Rh1AG425600

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006589
Physical Location & Seq
Forward (+)
65445552 .. 65446568
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG425600.1

Sequence Viewer

Length: 1017 bp
ATGGGAGTGGATGTAACAGAAGAATACGCTAATGCCTTCCGCACCGAATCCTACCTCGACTTTTGGACACGTGTCGTCACGCTATCCAATGCTGATTCCACCGGCTCCAAGCTTATTCAAATGGAGTCGAGAACTGCGGCTCGGCTCCCATCCTATCGGCTCTTCGTGGAGCATCTTTTGGATCCGGATCAAACCATGATTACTCGGATCTTGGCACTGACCCGAAACCACCCAAAGAACCATGCACTTCTATCCGACTATTTTACCCAAACCGCAGATGCTTCCGCCCTGTGTGGTCTCCTACTAAAAGATATCGACCGTACACGTGTTAAATACCGTTCCATCAGAACTACACTTCAGTCTCTAAATAAATGTCTAGCTCCTACGACACTAAACCAACTAACCAAATTCTCTATTGTTTTATCTCCCAAACGAATTCAAAGTATTCAAGTTAGATGCTCTGATTTGTTGAAACGGCTCGAGTTAACTCGCAACAAGACTCGAGCTAAGCTAAGTCTCACGGGCAAATTCCAAACCGGATCGGCTATCATCTTGGTGGCAATAACGACTACACTAAGTGTAATTGTTGTCACGCATGCACTTGCACTGGTTGTAGCTGCACCAACTCTTATAGCAACCTCACTCGACCTGGCTTCGTCCAGGAGACTAGCTAGGATGGCGGCTCAGCTCGATGCGGCTACCAAGGGAGCTTACATAGTAAACAGGGATTTGGAGACGGTGAGCCGGCTCGTGGCTCGGCTAAACGATGAGCTTGAGCACATGCGCGGAATGGTTCAGTTTTGGGTGGAGCGCGGCGAGGAGGACTGGCTCCAAGCCGGTGGCGAAGTGGCACGCCAGCTGAAGAAAAATGATTGTAGCTTTAGACAGCAGCTAGATGAGCTGGAGGAGCATTTGTATCTGTGTTTCATGACTATAAACCGGGCTAGAAATCTTGTAGTGAAGGAGATTCTAGATCCCGGTCAGACCACCAATTCACATACCACATTAGCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

338

Amino Acids

38.02

Weight (kDa)

9.3

Isoelectric Point (pI)

