MD14G1201700.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016496
Physical Location & Seq
Forward (+)
29069483 .. 29070124
642 bp
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UTR
Exon/CDS
Intron
MD14G1201700.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGAGAAGGCGATGAGCGATGGGTTCGGATGCAGCCCCGATTCGCTTCTTGCACTTTTCTTAGTGATCCTAATCATTCTTAACTGGCTCCTTGTACTGCACTTCAACTCGAATTTACGCTACTTGTTTTTATGCTTCCTTTGCGATGTTATGCAGATTAAGCCCGTGATCCAAAGAAGTTGCGCTGACGAGAACGTATGTGATCACGAACCATCAGAAGGAGTTGAAACCAACTCTAAAACTACTAGTCATGACCAAGTCCCTTCAGTTCAGGCTGCGGAGATTAGTTACATTCACGAAAATGTTGCAGGTGGCGATAAGAATCTGTGTGCAGGAGAACTAGGACTAGTGATGGAGAAACTAGGAACATTAAGTGAGGGGAGACTCGGGTCGAAGGAGATTGCTGACTTGTTCGAGGAGGACCCGAGCTTGGAGGAAGTAAAAGAAGCTTTTCGCGTGTTTGATGAGAACGAGGACGGATTCATTGATGCAGGTGAGGTAATGAAGGTTCTCTCTGTATTGGGTTTCGTTGAAGTTTCGGAAGTGGAATGCAAACGGATGATCAAGGCATTTGATGTTGACGAAGATGGACGCATAGATTTCGATGAGTTTGTTAAACTCATGGAGAACAGCTTCTGCTGA

Protein Analysis

214

Amino Acids

23.76

Weight (kDa)

