AT3G50350

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012887
Physical Location & Seq
Forward (+)
18672102 .. 18674022
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50350.2

Sequence Viewer

Length: 546 bp
ATGAACCAATCAAAAACAAATCCAGCCATTGATCGTAAAACCTTTCCACCACCGCACACCCCCTCGCCGGGGGTAGTGCGTCGCATACACGCGCCACCGTTACCTTCTACTTCTCTCTTGAAGCAACACTCGTGGTCTCCTGATCTAATCCGCGAGGAAGCTTGGTCAAAACGTCAAGATATCTCCCGCCACCGTCATCTCCGCCGTGGTAAGAGCTTGACCGACGAAGATCTCGACGAGCTTAAAGCAAGTTTCGAACTCGGATTCGGTTTCGGATCTCCCGAAAACGCTGATCCAAGACTTTCCAACACGCTTCCCGCGTTAGAACTTTACTTCGCCGTTCAAAAAAGCTACAACGACGCCGTTTCTAACAAATCAACAACGTCGTCGTCTTCACTCTCCGACGGCGATACCAGTCCTCACCACACCGTATACCAAACCAACGAAGCTGAAGCAATGGGCGAGAGTGGTGGCGTGCACCGTGAACCAATCGCCGCCGAGATGATAATTTTAACGACTGTTAAACGTTGTGGGGATAAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

181

Amino Acids

19.97

Weight (kDa)