29.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 47 - 323 6.5e-13 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 786, 813
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 8 cut(s) 40, 137, 273, 285, 680, 695, 786, 813
AclWI GGATC 6 cut(s) 176, 189, 195, 215, 547, 968
AcsI RAATTY 3 cut(s) 407, 435, 527
AcuI CTGAAG 2 cut(s) 341, 881
AcvI CACGTG 2 cut(s) 71, 326
AdeI CACNNNGTG 1 cut(s) 578
AfaI GTAC 1 cut(s) 322
AfiI CCNNNNNNNGG 1 cut(s) 751
AflIII ACRYGT 4 cut(s) 68, 70, 323, 325
AgsI TTSAA 4 cut(s) 119, 440, 449, 472
AjnI CCWGG 2 cut(s) 648, 659
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 5 cut(s) 302, 366, 521, 658, 728
AlwI GGATC 6 cut(s) 176, 189, 195, 215, 547, 968
Ama87I CYCGRG 2 cut(s) 479, 501
Aor13HI TCCGGA 1 cut(s) 184
ApeKI GCWGC 2 cut(s) 617, 889
ApoI RAATTY 3 cut(s) 407, 435, 527
AspLEI GCGC 2 cut(s) 786, 813
AsuC2I CCSGG 2 cut(s) 941, 978
AsuHPI GGTGA 1 cut(s) 751
AvaI CYCGRG 2 cut(s) 479, 501
BamHI GGATCC 1 cut(s) 181
BauI CACGAG 1 cut(s) 749
BbrPI CACGTG 2 cut(s) 71, 326
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 2 cut(s) 604, 901
BccI CCATC 3 cut(s) 157, 350, 670
BceAI ACGGC 1 cut(s) 491
BciT130I CCWGG 2 cut(s) 650, 661
BcnI CCSGG 2 cut(s) 941, 978
BcoDI GTCTC 5 cut(s) 302, 366, 521, 658, 728
BfaI CTAG 6 cut(s) 377, 668, 672, 893, 945, 971
BisI GCNGC 6 cut(s) 138, 618, 681, 696, 814, 890
BlpI GCTNAGC 2 cut(s) 507, 684
BlsI GCNGC 6 cut(s) 139, 619, 682, 697, 815, 891
Bme1390I CCNGG 4 cut(s) 650, 661, 941, 978
BmeT110I CYCGRG 2 cut(s) 479, 501
BmiI GGNNCC 4 cut(s) 106, 146, 183, 830
BmrFI CCNGG 4 cut(s) 650, 661, 941, 978
BmsI GCATC 4 cut(s) 181, 268, 446, 682
BoxI GACNNNNGTC 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 923
Bpu1102I GCTNAGC 2 cut(s) 507, 684
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 2 cut(s) 941, 978
BsaAI YACGTR 2 cut(s) 71, 326
BsaBI GATNNNNATC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 702
BsaWI WCCGGW 2 cut(s) 184, 536
BsaXI ACNNNNNCTCC 3 cut(s) 27, 899, 929
Bsc4I CCNNNNNNNGG 1 cut(s) 751
Bse118I RCCGGY 3 cut(s) 101, 744, 836
Bse1I ACTGG 2 cut(s) 612, 830
Bse3DI GCAATG 1 cut(s) 1017
Bse8I GATNNNNATC 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 184
BseBI CCWGG 2 cut(s) 650, 661
BseDI CCNNGG 1 cut(s) 702
BseGI GGATG 3 cut(s) 16, 149, 681
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 1 cut(s) 751
BseMI GCAATG 1 cut(s) 1017
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 2 cut(s) 612, 830
BseRI GAGGAG 2 cut(s) 833, 920
BseXI GCAGC 2 cut(s) 604, 901
BsgI GTGCAG 1 cut(s) 603
Bsh1236I CGCG 2 cut(s) 786, 813
Bsh1285I CGRYCG 1 cut(s) 319
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 780
BsiHKCI CYCGRG 2 cut(s) 479, 501
BsiSI CCGG 7 cut(s) 102, 185, 537, 745, 837, 940, 978
BslI CCNNNNNNNGG 1 cut(s) 751
BsmAI GTCTC 5 cut(s) 302, 366, 521, 658, 728
BsmBI CGTCTC 1 cut(s) 728
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 2 cut(s) 479, 501
Bsp1286I GDGCHC 1 cut(s) 780
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 5 cut(s) 181, 187, 207, 539, 973
Bsp1720I GCTNAGC 2 cut(s) 507, 684
BspACI CCGC 8 cut(s) 40, 137, 273, 285, 680, 695, 786, 813
BspCNI CTCAG 1 cut(s) 697
BspEI TCCGGA 1 cut(s) 184
BspFNI CGCG 2 cut(s) 786, 813
BspHI TCATGA 1 cut(s) 927
BspLI GGNNCC 4 cut(s) 106, 146, 183, 830
BspPI GGATC 6 cut(s) 176, 189, 195, 215, 547, 968
BspQI GCTCTTC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 1017
BsrFI RCCGGY 3 cut(s) 101, 744, 836
