AT3G49070

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006589
Physical Location & Seq
Reverse (-)
18192347 .. 18193867
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G49070.1

Sequence Viewer

Length: 1251 bp
ATGGGTATAAAAGTTTCATCAAAGATCAAAAGAATTCTCGCATCCACAGGTTATATTCCTATTTTCATTTTTAAATACCGCTATTATTTTTATTTTTTCTACTGCAAATTTGCAAGAAAATTTGTAACCAAGAAAATAATAATTTTGAAACTTTCCTCGATTGGTACAGCTTCAGGTAGTTCAAATCCCAAAGATGGTGCTGACGTGGATGTCCGAGAGGAATATGCAAACGCCTTTCGTACGGAATCATACAATCACTTTTGGACACGTGTCGTACAGCTAAGCCGCAAAAAGTCCGCAGTGTCTTCTTCTTCTTCTTCTCCACCAATCGAATCATCCTCCACGTCAGCTCGTCTCATGTCTTATCGCCTATTCGCGCACAATCTTCTAGACCCGGATCTGAATACCATTACTCGGATACTAGATGTATCCCGGGTCGGGCGACACACCCGAACTCTTCTCTCTGATTACTTCTTGGAGACGGCAAACGCTTTCTTGCTTTGCACGCAATTACTAAAAAACATTCATCATCTTCGTTCTAAATACGAGTCTTTGAAACCAAAATTTCATTCGGAAAATCATAATTCATTGGCTTTAATAGACCAATTCACGGAGATATCAAAATGGTTCGACCCGTTTATCTCGTCGGGTTCTAGGATCCAGCTAATCAGATCCGGCTGTTTATACTTGCTAAAACGGCTAGAGTCGAGACGAGACAAGACACGAGCCAAACTTAAGCTTATCAACGGACTAACTCATAGCTCAGGGCTTCTCGTTTTGGCTCTAACCACTACATTGATTGTAACCATAGCCTCTCACGCTTTTGCTTTGTTTCTAGCCGCTCCGACTCTTTTAGCCTCTCAATTTAAACCAGCCGGTTTAAGGAATAAGTTAACAAAAACAGCGGCTCGGCTCGACGTTGCTGCCAAAGGTACTTACATTTTAAGCCGTGATTTGGACACGATAAGCAGACTAGTGACACGAATTAACGATGAAGTGAACCATGTTCGAGCCATGGCTGAGTTTTGGGTTGGTAGAGGGTCGGGTCGGGTTCGAGGCAGTGAAGAAGTTGCTCGAGAGTTGAAGAGATGTGAAGAAAGCTTTAGTGAAGAACTTGATGAGCTTGAAGAACATATTTACTTGTGTTTCATGACTATTAATCGGGCAAGGAATCTTCTTGTTAAAGAGATTTTGGATTCGGATGATCCACCTAATTGTTCATTCGCTCCCAAATCCAAAAAAAAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

416

Amino Acids

47.27

Weight (kDa)

