FvH4_7g30010

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006589
Physical Location & Seq
Reverse (-)
21941404 .. 21942922
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30010.t2

Sequence Viewer

Length: 1104 bp
ATGAGACAAAAACTTAGTGCACGCATCCGTAAATTGATTTCTTGTACAGCATCAAGACCCGTCATCACACCAAATTCTTCTATGGGAGTGGATGTCACAGAAGAATACGCCAATGCCTTTCGCACCGAATCATACCTCGACTTTTGGACGCGTGTCGTTGCGCTATCCAATGCCGGTTCTACCGGCTCCAAGCTTATTCAAATGGAGTCGACAACTGCGGCTCGGCTCTCATCATATCGGCTCTTCGTGGAGAACCTCTTGGATCCGGATCAAACCATCGTTACTCGGATCTTGGCAATGACCCGAAACCACCCAAAGAGCTTTGCACTTCTATCCGACTATTTTACCCAAACCGCAGACGCTTCCGCCCTATGTGGCCTCCTACTAAAAGATATCGACCGGACACGTGTCAAATACCGTTCCATCAGAACCGCACTTCAATCTCTAAATAACTGTCTCACTCCTACGACACTGAACCAGCTAACTAAATTCTCCATCATTTTATCTCCCATACGGATTCAAAGCATACAAGCTGGATGTTCCAATTTGCTAAAACGCCTAGAATTGACTCGCAACAAGACTAGAGCCAAACTAAGGCTCGCAGGCAAATTCCAAACTGCCTCGGCTATCTTTTTAGTGGCAGTGACGGCTACACTAAGTGTAATTATTGTCACGCATGCACTTGCCCTAGTGGTGGCTGCACCAACTCTTATAGCAACTTCACTCGACTTGACCTCGTCGAGGAGACTAGCTAGGATGGCGACGCAGCTCGACGCGGCCACCAAGGGAGCTTACATAGTAAGCAGGGATTTGGAGACGGTGAGCAGGCTCATGGCTAGGTTAAACGACGAGCTTGAGCACATGCGCGGTATGGTTCAGTTTTGGGTGGAGCGCGCGGAGGAGGACTGGCTCCAACCCGGCGGAGAAGTGGCGCGGCAGCTGAAGAAAAATGATTGTAGCTTTAGACAGAAGCTAGATGAGCTAGAGGAGCATTTGTATCTGTGTTTCATGAATATAAACCGGGCTAGAAATCTAGTAGTGAAGGAGATTCTGGATCCGGGTCAGAACACCACCAATTCCCATGCCACATTGGTAATGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

368

Amino Acids

41.07

Weight (kDa)

