Rw2G015140

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008276
Physical Location & Seq
Forward (+)
17477316 .. 17481463
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015140.1

Sequence Viewer

Length: 1845 bp
ATGGCCGAGGAGGCGTCACCGACTCGCCTCGCCTTCATCCTCATTGTTCTCGTGATCTTCATTATCGATTCCGTCCATGCCTTTGCCAATCCGAGCACCCCTGCTCTCCTTCTCCATTCTCTTCCAGTCCCGGCCATGGTGTTACCTCTCTACCACTCCACTCCCAATTCCTCTTCTTCTTCTTCGACGTACTATACGACCCGGCTTTGGATCGGAACGCCGCCGCAGATGTTTGCTCTGATTGTCGATACCGGCAGCACTGTCACCTATGTGCCTTGCTCCTCTTGTGAACGGTGTGGCAGACACCAGGACCCTAAGTTTGATCCCGAATCGTCGAACACGTACCAAGCTGTGAAATGCAACATAGATTGTTCCTGTGACAATGACAGGGTGAACTGTATATACGAGAGGCAGTATGCCGAAAGGAGCAGTAGTAGTGGTGTGCTGGGCGAGGATCTTATATCATTTGGGAACCGGAGTGATCTCAAGCCTCAGAGAGCGGTTTTCGGGTGTGAGAATGTGGAGACCGGTGACCTGTTCAGCCAGGCTGCCGATGGGATAATGGGATTGGGACGTGGTGATCTAAGTGTGGTGGATCAGCTTGTTGGGAAAGGTGTTATAAGTGATTCGTTCTCCTTGTGTTATGGTGGGATGGAAATTGGCGGGGGTGCAATGGTTCTTGGTGGCATCGCAGCTCCCGAAGACATGGTCTTTACGCAATCAAACGCCGGACGAAGTCCATACTACAATATTGATTTGAAGGAGATACATGTAGCTGGGAAGCAATTGGCTCTGAATCCAAGCGTCTTTGATGGAAAACATGGAACAGTCCTGGACAGTGGCACAACATACGCTTACCTTCCAGAAGCAGCATTTCAAGCATTTAAAGAGGCCATCATGAAGGAAGTTAATTCACTCCAACAGATCCGTGGCCCTGATCCCAACTATAATGATATCTGTTTTTCCACTGATGTAAGTGATGTGTCCCAACTTTCAAGCACCTTTCCACAAGTTGATATGATATTTGGCAATGGGAAAAAGTTTGGCCTATCTCCTGAAAATTACTTATTCCGACATTCAAAGGTGCATGGTGCTTATTGTCTTGGGATTTTCCAAAATGGAAAGGATCCAACTACACTTTTGGGAGGAATCCTTGTCCGCAATACTCTTGTAACATACGACCGTGAGCATTCCAAGATTGGTTTTTGGAAAACTAATTGTTCTGAGTTGTGGGAAAGACTTCATGTATCTCCTTCACCTTTACCAATGCCTCCTACTTCTGAGGAAAAGAATCCAACTGCCAAAATACAGCCTACAGTAGCTCCATCTGGATCGCCATATGTACTTTCAGGAGACCTCCAAATTGGGCACATAACATTTGATATGTCTTTGAACATCAGCTACTCAGATCTGAAGCCCCACATTACAGAACTTGGTGAATTCATAGCTCAGGAATTAGACATTAACACTTCACAGGTCAATTTGGTGAAATTTTCTTCCAGTGGAAATCATTCCTTTATTAAATGGGACATAACCCCTGCAGATTCAACCGAATACATTTCTAATACAACAGCTGTGGCTATAATTTCCAGGCTTGCTGAACATCGGTTGCAGTTTCCTGTCATGTTTGGAAGTTATCAGTTGATTGATTGGAGGGTTGAGCCCAAGGCAAAACGGACGTGGTGGAAGAATAGTCATGTGGTGGTTATATCAATTTTAGTCGTTCTTGTTATTGGATTATTAGTTTCTGGATTGTGGCTTTTTTGGAGATATCGACAACGGACCGTCAATCCATACAAGCCTGTGGATGCGACTGTTACAGAGGCAGAACTCCAGCCACTTTGA

Protein Analysis

614

Amino Acids

67.58

Weight (kDa)

