AT3G50050

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008276
Physical Location & Seq
Reverse (-)
18553941 .. 18557460
3520 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50050.1

Sequence Viewer

Length: 1899 bp
ATGGCACTGCCGTCGATCTCATCAATCGGCGCGACGTTCTCACTCCTAATCTATTTATCTCTTCCTTATTCAATCACAGCTGGTGAAAACAACCTCCTCCACCAATCTCCAACCGCTAGATCACGACGTCCCATGGTGTTCCCATTGTTCCTCTCTCAACCAAATTCTTCTTCGAGATCCATATCAATCCCACATCGAAAACTCCACAAATCCGACTCAAAATCACTTCCTCACTCTCGCATGAGACTCTACGACGATCTTCTTATTAATGGGTATTACACTACGAGGCTTTGGATTGGTACACCACCACAAATGTTTGCTCTTATAGTTGATTCTGGTAGTACTGTTACTTATGTTCCTTGTTCTGATTGTGAACAATGTGGCAAACACCAGGACCCAAAATTTCAGCCAGAAATGTCTAGCACTTATCAACCTGTTAAGTGTAACATGGATTGTAATTGTGATGATGATAGAGAACAATGTGTTTACGAGAGAGAGTATGCTGAACATAGTAGTAGTAAAGGTGTTCTTGGAGAAGATCTTATTTCATTTGGCAATGAAAGCCAGCTAACACCTCAACGAGCTGTTTTCGGTTGTGAAACTGTTGAAACCGGTGATCTTTATAGTCAACGTGCTGATGGGATTATTGGATTAGGTCAAGGAGATCTTAGTCTTGTTGATCAATTGGTGGATAAAGGTTTGATAAGCAACTCTTTTGGTTTGTGTTATGGAGGGATGGATGTTGGTGGTGGTTCGATGATTCTTGGCGGTTTTGATTATCCATCTGATATGGTGTTTACGGACTCGGACCCTGATCGTAGTCCATATTACAATATAGATTTGACGGGGATACGTGTTGCTGGGAAGCAGTTGTCTCTTCATTCGAGAGTCTTTGACGGGGAACATGGCGCCGTACTAGACAGTGGTACAACATATGCTTATCTTCCAGACGCAGCATTTGCGGCATTTGAGGAAGCTGTAATGAGAGAAGTTTCCACATTGAAGCAGATTGATGGTCCTGATCCAAATTTTAAAGATACTTGCTTCCAAGTCGCTGCAAGTAATTATGTCTCGGAACTTTCAAAGATATTCCCATCGGTCGAGATGGTATTTAAGAGTGGACAGTCATGGCTGTTGTCCCCTGAAAATTACATGTTTCGACATTCCAAGGTGCATGGTGCTTACTGTCTCGGTGTCTTCCCAAACGGAAAAGATCACACAACTCTTTTAGGAGGAATTGTGGTTCGTAATACACTTGTTGTGTATGATCGTGAGAATTCGAAGGTTGGATTCTGGAGAACCAATTGTTCGGAGTTATCGGATAGGCTTCATATTGATGGTGCACCACCACCTGCTACTTTGCCTTCAAATGATTCAAATCCCTCCCACAATTCATCAAGCAATCTGTCAGGTGTGACTCAAGTTGGTCAAATAAACCTTGATATTCAACTAACAGTCAACTCGTCCTATCTGAAACCTCGGATTGAAGATCTCTCCAAGATATTCTCCAAGGAGCTCGATGTCAAATCTTCACAGGTCAGTTTATCGAATCTCACCTCCAAAGGAAACGAGTCTCTCGTCAGAATGGTTGTCCTCCCACCTGAACCTTCTACTTGGTTTTCAAATGTCACAGCAACGAATATAGTTTCTCGGTTTACAAATCATCAAATAAAGCTTCCTGAAATCTTCGGAAACTATCAGCTGGTTAATTACAAACTCGAGCCTCCAAGAAAACGGACGAATAACAACATTGTCGTGATCGCGATTGGAATCATCGCTGTAATCGTCGGGTTATCAGCTTATGGAGCTTGGTTGATATGGAAACGGAAACAAACATCAATTCCATACAAGCCTGTTGATGAAGCTATAGTAGCTGAGCAAGAACTTCAACCTATATAA

Protein Analysis

632

Amino Acids

69.75

Weight (kDa)

5.59

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 93 - 257 6e-36 Xylanase inhibitor N-terminal