9.71

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 41 - 323 6.1e-14 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 209
AccII CGCG 6 cut(s) 151, 776, 867, 894, 896, 934
AccIII TCCGGA 1 cut(s) 265
AciI CCGC 9 cut(s) 218, 354, 366, 432, 776, 867, 896, 921, 934
AclWI GGATC 6 cut(s) 257, 270, 276, 296, 1049, 1062
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 3 cut(s) 73, 488, 608
AcuI CTGAAG 1 cut(s) 962
AcvI CACGTG 1 cut(s) 407
AdeI CACNNNGTG 1 cut(s) 659
AfaI GTAC 1 cut(s) 46
AfiI CCNNNNNNNGG 3 cut(s) 594, 694, 741
AflIII ACRYGT 3 cut(s) 149, 404, 406
AgsI TTSAA 3 cut(s) 200, 440, 521
Alw21I GWGCWC 2 cut(s) 22, 861
Alw26I GTCTC 3 cut(s) 461, 739, 809
Alw44I GTGCAC 1 cut(s) 18
AlwI GGATC 6 cut(s) 257, 270, 276, 296, 1049, 1062
Aor13HI TCCGGA 1 cut(s) 265
AoxI GGCC 2 cut(s) 376, 777
ApaLI GTGCAC 1 cut(s) 18
ApeKI GCWGC 3 cut(s) 698, 766, 937
ApoI RAATTY 3 cut(s) 73, 488, 608
AspLEI GCGC 5 cut(s) 163, 867, 894, 896, 934
AsuC2I CCSGG 3 cut(s) 918, 1022, 1059
AsuHPI GGTGA 1 cut(s) 832
BaeGI GKGCMC 1 cut(s) 22
BamHI GGATCC 2 cut(s) 262, 1054
BbrPI CACGTG 1 cut(s) 407
Bbv12I GWGCWC 2 cut(s) 22, 861
BbvI GCAGC 3 cut(s) 685, 778, 949
BccI CCATC 4 cut(s) 284, 431, 503, 751
BceAI ACGGC 1 cut(s) 663
BcnI CCSGG 3 cut(s) 918, 1022, 1059
BcoDI GTCTC 3 cut(s) 461, 739, 809
BisI GCNGC 6 cut(s) 219, 699, 767, 777, 935, 938
BlsI GCNGC 6 cut(s) 220, 700, 768, 778, 936, 939
Bme1390I CCNGG 3 cut(s) 918, 1022, 1059
BmiI GGNNCC 4 cut(s) 187, 264, 911, 1056
BmrFI CCNGG 3 cut(s) 918, 1022, 1059
BmsI GCATC 2 cut(s) 33, 59
BoxI GACNNNNGTC 2 cut(s) 152, 407
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 3 cut(s) 918, 1022, 1059
BsaAI YACGTR 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 621, 783
BsaWI WCCGGW 2 cut(s) 265, 399
BsaXI ACNNNNNCTCC 4 cut(s) 78, 108, 980, 1010
Bsc4I CCNNNNNNNGG 3 cut(s) 594, 694, 741
Bse118I RCCGGY 2 cut(s) 173, 182
Bse1I ACTGG 1 cut(s) 911
Bse3DI GCAATG 1 cut(s) 303
Bse8I GATNNNNATC 1 cut(s) 267
BseAI TCCGGA 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 621, 783
BseGI GGATG 4 cut(s) 24, 97, 542, 762
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 594, 694, 741
BseMI GCAATG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 911
BsePI GCGCGC 1 cut(s) 892
BseRI GAGGAG 3 cut(s) 757, 914, 1001
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 3 cut(s) 685, 778, 949
BsgI GTGCAG 1 cut(s) 684
Bsh1236I CGCG 6 cut(s) 151, 776, 867, 894, 896, 934
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 2 cut(s) 378, 779
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 2 cut(s) 22, 861
BsiSI CCGG 7 cut(s) 174, 183, 266, 400, 918, 1021, 1058
BslI CCNNNNNNNGG 3 cut(s) 594, 694, 741
BsmAI GTCTC 3 cut(s) 461, 739, 809
BsmBI CGTCTC 1 cut(s) 809
BsnI GGCC 2 cut(s) 378, 779
Bsp1286I GDGCHC 2 cut(s) 22, 861
Bsp13I TCCGGA 1 cut(s) 265
Bsp1407I TGTACA 1 cut(s) 44
Bsp143I GATC 4 cut(s) 262, 268, 288, 1054
BspACI CCGC 9 cut(s) 218, 354, 366, 432, 776, 867, 896, 921, 934
BspANI GGCC 2 cut(s) 378, 779
BspEI TCCGGA 1 cut(s) 265
BspFNI CGCG 6 cut(s) 151, 776, 867, 894, 896, 934
BspHI TCATGA 1 cut(s) 1008
BspLI GGNNCC 4 cut(s) 187, 264, 911, 1056
BspPI GGATC 6 cut(s) 257, 270, 276, 296, 1049, 1062
BspQI GCTCTTC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 303