5.48

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 65 - 229 1.6e-35 Xylanase inhibitor N-terminal
Asp PF00026 65 - 402 1.1e-34 Eukaryotic aspartyl protease
TAXi_C PF14541 249 - 402 1.7e-29 Xylanase inhibitor C-terminal
ALE2_N PF23180 457 - 550 5.5e-06 Receptor-like PK ALE2 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 620
AccBSI CCGCTC 1 cut(s) 500
AciI CCGC 5 cut(s) 221, 224, 500, 663, 1159
AclWI GGATC 9 cut(s) 218, 317, 462, 603, 919, 932, 1121, 1134, 1339
AcoI YGGCCR 2 cut(s) 3, 132
AcsI RAATTY 2 cut(s) 1439, 1490
AcuI CTGAAG 1 cut(s) 1433
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 3 cut(s) 191, 344, 1344
AfiI CCNNNNNNNGG 2 cut(s) 136, 207
AflIII ACRYGT 2 cut(s) 339, 769
AgeI ACCGGT 1 cut(s) 527
AgsI TTSAA 6 cut(s) 760, 878, 996, 1080, 1393, 1548
AjiI CACGTC 2 cut(s) 575, 1680
AjnI CCWGG 4 cut(s) 306, 543, 831, 1589
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 8 cut(s) 350, 601, 695, 776, 1322, 1401, 1448, 1574
AluI AGCT 8 cut(s) 350, 601, 695, 776, 1322, 1401, 1448, 1574
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 2 cut(s) 518, 1347
AlwI GGATC 9 cut(s) 218, 317, 462, 603, 919, 932, 1121, 1134, 1339
AoxI GGCC 5 cut(s) 3, 132, 891, 931, 1045
ApeKI GCWGC 4 cut(s) 255, 548, 692, 869
ApoI RAATTY 2 cut(s) 1439, 1490
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 693, 725
AsiGI ACCGGT 1 cut(s) 527
Asp700I GAANNNNTTC 2 cut(s) 1239, 1510
AspS9I GGNCC 3 cut(s) 310, 932, 1782
AsuC2I CCSGG 2 cut(s) 131, 202
AsuHPI GGTGA 8 cut(s) 9, 256, 403, 542, 590, 1248, 1448, 1498
AvaII GGWCC 2 cut(s) 310, 1782
BaeGI GKGCMC 1 cut(s) 1371
BamHI GGATCC 1 cut(s) 1126
BanII GRGCYC 1 cut(s) 1665
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 708
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 4 cut(s) 267, 535, 704, 881
BccI CCATC 5 cut(s) 548, 646, 806, 902, 1333
BciT130I CCWGG 4 cut(s) 308, 545, 833, 1591
BcnI CCSGG 2 cut(s) 131, 202
BcoDI GTCTC 2 cut(s) 518, 1347
BfmI CTRYAG 2 cut(s) 1314, 1539
BglI GCCNNNNNGGC 1 cut(s) 11
BglII AGATCT 1 cut(s) 1408
BisI GCNGC 6 cut(s) 221, 224, 256, 549, 693, 870
BlsI GCNGC 6 cut(s) 222, 225, 257, 550, 694, 871
Bme1390I CCNGG 6 cut(s) 131, 202, 308, 545, 833, 1591
Bme18I GGWCC 2 cut(s) 310, 1782
BmgBI CACGTC 2 cut(s) 575, 1680
BmgT120I GGNCC 3 cut(s) 310, 932, 1782
BmiI GGNNCC 3 cut(s) 312, 473, 1128
BmrFI CCNGG 6 cut(s) 131, 202, 308, 545, 833, 1591
BmsI GCATC 2 cut(s) 696, 1798
BpiI GAAGAC 1 cut(s) 708
BpmI CTGGAG 1 cut(s) 1817
Bpu10I CCTNAGC 1 cut(s) 1449
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 2 cut(s) 131, 202
Bsa29I ATCGAT 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 2 cut(s) 518, 1347
BsaJI CCNNGG 4 cut(s) 6, 135, 928, 1665
BsaWI WCCGGW 2 cut(s) 474, 527
BsaXI ACNNNNNCTCC 4 cut(s) 418, 448, 1306, 1336
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 207
Bse118I RCCGGY 2 cut(s) 251, 527
Bse1I ACTGG 2 cut(s) 125, 1500
Bse3DI GCAATG 2 cut(s) 678, 1036
