Rorug02G0140800

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008276
Physical Location & Seq
Reverse (-)
12394614 .. 12396540
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0140800.1

Sequence Viewer

Length: 1161 bp
ATGGCGGATTACTTACCCGAAGACGTGATAGTTCGGATCCTGGAGAGGTTGCCGATCAAATCCCTAATCCGCTTCACCTGCGTTTCGAAACACTGGCGTTTCATCATATTGTCCGACCCAGAATTCGCCAAGACCCAATTCCAAATAGCCTCTCAACACCAAACCCCCCGTCACAGGCTCCTCATCTCCACCGCCTGTGTATCTGAATTCAACTCGCTAGACTTGGATGCGCCGCCGTCGCCGTCGCCGTTGGGATACAATTCTTCGGTTAGGTGCCCATTCACGCAACCGGGGGGCCGTCATGTCATACTACTGTGCTCCTGCAATGGTTTGATACTTGCAACTCTTCATAAAGAAGAAAACATGTATATCTGGAACCCATCAACTCAGTTCTTCAAAAAATTACCTGACCCAGCTGATCGTCCCGCAAATACTCGTCTATTCGGCTGTGGTTTTGGCTATTTGTCGGCCACTGACGACTACAAAGTTGTTGCGGACTTCAGAGTCAGTTCTGTTGATGAGGTGAAGGGCAAGGAGATGCACATCTTCTCATCAAAAGCCAACATTTGGAAAAGGACTGAAGCCCCCCCTCTCTTCGACTACGAGTTTGAATATGAGGGGACTCTTTCAAATGAGGCACTTCATTGGCTGGATGAACTTGGCATCGTTGCTTTTGATCTAGCAAAGGAGGAGTTCCAAAGAATGCCGCTGCCTATTCAAGATGGTGAGGTTCAGTCTCTCGGGGCTTTCGGAGACTGTCTGTGTGCATTTGATTGTGCAAAACTTGATACTGCTGGATCCATTGATTTGTGGGTGATGAAAAAATATGGCGTGGCTGACTCATGGACGAAGCTCTTTAACTTAAAGGTTTCCGATCAGCCCGATCAGCCTGAGAATATAATGTGCTGTTTGCCGGTCTCTGTAATGGAAACTAGTACATTTATGCAGAAAGTGACTAAACCCTTTGGTGGGAAACAGAAGTTTCTTAATTTGATCAGGATTGGTCATACGAAACAGAAGCCCGAAATGCATGAGTTGATCAACAAATCAGAATACAAGGAAATGGTCGAATATGAAGAGACTCTACTTTGTCTTGCGGCCCCTAAAATAGAAGGAAGGCAAGGGCAAGTATGTGGACCAAATGAAGTGGATGCATCTTGA

Protein Analysis

386

Amino Acids

43.65

Weight (kDa)