6.71

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 69 - 127 3.1e-27 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50350 AT3G50350
fragaria_vesca FvH4_1g16050
malus_domestica MD02G1171900.v1.1 MD15G1283800.v1.1
prunus_persica Prupe.7G133400_v2.0.a1
pyrus_communis pycom02g13860 pycom15g24680
rosa_chinensis RchiOBHm_Chr2g0105661
rosa_laevigata RLG00000017447
rosa_multiflora Rmu_sc0001051.1_g000050
rosa_roxburghii Rroxscaffold_2G00138010
rosa_rugosa Rorug02G0129100
rosa_samantha Rh2AG180700 Rh2BG189600 Rh2CG184900 Rh2DG186700
rosa_wichuraiana Rw2G014120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 432
AccII CGCG 3 cut(s) 92, 153, 320
AciI CCGC 6 cut(s) 53, 151, 187, 202, 318, 495
AclI AACGTT 1 cut(s) 526
AclWI GGATC 2 cut(s) 283, 287
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 360
AfiI CCNNNNNNNGG 3 cut(s) 67, 68, 69
AgsI TTSAA 2 cut(s) 121, 344
AluBI AGCT 5 cut(s) 161, 216, 241, 351, 449
AluI AGCT 5 cut(s) 161, 216, 241, 351, 449
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 1 cut(s) 141
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 2 cut(s) 283, 287
ApaLI GTGCAC 1 cut(s) 476
AspLEI GCGC 1 cut(s) 94
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 413
AsuII TTCGAA 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 480
BauI CACGAG 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 480
BceAI ACGGC 4 cut(s) 189, 323, 347, 421
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 141
BglII AGATCT 1 cut(s) 229
BisI GCNGC 1 cut(s) 495
BlsI GCNGC 1 cut(s) 496
Bme1390I CCNGG 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 141
BsaJI CCNNGG 2 cut(s) 68, 205
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 68, 69
Bse1I ACTGG 1 cut(s) 414
Bse3DI GCAATG 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 68, 205
BseLI CCNNNNNNNGG 3 cut(s) 67, 68, 69
BseMI GCAATG 1 cut(s) 462
BseNI ACTGG 1 cut(s) 414
BseSI GKGCMC 1 cut(s) 480
Bsh1236I CGCG 3 cut(s) 92, 153, 320
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 1 cut(s) 68
BslI CCNNNNNNNGG 3 cut(s) 67, 68, 69
BsmAI GTCTC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 141
Bsp119I TTCGAA 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 5 cut(s) 31, 142, 229, 275, 292
BspACI CCGC 6 cut(s) 53, 151, 187, 202, 318, 495
BspFNI CGCG 3 cut(s) 92, 153, 320
BspPI GGATC 2 cut(s) 283, 287
BspT104I TTCGAA 1 cut(s) 255
BspTNI GGTCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 1 cut(s) 414
BssECI CCNNGG 2 cut(s) 68, 205
BssMI GATC 5 cut(s) 31, 142, 229, 275, 292
BssNAI GTATAC 1 cut(s) 433
BssNI GRCGYC 1 cut(s) 360
BssSI CACGAG 1 cut(s) 130
Bst1107I GTATAC 1 cut(s) 433
Bst2BI CACGAG 1 cut(s) 130
Bst4CI ACNGT 5 cut(s) 99, 194, 430, 482, 520
BstACI GRCGYC 1 cut(s) 360
BstBI TTCGAA 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 476
BstDSI CCRYGG 1 cut(s) 205
BstFNI CGCG 3 cut(s) 92, 153, 320
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 5 cut(s) 34, 145, 232, 278, 295
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 5 cut(s) 31, 142, 229, 275, 292
BstSCI CCNGG 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 480
BstUI CGCG 3 cut(s) 92, 153, 320
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 2 cut(s) 229, 275
BstYI RGATCY 2 cut(s) 229, 275
BstZ17I GTATAC 1 cut(s) 433
BtgI CCRYGG 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 476
CfoI GCGC 1 cut(s) 94
CseI GACGC 2 cut(s) 68, 368
CspCI CAANNNNNGTGG 2 cut(s) 113, 148
CviJI RGCY 6 cut(s) 26, 161, 216, 241, 351, 449
CviKI_1 RGCY 6 cut(s) 26, 161, 216, 241, 351, 449
DpnI GATC 5 cut(s) 33, 144, 231, 277, 294
DpnII GATC 5 cut(s) 31, 142, 229, 275, 292
EciI GGCGGA 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 141
Eco32I GATATC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 471
EcoRV GATATC 1 cut(s) 181
FaiI YATR 2 cut(s) 86, 433
FauI CCCGC 2 cut(s) 194, 325
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 1 cut(s) 495
Fsp4HI GCNGC 1 cut(s) 495
GlaI GCGC 1 cut(s) 93
GluI GCNGC 1 cut(s) 495
HapII CCGG 1 cut(s) 68
HgaI GACGC 2 cut(s) 68, 368
HhaI GCGC 1 cut(s) 94
Hin1I GRCGYC 1 cut(s) 360
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 1 cut(s) 264
HpaII CCGG 1 cut(s) 68
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 3 cut(s) 433, 478, 485
Hpy188I TCNGA 3 cut(s) 263, 275, 403
Hpy188III TCNNGA 5 cut(s) 118, 140, 176, 233, 281
Hpy8I GTNNAC 3 cut(s) 433, 478, 485
Hpy99I CGWCG 7 cut(s) 84, 227, 239, 362, 388, 391, 407
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 5 cut(s) 99, 194, 430, 482, 520
HpyCH4IV ACGT 3 cut(s) 172, 383, 526
HpyCH4V TGCA 1 cut(s) 478
HpySE526I ACGT 3 cut(s) 172, 383, 526
Hsp92I GRCGYC 1 cut(s) 360
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 5 cut(s) 31, 142, 229, 275, 292
LpnPI CCDG 4 cut(s) 36, 81, 153, 427
MaeII ACGT 3 cut(s) 172, 383, 526
MaeIII GTNAC 1 cut(s) 99
MalI GATC 5 cut(s) 33, 144, 231, 277, 294
MboI GATC 5 cut(s) 31, 142, 229, 275, 292
MboII GAAGA 2 cut(s) 239, 384
MflI RGATCY 2 cut(s) 229, 275
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 1 cut(s) 507
MmeI TCCRAC 2 cut(s) 330, 426
MnlI CCTC 3 cut(s) 73, 148, 429
MseI TTAA 4 cut(s) 243, 512, 522, 544
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 69
MvnI CGCG 3 cut(s) 92, 153, 320
NciI CCSGG 1 cut(s) 69
NdeII GATC 5 cut(s) 31, 142, 229, 275, 292
NmeAIII GCCGAG 1 cut(s) 523
NspV TTCGAA 1 cut(s) 255
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 279, 317
PfeI GAWTC 1 cut(s) 264
PkrI GCNGC 1 cut(s) 496
Psp1406I AACGTT 1 cut(s) 526
PsuI RGATCY 2 cut(s) 229, 275
SaqAI TTAA 4 cut(s) 243, 512, 522, 544
SatI GCNGC 1 cut(s) 495
Sau3AI GATC 5 cut(s) 31, 142, 229, 275, 292
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 1 cut(s) 480
SfuI TTCGAA 1 cut(s) 255
Sse9I AATT 1 cut(s) 507
SsiI CCGC 6 cut(s) 53, 151, 187, 202, 318, 495
StyD4I CCNGG 1 cut(s) 67
TaaI ACNGT 5 cut(s) 99, 194, 430, 482, 520
TaiI ACGT 3 cut(s) 175, 386, 529
TaqI TCGA 2 cut(s) 234, 255
TaqII GACCGA 1 cut(s) 236
TasI AATT 1 cut(s) 507
TauI GCSGC 1 cut(s) 497
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 4 cut(s) 243, 512, 522, 544
Tru9I TTAA 4 cut(s) 243, 512, 522, 544
TspDTI ATGAA 1 cut(s) 17
VneI GTGCAC 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.