pycom07g25160

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006589
Physical Location & Seq
Reverse (-)
25590597 .. 25591826
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25160.1

Sequence Viewer

Length: 1089 bp
ATGGGTAAAAGAATGAGTACATGCATCCGAAAATTTATTCCATTTACCGGATCTAGGCCCACTACATCAACTGCTTCAATGGAAGTGGATGTTACAGAAGAATATGCTAATGCTTTCCGCACCGAATCGTACCTCGACTTTTGGACACGTGTCGTTGCATTATCCAACAGTGAATCCACCAAACCGACGTGCAAGTCGATGGAGTCGACTACCGCAGCTCGGCTCCCATCCTATCGGCTCTTCGCGGAGCATCTATTAGATCCGGATCAACCCATGGTTACTCGGATCTTATCGTTGATTTGCAACCACCCGATCAATCACGTACTTTTATCTGACTATTTCACCCAAACCGCCGATGCTTCCATCCTATGTGGCCTCCTACTAAATGACATCAATCGTACACGTGTCAAATATCAGTCCATCAAATCCACCTTACAATCCTTAAATGACCATTCTACCCCTAAAACACTAACCCGTCTGACCAGATTCACCCCGATTTTATCTCTAATTCGCATCCAAGCCACTCAAGCCGGATGCTCTAACTTGCTGAAACGACTTGAGTCAACTCGTGACAAGGCTAGAGCCAAGCTCCGACTCGTAAAAAAGCTTCGAGCCGGCTCAACCATTTTCTTAGTCGCGTTAACGGCTTCGTTGAGCGTAATAGTTGTCACGCATGCTATTGCGCTAGTTGTGGCAATGCCGGCTTTTCTAGCGGCTTCGTTTGACTTGGCTTCACCGAAAAGGCTGATTAAGATGACATCTCAGCTCGACGCTGCCACGAAAGGAACTTACATACTAAACAGGGATTTGGAGACCGTGAGCCGACTCGTGGCTCGGCTAAACGATGAGCTTGAGCACATGCGTCGCATGGTCAAGTTTTGGGTGGAGCGTGGGGAGGAGGATTGGAGCCAAGCCGGAGGAGAACTGGCGCGCCAGCTGAAGAAAAATGACTGTAGCTTTAGGCAGCAGCTTGATGAGCTAGAGGAGCATTTGTATTTGTGTTTCATGACCATTAACAGGGCTAGAAATCTTGTTGTGAAGGAGATTCTGGATCCGGGTCAAATCATTCTGACCCATGATACATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

363

Amino Acids

40.91

Weight (kDa)

9.38

Isoelectric Point (pI)

