Rw7G003700

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016496
Physical Location & Seq
Forward (+)
3014024 .. 3014961
938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G003700.1

Sequence Viewer

Length: 567 bp
ATGGATAAAGCAATTGCCAGCAGCTCCATGGCACTTGTTGGAGTGGTTCTGTTTTTCTTAAGGATTATTAAGTGGCTCATTGTGCAATTCTTGCTACACTTGTCAAGTTTAAGGTATGCACTTCAATTCTTTGTAATTAATTGTGGTGTGAACAATGTTAACCTCGATGATCAGACCAAGATCACAACAGAGAAAGCTAGCACTTACAAAGTAGGTGTTGAAGAGAACACGAAAGACCTGGGTGACAAGAAACTGTGTTTAGGAGAATTAGCATCGGTTATGGAGAACCTGGGAAGTTTATGTGGTGATAATGGGTCTAAGGAGATGGCTGACTTGCTTGAGGAGGAACCCAGCTTGGAGGAAGTAAAAGAAGCCTTCCATTTGTTTGATGAAAATACCGATGGGTTCATCGATGCAGGGGAGATCAAGAAGGTTCTCCATGTATTAGGTTTCGTCCAAGCTTCGGAGTCAGAATGCAAAAGAATGATCAAGACCTTCGATCACAATGGAGATGGACGTATAGACTTTGAGGAATTTGTTAAGCTTATGGAGGATAGCTTCTGCTGA

Protein Analysis

188

Amino Acids

21.06

Weight (kDa)