9.81

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 221 - 396 1.5e-07 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 842
AccII CGCG 1 cut(s) 377
AciI CCGC 5 cut(s) 79, 286, 297, 840, 905
AclWI GGATC 5 cut(s) 405, 652, 665, 666, 1199
AcsI RAATTY 4 cut(s) 33, 107, 119, 563
AcuI CTGAAG 1 cut(s) 156
AcvI CACGTG 1 cut(s) 269
AfaI GTAC 4 cut(s) 166, 241, 276, 934
AfiI CCNNNNNNNGG 6 cut(s) 194, 414, 438, 610, 882, 955
AflII CTTAAG 1 cut(s) 734
AflIII ACRYGT 2 cut(s) 266, 268
AgsI TTSAA 5 cut(s) 148, 183, 556, 1084, 1127
AhlI ACTAGT 1 cut(s) 973
AjiI CACGTC 2 cut(s) 205, 345
AjuI GAANNNNNNNTTGG 2 cut(s) 553, 585
AluBI AGCT 8 cut(s) 170, 280, 350, 664, 739, 762, 1101, 1123
AluI AGCT 8 cut(s) 170, 280, 350, 664, 739, 762, 1101, 1123
Alw26I GTCTC 4 cut(s) 359, 473, 703, 708
AlwI GGATC 5 cut(s) 405, 652, 665, 666, 1199
Ama87I CYCGRG 2 cut(s) 432, 1074
ApeKI GCWGC 1 cut(s) 923
ApoI RAATTY 4 cut(s) 33, 107, 119, 563
AseI ATTAAT 1 cut(s) 1158
AspLEI GCGC 1 cut(s) 379
AsuC2I CCSGG 3 cut(s) 395, 433, 434
AvaI CYCGRG 2 cut(s) 432, 1074
BamHI GGATCC 1 cut(s) 657
BauI CACGAG 1 cut(s) 723
BbrPI CACGTG 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 910
BccI CCATC 1 cut(s) 188
BceAI ACGGC 3 cut(s) 498, 713, 933
BcgI CGANNNNNNTGC 2 cut(s) 905, 939
BciVI GTATCC 2 cut(s) 411, 439
BcnI CCSGG 3 cut(s) 395, 433, 434
BcoDI GTCTC 4 cut(s) 359, 473, 703, 708
BcuI ACTAGT 1 cut(s) 973
BfaI CTAG 6 cut(s) 389, 422, 654, 701, 836, 974
BfrI CTTAAG 1 cut(s) 734
BfuI GTATCC 2 cut(s) 411, 439
BisI GCNGC 4 cut(s) 286, 840, 906, 924
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 4 cut(s) 287, 841, 907, 925
Bme1390I CCNGG 3 cut(s) 395, 433, 434
BmeT110I CYCGRG 2 cut(s) 432, 1074
BmgBI CACGTC 2 cut(s) 205, 345
BmiI GGNNCC 1 cut(s) 659
BmrFI CCNGG 3 cut(s) 395, 433, 434
BmsI GCATC 1 cut(s) 50
BoxI GACNNNNGTC 1 cut(s) 269
BpiI GAAGAC 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 763
Bpu1102I GCTNAGC 1 cut(s) 281
BpuMI CCSGG 3 cut(s) 395, 433, 434
BsaAI YACGTR 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 432, 1014
Bsc4I CCNNNNNNNGG 6 cut(s) 194, 414, 438, 610, 882, 955
Bse118I RCCGGY 1 cut(s) 875
BseDI CCNNGG 2 cut(s) 432, 1014
BseGI GGATG 4 cut(s) 41, 214, 335, 1207
BseLI CCNNNNNNNGG 6 cut(s) 194, 414, 438, 610, 882, 955
BseMII CTCAG 2 cut(s) 777, 1011
BseXI GCAGC 1 cut(s) 910
Bsh1236I CGCG 1 cut(s) 377
BsiHKCI CYCGRG 2 cut(s) 432, 1074
BsiSI CCGG 4 cut(s) 395, 433, 675, 876
BsiWI CGTACG 1 cut(s) 239
BslI CCNNNNNNNGG 6 cut(s) 194, 414, 438, 610, 882, 955
BsmAI GTCTC 4 cut(s) 359, 473, 703, 708
BsmBI CGTCTC 3 cut(s) 359, 473, 703
BsoBI CYCGRG 2 cut(s) 432, 1074
Bsp143I GATC 5 cut(s) 24, 397, 657, 671, 1204
Bsp1720I GCTNAGC 1 cut(s) 281
Bsp19I CCATGG 1 cut(s) 1014
BspACI CCGC 5 cut(s) 79, 286, 297, 840, 905
BspCNI CTCAG 2 cut(s) 776, 1012
BspFNI CGCG 1 cut(s) 377
BspHI TCATGA 1 cut(s) 1149
BspLI GGNNCC 1 cut(s) 659
BspPI GGATC 5 cut(s) 405, 652, 665, 666, 1199
BspTI CTTAAG 1 cut(s) 734
BsrBI CCGCTC 1 cut(s) 842