BseBI CCWGG 4 cut(s) 308, 545, 833, 1591
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 4 cut(s) 6, 135, 928, 1665
BseGI GGATG 3 cut(s) 36, 657, 1813
BseLI CCNNNNNNNGG 2 cut(s) 136, 207
BseMI GCAATG 2 cut(s) 678, 1036
BseMII CTCAG 5 cut(s) 506, 1215, 1272, 1419, 1463
BseNI ACTGG 2 cut(s) 125, 1500
BseRI GAGGAG 2 cut(s) 23, 271
BseSI GKGCMC 1 cut(s) 1371
BseXI GCAGC 4 cut(s) 267, 535, 704, 881
BseYI CCCAGC 2 cut(s) 445, 776
Bsh1285I CGRYCG 1 cut(s) 1183
BshFI GGCC 5 cut(s) 5, 134, 893, 933, 1047
BshTI ACCGGT 1 cut(s) 527
BshVI ATCGAT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 1183
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 6 cut(s) 131, 202, 252, 475, 528, 729
BslFI GGGAC 4 cut(s) 113, 585, 970, 1541
BslI CCNNNNNNNGG 2 cut(s) 136, 207
BsmAI GTCTC 2 cut(s) 518, 1347
BsmFI GGGAC 4 cut(s) 113, 585, 970, 1541
BsmI GAATGC 1 cut(s) 1189
BsnI GGCC 5 cut(s) 5, 134, 893, 933, 1047
Bso31I GGTCTC 2 cut(s) 518, 1347
Bsp1286I GDGCHC 3 cut(s) 98, 1371, 1665
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 5 cut(s) 221, 224, 500, 663, 1159
BspANI GGCC 5 cut(s) 5, 134, 893, 933, 1047
BspCNI CTCAG 5 cut(s) 505, 1216, 1273, 1418, 1462
BspDI ATCGAT 1 cut(s) 66
BspHI TCATGA 1 cut(s) 897
BspLI GGNNCC 3 cut(s) 312, 473, 1128
BspMAI CTGCAG 1 cut(s) 1543
BspPI GGATC 9 cut(s) 218, 317, 462, 603, 919, 932, 1121, 1134, 1339
BspTNI GGTCTC 2 cut(s) 518, 1347
BsrBI CCGCTC 1 cut(s) 500
BsrDI GCAATG 2 cut(s) 678, 1036
BsrFI RCCGGY 2 cut(s) 251, 527
BsrI ACTGG 2 cut(s) 125, 1500
BssAI RCCGGY 2 cut(s) 251, 527
BssECI CCNNGG 4 cut(s) 6, 135, 928, 1665
BssNI GRCGYC 1 cut(s) 14
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 2 cut(s) 135, 1665
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 4 cut(s) 308, 545, 833, 1591
Bst4CI ACNGT 9 cut(s) 262, 294, 398, 829, 839, 1184, 1318, 1786, 1816
Bst6I CTCTTC 2 cut(s) 126, 178
BstACI GRCGYC 1 cut(s) 14
BstBAI YACGTR 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 1596
BstDEI CTNAG 7 cut(s) 315, 492, 584, 1224, 1281, 1405, 1449
BstDSI CCRYGG 2 cut(s) 135, 928
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 3 cut(s) 36, 657, 1813
BstMAI GTCTC 2 cut(s) 518, 1347
BstMCI CGRYCG 1 cut(s) 1183
BstMWI GCNNNNNNNGC 2 cut(s) 11, 878
BstNI CCWGG 4 cut(s) 308, 545, 833, 1591
BstNSI RCATGY 1 cut(s) 773
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 6 cut(s) 129, 200, 306, 543, 831, 1589
BstSFI CTRYAG 2 cut(s) 1314, 1539
BstSLI GKGCMC 1 cut(s) 1371
BstV1I GCAGC 4 cut(s) 267, 535, 704, 881
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 4 cut(s) 454, 924, 1126, 1408
BstYI RGATCY 4 cut(s) 454, 924, 1126, 1408
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 5 cut(s) 5, 134, 893, 933, 1047
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 2 cut(s) 135, 928
BtgZI GCGATG 1 cut(s) 673
BtrI CACGTC 2 cut(s) 575, 1680
BtsCI GGATG 3 cut(s) 36, 657, 1813
BtsIMutI CAGTG 4 cut(s) 258, 844, 966, 1507
Cac8I GCNNGC 1 cut(s) 1596