4.29

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 145 - 208 5.2e-15 EF-hand domain pair
EF-hand_1 PF00036 146 - 172 1e-05 EF hand domain
EF-hand_6 PF13405 146 - 175 3e-06 EF-hand domain
EF-hand_8 PF13833 158 - 210 2.4e-07 EF-hand domain pair
EF-hand_1 PF00036 183 - 209 7.5e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47480
fragaria_vesca FvH4_5g14810
malus_domestica MD06G1194700.v1.1 MD14G1201700.v1.1
prunus_persica Prupe.5G194800_v2.0.a1
pyrus_communis pycom14g16710
rosa_chinensis RchiOBHm_Chr7g0181581
rosa_multiflora Rmu_sc0000988.1_g000019
rosa_roxburghii Rroxscaffold_3G00271880
rosa_samantha Rh7AG044600 Rh7BG043600 Rh7CG045400 Rh7DG044200
rosa_wichuraiana Rw7G003700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 299, 482
Acc36I ACCTGC 2 cut(s) 299, 482
AccII CGCG 1 cut(s) 456
AciI CCGC 1 cut(s) 278
AclWI GGATC 2 cut(s) 61, 163
AcsI RAATTY 1 cut(s) 112
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 1 cut(s) 96
AfiI CCNNNNNNNGG 2 cut(s) 218, 430
AgsI TTSAA 3 cut(s) 106, 227, 533
AhlI ACTAGT 2 cut(s) 245, 346
AluBI AGCT 3 cut(s) 429, 449, 633
AluI AGCT 3 cut(s) 429, 449, 633
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 2 cut(s) 61, 163
AlwNI CAGNNNCTG 1 cut(s) 636
Ama87I CYCGRG 2 cut(s) 386, 424
ApeKI GCWGC 2 cut(s) 33, 275
ApoI RAATTY 1 cut(s) 112
Asp700I GAANNNNTTC 2 cut(s) 450, 632
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 421
AsuHPI GGTGA 1 cut(s) 506
AvaI CYCGRG 2 cut(s) 386, 424
AvaII GGWCC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 45, 262
BccI CCATC 4 cut(s) 14, 220, 346, 581
BcgI CGANNNNNNTGC 4 cut(s) 287, 321, 583, 617
BclI TGATCA 2 cut(s) 202, 561
BcoDI GTCTC 1 cut(s) 376
BcuI ACTAGT 2 cut(s) 245, 346
BfaI CTAG 4 cut(s) 246, 341, 347, 362
BfuAI ACCTGC 2 cut(s) 299, 482
BisI GCNGC 2 cut(s) 34, 276
BlsI GCNGC 2 cut(s) 35, 277
Bme18I GGWCC 1 cut(s) 421
BmeT110I CYCGRG 2 cut(s) 386, 424
BmgT120I GGNCC 1 cut(s) 421
BmiI GGNNCC 2 cut(s) 89, 423
BmsI GCATC 2 cut(s) 20, 478
BoxI GACNNNNGTC 1 cut(s) 388
BsaBI GATNNNNATC 2 cut(s) 71, 321
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 430
Bse1I ACTGG 1 cut(s) 89
Bse8I GATNNNNATC 2 cut(s) 71, 321
BseGI GGATG 2 cut(s) 35, 564
BseJI GATNNNNATC 2 cut(s) 71, 321
BseLI CCNNNNNNNGG 2 cut(s) 218, 430
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 2 cut(s) 45, 262
BsgI GTGCAG 2 cut(s) 83, 351
Bsh1236I CGCG 1 cut(s) 456
BsiHKCI CYCGRG 2 cut(s) 386, 424
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 2 cut(s) 218, 430
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 554
BsoBI CYCGRG 2 cut(s) 386, 424
Bsp143I GATC 4 cut(s) 66, 168, 202, 561
BspACI CCGC 1 cut(s) 278
BspFNI CGCG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 2 cut(s) 89, 423
BspMI ACCTGC 2 cut(s) 299, 482
BspPI GGATC 2 cut(s) 61, 163
BsrI ACTGG 1 cut(s) 89
BssMI GATC 4 cut(s) 66, 168, 202, 561
BstDEI CTNAG 1 cut(s) 61
BstF5I GGATG 2 cut(s) 35, 564
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 4 cut(s) 69, 171, 205, 564
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 4 cut(s) 66, 168, 202, 561
BstMWI GCNNNNNNNGC 2 cut(s) 141, 160
BstPAI GACNNNNGTC 1 cut(s) 388
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 45, 262
BtgZI GCGATG 3 cut(s) 26, 33, 159
BtsCI GGATG 2 cut(s) 35, 564
BveI ACCTGC 2 cut(s) 299, 482
CaiI CAGNNNCTG 1 cut(s) 636
CciI TCATGA 1 cut(s) 250
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 421
CseI GACGC 1 cut(s) 600
Csp6I GTAC 1 cut(s) 95
CviAII CATG 2 cut(s) 251, 622
CviJI RGCY 7 cut(s) 36, 88, 163, 275, 429, 449, 633
