Rroxscaffold_4G00280900

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006589
Physical Location & Seq
Forward (+)
3346334 .. 3347278
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280900.1

Sequence Viewer

Length: 711 bp
ATGGGAGTGGATGTAACAGAAGAATACGCTAATGCCTTCCGCACCGAATCCTACCTCGACTTTTGGACACGTGTTGTCACGCTATCCAATGCTGATTCCACCGGCTCCAAGCTTATTCAAATGGAGTCGACAACTGCGGCTCGGCTCCCATCCTATCGGCTCTTCGTGGAGCATCTTTTGGATCCGGATCAAACCATGATTACTCGGATCTTGGCACTGACCCGAAGCCACCCAAAGAACCACGCACTTCTATCCGACTATTTTACCCAAACCGCAGATGCTTCCGCCCTGTGTGGTCTCCTACTAAAAGATATCGACCGTATACTAACGACTACACTAAGTGTAATTGTTGTCACGCATGCACTCGCACTAGTTGTAGCTGCACCAACTCTTATAGCAACCTCACTCGACTTGGCTTCGTCAAGGAGACTAGCTAGGATGGCGGCTCAGCTCGATGCGGCTACCAAGGGAGCTTACATAGTAAACAGGGATTTGGAGACGGTGAGCCGGCTTGTGGCTCGGCTAAACGATGAGCTTGAGCACATGCGCGGAATGGTTCAGTTTTGGGTGGAGCGCGGCGAGGAGGACTGGCTCCAAGCCGGCGGCGAAGTGGCACGCCAGCTGAAGAAAAATGATTGTAGCTTTAGACAGCAGCTAGATGAGCTGGAGGAGCATTTGTACCTGTGTTTCATGACTATAAACCGGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

236

Amino Acids

26.51

Weight (kDa)

5.39

Isoelectric Point (pI)

