pycom04g14810

Macrophage migration inhibitory

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014219
Physical Location & Seq
Reverse (-)
17587327 .. 17588895
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g14810.1

Sequence Viewer

Length: 339 bp
ATGCCTTGCCTTTATATCTCCACCAACGTCAACCTTGACGGAGTCGACACTGATTCCATCTTTTCCGAAGCCACCAAAGCCATCTCTGCCATTACCGGAAAGCCCGAAAATTTTGTGATGGTGGTACTTAAGGGATCGGTGCCCATATCGTTCGGGAAGAGTACCACTATACCGGCGTCATATGGGGAGTTAGTAGCAATGGGTGGCATTACCACCACCGTGAAGAGGCAACTGATCGCCACTCTCGGCACGATTTTTGAGTCCAAGCTTTCGATCCCGAAAACCCGATTTTTTCTTAAAGTAGTTGATTTAAGTACTCCTACAGGTTCTAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

113

Amino Acids

11.87

Weight (kDa)

9.17

Isoelectric Point (pI)

12.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 106 3.7e-10 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51660
fragaria_vesca FvH4_6g10610
malus_domestica MD04G1166700.v1.1 MD12G1179700.v1.1
prunus_persica Prupe.6G284900_v2.0.a1
pyrus_communis pycom04g14810 pycom12g16980
rosa_chinensis RchiOBHm_Chr3g0461611
rosa_laevigata RLG00000024914
rosa_multiflora Rmu_sc0003578.1_g000004
rosa_roxburghii Rroxscaffold_6G00418100 Rroxscaffold_6G00418470
rosa_rugosa Rorug03G0052400
rosa_samantha Rh3AG110500 Rh3CG115600 Rh3DG115500
rosa_wichuraiana Rw3G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 139
AccI GTMKAC 1 cut(s) 45
AclWI GGATC 2 cut(s) 142, 268
AcsI RAATTY 1 cut(s) 109
AcyI GRCGYC 1 cut(s) 176
AfaI GTAC 3 cut(s) 126, 163, 316
AfiI CCNNNNNNNGG 1 cut(s) 225
AflII CTTAAG 1 cut(s) 128
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 1 cut(s) 268
AluI AGCT 1 cut(s) 268
AlwI GGATC 2 cut(s) 142, 268
ApoI RAATTY 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 139
BccI CCATC 3 cut(s) 65, 89, 112
BfmI CTRYAG 1 cut(s) 321
BfrI CTTAAG 1 cut(s) 128
BmcAI AGTACT 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 176
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse118I RCCGGY 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 204
BseSI GKGCMC 1 cut(s) 144
BshNI GGYRCC 1 cut(s) 139
BsiSI CCGG 2 cut(s) 96, 173
BslI CCNNNNNNNGG 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 134, 234, 273
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 2 cut(s) 142, 268
BspT107I GGYRCC 1 cut(s) 139
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 172
BssMI GATC 3 cut(s) 134, 234, 273
BssNI GRCGYC 1 cut(s) 176
Bst4CI ACNGT 2 cut(s) 220, 336
Bst6I CTCTTC 2 cut(s) 152, 218
BstACI GRCGYC 1 cut(s) 176
BstAFI CTTAAG 1 cut(s) 128
BstKTI GATC 3 cut(s) 137, 237, 276
BstMBI GATC 3 cut(s) 134, 234, 273
BstMWI GCNNNNNNNGC 2 cut(s) 77, 86
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 172
CseI GACGC 1 cut(s) 165
Csp6I GTAC 3 cut(s) 125, 162, 315
CviJI RGCY 4 cut(s) 71, 80, 103, 268
CviKI_1 RGCY 4 cut(s) 71, 80, 103, 268
CviQI GTAC 3 cut(s) 125, 162, 315
DpnI GATC 3 cut(s) 136, 236, 275
DpnII GATC 3 cut(s) 134, 234, 273
Eam1104I CTCTTC 2 cut(s) 152, 218
EarI CTCTTC 2 cut(s) 152, 218
FaiI YATR 5 cut(s) 15, 146, 170, 181, 183
FauNDI CATATG 1 cut(s) 181
FblI GTMKAC 1 cut(s) 45
HapII CCGG 2 cut(s) 96, 173
HgaI GACGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 176
HincII GTYRAC 2 cut(s) 31, 46
HindII GTYRAC 2 cut(s) 31, 46
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 3 cut(s) 42, 53, 260
HpaII CCGG 2 cut(s) 96, 173
Hpy166II GTNNAC 2 cut(s) 31, 46
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 2 cut(s) 154, 277
Hpy8I GTNNAC 2 cut(s) 31, 46
HpyCH4III ACNGT 2 cut(s) 220, 336
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 86
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 176
Kzo9I GATC 3 cut(s) 134, 234, 273
LpnPI CCDG 3 cut(s) 109, 186, 309
MaeII ACGT 1 cut(s) 27
MalI GATC 3 cut(s) 136, 236, 275
MboI GATC 3 cut(s) 134, 234, 273
MboII GAAGA 2 cut(s) 169, 235
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 1 cut(s) 109
MlyI GAGTC 2 cut(s) 51, 269
MnlI CCTC 1 cut(s) 219
MseI TTAA 3 cut(s) 129, 297, 311
MslI CAYNNNNRTG 1 cut(s) 218
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 2 cut(s) 96, 173
MwoI GCNNNNNNNGC 2 cut(s) 77, 86
NdeI CATATG 1 cut(s) 181
NdeII GATC 3 cut(s) 134, 234, 273
NlaIV GGNNCC 1 cut(s) 141
NmeAIII GCCGAG 1 cut(s) 225
OliI CACNNNNGTG 1 cut(s) 218
PfeI GAWTC 1 cut(s) 53
PflFI GACNNNGTC 1 cut(s) 41
PleI GAGTC 2 cut(s) 50, 268
PpsI GAGTC 2 cut(s) 50, 268
PspN4I GGNNCC 1 cut(s) 141
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 3 cut(s) 126, 163, 316
RsaNI GTAC 3 cut(s) 125, 162, 315
RseI CAYNNNNRTG 1 cut(s) 218
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 3 cut(s) 129, 297, 311
Sau3AI GATC 3 cut(s) 134, 234, 273
ScaI AGTACT 1 cut(s) 316
SchI GAGTC 2 cut(s) 51, 269
SduI GDGCHC 1 cut(s) 144
SetI ASST 4 cut(s) 30, 36, 270, 328
SfcI CTRYAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 109
TaaI ACNGT 2 cut(s) 220, 336
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 45, 272
TasI AATT 1 cut(s) 109
TatI WGTACW 1 cut(s) 314
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 3 cut(s) 129, 297, 311
Tru9I TTAA 3 cut(s) 129, 297, 311
TscAI CASTG 1 cut(s) 55
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 1 cut(s) 55
Tth111I GACNNNGTC 1 cut(s) 41
Vha464I CTTAAG 1 cut(s) 128
XapI RAATTY 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.