FvH4_5g14810

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016496
Physical Location & Seq
Reverse (-)
8409361 .. 8410330
970 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14810.t1

Sequence Viewer

Length: 591 bp
ATGGAGAAGACATTCATGGATGATGCAATTGCCAGCAGCTCCCTTGCACTTGTTGGGGTTCTGTTTTTCATAATGATTATTAAGTGGCTCATTGTGCAATTCTTTCTACACTTGTCAAGTTTGAGGTATGCGCTTCAATTCTTTGTAAGTTACTTGCTTAATACTGGTCTGCGCAATGTTTGCCGCAATAATCAGACCAAGATCACAACCCAGCAAGCTTGCATTTACAAGACAGTAGGTGTCGAACAGAACACGAAAGACTTGGGTGACACGAAACTGTGTTTAGGAGAGTTGGCAGCGGTTATGGAGAACCTGGGAAGTTTATGTGATGATAATGGGGCTAAGGAGATGGCTGACTTGCTTGAGGAGGAACCCAGCTTGGAGGAAGTGAAAGACGCCTTCCATTTGTTTGATGAAAATGCAGATGGGTTCATCGATGCAGGGGAGATCAAGAAGGTTCTCCATGTATTAGGTTTCATCCAAGCTTCGGAGTCAGAATGCAAAAGAATGATCAAGTCCTTCGATCAAAATGGAGATGGACGTATAGACTTTGAGGAATTTGTTAAGCTCATGGAAGATAGCTTTTGCTGA

Protein Analysis

197

Amino Acids

22.09

Weight (kDa)