4.65

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 120 - 183 4.9e-16 EF-hand domain pair
EF-hand_6 PF13405 121 - 150 9.8e-06 EF-hand domain
EF-hand_8 PF13833 135 - 185 2e-08 EF-hand domain pair
EF-hand_1 PF00036 158 - 184 1.1e-08 EF hand domain
EF-hand_5 PF13202 163 - 183 6.8e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47480
fragaria_vesca FvH4_5g14810
malus_domestica MD06G1194700.v1.1 MD14G1201700.v1.1
prunus_persica Prupe.5G194800_v2.0.a1
pyrus_communis pycom14g16710
rosa_chinensis RchiOBHm_Chr7g0181581
rosa_multiflora Rmu_sc0000988.1_g000019
rosa_roxburghii Rroxscaffold_3G00271880
rosa_samantha Rh7AG044600 Rh7BG043600 Rh7CG045400 Rh7DG044200
rosa_wichuraiana Rw7G003700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 533
AfiI CCNNNNNNNGG 1 cut(s) 463
AflII CTTAAG 1 cut(s) 58
AgsI TTSAA 2 cut(s) 125, 221
AjnI CCWGG 2 cut(s) 237, 288
AluBI AGCT 6 cut(s) 24, 197, 354, 461, 544, 558
AluI AGCT 6 cut(s) 24, 197, 354, 461, 544, 558
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 533
AseI ATTAAT 1 cut(s) 138
AsuHPI GGTGA 2 cut(s) 254, 317
AsuNHI GCTAGC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 33
BccI CCATC 3 cut(s) 319, 395, 506
BciT130I CCWGG 2 cut(s) 239, 290
BclI TGATCA 2 cut(s) 169, 486
BfaI CTAG 1 cut(s) 198
BfrI CTTAAG 1 cut(s) 58
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
Bme1390I CCNGG 2 cut(s) 239, 290
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 2 cut(s) 239, 290
BmsI GCATC 2 cut(s) 281, 403
BmtI GCTAGC 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 359
Bsa29I ATCGAT 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 27, 238, 289
Bsc4I CCNNNNNNNGG 1 cut(s) 463
BseBI CCWGG 2 cut(s) 239, 290
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 27, 238, 289
BseLI CCNNNNNNNGG 1 cut(s) 463
BseRI GAGGAG 1 cut(s) 356
BseXI GCAGC 1 cut(s) 33
BseYI CCCAGC 1 cut(s) 350
BshVI ATCGAT 1 cut(s) 411
BslI CCNNNNNNNGG 1 cut(s) 463
BsmI GAATGC 1 cut(s) 479
Bsp143I GATC 5 cut(s) 169, 180, 423, 486, 499
Bsp19I CCATGG 1 cut(s) 27
BspDI ATCGAT 1 cut(s) 411
BspLI GGNNCC 1 cut(s) 348
BspOI GCTAGC 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 58
BssECI CCNNGG 3 cut(s) 27, 238, 289
BssMI GATC 5 cut(s) 169, 180, 423, 486, 499
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 2 cut(s) 239, 290
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 58
BstAPI GCANNNNNTGC 1 cut(s) 91
BstC8I GCNNGC 2 cut(s) 19, 199
BstDEI CTNAG 1 cut(s) 318
BstDSI CCRYGG 1 cut(s) 27
BstKTI GATC 5 cut(s) 172, 183, 426, 489, 502
BstMBI GATC 5 cut(s) 169, 180, 423, 486, 499
BstMWI GCNNNNNNNGC 2 cut(s) 82, 91
BstNI CCWGG 2 cut(s) 239, 290
BstSCI CCNGG 2 cut(s) 237, 288
BstV1I GCAGC 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 411
BsuTUI ATCGAT 1 cut(s) 411
BtgI CCRYGG 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 19, 199
ClaI ATCGAT 1 cut(s) 411
CviAII CATG 2 cut(s) 28, 440
CviJI RGCY 9 cut(s) 24, 76, 197, 329, 354, 374, 461, 544, 558
CviKI_1 RGCY 9 cut(s) 24, 76, 197, 329, 354, 374, 461, 544, 558
DdeI CTNAG 1 cut(s) 318
DpnI GATC 5 cut(s) 171, 182, 425, 488, 501
DpnII GATC 5 cut(s) 169, 180, 423, 486, 499
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 27
EcoRII CCWGG 2 cut(s) 237, 288
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 31, 443
FaiI YATR 7 cut(s) 29, 117, 281, 301, 441, 521, 548
FatI CATG 2 cut(s) 27, 439
FbaI TGATCA 2 cut(s) 169, 486
Fnu4HI GCNGC 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 22
FspBI CTAG 1 cut(s) 198
GluI GCNGC 1 cut(s) 22
GsaI CCCAGC 1 cut(s) 354
Hin1II CATG 2 cut(s) 31, 443
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 2 cut(s) 459, 542
HinfI GANTC 1 cut(s) 467
HpaI GTTAAC 1 cut(s) 160
HphI GGTGA 2 cut(s) 254, 317
Hpy166II GTNNAC 2 cut(s) 151, 160
Hpy188I TCNGA 3 cut(s) 174, 466, 472
Hpy188III TCNNGA 2 cut(s) 427, 490
Hpy8I GTNNAC 2 cut(s) 151, 160
HpyAV CCTTC 3 cut(s) 385, 424, 505
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 4 cut(s) 85, 119, 416, 477
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 91
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 517
Hsp92II CATG 2 cut(s) 31, 443
Ksp22I TGATCA 2 cut(s) 169, 486
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 5 cut(s) 169, 180, 423, 486, 499
LmnI GCTCC 1 cut(s) 29
LpnPI CCDG 7 cut(s) 31, 224, 251, 275, 302, 364, 402
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 2 cut(s) 281, 403
MaeI CTAG 1 cut(s) 198
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 1 cut(s) 242
MalI GATC 5 cut(s) 171, 182, 425, 488, 501
MboI GATC 5 cut(s) 169, 180, 423, 486, 499
MboII GAAGA 1 cut(s) 233
MfeI CAATTG 1 cut(s) 12
MluCI AATT 7 cut(s) 12, 86, 125, 135, 139, 266, 533
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 6 cut(s) 173, 334, 337, 352, 523, 544
MseI TTAA 6 cut(s) 59, 69, 110, 138, 159, 540
MspCI CTTAAG 1 cut(s) 58
MspR9I CCNGG 2 cut(s) 239, 290
MunI CAATTG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 479
MvaI CCWGG 2 cut(s) 239, 290
MwoI GCNNNNNNNGC 2 cut(s) 82, 91
NcoI CCATGG 1 cut(s) 27
NdeII GATC 5 cut(s) 169, 180, 423, 486, 499
NheI GCTAGC 1 cut(s) 197
NlaIII CATG 2 cut(s) 31, 443
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 242
PctI GAATGC 1 cut(s) 479
PkrI GCNGC 1 cut(s) 23
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
PshBI ATTAAT 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 237, 288
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 2 cut(s) 237, 288
PspN4I GGNNCC 1 cut(s) 348
SaqAI TTAA 6 cut(s) 59, 69, 110, 138, 159, 540
SatI GCNGC 1 cut(s) 22
Sau3AI GATC 5 cut(s) 169, 180, 423, 486, 499
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 2 cut(s) 239, 290
SfaNI GCATC 2 cut(s) 281, 403
SmlI CTYRAG 2 cut(s) 58, 338
SmoI CTYRAG 2 cut(s) 58, 338
Sse9I AATT 7 cut(s) 12, 86, 125, 135, 139, 266, 533
SspMI CTAG 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 237, 288
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 1 cut(s) 520
TaqI TCGA 3 cut(s) 165, 411, 498
TasI AATT 7 cut(s) 12, 86, 125, 135, 139, 266, 533
Tru1I TTAA 6 cut(s) 59, 69, 110, 138, 159, 540
Tru9I TTAA 6 cut(s) 59, 69, 110, 138, 159, 540
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 242
TspDTI ATGAA 2 cut(s) 397, 405
Vha464I CTTAAG 1 cut(s) 58
VspI ATTAAT 1 cut(s) 138
XapI RAATTY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.