24.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 128, 322
AccII CGCG 2 cut(s) 549, 576
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 9 cut(s) 40, 137, 273, 285, 443, 458, 549, 576, 603
AclWI GGATC 4 cut(s) 176, 189, 195, 215
AcuI CTGAAG 1 cut(s) 644
AcvI CACGTG 1 cut(s) 71
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 1 cut(s) 680
AfiI CCNNNNNNNGG 1 cut(s) 514
AflIII ACRYGT 2 cut(s) 68, 70
AgsI TTSAA 1 cut(s) 119
AhlI ACTAGT 1 cut(s) 370
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 3 cut(s) 302, 421, 491
AlwI GGATC 4 cut(s) 176, 189, 195, 215
Aor13HI TCCGGA 1 cut(s) 184
ApeKI GCWGC 2 cut(s) 380, 652
AspLEI GCGC 2 cut(s) 549, 576
AsuC2I CCSGG 1 cut(s) 704
AsuHPI GGTGA 1 cut(s) 514
BamHI GGATCC 1 cut(s) 181
BbrPI CACGTG 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 2 cut(s) 367, 664
BccI CCATC 2 cut(s) 157, 433
BcnI CCSGG 1 cut(s) 704
BcoDI GTCTC 3 cut(s) 302, 421, 491
BcuI ACTAGT 1 cut(s) 370
BfaI CTAG 5 cut(s) 371, 431, 435, 656, 709
BisI GCNGC 7 cut(s) 138, 381, 444, 459, 577, 604, 653
BlpI GCTNAGC 1 cut(s) 447
BlsI GCNGC 7 cut(s) 139, 382, 445, 460, 578, 605, 654
Bme1390I CCNGG 1 cut(s) 704
BmiI GGNNCC 4 cut(s) 106, 146, 183, 593
BmrFI CCNGG 1 cut(s) 704
BmsI GCATC 3 cut(s) 181, 268, 445
BpmI CTGGAG 1 cut(s) 686
Bpu1102I GCTNAGC 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 1 cut(s) 704
BsaAI YACGTR 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 465, 703
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 3 cut(s) 27, 662, 692
Bsc4I CCNNNNNNNGG 1 cut(s) 514
Bse118I RCCGGY 3 cut(s) 101, 507, 599
Bse1I ACTGG 1 cut(s) 593
Bse8I GATNNNNATC 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 184
BseDI CCNNGG 2 cut(s) 465, 703
BseGI GGATG 3 cut(s) 16, 149, 444
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 1 cut(s) 514
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 2 cut(s) 596, 683
BseXI GCAGC 2 cut(s) 367, 664
BsgI GTGCAG 1 cut(s) 366
Bsh1236I CGCG 2 cut(s) 549, 576
Bsh1285I CGRYCG 1 cut(s) 319
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 543
BsiSI CCGG 5 cut(s) 102, 185, 508, 600, 703
BslI CCNNNNNNNGG 1 cut(s) 514
BsmAI GTCTC 3 cut(s) 302, 421, 491
BsmBI CGTCTC 1 cut(s) 491
Bso31I GGTCTC 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 543
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 3 cut(s) 181, 187, 207
Bsp1720I GCTNAGC 1 cut(s) 447
BspACI CCGC 9 cut(s) 40, 137, 273, 285, 443, 458, 549, 576, 603
BspCNI CTCAG 1 cut(s) 460
BspEI TCCGGA 1 cut(s) 184
BspFNI CGCG 2 cut(s) 549, 576
BspHI TCATGA 1 cut(s) 690
BspLI GGNNCC 4 cut(s) 106, 146, 183, 593
BspPI GGATC 4 cut(s) 176, 189, 195, 215
BspQI GCTCTTC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 302
BsrFI RCCGGY 3 cut(s) 101, 507, 599
BsrI ACTGG 1 cut(s) 593
BssAI RCCGGY 3 cut(s) 101, 507, 599
BssECI CCNNGG 2 cut(s) 465, 703
BssMI GATC 3 cut(s) 181, 187, 207
BssNAI GTATAC 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 465
Bst1107I GTATAC 1 cut(s) 323
Bst4CI ACNGT 2 cut(s) 320, 502
Bst6I CTCTTC 1 cut(s) 167
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 5 cut(s) 360, 509, 601, 616, 620
BstDEI CTNAG 2 cut(s) 338, 447
BstF5I GGATG 3 cut(s) 16, 149, 444
BstFNI CGCG 2 cut(s) 549, 576
BstHHI GCGC 2 cut(s) 549, 576
BstKTI GATC 3 cut(s) 184, 190, 210
BstMAI GTCTC 3 cut(s) 302, 421, 491
BstMBI GATC 3 cut(s) 181, 187, 207
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 3 cut(s) 440, 661, 670
BstNSI RCATGY 2 cut(s) 362, 547
BstSCI CCNGG 1 cut(s) 702
BstUI CGCG 2 cut(s) 549, 576
BstV1I GCAGC 2 cut(s) 367, 664
BstX2I RGATCY 2 cut(s) 181, 207
BstYI RGATCY 2 cut(s) 181, 207
BstZ17I GTATAC 1 cut(s) 323
BtsCI GGATG 3 cut(s) 16, 149, 444
BtsIMutI CAGTG 1 cut(s) 215
Cac8I GCNNGC 5 cut(s) 360, 509, 601, 616, 620
CciI TCATGA 1 cut(s) 690
CfoI GCGC 2 cut(s) 549, 576
Cfr10I RCCGGY 3 cut(s) 101, 507, 599
Csp6I GTAC 1 cut(s) 679
CviAII CATG 4 cut(s) 196, 359, 544, 691
CviQI GTAC 1 cut(s) 679
DdeI CTNAG 2 cut(s) 338, 447
DpnI GATC 3 cut(s) 183, 189, 209
DpnII GATC 3 cut(s) 181, 187, 207
DraIII CACNNNGTG 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EciI GGCGGA 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 465
Eco31I GGTCTC 1 cut(s) 302
