pycom15g24680

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012887
Physical Location & Seq
Reverse (-)
18935887 .. 18936485
599 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g24680.2

Sequence Viewer

Length: 468 bp
ATGTCTGAACCCCACGCGCCGCCTCCTCCACCGCTCTACAAGCAGCGGTCGTGGTCCCCGGACGCATACCGCGACGAGGCATGGCACCGCCGCAAGAAAATCAACGGCAGATCGATCCTCCAGCGGAGCAAGAGCGTCACCGACGAAGATCTCGACGAGTTGAAAGCCTGCATCGAGCTAGGGTTCGGGTTTGACTCGCCCGAGACCGATCAGCGACTCACTAACACTTTTCCCGCGTTGGGACTCTACTACGCGGTTAACAAGAGTTACTGTGACTCGGTTTCTAATAAGTCTACTGGGACTCCAACGTCGCTGTTTTCGCCGACGCCGTCTGAGTGCGACTCGGTGCCCTCCCCCATCGGCAGCCCCCATACCATTTTCGGACCCGGAGATACGCCGCAGACGGTGAAGACGCGGCTGAGGCAGTGGGCACAGGTGGTTGCTTGCACGGTGAGGCAGAGTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

156

Amino Acids

17.21

Weight (kDa)

7.67

Isoelectric Point (pI)

