MD02G1171900.v1.1

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012887
Physical Location & Seq
Reverse (-)
14935205 .. 14936419
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1171900.v1.1.491

Sequence Viewer

Length: 471 bp
ATGTCTGAACCCCACCTGCCGCCTCCTCCTCCACCGCTCTACAAGCAGCGGTCGTGGTCCCCGGACGCATACCGCGACGAGGCATGGCACCGCCGCAAGAAAATCAACGGCAGATCGAACCTCCAGCGGAGCAAAAGCGTCACTGACGAAGACCTCGACGAGTTGAAAGCCTGCATCGAGCTAGGGTTCGGGTTTGACTCGCCCGAGTCGGATAAGCGACTCGCTGACACTTTTCCCGCGTTGGGACTTTACTACGCGGTTAACAAGAAGTACTGTGACTCGGTTTCTAATAAGTCTACGGCGACTACAACGACGTCGTTTTCCCCGACGCCGTCCGAGTGCGACTCGGCGACCTCCCCCATCGGCAGCCCCCATACCATTATCGGACCAGGAGATACGCCGCAGACGGTGAAGACGCGGCTGAGGCAGTGGGCCCAGGTGGTTGCTTGCACGGTGAGACAGGGTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

157

Amino Acids

17.27

Weight (kDa)

8.35

Isoelectric Point (pI)