CciI TCATGA 1 cut(s) 897
Cfr10I RCCGGY 2 cut(s) 251, 527
Cfr13I GGNCC 3 cut(s) 310, 932, 1782
ClaI ATCGAT 1 cut(s) 66
CpoI CGGWCCG 1 cut(s) 1782
CseI GACGC 2 cut(s) 3, 793
Csp6I GTAC 3 cut(s) 190, 343, 1343
CspAI ACCGGT 1 cut(s) 527
CspCI CAANNNNNGTGG 2 cut(s) 1557, 1592
CspI CGGWCCG 1 cut(s) 1782
CviQI GTAC 3 cut(s) 190, 343, 1343
DdeI CTNAG 7 cut(s) 315, 492, 584, 1224, 1281, 1405, 1449
DraI TTTAAA 1 cut(s) 886
EaeI YGGCCR 2 cut(s) 3, 132
Eam1104I CTCTTC 2 cut(s) 126, 178
EarI CTCTTC 2 cut(s) 126, 178
Eco130I CCWWGG 2 cut(s) 135, 1665
Eco24I GRGCYC 1 cut(s) 1665
Eco31I GGTCTC 2 cut(s) 518, 1347
Eco32I GATATC 2 cut(s) 955, 1772
Eco47I GGWCC 2 cut(s) 310, 1782
Eco57I CTGAAG 1 cut(s) 1433
Eco91I GGTNACC 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 310
EcoO65I GGTNACC 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 1439
EcoRII CCWGG 4 cut(s) 306, 543, 831, 1589
EcoRV GATATC 2 cut(s) 955, 1772
EcoT14I CCWWGG 2 cut(s) 135, 1665
EcoT38I GRGCYC 1 cut(s) 1665
ErhI CCWWGG 2 cut(s) 135, 1665
FaqI GGGAC 4 cut(s) 113, 585, 970, 1541
FauI CCCGC 1 cut(s) 656
FauNDI CATATG 1 cut(s) 1339
Fnu4HI GCNGC 6 cut(s) 221, 224, 256, 549, 693, 870
FokI GGATG 3 cut(s) 23, 664, 1820
FriOI GRGCYC 1 cut(s) 1665
Fsp4HI GCNGC 6 cut(s) 221, 224, 256, 549, 693, 870
GluI GCNGC 6 cut(s) 221, 224, 256, 549, 693, 870
GsaI CCCAGC 2 cut(s) 449, 780
GsuI CTGGAG 1 cut(s) 1817
HaeIII GGCC 5 cut(s) 5, 134, 893, 933, 1047
HapII CCGG 6 cut(s) 131, 202, 252, 475, 528, 729
HgaI GACGC 2 cut(s) 3, 793
Hin1I GRCGYC 1 cut(s) 14
HinfI GANTC 8 cut(s) 22, 68, 329, 626, 796, 1149, 1291, 1544
HpaII CCGG 6 cut(s) 131, 202, 252, 475, 528, 729
HphI GGTGA 8 cut(s) 9, 256, 403, 542, 590, 1248, 1448, 1498
Hpy166II GTNNAC 2 cut(s) 290, 394
Hpy8I GTNNAC 2 cut(s) 290, 394
Hpy99I CGWCG 2 cut(s) 190, 337
HpyAV CCTTC 6 cut(s) 43, 119, 754, 869, 895, 1263
HpyCH4III ACNGT 9 cut(s) 262, 294, 398, 829, 839, 1184, 1318, 1786, 1816
HpyCH4IV ACGT 4 cut(s) 188, 341, 574, 1679
HpyCH4V TGCA 5 cut(s) 360, 671, 1087, 1541, 1612
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 878
HpyF3I CTNAG 7 cut(s) 315, 492, 584, 1224, 1281, 1405, 1449
HpySE526I ACGT 4 cut(s) 188, 341, 574, 1679
Hsp92I GRCGYC 1 cut(s) 14
LmnI GCTCC 4 cut(s) 284, 426, 700, 1327
Lsp1109I GCAGC 4 cut(s) 267, 535, 704, 881
LweI GCATC 2 cut(s) 696, 1798
MaeII ACGT 4 cut(s) 188, 341, 574, 1679
MaeIII GTNAC 7 cut(s) 15, 141, 262, 377, 530, 1171, 1816
MbiI CCGCTC 1 cut(s) 500
MboII GAAGA 9 cut(s) 49, 113, 165, 168, 171, 174, 713, 1488, 1699
MfeI CAATTG 1 cut(s) 785
MflI RGATCY 4 cut(s) 454, 924, 1126, 1408
MhlI GDGCHC 3 cut(s) 98, 1371, 1665
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 4 cut(s) 943, 1096, 1154, 1319
MroXI GAANNNNTTC 2 cut(s) 1239, 1510
MseI TTAA 4 cut(s) 885, 909, 1464, 1521
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 1574
MspI CCGG 6 cut(s) 131, 202, 252, 475, 528, 729
MspR9I CCNGG 6 cut(s) 131, 202, 308, 545, 833, 1591
MunI CAATTG 1 cut(s) 785