Asp PF00026 93 - 431 3.3e-33 Eukaryotic aspartyl protease
TAXi_C PF14541 277 - 431 8.8e-28 Xylanase inhibitor C-terminal
ALE2_N PF23180 471 - 571 1.9e-07 Receptor-like PK ALE2 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1360
AatII GACGTC 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 1360
AccB1I GGYRCC 1 cut(s) 908
AccII CGCG 2 cut(s) 32, 1763
AciI CCGC 3 cut(s) 114, 768, 962
AclWI GGATC 2 cut(s) 171, 1016
AcsI RAATTY 4 cut(s) 163, 401, 1027, 1276
AcyI GRCGYC 2 cut(s) 127, 909
AfaI GTAC 4 cut(s) 301, 343, 915, 928
AflIII ACRYGT 2 cut(s) 853, 1152
AgeI ACCGGT 1 cut(s) 611
AjnI CCWGG 1 cut(s) 390
AjuI GAANNNNNNNTTGG 2 cut(s) 154, 186
AloI GAACNNNNNNTCC 2 cut(s) 1227, 1259
Alw21I GWGCWC 2 cut(s) 1345, 1518
Alw26I GTCTC 5 cut(s) 238, 879, 1075, 1193, 1578
Alw44I GTGCAC 1 cut(s) 1341
AlwI GGATC 2 cut(s) 171, 1016
Ama87I CYCGRG 1 cut(s) 1718
ApaLI GTGCAC 1 cut(s) 1341
ApeKI GCWGC 2 cut(s) 953, 1055
ApoI RAATTY 4 cut(s) 163, 401, 1027, 1276
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
AseI ATTAAT 1 cut(s) 267
AsiGI ACCGGT 1 cut(s) 611
AspLEI GCGC 2 cut(s) 32, 911
AspS9I GGNCC 3 cut(s) 394, 808, 1016
AsuHPI GGTGA 3 cut(s) 95, 626, 1546
AsuII TTCGAA 1 cut(s) 1280
AvaI CYCGRG 1 cut(s) 1718
AvaII GGWCC 3 cut(s) 394, 808, 1016
BaeGI GKGCMC 1 cut(s) 1345
BanI GGYRCC 1 cut(s) 908
BanII GRGCYC 1 cut(s) 1518
BbsI GAAGAC 1 cut(s) 1189
Bbv12I GWGCWC 2 cut(s) 1345, 1518
BbvI GCAGC 2 cut(s) 965, 1042
BccI CCATC 7 cut(s) 632, 730, 790, 1007, 1099, 1102, 1331
BceAI ACGGC 1 cut(s) 896
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 392
BciVI GTATCC 1 cut(s) 843
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 5 cut(s) 238, 879, 1075, 1193, 1578
BfaI CTAG 3 cut(s) 117, 420, 917
BfmI CTRYAG 1 cut(s) 1866
BfoI RGCGCY 1 cut(s) 912
BfuAI ACCTGC 1 cut(s) 1360
BfuI GTATCC 1 cut(s) 843
BglII AGATCT 3 cut(s) 538, 664, 1489
BisI GCNGC 3 cut(s) 954, 963, 1056
BlpI GCTNAGC 1 cut(s) 1875
BlsI GCNGC 3 cut(s) 955, 964, 1057
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 3 cut(s) 394, 808, 1016
BmeT110I CYCGRG 1 cut(s) 1718
BmgT120I GGNCC 3 cut(s) 394, 808, 1016
BmiI GGNNCC 3 cut(s) 396, 810, 910
BmrFI CCNGG 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 1189