86.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 47 - 103 2.6e-27 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50350 AT3G50350
fragaria_vesca FvH4_1g16050
malus_domestica MD02G1171900.v1.1 MD15G1283800.v1.1
prunus_persica Prupe.7G133400_v2.0.a1
pyrus_communis pycom02g13860 pycom15g24680
rosa_chinensis RchiOBHm_Chr2g0105661
rosa_laevigata RLG00000017447
rosa_multiflora Rmu_sc0001051.1_g000050
rosa_roxburghii Rroxscaffold_2G00138010
rosa_rugosa Rorug02G0129100
rosa_samantha Rh2AG180700 Rh2BG189600 Rh2CG184900 Rh2DG186700
rosa_wichuraiana Rw2G014120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 24
AatII GACGTC 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 87
AccBSI CCGCTC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 296
AccII CGCG 4 cut(s) 75, 239, 257, 418
AcyI GRCGYC 2 cut(s) 314, 329
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 79, 242
AgsI TTSAA 1 cut(s) 166
AjnI CCWGG 2 cut(s) 388, 435
AluBI AGCT 1 cut(s) 181
AluI AGCT 1 cut(s) 181
Alw26I GTCTC 1 cut(s) 451
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 1 cut(s) 432
ApaI GGGCCC 1 cut(s) 436
ApeKI GCWGC 2 cut(s) 46, 366
AspS9I GGNCC 4 cut(s) 57, 386, 432, 433
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 421, 466
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 2 cut(s) 57, 386
BaeGI GKGCMC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 436
BbsI GAAGAC 2 cut(s) 156, 419
BbvCI CCTCAGC 1 cut(s) 422
BbvI GCAGC 2 cut(s) 58, 378
BccI CCATC 1 cut(s) 368
BceAI ACGGC 3 cut(s) 124, 315, 316
BciT130I CCWGG 2 cut(s) 390, 437
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 24
BisI GCNGC 6 cut(s) 20, 47, 94, 367, 401, 419
BlsI GCNGC 6 cut(s) 21, 48, 95, 368, 402, 420
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 3 cut(s) 62, 390, 437
Bme18I GGWCC 2 cut(s) 57, 386
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 4 cut(s) 57, 386, 432, 433
BmiI GGNNCC 3 cut(s) 59, 89, 434
BmrFI CCNGG 3 cut(s) 62, 390, 437
BmsI GCATC 1 cut(s) 183
BpiI GAAGAC 2 cut(s) 156, 419
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpmI CTGGAG 1 cut(s) 107
Bpu10I CCTNAGC 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 62
BsaHI GRCGYC 2 cut(s) 314, 329
BsaJI CCNNGG 2 cut(s) 60, 435
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 242
BseBI CCWGG 2 cut(s) 390, 437
BseDI CCNNGG 2 cut(s) 60, 435
BseLI CCNNNNNNNGG 2 cut(s) 79, 242
BseMII CTCAG 1 cut(s) 413
BseRI GAGGAG 2 cut(s) 15, 18
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 2 cut(s) 58, 378
Bsh1236I CGCG 4 cut(s) 75, 239, 257, 418
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 1 cut(s) 434
BshNI GGYRCC 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 2 cut(s) 43, 258
BslI CCNNNNNNNGG 2 cut(s) 79, 242
BsmAI GTCTC 1 cut(s) 451
BsmFI GGGAC 2 cut(s) 43, 258
BsnI GGCC 1 cut(s) 434
BsoBI CYCGRG 1 cut(s) 203
Bsp120I GGGCCC 1 cut(s) 432
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 1 cut(s) 113
BspANI GGCC 1 cut(s) 434
BspCNI CTCAG 1 cut(s) 414
BspFNI CGCG 4 cut(s) 75, 239, 257, 418
BspLI GGNNCC 3 cut(s) 59, 89, 434
BspMI ACCTGC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 87
BsrBI CCGCTC 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 60, 435
BssMI GATC 1 cut(s) 113
BssNI GRCGYC 2 cut(s) 314, 329
Bst2UI CCWGG 2 cut(s) 390, 437
Bst4CI ACNGT 3 cut(s) 275, 409, 454
BstACI GRCGYC 2 cut(s) 314, 329
BstC8I GCNNGC 2 cut(s) 172, 448
BstDEI CTNAG 1 cut(s) 422
BstFNI CGCG 4 cut(s) 75, 239, 257, 418
BstKTI GATC 1 cut(s) 116
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 1 cut(s) 113
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 2 cut(s) 43, 424
BstNI CCWGG 2 cut(s) 390, 437
BstSCI CCNGG 3 cut(s) 60, 388, 435
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 4 cut(s) 75, 239, 257, 418
BstV1I GCAGC 2 cut(s) 58, 378
BstV2I GAAGAC 2 cut(s) 156, 419
BsuRI GGCC 1 cut(s) 434
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 2 cut(s) 141, 434
BveI ACCTGC 1 cut(s) 24
Cac8I GCNNGC 2 cut(s) 172, 448
