416
CviJI RGCY 5 cut(s) 24, 184, 275, 529, 543
CviKI_1 RGCY 5 cut(s) 24, 184, 275, 529, 543
CviQI GTAC 2 cut(s) 310, 447
DdeI CTNAG 1 cut(s) 20
Eam1104I CTCTTC 6 cut(s) 21, 100, 128, 152, 300, 533
EarI CTCTTC 6 cut(s) 21, 100, 128, 152, 300, 533
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 184
EcoRV GATATC 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 2 cut(s) 76, 419
FaiI YATR 8 cut(s) 74, 194, 280, 395, 417, 483, 516, 533
FaqI GGGAC 1 cut(s) 194
FatI CATG 2 cut(s) 72, 415
FokI GGATG 1 cut(s) 398
FriOI GRGCYC 1 cut(s) 186
Hin1II CATG 2 cut(s) 76, 419
HindIII AAGCTT 2 cut(s) 273, 527
HinfI GANTC 3 cut(s) 8, 266, 379
HphI GGTGA 2 cut(s) 31, 142
Hpy166II GTNNAC 1 cut(s) 447
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 4 cut(s) 73, 86, 383, 549
Hpy8I GTNNAC 1 cut(s) 447
HpyAV CCTTC 1 cut(s) 293
HpyCH4IV ACGT 1 cut(s) 233
HpyF3I CTNAG 1 cut(s) 20
HpySE526I ACGT 1 cut(s) 233
Hsp92II CATG 2 cut(s) 76, 419
LguI GCTCTTC 1 cut(s) 533
LmnI GCTCC 1 cut(s) 189
LpnPI CCDG 1 cut(s) 368
MaeII ACGT 1 cut(s) 233
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 2 cut(s) 81, 504
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 3 cut(s) 47, 163, 349
MseI TTAA 3 cut(s) 351, 410, 474
NlaIII CATG 2 cut(s) 76, 419
NspI RCATGY 1 cut(s) 419
PagI TCATGA 1 cut(s) 72
PciI ACATGT 1 cut(s) 415
PciSI GCTCTTC 1 cut(s) 533
PfeI GAWTC 2 cut(s) 8, 379
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PscI ACATGT 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 186
RsaI GTAC 2 cut(s) 311, 448
RsaNI GTAC 2 cut(s) 310, 447
SacI GAGCTC 1 cut(s) 186
SapI GCTCTTC 1 cut(s) 533
SaqAI TTAA 3 cut(s) 351, 410, 474
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 1 cut(s) 260
SduI GDGCHC 1 cut(s) 186
SetI ASST 8 cut(s) 155, 186, 236, 277, 368, 480, 531, 545
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 2 cut(s) 81, 504
SstI GAGCTC 1 cut(s) 186
TaiI ACGT 1 cut(s) 236
TaqI TCGA 2 cut(s) 217, 261
TasI AATT 2 cut(s) 81, 504
TatI WGTACW 2 cut(s) 309, 446
TfiI GAWTC 2 cut(s) 8, 379
Tru1I TTAA 3 cut(s) 351, 410, 474
Tru9I TTAA 3 cut(s) 351, 410, 474
TspDTI ATGAA 2 cut(s) 49, 414
TspGWI ACGGA 1 cut(s) 226
XceI RCATGY 1 cut(s) 419
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.