BpmI CTGGAG 1 cut(s) 1315
Bpu1102I GCTNAGC 1 cut(s) 1875
Bpu14I TTCGAA 1 cut(s) 1280
BpuEI CTTGAG 1 cut(s) 1404
BsaAI YACGTR 1 cut(s) 854
BsaBI GATNNNNATC 3 cut(s) 181, 1377, 1769
BsaHI GRCGYC 2 cut(s) 127, 909
BsaJI CCNNGG 4 cut(s) 132, 1167, 1478, 1509
BsaWI WCCGGW 1 cut(s) 611
BsaXI ACNNNNNCTCC 2 cut(s) 1224, 1254
Bse118I RCCGGY 1 cut(s) 611
Bse3DI GCAATG 1 cut(s) 562
Bse8I GATNNNNATC 3 cut(s) 181, 1377, 1769
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 4 cut(s) 132, 1167, 1478, 1509
BseGI GGATG 2 cut(s) 741, 745
BseJI GATNNNNATC 3 cut(s) 181, 1377, 1769
BseMI GCAATG 1 cut(s) 562
BseMII CTCAG 1 cut(s) 1866
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 1345
BseXI GCAGC 2 cut(s) 965, 1042
BseYI CCCAGC 1 cut(s) 860
Bsh1236I CGCG 2 cut(s) 32, 1763
Bsh1285I CGRYCG 1 cut(s) 1101
BshNI GGYRCC 1 cut(s) 908
BshTI ACCGGT 1 cut(s) 611
BsiEI CGRYCG 1 cut(s) 1101
BsiHKAI GWGCWC 2 cut(s) 1345, 1518
BsiHKCI CYCGRG 1 cut(s) 1718
BsiSI CCGG 1 cut(s) 612
BslFI GGGAC 2 cut(s) 114, 1123
BsmAI GTCTC 5 cut(s) 238, 879, 1075, 1193, 1578
BsmFI GGGAC 2 cut(s) 114, 1123
BsoBI CYCGRG 1 cut(s) 1718
Bsp119I TTCGAA 1 cut(s) 1280
Bsp1286I GDGCHC 2 cut(s) 1345, 1518
Bsp1720I GCTNAGC 1 cut(s) 1875
Bsp19I CCATGG 1 cut(s) 132
Bsp68I TCGCGA 1 cut(s) 1763
BspACI CCGC 3 cut(s) 114, 768, 962
BspCNI CTCAG 1 cut(s) 1867
BspFNI CGCG 2 cut(s) 32, 1763
BspLI GGNNCC 3 cut(s) 396, 810, 910
BspMI ACCTGC 1 cut(s) 1360
BspPI GGATC 2 cut(s) 171, 1016
BspT104I TTCGAA 1 cut(s) 1280
BspT107I GGYRCC 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 562
BsrFI RCCGGY 1 cut(s) 611
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 4 cut(s) 132, 1167, 1478, 1509
BssNI GRCGYC 2 cut(s) 127, 909
BssT1I CCWWGG 3 cut(s) 132, 1167, 1509
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 6 cut(s) 346, 604, 923, 1125, 1187, 1456
Bst6I CTCTTC 2 cut(s) 66, 882
BstACI GRCGYC 2 cut(s) 127, 909
BstAPI GCANNNNNTGC 1 cut(s) 959
BstBAI YACGTR 1 cut(s) 854
BstBI TTCGAA 1 cut(s) 1280
BstC8I GCNNGC 1 cut(s) 566
BstDEI CTNAG 2 cut(s) 668, 1875
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 2 cut(s) 741, 745
BstFNI CGCG 2 cut(s) 32, 1763
BstH2I RGCGCY 1 cut(s) 912
BstHHI GCGC 2 cut(s) 32, 911
BstMAI GTCTC 5 cut(s) 238, 879, 1075, 1193, 1578