BsrFI RCCGGY 2 cut(s) 173, 182
BsrGI TGTACA 1 cut(s) 44
BsrI ACTGG 1 cut(s) 911
BssAI RCCGGY 2 cut(s) 173, 182
BssECI CCNNGG 2 cut(s) 621, 783
BssHII GCGCGC 1 cut(s) 892
BssMI GATC 4 cut(s) 262, 268, 288, 1054
BssT1I CCWWGG 1 cut(s) 783
Bst4CI ACNGT 3 cut(s) 419, 455, 820
Bst6I CTCTTC 1 cut(s) 248
BstAUI TGTACA 1 cut(s) 44
BstBAI YACGTR 1 cut(s) 407
BstC8I GCNNGC 6 cut(s) 22, 600, 604, 678, 827, 894
BstDEI CTNAG 3 cut(s) 14, 593, 656
BstENI CCTNNNNNAGG 1 cut(s) 739
BstF5I GGATG 4 cut(s) 24, 97, 542, 762
BstFNI CGCG 6 cut(s) 151, 776, 867, 894, 896, 934
BstHHI GCGC 5 cut(s) 163, 867, 894, 896, 934
BstKTI GATC 4 cut(s) 265, 271, 291, 1057
BstMAI GTCTC 3 cut(s) 461, 739, 809
BstMBI GATC 4 cut(s) 262, 268, 288, 1054
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 4 cut(s) 647, 758, 979, 988
BstNSI RCATGY 2 cut(s) 680, 865
BstPAI GACNNNNGTC 2 cut(s) 152, 407
BstSCI CCNGG 3 cut(s) 916, 1020, 1057
BstSLI GKGCMC 1 cut(s) 22
BstUI CGCG 6 cut(s) 151, 776, 867, 894, 896, 934
BstV1I GCAGC 3 cut(s) 685, 778, 949
BstX2I RGATCY 3 cut(s) 262, 288, 1054
BstYI RGATCY 3 cut(s) 262, 288, 1054
BsuRI GGCC 2 cut(s) 378, 779
BtsCI GGATG 4 cut(s) 24, 97, 542, 762
BtsI GCAGTG 1 cut(s) 648
BtsIMutI CAGTG 2 cut(s) 470, 648
Cac8I GCNNGC 6 cut(s) 22, 600, 604, 678, 827, 894
CciI TCATGA 1 cut(s) 1008
CfoI GCGC 5 cut(s) 163, 867, 894, 896, 934
Cfr10I RCCGGY 2 cut(s) 173, 182
CseI GACGC 4 cut(s) 157, 368, 772, 782
Csp6I GTAC 1 cut(s) 45
CviAII CATG 5 cut(s) 677, 832, 862, 1009, 1082
CviQI GTAC 1 cut(s) 45
DdeI CTNAG 3 cut(s) 14, 593, 656
DpnI GATC 4 cut(s) 264, 270, 290, 1056
DpnII GATC 4 cut(s) 262, 268, 288, 1054
DraIII CACNNNGTG 1 cut(s) 659
EaeI YGGCCR 1 cut(s) 777
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
EciI GGCGGA 2 cut(s) 355, 936
Eco130I CCWWGG 1 cut(s) 783
Eco32I GATATC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 962
Eco72I CACGTG 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 739
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 783
Esp3I CGTCTC 1 cut(s) 809
FaeI CATG 5 cut(s) 680, 835, 865, 1012, 1085
FatI CATG 5 cut(s) 676, 831, 861, 1008, 1081
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 6 cut(s) 219, 699, 767, 777, 935, 938
FokI GGATG 4 cut(s) 11, 104, 549, 769
Fsp4HI GCNGC 6 cut(s) 219, 699, 767, 777, 935, 938
GlaI GCGC 5 cut(s) 162, 866, 893, 895, 933
GluI GCNGC 6 cut(s) 219, 699, 767, 777, 935, 938
HaeIII GGCC 2 cut(s) 378, 779
HapII CCGG 7 cut(s) 174, 183, 266, 400, 918, 1021, 1058
HgaI GACGC 4 cut(s) 157, 368, 772, 782
HhaI GCGC 5 cut(s) 163, 867, 894, 896, 934
Hin1II CATG 5 cut(s) 680, 835, 865, 1012, 1085
Hin6I GCGC 5 cut(s) 161, 865, 892, 894, 932
HinP1I GCGC 5 cut(s) 161, 865, 892, 894, 932
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 5 cut(s) 128, 206, 517, 568, 1048
HpaII CCGG 7 cut(s) 174, 183, 266, 400, 918, 1021, 1058
HphI GGTGA 1 cut(s) 832
Hpy166II GTNNAC 2 cut(s) 20, 210
Hpy188I TCNGA 4 cut(s) 288, 337, 428, 1065
Hpy188III TCNNGA 4 cut(s) 54, 266, 1009, 1052
Hpy8I GTNNAC 2 cut(s) 20, 210
Hpy99I CGWCG 4 cut(s) 742, 766, 776, 851
HpyAV CCTTC 1 cut(s) 1036
HpyCH4III ACNGT 3 cut(s) 419, 455, 820
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 4 cut(s) 20, 326, 680, 701
HpyF10VI GCNNNNNNNGC 4 cut(s) 647, 758, 979, 988
HpyF3I CTNAG 3 cut(s) 14, 593, 656
HpySE526I ACGT 1 cut(s) 406