33.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 69 - 349 2.4e-14 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 193
AccI GTMKAC 1 cut(s) 206
AccII CGCG 3 cut(s) 245, 638, 931
AccIII TCCGGA 1 cut(s) 262
AciI CCGC 5 cut(s) 118, 213, 245, 351, 713
AclWI GGATC 6 cut(s) 58, 254, 273, 293, 1046, 1059
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 959
AcvI CACGTG 2 cut(s) 149, 404
AfaI GTAC 4 cut(s) 19, 131, 324, 400
AfiI CCNNNNNNNGG 5 cut(s) 47, 54, 219, 829, 1017
AflIII ACRYGT 4 cut(s) 146, 148, 401, 403
AgsI TTSAA 1 cut(s) 78
AjiI CACGTC 1 cut(s) 189
AluBI AGCT 9 cut(s) 218, 589, 607, 766, 850, 937, 957, 970, 979
AluI AGCT 9 cut(s) 218, 589, 607, 766, 850, 937, 957, 970, 979
Alw21I GWGCWC 1 cut(s) 858
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 6 cut(s) 58, 254, 273, 293, 1046, 1059
Aor13HI TCCGGA 1 cut(s) 262
AoxI GGCC 2 cut(s) 56, 373
ApeKI GCWGC 4 cut(s) 215, 773, 964, 967
ApoI RAATTY 1 cut(s) 32
AscI GGCGCGCC 1 cut(s) 929
AspLEI GCGC 3 cut(s) 685, 931, 933
AspS9I GGNCC 1 cut(s) 57
AsuC2I CCSGG 1 cut(s) 1056
AsuHPI GGTGA 3 cut(s) 334, 481, 726
BamHI GGATCC 1 cut(s) 1051
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
BauI CACGAG 2 cut(s) 567, 827
BbrPI CACGTG 2 cut(s) 149, 404
Bbv12I GWGCWC 1 cut(s) 858
BbvI GCAGC 4 cut(s) 227, 760, 976, 979
BccI CCATC 4 cut(s) 193, 235, 371, 428
BceAI ACGGC 1 cut(s) 660
BcgI CGANNNNNNTGC 2 cut(s) 845, 879
BcnI CCSGG 1 cut(s) 1056
BcoDI GTCTC 1 cut(s) 806
BfaI CTAG 6 cut(s) 54, 579, 686, 710, 980, 1023
BfmI CTRYAG 1 cut(s) 952
BisI GCNGC 5 cut(s) 216, 714, 774, 965, 968
BlsI GCNGC 5 cut(s) 217, 715, 775, 966, 969
Bme1390I CCNGG 1 cut(s) 1056
BmgBI CACGTC 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 3 cut(s) 224, 908, 1053
BmrFI CCNGG 1 cut(s) 1056
BmsI GCATC 5 cut(s) 33, 259, 346, 522, 524
BoxI GACNNNNGTC 2 cut(s) 149, 559
BplI GAGNNNNNCTC 2 cut(s) 573, 605
BpuEI CTTGAG 3 cut(s) 510, 578, 872
BpuMI CCSGG 1 cut(s) 1056
BsaAI YACGTR 3 cut(s) 149, 322, 404
BsaBI GATNNNNATC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 806
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 2 cut(s) 47, 262
BsaXI ACNNNNNCTCC 2 cut(s) 977, 1007
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 54, 219, 829, 1017
Bse118I RCCGGY 2 cut(s) 614, 700
Bse1I ACTGG 1 cut(s) 930
Bse3DI GCAATG 1 cut(s) 702
Bse8I GATNNNNATC 1 cut(s) 264
BseAI TCCGGA 1 cut(s) 262
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 6 cut(s) 24, 94, 227, 363, 513, 539
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 5 cut(s) 47, 54, 219, 829, 1017
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 1 cut(s) 776
BseNI ACTGG 1 cut(s) 930
BsePI GCGCGC 1 cut(s) 929
BseRI GAGGAG 3 cut(s) 911, 933, 998
