AT3G51660

macrophage migration inhibitory factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014219
Physical Location & Seq
Reverse (-)
19163995 .. 19165322
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G51660.1

Sequence Viewer

Length: 339 bp
ATGCCTTGTCTTTACATTACAACAAACGTCAATTTTGACGGCGTTAACACCGATCCGTTCTACTCCGAAGTCACCAAAGCCGTCGCTTCCATCGTCGGACGACCTCAAAACTTAGTGATGGTGGTGTTGAAGGGATCAGTAGAGATAGTATTTGGAGGGAACAAAGAAGCAGCTGCATATGCAGAGATTGTGTCAATGGGAGGCATCACCAAACAAGTTAAGAGAGAACTCATAGCGACCGTTGGTTCTATTCTTCACACTCATTTTTCTATTCATCCCACTCGTTTTATCTTTAAAGTTTTTGATATTAATTCTTTGCCTCTTCCTTCTAAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

112

Amino Acids

12.21

Weight (kDa)

8.82

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 105 1.1e-12 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51660
fragaria_vesca FvH4_6g10610
malus_domestica MD04G1166700.v1.1 MD12G1179700.v1.1
prunus_persica Prupe.6G284900_v2.0.a1
pyrus_communis pycom04g14810 pycom12g16980
rosa_chinensis RchiOBHm_Chr3g0461611
rosa_laevigata RLG00000024914
rosa_multiflora Rmu_sc0003578.1_g000004
rosa_roxburghii Rroxscaffold_6G00418100 Rroxscaffold_6G00418470
rosa_rugosa Rorug03G0052400
rosa_samantha Rh3AG110500 Rh3CG115600 Rh3DG115500
rosa_wichuraiana Rw3G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 47, 142
AgsI TTSAA 1 cut(s) 130
AluBI AGCT 1 cut(s) 173
AluI AGCT 1 cut(s) 173
AlwI GGATC 2 cut(s) 47, 142
ApeKI GCWGC 2 cut(s) 170, 173
AseI ATTAAT 1 cut(s) 309
AsuHPI GGTGA 2 cut(s) 64, 199
BbvI GCAGC 2 cut(s) 160, 182
BccI CCATC 2 cut(s) 98, 112
BceAI ACGGC 2 cut(s) 55, 65
BisI GCNGC 2 cut(s) 171, 174
BlsI GCNGC 2 cut(s) 172, 175
BmsI GCATC 1 cut(s) 213
BseGI GGATG 1 cut(s) 274
BseXI GCAGC 2 cut(s) 160, 182
Bsh1285I CGRYCG 1 cut(s) 240
BsiEI CGRYCG 1 cut(s) 240
Bsp143I GATC 2 cut(s) 52, 134
BspPI GGATC 2 cut(s) 47, 142
BssMI GATC 2 cut(s) 52, 134
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 327
BstDEI CTNAG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 274
BstKTI GATC 2 cut(s) 55, 137
BstMBI GATC 2 cut(s) 52, 134
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstV1I GCAGC 2 cut(s) 160, 182
BtsCI GGATG 1 cut(s) 274
CviJI RGCY 2 cut(s) 80, 173
CviKI_1 RGCY 2 cut(s) 80, 173
DdeI CTNAG 1 cut(s) 112
DpnI GATC 2 cut(s) 54, 136
DpnII GATC 2 cut(s) 52, 134
DraI TTTAAA 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
FaiI YATR 3 cut(s) 178, 180, 233
FauNDI CATATG 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 171, 174
FokI GGATG 1 cut(s) 261
Fsp4HI GCNGC 2 cut(s) 171, 174
GluI GCNGC 2 cut(s) 171, 174
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HpaI GTTAAC 1 cut(s) 46
HphI GGTGA 2 cut(s) 64, 199
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 67, 98
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 2 cut(s) 86, 98
HpyAV CCTTC 2 cut(s) 124, 336
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 2 cut(s) 176, 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 27
KspAI GTTAAC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 52, 134
Lsp1109I GCAGC 2 cut(s) 160, 182
LweI GCATC 1 cut(s) 213
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 70
MalI GATC 2 cut(s) 54, 136
MboI GATC 2 cut(s) 52, 134
MboII GAAGA 2 cut(s) 245, 314
MluCI AATT 2 cut(s) 31, 310
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 4 cut(s) 114, 149, 194, 330
MseI TTAA 4 cut(s) 45, 219, 294, 309
MspA1I CMGCKG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeI CATATG 1 cut(s) 178
NdeII GATC 2 cut(s) 52, 134
NmuCI GTSAC 1 cut(s) 70
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PkrI GCNGC 2 cut(s) 172, 175
PshBI ATTAAT 1 cut(s) 309
PvuII CAGCTG 1 cut(s) 173
SaqAI TTAA 4 cut(s) 45, 219, 294, 309
SatI GCNGC 2 cut(s) 171, 174
Sau3AI GATC 2 cut(s) 52, 134
SetI ASST 3 cut(s) 30, 106, 175
SfaNI GCATC 1 cut(s) 213
SgeI CNNG 3 cut(s) 18, 227, 294
Sse9I AATT 2 cut(s) 31, 310
TaaI ACNGT 1 cut(s) 241
TaiI ACGT 1 cut(s) 30
TasI AATT 2 cut(s) 31, 310
Tru1I TTAA 4 cut(s) 45, 219, 294, 309
Tru9I TTAA 4 cut(s) 45, 219, 294, 309
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 2 cut(s) 170, 173
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 263
TspGWI ACGGA 1 cut(s) 45
VspI ATTAAT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.