BstMCI CGRYCG 1 cut(s) 1101
BstMWI GCNNNNNNNGC 5 cut(s) 561, 959, 962, 1805, 1871
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 1 cut(s) 1156
BstSCI CCNGG 1 cut(s) 390
BstSFI CTRYAG 1 cut(s) 1866
BstSLI GKGCMC 1 cut(s) 1345
BstUI CGCG 2 cut(s) 32, 1763
BstV1I GCAGC 2 cut(s) 965, 1042
BstV2I GAAGAC 1 cut(s) 1189
BstX2I RGATCY 4 cut(s) 176, 538, 664, 1489
BstYI RGATCY 4 cut(s) 176, 538, 664, 1489
BsuI GTATCC 1 cut(s) 843
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 1759
BtsCI GGATG 2 cut(s) 741, 745
BtsI GCAGTG 1 cut(s) 5
BtsIMutI CAGTG 2 cut(s) 5, 928
BtuMI TCGCGA 1 cut(s) 1763
BveI ACCTGC 1 cut(s) 1360
Cac8I GCNNGC 1 cut(s) 566
CfoI GCGC 2 cut(s) 32, 911
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 3 cut(s) 394, 808, 1016
CseI GACGC 1 cut(s) 959
Csp6I GTAC 4 cut(s) 300, 342, 914, 927
CspAI ACCGGT 1 cut(s) 611
CviAII CATG 7 cut(s) 133, 241, 448, 905, 1128, 1153, 1175
CviQI GTAC 4 cut(s) 300, 342, 914, 927
DdeI CTNAG 2 cut(s) 668, 1875
DinI GGCGCC 1 cut(s) 910
DraI TTTAAA 1 cut(s) 1033
Eam1104I CTCTTC 2 cut(s) 66, 882
EarI CTCTTC 2 cut(s) 66, 882
Ecl136II GAGCTC 1 cut(s) 1516
Eco130I CCWWGG 3 cut(s) 132, 1167, 1509
Eco24I GRGCYC 1 cut(s) 1518
Eco47I GGWCC 3 cut(s) 394, 808, 1016
Eco53kI GAGCTC 1 cut(s) 1516
Eco88I CYCGRG 1 cut(s) 1718
EcoICRI GAGCTC 1 cut(s) 1516
EcoO109I RGGNCCY 1 cut(s) 394
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 3 cut(s) 132, 1167, 1509
EcoT38I GRGCYC 1 cut(s) 1518
EgeI GGCGCC 1 cut(s) 910
EheI GGCGCC 1 cut(s) 910
ErhI CCWWGG 3 cut(s) 132, 1167, 1509
FaeI CATG 7 cut(s) 136, 244, 451, 908, 1131, 1156, 1178
FalI AAGNNNNNCTT 6 cut(s) 513, 545, 651, 683, 697, 729
FaqI GGGAC 2 cut(s) 114, 1123
FatI CATG 7 cut(s) 132, 240, 447, 904, 1127, 1152, 1174
FauNDI CATATG 1 cut(s) 934
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 3 cut(s) 954, 963, 1056
FokI GGATG 2 cut(s) 748, 752
FriOI GRGCYC 1 cut(s) 1518
Fsp4HI GCNGC 3 cut(s) 954, 963, 1056
FspBI CTAG 3 cut(s) 117, 420, 917
GlaI GCGC 2 cut(s) 31, 910
GluI GCNGC 3 cut(s) 954, 963, 1056
GsaI CCCAGC 1 cut(s) 864
GsuI CTGGAG 1 cut(s) 1315
HaeII RGCGCY 1 cut(s) 912
HapII CCGG 1 cut(s) 612
HgaI GACGC 1 cut(s) 959
HhaI GCGC 2 cut(s) 32, 911
Hin1I GRCGYC 2 cut(s) 127, 909
Hin1II CATG 7 cut(s) 136, 244, 451, 908, 1131, 1156, 1178