BsrFI RCCGGY 1 cut(s) 875
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 2 cut(s) 432, 1014
BssMI GATC 5 cut(s) 24, 397, 657, 671, 1204
BssSI CACGAG 1 cut(s) 723
BssT1I CCWWGG 1 cut(s) 1014
Bst2BI CACGAG 1 cut(s) 723
Bst6I CTCTTC 2 cut(s) 462, 1079
BstAFI CTTAAG 1 cut(s) 734
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 506
BstDEI CTNAG 3 cut(s) 281, 763, 1020
BstDSI CCRYGG 1 cut(s) 1014
BstF5I GGATG 4 cut(s) 41, 214, 335, 1207
BstFNI CGCG 1 cut(s) 377
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 5 cut(s) 27, 400, 660, 674, 1207
BstMAI GTCTC 4 cut(s) 359, 473, 703, 708
BstMBI GATC 5 cut(s) 24, 397, 657, 671, 1204
BstMWI GCNNNNNNNGC 3 cut(s) 505, 697, 818
BstPAI GACNNNNGTC 1 cut(s) 269
BstSCI CCNGG 3 cut(s) 393, 431, 432
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 1 cut(s) 910
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 3 cut(s) 397, 657, 671
BstYI RGATCY 3 cut(s) 397, 657, 671
BsuI GTATCC 2 cut(s) 411, 439
BtgI CCRYGG 1 cut(s) 1014
BtrI CACGTC 2 cut(s) 205, 345
BtsCI GGATG 4 cut(s) 41, 214, 335, 1207
BtsI GCAGTG 2 cut(s) 306, 1066
BtsIMutI CAGTG 2 cut(s) 306, 1066
Cac8I GCNNGC 1 cut(s) 506
CciI TCATGA 1 cut(s) 1149
CfoI GCGC 1 cut(s) 379
Cfr10I RCCGGY 1 cut(s) 875
Cfr9I CCCGGG 1 cut(s) 432
Csp6I GTAC 4 cut(s) 165, 240, 275, 933
CviAII CATG 4 cut(s) 358, 1004, 1015, 1150
CviQI GTAC 4 cut(s) 165, 240, 275, 933
DdeI CTNAG 3 cut(s) 281, 763, 1020
DpnI GATC 5 cut(s) 26, 399, 659, 673, 1206
DpnII GATC 5 cut(s) 24, 397, 657, 671, 1204
DraI TTTAAA 2 cut(s) 73, 868
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 462, 1079
EarI CTCTTC 2 cut(s) 462, 1079
Eco130I CCWWGG 1 cut(s) 1014
Eco32I GATATC 1 cut(s) 618
Eco57I CTGAAG 1 cut(s) 156
Eco72I CACGTG 1 cut(s) 269
Eco88I CYCGRG 2 cut(s) 432, 1074
EcoRI GAATTC 1 cut(s) 33
EcoRV GATATC 1 cut(s) 618
EcoT14I CCWWGG 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 1014
Esp3I CGTCTC 3 cut(s) 359, 473, 703
FaeI CATG 4 cut(s) 361, 1007, 1018, 1153
FatI CATG 4 cut(s) 357, 1003, 1014, 1149
Fnu4HI GCNGC 4 cut(s) 286, 840, 906, 924
FokI GGATG 4 cut(s) 28, 221, 322, 1214
Fsp4HI GCNGC 4 cut(s) 286, 840, 906, 924
FspBI CTAG 6 cut(s) 389, 422, 654, 701, 836, 974
GlaI GCGC 1 cut(s) 378
GluI GCNGC 4 cut(s) 286, 840, 906, 924
HapII CCGG 4 cut(s) 395, 433, 675, 876
HhaI GCGC 1 cut(s) 379
Hin1II CATG 4 cut(s) 361, 1007, 1018, 1153
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HincII GTYRAC 1 cut(s) 894
HindII GTYRAC 1 cut(s) 894
HindIII AAGCTT 2 cut(s) 737, 1099
HinfI GANTC 7 cut(s) 245, 332, 548, 704, 847, 1171, 1196
HpaI GTTAAC 1 cut(s) 894
HpaII CCGG 4 cut(s) 395, 433, 675, 876
Hpy166II GTNNAC 2 cut(s) 894, 1000
Hpy188I TCNGA 8 cut(s) 215, 402, 417, 466, 574, 671, 846, 1201
Hpy188III TCNNGA 4 cut(s) 389, 708, 1076, 1150
Hpy8I GTNNAC 2 cut(s) 894, 1000
Hpy99I CGWCG 2 cut(s) 649, 920
HpyCH4IV ACGT 4 cut(s) 204, 268, 344, 918
HpyCH4V TGCA 4 cut(s) 105, 113, 227, 504
HpyF10VI GCNNNNNNNGC 3 cut(s) 505, 697, 818
HpyF3I CTNAG 3 cut(s) 281, 763, 1020