BseXI GCAGC 4 cut(s) 227, 760, 976, 979
Bsh1236I CGCG 3 cut(s) 245, 638, 931
BshFI GGCC 2 cut(s) 58, 375
BsiHKAI GWGCWC 1 cut(s) 858
BsiSI CCGG 7 cut(s) 48, 263, 531, 615, 701, 915, 1055
BslI CCNNNNNNNGG 5 cut(s) 47, 54, 219, 829, 1017
BsmAI GTCTC 1 cut(s) 806
BsnI GGCC 2 cut(s) 58, 375
Bso31I GGTCTC 1 cut(s) 806
Bsp1286I GDGCHC 1 cut(s) 858
Bsp13I TCCGGA 1 cut(s) 262
Bsp143I GATC 6 cut(s) 50, 259, 265, 285, 312, 1051
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 5 cut(s) 118, 213, 245, 351, 713
BspANI GGCC 2 cut(s) 58, 375
BspCNI CTCAG 1 cut(s) 775
BspEI TCCGGA 1 cut(s) 262
BspFNI CGCG 3 cut(s) 245, 638, 931
BspHI TCATGA 1 cut(s) 1005
BspLI GGNNCC 3 cut(s) 224, 908, 1053
BspPI GGATC 6 cut(s) 58, 254, 273, 293, 1046, 1059
BspQI GCTCTTC 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 806
BsrDI GCAATG 1 cut(s) 702
BsrFI RCCGGY 2 cut(s) 614, 700
BsrI ACTGG 1 cut(s) 930
BssAI RCCGGY 2 cut(s) 614, 700
BssECI CCNNGG 1 cut(s) 273
BssHII GCGCGC 1 cut(s) 929
BssMI GATC 6 cut(s) 50, 259, 265, 285, 312, 1051
BssSI CACGAG 2 cut(s) 567, 827
BssT1I CCWWGG 1 cut(s) 273
Bst2BI CACGAG 2 cut(s) 567, 827
Bst4CI ACNGT 3 cut(s) 170, 817, 953
Bst6I CTCTTC 1 cut(s) 245
BstBAI YACGTR 3 cut(s) 149, 322, 404
BstC8I GCNNGC 5 cut(s) 616, 675, 702, 931, 935
BstDEI CTNAG 2 cut(s) 631, 762
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 6 cut(s) 24, 94, 227, 363, 513, 539
BstFNI CGCG 3 cut(s) 245, 638, 931
BstHHI GCGC 3 cut(s) 685, 931, 933
BstKTI GATC 6 cut(s) 53, 262, 268, 288, 315, 1054
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 6 cut(s) 50, 259, 265, 285, 312, 1051
BstMWI GCNNNNNNNGC 6 cut(s) 527, 644, 701, 710, 976, 985
BstNSI RCATGY 3 cut(s) 24, 677, 862
BstPAI GACNNNNGTC 2 cut(s) 149, 559
BstSCI CCNGG 1 cut(s) 1054
BstSFI CTRYAG 1 cut(s) 952
BstUI CGCG 3 cut(s) 245, 638, 931
BstV1I GCAGC 4 cut(s) 227, 760, 976, 979
BstX2I RGATCY 4 cut(s) 50, 259, 285, 1051
BstYI RGATCY 4 cut(s) 50, 259, 285, 1051
BsuRI GGCC 2 cut(s) 58, 375
BtgI CCRYGG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 189
BtsCI GGATG 6 cut(s) 24, 94, 227, 363, 513, 539
BtsIMutI CAGTG 1 cut(s) 175
Cac8I GCNNGC 5 cut(s) 616, 675, 702, 931, 935
CciI TCATGA 1 cut(s) 1005
CfoI GCGC 3 cut(s) 685, 931, 933
Cfr10I RCCGGY 2 cut(s) 614, 700
Cfr13I GGNCC 1 cut(s) 57
CseI GACGC 2 cut(s) 779, 851
Csp6I GTAC 4 cut(s) 18, 130, 323, 399
CspCI CAANNNNNGTGG 2 cut(s) 66, 101
CviAII CATG 7 cut(s) 21, 274, 674, 859, 868, 1006, 1076
CviQI GTAC 4 cut(s) 18, 130, 323, 399
DdeI CTNAG 2 cut(s) 631, 762
DpnI GATC 6 cut(s) 52, 261, 267, 287, 314, 1053
DpnII GATC 6 cut(s) 50, 259, 265, 285, 312, 1051
DrdI GACNNNNNNGTC 1 cut(s) 193
DseDI GACNNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 806