Cfr13I GGNCC 4 cut(s) 57, 386, 432, 433
CseI GACGC 4 cut(s) 74, 127, 337, 424
Csp6I GTAC 1 cut(s) 271
CviAII CATG 1 cut(s) 84
CviJI RGCY 5 cut(s) 170, 181, 369, 421, 434
CviKI_1 RGCY 5 cut(s) 170, 181, 369, 421, 434
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 1 cut(s) 422
DpnI GATC 1 cut(s) 115
DpnII GATC 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 2 cut(s) 57, 386
Eco88I CYCGRG 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 388, 435
EcoT38I GRGCYC 1 cut(s) 436
FaeI CATG 1 cut(s) 87
FaiI YATR 3 cut(s) 70, 85, 375
FaqI GGGAC 2 cut(s) 43, 258
FatI CATG 1 cut(s) 83
FauI CCCGC 1 cut(s) 244
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 6 cut(s) 20, 47, 94, 367, 401, 419
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 6 cut(s) 20, 47, 94, 367, 401, 419
FspBI CTAG 1 cut(s) 182
GluI GCNGC 6 cut(s) 20, 47, 94, 367, 401, 419
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 1 cut(s) 434
HapII CCGG 1 cut(s) 62
HgaI GACGC 4 cut(s) 74, 127, 337, 424
Hin1I GRCGYC 2 cut(s) 314, 329
Hin1II CATG 1 cut(s) 87
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 5 cut(s) 197, 206, 219, 278, 344
HpaI GTTAAC 1 cut(s) 262
HpaII CCGG 1 cut(s) 62
HphI GGTGA 2 cut(s) 421, 466
Hpy166II GTNNAC 2 cut(s) 262, 297
Hpy188I TCNGA 4 cut(s) 7, 211, 337, 386
Hpy188III TCNNGA 1 cut(s) 468
Hpy8I GTNNAC 2 cut(s) 262, 297
Hpy99I CGWCG 5 cut(s) 80, 161, 316, 319, 331
HpyCH4III ACNGT 3 cut(s) 275, 409, 454
HpyCH4IV ACGT 1 cut(s) 314
HpyCH4V TGCA 2 cut(s) 174, 450
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 424
HpyF3I CTNAG 1 cut(s) 422
HpySE526I ACGT 1 cut(s) 314
Hsp92I GRCGYC 2 cut(s) 314, 329
Hsp92II CATG 1 cut(s) 87
KspAI GTTAAC 1 cut(s) 262
Kzo9I GATC 1 cut(s) 113
LmnI GCTCC 1 cut(s) 129
LpnPI CCDG 9 cut(s) 29, 75, 137, 184, 375, 402, 422, 446, 449
Lsp1109I GCAGC 2 cut(s) 58, 378
LweI GCATC 1 cut(s) 183
MaeI CTAG 1 cut(s) 182
MaeII ACGT 1 cut(s) 314
MaeIII GTNAC 2 cut(s) 139, 275
MalI GATC 1 cut(s) 115
MbiI CCGCTC 1 cut(s) 37
MboI GATC 1 cut(s) 113
MboII GAAGA 2 cut(s) 161, 424
MhlI GDGCHC 1 cut(s) 436
MlyI GAGTC 5 cut(s) 191, 213, 215, 272, 338
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 8 cut(s) 33, 36, 39, 73, 131, 164, 364, 417
MseI TTAA 1 cut(s) 261
MspA1I CMGCKG 2 cut(s) 49, 127
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 3 cut(s) 62, 390, 437
MvaI CCWGG 2 cut(s) 390, 437
MvnI CGCG 4 cut(s) 75, 239, 257, 418
MwoI GCNNNNNNNGC 2 cut(s) 43, 424
NciI CCSGG 1 cut(s) 62
NdeII GATC 1 cut(s) 113
NlaIII CATG 1 cut(s) 87
NlaIV GGNNCC 3 cut(s) 59, 89, 434
NmeAIII GCCGAG 1 cut(s) 326
NmuCI GTSAC 2 cut(s) 139, 275
PaqCI CACCTGC 1 cut(s) 24
PcsI WCGNNNNNNNCGW 3 cut(s) 72, 153, 323
PflFI GACNNNGTC 1 cut(s) 331
PkrI GCNGC 6 cut(s) 21, 48, 95, 368, 402, 420
PleI GAGTC 5 cut(s) 191, 213, 214, 272, 338
PpsI GAGTC 5 cut(s) 191, 213, 214, 272, 338
Psp6I CCWGG 2 cut(s) 388, 435
PspGI CCWGG 2 cut(s) 388, 435
PspN4I GGNNCC 3 cut(s) 59, 89, 434
PspOMI GGGCCC 1 cut(s) 432
PspPI GGNCC 4 cut(s) 57, 386, 432, 433
PsyI GACNNNGTC 1 cut(s) 331
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 6 cut(s) 20, 47, 94, 367, 401, 419
Sau3AI GATC 1 cut(s) 113
Sau96I GGNCC 4 cut(s) 57, 386, 432, 433
ScaI AGTACT 1 cut(s) 272
SchI GAGTC 5 cut(s) 191, 213, 215, 272, 338
ScrFI CCNGG 3 cut(s) 62, 390, 437
SduI GDGCHC 1 cut(s) 436
SetI ASST 7 cut(s) 18, 123, 156, 183, 317, 356, 441
SfaNI GCATC 1 cut(s) 183
SinI GGWCC 2 cut(s) 57, 386
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 3 cut(s) 60, 388, 435
TaaI ACNGT 3 cut(s) 275, 409, 454
TaiI ACGT 1 cut(s) 317
TaqI TCGA 3 cut(s) 116, 156, 177
TatI WGTACW 1 cut(s) 270
TauI GCSGC 4 cut(s) 22, 96, 403, 421
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 148, 434
TseFI GTSAC 2 cut(s) 139, 275
TseI GCWGC 2 cut(s) 46, 366
Tsp45I GTSAC 2 cut(s) 139, 275
TspRI CASTG 2 cut(s) 148, 434
Tth111I GACNNNGTC 1 cut(s) 331
VpaK11BI GGWCC 2 cut(s) 57, 386
XmiI GTMKAC 1 cut(s) 296
XspI CTAG 1 cut(s) 182
ZraI GACGTC 1 cut(s) 315
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.