CviKI_1 RGCY 7 cut(s) 36, 88, 163, 275, 429, 449, 633
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 1 cut(s) 61
DpnI GATC 4 cut(s) 68, 170, 204, 563
DpnII GATC 4 cut(s) 66, 168, 202, 561
Eco47I GGWCC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 249
Eco88I CYCGRG 2 cut(s) 386, 424
EcoO109I RGGNCCY 1 cut(s) 421
FaeI CATG 2 cut(s) 254, 625
FaiI YATR 6 cut(s) 133, 152, 199, 252, 596, 623
FaqI GGGAC 1 cut(s) 245
FatI CATG 2 cut(s) 250, 621
FbaI TGATCA 2 cut(s) 202, 561
Fnu4HI GCNGC 2 cut(s) 34, 276
FokI GGATG 2 cut(s) 42, 571
Fsp4HI GCNGC 2 cut(s) 34, 276
FspBI CTAG 4 cut(s) 246, 341, 347, 362
GlaI GCGC 1 cut(s) 184
GluI GCNGC 2 cut(s) 34, 276
HgaI GACGC 1 cut(s) 600
HhaI GCGC 1 cut(s) 185
Hin1II CATG 2 cut(s) 254, 625
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 4 cut(s) 41, 322, 384, 480
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 3 cut(s) 29, 217, 541
Hpy188III TCNNGA 3 cut(s) 206, 251, 296
Hpy8I GTNNAC 1 cut(s) 580
HpyAV CCTTC 4 cut(s) 212, 273, 388, 499
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 8 cut(s) 33, 53, 100, 154, 308, 332, 491, 552
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 160
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 2 cut(s) 254, 625
HspAI GCGC 1 cut(s) 183
Ksp22I TGATCA 2 cut(s) 202, 561
Kzo9I GATC 4 cut(s) 66, 168, 202, 561
LmnI GCTCC 1 cut(s) 93
LpnPI CCDG 5 cut(s) 70, 257, 294, 318, 477
Lsp1109I GCAGC 2 cut(s) 45, 262
LweI GCATC 2 cut(s) 20, 478
MaeI CTAG 4 cut(s) 246, 341, 347, 362
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 287
MalI GATC 4 cut(s) 68, 170, 204, 563
MboI GATC 4 cut(s) 66, 168, 202, 561
MboII GAAGA 1 cut(s) 596
MluCI AATT 1 cut(s) 112
MlyI GAGTC 1 cut(s) 378
MnlI CCTC 6 cut(s) 370, 409, 412, 427, 466, 490
MroXI GAANNNNTTC 2 cut(s) 450, 632
MseI TTAA 4 cut(s) 81, 159, 371, 615
MslI CAYNNNNRTG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 554
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 141, 160
NdeII GATC 4 cut(s) 66, 168, 202, 561
NlaIII CATG 2 cut(s) 254, 625
NlaIV GGNNCC 2 cut(s) 89, 423
PagI TCATGA 1 cut(s) 250
PaqCI CACCTGC 2 cut(s) 299, 482
PctI GAATGC 1 cut(s) 554
PdmI GAANNNNTTC 2 cut(s) 450, 632
PfeI GAWTC 3 cut(s) 41, 322, 480
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 35, 277
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
PpuMI RGGWCCY 1 cut(s) 421
PshAI GACNNNNGTC 1 cut(s) 388
Psp5II RGGWCCY 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 89, 423
PspPI GGNCC 1 cut(s) 421
PspPPI RGGWCCY 1 cut(s) 421
PsrI GAACNNNNNNTAC 2 cut(s) 492, 524
PstNI CAGNNNCTG 1 cut(s) 636
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 4 cut(s) 81, 159, 371, 615
SatI GCNGC 2 cut(s) 34, 276
Sau3AI GATC 4 cut(s) 66, 168, 202, 561
Sau96I GGNCC 1 cut(s) 421
SchI GAGTC 1 cut(s) 378
SetI ASST 8 cut(s) 198, 313, 431, 451, 496, 501, 510, 635
SfaNI GCATC 2 cut(s) 20, 478
SinI GGWCC 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 300
SpeI ACTAGT 2 cut(s) 245, 346
Sse9I AATT 1 cut(s) 112
SsiI CCGC 1 cut(s) 278
SspMI CTAG 4 cut(s) 246, 341, 347, 362
TaiI ACGT 1 cut(s) 198
TaqI TCGA 4 cut(s) 110, 392, 414, 603
TasI AATT 1 cut(s) 112
TatI WGTACW 1 cut(s) 94
TfiI GAWTC 3 cut(s) 41, 322, 480
Tru1I TTAA 4 cut(s) 81, 159, 371, 615
Tru9I TTAA 4 cut(s) 81, 159, 371, 615
TseI GCWGC 2 cut(s) 33, 275
TspDTI ATGAA 2 cut(s) 472, 518
TspGWI ACGGA 2 cut(s) 492, 571
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 421
XapI RAATTY 1 cut(s) 112
XmnI GAANNNNTTC 2 cut(s) 450, 632
XspI CTAG 4 cut(s) 246, 341, 347, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.