Hin6I GCGC 2 cut(s) 30, 909
HinP1I GCGC 2 cut(s) 30, 909
HincII GTYRAC 2 cut(s) 629, 1459
HindII GTYRAC 2 cut(s) 629, 1459
HindIII AAGCTT 1 cut(s) 1673
HpaII CCGG 1 cut(s) 612
HphI GGTGA 3 cut(s) 95, 626, 1546
Hpy166II GTNNAC 9 cut(s) 302, 374, 487, 629, 798, 1121, 1343, 1459, 1656
Hpy8I GTNNAC 9 cut(s) 302, 374, 487, 629, 798, 1121, 1343, 1459, 1656
Hpy99I CGWCG 5 cut(s) 16, 37, 129, 257, 1790
HpyAV CCTTC 3 cut(s) 1276, 1374, 1617
HpyCH4III ACNGT 6 cut(s) 346, 604, 923, 1125, 1187, 1456
HpyCH4IV ACGT 4 cut(s) 35, 127, 631, 853
HpyCH4V TGCA 3 cut(s) 1058, 1174, 1343
HpyF10VI GCNNNNNNNGC 5 cut(s) 561, 959, 962, 1805, 1871
HpyF3I CTNAG 2 cut(s) 668, 1875
HpySE526I ACGT 4 cut(s) 35, 127, 631, 853
Hsp92I GRCGYC 2 cut(s) 127, 909
Hsp92II CATG 7 cut(s) 136, 244, 451, 908, 1131, 1156, 1178
HspAI GCGC 2 cut(s) 30, 909
KasI GGCGCC 1 cut(s) 908
Ksp22I TGATCA 1 cut(s) 679
LmnI GCTCC 2 cut(s) 1513, 1805
Lsp1109I GCAGC 2 cut(s) 965, 1042
MaeI CTAG 3 cut(s) 117, 420, 917
MaeII ACGT 4 cut(s) 35, 127, 631, 853
MaeIII GTNAC 4 cut(s) 346, 443, 1414, 1627
MfeI CAATTG 2 cut(s) 683, 1303
MflI RGATCY 4 cut(s) 176, 538, 664, 1489
MhlI GDGCHC 2 cut(s) 1345, 1518
Mly113I GGCGCC 1 cut(s) 909
MlyI GAGTC 6 cut(s) 209, 240, 797, 897, 1411, 1580
MmeI TCCRAC 3 cut(s) 134, 237, 1267
MseI TTAA 5 cut(s) 267, 438, 1032, 1113, 1707
MslI CAYNNNNRTG 2 cut(s) 1335, 1754
MspA1I CMGCKG 2 cut(s) 80, 1702
MspI CCGG 1 cut(s) 612
MspR9I CCNGG 1 cut(s) 392
MunI CAATTG 2 cut(s) 683, 1303
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 2 cut(s) 32, 1763
MwoI GCNNNNNNNGC 5 cut(s) 561, 959, 962, 1805, 1871
NarI GGCGCC 1 cut(s) 909
NcoI CCATGG 1 cut(s) 132
NdeI CATATG 1 cut(s) 934
NlaIII CATG 7 cut(s) 136, 244, 451, 908, 1131, 1156, 1178
NlaIV GGNNCC 3 cut(s) 396, 810, 910
NmuCI GTSAC 2 cut(s) 1414, 1627
NruI TCGCGA 1 cut(s) 1763
NspI RCATGY 1 cut(s) 1156
NspV TTCGAA 1 cut(s) 1280
PaeR7I CTCGAG 1 cut(s) 1718
PaqCI CACCTGC 1 cut(s) 1360
PciI ACATGT 1 cut(s) 1152
PcsI WCGNNNNNNNCGW 2 cut(s) 1575, 1782
PfeI GAWTC 6 cut(s) 332, 760, 1290, 1373, 1549, 1770
PinAI ACCGGT 1 cut(s) 611
PkrI GCNGC 3 cut(s) 955, 964, 1057
PleI GAGTC 6 cut(s) 209, 240, 797, 896, 1411, 1579
PluTI GGCGCC 1 cut(s) 912
PpsI GAGTC 6 cut(s) 209, 240, 797, 896, 1411, 1579