Eco32I GATATC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 644
Eco72I CACGTG 1 cut(s) 71
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
Esp3I CGTCTC 1 cut(s) 491
FaeI CATG 4 cut(s) 199, 362, 547, 694
FaiI YATR 8 cut(s) 197, 323, 360, 395, 479, 545, 692, 698
FatI CATG 4 cut(s) 195, 358, 543, 690
FblI GTMKAC 2 cut(s) 128, 322
Fnu4HI GCNGC 7 cut(s) 138, 381, 444, 459, 577, 604, 653
FokI GGATG 3 cut(s) 23, 136, 451
Fsp4HI GCNGC 7 cut(s) 138, 381, 444, 459, 577, 604, 653
FspBI CTAG 5 cut(s) 371, 431, 435, 656, 709
GlaI GCGC 2 cut(s) 548, 575
GluI GCNGC 7 cut(s) 138, 381, 444, 459, 577, 604, 653
GsuI CTGGAG 1 cut(s) 686
HapII CCGG 5 cut(s) 102, 185, 508, 600, 703
HhaI GCGC 2 cut(s) 549, 576
Hin1II CATG 4 cut(s) 199, 362, 547, 694
Hin6I GCGC 2 cut(s) 547, 574
HinP1I GCGC 2 cut(s) 547, 574
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 3 cut(s) 47, 95, 125
HpaII CCGG 5 cut(s) 102, 185, 508, 600, 703
HphI GGTGA 1 cut(s) 514
Hpy166II GTNNAC 3 cut(s) 129, 323, 484
Hpy188I TCNGA 2 cut(s) 207, 256
Hpy188III TCNNGA 2 cut(s) 185, 691
Hpy8I GTNNAC 3 cut(s) 129, 323, 484
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 2 cut(s) 320, 502
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 362, 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 661, 670
HpyF3I CTNAG 2 cut(s) 338, 447
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 4 cut(s) 199, 362, 547, 694
HspAI GCGC 2 cut(s) 547, 574
Kpn2I TCCGGA 1 cut(s) 184
KroI GCCGGC 2 cut(s) 507, 599
KroNI GCCGGC 2 cut(s) 509, 601
Kzo9I GATC 3 cut(s) 181, 187, 207
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 7 cut(s) 110, 150, 169, 470, 571, 597, 670
Lsp1109I GCAGC 2 cut(s) 367, 664
LweI GCATC 3 cut(s) 181, 268, 445
MaeI CTAG 5 cut(s) 371, 431, 435, 656, 709
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 3 cut(s) 13, 76, 352
MalI GATC 3 cut(s) 183, 189, 209
MboI GATC 3 cut(s) 181, 187, 207
MboII GAAGA 3 cut(s) 32, 154, 637
MflI RGATCY 2 cut(s) 181, 207
MhlI GDGCHC 1 cut(s) 543
MluCI AATT 1 cut(s) 345
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 5 cut(s) 65, 412, 574, 577, 661
MroI TCCGGA 1 cut(s) 184
MroNI GCCGGC 2 cut(s) 507, 599
MspA1I CMGCKG 1 cut(s) 622
MspI CCGG 5 cut(s) 102, 185, 508, 600, 703
MspR9I CCNGG 1 cut(s) 704
MvnI CGCG 2 cut(s) 549, 576
MwoI GCNNNNNNNGC 3 cut(s) 440, 661, 670
NaeI GCCGGC 2 cut(s) 509, 601
NciI CCSGG 1 cut(s) 704
NdeII GATC 3 cut(s) 181, 187, 207
NgoMIV GCCGGC 2 cut(s) 507, 599
NlaIII CATG 4 cut(s) 199, 362, 547, 694
NlaIV GGNNCC 4 cut(s) 106, 146, 183, 593
NmeAIII GCCGAG 2 cut(s) 121, 499
NmuCI GTSAC 2 cut(s) 76, 352
NspI RCATGY 2 cut(s) 362, 547
PaeI GCATGC 1 cut(s) 362
PagI TCATGA 1 cut(s) 690
PciSI GCTCTTC 1 cut(s) 167
PdiI GCCGGC 2 cut(s) 509, 601
PfeI GAWTC 2 cut(s) 47, 95
PkrI GCNGC 7 cut(s) 139, 382, 445, 460, 578, 605, 654
PleI GAGTC 1 cut(s) 133
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
PpsI GAGTC 1 cut(s) 133
Ppu21I YACGTR 1 cut(s) 71
PspCI CACGTG 1 cut(s) 71
PspN4I GGNNCC 4 cut(s) 106, 146, 183, 593
PsuI RGATCY 2 cut(s) 181, 207
PvuII CAGCTG 1 cut(s) 622
RsaI GTAC 1 cut(s) 680
RsaNI GTAC 1 cut(s) 679
SalI GTCGAC 1 cut(s) 127
SapI GCTCTTC 1 cut(s) 167
SatI GCNGC 7 cut(s) 138, 381, 444, 459, 577, 604, 653
Sau3AI GATC 3 cut(s) 181, 187, 207
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 704
SduI GDGCHC 1 cut(s) 543
SfaNI GCATC 3 cut(s) 181, 268, 445
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
SpeI ACTAGT 1 cut(s) 370
SphI GCATGC 1 cut(s) 362
Sse9I AATT 1 cut(s) 345
SsiI CCGC 9 cut(s) 40, 137, 273, 285, 443, 458, 549, 576, 603
SspMI CTAG 5 cut(s) 371, 431, 435, 656, 709
StyD4I CCNGG 1 cut(s) 702
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 2 cut(s) 320, 502
TaiI ACGT 1 cut(s) 73
TaqI TCGA 5 cut(s) 57, 128, 315, 408, 453
TasI AATT 1 cut(s) 345
TauI GCSGC 5 cut(s) 140, 446, 461, 579, 606
TfiI GAWTC 2 cut(s) 47, 95
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 2 cut(s) 76, 352
TseI GCWGC 2 cut(s) 380, 652
Tsp45I GTSAC 2 cut(s) 76, 352
TspDTI ATGAA 1 cut(s) 679
TspRI CASTG 1 cut(s) 222
XceI RCATGY 2 cut(s) 362, 547
XmiI GTMKAC 2 cut(s) 128, 322
XspI CTAG 5 cut(s) 371, 431, 435, 656, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.