Eco57I CTGAAG 1 cut(s) 959
Eco72I CACGTG 2 cut(s) 149, 404
EcoT14I CCWWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 7 cut(s) 24, 277, 677, 862, 871, 1009, 1079
FatI CATG 7 cut(s) 20, 273, 673, 858, 867, 1005, 1075
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 5 cut(s) 216, 714, 774, 965, 968
FokI GGATG 6 cut(s) 11, 101, 214, 350, 500, 546
Fsp4HI GCNGC 5 cut(s) 216, 714, 774, 965, 968
FspBI CTAG 6 cut(s) 54, 579, 686, 710, 980, 1023
GlaI GCGC 3 cut(s) 684, 930, 932
GluI GCNGC 5 cut(s) 216, 714, 774, 965, 968
HaeIII GGCC 2 cut(s) 58, 375
HapII CCGG 7 cut(s) 48, 263, 531, 615, 701, 915, 1055
HgaI GACGC 2 cut(s) 779, 851
HhaI GCGC 3 cut(s) 685, 931, 933
Hin1II CATG 7 cut(s) 24, 277, 677, 862, 871, 1009, 1079
Hin6I GCGC 3 cut(s) 683, 929, 931
HinP1I GCGC 3 cut(s) 683, 929, 931
HincII GTYRAC 3 cut(s) 207, 564, 642
HindII GTYRAC 3 cut(s) 207, 564, 642
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 8 cut(s) 125, 173, 203, 486, 560, 594, 825, 1045
HpaI GTTAAC 1 cut(s) 642
HpaII CCGG 7 cut(s) 48, 263, 531, 615, 701, 915, 1055
HphI GGTGA 3 cut(s) 334, 481, 726
Hpy166II GTNNAC 4 cut(s) 207, 401, 564, 642
Hpy188I TCNGA 6 cut(s) 29, 285, 334, 480, 593, 1071
Hpy188III TCNNGA 4 cut(s) 263, 569, 1006, 1049
Hpy8I GTNNAC 4 cut(s) 207, 401, 564, 642
Hpy99I CGWCG 3 cut(s) 190, 773, 867
HpyAV CCTTC 1 cut(s) 1033
HpyCH4III ACNGT 3 cut(s) 170, 817, 953
HpyCH4IV ACGT 4 cut(s) 148, 188, 321, 403
HpyCH4V TGCA 4 cut(s) 24, 158, 192, 303
HpyF10VI GCNNNNNNNGC 6 cut(s) 527, 644, 701, 710, 976, 985
HpyF3I CTNAG 2 cut(s) 631, 762
HpySE526I ACGT 4 cut(s) 148, 188, 321, 403
Hsp92II CATG 7 cut(s) 24, 277, 677, 862, 871, 1009, 1079
HspAI GCGC 3 cut(s) 683, 929, 931
Kpn2I TCCGGA 1 cut(s) 262
KroI GCCGGC 2 cut(s) 614, 700
KroNI GCCGGC 2 cut(s) 616, 702
KspAI GTTAAC 1 cut(s) 642
Kzo9I GATC 6 cut(s) 50, 259, 265, 285, 312, 1051
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 6 cut(s) 228, 247, 594, 886, 906, 985
Lsp1109I GCAGC 4 cut(s) 227, 760, 976, 979
LweI GCATC 5 cut(s) 33, 259, 346, 522, 524
MaeI CTAG 6 cut(s) 54, 579, 686, 710, 980, 1023
MaeII ACGT 4 cut(s) 148, 188, 321, 403
MaeIII GTNAC 4 cut(s) 91, 277, 569, 667
MalI GATC 6 cut(s) 52, 261, 267, 287, 314, 1053
MboI GATC 6 cut(s) 50, 259, 265, 285, 312, 1051
MboII GAAGA 3 cut(s) 110, 232, 952
MflI RGATCY 4 cut(s) 50, 259, 285, 1051
MhlI GDGCHC 1 cut(s) 858
MluCI AATT 2 cut(s) 32, 507
MlyI GAGTC 4 cut(s) 212, 569, 588, 819
MmeI TCCRAC 2 cut(s) 189, 616
MnlI CCTC 6 cut(s) 143, 386, 889, 892, 911, 976
Mph1103I ATGCAT 1 cut(s) 26
MroI TCCGGA 1 cut(s) 262
MroNI GCCGGC 2 cut(s) 614, 700
MseI TTAA 4 cut(s) 443, 641, 750, 1014
MspA1I CMGCKG 1 cut(s) 937