BsrI ACTGG 2 cut(s) 612, 830
BssAI RCCGGY 3 cut(s) 101, 744, 836
BssECI CCNNGG 1 cut(s) 702
BssMI GATC 5 cut(s) 181, 187, 207, 539, 973
BssSI CACGAG 1 cut(s) 749
BssT1I CCWWGG 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 749
Bst2UI CCWGG 2 cut(s) 650, 661
Bst4CI ACNGT 3 cut(s) 320, 338, 739
Bst6I CTCTTC 1 cut(s) 167
BstBAI YACGTR 2 cut(s) 71, 326
BstC8I GCNNGC 4 cut(s) 597, 746, 853, 857
BstDEI CTNAG 4 cut(s) 507, 512, 575, 684
BstF5I GGATG 3 cut(s) 16, 149, 681
BstFNI CGCG 2 cut(s) 786, 813
BstHHI GCGC 2 cut(s) 786, 813
BstKTI GATC 5 cut(s) 184, 190, 210, 542, 976
BstMAI GTCTC 5 cut(s) 302, 366, 521, 658, 728
BstMBI GATC 5 cut(s) 181, 187, 207, 539, 973
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 3 cut(s) 677, 898, 907
BstNI CCWGG 2 cut(s) 650, 661
BstNSI RCATGY 2 cut(s) 599, 784
BstPAI GACNNNNGTC 1 cut(s) 71
BstSCI CCNGG 4 cut(s) 648, 659, 939, 976
BstUI CGCG 2 cut(s) 786, 813
BstV1I GCAGC 2 cut(s) 604, 901
BstX2I RGATCY 3 cut(s) 181, 207, 973
BstXI CCANNNNNNTGG 1 cut(s) 839
BstYI RGATCY 3 cut(s) 181, 207, 973
BtsCI GGATG 3 cut(s) 16, 149, 681
BtsIMutI CAGTG 2 cut(s) 215, 605
Cac8I GCNNGC 4 cut(s) 597, 746, 853, 857
CciI TCATGA 1 cut(s) 927
CfoI GCGC 2 cut(s) 786, 813
Cfr10I RCCGGY 3 cut(s) 101, 744, 836
Csp6I GTAC 1 cut(s) 321
CviAII CATG 5 cut(s) 196, 242, 596, 781, 928
CviQI GTAC 1 cut(s) 321
DdeI CTNAG 4 cut(s) 507, 512, 575, 684
DpnI GATC 5 cut(s) 183, 189, 209, 541, 975
DpnII GATC 5 cut(s) 181, 187, 207, 539, 973
DraIII CACNNNGTG 1 cut(s) 578
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EciI GGCGGA 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 702
Eco31I GGTCTC 1 cut(s) 302
Eco32I GATATC 1 cut(s) 313
Eco57I CTGAAG 2 cut(s) 341, 881
Eco72I CACGTG 2 cut(s) 71, 326
Eco88I CYCGRG 2 cut(s) 479, 501
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 2 cut(s) 648, 659
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 702
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 5 cut(s) 199, 245, 599, 784, 931
FaiI YATR 9 cut(s) 197, 243, 597, 632, 716, 782, 929, 935, 999
FatI CATG 5 cut(s) 195, 241, 595, 780, 927
Fnu4HI GCNGC 6 cut(s) 138, 618, 681, 696, 814, 890
FokI GGATG 3 cut(s) 23, 136, 688
Fsp4HI GCNGC 6 cut(s) 138, 618, 681, 696, 814, 890
FspBI CTAG 6 cut(s) 377, 668, 672, 893, 945, 971
GlaI GCGC 2 cut(s) 785, 812
GluI GCNGC 6 cut(s) 138, 618, 681, 696, 814, 890
GsuI CTGGAG 1 cut(s) 923
HapII CCGG 7 cut(s) 102, 185, 537, 745, 837, 940, 978
HhaI GCGC 2 cut(s) 786, 813
Hin1II CATG 5 cut(s) 199, 245, 599, 784, 931
Hin6I GCGC 2 cut(s) 784, 811
HinP1I GCGC 2 cut(s) 784, 811
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 5 cut(s) 47, 95, 125, 499, 967
HpaI GTTAAC 1 cut(s) 486
HpaII CCGG 7 cut(s) 102, 185, 537, 745, 837, 940, 978
HphI GGTGA 1 cut(s) 751
Hpy166II GTNNAC 3 cut(s) 323, 486, 721
Hpy188I TCNGA 5 cut(s) 207, 256, 347, 463, 984
Hpy188III TCNNGA 4 cut(s) 129, 185, 928, 971
Hpy8I GTNNAC 3 cut(s) 323, 486, 721
HpyAV CCTTC 2 cut(s) 46, 955
HpyCH4III ACNGT 3 cut(s) 320, 338, 739
HpyCH4IV ACGT 2 cut(s) 70, 325
HpyCH4V TGCA 4 cut(s) 245, 599, 605, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 677, 898, 907
HpyF3I CTNAG 4 cut(s) 507, 512, 575, 684
HpySE526I ACGT 2 cut(s) 70, 325
Hsp92II CATG 5 cut(s) 199, 245, 599, 784, 931
HspAI GCGC 2 cut(s) 784, 811
Kpn2I TCCGGA 1 cut(s) 184
KroI GCCGGC 1 cut(s) 744
KroNI GCCGGC 1 cut(s) 746
KspAI GTTAAC 1 cut(s) 486
Kzo9I GATC 5 cut(s) 181, 187, 207, 539, 973