5.48

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 3 - 39 9.5e-08 F-box-like
F-box PF00646 5 - 40 4.8e-10 F-box domain
FBA_3 PF08268 89 - 284 1e-11 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 93 - 241 6.7e-07 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 105 - 315 1.6e-21 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
AasI GACNNNNNNGTC 1 cut(s) 503
Acc36I ACCTGC 1 cut(s) 86
AccB1I GGYRCC 1 cut(s) 273
AccB7I CCANNNNNTGG 1 cut(s) 567
AciI CCGC 8 cut(s) 5, 70, 192, 233, 426, 494, 707, 1097
AclWI GGATC 4 cut(s) 31, 44, 792, 805
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 2 cut(s) 122, 206
AcuI CTGAAG 2 cut(s) 484, 600
AfaI GTAC 1 cut(s) 937
AfiI CCNNNNNNNGG 4 cut(s) 174, 567, 968, 969
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 5 cut(s) 211, 397, 611, 630, 719
AhlI ACTAGT 1 cut(s) 932
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 39
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 2 cut(s) 416, 853
AluI AGCT 2 cut(s) 416, 853
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 4 cut(s) 741, 747, 922, 1073
AlwI GGATC 4 cut(s) 31, 44, 792, 805
Ama87I CYCGRG 1 cut(s) 740
AoxI GGCC 3 cut(s) 295, 468, 1098
ApeKI GCWGC 1 cut(s) 709
ApoI RAATTY 2 cut(s) 122, 206
ArsI GACNNNNNNTTYG 6 cut(s) 107, 139, 154, 186, 590, 622
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 3 cut(s) 295, 1099, 1136
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 4 cut(s) 67, 535, 737, 826
AsuII TTCGAA 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 740
AvaII GGWCC 1 cut(s) 1136
BaeGI GKGCMC 1 cut(s) 278
BamHI GGATCC 2 cut(s) 36, 797
BanI GGYRCC 1 cut(s) 273
BarI GAAGNNNNNNTAC 2 cut(s) 1068, 1100
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 696
BccI CCATC 2 cut(s) 388, 716
BceAI ACGGC 4 cut(s) 220, 226, 232, 282
BciT130I CCWGG 1 cut(s) 41
BciVI GTATCC 1 cut(s) 248
BclI TGATCA 2 cut(s) 993, 1038
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 4 cut(s) 741, 747, 922, 1073
BcuI ACTAGT 1 cut(s) 932
BfaI CTAG 3 cut(s) 218, 680, 933
BfuAI ACCTGC 1 cut(s) 86
BfuI GTATCC 1 cut(s) 248
BisI GCNGC 4 cut(s) 233, 707, 710, 1098
BlsI GCNGC 4 cut(s) 234, 708, 711, 1099
Bme1390I CCNGG 2 cut(s) 41, 291
Bme18I GGWCC 1 cut(s) 1136
BmeT110I CYCGRG 1 cut(s) 740
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 295, 1099, 1136
BmiI GGNNCC 7 cut(s) 38, 179, 275, 296, 377, 799, 1101
BmrFI CCNGG 2 cut(s) 41, 291
BmsI GCATC 4 cut(s) 217, 528, 672, 1141
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 542
BsaI GGTCTC 1 cut(s) 922
BsaJI CCNNGG 1 cut(s) 290
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 567, 968, 969
Bse118I RCCGGY 1 cut(s) 913
Bse1I ACTGG 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 542
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 3 cut(s) 232, 658, 1156
BseJI GATNNNNATC 1 cut(s) 542
BseLI CCNNNNNNNGG 4 cut(s) 174, 567, 968, 969
BseMI GCAATG 1 cut(s) 331
BseMII CTCAG 2 cut(s) 401, 882
BseNI ACTGG 1 cut(s) 98
BseRI GAGGAG 2 cut(s) 170, 704
BseSI GKGCMC 1 cut(s) 278
BseXI GCAGC 1 cut(s) 696
BseYI CCCAGC 1 cut(s) 412
BshFI GGCC 3 cut(s) 297, 470, 1100
BshNI GGYRCC 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 1 cut(s) 740
BsiSI CCGG 2 cut(s) 290, 914
BslFI GGGAC 2 cut(s) 408, 634
BslI CCNNNNNNNGG 4 cut(s) 174, 567, 968, 969
BsmAI GTCTC 4 cut(s) 741, 747, 922, 1073
BsmFI GGGAC 2 cut(s) 408, 634
BsmI GAATGC 1 cut(s) 708
BsnI GGCC 3 cut(s) 297, 470, 1100