MspI CCGG 7 cut(s) 48, 263, 531, 615, 701, 915, 1055
MspR9I CCNGG 1 cut(s) 1056
MvnI CGCG 3 cut(s) 245, 638, 931
MwoI GCNNNNNNNGC 6 cut(s) 527, 644, 701, 710, 976, 985
NaeI GCCGGC 2 cut(s) 616, 702
NciI CCSGG 1 cut(s) 1056
NcoI CCATGG 1 cut(s) 273
NdeII GATC 6 cut(s) 50, 259, 265, 285, 312, 1051
NgoMIV GCCGGC 2 cut(s) 614, 700
NlaIII CATG 7 cut(s) 24, 277, 677, 862, 871, 1009, 1079
NlaIV GGNNCC 3 cut(s) 224, 908, 1053
NmeAIII GCCGAG 2 cut(s) 199, 814
NmuCI GTSAC 2 cut(s) 569, 667
NsiI ATGCAT 1 cut(s) 26
NspI RCATGY 3 cut(s) 24, 677, 862
PaeI GCATGC 1 cut(s) 677
PagI TCATGA 1 cut(s) 1005
PalAI GGCGCGCC 1 cut(s) 929
PauI GCGCGC 1 cut(s) 929
PciSI GCTCTTC 1 cut(s) 245
PcsI WCGNNNNNNNCGW 2 cut(s) 194, 203
PdiI GCCGGC 2 cut(s) 616, 702
PfeI GAWTC 4 cut(s) 125, 173, 486, 1045
PkrI GCNGC 5 cut(s) 217, 715, 775, 966, 969
PleI GAGTC 4 cut(s) 211, 568, 588, 819
PmaCI CACGTG 2 cut(s) 149, 404
PmlI CACGTG 2 cut(s) 149, 404
PpsI GAGTC 4 cut(s) 211, 568, 588, 819
Ppu21I YACGTR 3 cut(s) 149, 322, 404
PshAI GACNNNNGTC 2 cut(s) 149, 559
PspCI CACGTG 2 cut(s) 149, 404
PspN4I GGNNCC 3 cut(s) 224, 908, 1053
PspPI GGNCC 1 cut(s) 57
PsuI RGATCY 4 cut(s) 50, 259, 285, 1051
PteI GCGCGC 1 cut(s) 929
PvuII CAGCTG 1 cut(s) 937
RsaI GTAC 4 cut(s) 19, 131, 324, 400
RsaNI GTAC 4 cut(s) 18, 130, 323, 399
SalI GTCGAC 1 cut(s) 205
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 4 cut(s) 443, 641, 750, 1014
SatI GCNGC 5 cut(s) 216, 714, 774, 965, 968
Sau3AI GATC 6 cut(s) 50, 259, 265, 285, 312, 1051
Sau96I GGNCC 1 cut(s) 57
SchI GAGTC 4 cut(s) 212, 569, 588, 819
ScrFI CCNGG 1 cut(s) 1056
SduI GDGCHC 1 cut(s) 858
SfaNI GCATC 5 cut(s) 33, 259, 346, 522, 524
SfcI CTRYAG 1 cut(s) 952
SgsI GGCGCGCC 1 cut(s) 929
SmlI CTYRAG 3 cut(s) 525, 557, 851
SmoI CTYRAG 3 cut(s) 525, 557, 851
SphI GCATGC 1 cut(s) 677
Sse9I AATT 2 cut(s) 32, 507
SsiI CCGC 5 cut(s) 118, 213, 245, 351, 713
SspMI CTAG 6 cut(s) 54, 579, 686, 710, 980, 1023
StyD4I CCNGG 1 cut(s) 1054
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 3 cut(s) 170, 817, 953
TaiI ACGT 4 cut(s) 151, 191, 324, 406
TaqI TCGA 5 cut(s) 135, 197, 206, 610, 768
TasI AATT 2 cut(s) 32, 507
TatI WGTACW 1 cut(s) 17
TauI GCSGC 1 cut(s) 716
TfiI GAWTC 4 cut(s) 125, 173, 486, 1045
Tru1I TTAA 4 cut(s) 443, 641, 750, 1014
Tru9I TTAA 4 cut(s) 443, 641, 750, 1014
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 2 cut(s) 569, 667
TseI GCWGC 4 cut(s) 215, 773, 964, 967
Tsp45I GTSAC 2 cut(s) 569, 667
TspDTI ATGAA 1 cut(s) 994
TspRI CASTG 1 cut(s) 175
XapI RAATTY 1 cut(s) 32
XceI RCATGY 3 cut(s) 24, 677, 862
XmiI GTMKAC 1 cut(s) 206
XspI CTAG 6 cut(s) 54, 579, 686, 710, 980, 1023
Zsp2I ATGCAT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.