89.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50350 AT3G50350
fragaria_vesca FvH4_1g16050
malus_domestica MD02G1171900.v1.1 MD15G1283800.v1.1
prunus_persica Prupe.7G133400_v2.0.a1
pyrus_communis pycom02g13860 pycom15g24680
rosa_chinensis RchiOBHm_Chr2g0105661
rosa_laevigata RLG00000017447
rosa_multiflora Rmu_sc0001051.1_g000050
rosa_roxburghii Rroxscaffold_2G00138010
rosa_rugosa Rorug02G0129100
rosa_samantha Rh2AG180700 Rh2BG189600 Rh2CG184900 Rh2DG186700
rosa_wichuraiana Rw2G014120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
AccB1I GGYRCC 2 cut(s) 84, 346
AccBSI CCGCTC 1 cut(s) 34
AccI GTMKAC 1 cut(s) 293
AccII CGCG 5 cut(s) 17, 72, 236, 254, 415
AclWI GGATC 1 cut(s) 109
AcyI GRCGYC 1 cut(s) 326
AfiI CCNNNNNNNGG 2 cut(s) 76, 239
AgsI TTSAA 1 cut(s) 163
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 200
ApeKI GCWGC 2 cut(s) 43, 363
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 54, 383
AsuC2I CCSGG 2 cut(s) 59, 387
AsuHPI GGTGA 3 cut(s) 130, 418, 463
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 2 cut(s) 54, 383
BaeGI GKGCMC 2 cut(s) 351, 433
BanI GGYRCC 2 cut(s) 84, 346
BbsI GAAGAC 1 cut(s) 416
BbvCI CCTCAGC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 55, 375
BccI CCATC 1 cut(s) 365
BceAI ACGGC 2 cut(s) 121, 313
BcnI CCSGG 2 cut(s) 59, 387
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 1 cut(s) 179
BglII AGATCT 1 cut(s) 148
BisI GCNGC 6 cut(s) 20, 44, 91, 364, 398, 416
BlsI GCNGC 6 cut(s) 21, 45, 92, 365, 399, 417
Bme1390I CCNGG 2 cut(s) 59, 387
Bme18I GGWCC 2 cut(s) 54, 383
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 2 cut(s) 54, 383
BmiI GGNNCC 4 cut(s) 56, 86, 348, 385
BmrFI CCNGG 2 cut(s) 59, 387
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 1 cut(s) 180
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 104
Bpu10I CCTNAGC 1 cut(s) 419
BpuMI CCSGG 2 cut(s) 59, 387
Bsa29I ATCGAT 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 326
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 57
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 239
Bse1I ACTGG 1 cut(s) 301
BseCI ATCGAT 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 57
BseLI CCNNNNNNNGG 2 cut(s) 76, 239
BseMII CTCAG 2 cut(s) 324, 410
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 15
BseSI GKGCMC 2 cut(s) 351, 433
BseXI GCAGC 2 cut(s) 55, 375
Bsh1236I CGCG 5 cut(s) 17, 72, 236, 254, 415
Bsh1285I CGRYCG 1 cut(s) 50
BshNI GGYRCC 2 cut(s) 84, 346
BshVI ATCGAT 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 2 cut(s) 59, 387
BslFI GGGAC 3 cut(s) 40, 255, 313
BslI CCNNNNNNNGG 2 cut(s) 76, 239
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 3 cut(s) 40, 255, 313
Bso31I GGTCTC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 351, 433
Bsp143I GATC 4 cut(s) 110, 114, 148, 208
BspCNI CTCAG 2 cut(s) 325, 411
BspDI ATCGAT 1 cut(s) 113
BspFNI CGCG 5 cut(s) 17, 72, 236, 254, 415
BspLI GGNNCC 4 cut(s) 56, 86, 348, 385
BspPI GGATC 1 cut(s) 109
BspT107I GGYRCC 2 cut(s) 84, 346
BspTNI GGTCTC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 34
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 4 cut(s) 110, 114, 148, 208
BssNI GRCGYC 1 cut(s) 326
Bst4CI ACNGT 3 cut(s) 272, 406, 451
BstACI GRCGYC 1 cut(s) 326
BstC8I GCNNGC 2 cut(s) 169, 445
BstDEI CTNAG 2 cut(s) 333, 419
BstFNI CGCG 5 cut(s) 17, 72, 236, 254, 415
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 4 cut(s) 113, 117, 151, 211
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 4 cut(s) 110, 114, 148, 208
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 3 cut(s) 40, 319, 421
BstSCI CCNGG 2 cut(s) 57, 385
BstSLI GKGCMC 2 cut(s) 351, 433
BstUI CGCG 5 cut(s) 17, 72, 236, 254, 415
BstV1I GCAGC 2 cut(s) 55, 375
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 113
BsuTUI ATCGAT 1 cut(s) 113
BtsI GCAGTG 1 cut(s) 431
BtsIMutI CAGTG 1 cut(s) 431
Cac8I GCNNGC 2 cut(s) 169, 445