4.56

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 128 - 191 3.2e-15 EF-hand domain pair
EF-hand_6 PF13405 129 - 158 4.1e-06 EF-hand domain
EF-hand_8 PF13833 142 - 193 1.4e-08 EF-hand domain pair
EF-hand_1 PF00036 166 - 192 5.9e-09 EF hand domain
EF-hand_5 PF13202 171 - 191 7.6e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47480
fragaria_vesca FvH4_5g14810
malus_domestica MD06G1194700.v1.1 MD14G1201700.v1.1
prunus_persica Prupe.5G194800_v2.0.a1
pyrus_communis pycom14g16710
rosa_chinensis RchiOBHm_Chr7g0181581
rosa_multiflora Rmu_sc0000988.1_g000019
rosa_roxburghii Rroxscaffold_3G00271880
rosa_samantha Rh7AG044600 Rh7BG043600 Rh7CG045400 Rh7DG044200
rosa_wichuraiana Rw7G003700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 173
AciI CCGC 2 cut(s) 184, 299
AcsI RAATTY 1 cut(s) 557
AcyI GRCGYC 1 cut(s) 396
AfiI CCNNNNNNNGG 1 cut(s) 487
AgsI TTSAA 1 cut(s) 137
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 6 cut(s) 39, 218, 378, 485, 568, 582
AluI AGCT 6 cut(s) 39, 218, 378, 485, 568, 582
ApeKI GCWGC 2 cut(s) 36, 296
ApoI RAATTY 1 cut(s) 557
Asp700I GAANNNNTTC 1 cut(s) 11
AspLEI GCGC 2 cut(s) 133, 174
AsuHPI GGTGA 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 48, 308
BccI CCATC 3 cut(s) 343, 419, 530
BciT130I CCWGG 1 cut(s) 314
BclI TGATCA 1 cut(s) 510
BisI GCNGC 3 cut(s) 37, 184, 297
BlsI GCNGC 3 cut(s) 38, 185, 298
Bme1390I CCNGG 1 cut(s) 314
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 1 cut(s) 314
BmsI GCATC 2 cut(s) 13, 427
BpiI GAAGAC 1 cut(s) 14
Bpu10I CCTNAGC 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 383
Bsa29I ATCGAT 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 313
Bsc4I CCNNNNNNNGG 1 cut(s) 487
Bse1I ACTGG 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 181
BseBI CCWGG 1 cut(s) 314
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 2 cut(s) 25, 477
BseLI CCNNNNNNNGG 1 cut(s) 487
BseMI GCAATG 1 cut(s) 181
BseNI ACTGG 1 cut(s) 169
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 48, 308
BseYI CCCAGC 2 cut(s) 210, 374
BshVI ATCGAT 1 cut(s) 435
BslI CCNNNNNNNGG 1 cut(s) 487
BsmI GAATGC 1 cut(s) 503
Bsp143I GATC 4 cut(s) 201, 447, 510, 523
BspACI CCGC 2 cut(s) 184, 299
BspDI ATCGAT 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 181
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 4 cut(s) 201, 447, 510, 523
BssNI GRCGYC 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 235, 279
BstACI GRCGYC 1 cut(s) 396
BstAPI GCANNNNNTGC 1 cut(s) 180
BstC8I GCNNGC 3 cut(s) 34, 216, 220
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 2 cut(s) 25, 477
BstHHI GCGC 2 cut(s) 133, 174
BstKTI GATC 4 cut(s) 204, 450, 513, 526
BstMBI GATC 4 cut(s) 201, 447, 510, 523
BstMWI GCNNNNNNNGC 2 cut(s) 94, 180
BstNI CCWGG 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 312
BstV1I GCAGC 2 cut(s) 48, 308
BstV2I GAAGAC 1 cut(s) 14
Bsu15I ATCGAT 1 cut(s) 435
BsuTUI ATCGAT 1 cut(s) 435
BtsCI GGATG 2 cut(s) 25, 477
Cac8I GCNNGC 3 cut(s) 34, 216, 220
CfoI GCGC 2 cut(s) 133, 174
ClaI ATCGAT 1 cut(s) 435
CseI GACGC 1 cut(s) 404
CviAII CATG 3 cut(s) 16, 464, 571
CviJI RGCY 9 cut(s) 39, 88, 218, 341, 353, 378, 485, 568, 582
CviKI_1 RGCY 9 cut(s) 39, 88, 218, 341, 353, 378, 485, 568, 582
DdeI CTNAG 1 cut(s) 342
DpnI GATC 4 cut(s) 203, 449, 512, 525
DpnII GATC 4 cut(s) 201, 447, 510, 523
EcoRII CCWGG 1 cut(s) 312
FaeI CATG 3 cut(s) 19, 467, 574
FaiI YATR 8 cut(s) 17, 71, 129, 305, 325, 465, 545, 572
FatI CATG 3 cut(s) 15, 463, 570
FbaI TGATCA 1 cut(s) 510
Fnu4HI GCNGC 3 cut(s) 37, 184, 297
FokI GGATG 2 cut(s) 32, 464
Fsp4HI GCNGC 3 cut(s) 37, 184, 297
FspI TGCGCA 1 cut(s) 173
GlaI GCGC 2 cut(s) 132, 173
GluI GCNGC 3 cut(s) 37, 184, 297
GsaI CCCAGC 2 cut(s) 214, 378
HgaI GACGC 1 cut(s) 404
HhaI GCGC 2 cut(s) 133, 174
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 3 cut(s) 19, 467, 574
Hin6I GCGC 2 cut(s) 131, 172
HinP1I GCGC 2 cut(s) 131, 172
HindIII AAGCTT 2 cut(s) 216, 483
HinfI GANTC 1 cut(s) 491
HphI GGTGA 1 cut(s) 278
Hpy188I TCNGA 3 cut(s) 195, 490, 496
Hpy188III TCNNGA 1 cut(s) 451
HpyAV CCTTC 3 cut(s) 409, 448, 529
HpyCH4III ACNGT 2 cut(s) 235, 279
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 7 cut(s) 26, 47, 97, 222, 422, 440, 501
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 180
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 541
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 3 cut(s) 19, 467, 574
HspAI GCGC 2 cut(s) 131, 172
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 4 cut(s) 201, 447, 510, 523
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 7 cut(s) 46, 150, 224, 299, 326, 388, 426
Lsp1109I GCAGC 2 cut(s) 48, 308
LweI GCATC 2 cut(s) 13, 427
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 2 cut(s) 149, 266
MalI GATC 4 cut(s) 203, 449, 512, 525
MboI GATC 4 cut(s) 201, 447, 510, 523
MboII GAAGA 2 cut(s) 19, 587
MfeI CAATTG 1 cut(s) 27
MluCI AATT 4 cut(s) 27, 98, 137, 557
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 5 cut(s) 117, 358, 361, 376, 547
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 3 cut(s) 81, 159, 564
MspA1I CMGCKG 1 cut(s) 299
MspR9I CCNGG 1 cut(s) 314
MunI CAATTG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 1 cut(s) 314
MwoI GCNNNNNNNGC 2 cut(s) 94, 180
NdeII GATC 4 cut(s) 201, 447, 510, 523
NlaIII CATG 3 cut(s) 19, 467, 574
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 266
NsbI TGCGCA 1 cut(s) 173
PctI GAATGC 1 cut(s) 503
PdmI GAANNNNTTC 1 cut(s) 11
PkrI GCNGC 3 cut(s) 38, 185, 298
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
Psp6I CCWGG 1 cut(s) 312
PspFI CCCAGC 2 cut(s) 210, 374
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 372
SaqAI TTAA 3 cut(s) 81, 159, 564
SatI GCNGC 3 cut(s) 37, 184, 297
Sau3AI GATC 4 cut(s) 201, 447, 510, 523
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 1 cut(s) 314
SfaNI GCATC 2 cut(s) 13, 427
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 4 cut(s) 27, 98, 137, 557
SsiI CCGC 2 cut(s) 184, 299
StyD4I CCNGG 1 cut(s) 312
TaaI ACNGT 2 cut(s) 235, 279
TaiI ACGT 1 cut(s) 544
TaqI TCGA 3 cut(s) 243, 435, 522
TasI AATT 4 cut(s) 27, 98, 137, 557
TauI GCSGC 1 cut(s) 186
Tru1I TTAA 3 cut(s) 81, 159, 564
Tru9I TTAA 3 cut(s) 81, 159, 564
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 2 cut(s) 36, 296
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 5 cut(s) 4, 58, 421, 429, 466
XapI RAATTY 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.