Hsp92II CATG 5 cut(s) 680, 835, 865, 1012, 1085
HspAI GCGC 5 cut(s) 161, 865, 892, 894, 932
Kpn2I TCCGGA 1 cut(s) 265
Kzo9I GATC 4 cut(s) 262, 268, 288, 1054
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 5 cut(s) 191, 788, 889, 915, 988
Lsp1109I GCAGC 3 cut(s) 685, 778, 949
LweI GCATC 2 cut(s) 33, 59
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 4 cut(s) 94, 280, 643, 670
MalI GATC 4 cut(s) 264, 270, 290, 1056
MboI GATC 4 cut(s) 262, 268, 288, 1054
MboII GAAGA 4 cut(s) 69, 113, 235, 955
MflI RGATCY 3 cut(s) 262, 288, 1054
MhlI GDGCHC 2 cut(s) 22, 861
MluCI AATT 8 cut(s) 32, 73, 488, 544, 563, 608, 663, 1075
MluI ACGCGT 1 cut(s) 149
MlyI GAGTC 2 cut(s) 215, 562
MmeI TCCRAC 2 cut(s) 360, 937
MnlI CCTC 9 cut(s) 146, 266, 389, 631, 735, 745, 892, 895, 979
MroI TCCGGA 1 cut(s) 265
MseI TTAA 1 cut(s) 842
MspA1I CMGCKG 1 cut(s) 940
MspI CCGG 7 cut(s) 174, 183, 266, 400, 918, 1021, 1058
MspR9I CCNGG 3 cut(s) 918, 1022, 1059
MvnI CGCG 6 cut(s) 151, 776, 867, 894, 896, 934
MwoI GCNNNNNNNGC 4 cut(s) 647, 758, 979, 988
NciI CCSGG 3 cut(s) 918, 1022, 1059
NdeII GATC 4 cut(s) 262, 268, 288, 1054
NlaIII CATG 5 cut(s) 680, 835, 865, 1012, 1085
NlaIV GGNNCC 4 cut(s) 187, 264, 911, 1056
NmeAIII GCCGAG 2 cut(s) 202, 602
NmuCI GTSAC 3 cut(s) 94, 643, 670
NspI RCATGY 2 cut(s) 680, 865
PaeI GCATGC 1 cut(s) 680
PagI TCATGA 1 cut(s) 1008
PauI GCGCGC 1 cut(s) 892
PciSI GCTCTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 128, 517, 1048
PflFI GACNNNGTC 1 cut(s) 736
PkrI GCNGC 6 cut(s) 220, 700, 768, 778, 936, 939
PleI GAGTC 2 cut(s) 214, 562
PmaCI CACGTG 1 cut(s) 407
PmlI CACGTG 1 cut(s) 407
PpsI GAGTC 2 cut(s) 214, 562
Ppu21I YACGTR 1 cut(s) 407
PshAI GACNNNNGTC 2 cut(s) 152, 407
PspCI CACGTG 1 cut(s) 407
PspN4I GGNNCC 4 cut(s) 187, 264, 911, 1056
PsuI RGATCY 3 cut(s) 262, 288, 1054
PsyI GACNNNGTC 1 cut(s) 736
PteI GCGCGC 1 cut(s) 892
PvuII CAGCTG 1 cut(s) 940
RsaI GTAC 1 cut(s) 46
RsaNI GTAC 1 cut(s) 45
SalI GTCGAC 1 cut(s) 208
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 842
SatI GCNGC 6 cut(s) 219, 699, 767, 777, 935, 938
Sau3AI GATC 4 cut(s) 262, 268, 288, 1054
SchI GAGTC 2 cut(s) 215, 562
ScrFI CCNGG 3 cut(s) 918, 1022, 1059
SduI GDGCHC 2 cut(s) 22, 861
SfaNI GCATC 2 cut(s) 33, 59
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
SphI GCATGC 1 cut(s) 680
Sse9I AATT 8 cut(s) 32, 73, 488, 544, 563, 608, 663, 1075
SsiI CCGC 9 cut(s) 218, 354, 366, 432, 776, 867, 896, 921, 934
StyD4I CCNGG 3 cut(s) 916, 1020, 1057
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 3 cut(s) 419, 455, 820
TaiI ACGT 1 cut(s) 409
TaqI TCGA 6 cut(s) 138, 209, 396, 726, 740, 771
TasI AATT 8 cut(s) 32, 73, 488, 544, 563, 608, 663, 1075
TatI WGTACW 1 cut(s) 44
TauI GCSGC 3 cut(s) 221, 779, 937
TfiI GAWTC 3 cut(s) 128, 517, 1048
Tru1I TTAA 1 cut(s) 842
Tru9I TTAA 1 cut(s) 842
TscAI CASTG 2 cut(s) 477, 648
TseFI GTSAC 3 cut(s) 94, 643, 670
TseI GCWGC 3 cut(s) 698, 766, 937
Tsp45I GTSAC 3 cut(s) 94, 643, 670
TspDTI ATGAA 2 cut(s) 997, 1025
TspGWI ACGGA 2 cut(s) 17, 529
TspRI CASTG 2 cut(s) 477, 648
Tth111I GACNNNGTC 1 cut(s) 736
VneI GTGCAC 1 cut(s) 18
XagI CCTNNNNNAGG 1 cut(s) 739
XapI RAATTY 3 cut(s) 73, 488, 608
XceI RCATGY 2 cut(s) 680, 865
XmiI GTMKAC 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.