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 394
PscI ACATGT 1 cut(s) 1152
PshBI ATTAAT 1 cut(s) 267
Psp124BI GAGCTC 1 cut(s) 1518
Psp5II RGGWCCY 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 390
PspFI CCCAGC 1 cut(s) 860
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 3 cut(s) 396, 810, 910
PspPI GGNCC 3 cut(s) 394, 808, 1016
PspPPI RGGWCCY 1 cut(s) 394
PspXI VCTCGAGB 1 cut(s) 1718
PsuI RGATCY 4 cut(s) 176, 538, 664, 1489
PvuII CAGCTG 2 cut(s) 80, 1702
RruI TCGCGA 1 cut(s) 1763
RsaI GTAC 4 cut(s) 301, 343, 915, 928
RsaNI GTAC 4 cut(s) 300, 342, 914, 927
RseI CAYNNNNRTG 2 cut(s) 1335, 1754
SacI GAGCTC 1 cut(s) 1518
SaqAI TTAA 5 cut(s) 267, 438, 1032, 1113, 1707
SatI GCNGC 3 cut(s) 954, 963, 1056
Sau96I GGNCC 3 cut(s) 394, 808, 1016
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 6 cut(s) 209, 240, 797, 897, 1411, 1580
ScrFI CCNGG 1 cut(s) 392
SduI GDGCHC 2 cut(s) 1345, 1518
SfcI CTRYAG 1 cut(s) 1866
SfoI GGCGCC 1 cut(s) 910
Sfr274I CTCGAG 1 cut(s) 1718
SfuI TTCGAA 1 cut(s) 1280
SinI GGWCC 3 cut(s) 394, 808, 1016
SlaI CTCGAG 1 cut(s) 1718
SmiMI CAYNNNNRTG 2 cut(s) 1335, 1754
SmlI CTYRAG 2 cut(s) 1419, 1718
SmoI CTYRAG 2 cut(s) 1419, 1718
SsiI CCGC 3 cut(s) 114, 768, 962
SspDI GGCGCC 1 cut(s) 908
SspMI CTAG 3 cut(s) 117, 420, 917
SstI GAGCTC 1 cut(s) 1518
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 3 cut(s) 132, 1167, 1509
TaaI ACNGT 6 cut(s) 346, 604, 923, 1125, 1187, 1456
TaiI ACGT 4 cut(s) 38, 130, 634, 856
TaqII GACCGA 1 cut(s) 1087
TatI WGTACW 1 cut(s) 341
TauI GCSGC 1 cut(s) 965
TfiI GAWTC 6 cut(s) 332, 760, 1290, 1373, 1549, 1770
Tru1I TTAA 5 cut(s) 267, 438, 1032, 1113, 1707
Tru9I TTAA 5 cut(s) 267, 438, 1032, 1113, 1707
TscAI CASTG 2 cut(s) 12, 928
TseFI GTSAC 2 cut(s) 1414, 1627
TseI GCWGC 2 cut(s) 953, 1055
Tsp45I GTSAC 2 cut(s) 1414, 1627
TspDTI ATGAA 6 cut(s) 537, 573, 869, 1319, 1383, 1875
TspGWI ACGGA 4 cut(s) 815, 1221, 1750, 1840
TspRI CASTG 2 cut(s) 12, 928
VneI GTGCAC 1 cut(s) 1341
VpaK11BI GGWCC 3 cut(s) 394, 808, 1016
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 4 cut(s) 163, 401, 1027, 1276
XceI RCATGY 1 cut(s) 1156
XhoI CTCGAG 1 cut(s) 1718
XspI CTAG 3 cut(s) 117, 420, 917
ZraI GACGTC 1 cut(s) 128
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.