Bso31I GGTCTC 1 cut(s) 922
BsoBI CYCGRG 1 cut(s) 740
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 278, 320
Bsp143I GATC 9 cut(s) 36, 54, 418, 676, 797, 874, 883, 993, 1038
BspACI CCGC 8 cut(s) 5, 70, 192, 233, 426, 494, 707, 1097
BspANI GGCC 3 cut(s) 297, 470, 1100
BspCNI CTCAG 2 cut(s) 400, 883
BspLI GGNNCC 7 cut(s) 38, 179, 275, 296, 377, 799, 1101
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 4 cut(s) 31, 44, 792, 805
BspT104I TTCGAA 1 cut(s) 86
BspT107I GGYRCC 1 cut(s) 273
BspTNI GGTCTC 1 cut(s) 922
BsrDI GCAATG 1 cut(s) 331
BsrFI RCCGGY 1 cut(s) 913
BsrI ACTGG 1 cut(s) 98
BssAI RCCGGY 1 cut(s) 913
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 9 cut(s) 36, 54, 418, 676, 797, 874, 883, 993, 1038
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 315, 758
Bst6I CTCTTC 3 cut(s) 351, 599, 1071
BstBI TTCGAA 1 cut(s) 86
BstDEI CTNAG 2 cut(s) 387, 891
BstF5I GGATG 3 cut(s) 232, 658, 1156
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 9 cut(s) 39, 57, 421, 679, 800, 877, 886, 996, 1041
BstMAI GTCTC 4 cut(s) 741, 747, 922, 1073
BstMBI GATC 9 cut(s) 36, 54, 418, 676, 797, 874, 883, 993, 1038
BstMWI GCNNNNNNNGC 4 cut(s) 78, 238, 886, 1027
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 2 cut(s) 39, 289
BstSLI GKGCMC 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 696
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 2 cut(s) 36, 797
BstYI RGATCY 2 cut(s) 36, 797
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 3 cut(s) 297, 470, 1100
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 3 cut(s) 232, 658, 1156
BtsIMutI CAGTG 2 cut(s) 91, 471
BveI ACCTGC 1 cut(s) 86
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 913
Cfr13I GGNCC 3 cut(s) 295, 1099, 1136
Csp6I GTAC 1 cut(s) 936
CspCI CAANNNNNGTGG 1 cut(s) 1128
CviAII CATG 4 cut(s) 302, 364, 843, 1031
CviQI GTAC 1 cut(s) 936
DdeI CTNAG 2 cut(s) 387, 891
DpnI GATC 9 cut(s) 38, 56, 420, 678, 799, 876, 885, 995, 1040
DpnII GATC 9 cut(s) 36, 54, 418, 676, 797, 874, 883, 993, 1038
DrdI GACNNNNNNGTC 1 cut(s) 503
DseDI GACNNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 3 cut(s) 351, 599, 1071
EarI CTCTTC 3 cut(s) 351, 599, 1071
EciI GGCGGA 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 922
Eco47I GGWCC 1 cut(s) 1136
Eco57I CTGAAG 2 cut(s) 484, 600
Eco88I CYCGRG 1 cut(s) 740
EcoRI GAATTC 2 cut(s) 122, 206
EcoRII CCWGG 1 cut(s) 39
EcoT22I ATGCAT 2 cut(s) 1032, 1156
FaeI CATG 4 cut(s) 305, 367, 846, 1034
FaqI GGGAC 2 cut(s) 408, 634
FatI CATG 4 cut(s) 301, 363, 842, 1030
FauI CCCGC 1 cut(s) 433
FbaI TGATCA 2 cut(s) 993, 1038
Fnu4HI GCNGC 4 cut(s) 233, 707, 710, 1098
FokI GGATG 2 cut(s) 239, 665
Fsp4HI GCNGC 4 cut(s) 233, 707, 710, 1098
FspBI CTAG 3 cut(s) 218, 680, 933
GlaI GCGC 1 cut(s) 231
GluI GCNGC 4 cut(s) 233, 707, 710, 1098
GsaI CCCAGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 297, 470, 1100
HapII CCGG 2 cut(s) 290, 914
HhaI GCGC 1 cut(s) 232
Hin1II CATG 4 cut(s) 305, 367, 846, 1034
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 4 cut(s) 504, 622, 839, 1081
HpaII CCGG 2 cut(s) 290, 914
HphI GGTGA 4 cut(s) 67, 535, 737, 826
Hpy166II GTNNAC 1 cut(s) 1136
Hpy188I TCNGA 7 cut(s) 36, 115, 205, 503, 752, 874, 1051
Hpy188III TCNNGA 4 cut(s) 373, 719, 997, 1158
Hpy8I GTNNAC 1 cut(s) 1136
Hpy99I CGWCG 2 cut(s) 241, 247
HpyAV CCTTC 3 cut(s) 520, 1106, 1110
HpyCH4III ACNGT 2 cut(s) 315, 758