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 54, 383
ClaI ATCGAT 1 cut(s) 113
CseI GACGC 4 cut(s) 71, 124, 334, 421
CviAII CATG 1 cut(s) 81
CviJI RGCY 4 cut(s) 167, 178, 366, 418
CviKI_1 RGCY 4 cut(s) 167, 178, 366, 418
DdeI CTNAG 2 cut(s) 333, 419
DpnI GATC 4 cut(s) 112, 116, 150, 210
DpnII GATC 4 cut(s) 110, 114, 148, 208
DrdI GACNNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 197
Eco47I GGWCC 2 cut(s) 54, 383
Eco88I CYCGRG 1 cut(s) 200
FaeI CATG 1 cut(s) 84
FaiI YATR 3 cut(s) 67, 82, 372
FaqI GGGAC 3 cut(s) 40, 255, 313
FatI CATG 1 cut(s) 80
FauI CCCGC 1 cut(s) 241
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 6 cut(s) 20, 44, 91, 364, 398, 416
Fsp4HI GCNGC 6 cut(s) 20, 44, 91, 364, 398, 416
FspBI CTAG 1 cut(s) 179
GlaI GCGC 1 cut(s) 18
GluI GCNGC 6 cut(s) 20, 44, 91, 364, 398, 416
GsuI CTGGAG 1 cut(s) 104
HapII CCGG 2 cut(s) 59, 387
HgaI GACGC 4 cut(s) 71, 124, 334, 421
HhaI GCGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 1 cut(s) 84
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 6 cut(s) 194, 216, 243, 275, 301, 341
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 2 cut(s) 59, 387
HphI GGTGA 3 cut(s) 130, 418, 463
Hpy166II GTNNAC 2 cut(s) 259, 294
Hpy188I TCNGA 3 cut(s) 7, 334, 383
Hpy188III TCNNGA 2 cut(s) 152, 465
Hpy8I GTNNAC 2 cut(s) 259, 294
Hpy99I CGWCG 5 cut(s) 77, 146, 158, 313, 328
HpyCH4III ACNGT 3 cut(s) 272, 406, 451
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 2 cut(s) 171, 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 319, 421
HpyF3I CTNAG 2 cut(s) 333, 419
HpySE526I ACGT 1 cut(s) 308
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 1 cut(s) 84
HspAI GCGC 1 cut(s) 17
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 4 cut(s) 110, 114, 148, 208
LmnI GCTCC 1 cut(s) 126
LpnPI CCDG 6 cut(s) 72, 134, 181, 282, 400, 419
Lsp1109I GCAGC 2 cut(s) 55, 375
LweI GCATC 1 cut(s) 180
MaeI CTAG 1 cut(s) 179
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 3 cut(s) 136, 266, 272
MalI GATC 4 cut(s) 112, 116, 150, 210
MbiI CCGCTC 1 cut(s) 34
MboI GATC 4 cut(s) 110, 114, 148, 208
MboII GAAGA 2 cut(s) 158, 421
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 2 cut(s) 351, 433
MlyI GAGTC 6 cut(s) 188, 210, 237, 269, 295, 335
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 7 cut(s) 33, 36, 70, 128, 361, 414, 447
MseI TTAA 1 cut(s) 258
MspA1I CMGCKG 2 cut(s) 46, 124
MspI CCGG 2 cut(s) 59, 387
MspR9I CCNGG 2 cut(s) 59, 387
MvnI CGCG 5 cut(s) 17, 72, 236, 254, 415
MwoI GCNNNNNNNGC 3 cut(s) 40, 319, 421
NciI CCSGG 2 cut(s) 59, 387
NdeII GATC 4 cut(s) 110, 114, 148, 208
NlaIII CATG 1 cut(s) 84
NlaIV GGNNCC 4 cut(s) 56, 86, 348, 385
NmuCI GTSAC 2 cut(s) 136, 272
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 150, 326
PflFI GACNNNGTC 1 cut(s) 328
PkrI GCNGC 6 cut(s) 21, 45, 92, 365, 399, 417
PleI GAGTC 6 cut(s) 188, 210, 237, 269, 295, 335
PpsI GAGTC 6 cut(s) 188, 210, 237, 269, 295, 335
PspN4I GGNNCC 4 cut(s) 56, 86, 348, 385
PspPI GGNCC 2 cut(s) 54, 383
PsuI RGATCY 1 cut(s) 148
PsyI GACNNNGTC 1 cut(s) 328
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 6 cut(s) 20, 44, 91, 364, 398, 416
Sau3AI GATC 4 cut(s) 110, 114, 148, 208
Sau96I GGNCC 2 cut(s) 54, 383
SchI GAGTC 6 cut(s) 188, 210, 237, 269, 295, 335
ScrFI CCNGG 2 cut(s) 59, 387
SduI GDGCHC 2 cut(s) 351, 433
SetI ASST 3 cut(s) 180, 311, 438
SfaNI GCATC 1 cut(s) 180
SinI GGWCC 2 cut(s) 54, 383
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 57, 385
TaaI ACNGT 3 cut(s) 272, 406, 451
TaiI ACGT 1 cut(s) 311
TaqI TCGA 3 cut(s) 113, 153, 174
TaqII GACCGA 1 cut(s) 221
TauI GCSGC 4 cut(s) 22, 93, 400, 418
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 431
TseFI GTSAC 2 cut(s) 136, 272
TseI GCWGC 2 cut(s) 43, 363
Tsp45I GTSAC 2 cut(s) 136, 272
TspRI CASTG 1 cut(s) 431
Tth111I GACNNNGTC 1 cut(s) 328
VpaK11BI GGWCC 2 cut(s) 54, 383
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.