HpySE526I ACGT 4 cut(s) 204, 268, 344, 918
Hsp92II CATG 4 cut(s) 361, 1007, 1018, 1153
HspAI GCGC 1 cut(s) 377
KspAI GTTAAC 1 cut(s) 894
Kzo9I GATC 5 cut(s) 24, 397, 657, 671, 1204
LmnI GCTCC 2 cut(s) 847, 1231
LpnPI CCDG 9 cut(s) 33, 159, 408, 446, 674, 688, 750, 885, 889
Lsp1109I GCAGC 1 cut(s) 910
LweI GCATC 1 cut(s) 50
MaeI CTAG 6 cut(s) 389, 422, 654, 701, 836, 974
MaeII ACGT 4 cut(s) 204, 268, 344, 918
MaeIII GTNAC 3 cut(s) 124, 802, 976
MalI GATC 5 cut(s) 26, 399, 659, 673, 1206
MbiI CCGCTC 1 cut(s) 842
MboI GATC 5 cut(s) 24, 397, 657, 671, 1204
MflI RGATCY 3 cut(s) 397, 657, 671
MlyI GAGTC 3 cut(s) 557, 713, 841
MmeI TCCRAC 1 cut(s) 869
MnlI CCTC 7 cut(s) 166, 211, 349, 823, 868, 1031, 1049
MspA1I CMGCKG 1 cut(s) 905
MspCI CTTAAG 1 cut(s) 734
MspI CCGG 4 cut(s) 395, 433, 675, 876
MspR9I CCNGG 3 cut(s) 395, 433, 434
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 505, 697, 818
NciI CCSGG 3 cut(s) 395, 433, 434
NcoI CCATGG 1 cut(s) 1014
NdeII GATC 5 cut(s) 24, 397, 657, 671, 1204
NlaIII CATG 4 cut(s) 361, 1007, 1018, 1153
NlaIV GGNNCC 1 cut(s) 659
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 1 cut(s) 976
PaeR7I CTCGAG 1 cut(s) 1074
PagI TCATGA 1 cut(s) 1149
PfeI GAWTC 4 cut(s) 245, 332, 1171, 1196
Pfl23II CGTACG 1 cut(s) 239
PkrI GCNGC 4 cut(s) 287, 841, 907, 925
PleI GAGTC 3 cut(s) 556, 712, 841
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 3 cut(s) 556, 712, 841
Ppu21I YACGTR 1 cut(s) 269
PshAI GACNNNNGTC 1 cut(s) 269
PshBI ATTAAT 1 cut(s) 1158
PspCI CACGTG 1 cut(s) 269
PspLI CGTACG 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 659
PsuI RGATCY 3 cut(s) 397, 657, 671
RsaI GTAC 4 cut(s) 166, 241, 276, 934
RsaNI GTAC 4 cut(s) 165, 240, 275, 933
SatI GCNGC 4 cut(s) 286, 840, 906, 924
Sau3AI GATC 5 cut(s) 24, 397, 657, 671, 1204
SchI GAGTC 3 cut(s) 557, 713, 841
ScrFI CCNGG 3 cut(s) 395, 433, 434
SfaNI GCATC 1 cut(s) 50
Sfr274I CTCGAG 1 cut(s) 1074
SlaI CTCGAG 1 cut(s) 1074
SmaI CCCGGG 1 cut(s) 434
SmlI CTYRAG 2 cut(s) 734, 1074
SmoI CTYRAG 2 cut(s) 734, 1074
SpeI ACTAGT 1 cut(s) 973
SsiI CCGC 5 cut(s) 79, 286, 297, 840, 905
SspMI CTAG 6 cut(s) 389, 422, 654, 701, 836, 974
StyD4I CCNGG 3 cut(s) 393, 431, 432
StyI CCWWGG 1 cut(s) 1014
TaiI ACGT 4 cut(s) 207, 271, 347, 921
TaqI TCGA 8 cut(s) 158, 330, 630, 707, 915, 1009, 1054, 1075
TauI GCSGC 3 cut(s) 288, 842, 908
TfiI GAWTC 4 cut(s) 245, 332, 1171, 1196
TscAI CASTG 2 cut(s) 306, 1066
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 1 cut(s) 923
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 8 cut(s) 6, 55, 515, 557, 576, 1008, 1138, 1209
TspGWI ACGGA 3 cut(s) 257, 626, 762
TspMI CCCGGG 1 cut(s) 432
TspRI CASTG 2 cut(s) 306, 1066
Vha464I CTTAAG 1 cut(s) 734
VspI ATTAAT 1 cut(s) 1158
XapI RAATTY 4 cut(s) 33, 107, 119, 563
XbaI TCTAGA 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 1074
XmaI CCCGGG 1 cut(s) 432
XspI CTAG 6 cut(s) 389, 422, 654, 701, 836, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.