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 8 cut(s) 324, 341, 541, 767, 779, 946, 1030, 1154
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 238, 886, 1027
HpyF3I CTNAG 2 cut(s) 387, 891
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 4 cut(s) 305, 367, 846, 1034
HspAI GCGC 1 cut(s) 230
Ksp22I TGATCA 2 cut(s) 993, 1038
Kzo9I GATC 9 cut(s) 36, 54, 418, 676, 797, 874, 883, 993, 1038
LmnI GCTCC 2 cut(s) 183, 323
Lsp1109I GCAGC 1 cut(s) 696
LweI GCATC 4 cut(s) 217, 528, 672, 1141
MaeI CTAG 3 cut(s) 218, 680, 933
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 170, 952
MalI GATC 9 cut(s) 38, 56, 420, 678, 799, 876, 885, 995, 1040
MboI GATC 9 cut(s) 36, 54, 418, 676, 797, 874, 883, 993, 1038
MboII GAAGA 8 cut(s) 32, 255, 338, 368, 385, 538, 586, 1088
MflI RGATCY 2 cut(s) 36, 797
MhlI GDGCHC 2 cut(s) 278, 320
MluCI AATT 6 cut(s) 122, 137, 206, 259, 401, 988
MlyI GAGTC 4 cut(s) 513, 616, 833, 1075
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 9 cut(s) 39, 160, 191, 514, 600, 610, 628, 682, 721
Mph1103I ATGCAT 2 cut(s) 1032, 1156
MseI TTAA 3 cut(s) 858, 863, 987
MspA1I CMGCKG 2 cut(s) 416, 709
MspI CCGG 2 cut(s) 290, 914
MspR9I CCNGG 2 cut(s) 41, 291
Mva1269I GAATGC 1 cut(s) 708
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 4 cut(s) 78, 238, 886, 1027
NciI CCSGG 1 cut(s) 291
NdeII GATC 9 cut(s) 36, 54, 418, 676, 797, 874, 883, 993, 1038
NlaIII CATG 4 cut(s) 305, 367, 846, 1034
NlaIV GGNNCC 7 cut(s) 38, 179, 275, 296, 377, 799, 1101
NmuCI GTSAC 2 cut(s) 170, 952
NsiI ATGCAT 2 cut(s) 1032, 1156
NspI RCATGY 1 cut(s) 367
NspV TTCGAA 1 cut(s) 86
PaqCI CACCTGC 1 cut(s) 86
PciI ACATGT 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PctI GAATGC 1 cut(s) 708
PflMI CCANNNNNTGG 1 cut(s) 567
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 4 cut(s) 234, 708, 711, 1099
PleI GAGTC 4 cut(s) 512, 616, 833, 1075
PpsI GAGTC 4 cut(s) 512, 616, 833, 1075
PscI ACATGT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 7 cut(s) 38, 179, 275, 296, 377, 799, 1101
PspPI GGNCC 3 cut(s) 295, 1099, 1136
PsuI RGATCY 2 cut(s) 36, 797
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 1 cut(s) 937
RsaNI GTAC 1 cut(s) 936
SaqAI TTAA 3 cut(s) 858, 863, 987
SatI GCNGC 4 cut(s) 233, 707, 710, 1098
Sau3AI GATC 9 cut(s) 36, 54, 418, 676, 797, 874, 883, 993, 1038
Sau96I GGNCC 3 cut(s) 295, 1099, 1136
SchI GAGTC 4 cut(s) 513, 616, 833, 1075
ScrFI CCNGG 2 cut(s) 41, 291
SduI GDGCHC 2 cut(s) 278, 320
SfaNI GCATC 4 cut(s) 217, 528, 672, 1141
SfuI TTCGAA 1 cut(s) 86
SinI GGWCC 1 cut(s) 1136
SpeI ACTAGT 1 cut(s) 932
Sse9I AATT 6 cut(s) 122, 137, 206, 259, 401, 988
SsiI CCGC 8 cut(s) 5, 70, 192, 233, 426, 494, 707, 1097
SspMI CTAG 3 cut(s) 218, 680, 933
StyD4I CCNGG 2 cut(s) 39, 289
TaaI ACNGT 2 cut(s) 315, 758
TaiI ACGT 1 cut(s) 27
TaqI TCGA 3 cut(s) 86, 597, 1068
TasI AATT 6 cut(s) 122, 137, 206, 259, 401, 988
TatI WGTACW 1 cut(s) 935
TauI GCSGC 3 cut(s) 235, 709, 1100
Tru1I TTAA 3 cut(s) 858, 863, 987
Tru9I TTAA 3 cut(s) 858, 863, 987
TscAI CASTG 2 cut(s) 98, 478
TseFI GTSAC 2 cut(s) 170, 952
TseI GCWGC 1 cut(s) 709
Tsp45I GTSAC 2 cut(s) 170, 952
TspDTI ATGAA 7 cut(s) 91, 338, 632, 669, 833, 1089, 1158
TspRI CASTG 2 cut(s) 98, 478
Van91I CCANNNNNTGG 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 1136
XapI RAATTY 2 cut(s) 122, 206
XceI RCATGY 1 cut(s) 367
XspI CTAG 3 cut(s) 218, 680, 933
Zsp2I ATGCAT 2 cut(s) 1032, 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.