Mva1269I GAATGC 1 cut(s) 1189
MvaI CCWGG 4 cut(s) 308, 545, 833, 1591
MwoI GCNNNNNNNGC 2 cut(s) 11, 878
NciI CCSGG 2 cut(s) 131, 202
NcoI CCATGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 1339
NlaIV GGNNCC 3 cut(s) 312, 473, 1128
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 4 cut(s) 15, 262, 377, 530
NspI RCATGY 1 cut(s) 773
OliI CACNNNNGTG 1 cut(s) 269
PagI TCATGA 1 cut(s) 897
PciI ACATGT 1 cut(s) 769
PcsI WCGNNNNNNNCGW 3 cut(s) 194, 338, 696
PctI GAATGC 1 cut(s) 1189
PdmI GAANNNNTTC 2 cut(s) 1239, 1510
PfeI GAWTC 7 cut(s) 68, 329, 626, 796, 1149, 1291, 1544
PflFI GACNNNGTC 2 cut(s) 707, 735
PfoI TCCNGGA 1 cut(s) 831
PinAI ACCGGT 1 cut(s) 527
PkrI GCNGC 6 cut(s) 222, 225, 257, 550, 694, 871
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 1 cut(s) 342
PpuMI RGGWCCY 1 cut(s) 310
PscI ACATGT 1 cut(s) 769
PsiI TTATAA 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 4 cut(s) 306, 543, 831, 1589
PspEI GGTNACC 1 cut(s) 530
PspFI CCCAGC 2 cut(s) 445, 776
PspGI CCWGG 4 cut(s) 306, 543, 831, 1589
PspN4I GGNNCC 3 cut(s) 312, 473, 1128
PspPI GGNCC 3 cut(s) 310, 932, 1782
PspPPI RGGWCCY 1 cut(s) 310
PsrI GAACNNNNNNTAC 4 cut(s) 386, 418, 1385, 1417
PstI CTGCAG 1 cut(s) 1543
PsuI RGATCY 4 cut(s) 454, 924, 1126, 1408
PsyI GACNNNGTC 2 cut(s) 707, 735
PvuII CAGCTG 1 cut(s) 1574
RsaI GTAC 3 cut(s) 191, 344, 1344
RsaNI GTAC 3 cut(s) 190, 343, 1343
RseI CAYNNNNRTG 1 cut(s) 269
Rsr2I CGGWCCG 1 cut(s) 1782
RsrII CGGWCCG 1 cut(s) 1782
SaqAI TTAA 4 cut(s) 885, 909, 1464, 1521
SatI GCNGC 6 cut(s) 221, 224, 256, 549, 693, 870
Sau96I GGNCC 3 cut(s) 310, 932, 1782
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 6 cut(s) 131, 202, 308, 545, 833, 1591
SduI GDGCHC 3 cut(s) 98, 1371, 1665
SfaNI GCATC 2 cut(s) 696, 1798
SfcI CTRYAG 2 cut(s) 1314, 1539
SinI GGWCC 2 cut(s) 310, 1782
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
SsiI CCGC 5 cut(s) 221, 224, 500, 663, 1159
SspI AATATT 1 cut(s) 751
StyD4I CCNGG 6 cut(s) 129, 200, 306, 543, 831, 1589
StyI CCWWGG 2 cut(s) 135, 1665
TaaI ACNGT 9 cut(s) 262, 294, 398, 829, 839, 1184, 1318, 1786, 1816
TaiI ACGT 4 cut(s) 191, 344, 577, 1682
TaqI TCGA 5 cut(s) 66, 185, 246, 335, 1774
TatI WGTACW 1 cut(s) 1342
TauI GCSGC 2 cut(s) 223, 226
TfiI GAWTC 7 cut(s) 68, 329, 626, 796, 1149, 1291, 1544
Tru1I TTAA 4 cut(s) 885, 909, 1464, 1521
Tru9I TTAA 4 cut(s) 885, 909, 1464, 1521
TscAI CASTG 4 cut(s) 265, 844, 973, 1507
TseFI GTSAC 4 cut(s) 15, 262, 377, 530
TseI GCWGC 4 cut(s) 255, 548, 692, 869
Tsp45I GTSAC 4 cut(s) 15, 262, 377, 530
TspDTI ATGAA 5 cut(s) 25, 49, 914, 1232, 1432
TspGWI ACGGA 4 cut(s) 61, 917, 1690, 1795
TspRI CASTG 4 cut(s) 265, 844, 973, 1507
Tth111I GACNNNGTC 2 cut(s) 707, 735
VpaK11BI GGWCC 2 cut(s) 310, 1782
XapI RAATTY 2 cut(s) 1439, 1490
XceI RCATGY 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 2 cut(s) 551, 926
XmnI GAANNNNTTC 2 cut(s) 1239, 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.