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 8 cut(s) 110, 150, 169, 385, 707, 808, 834, 907
Lsp1109I GCAGC 2 cut(s) 604, 901
LweI GCATC 4 cut(s) 181, 268, 446, 682
MaeI CTAG 6 cut(s) 377, 668, 672, 893, 945, 971
MaeII ACGT 2 cut(s) 70, 325
MaeIII GTNAC 3 cut(s) 13, 76, 589
MalI GATC 5 cut(s) 183, 189, 209, 541, 975
MboI GATC 5 cut(s) 181, 187, 207, 539, 973
MboII GAAGA 3 cut(s) 32, 154, 874
MflI RGATCY 3 cut(s) 181, 207, 973
MhlI GDGCHC 1 cut(s) 780
MluCI AATT 5 cut(s) 407, 435, 527, 582, 991
MlyI GAGTC 2 cut(s) 134, 493
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 5 cut(s) 65, 649, 811, 814, 898
MroI TCCGGA 1 cut(s) 184
MroNI GCCGGC 1 cut(s) 744
MseI TTAA 2 cut(s) 330, 485
MslI CAYNNNNRTG 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 859
MspI CCGG 7 cut(s) 102, 185, 537, 745, 837, 940, 978
MspR9I CCNGG 4 cut(s) 650, 661, 941, 978
MvaI CCWGG 2 cut(s) 650, 661
MvnI CGCG 2 cut(s) 786, 813
MwoI GCNNNNNNNGC 3 cut(s) 677, 898, 907
NaeI GCCGGC 1 cut(s) 746
NciI CCSGG 2 cut(s) 941, 978
NdeII GATC 5 cut(s) 181, 187, 207, 539, 973
NgoMIV GCCGGC 1 cut(s) 744
NlaIII CATG 5 cut(s) 199, 245, 599, 784, 931
NlaIV GGNNCC 4 cut(s) 106, 146, 183, 830
NmeAIII GCCGAG 2 cut(s) 121, 736
NmuCI GTSAC 2 cut(s) 76, 589
NspI RCATGY 2 cut(s) 599, 784
PaeI GCATGC 1 cut(s) 599
PaeR7I CTCGAG 2 cut(s) 479, 501
PagI TCATGA 1 cut(s) 927
PciSI GCTCTTC 1 cut(s) 167
PdiI GCCGGC 1 cut(s) 746
PfeI GAWTC 3 cut(s) 47, 95, 967
PfoI TCCNGGA 1 cut(s) 659
PkrI GCNGC 6 cut(s) 139, 619, 682, 697, 815, 891
PleI GAGTC 2 cut(s) 133, 493
PmaCI CACGTG 2 cut(s) 71, 326
PmlI CACGTG 2 cut(s) 71, 326
PpsI GAGTC 2 cut(s) 133, 493
Ppu21I YACGTR 2 cut(s) 71, 326
PshAI GACNNNNGTC 1 cut(s) 71
Psp6I CCWGG 2 cut(s) 648, 659
PspCI CACGTG 2 cut(s) 71, 326
PspGI CCWGG 2 cut(s) 648, 659
PspN4I GGNNCC 4 cut(s) 106, 146, 183, 830
PspXI VCTCGAGB 2 cut(s) 479, 501
PsuI RGATCY 3 cut(s) 181, 207, 973
PvuII CAGCTG 1 cut(s) 859
RsaI GTAC 1 cut(s) 322
RsaNI GTAC 1 cut(s) 321
RseI CAYNNNNRTG 1 cut(s) 554
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 2 cut(s) 330, 485
SatI GCNGC 6 cut(s) 138, 618, 681, 696, 814, 890
Sau3AI GATC 5 cut(s) 181, 187, 207, 539, 973
SchI GAGTC 2 cut(s) 134, 493
ScrFI CCNGG 4 cut(s) 650, 661, 941, 978
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 4 cut(s) 181, 268, 446, 682
Sfr274I CTCGAG 2 cut(s) 479, 501
SlaI CTCGAG 2 cut(s) 479, 501
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 3 cut(s) 479, 501, 773
SmoI CTYRAG 3 cut(s) 479, 501, 773
SphI GCATGC 1 cut(s) 599
Sse9I AATT 5 cut(s) 407, 435, 527, 582, 991
SsiI CCGC 8 cut(s) 40, 137, 273, 285, 680, 695, 786, 813
SspMI CTAG 6 cut(s) 377, 668, 672, 893, 945, 971
StyD4I CCNGG 4 cut(s) 648, 659, 939, 976
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 3 cut(s) 320, 338, 739
TaiI ACGT 2 cut(s) 73, 328
TaqI TCGA 7 cut(s) 57, 128, 315, 480, 502, 645, 690
TasI AATT 5 cut(s) 407, 435, 527, 582, 991
TauI GCSGC 4 cut(s) 140, 683, 698, 816
TfiI GAWTC 3 cut(s) 47, 95, 967
Tru1I TTAA 2 cut(s) 330, 485
Tru9I TTAA 2 cut(s) 330, 485
TscAI CASTG 2 cut(s) 222, 612
TseFI GTSAC 2 cut(s) 76, 589
TseI GCWGC 2 cut(s) 617, 889
Tsp45I GTSAC 2 cut(s) 76, 589
TspDTI ATGAA 1 cut(s) 916
TspRI CASTG 2 cut(s) 222, 612
XapI RAATTY 3 cut(s) 407, 435, 527
XbaI TCTAGA 1 cut(s) 970
XceI RCATGY 2 cut(s) 599, 784
XhoI CTCGAG 2 cut(s) 479, 501
XspI CTAG 6 cut(s) 377, 668, 672, 893, 945, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.